JPH0449655B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to a clinical test and relates to an apparatus for separating plasma from collected whole blood, which is a specimen sample, in a short time.

Relationship with conventional technology Conventionally, in blood biochemical and serum tests, blood is collected from the patient with a vacuum blood collection tube or syringe, and in the case of serum, blood is collected from the patient using a vacuum blood collection tube or syringe.
After standing for 30-60 minutes, in the case of plasma, add heparin.
After adding anticoagulant such as EDTA, at 3000 rpm,
Centrifugation was performed for 5 to 10 minutes, and the supernatant was used as a sample. This method requires a lot of time and manpower, and has been viewed as a problem, especially in urgent cases. On the other hand, in the conventional method, analysis values are also obtained by centrifugation at high speed for a long time, so LDH, an enzyme in blood cells,
GOT leakage into plasma, coagulation test based on generated heat,
Unlike concentration components in the human body, such as the influence on ammonia tests, evaporation by air currents, and removal of polymers, true biological information has been measured as a distorted value. There was also the problem that platelet separation was insufficient. In order to overcome these drawbacks, attempts have been made to automate centrifugation and separation using flat membranes, but there are still problems that need to be solved in terms of shortening analysis time.

Purpose of the Invention In view of the above-mentioned shortcomings of conventional devices, the present inventor has conducted intensive studies to find a plasma separation device that can easily and quickly obtain samples without damaging biological information, and as a result has developed the present invention. reached.

Structure of the Invention The plasma separation device for testing of the present invention includes a module in which a plasma filtering section made of a hollow fiber bundle having micropores and having both ends open and a blood cell component receiving tank having a plasma component flow path section are integrated in series. It has a structure in which a removable plasma component receiver can be connected to the module.

The present invention will be explained below based on the drawings.

FIG. 1 shows an embodiment of the present invention, in which a plasma component receiver is screwed and connected to a separation module case 6 for a vacuum blood collection tube containing whole blood 1 collected from a patient using a vacuum blood collection tube 2. Insert 8 from above. The separation module hollow fiber bundle 3, which passes through the plasma in whole blood, is fixed by the adhesive of the end seal band 4, but each hollow fiber is open at the end face. On the other hand, the lower end sealing band 4' has a plasma component flow path 5 open to the rear side. The separation module case has an air hole 13 in the upper part for allowing plasma to flow downward. In the blood cell component receiving tank, a plasma component channel 5 is located in the center from the upper end seal band 4' and connected to the lower receiving bottom 12. The flow path communicates between the upper side and the lower plasma component receiver.

The blood cell components that have passed through the hollow fiber bundle from the end seal band 4' of the receiver are collected in the blood cell component receiver. For this purpose, the housing likewise has air holes 13' in the upper part. The plasma component receiving tank 8 stores the filtered plasma components via the plasma component flow path 5. It can be separated from the separation module after separation. Also, like the other parts, there is an air hole 13' in the upper part.

To summarize the above steps in the order of operation, after inserting the separation module 6 into the vacuum blood collection tube 2 from above, turn it upside down and move the vacuum blood collection tube 2 and module 6 up and down in a piston-cylinder relationship to collect the blood cells in the whole blood. Component 7 passes through the hollow fibers and accumulates in the blood cell receptor 7. On the other hand, the plasma component is filtered through the hollow fiber 3, passes through the channel 5, and is stored in the receiving tank 8. FIG. 2 shows a case where a specimen is collected with a syringe. Although the manual separation method is described here, it can also be automated using the separation module. Also, the hollow fibers used are membrane type.

Effects of the Invention According to the present invention, plasma can be easily and quickly separated using a disposable separation membrane module without using a centrifugal separator and without damaging the sample. In addition, by incorporating it into an automatic machine, it becomes possible to eliminate the process that was previously interrupted by centrifugation by simply injecting the collected blood sample. The plasma obtained by the separator is led to an automatic analyzer, and the analysis results are output to a computer, allowing for complete automation. On the one hand, it not only allows us to respond quickly to emergency tests, but on the other hand, it is also very meaningful in that it provides truer biological information as analysis values and increases the reliability of clinical data.

[Brief explanation of the drawing]

FIG. 1 shows an embodiment of the present invention in which a vacuum blood collection tube is used, and FIG. 2 shows a case in which a syringe is used. DESCRIPTION OF SYMBOLS 1... Whole blood sample, 2... Vacuum blood collection tube, 3... Hollow fiber membrane, 4... End seal band, 5... Plasma component flow path, 6... Case, 7... Blood cell component, 8... ...Plasma component receiver, 9...Plasma, 10...Blood collection syringe, 11...Connection nozzle, 12...Blood cell component receiver, 13...Air hole.

Claims (1)

[Claims] 1. A module in which a filter section made of a hollow fiber bundle with micropores and open ends and a blood cell component receiving tank having a plasma component flow path section are integrated in series, and a plasma component detachably connected to the module. A plasma separator for testing consisting of a receiving tank.