JPH04297402A - Agent for decreasing oxalic acid content of vegetable and decreasing method - Google Patents
Agent for decreasing oxalic acid content of vegetable and decreasing methodInfo
- Publication number
- JPH04297402A JPH04297402A JP13370991A JP13370991A JPH04297402A JP H04297402 A JPH04297402 A JP H04297402A JP 13370991 A JP13370991 A JP 13370991A JP 13370991 A JP13370991 A JP 13370991A JP H04297402 A JPH04297402 A JP H04297402A
- Authority
- JP
- Japan
- Prior art keywords
- oxalic acid
- decreasing
- acid content
- agent
- plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 title claims abstract description 69
- 235000006408 oxalic acid Nutrition 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 8
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 6
- 230000003247 decreasing effect Effects 0.000 title abstract 6
- 235000013311 vegetables Nutrition 0.000 title 1
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 claims abstract description 26
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 claims abstract description 13
- 229940011671 vitamin b6 Drugs 0.000 claims abstract description 13
- 239000011726 vitamin B6 Substances 0.000 claims abstract description 11
- 235000019158 vitamin B6 Nutrition 0.000 claims abstract description 11
- 150000003839 salts Chemical class 0.000 claims abstract description 10
- 239000004480 active ingredient Substances 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 abstract 2
- 241000196324 Embryophyta Species 0.000 description 11
- 241000219315 Spinacia Species 0.000 description 7
- 235000009337 Spinacia oleracea Nutrition 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 239000003638 chemical reducing agent Substances 0.000 description 4
- 239000011575 calcium Substances 0.000 description 3
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- 229930191978 Gibberellin Natural products 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- 239000000538 analytical sample Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 159000000007 calcium salts Chemical class 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 239000003448 gibberellin Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- NHZMQXZHNVQTQA-UHFFFAOYSA-N pyridoxamine Chemical compound CC1=NC=C(CO)C(CN)=C1O NHZMQXZHNVQTQA-UHFFFAOYSA-N 0.000 description 2
- 235000008160 pyridoxine Nutrition 0.000 description 2
- 239000011677 pyridoxine Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 229960003581 pyridoxal Drugs 0.000 description 1
- 235000008164 pyridoxal Nutrition 0.000 description 1
- 239000011674 pyridoxal Substances 0.000 description 1
- 235000008151 pyridoxamine Nutrition 0.000 description 1
- 239000011699 pyridoxamine Substances 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 201000009160 urethral calculus Diseases 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Abstract
Description
【０００１】0001
【産業上の利用分野】本発明は、植物に蓄積されるシュ
ウ酸含量の軽減に関するものであり、詳しくは、ビタミ
ンＢ６群および／またはその塩を有効成分とする植物の
シュウ酸含量の軽減剤を提供するものである。[Field of Industrial Application] The present invention relates to reducing the oxalic acid content accumulated in plants, and more specifically, an agent for reducing the oxalic acid content of plants containing vitamin B6 group and/or its salt as an active ingredient. It provides:
【０００２】0002
【背景技術】従来よりシュウ酸は硝酸塩とともに、植物
中に含まれる天然有害成分（食の科学，３８，５，１９
７７）として良く知られているが、我が国においても、
多食するホウレンソウ中の存在は古くから著明である。[Background technology] Oxalic acid has traditionally been a natural harmful component contained in plants (Food Science, 38, 5, 19) together with nitrate.
77), but in Japan as well,
Its presence in spinach, which is eaten extensively, has been evident for a long time.
【０００３】シュウ酸は植物体においては不溶性のカリ
ウム塩およびカルシウム塩として存在し、体内において
はカルシウム塩を形成し、尿道結石の一因と言われてい
る（生物学辞典，第２版，岩波書店，５１２，１９７７
）。こうしたヒトや動物における安全性の立場から、こ
れら植物体のシュウ酸含量を目安とした品質保全および
収量につき栽培条件の改善によって向上しようとする研
究（岐阜女子大学紀要，１６，１３，１９８７）、ある
いは植物ホルモンのジベレリン処理による改善研究（山
口農試研報，４１，３２，１９８９）、更にはＤ−パン
トテン酸カルシウムによる研究等がなされているが、い
ずれも品質保全および収量の両者を同時に満足させるに
至っていない。特にジベレリンはその処理によりシュウ
酸含量を低下させることが出来るがその効果も弱く、又
収量にもつながらず、更には非常に高価であることから
未だ実用化に至ってない。[0003] Oxalic acid exists as insoluble potassium salts and calcium salts in plants, and forms calcium salts in the body, and is said to be a cause of urethral stones (Biological Dictionary, 2nd edition, Iwanami et al. Bookstore, 512, 1977
). From the standpoint of safety for humans and animals, research aimed at improving the quality and yield of these plants using the oxalic acid content as a guideline by improving cultivation conditions (Gifu Women's University Bulletin, 16, 13, 1987). Alternatively, improvement research has been carried out using the plant hormone gibberellin treatment (Yamaguchi Agricultural Research Report, 41, 32, 1989), and further research using calcium D-pantothenate, both of which satisfy both quality preservation and yield at the same time. has not yet been reached. In particular, gibberellins can reduce the oxalic acid content by treatment, but this effect is weak, does not lead to good yield, and is very expensive, so it has not yet been put to practical use.
【０００４】0004
【発明の開示】本発明者らは、植物に蓄積されるシュウ
酸含量の軽減方法につき、鋭意研究を行った結果、本発
明により優れた軽減剤を提供することに成功した。即ち
、本発明は、ビタミンＢ６群および／またはその塩を有
効成分とする軽減剤およびビタミンＢ６群および／また
はその塩を水に溶解せしめ、植物に適用することにより
、植物のシュウ酸の含量を軽減する方法を提供するもの
である。以下に本発明を詳細に説明する。DISCLOSURE OF THE INVENTION The present inventors have conducted intensive research on methods for reducing the oxalic acid content accumulated in plants, and as a result of this invention, they have succeeded in providing an excellent reducing agent. That is, the present invention reduces the oxalic acid content of plants by dissolving a reducing agent containing vitamin B6 group and/or its salt as an active ingredient, and dissolving the vitamin B6 group and/or its salt in water and applying it to the plant. This provides a method to reduce the The present invention will be explained in detail below.
【０００５】本発明者らは、適用植物としてホウレンソ
ウを選択し、土壌として第３紀層風化の丘陵未耕地の下
層土を使用し、ピリドキシン（以下Ｖ−Ｂ６と記す）を
用いて下記の要領により、実験を行った。
１．Ｖ−Ｂ６の使用濃度は、０．１〜０．２％溶液とし
て用いる。
２．使用量は、１ｍ２当り１５０ｍｌを散布する（１０
ａ当り１５０ ｌ）。
３．使用時期は、収穫前数日〜２週間前に１回散布する
。The present inventors selected spinach as the applicable plant, used Tertiary layer weathered subsoil of uncultivated hilly land as the soil, and performed the following procedure using pyridoxine (hereinafter referred to as V-B6). An experiment was conducted. 1. The concentration of V-B6 used is 0.1 to 0.2% solution. 2. The usage amount is 150ml per 1m2 (10
150 l per a). 3. When to use, spray once a few days to two weeks before harvest.
【０００６】上記の実験の結果、ビタミンＢ６群および
／またはその塩は、下記の如き優れた性質を有すること
が認められた。
（１）Ｄ−パントテン酸カルシウム（以下Ｐ−Ｃａと記
す）を用いた場合に比べて、シュウ酸含量の軽減効果は
著しく優れている。
（２）作物の収量の減少を招くことなく使用できる。
（３）毒性も低く、安価である。
このようにビタミンＢ６群および／またはその塩は、植
物のシュウ酸含量の軽減剤として実用化に供しうる効果
が認められた初めての物質である。As a result of the above experiments, it was found that vitamin B6 group and/or its salts have the following excellent properties. (1) Compared to the case of using calcium D-pantothenate (hereinafter referred to as P-Ca), the effect of reducing the oxalic acid content is significantly superior. (2) It can be used without causing a decrease in crop yield. (3) It has low toxicity and is inexpensive. As described above, vitamin B6 group and/or its salts are the first substances that have been recognized to have a practical effect as a reducing agent for the oxalic acid content in plants.
【０００７】本発明に係るシュウ酸含量の軽減剤の有効
成分としては、ビタミンＢ６群のピリドキシン、ピリド
キサル、ピリドキサミンの他に塩酸塩、硝酸塩などの水
溶性塩が用いられる。また薬剤組成物としては、溶剤と
して、例えば、水もしくは水に適当なほかの補助剤を溶
解せしめたものを使用し、これに通常０．１％程度の濃
度をもって上記有効成分物質を溶解せしめたものを用い
るが、これは、使用直前に溶液を調製することができる
ような状態で保管しておいてもよい。As active ingredients of the oxalic acid content reducing agent according to the present invention, in addition to pyridoxine, pyridoxal, and pyridoxamine of the vitamin B6 group, water-soluble salts such as hydrochloride and nitrate are used. In addition, for pharmaceutical compositions, the solvent used is, for example, water or water dissolved with other suitable adjuvants, and the above-mentioned active ingredient is dissolved in this at a concentration of usually about 0.1%. This may be stored in such a way that the solution can be prepared immediately before use.
【０００８】以下に、本発明の実施例および参考例を掲
げ、本発明をさらに具体的に説明するが、本発明は実施
例により限定されるものではない。[0008] The present invention will be described in more detail below with reference to Examples and Reference Examples, but the present invention is not limited by the Examples.
【０００９】[0009]
【実施例】材料と方法；ホウレンソウの供試品種として
は、グローリーホウレンソウ（秋作）を用いた。栽培は
、１／５０００アールポットに播種し、生育と共に漸次
間引きし、均一な３個体を残し成育させた。なお、１検
体当り７ポット（ｎ＝７）を用いた。供試土壌は、第３
紀層風化の丘陵未耕地の下層土を用い、肥料は化成肥料
でポット当りＮ−Ｐ２Ｏ５−Ｋ２Ｏを各々１．５ｇ他、
マグロポン６．０ｇをそれぞれ混合し施用した。ビタミ
ンＢ６群の処理濃度としては０．１％および０．２％の
各水溶液を用いた。対照試験は水を用いて行い、薬量は
ポット当り１５０ｍｌを茎葉散布した。収穫は薬剤処理
後１０日目に行った。[Example] Materials and Methods: Glory spinach (autumn crop) was used as a test variety of spinach. For cultivation, the seeds were sown in 1/5000 are pots, and as they grew, they were gradually thinned out, leaving three uniform individuals to grow. Note that 7 pots (n=7) were used for each sample. The test soil is No. 3
The subsoil of uncultivated hilly land with weathered layers was used, and the fertilizer was chemical fertilizer with 1.5g each of N-P2O5-K2O per pot, etc.
6.0 g of Maguropon was mixed and applied. As treatment concentrations of vitamin B6 group, 0.1% and 0.2% aqueous solutions were used. A control test was conducted using water, and the amount of the drug was 150 ml per pot, which was applied to the leaves and leaves. Harvesting was performed on the 10th day after chemical treatment.
【００１０】分析試料の調製および全シュウ酸含量の定
量；新鮮な収穫物の１０ｇを正確に秤量し、１Ｎ Ｈ
Ｃｌを２０ｍｌ加え、ホモジナイズする。ホモジネート
は、遠心分離で上澄液と沈殿物とに分け、沈殿物はさら
に２回１Ｎ ＨＣｌで洗浄する。上澄液と洗浄液とを
合わせ水で１００ｍｌとした。この抽出液を０．４５ｍ
μミリポアフィルターにより濾過した後、高速液体クロ
マトグラフィー（ＨＰＬＣ）により以下の条件でシュウ
酸の定量を行った。Preparation of analytical samples and determination of total oxalic acid content; Accurately weigh 10 g of fresh harvest and add 1N H
Add 20 ml of Cl and homogenize. The homogenate is separated into a supernatant and a precipitate by centrifugation, and the precipitate is further washed twice with 1N HCl. The supernatant liquid and washing liquid were combined and made up to 100 ml with water. 0.45m of this extract
After filtration with a μ Millipore filter, oxalic acid was quantified by high performance liquid chromatography (HPLC) under the following conditions.
【００１１】結果を第１表に示す。The results are shown in Table 1.
【００１２】0012
【表１】[Table 1]
【００１３】薬剤は１回処理とし、処理後１０日目で収
穫し分析した。[0013] The drug was treated once, and the plants were harvested and analyzed 10 days after the treatment.
【００１４】〔参考例〕材料および方法；ホウレンソウ
の供試品種としてはグローリーホウレンソウ（秋作）を
用いた。栽培は実施例１と全く同様の方法で行った。薬
剤散布はＤ−パントテン酸カルシウム（以下Ｐ−Ｃａと
記す）の０．１％および０．２％各水溶液を用いた。対
照試験は水を用いて行った。ポット当り１５０ｍｌの茎
葉散布し、収穫は薬剤処理後１０日目に行った。[Reference Example] Materials and Methods: Glory spinach (autumn crop) was used as a test variety of spinach. Cultivation was carried out in exactly the same manner as in Example 1. For chemical spraying, 0.1% and 0.2% aqueous solutions of calcium D-pantothenate (hereinafter referred to as P-Ca) were used. A control test was performed using water. 150 ml per pot was sprayed on the foliage, and harvested on the 10th day after the chemical treatment.
【００１５】分析試料の調製および全シュウ酸含量の定
量；実施例と全く同様の方法で行った。結果を第２表に
示す。Preparation of analytical samples and determination of total oxalic acid content; carried out in exactly the same manner as in the examples. The results are shown in Table 2.
【００１６】[0016]
【表２】[Table 2]
【００１７】以上の結果から明らかなようにビタミンＢ
６群はホウレンソウにおいて０．１％濃度で著しいシュ
ウ酸含量の軽減効果が認められ、又その収量も増加傾向
を認めた。一方、Ｐ−Ｃａはシュウ酸含量の軽減効果は
認めなかった。[0017] As is clear from the above results, vitamin B
In group 6, a remarkable effect of reducing the oxalic acid content was observed in spinach at a concentration of 0.1%, and the yield also tended to increase. On the other hand, no effect of reducing the oxalic acid content was observed in P-Ca.
Claims (2)
の塩を有効成分とする植物のシュウ酸含量の軽減剤。1. An agent for reducing the oxalic acid content of plants, which contains vitamin B6 group and/or its salt as an active ingredient.
の塩の溶液を、植物に適用することを特徴とする植物の
シュウ酸含量を軽減させる方法。2. A method for reducing the oxalic acid content of plants, which comprises applying a solution of vitamin B6 group and/or its salts to the plants.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3133709A JP3029699B2 (en) | 1991-03-26 | 1991-03-26 | Agent and method for reducing oxalic acid content in leafy vegetables |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3133709A JP3029699B2 (en) | 1991-03-26 | 1991-03-26 | Agent and method for reducing oxalic acid content in leafy vegetables |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04297402A true JPH04297402A (en) | 1992-10-21 |
| JP3029699B2 JP3029699B2 (en) | 2000-04-04 |
Family
ID=15111062
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3133709A Expired - Lifetime JP3029699B2 (en) | 1991-03-26 | 1991-03-26 | Agent and method for reducing oxalic acid content in leafy vegetables |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3029699B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0639329B1 (en) * | 1993-08-19 | 1997-03-05 | Cosmo Research Institute | Methods for reducing oxalic acid contents in plants |
-
1991
- 1991-03-26 JP JP3133709A patent/JP3029699B2/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0639329B1 (en) * | 1993-08-19 | 1997-03-05 | Cosmo Research Institute | Methods for reducing oxalic acid contents in plants |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3029699B2 (en) | 2000-04-04 |
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