JPH0420589A - Manufacture of green tea polyphenol - Google Patents
Manufacture of green tea polyphenolInfo
- Publication number
- JPH0420589A JPH0420589A JP2124166A JP12416690A JPH0420589A JP H0420589 A JPH0420589 A JP H0420589A JP 2124166 A JP2124166 A JP 2124166A JP 12416690 A JP12416690 A JP 12416690A JP H0420589 A JPH0420589 A JP H0420589A
- Authority
- JP
- Japan
- Prior art keywords
- tea
- organic solvent
- green tea
- extract
- alcohol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 16
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 16
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 7
- 244000269722 Thea sinensis Species 0.000 title abstract description 30
- 235000009569 green tea Nutrition 0.000 title abstract description 6
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims abstract description 13
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 239000003960 organic solvent Substances 0.000 claims description 13
- 238000000605 extraction Methods 0.000 claims description 8
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000005192 partition Methods 0.000 claims description 4
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 4
- DKPFZGUDAPQIHT-UHFFFAOYSA-N Butyl acetate Natural products CCCCOC(C)=O DKPFZGUDAPQIHT-UHFFFAOYSA-N 0.000 claims description 2
- NTIZESTWPVYFNL-UHFFFAOYSA-N Methyl isobutyl ketone Chemical compound CC(C)CC(C)=O NTIZESTWPVYFNL-UHFFFAOYSA-N 0.000 claims description 2
- UIHCLUNTQKBZGK-UHFFFAOYSA-N Methyl isobutyl ketone Natural products CCC(C)C(C)=O UIHCLUNTQKBZGK-UHFFFAOYSA-N 0.000 claims description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 claims description 2
- KXKVLQRXCPHEJC-UHFFFAOYSA-N acetic acid trimethyl ester Natural products COC(C)=O KXKVLQRXCPHEJC-UHFFFAOYSA-N 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 235000019441 ethanol Nutrition 0.000 claims description 2
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 claims description 2
- YKYONYBAUNKHLG-UHFFFAOYSA-N n-Propyl acetate Natural products CCCOC(C)=O YKYONYBAUNKHLG-UHFFFAOYSA-N 0.000 claims description 2
- 229940090181 propyl acetate Drugs 0.000 claims description 2
- NQPDZGIKBAWPEJ-UHFFFAOYSA-N valeric acid Chemical compound CCCCC(O)=O NQPDZGIKBAWPEJ-UHFFFAOYSA-N 0.000 claims description 2
- 241001122767 Theaceae Species 0.000 claims 5
- 239000002904 solvent Substances 0.000 abstract description 4
- 239000000203 mixture Substances 0.000 abstract 1
- 235000013616 tea Nutrition 0.000 description 23
- 238000007796 conventional method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 235000006468 Thea sinensis Nutrition 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 235000020279 black tea Nutrition 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000020333 oolong tea Nutrition 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Anti-Oxidant Or Stabilizer Compositions (AREA)
Abstract
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は茶ポリフェノールの製造方法に関する。[Detailed description of the invention] [Industrial application field] The present invention relates to a method for producing tea polyphenols.
〔従来の技術、発明が解決しようとする課題〕茶成分の
生理活性、特に茶ポリフェノールの生理活性については
抗酸化作用、抗腫瘍作用、抗菌作用が報告されている。[Prior Art and Problems to be Solved by the Invention] It has been reported that the physiological activities of tea components, particularly tea polyphenols, include antioxidant, antitumor, and antibacterial effects.
茶よりポリフェノールを製造する方法も報告されている
が、大量の溶剤を使用したり、また操作が煩雑である。A method for producing polyphenols from tea has also been reported, but this method requires the use of a large amount of solvent and is complicated to operate.
茶ポリフェノールを有効に利用するためには効率よく簡
便に製造する方法が望まれている。In order to effectively utilize tea polyphenols, an efficient and simple production method is desired.
そこで、本発明者らは検討を重ねた結果、茶ポリフェノ
ールを効率よく製造する方法を見出し、本発明を完成し
た。As a result of repeated studies, the present inventors discovered a method for efficiently producing tea polyphenols and completed the present invention.
すなわち本発明は、茶を水で抽出し、得られた抽出液を
濃縮した後、親水性有機溶媒を加え高分子物質などを不
溶化して除き、次いで有機溶媒を加え分配抽出し、有機
溶媒層を分取することを特徴とする茶ポリフェノールの
製造方法に関する。That is, in the present invention, tea is extracted with water, the resulting extract is concentrated, a hydrophilic organic solvent is added to insolubilize and remove polymeric substances, and then an organic solvent is added to carry out partition extraction to separate the organic solvent layer. The present invention relates to a method for producing tea polyphenols, which comprises separating the polyphenols.
本発明において原料である茶としては各種形態のものが
あり、例えば茶生葉１発酵茶、半発酵茶不発酵茶などが
挙げられる。また、茶を抽出する代わりにインスタント
緑茶、インスタント紅茶。In the present invention, the raw material tea is available in various forms, such as single-fermented tea, semi-fermented tea, and unfermented tea. Also, instant green tea, instant black tea instead of brewing tea.
インスタントウーロン茶などを水に溶解して用いても構
わない。Instant oolong tea or the like may be dissolved in water and used.
茶を水抽出する方法については制限ｈＸ”ないが、好ま
しくは熱水を用いて抽出を行う。抽出方法は攪拌抽出、
多段向流抽出など従来の方法により行えばよく、十分に
茶ポリフェノールが抽出されればよい。There are no restrictions on the method of extracting tea with water, but it is preferable to extract using hot water.Extraction methods include stirring extraction,
Conventional methods such as multistage countercurrent extraction may be used as long as tea polyphenols are sufficiently extracted.
次に、高分子物質などを不溶化させるために用いる親水
性有機溶媒としては、メチルアルコールエチルアルコー
ル、ｎ−プロピルアルコール、イソプロピルアルコール
およびアセトンから選ばれた少な（とも１種のものがあ
り、その添加量は、親水性有機溶媒の濃度が２０〜９０
％になるように加え、好ましくは４０〜７０％である。Next, as the hydrophilic organic solvent used to insolubilize polymeric substances, there are a few selected from methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol, and acetone. The amount is such that the concentration of the hydrophilic organic solvent is between 20 and 90.
%, preferably 40 to 70%.
不溶化した高分子物質などは常法により除去する。Insolubilized polymer substances are removed by conventional methods.
次いで、これに有＠溶媒を加えて分配抽出する。Next, a solvent is added to this for partition extraction.
ここで用いる有機溶媒としては酢酸メチル、酢酸エチル
、酢酸プロピル、酢酸ブチル、メチルイソブチルケトン
などがある。これらを単独でもしくは適宜組合せて用い
て分配抽出し、有機溶媒層に茶ポリフェノールを移行さ
せる。Examples of organic solvents used here include methyl acetate, ethyl acetate, propyl acetate, butyl acetate, and methyl isobutyl ketone. Distributive extraction is performed using these alone or in an appropriate combination, and tea polyphenols are transferred to the organic solvent layer.
このようにして得られた茶ポリフェノール有機溶媒溶液
を濃縮、さらに必要に応じて常法により乾燥粉末化して
茶ポリフェノール粉末が得られる。The thus obtained tea polyphenol organic solvent solution is concentrated and, if necessary, dried and powdered by a conventional method to obtain a tea polyphenol powder.
次に、本発明を実施例により詳しく説明する。 Next, the present invention will be explained in detail with reference to examples.
実施例
煎茶１ｋｇを９０°Ｃの熱水１０！で１時間攪拌しなが
ら抽出し、茶葉をろ過により除き、８．３ｆの抽出液を
得た。この液を１尼まで濃縮し、これにアセトン１！を
加えて攪拌し、生じた不溶物を遠心分離により除いた。Example: 1 kg of Sencha green tea with 90°C hot water 10 times! The tea leaves were extracted with stirring for 1 hour, and the tea leaves were removed by filtration to obtain an 8.3f extract. Concentrate this solution to 1 part, add 1 part acetone! was added and stirred, and the resulting insoluble matter was removed by centrifugation.
上清液に酢酸エチル１！を加えて攪拌し、３０分間静置
した。得られた酢酸エチル層を減圧上濃縮し、水相に転
挟した後、凍結乾燥して純度６０％の茶ポリフェノール
を９７ｇ得た。1 ethyl acetate in the supernatant! was added, stirred, and allowed to stand for 30 minutes. The obtained ethyl acetate layer was concentrated under reduced pressure, transferred to an aqueous phase, and then freeze-dried to obtain 97 g of tea polyphenols with a purity of 60%.
本発明によれば、茶から効率的に高純度の茶ポリフェノ
ールを容易に製造することができる。According to the present invention, highly pure tea polyphenols can be easily produced efficiently from tea.
Claims (4)
親水性有機溶媒を加えたのち有機溶媒にて分配抽出し、
この有機溶媒層を分取することを特徴とする茶ポリフェ
ノールの製造方法。(1) Extract tea with water, concentrate the resulting extract, add a hydrophilic organic solvent to it, and then perform partition extraction with an organic solvent,
A method for producing tea polyphenols, which comprises separating this organic solvent layer.
載の方法。(2) The method according to claim 1, wherein the tea is unfermented tea or semi-fermented tea.
コール、ｎ−プロピルアルコール、イソプスピルアルコ
ールおよびアセトンの中から選ばれた少なくとも１種の
ものである請求項１に記載の方法。(3) The method according to claim 1, wherein the hydrophilic organic solvent is at least one selected from methyl alcohol, ethyl alcohol, n-propyl alcohol, isopropyl alcohol, and acetone.
酢酸エチル、酢酸プロピル、酢酸ブチルおよびメチルイ
ソブチルケトンから選ばれた少なくとも１種のものであ
る請求項１に記載の方法。(4) The organic solvent added for partition extraction is methyl acetate,
The method according to claim 1, which is at least one selected from ethyl acetate, propyl acetate, butyl acetate and methyl isobutyl ketone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2124166A JPH0420589A (en) | 1990-05-16 | 1990-05-16 | Manufacture of green tea polyphenol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2124166A JPH0420589A (en) | 1990-05-16 | 1990-05-16 | Manufacture of green tea polyphenol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0420589A true JPH0420589A (en) | 1992-01-24 |
Family
ID=14878583
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2124166A Pending JPH0420589A (en) | 1990-05-16 | 1990-05-16 | Manufacture of green tea polyphenol |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0420589A (en) |
Cited By (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001039881A (en) * | 1999-07-26 | 2001-02-13 | Asahi Breweries Ltd | Cancer cell metastasis inhibitor with tea extract as active ingredient |
| KR100423241B1 (en) * | 2001-09-21 | 2004-03-18 | 한미향료화학주식회사 | Method of refining and microcapsuling tea extract |
| JP2004180535A (en) * | 2002-11-29 | 2004-07-02 | Kao Corp | Method for decaffeinating caffein-containing catechin composition |
| US7056548B2 (en) | 2003-10-31 | 2006-06-06 | Kao Corporation | Packaged, tea-based beverages |
| WO2007099715A1 (en) | 2006-03-02 | 2007-09-07 | Kao Corporation | Process for producing purified tea extract |
| WO2007122818A1 (en) | 2006-04-17 | 2007-11-01 | Kao Corporation | Process for producing purified green tea extract |
| WO2007122817A1 (en) | 2006-04-17 | 2007-11-01 | Kao Corporation | Process for producing purified green tea extract |
| WO2007129472A1 (en) | 2006-05-09 | 2007-11-15 | Kao Corporation | Tea beverage packed in container |
| WO2007132562A1 (en) | 2006-05-16 | 2007-11-22 | Kao Corporation | Beverage packed in container |
| WO2008004340A1 (en) | 2006-07-05 | 2008-01-10 | Kao Corporation | Senescence inhibitor |
| WO2008004333A1 (en) | 2006-07-05 | 2008-01-10 | Kao Corporation | Agent for improving muscle power |
| WO2008029511A1 (en) | 2006-09-08 | 2008-03-13 | Kao Corporation | Insulin sensitivity improving agent |
| WO2008146479A1 (en) | 2007-05-24 | 2008-12-04 | Kao Corporation | Purified green tea extract |
| WO2009019876A1 (en) | 2007-08-08 | 2009-02-12 | Kao Corporation | Method for production of tea extract |
| WO2009028211A1 (en) | 2007-08-30 | 2009-03-05 | Kao Corporation | Instant powder drink |
| WO2009084215A1 (en) | 2007-12-27 | 2009-07-09 | Kao Corporation | Process for producing purified tea extract |
| EP2253223A1 (en) | 2003-12-02 | 2010-11-24 | Kao Corporation | Package drink |
| US7883734B2 (en) | 2002-10-28 | 2011-02-08 | Kao Corporation | Method of removing caffeine from caffeine-containing catechin compound composition |
| WO2011024471A1 (en) | 2009-08-27 | 2011-03-03 | 小川香料株式会社 | Taste-improving agent for sweetener having high sweetness |
| WO2011049125A1 (en) | 2009-10-21 | 2011-04-28 | 花王株式会社 | Ice cream |
| WO2011071056A1 (en) | 2009-12-09 | 2011-06-16 | 花王株式会社 | Method for producing purified tea extract |
| WO2011078119A1 (en) | 2009-12-21 | 2011-06-30 | 花王株式会社 | Instant black tea |
| WO2012091135A1 (en) | 2010-12-28 | 2012-07-05 | 花王株式会社 | Method for producing purified tea extract |
| WO2013094494A1 (en) | 2011-12-21 | 2013-06-27 | 花王株式会社 | Method for producing purified tea extract |
| US8841236B2 (en) | 2010-07-16 | 2014-09-23 | Kao Corporation | Method for imparting stress tolerance to plant, plant stress tolerance imparting composition and use thereof |
| CN104757190A (en) * | 2015-04-14 | 2015-07-08 | 福建师范大学 | Method for extracting tea polyphenol by extraction-adsorption method |
| CN105533730A (en) * | 2015-12-18 | 2016-05-04 | 安徽红星药业股份有限公司 | Preparation method of fat-soluble tea polyphenol |
| WO2018225482A1 (en) | 2017-06-07 | 2018-12-13 | 花王株式会社 | Ammonia metabolism promoter |
| WO2018225618A1 (en) | 2017-06-07 | 2018-12-13 | 花王株式会社 | Ammonia metabolism promoter |
-
1990
- 1990-05-16 JP JP2124166A patent/JPH0420589A/en active Pending
Cited By (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001039881A (en) * | 1999-07-26 | 2001-02-13 | Asahi Breweries Ltd | Cancer cell metastasis inhibitor with tea extract as active ingredient |
| KR100423241B1 (en) * | 2001-09-21 | 2004-03-18 | 한미향료화학주식회사 | Method of refining and microcapsuling tea extract |
| US9198946B2 (en) | 2002-10-28 | 2015-12-01 | Kao Corporation | Green tea extract |
| US7883734B2 (en) | 2002-10-28 | 2011-02-08 | Kao Corporation | Method of removing caffeine from caffeine-containing catechin compound composition |
| EP2382876A1 (en) | 2002-10-28 | 2011-11-02 | Kao Corporation | Method of removing caffeine from caffeine-containing catechin compound composition |
| EP2382877A1 (en) | 2002-10-28 | 2011-11-02 | Kao Corporation | Method of removing caffeine from caffeine-containing catechin compound composition |
| US8574655B2 (en) | 2002-10-28 | 2013-11-05 | Kao Corporation | Packaged beverage with caffeine-containing catechin composition |
| EP2659791A1 (en) | 2002-10-28 | 2013-11-06 | Kao Corporation | Method of removing caffeine from caffeine-containing catechin compound composition |
| JP2004180535A (en) * | 2002-11-29 | 2004-07-02 | Kao Corp | Method for decaffeinating caffein-containing catechin composition |
| US7056548B2 (en) | 2003-10-31 | 2006-06-06 | Kao Corporation | Packaged, tea-based beverages |
| EP2253223A1 (en) | 2003-12-02 | 2010-11-24 | Kao Corporation | Package drink |
| WO2007099715A1 (en) | 2006-03-02 | 2007-09-07 | Kao Corporation | Process for producing purified tea extract |
| WO2007122817A1 (en) | 2006-04-17 | 2007-11-01 | Kao Corporation | Process for producing purified green tea extract |
| WO2007122818A1 (en) | 2006-04-17 | 2007-11-01 | Kao Corporation | Process for producing purified green tea extract |
| WO2007129472A1 (en) | 2006-05-09 | 2007-11-15 | Kao Corporation | Tea beverage packed in container |
| WO2007132562A1 (en) | 2006-05-16 | 2007-11-22 | Kao Corporation | Beverage packed in container |
| WO2008004333A1 (en) | 2006-07-05 | 2008-01-10 | Kao Corporation | Agent for improving muscle power |
| WO2008004340A1 (en) | 2006-07-05 | 2008-01-10 | Kao Corporation | Senescence inhibitor |
| WO2008029511A1 (en) | 2006-09-08 | 2008-03-13 | Kao Corporation | Insulin sensitivity improving agent |
| WO2008146479A1 (en) | 2007-05-24 | 2008-12-04 | Kao Corporation | Purified green tea extract |
| WO2009019876A1 (en) | 2007-08-08 | 2009-02-12 | Kao Corporation | Method for production of tea extract |
| WO2009028211A1 (en) | 2007-08-30 | 2009-03-05 | Kao Corporation | Instant powder drink |
| WO2009084215A1 (en) | 2007-12-27 | 2009-07-09 | Kao Corporation | Process for producing purified tea extract |
| WO2011024471A1 (en) | 2009-08-27 | 2011-03-03 | 小川香料株式会社 | Taste-improving agent for sweetener having high sweetness |
| WO2011049125A1 (en) | 2009-10-21 | 2011-04-28 | 花王株式会社 | Ice cream |
| WO2011071056A1 (en) | 2009-12-09 | 2011-06-16 | 花王株式会社 | Method for producing purified tea extract |
| WO2011078119A1 (en) | 2009-12-21 | 2011-06-30 | 花王株式会社 | Instant black tea |
| US8841236B2 (en) | 2010-07-16 | 2014-09-23 | Kao Corporation | Method for imparting stress tolerance to plant, plant stress tolerance imparting composition and use thereof |
| WO2012091135A1 (en) | 2010-12-28 | 2012-07-05 | 花王株式会社 | Method for producing purified tea extract |
| WO2013094494A1 (en) | 2011-12-21 | 2013-06-27 | 花王株式会社 | Method for producing purified tea extract |
| CN104757190A (en) * | 2015-04-14 | 2015-07-08 | 福建师范大学 | Method for extracting tea polyphenol by extraction-adsorption method |
| CN105533730A (en) * | 2015-12-18 | 2016-05-04 | 安徽红星药业股份有限公司 | Preparation method of fat-soluble tea polyphenol |
| WO2018225482A1 (en) | 2017-06-07 | 2018-12-13 | 花王株式会社 | Ammonia metabolism promoter |
| WO2018225618A1 (en) | 2017-06-07 | 2018-12-13 | 花王株式会社 | Ammonia metabolism promoter |
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