JPH0436636A - Flow cell device - Google Patents
Flow cell deviceInfo
- Publication number
- JPH0436636A JPH0436636A JP2143458A JP14345890A JPH0436636A JP H0436636 A JPH0436636 A JP H0436636A JP 2143458 A JP2143458 A JP 2143458A JP 14345890 A JP14345890 A JP 14345890A JP H0436636 A JPH0436636 A JP H0436636A
- Authority
- JP
- Japan
- Prior art keywords
- liquid
- sample
- flow cell
- flow
- sheath
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 claims abstract description 107
- 239000002699 waste material Substances 0.000 claims abstract description 11
- 238000004140 cleaning Methods 0.000 abstract description 25
- 238000005259 measurement Methods 0.000 abstract description 10
- 238000011109 contamination Methods 0.000 abstract description 5
- 210000004027 cell Anatomy 0.000 description 36
- 239000002245 particle Substances 0.000 description 11
- 238000012360 testing method Methods 0.000 description 10
- 238000010586 diagram Methods 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は、フローサイトメトリ等の検体検査装置用フロ
ーセル装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a flow cell device for a sample testing device such as a flow cytometer.
［従来の技術］
フローサイトメトリとは、高速で流れる細胞浮遊溶液、
即ちサンプル液に例えばレーザー光を照射し、その散乱
光・蛍光による光電信号を検出し、細胞の性質・構造を
解明する装置であり、細胞化学、免疫学、血液学、腫瘍
学、遺伝学等の分野で使用されている。[Conventional technology] Flow cytometry is a method using a cell suspension solution that flows at high speed.
In other words, it is a device that irradiates a sample liquid with, for example, a laser beam, detects a photoelectric signal from the scattered light and fluorescence, and elucidates the properties and structure of cells, and is used in cytochemistry, immunology, hematology, oncology, genetics, etc. used in the field of
このフローサイトメトリ等に用いられる従来の粒子解析
装置では、フローセルの中央部の例えば２００μｍ×２
００μｍの微小な四角形断面を有する流通部内を、シー
ス液に包まれて通過する血球細胞などの被検粒子に光ビ
ームを照射し、その結果として生ずる前方及び側方散乱
光により、被検粒子の形状・大きさ・屈折率等の粒子的
性質を得ることが可能である。また、蛍光剤により染色
され得る被検粒子に対しては、照射光とほぼ直角方向の
側方散乱光から被検粒子の蛍光を検出することにより、
被検粒子を解析するための重要な情報を求めることがで
きる。In conventional particle analysis devices used for flow cytometry, etc., the central part of the flow cell is, for example, 200 μm x 2
A light beam is irradiated onto test particles such as blood cells wrapped in sheath fluid and passing through a flow section with a minute rectangular cross section of 00 μm, and the resulting forward and side scattered light is used to detect the test particles. It is possible to obtain particle properties such as shape, size, and refractive index. In addition, for test particles that can be dyed with a fluorescent agent, by detecting the fluorescence of the test particles from side scattered light in a direction approximately perpendicular to the irradiation light,
Important information for analyzing test particles can be obtained.
第４図はフローサイトメトリ等の検体検査用フローセル
装置の第１の従来例であり、抗原抗体反応や蛍光試薬に
よる染色などの処理を受けたサンプル液Ｓａはサンプル
液容器ｌに入れられ１、また蒸留水や生理食塩水等のシ
ース液ｓｈはシース液容器２に入れられている。サンプ
ル液容器１内及びシース液容器２内はそれぞれ図示しな
い加圧機構により加圧されている。そして、シースフロ
ー原理によりフローセルチャンバ３内でサンプル液Ｓａ
がシース液ｓｈに包まれて細い流れに収斂され、フロー
セル４内の流通部の中央部を通過する。このとき、サン
プル液Ｓａ中の被検粒子は分離され、１粒或いは１塊ず
つ順次に流れる。この被検粒子の流れに対して、レーザ
ー光源５から出射されたレーザー光が、シリンドリカル
レンズ６ａ、６ｂによって収斂されて照射され、被検粒
子からは散乱光や蛍光の光信号が生ずる。前方散乱光は
集光レンズ７で集束されて検出器８で検出され、また側
方散乱光や蛍光は図示しない側方の集光レンズで集束さ
れて検出器で検出される。そして、これらの検出された
信号はコンピュータで演算され各種の解析が行われる。FIG. 4 shows a first conventional example of a flow cell device for specimen testing such as flow cytometry, in which a sample liquid Sa that has undergone treatments such as antigen-antibody reaction and staining with a fluorescent reagent is placed in a sample liquid container l; Further, a sheath liquid sh such as distilled water or physiological saline is placed in a sheath liquid container 2. The inside of the sample liquid container 1 and the inside of the sheath liquid container 2 are each pressurized by a pressurizing mechanism (not shown). Then, the sample liquid Sa is
is wrapped in the sheath liquid sh and converged into a thin flow, which passes through the center of the flow section in the flow cell 4. At this time, the test particles in the sample liquid Sa are separated and sequentially flow one particle or one lump at a time. Laser light emitted from the laser light source 5 is converged and irradiated by the cylindrical lenses 6a and 6b onto the flow of the test particles, and the test particles generate optical signals of scattered light and fluorescence. Forward scattered light is focused by a condensing lens 7 and detected by a detector 8, and side scattered light and fluorescence are focused by a side condensing lens (not shown) and detected by a detector. Then, these detected signals are calculated by a computer and various analyzes are performed.
第５図は第２の従来例を示し、サンプル液容器ｌ内のサ
ンプル液Ｓａはサンプルシリンダ９により験引され、三
方ロバルブｌＯを経てサンプル溜め１１に一旦溜められ
てから、次にサンプルシリンダ９で押し出されフローセ
ルチャンバ３へと送られる。一方、シース液ｓｈはシー
スシリンダ１２によりシース液容器２から三方ロバルブ
１３を経て一旦吸引された後にフローセルチャンバ３へ
と送られる。FIG. 5 shows a second conventional example, in which the sample liquid Sa in the sample liquid container l is subtracted by the sample cylinder 9, passed through the three-way valve lO, and once stored in the sample reservoir 11, and then transferred to the sample cylinder 9. is pushed out and sent to the flow cell chamber 3. On the other hand, the sheath liquid sh is once sucked from the sheath liquid container 2 by the sheath cylinder 12 via the three-way valve 13 and then sent to the flow cell chamber 3.
［発明が解決しようとする課題１
しかしながら、上述の第１の従来例では加圧のためのコ
ンプレッサやレギュレータなどが必要であり、装置が大
きくなってしまう。また、サンプル液容器ｌ内を加圧す
るため、サンプル液容器１は気密に保持しなければなら
ず、サンプル液容器１の交換は容易ではなく、自動的に
多検体を測定するには不向きである。[Problem to be Solved by the Invention 1] However, the first conventional example described above requires a compressor, a regulator, etc. for pressurization, and the device becomes large. In addition, since the inside of the sample liquid container 1 is pressurized, the sample liquid container 1 must be kept airtight, and it is not easy to replace the sample liquid container 1, making it unsuitable for automatically measuring multiple samples. .
また、第２の従来例では、サンプル液Ｓａは吸引され三
方ロバルブ１０を通り、−旦すンプル溜め１１に溜めら
れてから、フローセルチャンバ３へと送られるため時間
的に不利であり、次のサンプル液Ｓａを流す時に前回の
測定サンプルの残液が混入してしまう、所謂コンタミネ
ーションの可能性も大きい。また、サンプル液Ｓａの吸
引量は成る程度一定であるので、サンプル液Ｓａの濃度
によっては希望する測定個数に満たなかったり、逆にサ
ンプル液Ｓａの殆どが無駄になることがある。In addition, in the second conventional example, the sample liquid Sa is sucked, passes through the three-way valve 10, is stored in the sample reservoir 11, and is then sent to the flow cell chamber 3, which is disadvantageous in terms of time. There is also a large possibility of so-called contamination, in which residual liquid from the previous measurement sample gets mixed in when the sample liquid Sa is poured. Further, since the amount of sample liquid Sa to be aspirated is fairly constant, depending on the concentration of sample liquid Sa, the desired number of samples to be measured may not be reached, or conversely, most of sample liquid Sa may be wasted.
本発明の目的は、コンタミネーションの虞れがなく能率
の良い測定が可能なフローセル装置を提供することにあ
る。An object of the present invention is to provide a flow cell device that can perform efficient measurements without the risk of contamination.
［課題を解決するための手段］
上述の目的を達成するために、本発明に係るフローセル
装置においては、フローセルチャンバ内にシース液とサ
ンプル液を供給し、サンプル液の周囲にシース液を流し
てシースフローを形成するフローセル装置において、廃
液を吸引する送液手段と、管路の途中に管路抵抗を変え
るための抵抗手段を設け前記フローセルチャンバ内にシ
ース液を導くシース液管と、一端をサンプル液中に浸漬
し他端を前記フローセルチャンバに接続したサンプル液
管とを有することを特徴とするものである。[Means for Solving the Problems] In order to achieve the above-mentioned object, in the flow cell device according to the present invention, a sheath liquid and a sample liquid are supplied into a flow cell chamber, and the sheath liquid is caused to flow around the sample liquid. In a flow cell device that forms a sheath flow, a sheath liquid pipe is provided with a liquid feeding means for sucking waste liquid and a resistance means for changing the resistance of the pipe line in the middle of the pipe line, and one end of the sheath liquid pipe is provided to guide the sheath liquid into the flow cell chamber. The sample liquid tube is immersed in the sample liquid and has its other end connected to the flow cell chamber.
［作用］
上述の構成を有するフローセル装置は、フロセルの下流
に送液手段を設けることにより、サンプル液を無駄にす
ることなく連続的な測定を可能とする。[Operation] The flow cell device having the above-described configuration enables continuous measurement without wasting sample liquid by providing a liquid feeding means downstream of the flow cell.
［実施例］
本発明を第１図〜第３図に図示の実施例に基づいて詳細
に説明する。[Example] The present invention will be explained in detail based on the example illustrated in FIGS. 1 to 3.
第１図は本発明の実施例を示し、第４図、第５図と同一
の符号は同一の部材を表している。フローセルチャンバ
３には、サンプル液容器ｌ内のサンプル液Ｓａを供給す
るサンプルチューブ２０と、シース液容器２内のシース
液ｓｈを供給するシースチューブ２１、及びサンプルチ
ューブ２０内を洗浄するための洗浄液Ｓｃを供給する洗
浄チューブ２２が接続されている。シースチューブ２１
の途中にはバルブ２３、可変の管路抵抗器２４が接続さ
れてξす、洗浄チューブ２２にはフローセルチャンバ３
に洗浄液Ｓｃを供給するための洗浄液供給機構２５が接
続されている。フローセル４の下流側には廃液チューブ
２６が接続されており、この廃液チューブ２６は廃液を
吸引する送液機構２７に接続されている。なお、２８は
サンプルチューブ２０のサンプル吸引部の外側を洗浄す
る洗浄液Ｓｃを供給するための洗浄機構である。FIG. 1 shows an embodiment of the present invention, and the same reference numerals as in FIGS. 4 and 5 represent the same members. The flow cell chamber 3 includes a sample tube 20 for supplying the sample liquid Sa in the sample liquid container l, a sheath tube 21 for supplying the sheath liquid sh in the sheath liquid container 2, and a cleaning liquid for cleaning the inside of the sample tube 20. A cleaning tube 22 that supplies Sc is connected. sheath tube 21
A valve 23 and a variable line resistor 24 are connected to the middle of the cleaning tube 22, and a flow cell chamber 3 is connected to the cleaning tube 22.
A cleaning liquid supply mechanism 25 for supplying cleaning liquid Sc is connected to the cleaning liquid Sc. A waste liquid tube 26 is connected to the downstream side of the flow cell 4, and this waste liquid tube 26 is connected to a liquid sending mechanism 27 that sucks the waste liquid. Note that 28 is a cleaning mechanism for supplying a cleaning liquid Sc for cleaning the outside of the sample suction part of the sample tube 20.
サンプル液Ｓａが入ったサンプル液容器１は、サンプル
チューブ２０のサンプル吸引部がサンプル液りａ内に浸
漬するよう置かれている。また、シースチューブ２１の
一端はシース液容器２内のシース液ｓｈ内に浸漬されて
いる。いま、送液機構２７を作動させるとシース液ｓｈ
及びサンプル液Ｓａは吸引され、フローセルチャンバ３
へと導かれる。送液機構２７としては、容器内部を真空
にすることにより廃液を吸引するようなものも考えられ
るが、廃液の流量を制御できる容積型ポンプの方が好ま
しいが、モータ駆動のシリンダなども考えられる。The sample liquid container 1 containing the sample liquid Sa is placed so that the sample suction part of the sample tube 20 is immersed in the sample liquid a. Further, one end of the sheath tube 21 is immersed in the sheath liquid sh in the sheath liquid container 2. Now, when the liquid feeding mechanism 27 is activated, the sheath liquid sh
and the sample liquid Sa are sucked into the flow cell chamber 3.
be led to. As the liquid feeding mechanism 27, a mechanism that suctions the waste liquid by creating a vacuum inside the container can be considered, but a positive displacement pump that can control the flow rate of the waste liquid is preferable, but a motor-driven cylinder can also be considered. .
第２図に示すようにフローセルチャンバ３内では、サン
プル液Ｓａの周囲をシース液ｓｈが流れ、フローセル４
内でシースフローが形成されている。As shown in FIG. 2, in the flow cell chamber 3, the sheath liquid sh flows around the sample liquid Sa, and the flow cell 4
A sheath flow is formed within.
流れの速度は送液機構２７の流量を変えることにより変
えられる。フローセルチャンバ３内は負圧になっており
、シース液ｓｈの流れが支配的であり、そのため管路抵
抗器２４の抵抗を少し大きくするとフローセルチャンバ
３内は更に負圧になり、その結果、サンプル液Ｓａの流
量が増えシースフローの流径が太くなる。逆に、管路抵
抗器２４の抵抗を少し小さくすると流径は細くなり、管
路抵抗器２４は流径を制御することになる。The speed of the flow can be changed by changing the flow rate of the liquid feeding mechanism 27. The inside of the flow cell chamber 3 is under negative pressure, and the flow of the sheath liquid sh is dominant. Therefore, if the resistance of the conduit resistor 24 is slightly increased, the inside of the flow cell chamber 3 becomes even more negative pressure, and as a result, the sample The flow rate of liquid Sa increases and the diameter of the sheath flow increases. Conversely, if the resistance of the conduit resistor 24 is slightly reduced, the flow diameter becomes narrower, and the conduit resistor 24 controls the flow diameter.
管路抵抗器２４としては、第３図に電気回路で等測的に
示すように内部に切換バルブと幾種類かの分岐路を持ち
、バルブによってシース液ｓｈの分岐路を切り換えるこ
とにより管路抵抗を変えるものが考えられる。管路抵抗
をシースフロー形成時の大きさとすると、送液機構２７
を始動した際にサンプル液Ｓａは吸引されるが、直ちに
はフローセルチャンバ３内に達しないので、始動時は管
路抵抗器２４の管路抵抗はシースフロー形成時の太きさ
よりも十分大きくするように切換えられており、サンプ
ル液Ｓａがフローセルチャンバ３内へ達した後に、所望
の流径に見当った抵抗値に切換えられる。The conduit resistor 24 has an internal switching valve and several types of branch paths, as shown isometrically in the electric circuit in FIG. One possibility is to change the resistance. If the pipe resistance is the magnitude at the time of sheath flow formation, the liquid feeding mechanism 27
When the sample liquid Sa is started, it is sucked in, but it does not reach the inside of the flow cell chamber 3 immediately. Therefore, at the time of starting, the pipe resistance of the pipe resistor 24 should be sufficiently larger than the thickness at the time of forming the sheath flow. After the sample liquid Sa reaches the inside of the flow cell chamber 3, the resistance value is changed to match the desired flow diameter.
フローセル４内ではレーザー光による測定が行われ、測
定が終わるとサンプル液容器１を取り除き、サンプルチ
ューブ２０の個所に洗浄機構２８を持つでくる。次に、
バルブ２３を閉じると同時に、洗浄液供給機構２５を駆
動し送液機構２７よりも多い流量で洗浄液Ｓｃをフロー
セルチャンバ３に送る。すると、洗浄液供給機構２５か
らの洗浄液Ｓｃはフローセルチャンバ３、フローセル４
、廃液チューブ２６を経由して流れ、洗浄と同時にサン
プルチューブ２０内を逆流し洗浄を行う。このとき、同
時に洗浄機構２８によりサンプルチｘ −ブ２０のサン
プル吸引部の外側を洗浄する。この洗浄によってサンプ
ルチューブ２０内に残ったサンプル液Ｓａは捨てられる
が、例えば内径０．５ｍｍ、長さ１５ｍｍのサンプルチ
ューブを使用すると、その廃棄量は約３ｕ！２と少ない
。Inside the flow cell 4, measurement is performed using a laser beam, and when the measurement is finished, the sample liquid container 1 is removed and a cleaning mechanism 28 is placed in place of the sample tube 20. next,
At the same time as closing the valve 23, the cleaning liquid supply mechanism 25 is driven to send the cleaning liquid Sc to the flow cell chamber 3 at a flow rate higher than that of the liquid feeding mechanism 27. Then, the cleaning liquid Sc from the cleaning liquid supply mechanism 25 is supplied to the flow cell chamber 3 and the flow cell 4.
, flows through the waste liquid tube 26, and at the same time as cleaning, backflows inside the sample tube 20 to perform cleaning. At this time, the cleaning mechanism 28 simultaneously cleans the outside of the sample suction section of the sample tube 20. By this washing, the sample liquid Sa remaining in the sample tube 20 is discarded, but if a sample tube with an inner diameter of 0.5 mm and a length of 15 mm is used, the amount of waste is approximately 3 u! As few as 2.
［発明の効果］
以上説明したように本発明に係るフローセル装置は、ｆ
ｉ＋装置の構成が簡単である、（２）サンプル液を短時
間でフローセルまで導ける、（３）サンプルチューブが
１本の管であるのでコンタミネーションの心配が殆どな
い、（４）測定を任意の時間内で終えることができサン
プル液を無駄にしない、（５）自動的に多検体を処理す
る装置に応用できるなどの効果がある。[Effect of the invention] As explained above, the flow cell device according to the present invention has f
The configuration of the i+ device is simple, (2) the sample liquid can be guided to the flow cell in a short time, (3) there is almost no concern about contamination because the sample tube is a single tube, and (4) measurements can be performed at any time. It has the following advantages: it can be completed within a certain amount of time, no sample liquid is wasted, and (5) it can be applied to devices that automatically process multiple samples.
図面第１図〜第３図は本発明に係るフローセル装置の実
施例を示し、第１図は構成図、第２図はフローセルチャ
ンバの断面図、第３図は管路抵抗器の等測面であり、第
４図は第１の従来例の構成図、第５図は第２の従来例の
構成図である。
符号１はサンプル液容器、２はシース液容器、３はフロ
ーセルチャンバ、４はフローセル、２０はサンプルチュ
ーブ、２１はシースチューブ、２３はバルブ、２４は管
路抵抗器、２２は洗浄チューブ、２５は洗浄液供給機構
、２６は廃液チューブ、
２７は送液機構、
２８は洗浄機構、
ａ
はサンプル液、
ｓｈはシ
ス液。
Ｓｃは洗浄液であ
る。Drawings 1 to 3 show an embodiment of the flow cell device according to the present invention, in which Fig. 1 is a configuration diagram, Fig. 2 is a sectional view of the flow cell chamber, and Fig. 3 is an isometric surface of a conduit resistor. FIG. 4 is a block diagram of the first conventional example, and FIG. 5 is a block diagram of the second conventional example. 1 is a sample liquid container, 2 is a sheath liquid container, 3 is a flow cell chamber, 4 is a flow cell, 20 is a sample tube, 21 is a sheath tube, 23 is a valve, 24 is a line resistor, 22 is a cleaning tube, 25 is a 26 is a cleaning liquid supply mechanism, 26 is a waste liquid tube, 27 is a liquid sending mechanism, 28 is a cleaning mechanism, a is a sample liquid, and sh is a cis liquid. Sc is a cleaning liquid.
Claims (1)
給し、サンプル液の周囲にシース液を流してシースフロ
ーを形成するフローセル装置において、廃液を吸引する
送液手段と、管路の途中に管路抵抗を変えるための抵抗
手段を設け前記フローセルチャンバ内にシース液を導く
シース液管と、一端をサンプル液中に浸漬し他端を前記
フローセルチャンバに接続したサンプル液管とを有する
ことを特徴とするフローセル装置。1. In a flow cell device that supplies a sheath liquid and a sample liquid into a flow cell chamber and flows the sheath liquid around the sample liquid to form a sheath flow, there is a liquid feeding means for sucking the waste liquid, and a pipe line in the middle of the pipe line. It is characterized by having a sheath liquid tube that is provided with resistance means for changing the resistance and guides the sheath liquid into the flow cell chamber, and a sample liquid tube whose one end is immersed in the sample liquid and the other end is connected to the flow cell chamber. flow cell device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2143458A JPH0436636A (en) | 1990-06-01 | 1990-06-01 | Flow cell device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2143458A JPH0436636A (en) | 1990-06-01 | 1990-06-01 | Flow cell device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0436636A true JPH0436636A (en) | 1992-02-06 |
Family
ID=15339175
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2143458A Pending JPH0436636A (en) | 1990-06-01 | 1990-06-01 | Flow cell device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0436636A (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0602416A1 (en) * | 1992-12-14 | 1994-06-22 | Becton, Dickinson and Company | Method for control of flow cytometer having vacuum driven flow |
| WO1999046047A2 (en) * | 1998-03-10 | 1999-09-16 | Large Scale Proteomics Corporation | Detection and characterization of microorganisms |
| WO2009119592A1 (en) | 2008-03-25 | 2009-10-01 | 旭化成ケミカルズ株式会社 | Elastomer composition and storage cover for airbag system |
| WO2009128397A1 (en) | 2008-04-14 | 2009-10-22 | 旭化成ケミカルズ株式会社 | Adhesive composition |
| WO2010067564A1 (en) | 2008-12-10 | 2010-06-17 | 旭化成ケミカルズ株式会社 | Thermoplastic elastomer composition |
| WO2010073589A1 (en) | 2008-12-22 | 2010-07-01 | 旭化成ケミカルズ株式会社 | Crosslinkable and foamable composition, crosslinked foamed object, and shoe midsole comprising same |
| WO2011040312A1 (en) | 2009-10-02 | 2011-04-07 | 旭化成ケミカルズ株式会社 | Production method for modified conjugated diene polymer, modified conjugated diene polymer, and modified conjugated diene polymer composition |
| WO2011111696A1 (en) | 2010-03-08 | 2011-09-15 | 旭化成ケミカルズ株式会社 | Foam composition, method for producing same, and foam |
| CN104769414A (en) * | 2012-09-06 | 2015-07-08 | 古河电气工业株式会社 | Device for identifying and dispensing samples and method for identifying and dispensing samples |
| EP4265646A1 (en) | 2022-04-18 | 2023-10-25 | Japan Elastomer Co., Ltd. | Crumb of block copolymer or hydrogenated product thereof and aspahlt composition |
-
1990
- 1990-06-01 JP JP2143458A patent/JPH0436636A/en active Pending
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0602416A1 (en) * | 1992-12-14 | 1994-06-22 | Becton, Dickinson and Company | Method for control of flow cytometer having vacuum driven flow |
| US5395588A (en) * | 1992-12-14 | 1995-03-07 | Becton Dickinson And Company | Control of flow cytometer having vacuum fluidics |
| WO1999046047A2 (en) * | 1998-03-10 | 1999-09-16 | Large Scale Proteomics Corporation | Detection and characterization of microorganisms |
| WO1999046047A3 (en) * | 1998-03-10 | 1999-12-02 | Biosource Proteomics Inc | Detection and characterization of microorganisms |
| US6254834B1 (en) | 1998-03-10 | 2001-07-03 | Large Scale Proteomics Corp. | Detection and characterization of microorganisms |
| US6340570B1 (en) | 1998-03-10 | 2002-01-22 | Large Scale Proteomics Corp. | Detection and characterization of microorganisms |
| US6346421B1 (en) | 1998-03-10 | 2002-02-12 | Large Scale Proteomics Corp. | Methods for concentrating and detecting microorganisms using centrifuge tubes |
| US6479239B1 (en) | 1998-03-10 | 2002-11-12 | Large Scale Biology Corporation | Detection and characterization of microorganisms |
| US6911312B2 (en) | 1998-03-10 | 2005-06-28 | Large Scale Proteomics Corporation | Detection and characterization of microorganisms |
| US7070739B1 (en) | 1998-03-10 | 2006-07-04 | Large Scale Proteomics Corporation | Detection and characterization of microorganisms |
| WO2009119592A1 (en) | 2008-03-25 | 2009-10-01 | 旭化成ケミカルズ株式会社 | Elastomer composition and storage cover for airbag system |
| WO2009128397A1 (en) | 2008-04-14 | 2009-10-22 | 旭化成ケミカルズ株式会社 | Adhesive composition |
| WO2010067564A1 (en) | 2008-12-10 | 2010-06-17 | 旭化成ケミカルズ株式会社 | Thermoplastic elastomer composition |
| WO2010073589A1 (en) | 2008-12-22 | 2010-07-01 | 旭化成ケミカルズ株式会社 | Crosslinkable and foamable composition, crosslinked foamed object, and shoe midsole comprising same |
| WO2011040312A1 (en) | 2009-10-02 | 2011-04-07 | 旭化成ケミカルズ株式会社 | Production method for modified conjugated diene polymer, modified conjugated diene polymer, and modified conjugated diene polymer composition |
| WO2011111696A1 (en) | 2010-03-08 | 2011-09-15 | 旭化成ケミカルズ株式会社 | Foam composition, method for producing same, and foam |
| CN104769414A (en) * | 2012-09-06 | 2015-07-08 | 古河电气工业株式会社 | Device for identifying and dispensing samples and method for identifying and dispensing samples |
| EP4265646A1 (en) | 2022-04-18 | 2023-10-25 | Japan Elastomer Co., Ltd. | Crumb of block copolymer or hydrogenated product thereof and aspahlt composition |
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