JPH0425768B2 - - Google Patents
Info
- Publication number
- JPH0425768B2 JPH0425768B2 JP61063701A JP6370186A JPH0425768B2 JP H0425768 B2 JPH0425768 B2 JP H0425768B2 JP 61063701 A JP61063701 A JP 61063701A JP 6370186 A JP6370186 A JP 6370186A JP H0425768 B2 JPH0425768 B2 JP H0425768B2
- Authority
- JP
- Japan
- Prior art keywords
- artemia
- culture solution
- per
- ppm
- feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000238582 Artemia Species 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 11
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 7
- 229910052760 oxygen Inorganic materials 0.000 claims description 7
- 239000001301 oxygen Substances 0.000 claims description 7
- 238000005273 aeration Methods 0.000 claims description 3
- 238000007664 blowing Methods 0.000 claims 1
- 239000000243 solution Substances 0.000 description 11
- 235000013601 eggs Nutrition 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 6
- 239000013535 sea water Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000012447 hatching Effects 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000206761 Bacillariophyta Species 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 239000004419 Panlite Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 241000238426 Anostraca Species 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 241000239250 Copepoda Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000133262 Nauplius Species 0.000 description 1
- 241000195659 Neodesmus pupukensis Species 0.000 description 1
- 241000269908 Platichthys flesus Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Description
【発明の詳細な説明】
(a) 産業上の利用分野
本発明は、魚類および甲殻類の種苗生産に用い
られるアルテミアを高密度で効率良く養成する方
法に関する。DETAILED DESCRIPTION OF THE INVENTION (a) Field of Industrial Application The present invention relates to a method for efficiently cultivating Artemia at high density for use in producing seedlings of fish and crustaceans.
(b) 従来の技術
アルテミア（ブラインシユリンプ）は、ヒラメ
をはじめとする各種海産魚や、車エビの稚仔養成
用生物餌料として広く利用されている。(b) Conventional technology Artemia (brine shrimp) is widely used as a biological feed for raising the fry of various marine fish such as flounder and shrimp.
現在、アルテミアの耐久卵の殆ど全ては、孵化
直後のノープリウス期（体長0.5mm前後）に、そ
のままあるいは油脂酵母などで栄養強化して生物
餌料として投与されている。投与時期は、シオミ
ズツボワシムで飼育した後であるが、現状ではノ
ープリウスで投与する為、投与期間が短い。さら
にアルテミアノープリウスの次には、天然のアル
チアやコペポーダーなどが投与されるが、このよ
うな天然プランクトンは計画的に採集する事が困
難である為、耐久卵が容易に入手できるアルテミ
アを養成することに対する要望が強かつた。そこ
で、酵母エキス、鶏糞、人工飼料（商品名：マリ
ンメイト）などを使い天然海水中に存在するバク
テリアやケイ藻を増殖させ、これを餌料として消
化管が未成熟なアルテミアノープリウスを養成す
る方法が開発され、いくつかの種苗生産施設で成
功している例もあるが、生産されるバクテリアや
ケイ藻の量および通気量が限られている為、培養
液１ml当り１個体／ml前後でしか養成されていな
かつた。 Currently, almost all of Artemia's durable eggs are fed as biological feed during the nauplius stage (body length of around 0.5 mm) immediately after hatching, either as they are or fortified with oleaginous yeast. The timing of administration is after the animals have been reared on Shiomitsuboisim, but as it is currently administered on nauplii, the administration period is short. Furthermore, next to Artemia nauplii, natural Artemia and Copepoda are administered, but since it is difficult to systematically collect such natural plankton, Artemia, whose durable eggs are easily available, is cultivated. There was a strong demand for this. Therefore, we use yeast extract, chicken manure, artificial feed (product name: Marine Mate), etc. to grow bacteria and diatoms that exist in natural seawater, and use this as food to cultivate Artemia nauplii, which have immature digestive tracts. has been developed and has been successful in some seed production facilities, but because the amount of bacteria and diatoms produced and the amount of aeration are limited, only about 1 individual/ml per ml of culture solution. I wasn't trained.
本発明者らは、先にアルテミア用飼料として消
化率の悪い海産藻類を可消化処理したものを開発
し、これにより定常的にアルテミアの養成が可能
になつた（特願昭60−227977、特願昭60−255979
号）。そこで効率良くアルテミアを養成するため
には培養液の単位体積当りのアルテミア個体数を
増加させる事が考えられるようになつたが、高密
度養成およびそれにともなう給飼量の増加で、水
質の悪化、溶存酸素量の低下が生じ、養成過程に
おけるアルテミアの死亡率が高かつた。 The present inventors have previously developed a feed for Artemia that is made from poorly digestible marine algae, which has made it possible to regularly cultivate Artemia (Japanese Patent Application No. 60-227977, Gansho 60-255979
issue). Therefore, in order to cultivate Artemia efficiently, it has been considered to increase the number of Artemia individuals per unit volume of culture solution, but high-density cultivation and the accompanying increase in feeding amount lead to deterioration of water quality and The amount of dissolved oxygen decreased, and the mortality rate of Artemia during the cultivation process was high.
(c) 発明が解決しようとする問題点
本発明の目的は、アルテミアノープリウスの消
化可能な飼料を用いて養成を行う場合、その過程
におけるアルテミアの死亡率を低くする高密度培
養方法を提供することにある。(c) Problems to be Solved by the Invention The purpose of the present invention is to provide a high-density culture method that reduces the mortality rate of Artemia nauplii during the cultivation process using digestible feed. There is a particular thing.
(d) 問題点を解決するための手段
本発明者らは、鋭意研究の結果、アルテミア培
養液（天然または人工海水）中の溶存酸素濃度
（以下、D.O.という）を3ppm以上の範囲に維持
することにより高密度（好ましくは培養液１ml当
り30〜50固体）で、２〜３mmサイズまでのアルテ
ミアの養成が達成できることを見出した。即ち、
本発明は培養液中のD.O.を3ppm以上に維持する
ことを特徴とするアルテミアの高濃度養成方法で
ある。(d) Means for solving the problem As a result of intensive research, the present inventors have determined to maintain the dissolved oxygen concentration (hereinafter referred to as DO) in the Artemia culture solution (natural or artificial seawater) within a range of 3 ppm or more. It has been found that by this method, cultivation of Artemia up to a size of 2 to 3 mm can be achieved at high density (preferably 30 to 50 solids per ml of culture solution). That is,
The present invention is a method for cultivating Artemia at a high concentration, which is characterized by maintaining DO in the culture solution at 3 ppm or more.
D.O.を3ppm以上に維持する方法としては、次
の手段がある。 The following methods can be used to maintain DO at 3ppm or higher.
コンプレツサー等により培養液１トンに対し
て、毎分10を下限として圧縮空気の通気を行
う。 Aerate compressed air per ton of culture solution using a compressor or the like at a minimum rate of 10 per minute.
気体状酸素を培養液１トン当り毎分100ml以
上吹き込む。このためには例えば圧縮酸素（酸
素濃度99.6％）などを用いることができる。 Gaseous oxygen is blown in at least 100ml per minute per ton of culture solution. For this purpose, for example, compressed oxygen (oxygen concentration 99.6%) can be used.
前記のような培養を行つている場合、バクテ
リア等の汚染が激しくD.O.が低下する時があ
る。そのような時には、培養液を適宜新鮮なも
のと入れ換えることにより、D.O.を維持する
ことができる。 When culturing as described above, there are times when there is severe contamination with bacteria and the like, resulting in a decrease in DO. In such cases, DO can be maintained by replacing the culture medium with fresh culture medium.
なお、以上の各手段を２個以上組み合わせても
良い。 Note that two or more of the above-mentioned means may be combined.
(e) 実施例
実施例 １
アルテミアの耐久卵を28℃の60％天然海水中で
24時間放置し、孵化させた。孵化後のアルテミア
幼生を集め、特願昭60−227977号記載の方法によ
り海産クロレラ（Chlorella minutissima）をス
プレードライ後、高圧ホモジナイズ処理により分
散した飼料を用いて100パンライト水槽中、培
養液１ml当り約30個体で養成を行つた。(e) Examples Example 1 Durable eggs of Artemia were placed in 60% natural seawater at 28°C.
The eggs were left for 24 hours to hatch. Artemia larvae after hatching were collected, spray-dried with marine chlorella (Chlorella minutissima) by the method described in Japanese Patent Application No. 60-227977, and then placed in a 100 Panlite aquarium using feed dispersed by high-pressure homogenization, per ml of culture solution. Approximately 30 individuals were trained.
その時の通気量を毎分、実験区10、３
、１で行なつたところ、第１図に示した結
果が得られた。実験区では、24時間後に全個体
が死滅した。また、実験区では、と比較した
場合、生育のスピードも遅く、体長が２mmサイズ
になるまでに生残率が30％まで低下した。実験区
では、生残率が100％で、６日間の養成により、
体長2.0〜2.5mmとなつた。 At that time, the airflow rate per minute was determined in experimental areas 10 and 3.
, 1, the results shown in FIG. 1 were obtained. In the experimental area, all individuals died after 24 hours. In addition, in the experimental plots, the growth rate was slower than in the experimental plots, and the survival rate decreased to 30% by the time the body length reached 2 mm. In the experimental area, the survival rate was 100%, and after 6 days of training,
The body length was 2.0 to 2.5 mm.
なお、上記の実験において、ウインクラー法で
培養液中のD.O.を測定したところ、天然海水中
のD.O.の最高値は7.0ppm、実験区では６日目
に4.8pm、では６日目に1.8ppm、では２日目
に1ppm以下であつた。 In addition, in the above experiment, when DO in the culture solution was measured using the Winkler method, the highest value of DO in natural seawater was 7.0 ppm, 4.8 pm on the 6th day in the experimental area, and 1.8 ppm on the 6th day in the experimental area. , it was less than 1 ppm on the second day.
実施例 ２
アルテミアの耐久卵を28℃の60％天然海水中で
24時間放置し、孵化させた。孵化後、アルテミア
幼生を集め、100mlパンライト水槽中で実施例１
と同じ飼料を用いて、培養液１ml当り約30固体、
通気量毎分５で養成を行つた。Example 2 Durable eggs of Artemia were placed in 60% natural seawater at 28°C.
The eggs were left for 24 hours to hatch. After hatching, the Artemia larvae were collected and placed in a 100ml panlite aquarium in Example 1.
Approximately 30 solids per ml of culture solution using the same feed as
Training was performed at an air flow rate of 5 per minute.
その際、通気と同時に次のような実験区で生育
および生残率の比較を行つた。 At that time, at the same time as aeration, growth and survival rates were compared in the following experimental plots.
実験区 毎分５の通気のみ（コントロール）
圧縮酸素（酸素濃度99.6％）毎分10ml
吹き込み
培養液の入れ換え、毎日50
その結果、第２図に示すようにのみ生残率の
低下、体長の伸びが悪く、その他は生残率100％
で６日間で体長が2.0〜2.5mmとなり良好であつ
た。Experimental area Air flow only at 5 per minute (control) Compressed oxygen (oxygen concentration 99.6%) 10 ml per minute
Inflation Replace the culture solution at 50% every day. As a result, only the survival rate decreased and body length growth was poor, as shown in Figure 2, and the survival rate was 100% in other cases.
The body length was 2.0 to 2.5 mm in 6 days, which was good.
なお、上記の実験においてウインクラー法によ
りD.O.を測定したところ実験、は６日目に
4.0ppm以上、は2.5ppmであつた。 In addition, in the above experiment, when DO was measured by the Winkler method, the experiment was performed on the 6th day.
4.0ppm or higher was 2.5ppm.
第１図および第２図は実施例１および実施例２
におけるアルテミアの生残率と体長の変化を夫々
示す。
Figures 1 and 2 are Example 1 and Example 2.
The survival rate and body length changes of Artemia are shown respectively.
Claims (1)
素の吹き込み、および培養液の入れ換えの１種ま
たは２種以上の手段を組み合わせて、培養液の溶
存酸素濃度を3ppm以上を保つことを特徴とする
アルテミアの高密度養成方法。1. When cultivating Artemia, the dissolved oxygen concentration of the culture solution is maintained at 3 ppm or more by using one or more of the following methods: aeration, blowing gaseous oxygen, and replacing the culture solution. High-density training method for Artemia.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61063701A JPS62220155A (en) | 1986-03-19 | 1986-03-19 | High-density rearing of artemia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61063701A JPS62220155A (en) | 1986-03-19 | 1986-03-19 | High-density rearing of artemia |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62220155A JPS62220155A (en) | 1987-09-28 |
| JPH0425768B2 true JPH0425768B2 (en) | 1992-05-01 |
Family
ID=13236940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP61063701A Granted JPS62220155A (en) | 1986-03-19 | 1986-03-19 | High-density rearing of artemia |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62220155A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994069A (en) * | 2016-06-22 | 2016-10-12 | 安庆市皖宜季牛水产养殖有限责任公司 | Disease resisting and yield increasing culture method for freshwater shrimps in spring |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1195088A1 (en) * | 2000-10-05 | 2002-04-10 | Inve Technologies N.V. | Method for producing free swimming Artemia nauplii and packaged cysts for use in that method |
| CN107484688A (en) * | 2017-09-20 | 2017-12-19 | 苏州市相城区阳澄湖镇剑成水产生态养殖专业合作社 | The set of Taihu Lake freshwater shrimp and white shrimp supports method |
| JP7353730B2 (en) * | 2018-02-15 | 2023-10-02 | 株式会社ニッスイ | How to train Artemia |
| CN109197716A (en) * | 2018-09-19 | 2019-01-15 | 沈阳创普华农业投资有限公司 | A kind of method that cray seed greenhouse is breeding graded |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55138340A (en) * | 1979-04-13 | 1980-10-29 | Asahi Chemical Ind | Method and apparatus for culturing plankton |
| JPS5978635A (en) * | 1982-09-24 | 1984-05-07 | シンフィナ・オレオフィナ・ソシエテ・アノニム | Method and apparatus for propagating artemia larvae |
| JPS62126922A (en) * | 1985-11-27 | 1987-06-09 | 日清製油株式会社 | Culture of biological feed |
-
1986
- 1986-03-19 JP JP61063701A patent/JPS62220155A/en active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55138340A (en) * | 1979-04-13 | 1980-10-29 | Asahi Chemical Ind | Method and apparatus for culturing plankton |
| JPS5978635A (en) * | 1982-09-24 | 1984-05-07 | シンフィナ・オレオフィナ・ソシエテ・アノニム | Method and apparatus for propagating artemia larvae |
| JPS62126922A (en) * | 1985-11-27 | 1987-06-09 | 日清製油株式会社 | Culture of biological feed |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994069A (en) * | 2016-06-22 | 2016-10-12 | 安庆市皖宜季牛水产养殖有限责任公司 | Disease resisting and yield increasing culture method for freshwater shrimps in spring |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62220155A (en) | 1987-09-28 |
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