JPH04500311A - Method for aseptically rooting in vitro propagated material for horticultural and agricultural purposes - Google Patents
Method for aseptically rooting in vitro propagated material for horticultural and agricultural purposesInfo
- Publication number
- JPH04500311A JPH04500311A JP2509064A JP50906490A JPH04500311A JP H04500311 A JPH04500311 A JP H04500311A JP 2509064 A JP2509064 A JP 2509064A JP 50906490 A JP50906490 A JP 50906490A JP H04500311 A JPH04500311 A JP H04500311A
- Authority
- JP
- Japan
- Prior art keywords
- rooting
- horticultural
- aseptically
- artificial material
- agricultural purposes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/18—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing inorganic fibres, e.g. mineral wool
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/40—Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure
- A01G24/48—Growth substrates; Culture media; Apparatus or methods therefor characterised by their structure containing foam or presenting a foam structure
Landscapes
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Hydroponics (AREA)
- Paper (AREA)
- Valve-Gear Or Valve Arrangements (AREA)
- Polishing Bodies And Polishing Tools (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるため要約のデータは記録されません。 (57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】 発明の名称 園芸及び農業用の試験管内で繁殖された材料を無菌的に根づかせる方法技術分野 本発明は、半透過性の透明膜で成る閉鎖した外被体内で、根づかせ媒体を使って 、園芸及び農業用の試験管内で繁殖された材料を無菌的に根づかせる方法に関す る。[Detailed description of the invention] name of invention Technological field for the aseptic rooting of in vitro propagated material for horticultural and agricultural purposes The present invention uses a rooting medium within a closed envelope consisting of a semi-permeable transparent membrane. , on methods for aseptically rooting in vitro propagated material for horticultural and agricultural purposes. Ru.
背景技術 ｗｏ ８８１０６４０２は、光を伝達し、ガス交換も行うが、外気中の微生物の 汚染からシールされた半透過性の透明材料で成る外被体内に適切な媒体と共に繁 殖する組織材料を包囲することによって（植物）Ｍｉ織の栽培を行う原理を開示 している。Background technology WO 88106402 transmits light and also performs gas exchange, but it also prevents microorganisms in the outside air. It is grown with a suitable medium inside an envelope of semi-permeable transparent material sealed from contamination. Discloses the principle of cultivating (plant) Mi weave by surrounding the growing tissue material are doing.
また、従来、水、オーキシンホルモン、ミクロ塩、マクロ塩、ビタミン、蔗糖。Also, conventionally, water, auxin hormone, micro salts, macro salts, vitamins, and sucrose.
寒天のような根づかせ媒体を使って試験管内で（即ち、無菌状態のもとて）繁殖 された材料を根づかせる方法も知られている。そのような根づかせ媒体のパーセ ンテージは植物の種類によって変化し、寒天はゲル形成勧賞として作用する。特 に、上記文献はまた、同様の外被体内でゲル状の適切な媒体と共に、そのような 繁殖材料の一部（切断片）を包囲することによって、前述の方法で繁殖した植物 の組織材料から個々の苗を育成することを教示している。Propagated in vitro (i.e. under sterile conditions) using a rooting medium such as agar Methods of rooting the material are also known. Perse of such rooting media The amount varies depending on the type of plant, and the agar acts as a gel-forming agent. Special In addition, the above-mentioned document also describes the use of such a gel-like medium within a similar envelope. Plants propagated in the above-mentioned manner by enclosing part of the propagation material (cuttings) teaches growing individual seedlings from tissue material.
この従来の根づかせ法では、典型的なものでは、閉鎖端（根の先端）を有する中 空管で成る根が形成される。一般に、毛根の形成には問題がなく、根のこの先端 部分だけが栄養を吸収する場所であるので、この部分は大変重要である。根のつ いた植物を生体内（ｉｎ−ｖｌν０）環境へ移すとき、そのような根の先端は非 常に傷つき易く、初期成長が遅れたり、さらには苗が完全に死んでしまうことさ えある。その上、生体内での根の発育は、実際の植物の成長が始る前に生じてい なければならない。This traditional rooting method typically uses a medium root with a closed end (root tip). A root consisting of an empty tube is formed. In general, there are no problems with the formation of hair roots, and this tip of the root This part is very important because it is the only part that absorbs nutrients. roots When a plant is transferred to an in-vivo (in-vlν0) environment, such root tips are They are always easily damaged, resulting in delayed initial growth or even complete death of the seedlings. There is. Moreover, in vivo root development occurs before actual plant growth begins. There must be.
そこで、本発明は、生体内の環境に植物が慣れ易くし、成長過程の開始を促進さ せるために、試験管内で繁殖された植物の根系統を改良することをねらいとする 。Therefore, the present invention makes it easier for plants to get used to the in-vivo environment and promotes the initiation of the growth process. The aim is to improve the root systems of plants propagated in vitro in order to .
本発明によれば、根づかせ媒液を含浸させたスポンジ状人工物質に、根づかせる 植物をのせ、それを夕１被体内ｔこ包囲４ど）ニセによ−て、：のねみいが達成 さ１゜る。According to the present invention, roots can be rooted in a sponge-like artificial material impregnated with a rooting medium. Place the plant on top and surround it inside the body. It's 1°.
このようにすると、毛根の形成が著しく改善され、試験管内′７′の繁殖により 得られた小植物が生体内の（第１段階の）発育状態・＼の移行に対して抵抗力が 一段と強まり、特に、初期発育が実質的に改善される。In this way, the formation of hair roots is significantly improved, and the in vitro propagation The obtained plantlets are resistant to the in-vivo (first stage) developmental state/transition. In particular, early development is substantially improved.
従来、例えばロックウールのような人工材料の上で植物を生体内の状態で発育さ せること自体はよく知られている。しかしながら、これらの場合は、すでに根系 統をもった植物の（さらなる）発育に関するものである。特に、米国特許第４． １８９，８６８号は、吸湿材料で成る支持ブロック内にその根系統を有する植物 を位置づけ、そのブロックと植物の組立体を外被体内に包囲することを教示して いる。Traditionally, plants have been grown in vivo on artificial materials such as rock wool. It is well known that However, in these cases the root system is already It concerns the (further) development of plants with lineage. In particular, U.S. Pat. No. 189,868 discloses a plant having its root system within a supporting block of hygroscopic material. teachings to position the block and plant assembly within the envelope. There is.
また、この場合、植物はすでムこ根系統を形成した後に人工材料上に配置される 。Also in this case, the plants are placed on artificial materials after forming a mucoroot system. .
発明の開示 原則的には、その中に根づく予定の小植物を植えた複数の人工材料を１つの外被 体内に包囲することができる。Disclosure of invention In principle, it is possible to combine several artificial materials into one envelope with plantlets that are intended to take root in it. It can be enclosed within the body.
しかしながら、好ましくは、根づかせる小植物を植えた各人工材料を半透過性膜 で成る別々の小室内に包囲するのが好ましい。However, preferably each artificial material planted with plantlets to be rooted is covered with a semi-permeable membrane. Preferably, they are enclosed in separate chambers consisting of:
小植物を植えた人工材料を個々の小室内に分離して置けば、個々の植物が周囲の 環境の微住物で汚染されるのを防ぐばかりでなく、小植物同志の相互感染の危険 を防止することもできる。If artificial materials with small plants are placed in separate chambers, each plant will be able to grow in its surroundings. Not only does it prevent contamination with microorganisms in the environment, but it also prevents the risk of cross-infection between small plants. It can also be prevented.
半透過性膜材料は周囲の大気との酸素交換、炭酸ガス交換及び蒸気交換を可能に する。Semi-permeable membrane material allows oxygen, carbon dioxide and vapor exchange with the surrounding atmosphere do.
本発明の方法では、自然の生体内のプロセスにおいて特徴とされる光合成をすで に試験管内の段階で開始させうることか判った。これはまた、葉の上皮の形成を 促す。その結果、事実、根づき工程中にすでに硬化作用が始ることにより、その 根づいた材料を自然の保護されていない環境へ慣れさせることが著しく促進され る。The method of the present invention completely eliminates photosynthesis, which is a characteristic feature of natural biological processes. It turns out that it can be started in vitro. This also stimulates the formation of the leaf epithelium. prompt. As a result, in fact, the hardening action begins already during the rooting process, resulting in Acclimation of the rooted material to its natural, unprotected environment is significantly facilitated. Ru.
外被体と人工材料の両方を種々の形式のものとすることが可能である。所望であ れば、１本以上の小植物を植付けるような小ブロック又は円筒形本体を、そのよ うな人工材料の適切な形として使用することもできる。Both the envelope and the artificial material can be of various types. desired If so, a small block or cylindrical body into which one or more plantlets can be planted It can also be used as a suitable form of artificial material.
また、円錐形Ｃ）人工材料をそΣｒ、ｒ一対応した円錐形Ｃ）・］＼室底部内ど 、二りこ７二め７−使用するとき、効果が得られる。In addition, the conical shape C) Σr, r-corresponding conical shape C) , Niko 7 Second 7 - When used, the effect is obtained.
そのように形成された大工＋Ａ月は小室内に容易に入れる二とができ、その材料 はそれに対応した形の底部分６．二喫合し、少くともその周囲壁は小室の底部分 の周囲壁にシールさ第１ることになる。このこと：よ、発育する根を人工材ネ４ の中にとどめる作用をする。The carpenter + A moon formed in this way can be easily inserted into the small room, and the material is the bottom part of the corresponding shape 6. At least the surrounding wall is the bottom of the chamber. The first will be sealed to the surrounding wall. This thing: The growing roots are made of artificial material 4 It acts to keep it inside.
ここで本発明の方法を実施する時に使用する小室の例に関連しながら、本発明を 説明する。The present invention will now be described with reference to an example of a chamber used in carrying out the method of the present invention. explain.
図面の簡単な説明 図１は、各小室がその一連の小室に互いに接続されている複数の小室の側面図発 明を実施するための最良の形態 図１の符号１で示す小室は、例えば厚さが２５／ｕ−のポリエチレンホイルのよ うなプラスチック材料で成る連続ウェブの形に形成される。このポリエチレンホ イルは積層をなし、その層は交叉結合により相互に接続される。ポリマー積層は ０．０１／ｕｍ級の開口を有し、それによって所望の半透過性となる。−小室は ウェブを基礎の底線２に沿ってウェブを折り曲げ２それから、それを３で示す所 で熱シールすることにより形成される。底線２から互いに離れる方向へ伸長する 隣接して対をなすシール線３は複数の上方へ広がる隔室を形成し、これが適切な 小室１を構成し、これらの小室は非使用ウェブ部分４により相互に分離される。Brief description of the drawing Figure 1 shows a side view of a plurality of chambers, each chamber connected to the series of chambers. best mode of implementation The small chamber designated by numeral 1 in Fig. 1 is made of polyethylene foil with a thickness of formed into a continuous web of plastic material such as This polyethylene The layers are stacked and the layers are interconnected by cross-links. Polymer lamination is It has an aperture on the order of 0.01/um, which provides the desired semi-transparency. -The small room Fold the web along the bottom line 2 of the foundation 2 and then It is formed by heat sealing. Extend away from base line 2 Adjacent pairs of seal lines 3 form a plurality of upwardly extending compartments that It constitutes chambers 1, which are separated from each other by unused web parts 4.
そのように形成された小室１は、例えばロックウールで成形される対応した形状 をもつ人工材料５を保持するのに通しており、その人工材料には根づけ媒体が含 浸され、根づけされる小植物６がその人工材料に植付けられる。The chamber 1 thus formed can be of a corresponding shape, for example molded from rock wool. The artificial material 5 contains a rooting medium. Submerged and rooted plantlets 6 are planted in the artificial material.
人工材料５に含浸される根づけ媒体の成分は、原則的には、本発明の方法に使用 されない寒天を除けば、前述の成分と同しである。In principle, the components of the rooting medium with which the artificial material 5 is impregnated are those used in the method of the invention. The ingredients are the same as those described above, except for the agar, which is not added.
この方法を実施するにあたって、次の手順で行うのが好ましい、即ち、所定量の 液状根づけ媒体を小室１のまだ開放した上側から導入するのが好ましい。その隙 、根づけされる小植物が植付けられた乾燥した人工材Ｆ４２を挿入し、その後、 小室を符号７で示す熱シールによって閉鎖する。In carrying out this method, it is preferable to carry out the following steps: Preferably, the liquid rooting medium is introduced from the still open upper side of the chamber 1. That gap , insert the dry artificial material F42 with plantlets to be rooted, and then, The chamber is closed by a heat seal indicated at 7.
さらに、所望の無菌状態のもとで前述の操作が確実に行われるような手段を講じ る必要があることは明らかである。In addition, steps shall be taken to ensure that the aforementioned operations are carried out under the desired sterile conditions. It is clear that there is a need to
国 際 調 資 報 告 ＴＴ／ＮＬ９０．１０００Ｆ１８International research funding report TT/NL90.1000F18
Claims (3)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL8901562 | 1989-06-21 | ||
| NL8901562A NL8901562A (en) | 1989-06-21 | 1989-06-21 | PROCESS FOR THE STERILE ROOTING OF IN-VITRO MULTIPLE STARTING MATERIAL FOR HORTI AND AGRICULTURE. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH04500311A true JPH04500311A (en) | 1992-01-23 |
Family
ID=19854871
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2509064A Pending JPH04500311A (en) | 1989-06-21 | 1990-06-21 | Method for aseptically rooting in vitro propagated material for horticultural and agricultural purposes |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US5155932A (en) |
| EP (1) | EP0409298B1 (en) |
| JP (1) | JPH04500311A (en) |
| AT (1) | ATE103134T1 (en) |
| CA (1) | CA2035021A1 (en) |
| DE (1) | DE69007565D1 (en) |
| FI (1) | FI910787A0 (en) |
| HU (1) | HUT61867A (en) |
| IL (1) | IL94831A (en) |
| NL (1) | NL8901562A (en) |
| PT (1) | PT94457A (en) |
| WO (1) | WO1990015528A1 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK174990A (en) * | 1990-07-23 | 1992-01-24 | Poul Kristensen | PROCEDURE AND PLANT FOR PROMOTION OF PLANTS |
| CN107094390A (en) * | 2017-04-19 | 2017-08-29 | 上海溪昌环境工程有限公司 | A kind of new method for building sponge city |
| CN109845440B (en) * | 2018-11-12 | 2022-02-22 | 福建农林大学 | Method for promoting soil nitrate reduction by using iron film at rice root |
| MX2022002087A (en) * | 2019-08-19 | 2022-08-04 | Node Labs Inc | In-vitro photoautotrophic propagation of cannabis. |
| WO2022167173A1 (en) | 2021-02-03 | 2022-08-11 | Basf Coatings Gmbh | Method for forming a multilayer coating and object coated with such a multilayer coating |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3739522A (en) * | 1971-07-22 | 1973-06-19 | G Greenbaum | Horticultural cell system and method of manufacture |
| US3973355A (en) * | 1974-01-18 | 1976-08-10 | Agritec Co. | Self-contained hydrophilic plant growth matrix and method |
| US4034508A (en) * | 1975-08-11 | 1977-07-12 | Gravi-Mechanics Co. | Polymerized soil with growing plant and method of making and package therefor and hanger and plaque incorporating the same |
| US4189868A (en) * | 1978-02-22 | 1980-02-26 | General Mills, Inc. | Package for perishable produce |
| EP0252191B1 (en) * | 1984-12-04 | 1992-06-17 | Synthetic Substrates Limited | Synthetic substrate for use in the rooting of cuttings and the raising of seedlings and plants |
| IL80316A0 (en) * | 1985-10-17 | 1987-01-30 | Dna Plant Techn Corp | Process and apparatus for growing plantlets |
| US4908315A (en) * | 1987-03-04 | 1990-03-13 | Agristar, Inc. | Integument and method for micropropagation and tissue culturing |
-
1989
- 1989-06-21 NL NL8901562A patent/NL8901562A/en not_active Application Discontinuation
-
1990
- 1990-06-21 IL IL9483190A patent/IL94831A/en not_active IP Right Cessation
- 1990-06-21 JP JP2509064A patent/JPH04500311A/en active Pending
- 1990-06-21 HU HU905369A patent/HUT61867A/en unknown
- 1990-06-21 EP EP90201635A patent/EP0409298B1/en not_active Expired - Lifetime
- 1990-06-21 DE DE90201635T patent/DE69007565D1/en not_active Expired - Lifetime
- 1990-06-21 CA CA002035021A patent/CA2035021A1/en not_active Abandoned
- 1990-06-21 WO PCT/NL1990/000088 patent/WO1990015528A1/en active Application Filing
- 1990-06-21 AT AT90201635T patent/ATE103134T1/en not_active IP Right Cessation
- 1990-06-21 PT PT94457A patent/PT94457A/en not_active Application Discontinuation
- 1990-06-21 US US07/659,294 patent/US5155932A/en not_active Expired - Fee Related
-
1991
- 1991-02-19 FI FI910787A patent/FI910787A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2035021A1 (en) | 1990-12-22 |
| FI910787A0 (en) | 1991-02-19 |
| PT94457A (en) | 1991-02-08 |
| WO1990015528A1 (en) | 1990-12-27 |
| ATE103134T1 (en) | 1994-04-15 |
| IL94831A0 (en) | 1991-04-15 |
| EP0409298B1 (en) | 1994-03-23 |
| EP0409298A1 (en) | 1991-01-23 |
| IL94831A (en) | 1994-01-25 |
| HU905369D0 (en) | 1991-12-30 |
| DE69007565D1 (en) | 1994-04-28 |
| NL8901562A (en) | 1991-01-16 |
| HUT61867A (en) | 1993-03-29 |
| US5155932A (en) | 1992-10-20 |
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