JPH0412272A - High-sensitivity dry process analysis piece - Google Patents
High-sensitivity dry process analysis pieceInfo
- Publication number
- JPH0412272A JPH0412272A JP11285790A JP11285790A JPH0412272A JP H0412272 A JPH0412272 A JP H0412272A JP 11285790 A JP11285790 A JP 11285790A JP 11285790 A JP11285790 A JP 11285790A JP H0412272 A JPH0412272 A JP H0412272A
- Authority
- JP
- Japan
- Prior art keywords
- filter medium
- specimen
- reagent
- absorbs
- porous film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004458 analytical method Methods 0.000 title claims abstract description 14
- 238000001035 drying Methods 0.000 title 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 11
- 239000000463 material Substances 0.000 claims abstract description 6
- 230000035945 sensitivity Effects 0.000 claims abstract description 3
- 238000012360 testing method Methods 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 12
- 239000007795 chemical reaction product Substances 0.000 claims description 4
- 239000012085 test solution Substances 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims 1
- 230000003100 immobilizing effect Effects 0.000 claims 1
- 239000012528 membrane Substances 0.000 abstract description 4
- 239000011148 porous material Substances 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 abstract description 3
- 239000000020 Nitrocellulose Substances 0.000 abstract description 2
- 239000004677 Nylon Substances 0.000 abstract description 2
- 229920001220 nitrocellulos Polymers 0.000 abstract description 2
- 229920001778 nylon Polymers 0.000 abstract description 2
- 238000010438 heat treatment Methods 0.000 abstract 1
- 239000002250 absorbent Substances 0.000 description 4
- 230000002745 absorbent Effects 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229920006316 polyvinylpyrrolidine Polymers 0.000 description 3
- 238000011896 sensitive detection Methods 0.000 description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000004094 surface-active agent Substances 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- 102000008100 Human Serum Albumin Human genes 0.000 description 2
- 108091006905 Human Serum Albumin Proteins 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000001509 sodium citrate Substances 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 230000002485 urinary effect Effects 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 239000004695 Polyether sulfone Substances 0.000 description 1
- QPMIVFWZGPTDPN-UHFFFAOYSA-N Tetrabromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C(C(Br)=C(Br)C(Br)=C2Br)=C2S(=O)(=O)O1 QPMIVFWZGPTDPN-UHFFFAOYSA-N 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- SMEGJBVQLJJKKX-HOTMZDKISA-N [(2R,3S,4S,5R,6R)-5-acetyloxy-3,4,6-trihydroxyoxan-2-yl]methyl acetate Chemical compound CC(=O)OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O1)O)OC(=O)C)O)O SMEGJBVQLJJKKX-HOTMZDKISA-N 0.000 description 1
- 229940081735 acetylcellulose Drugs 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920006393 polyether sulfone Polymers 0.000 description 1
- -1 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical group FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は、例えば病気の発見・診療等に際して尿などの
検液中に含有される各種成分の有無及び量を検査するた
めの高感度分析片に関するものである。Detailed Description of the Invention (Industrial Application Field) The present invention is a highly sensitive analysis method for testing the presence and amount of various components contained in test fluids such as urine, for example, in the discovery and treatment of diseases, etc. It's about pieces.
（従来の技術）
例えば病気の発見・診察に際し、尿などの検液中に含有
される各種成分の有無及び量を知ることは極めて重要で
ある。(Prior Art) For example, when discovering or diagnosing a disease, it is extremely important to know the presence or absence and amount of various components contained in a test fluid such as urine.
従来このような検液検査のための検査体としては、塩化
ビニール等のプラスチックからなる支持体表面に試薬を
含浸させたが祇を、両面接着テープ等を使って接着し成
形した試験紙タイプのものが、その簡便さゆえに多用さ
れている。しかしこのような試験紙タイプのものにも、
おのずと検出限界があるため、さらに高感度の検出を行
う場合は、ＲＩＡ法、ラテックス凝集反応法等の如く、
より煩雑な検査が必要とされている。Conventionally, test specimens for such liquid test tests have been made by impregnating the surface of a support made of plastic such as vinyl chloride with reagents, but test paper-type specimens made by adhering and molding using double-sided adhesive tape or the like have been used. It is widely used because of its simplicity. However, even with this type of test paper,
Since there is a detection limit naturally, when performing even more sensitive detection, RIA method, latex agglutination reaction method, etc.
More complicated tests are needed.
（発明が解決しようとする課題）
本発明は検液中の各種成分について高感度の検出を簡便
に実施し得る試験紙タイプの分析片を提供することを目
的とする。(Problems to be Solved by the Invention) An object of the present invention is to provide a test strip type analysis piece that can easily perform highly sensitive detection of various components in a test solution.
（課題を解決するための手段）
本発明者らは鋭意研究の結果、検液検査用試薬と検液と
の反応生成物が、ある条件下、例えばある一定のｐＨ領
域下で難水溶性である場合において、あらかじめ検液検
査用試薬を吸着・保持もしくは固定化された多孔性フィ
ルム濾材１と、吸水性シート２とを第１図のごとく密着
一体化することにより、スポットされた検液がはじめに
多孔性フィルム濾材１上で検液検査試薬と反応し、その
反応生成物が多孔性フィルム濾材上に残り、過剰の水分
が下の吸水性シートに排除されるため事実上濃縮される
ので、高感度の検出が比較的容易に行われることを見出
した。(Means for Solving the Problems) As a result of intensive research, the present inventors have found that the reaction product between a test liquid test reagent and a test liquid is poorly water-soluble under certain conditions, for example, under a certain pH range. In some cases, by closely integrating the porous film filter medium 1, which has previously adsorbed and retained or immobilized the test reagent, and the water-absorbing sheet 2 as shown in Fig. 1, the spotted test liquid can be absorbed. First, it reacts with the test liquid test reagent on the porous film filter medium 1, and the reaction product remains on the porous film filter medium, and the excess moisture is removed to the water absorbent sheet below, so that it is effectively concentrated. We have found that highly sensitive detection is relatively easy to perform.
すなわち、本発明の要旨は、検液検査用試薬を吸収・保
持せしめるか、もしくは固定化してなる多孔性フィルム
濾材と、検液中の水分を吸収する吸水性シートとを密着
させてなることを特徴とする高感度乾式分析片にある。That is, the gist of the present invention is that a porous film filter material that absorbs and retains or immobilizes a reagent for test liquid and a water-absorbing sheet that absorbs moisture in the test liquid are brought into close contact with each other. The feature lies in the highly sensitive dry analysis piece.
本発明において、多孔性フィルム濾材とは、いわゆるメ
ンブランフィルタ−（孔径０．１〜１０ｎ）、ウルトラ
フィルター（孔径１０〜１０００人）等を言い、また基
材はニトロセルロース、アセチルセルロース、ナイロン
、ポリカーボネート、ポリエーテルサルホン、ポリテト
ラフルオロエチレン（四弗化エチレン樹脂）等があり、
いずれも市販の製品を使用できる。In the present invention, porous film filter media refers to so-called membrane filters (pore size 0.1 to 10 nm), ultra filters (pore size 10 to 1000 nm), etc., and the base material is nitrocellulose, acetylcellulose, nylon, polycarbonate. , polyethersulfone, polytetrafluoroethylene (tetrafluoroethylene resin), etc.
Commercially available products can be used in both cases.
この多孔性フィルム濾材は網目構造になっており、検液
と検液検査用試薬との反応生成物を、分別して、その全
てもしくは一部をその表面上に吸着・保持もしくは固定
化して、とどめておく機能を有する。This porous film filter material has a mesh structure, and it separates the reaction products of the test solution and test reagent, and absorbs, retains, or immobilizes all or part of them on its surface, and stores them. It has the function of keeping
この多孔性フィルム濾材に対して密着一体化される吸水
性シートは、充分な吸水性を有するものであることが必
要である。The water absorbent sheet that is closely integrated with the porous film filter medium needs to have sufficient water absorbency.
本発明を更に説明するため実施例を挙げるが、本発明は
これら実施例に限定されるものではない。Examples will be given to further explain the present invention, but the present invention is not limited to these Examples.
実施例１
尿中微量アルブミン測定用分析片について：東洋決紙Ｋ
Ｋ製メンブレンフィルターＡ３００Ａを、下記組成溶液
に浸漬して含浸させ、続いてこのメンブレンフィルター
を取出し、８０’ｃにて３０分間乾燥し、多孔性フィル
ム濾材を得た。Example 1 About the analysis strip for measuring urinary microalbumin: Toyo Keishi K
A membrane filter A300A manufactured by K was immersed in a solution having the composition shown below to be impregnated, and then the membrane filter was taken out and dried at 80'C for 30 minutes to obtain a porous film filter medium.
１次液
テトラブロムフェノールブルー ０．０４ ｇツイン−
６０（界面活性剤） ０．０２ ｇメタノール
５０ｍｆｉ■次液
クエン酸（無水） ９．６ｇクエン
酸ソーダ（２１１２０） ４．５ ｇポリ
ビニルピロリドン（Ｋ ９０） ０．１ ｇ純水
５０ｍ１！次に東洋炉祇
ＫＫ製濾紙Ｎｏ、 １０２７を下記組成溶液中に浸漬し
て含浸させ、続いてか紙を取出し、８０°Ｃにて３０分
間乾燥し、吸水性シートを得た。Primary liquid tetrabromophenol blue 0.04 g Twin-
60 (surfactant) 0.02 g methanol 50 mfi ■Next solution Citric acid (anhydrous) 9.6 g Sodium citrate (21120) 4.5 g Polyvinylpyrrolidone (K 90) 0.1 g Pure water 50 m1! Next, filter paper No. 1027 manufactured by Toyorogi KK was immersed in a solution having the composition shown below to be impregnated, and then the paper was taken out and dried at 80°C for 30 minutes to obtain a water absorbent sheet.
ポリビニルピロリドン（Ｋ ９０） ０．１ ｇ
ツイン−６０（界面活性剤）０．１ｇ
純水 １００ ｍｆｌこのよ
うにして処理された多孔性フィルム濾材と吸水性シート
とを重ね合わせ７０〜８０″Ｃの熱をかけることによっ
てヒートシールさせ一体化させた。Polyvinylpyrrolidone (K90) 0.1 g
Twin-60 (surfactant) 0.1 g Pure water 100 mfl The thus treated porous film filter medium and water absorbent sheet are stacked together and heat-sealed by applying heat of 70 to 80"C to integrate them. I let it happen.
この一体化サンプルをＩｃｍ角に断裁し分析片を得た。This integrated sample was cut into Icm square pieces to obtain analysis pieces.
実施例２
尿中微量アルブミン測定用分析片について：吸水性シー
トの含浸液のみ下記組成に変更し、他は全て実施例Ｉと
同様に処理・一体化して分析片を得た。Example 2 Regarding an analytical piece for measuring urinary microalbumin: Only the impregnating liquid for the water-absorbing sheet was changed to the following composition, and everything else was treated and integrated in the same manner as in Example I to obtain an analytical piece.
ポリビニルピロリドン（Ｋ ９０） ０．５ ｇ
ツイン−６０（界面活性剤） ０．１ ｇク
エン酸（無水） ４．８ｇクエン酸ソー
ダ（２Ｈ２０） ２．２ ｇ純水
１００ｍＩｌ（使用例）
（１）ヒトアルブミン濃度０，３，５．ｌ０ｍｇ／ｄｌ
の４種標準溶液３０ｐ１を実施例１．２の各分析片上に
滴下する。Polyvinylpyrrolidone (K90) 0.5 g
Twin-60 (surfactant) 0.1 g Citric acid (anhydrous) 4.8 g Sodium citrate (2H20) 2.2 g Pure water
100 ml (example of use) (1) Human albumin concentration 0, 3, 5. l0mg/dl
30p1 of the four standard solutions were dropped onto each analysis piece of Example 1.2.
次にこれらが充分浸透後分析片表面の余分な液を洗い流
すためすみやかに蒸留水１００μｌを滴下し、充分浸透
後直ちに表面の呈色を観察した。Next, after these were sufficiently penetrated, 100 μl of distilled water was immediately dropped to wash away the excess liquid on the surface of the analysis piece, and the coloration of the surface was immediately observed after sufficiently penetrated.
その結果、従来の尿中タンパク検査用試験紙（東洋が紙
ＫＫ製ウつビース■蛋白質測定部）と比較すると、下表
のごとく前記実施例１．２の分析片の方がはるかに高感
度であり、アルブミン濃度３ ｍｇ ／ ｄｉ程度の有
無の判定が可能となった。As a result, when compared with the conventional test strip for urine protein testing (Toyoga Paper KK Utsubis protein measuring section), the analytical strip of Example 1.2 has much higher sensitivity as shown in the table below. Therefore, it became possible to determine the presence or absence of an albumin concentration of approximately 3 mg/di.
別できることが判明した。It turns out that they can be separated.
（２）ヒトアルブミン濃度０，３．５■／准の３種標準
溶液にあらかじめｐ）１９．０のＢｕｆｆｅｒを添加し
たものについて、前記使用例１と同様にして表面の呈色
を観察した。その結果下表のごと〈実施例１より実施例
２の方がｐｉ（による影響をうけずに識（発明の効果）
本発明によれば、高感度の検液検査用乾式分析片を提供
することができるので、医療上極めて有用である。(2) A buffer of p) 19.0 was added in advance to three standard solutions of human albumin concentrations of 0 and 3.5 µ/semi, and the coloration on the surface was observed in the same manner as in Use Example 1 above. As a result, as shown in the table below, Example 2 was better than Example 1 because it was not affected by pi (effect of the invention). Therefore, it is extremely useful medically.
第１図は本発明の高感度分析片の構成を示す斜視図であ
る。
１：多孔性フィルム濾材、２：吸水性シートフ孔・Ｈ虹
フィノムムスＰ＃
２二吠水・恩ン一とFIG. 1 is a perspective view showing the structure of a highly sensitive analysis piece of the present invention. 1: Porous film filter medium, 2: Water-absorbing sheet with holes, H Nijifinommus P# 2 Nibosui, Onichi and
Claims (3)
は固定化してなる多孔性フィルム濾材と、検液中の水分
を吸収する吸水性シートとを密着させてなることを特徴
とする高感度乾式分析片。(1) High sensitivity characterized by a porous film filter material that absorbs and retains or immobilizes a test liquid testing reagent and a water-absorbing sheet that absorbs moisture in the test liquid. Dry analysis piece.
の反応生成物を、分別して、その全てもしくは一部をそ
の表面上に吸着・保持もしくは固定化して、とどめられ
ることを特徴とする第１項記載の高感度乾式分析片。(2) The porous film filter material is characterized by being able to separate the reaction products of the test solution and the test solution reagent, and retain all or part of them by adsorbing, retaining, or immobilizing them on its surface. The high-sensitivity dry analysis piece according to item 1.
を有することを特徴とする第１項及び第２項記載の高感
度乾式分析片。(3) The high-sensitivity dry analysis piece according to items 1 and 2, wherein the water-absorbing sheet has sufficient water absorption to absorb water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11285790A JPH0412272A (en) | 1990-04-28 | 1990-04-28 | High-sensitivity dry process analysis piece |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11285790A JPH0412272A (en) | 1990-04-28 | 1990-04-28 | High-sensitivity dry process analysis piece |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0412272A true JPH0412272A (en) | 1992-01-16 |
Family
ID=14597272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11285790A Pending JPH0412272A (en) | 1990-04-28 | 1990-04-28 | High-sensitivity dry process analysis piece |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0412272A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102667479A (en) * | 2009-11-24 | 2012-09-12 | 韩国生命工学研究院 | Membrane biosensor to which a porous film is attached, and a method for measuring immune reactions or enzyme reactions employing the same |
| CN104502343A (en) * | 2015-01-23 | 2015-04-08 | 昆明泊银科技有限公司 | Making method of special dedicated test paper |
-
1990
- 1990-04-28 JP JP11285790A patent/JPH0412272A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102667479A (en) * | 2009-11-24 | 2012-09-12 | 韩国生命工学研究院 | Membrane biosensor to which a porous film is attached, and a method for measuring immune reactions or enzyme reactions employing the same |
| CN104502343A (en) * | 2015-01-23 | 2015-04-08 | 昆明泊银科技有限公司 | Making method of special dedicated test paper |
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