JPH0437714B2 - - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a bacterial culture shear plate used for bacterial isolation, bacterial enrichment culture, drug susceptibility testing, and the like.

[Conventional technology]

BACKGROUND ART In bacterial testing in hospitals and the like, in order to detect pathogens that cause infectious diseases, it is necessary to isolate and culture bacteria from test materials and perform enrichment culture operations. In particular, the results of bacterial tests are becoming more important now that the pathological patterns of infectious diseases are complex and diverse, and it is becoming difficult to estimate the pathogenic bacteria from clinical findings.

However, different types of bacteria require different media for growth, so when screening for pathogens, it is necessary to prepare an appropriate culture medium for each type of bacteria that may be detected as pathogens in the test materials. .

In order to prepare various types of plate culture media using conventional bacterial culture trays, complicated operations such as dissolving the medium components, sterilization, dispensing, and coagulation are required, and each operation must be performed as sterilely as possible. Moreover, since one medium is usually dispensed per shear,
The number of sheets required per sample also increases,
In hospital laboratories, etc., where the number of specimens and the types of bacteria handled are not constant, it is virtually impossible to prepare the amount required for each use.

Therefore, many facilities have no choice but to produce or purchase the expected amount needed within a certain period at one time and store it in the refrigerator before consuming it.
In this case as well, since the product is stored or purchased with the lid simply closed, there are problems such as moisture and germs entering the product.

In addition, although drying before use is an essential operation for conventional culture media, media that are deaerated during storage or purchase can also dissolve oxygen in the air during this drying process, resulting in anaerobic conditions. When handling bacteria, it was necessary to leave them in an anaerobic chamber after drying them.

Regarding the inoculation operation, most of the conventional bacterial culture petri dishes require only one culture medium per plate, which increases the number of plates required per specimen, which inevitably makes the operation complicated. Regarding this point, there is a method of dispensing different types of culture media into a fractionated shear dish with a partition wall inside the shear dish body, but when preparing the culture medium, a step is created between each culture medium and between the shear partition wall. Therefore, the inoculation operation must be carried out for each fraction, which is the same as in the case of using one medium per shear as described above.

[Problem that the invention seeks to solve]

Therefore, this invention solves the problems that the conventional bacterial culture trays have, namely, that complicated operations are required to prepare the culture medium, and that moisture and bacteria enter the culture medium during storage and transportation. Furthermore, the present invention is intended to solve the problem that only one culture medium can be inoculated in a single inoculation operation, resulting in extremely poor work efficiency.

[Means for solving problems]

Therefore, in the present invention, the upper edge of the shear dish main body is covered with an impermeable membrane, and small holes are provided at the bottom of the shear dish. The impermeable membrane is removably attached to the upper edge of the shear main body, and is impermeable to at least water and oxygen. Furthermore, in the present invention, a partition wall having a height reaching the impermeable membrane from the bottom of the shear dish is provided, or a sealing body is provided in the small hole, if necessary.

[Effect]

As a result of the above measures, the preparation of the culture medium can be done simply and aseptically by dispensing the medium filler through the small holes provided at the bottom of the shear dish, and moisture and bacteria will not enter during storage and transportation. Moreover, since the medium is sealed without direct contact with air, deterioration of the medium due to oxidation etc. is suppressed. In addition, during the inoculation operation, by peeling off the impermeable membrane, even if the culture medium is fractionated, a flat culture surface can be obtained, so all of the fractionated culture medium can be injected at once in one operation. It is now possible to inoculate.

〔Example〕

DESCRIPTION OF THE PREFERRED EMBODIMENTS The structure of the present invention will be described below with reference to embodiments.

Reference numeral 1 denotes a shear dish main body made of a material such as plastic or glass, preferably transparent, whose upper edge is covered with a removable impermeable film 2, and a small hole 4 is provided in the bottom part 3 of the shear dish. ing.
The impermeable membrane 2 is implemented in the form of a film or a sheet, and is required to have at least waterproof properties and properties that are difficult to permeate oxygen.
Preferred materials include synthetic resin films with high oxygen gas barrier properties such as polyacrylonitrile, nylon, polymethacrylonitrile, ethylene/vinyl alcohol polymer, polyvinylidene chloride, polyethylene terephthalate, and polyvinyl chloride, but other materials may also be used. Any material may be used as long as the purpose can be achieved by laminating the above-mentioned high oxygen gas barrier resin or metal foil such as aluminum foil. The thickness of the impermeable membrane 2 varies depending on its material, but it is sufficient if it has enough strength to maintain a flat surface when filled with a medium. During this time, reinforcement can be achieved by inserting a flat reinforcing material into the gap between the impermeable membrane 2 and the lid 5, which will be described below. Furthermore, it is preferable to provide a removable sealing body 6 in the small hole 4 of the bottom part 3 of the shear dish to keep the interior of the shear dish main body 1 in a sterile state after sterilization. It goes without saying that, like the chalet, the whole chalet is provided in a sterile state. Furthermore, as in the embodiments shown in FIGS. 5 to 8, the bottom part 3 of the shear dish can have a slope from the peripheral edge of the shear dish main body 1 to the small hole 4, so that foam is generated when dispensing the culture medium. It is possible to fill the medium without any trouble.

5 is a tray lid made of a material such as plastic or glass, preferably transparent;
A ring-shaped protrusion 7 is provided on its inner peripheral edge. This protrusion 7 is to create a gap between the culture medium surface and the dish lid 5, to prevent bacterial colonies growing on the culture medium surface from adhering to the inner surface of the dish lid 5, and to prevent contact with the outside world. It is provided to maintain proper airtightness. Incidentally, the projection 7 does not necessarily have to be provided on the inner peripheral edge of the tray lid 5, but can also be provided on the upper end peripheral edge of the tray main body 1.

The shear dish main body 1 has a shear bottom part 3.
The partition wall 8 can be provided with a height that reaches the impermeable membrane 2. In this case, the small holes 4 in the bottom part 3 of the shear dish must be provided in all of the compartments 9 formed by the partition walls 8. Furthermore, it goes without saying that the shear plate bottom 3 can have a slope extending from the peripheral edge of the shear plate main body 1 to the small hole 4.

Incidentally, the bacterial culture shear dish of the present invention can be used in all types of agar culture media, except for liquid and semi-liquid media.

〔Effect of the invention〕

Since the bacterial culture petri dish of the present invention is constructed as described above, filling the culture medium into the dish dish main body 1 does not require opening the dish dish lid 5 and dispensing the medium as in the conventional case. The culture medium can be dispensed from the small hole 4 in the bottom 3 of the shear dish by turning the shear dish over. Therefore, since the dispensed medium is not opened with the shell lid 5 at the time of dispensing, there is not only less risk of bacterial contamination compared to the conventional method, but also the medium is protected from the impermeable membrane 2.
By peeling off this impermeable membrane 2 when the medium is coagulated as the bottom surface and used for inoculation and culture, there is no need for a horizontal platform for coagulating the medium as in the past, and vibrations during coagulation can be removed. No disturbance of the medium surface occurs.

Furthermore, even when storing and transporting the medium after filling it, the medium is enclosed by the impermeable membrane 2 and the shear body 1, so it does not come into contact with air. It also has the effect of preventing bacterial contamination during storage.

Similarly, when using the present invention's Chare having fractions, the height of the culture surface of the culture medium filled in each fraction is unified by the impermeable membrane 2, so that the conventional fractionated Chare culture medium is Inoculation using an automatic inoculation device such as a spiral plater (Spiral System Instruments, Inc., USA), which could not be applied due to the difference in level between each medium and the partition wall 8, is now possible, and even when inoculating manually, it is possible to inoculate once. It is possible to inoculate a large number of media using this operation.

In addition, as mentioned above, the bacterial culture shear dish of the present invention can store the medium dispensed inside while keeping it isolated from air. By injecting, a suitable medium with a lower amount of dissolved oxygen than conventional media can be provided for culturing anaerobic bacteria.

In addition, the bacterial culture shear dish of this invention can be prepared by combining appropriate media using fractionation.
It is also possible to screen for pathogens using one or several sheets.

Furthermore, by containing a drug at a predetermined concentration in the filled medium, a simple medium for drug susceptibility testing can be provided.

[Brief explanation of drawings]

FIG. 1 is a partially cutaway exploded perspective view showing an embodiment of a bacterial culture tray according to the present invention, FIG. 2 is a bottom view of the tray lid of the embodiment shown in FIG. 1, and FIG. A bottom view of the Schare main body of the embodiment shown in FIG.
Figure 4 is a cross-sectional view taken along line A-A in Figure 3;
The figure is a partially cutaway exploded perspective view showing another embodiment of the bacterial culture tray according to the present invention, FIG. 6 is a bottom view of the tray lid of the embodiment shown in FIG. 5, and FIG.
FIG. 8 is a sectional view taken along line B-B in FIG. 7, and FIG. 9 is a partially broken view showing another embodiment of the shear dish for bacterial culture according to the present invention. An exploded perspective view, FIG. 10 is a bottom view of the tray cover of the embodiment shown in FIG. 9, and FIG. 11 is a bottom view of the tray main body of the embodiment shown in FIG. 1... Sheare main body, 2... Impermeable membrane, 3...
...Shear bottom, 4...Small hole, 6...Sealing body, 8
...Bulkhead.

Claims (1)

[Claims] 1. The upper edge of the shear dish main body 1 is made removable and is covered with an impermeable membrane 2 that does not permeate at least water and oxygen, and a small hole 4 is provided in the bottom part 3 of the shear dish. Shear plate for bacterial culture. 2. The shear dish for bacterial culture according to claim 1, wherein the shear dish main body 1 has a diaphragm 8 having a height reaching the impermeable membrane 2 from the bottom part 3 of the shear dish. 3. The bacterial culture shear dish according to claim 1, wherein the small hole 4 has a removable sealing body 6.