JPH0428719B2 - - Google Patents
Info
- Publication number
- JPH0428719B2 JPH0428719B2 JP56172910A JP17291081A JPH0428719B2 JP H0428719 B2 JPH0428719 B2 JP H0428719B2 JP 56172910 A JP56172910 A JP 56172910A JP 17291081 A JP17291081 A JP 17291081A JP H0428719 B2 JPH0428719 B2 JP H0428719B2
- Authority
- JP
- Japan
- Prior art keywords
- protein
- avidin
- chain
- complex
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 108090000623 proteins and genes Proteins 0.000 claims description 105
- 108090001008 Avidin Proteins 0.000 claims description 65
- 230000014616 translation Effects 0.000 claims description 42
- 230000002401 inhibitory effect Effects 0.000 claims description 40
- 239000012634 fragment Substances 0.000 claims description 21
- 125000003396 thiol group Chemical group [H]S* 0.000 claims description 21
- 125000002228 disulfide group Chemical group 0.000 claims description 20
- 238000004132 cross linking Methods 0.000 claims description 17
- 108010053187 Diphtheria Toxin Proteins 0.000 claims description 12
- 102000016607 Diphtheria Toxin Human genes 0.000 claims description 12
- 239000004472 Lysine Substances 0.000 claims description 8
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 7
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- 108010039491 Ricin Proteins 0.000 description 25
- 238000000034 method Methods 0.000 description 24
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 21
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- JWDFQMWEFLOOED-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 3-(pyridin-2-yldisulfanyl)propanoate Chemical compound O=C1CCC(=O)N1OC(=O)CCSSC1=CC=CC=N1 JWDFQMWEFLOOED-UHFFFAOYSA-N 0.000 description 4
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 4
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- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
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- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
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- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- XTHFKEDIFFGKHM-UHFFFAOYSA-N Dimethoxyethane Chemical compound COCCOC XTHFKEDIFFGKHM-UHFFFAOYSA-N 0.000 description 2
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- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
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- MUKYLHIZBOASDM-UHFFFAOYSA-N 2-[carbamimidoyl(methyl)amino]acetic acid 2,3,4,5,6-pentahydroxyhexanoic acid Chemical compound NC(=N)N(C)CC(O)=O.OCC(O)C(O)C(O)C(O)C(O)=O MUKYLHIZBOASDM-UHFFFAOYSA-N 0.000 description 1
- 125000004105 2-pyridyl group Chemical group N1=C([*])C([H])=C([H])C([H])=C1[H] 0.000 description 1
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- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- HAEPBEMBOAIUPN-UHFFFAOYSA-L sodium tetrathionate Chemical compound O.O.[Na+].[Na+].[O-]S(=O)(=O)SSS([O-])(=O)=O HAEPBEMBOAIUPN-UHFFFAOYSA-L 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000003573 thiols Chemical group 0.000 description 1
- DVKJHBMWWAPEIU-UHFFFAOYSA-N toluene 2,4-diisocyanate Chemical compound CC1=CC=C(N=C=O)C=C1N=C=O DVKJHBMWWAPEIU-UHFFFAOYSA-N 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 229940047183 tribulus Drugs 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 239000002753 trypsin inhibitor Substances 0.000 description 1
- 239000011735 vitamin B7 Substances 0.000 description 1
- 235000011912 vitamin B7 Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56172910A JPS5874614A (ja) | 1981-10-30 | 1981-10-30 | 蛋白複合体及びその製造法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56172910A JPS5874614A (ja) | 1981-10-30 | 1981-10-30 | 蛋白複合体及びその製造法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5874614A JPS5874614A (ja) | 1983-05-06 |
| JPH0428719B2 true JPH0428719B2 (fr) | 1992-05-15 |
Family
ID=15950606
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56172910A Granted JPS5874614A (ja) | 1981-10-30 | 1981-10-30 | 蛋白複合体及びその製造法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5874614A (fr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4664911A (en) * | 1983-06-21 | 1987-05-12 | Board Of Regents, University Of Texas System | Immunotoxin conjugates employing toxin B chain moieties |
| JPS6028997A (ja) * | 1983-07-26 | 1985-02-14 | Sumitomo Chem Co Ltd | 新規なポリペプチドおよびその製造法 |
| JP2646048B2 (ja) * | 1991-10-24 | 1997-08-25 | キユーピー 株式会社 | リゾチーム含有医薬組成物 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5549321A (en) * | 1978-09-28 | 1980-04-09 | Clin Midy | Cellular toxin product formed by covalent bond of lysine a chain with antibody |
-
1981
- 1981-10-30 JP JP56172910A patent/JPS5874614A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5549321A (en) * | 1978-09-28 | 1980-04-09 | Clin Midy | Cellular toxin product formed by covalent bond of lysine a chain with antibody |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5874614A (ja) | 1983-05-06 |
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