JPH0438379B2 - - Google Patents

Description

[Detailed description of the invention]

INDUSTRIAL APPLICATION FIELD The present invention relates to a highly digestible compound feed suitable for feeding to young pigs such as piglets immediately after weaning. Prior Art Originally, in order to maintain the health of livestock and allow them to grow smoothly, feed must be broken down in the digestive tract by the action of digestive enzymes into a form that can be easily absorbed in the small and large intestines when it is ingested. . However, in young pigs, such as piglets that have just been weaned, their digestive tracts have not yet fully developed and their own digestive enzyme secretion ability is low, so conventionally, it has been used as a protein source in the feed fed to young pigs. It was not possible to use the soybean meal used in feed for adult pigs, and instead it was necessary to use highly digestible ingredients such as skim milk powder and fish meal. Under these circumstances, attempts have been made to add various proteases to feed in order to supplement piglets' ability to secrete digestive enzymes, but in general, proteases are easily degraded by pigs' stomach acids, Since the pH was different from that in the stomach of a pig, the digestive function of the protease added above could not be fully demonstrated. Purpose of the Invention The present invention has been made in view of the above-mentioned situation, and it provides a digestible feed suitable for rearing young pigs by adding and containing protease produced by microorganisms belonging to a specific genus to a compound feed. The purpose is to provide a compound feed for young pigs that is rich in nutrients. The present invention will be explained in detail below. Structure of the Invention The structural feature of the present invention is that the protease-producing bacteria belonging to the genus Rhizopus, Rhizopus niveus IAM6031, Rhizopus deremer IAM6038, and/or Rhizopus deremer IAM6040, are cultivated to achieve the highest acid resistance. suitable
A compound feed for young pigs contains a protease with a pH of around 4, and the protein source in the compound feed is derived from soybean meal. In the present invention, the proteases added to the compound feed include protease-producing bacteria Rhizopus niveus IAM6031 belonging to the genus Rhizopus, Rhizopus niveus IAM6031;
It is obtained by culturing Rhizopus deremer IAM6038 and/or Rhizopus deremer IAM6040, extracting the produced protease from the culture, precipitating the extract with an organic solvent, further purifying, and vacuum drying at low temperature. Next, an example of a method for preparing the above protease will be shown. Add water to wheat bran to make it 45-50% moisture,
After pressure steaming at 120℃ for 30 minutes and cooling, protease-producing bacteria belonging to the Rhizopus genus were pre-cooked at 25-30℃.
For about 20 hours, 10 to 20% of the preculture solution cultured in a liquid medium containing 10% wheat bran is inoculated with stirring. this
The culture obtained by culturing at 25-30℃ for 3-4 days was extracted with 5 times the amount of water, and ethanol was added to the extract to a concentration of 50-70% while stirring under cooling.
After standing at ~20°C, the resulting precipitate is washed twice with ethanol, collected by centrifugation, and vacuum-dried at about 40°C. Here, the microorganisms used for protease production include Rhizopus niveus IAM6031, Rhizopus telemer IAM6038, and Rhizopus telemer IAM6038.
There are IAM6040 strains, and these strains are known strains and can be easily obtained by third parties. The protease obtained as described above is acid-resistant and has an optimum pH of around 4, so it is not degraded by pig stomach acid. In the present invention, by adding this protease to the intended compound feed at a rate of 104,000 to 260,000 units/Kg, it is possible to enhance the digestion of the feed in the gastrointestinal tract of young pigs. The unit of protease mentioned here is that when it is made to act on milk casein adjusted to pH4 at a temperature of 37℃,
One unit (protein digestion power unit) is the amount of enzyme that causes an increase in the amount of non-protein phorin reagent colored substance equivalent to 1 μg of tyrosine per minute in the initial stage of the reaction. The compound feed in the present invention includes skim milk powder, fish meal,
It is used by blending soybean meal, wheat flour, glucose, animal oil, vitamins, minerals, and other additives. However, soybean meal is mostly used as a protein source. In conventional compound feeds for young pigs, soybean meal is rarely used as a protein source, or only a small amount can be used as described above, but in the present invention, the amount of soybean meal used can be increased. Accordingly, the amount of skim milk powder and fishmeal used can be reduced. Effects of the Invention As described above, the present invention provides a method for raising young children by adding and containing protease, which is obtained by culturing protease-producing bacteria belonging to the genus Rhizopus and has properties of acid resistance and an optimum pH of around 4, to a compound feed. Since the ability of the pig's digestive tract to digest feed can be significantly increased, there is no need to use particularly highly digestible skim milk powder or fishmeal as a protein source for compound feeds, as in the past, and even if it is used, it can be done in small quantities. This makes it possible to use readily available soybean meal, which is commonly used as a protein source in pig feed. EXAMPLES The present invention and its effects will be specifically explained below with reference to Examples. Example 1 Thirty-two piglets aged 3 to 4 weeks were tested in 4 test sections (8
A growth test was conducted by feeding the mixed feeds (No. 1 to No. 4) in the proportions shown in Table 1 to each test section. The results are shown in Table 2. The protease used in this test was an enzyme obtained by culturing Rhizopus niveus IAM6031 according to the above-mentioned preparation method.

【table】

[Table] As shown in Table 2, in the test plots (No. 1 and No. 2) fed with the protease-added feed according to the present invention, the amount of skim milk powder added as a protein source was significantly reduced; Regardless of whether soybean meal was used instead, growth was comparable to that of the test plot fed a conventional feed containing a large amount of skim milk powder (No. 4), but the test plot to which no protease was added (No. 4) showed growth.
3) results in poor growth. Example 2 28 piglets around 5 weeks old in 4 test sections (7 piglets/
A growth test was conducted by feeding each test group with a feed containing the proportions shown in Table 3. The same protease used in Example 1 was added. The results are shown in Table 4.

【table】

【table】

[Table] As shown in Table 4, the test plots (No. 1 and No. 2) in which protease was added according to the present invention were compared with the test plot (No. 4) in which the feed was mixed with conventional skim milk powder and fish meal as protein sources. ), but the growth was inferior in the test plot (No. 3) in which no protease was added.

Claims (1)

[Claims] 1 Optimal acid resistance obtained by culturing protease-producing bacteria Rhizopus niveus IAM6031, Rhizopus deremer IAM6038 and/or Rhizopus deremer IAM6040 belonging to the genus Rhizopus
A compound feed for young pigs in which protease with a pH of around 4 is added to the compound feed, and the protein source in the compound feed is derived from soybean meal.