JPH04141084A - Carrier for serum-free cell culture - Google Patents
Carrier for serum-free cell cultureInfo
- Publication number
- JPH04141084A JPH04141084A JP2260522A JP26052290A JPH04141084A JP H04141084 A JPH04141084 A JP H04141084A JP 2260522 A JP2260522 A JP 2260522A JP 26052290 A JP26052290 A JP 26052290A JP H04141084 A JPH04141084 A JP H04141084A
- Authority
- JP
- Japan
- Prior art keywords
- serum
- carrier
- cells
- calcium phosphate
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004113 cell culture Methods 0.000 title claims description 15
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 13
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 12
- 235000011010 calcium phosphates Nutrition 0.000 claims abstract description 12
- 230000002062 proliferating effect Effects 0.000 claims abstract description 11
- -1 calcium phosphate compound Chemical class 0.000 claims abstract description 10
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 239000012876 carrier material Substances 0.000 claims abstract description 4
- 239000012679 serum free medium Substances 0.000 claims description 12
- 230000001464 adherent effect Effects 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 6
- 210000004027 cell Anatomy 0.000 abstract description 26
- 229910052588 hydroxylapatite Inorganic materials 0.000 abstract description 5
- 239000000203 mixture Substances 0.000 abstract description 5
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 abstract description 5
- 239000001963 growth medium Substances 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 3
- 210000002889 endothelial cell Anatomy 0.000 abstract description 2
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 239000004017 serum-free culture medium Substances 0.000 abstract 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 1
- 239000000853 adhesive Substances 0.000 abstract 1
- 230000001070 adhesive effect Effects 0.000 abstract 1
- 229910052791 calcium Inorganic materials 0.000 abstract 1
- 239000011575 calcium Substances 0.000 abstract 1
- 210000002950 fibroblast Anatomy 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 14
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 11
- 239000008187 granular material Substances 0.000 description 6
- 239000004310 lactic acid Substances 0.000 description 6
- 235000014655 lactic acid Nutrition 0.000 description 6
- 210000002966 serum Anatomy 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000002245 particle Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 3
- MCMNRKCIXSYSNV-UHFFFAOYSA-N Zirconium dioxide Chemical compound O=[Zr]=O MCMNRKCIXSYSNV-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000001354 calcination Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 235000020774 essential nutrients Nutrition 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 229910052586 apatite Inorganic materials 0.000 description 1
- 235000021152 breakfast Nutrition 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 108010015046 cell aggregation factors Proteins 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 229910052587 fluorapatite Inorganic materials 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- VSIIXMUUUJUKCM-UHFFFAOYSA-D pentacalcium;fluoride;triphosphate Chemical compound [F-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O VSIIXMUUUJUKCM-UHFFFAOYSA-D 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000003760 tallow Substances 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 description 1
- 229910000391 tricalcium phosphate Inorganic materials 0.000 description 1
- 235000019731 tricalcium phosphate Nutrition 0.000 description 1
- 229940078499 tricalcium phosphate Drugs 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/10—Mineral substrates
- C12N2533/18—Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells
Abstract
Description
【発明の詳細な説明】
〈産業上の利用分野〉
本発明は、接着増殖性細胞を無血清培地で培養するため
の無血清細胞培養担体及び該担体を充填してなるカラム
式連続培養装置に関する。Detailed Description of the Invention <Industrial Application Field> The present invention relates to a serum-free cell culture carrier for culturing adherent proliferative cells in a serum-free medium, and a column-type continuous culture device filled with the carrier. .
〈従来の技術〉
従来、細胞培養方法においては、アミノ酸、ビタミン、
必須栄養物、無機塩類等を加えた合成培地に動物血清を
１０％程度加えた培地を使用すること（組織培養の技術
、朝食書店、１９８８）が知られている。しかしながら
、前記動物血清は製造ロフトによる品質の差が大きく、
細胞を培養する際に最適なロフトを選択する必要がある
。また前記動物血清の供給は必ずしも安定しておらず、
工業的規模に適さないという問題がある。更に前記動物
血清を添加した培地においては、血清タンパク質が高濃
度に存在するため、細胞の産生ずる有用物質の分離が困
難であるという問題もある。<Conventional technology> Conventionally, in cell culture methods, amino acids, vitamins,
It is known to use a synthetic medium containing essential nutrients, inorganic salts, etc., to which approximately 10% animal serum is added (Tissue Culture Techniques, Breakfast Shoten, 1988). However, the quality of the animal serum varies greatly depending on the manufacturing loft.
It is necessary to select the optimal loft when culturing cells. Furthermore, the supply of the animal serum is not necessarily stable;
The problem is that it is not suitable for industrial scale. Furthermore, in the culture medium to which animal serum is added, serum proteins are present at a high concentration, making it difficult to separate useful substances produced by the cells.
〈発明が解決しようとする課題〉
本発明の目的は、無血清培地で接着増殖性細胞を培養す
るための無血清細胞培養担体を提供することにある。<Problems to be Solved by the Invention> An object of the present invention is to provide a serum-free cell culture carrier for culturing adherent proliferative cells in a serum-free medium.
本発明の別の目的は、無血清培地を通過させることによ
って、連続的に細胞を増殖、分化させることができ、且
つ細胞が産生ずる有用物質を効率よく収集することがで
きるカラム式連続培養装置を提供することにある。Another object of the present invention is to provide a column-type continuous culture device that is capable of continuously proliferating and differentiating cells by passing a serum-free medium therethrough, and efficiently collecting useful substances produced by the cells. Our goal is to provide the following.
〈課題を解決するための手段〉
本発明によれば、リン酸カルシウム化合物を含む担体材
料からなる接着増殖性細胞を無血清培地で培養するため
の無血清細胞培養担体が提供される。<Means for Solving the Problems> According to the present invention, there is provided a serum-free cell culture carrier for culturing adherent proliferative cells in a serum-free medium, which is made of a carrier material containing a calcium phosphate compound.
また本発明によれば無血清培地を通過させることによっ
て、連続的に細胞を増殖、分化させ、且つ得られる溶出
液により、前記細胞が産生ずる有用物質を効率よく収集
するための培養装置であって、該培養装置が、前記無血
清細胞培養担体を管内に充填してなることを特徴とする
カラム式連続培養装置が提供される。Further, according to the present invention, there is provided a culture device for continuously proliferating and differentiating cells by passing through a serum-free medium, and efficiently collecting useful substances produced by the cells using the obtained eluate. Accordingly, there is provided a column-type continuous culture device, characterized in that the culture device is formed by filling a tube with the serum-free cell culture carrier.
以下本発明を更に詳細に説明する。The present invention will be explained in more detail below.
本発明の無血清細胞培養担体は、接着増殖性細胞、具体
的には例えば株化された前原性細胞ＭＣ３Ｔ３−Ｅｌ、
各種線維非細胞、内皮細胞、筋細胞、神経細胞またはこ
れらの腫瘍細胞、更にこれらに外来性の遺伝子を導入し
た細胞等を無血清培地で培養するための担体であって、
リン酸カルシウム化合物を含む担体材料を成分とするこ
とを特徴とする。The serum-free cell culture carrier of the present invention comprises adherent proliferative cells, specifically, established progenic cell lines MC3T3-El,
A carrier for culturing various fibrous non-cells, endothelial cells, muscle cells, nerve cells or these tumor cells, as well as cells into which foreign genes have been introduced, in a serum-free medium,
It is characterized by containing a carrier material containing a calcium phosphate compound as a component.
本発明に用いるリン酸カルシウム化合物としては、例え
ば水酸アパタイト、リン酸三カルシウム、リン酸西カル
シウム、フッ素アパタイト、炭酸アパタイト及びこれら
の混合物等から成る群より選択される化合物又は混合物
を好ましく挙げることができ、特に細胞の付着性が良好
であり、且つ接着増殖性細胞の活性を高くすることがで
きろ水酸アパタイトを用いるのが望ましい。前記リン酸
カルシウム化合物の形状は、リアクター容器の種類によ
り５種々選択することができ、例えば多孔体。Preferable examples of the calcium phosphate compound used in the present invention include compounds or mixtures selected from the group consisting of hydroxyapatite, tricalcium phosphate, western calcium phosphate, fluoroapatite, carbonate apatite, and mixtures thereof. In particular, it is desirable to use hydroxyapatite because it has good cell adhesion and can increase the activity of adherent proliferative cells. The shape of the calcium phosphate compound can be selected from five types depending on the type of reactor container, such as a porous body.
顆粒状等とすることができる。また、リン酸カルシウム
化合物の比表面積（ガス吸着法による）は。It can be in the form of granules, etc. Also, the specific surface area of the calcium phosphate compound (by gas adsorption method) is.
０．１〜７０ｍ′／ｇの範囲であるのが好ましい。It is preferably in the range of 0.1 to 70 m'/g.
本発明の無血清細胞培養担体に用いる前記リン酸カルシ
ウム化合物を調製するには、公知の焼成方法等により得
ることができ、例えばリン酸カルシウムの焼成温度によ
りコントロールすることができる。The calcium phosphate compound used in the serum-free cell culture carrier of the present invention can be prepared by a known calcination method, and can be controlled by, for example, the calcination temperature of calcium phosphate.
また本発明では、前記無血清細胞培養担体を管内に充填
することにより、無血清の培地を通過させることによっ
て、連続的に細胞を増殖、分化させ、且つ得られる溶出
液から、前記細胞が産生ずる有用物質を収集するための
カラム式連続培養装置を得ることができる。前記無血清
細胞培養担体を管内に充填するには、例えばカラム等に
リン酸カルシウム化合物を充填し、接着増殖性細胞の培
地、即ち無血清の培地を添加する方法等により行うこと
ができる。前記無血清の培地を調製するには、既にイー
グルらによって確立されている動物細胞培養用の培地、
即ち最小必要培地の処方等に従って、無機塩類、アミノ
酸、ビタミン、必須栄養物等の溶液を調製し、滅菌した
後、必要に応じて細胞成長因子、ホルモン、細胞接着因
子等を添加する方法等により得ることができる。Further, in the present invention, cells are continuously proliferated and differentiated by filling a tube with the serum-free cell culture carrier and passing a serum-free medium therethrough, and the cells are produced from the resulting eluate. A column-type continuous culture device can be obtained for collecting the resulting useful substances. The serum-free cell culture carrier can be filled into a tube by, for example, filling a column with a calcium phosphate compound and adding a medium for adherent proliferating cells, that is, a serum-free medium. To prepare the serum-free medium, a medium for animal cell culture, which has already been established by Eagle et al.
In other words, a solution of inorganic salts, amino acids, vitamins, essential nutrients, etc. is prepared according to the minimum required medium prescription, etc., and after sterilization, cell growth factors, hormones, cell adhesion factors, etc. are added as necessary. Obtainable.
〈発明の効果〉
本発明の無血清細胞培養担体は、使用する培地中に血清
を含まないので、組成の一定な培地を安定して供給する
ことができ、また細胞に適したロットを選択する必要が
なく培養操作が簡便である。<Effects of the Invention> Since the serum-free cell culture carrier of the present invention does not contain serum in the medium used, a medium with a constant composition can be stably supplied, and a lot suitable for the cells can be selected. It is not necessary and the culture operation is simple.
また前記無血清細胞培養担体を充填したカラム式連続培
養装置は、連続的に細胞を培地、分化させることができ
、且つ付着した細胞が作り出す有用物質を、余分なタン
パク質が存在しないので効率よく分離・精製することが
できるのでバイオリアクターとして極めて有用である。In addition, the column-type continuous culture device filled with the serum-free cell culture carrier allows cells to be continuously cultured and differentiated, and useful substances produced by attached cells can be efficiently separated because there are no excess proteins.・It is extremely useful as a bioreactor because it can be purified.
〈実施例〉
以下本発明を実施例及び比較例により詳細に説明するが
、本発明は、これらに限定されるものではない。<Examples> The present invention will be described in detail below using Examples and Comparative Examples, but the present invention is not limited thereto.
ヌ」１」１
比表面積１０ボ／ｇ、粒径０．３〜０．５ｍｍの水酸ア
パタイト顆粒を体積１０ｍＱ、カラムサイズ２、Ｏａｎ
φＸ３．２ａｎＬのカラムに充填し、次いで予め骨原住
細胞株ＭＣ３Ｔ３−Ｅｌ細胞をＩ Ｘ １０’個カラム
に播種した。播種後３日間は１０％牛脂児血清を添加し
たα−ＭＥＭ培地で培養し、以降は無血清α−ＭＥＭ培
地で培養した９培地は２日おきに交換し、その際交換し
た培養後の培地中の乳酸濃度を、乳酸脱水素酵素による
ＮＡＤの還元により測定した。結果を第１図に示す。Nu'1'1 Hydroxyapatite granules with a specific surface area of 10 bo/g and a particle size of 0.3 to 0.5 mm were collected in a volume of 10 mQ, column size of 2, Oan
A column of φX3.2 anL was filled, and then I×10' bone-dwelling cell line MC3T3-El cells were seeded in advance on the column. For 3 days after seeding, the culture was carried out in α-MEM medium supplemented with 10% tallow serum, and thereafter the 9 medium was cultured in serum-free α-MEM medium, which was replaced every two days. The lactic acid concentration therein was measured by reduction of NAD by lactate dehydrogenase. The results are shown in Figure 1.
比較例１〜３
カラムの充填材を１粒径０．３〜０．５ｍｍのアルミナ
顆粒（比較例１）、ジルコニア顆粒（比較例２）及び酸
化チタン顆粒（比較例３）にした以外は、実施例１と同
様にして、培地中の乳酸濃度を測定した。比較例１の結
果を第２図に、比較例２の結果を第３図に、また比較例
３の結果を第４図にそれぞれ示す。Comparative Examples 1 to 3 Except for using alumina granules (Comparative Example 1), zirconia granules (Comparative Example 2), and titanium oxide granules (Comparative Example 3) with particle diameters of 0.3 to 0.5 mm as the column fillers, In the same manner as in Example 1, the lactic acid concentration in the medium was measured. The results of Comparative Example 1 are shown in FIG. 2, the results of Comparative Example 2 are shown in FIG. 3, and the results of Comparative Example 3 are shown in FIG. 4.
笑凰何ス
粒径０．３〜０．５ｆ１１１の水酸アパタイト顆粒をカ
ラム体積５０ｍＱ、カラムサイズ３．０（！Ｉｔφ×７
．５ａＩＩＬのカラムに充填し、カラム式連続培養装置
を作製した。前記カラムにＭＣ３Ｔ３−Ｅｌ細胞を８．
２Ｘ１０Ｇ個播種し、連続的に無血清培地を、７０ｍＱ
／日の溶比速度で流し、２．４Ｑの培養培地を得た。次
いで得られた培養培地を５０ｍＱまで濃縮し、得られた
濃縮液を５ＤＳ−ポリアクリルアミドゲル電気泳動にか
けたところ、細胞の産生じたタンパク質が検出された。Hydroxyapatite granules with a particle size of 0.3 to 0.5f111 were added to a column with a volume of 50 mQ and a column size of 3.0 (!Itφ×7
．． A column of 5aIIL was filled to produce a column-type continuous culture device. 8. Add MC3T3-El cells to the column.
Seed 2x10G and continuously add serum-free medium to 70mQ
The cells were allowed to flow at a specific dissolution rate of 2.4Q/day to obtain a culture medium of 2.4Q. The resulting culture medium was then concentrated to 50 mQ, and the resulting concentrate was subjected to 5DS-polyacrylamide gel electrophoresis, and proteins produced by the cells were detected.
電気泳動の結果を第５図に示す。The results of electrophoresis are shown in FIG.
第１図は実施例１で培養した培地中の乳酸濃度と培養日
数との関係を示すグラフ、第２図は比較例１で培養した
培地中の乳酸濃度と培養日数との関係を示すグラフ、第
３図は比較例２で培養した培地中の乳酸濃度と培養日数
との関係を示すグラフ、第４図は比較例３で培養した培
地中の乳酸濃度と培養日数との関係を示すグラフ、第５
図は実施例２で得られた濃縮液を電気泳動にかけた際の
結果を示すチャートである。
ａ・・分子量マーカー、ｂ・・細胞生産タンパク質。FIG. 1 is a graph showing the relationship between the lactic acid concentration in the medium cultured in Example 1 and the number of culture days, and FIG. 2 is a graph showing the relationship between the lactic acid concentration in the medium cultured in Comparative Example 1 and the number of culture days. FIG. 3 is a graph showing the relationship between the lactic acid concentration in the medium cultured in Comparative Example 2 and the number of culture days, and FIG. 4 is a graph showing the relationship between the lactic acid concentration in the medium cultured in Comparative Example 3 and the number of culture days. Fifth
The figure is a chart showing the results when the concentrated solution obtained in Example 2 was subjected to electrophoresis. a: Molecular weight marker, b: Cell-produced protein.
Claims (1)
着増殖性細胞を無血清培地で培養するための無血清細胞
培養担体。 ２）無血清培地を通過させることによって、連続的に細
胞を増殖、分化させ、且つ得られる溶出液により、前記
細胞が産生する有用物質を効率よく収集するための培養
装置であって、該培養装置が、請求項１記載の無血清細
胞培養担体を管内に充填してなることを特徴とするカラ
ム式連続培養装置。[Scope of Claims] 1) A serum-free cell culture carrier for culturing adherent proliferative cells in a serum-free medium, which is made of a carrier material containing a calcium phosphate compound. 2) A culture device for continuously proliferating and differentiating cells by passing them through a serum-free medium, and efficiently collecting useful substances produced by the cells using the resulting eluate, the culture device A column-type continuous culture device, characterized in that the device has a tube filled with the serum-free cell culture carrier according to claim 1.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2260522A JPH04141084A (en) | 1990-10-01 | 1990-10-01 | Carrier for serum-free cell culture |
| GB9119709A GB2248628B (en) | 1990-10-01 | 1991-09-16 | Cell culturing in serum-free media |
| DE19914131375 DE4131375C2 (en) | 1990-10-01 | 1991-09-20 | Use of a calcium phosphate compound as a carrier for culturing cells in a serum-free medium |
| FR9112069A FR2667324B1 (en) | 1990-10-01 | 1991-10-01 | SUPPORT FOR CULTURE OF CELLS IN A SERUM FREE MEDIUM AND COLUMN TYPE DEVICE WITH SUCH SUPPORT. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2260522A JPH04141084A (en) | 1990-10-01 | 1990-10-01 | Carrier for serum-free cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH04141084A true JPH04141084A (en) | 1992-05-14 |
Family
ID=17349140
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2260522A Pending JPH04141084A (en) | 1990-10-01 | 1990-10-01 | Carrier for serum-free cell culture |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JPH04141084A (en) |
| DE (1) | DE4131375C2 (en) |
| FR (1) | FR2667324B1 (en) |
| GB (1) | GB2248628B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015211665A (en) * | 2014-01-29 | 2015-11-26 | ダイキン工業株式会社 | Temperature responsive substrate, manufacturing method of the same, and evaluation method of the same |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04278082A (en) * | 1991-03-02 | 1992-10-02 | Mitsubishi Materials Corp | Carrier for serum-free cell culture |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4757017A (en) * | 1984-09-14 | 1988-07-12 | Mcw Research Foundation, Inc. | In vitro cell culture system |
| JPS61282072A (en) * | 1985-06-07 | 1986-12-12 | Asahi Optical Co Ltd | Substrate for cell culture medium, device for cell culture and method therefore |
| DE3787805T2 (en) * | 1986-08-04 | 1994-02-10 | Univ New South Wales Kensingto | SERUM-FREE TISSUE CULTURE MEDIUM CONTAINING A POLYMER CELL PROTECTIVE. |
| JPS63198970A (en) * | 1987-02-16 | 1988-08-17 | Kirin Brewery Co Ltd | Hollow sphere of calcium phosphate |
| JPH02180707A (en) * | 1988-12-31 | 1990-07-13 | Tonen Corp | Production of phosphorus compound grain assemblage |
| JPH0367584A (en) * | 1989-08-04 | 1991-03-22 | Mitsubishi Materials Corp | Carrier for cell culture |
| JPH1042311A (en) * | 1996-07-23 | 1998-02-13 | Matsushita Electric Ind Co Ltd | Image signal processor |
-
1990
- 1990-10-01 JP JP2260522A patent/JPH04141084A/en active Pending
-
1991
- 1991-09-16 GB GB9119709A patent/GB2248628B/en not_active Expired - Fee Related
- 1991-09-20 DE DE19914131375 patent/DE4131375C2/en not_active Expired - Fee Related
- 1991-10-01 FR FR9112069A patent/FR2667324B1/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015211665A (en) * | 2014-01-29 | 2015-11-26 | ダイキン工業株式会社 | Temperature responsive substrate, manufacturing method of the same, and evaluation method of the same |
| US10689615B2 (en) | 2014-01-29 | 2020-06-23 | Daikin Industries, Ltd. | Temperature-responsive base material, method for producing same, and method for evaluating same |
Also Published As
| Publication number | Publication date |
|---|---|
| DE4131375C2 (en) | 1994-01-20 |
| GB2248628B (en) | 1994-09-07 |
| GB9119709D0 (en) | 1991-10-30 |
| FR2667324A1 (en) | 1992-04-03 |
| DE4131375A1 (en) | 1992-04-02 |
| FR2667324B1 (en) | 1995-06-16 |
| GB2248628A (en) | 1992-04-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4757017A (en) | In vitro cell culture system | |
| Schimmer | [52] Adrenocortical Y1 cells | |
| US20080009064A1 (en) | Temperature-Responsive Microcarrier | |
| Kumar et al. | The combined influence of substrate elasticity and surface-grafted molecules on the ex vivo expansion of hematopoietic stem and progenitor cells | |
| JPS60188062A (en) | Production of protein improved by using isotropic culture medium | |
| JP4233646B2 (en) | Method for increasing the stability and / or shelf life of various substrates | |
| CN107418930A (en) | A kind of preparation method purified with amplification human marrow mesenchymal stem cell | |
| CN109370985A (en) | A kind of human umbilical cord mesenchymal stem cells large-scale culture serum free medium | |
| ATE364408T1 (en) | METHOD FOR CULTIVATION OF CARTILAGE REPLACEMENT AND BIOMATRIX PRODUCED BY THIS METHOD | |
| Minamoto et al. | Development of a serum-free and heat-sterilizable medium and continuous high-density cell culture | |
| JPH04141084A (en) | Carrier for serum-free cell culture | |
| US5871999A (en) | Cell culture process and medium for the growth of adherent animal cells | |
| JPS5813396A (en) | Production of rat interloikin 2 from interloikin 2 non-producing malignant bacteria strain in presence of interloikin 1 | |
| Niwa et al. | Continuous culture of Reuber hepatoma cells in serum-free, arginine-, glutamine-and tyrosine-deprived chemically defined medium | |
| CA2111984A1 (en) | Iron chelate culture medium additive | |
| Schrimpf et al. | Growth of human vascular endothelial cells on various types of microcarriers | |
| US20040096943A1 (en) | Method and device for cultivating of cells at high densities and for obtaining of products from these cells | |
| CN112852742A (en) | Serum-free medium for inducing pluripotent stem cells and preparation method thereof | |
| JPH0367584A (en) | Carrier for cell culture | |
| JPS63209581A (en) | Embedding culture of attachment-dependent animal normal cell | |
| JPH04278082A (en) | Carrier for serum-free cell culture | |
| RU2201958C2 (en) | Dry sterile low-serum nutrient medium for mammalian cells culturing | |
| Watanabe et al. | THE RELATIONSHIP BETWEEN CELL‐SUBSTRATE ADHESIVENESS AND CELL GROWTH: A STUDY ON CHONDROCYTES CULTURED IN VITRO WITH CONDITIONED MEDIUM | |
| JPS63230080A (en) | Culture of animal cell | |
| Kim et al. | Mammalian cell cultivation on serum-coated microcarriers |