JPH04112730A - Raising method for haploid in cruciferae - Google Patents
Raising method for haploid in cruciferaeInfo
- Publication number
- JPH04112730A JPH04112730A JP2232595A JP23259590A JPH04112730A JP H04112730 A JPH04112730 A JP H04112730A JP 2232595 A JP2232595 A JP 2232595A JP 23259590 A JP23259590 A JP 23259590A JP H04112730 A JPH04112730 A JP H04112730A
- Authority
- JP
- Japan
- Prior art keywords
- inflorescences
- haploid
- temperature treatment
- pollen
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000219193 Brassicaceae Species 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000012258 culturing Methods 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 4
- 241000219198 Brassica Species 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 4
- 240000007124 Brassica oleracea Species 0.000 abstract description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 abstract description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 abstract description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 abstract description 2
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
- 235000013311 vegetables Nutrition 0.000 abstract 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 7
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 5
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 5
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 5
- 241000282373 Panthera pardus Species 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 description 3
- 244000061176 Nicotiana tabacum Species 0.000 description 3
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 3
- 239000007844 bleaching agent Substances 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 210000002242 embryoid body Anatomy 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 229960000367 inositol Drugs 0.000 description 2
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 229940011671 vitamin b6 Drugs 0.000 description 2
- HMUNWXXNJPVALC-UHFFFAOYSA-N 1-[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C(CN1CC2=C(CC1)NN=N2)=O HMUNWXXNJPVALC-UHFFFAOYSA-N 0.000 description 1
- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- 241000208296 Datura Species 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000021186 dishes Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- UDILIGPFQMPPTF-UHFFFAOYSA-K pentapotassium;phosphate Chemical compound [K+].[K+].[K+].[K+].[K+].[O-]P([O-])([O-])=O UDILIGPFQMPPTF-UHFFFAOYSA-K 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000008160 pyridoxine Nutrition 0.000 description 1
- 239000011677 pyridoxine Substances 0.000 description 1
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 1
- 239000011764 pyridoxine hydrochloride Substances 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は、アブラナ科Ｎ物の葯又は花粉を培養して半数
体を得る育成方法において、半数体を効率良く得るため
の育成方法に間する。Detailed Description of the Invention (Industrial Application Field) The present invention relates to a method for cultivating the anthers or pollen of a Brassicaceae species to obtain a haploid. do.
（従来の技術）
一般に、作物育種において５朽又は花粉培養によって半
数体を育成する方法は、短期間に固定品種をつくる方法
として、研究され、一部ですでに実用に供されている。(Prior Art) In general, in crop breeding, the method of growing haploids by 5 rot or pollen culture has been studied as a method for creating fixed varieties in a short period of time, and some have already been put into practical use.
実用に供されている作物としては、イネ、コムギ、タバ
コ、ナス等である。Examples of crops that are put to practical use include rice, wheat, tobacco, and eggplant.
ハクサイにおいては、釣培養による胚状体の作出効率が
、一部の品種においてのみ、実用のレベルに達している
に過ぎず、他の大部分の品種においては、豹又は花粉の
培養による胚状体の作出効率が極めて低い状態であった
。In Chinese cabbage, the efficiency of producing embryos by fishing culture has reached a practical level only in some varieties, and in most other varieties, embryos are produced by culturing leopard or pollen. The body's production efficiency was extremely low.
（発明が解決しようとする課題）
イネ、コムギ、タバコ、ペチュニア、ダチュラ等の作物
においては、花序を切り取った後に、該花序を低温処理
してから、め培養すると、上記胚状体の作出効率が向上
することが知られていた。(Problem to be solved by the invention) In crops such as rice, wheat, tobacco, petunia, datura, etc., after cutting the inflorescences, the inflorescences are treated at low temperature and then cultured. was known to improve.
しかしながら、アブラナ科植物においては、低温処理に
よって胚状体の作出効率が向上せず、むしろ逆に低下す
ることが報告（Ｊｏｕｒｎａｌ ｏｒ Ｅｘｐｅｒｉｍ
ｅｎｔａｌ Ｂｏｔａｎｙ Ｖｏｌ、３６，６７９−６
８９０．１９８５年、９照）されている。However, in Brassicaceae plants, it has been reported that low-temperature treatment does not improve the embryonic production efficiency, but rather decreases it (Journal or Experiment).
ental Botany Vol, 36,679-6
890.1985, 9th edition).
本発明は、従来技術における上記課題を解決せんとする
ものであり、アブラナ科植物の葯又は花粉を培養して、
半数体を効率的に育成する方法を提供することを目的と
する。The present invention aims to solve the above-mentioned problems in the prior art, and involves culturing the anthers or pollen of a Brassicaceae plant,
The purpose is to provide a method for efficiently growing haploids.
（課題を解決するための手段）
本発明は、アブラナ科植物の抽だい後の花序３切り取り
、該花序を水さしした状態で、１〜１０℃の温度範囲に
おいて１〜３日間低温処理した後、該花序より得られた
巧又は花粉と培養することと特徴とするアブラナ科植物
における半数体の育成方法を要旨とするものである。(Means for Solving the Problems) The present invention involves cutting off three bolted inflorescences of a Brassicaceae plant, and subjecting the inflorescences to water in a low temperature range for 1 to 3 days in a temperature range of 1 to 10°C. The gist of this invention is a method for growing haploid plants in Brassicaceae plants, which is characterized by culturing with pollen obtained from the inflorescences.
アブラナ科植物は、例えば、ハクサイ、キャベツ、ブロ
ッコリー、ダイコンなどである。Examples of cruciferous plants include Chinese cabbage, cabbage, broccoli, and radish.
花序を水さしした状態とは、切り取った花序の切り口を
水又は養液水に浸る状態にすることである。The condition in which the inflorescence is placed in water means that the cut end of the cut inflorescence is immersed in water or nutrient solution.
花序を水さしする養液水としては、窒素、リン酸５カリ
などの栄養素を含んだ水であり、例えば、ハイボネック
ス（登録商標）３００〜１０００倍液である。The nutrient water used to water the inflorescences is water containing nutrients such as nitrogen and pentapotassium phosphate, such as Hyvonex (registered trademark) 300 to 1000 times solution.
花序を水さししながら、１〜１０℃の温度範囲において
１〜３日間低温処理を施す。The inflorescences are subjected to a low temperature treatment for 1 to 3 days in a temperature range of 1 to 10°C while being watered.
低温処理後、花序より葯又は花粉を取り出し、殺菌処理
を施した後、培地に置床して培養し、胚状体を作出させ
る。培地としては、修正Ｂ５培地（Ｃａｎａｄｉａｎ
Ｊｏｕｒｎａｌ ｏｒ Ｇｅｎｅ［ｉｃｓ ａ
ｎｄ Ｃｙｔｏｌｏｇｙ１７：６５５−６６６ｐ参照
）、改変ＮＮ培地（育種学会誌３７巻 別冊２ １０８
−１０９ｐ参照）などが用いられる。After the low temperature treatment, anthers or pollen are taken out from the inflorescence, sterilized, placed on a medium, and cultured to produce embryoids. As a medium, modified B5 medium (Canadian
Journal or Gene [ics a
nd Cytology 17:655-666p), modified NN medium (Journal of the Society of Breeding, Vol. 37, Special Issue 2, 108
-109p) etc. are used.
作出された胚状体から常法により半数体を再分化させ、
該半数体を倍化させれば、短期間に固定品種を得ること
ができる。Redifferentiate the haploids from the created embryonic body using a conventional method,
By doubling the haploid, a fixed variety can be obtained in a short period of time.
（作用）
アブラナ科植物の花序を切り取って、水さししながら、
低温処理を施すことによって、朽又は花粉を培養するこ
とによる胚状体の作出効率を向上させることができる。(Action) Cut off the inflorescence of a Brassica plant and add water.
By performing low-temperature treatment, it is possible to improve the efficiency of producing embryonic bodies by culturing rot or pollen.
（実施例）
実施例１
１、供試品種：ハクサイ（Ｂ、 ｃａＢｅｔｒ＋ｓ ）
の「つばめＪ、’５ａｌａｄａ」及び’ Ｆ ｏ ｒ
ｍ ｏ−５ａＪの３品種。(Example) Example 1 1. Test variety: Chinese cabbage (B, caBetr+s)
``Tsubame J, '5alada'' and 'F or
Three varieties of m o-5aJ.
２、供試材料 上記３品種の種子をビニポットに措穫し
、双葉が展開した頃５℃、５００〜１゜０００１ｕｘ連
続照明下で約１か月間低温処理を行い、その後２０℃に
設定Ｌｆ：ｉＡ室（夜間は２３５１ｕｘの照明を行う）
に移し、抽だいさせた。2. Test materials Seeds of the above three varieties were harvested in a plastic pot, and when the futaba developed, they were subjected to low temperature treatment at 5℃ under continuous lighting of 500 to 1゜0001ux for about one month, and then set at 20℃ Lf: iA room (2351ux lighting at night)
and bolted.
３、花序の低温処理：抽だい後の花序を取り、５００倍
のハイボネックス水溶液に水さしした。3. Low-temperature treatment of inflorescences: After bolting, the inflorescences were taken and poured into a 500x Hyvonex aqueous solution.
これを５℃下で１〜３日間低温処理を加えた。This was subjected to low temperature treatment at 5° C. for 1 to 3 days.
４、花粉培ｉＩ＝上記低温処理を加えた花序より、幅１
２〜２．０−の蕾を集め、０１％才スパン５秒、１％さ
らし粉で１０分間表面殺菌を行い、滅菌水で２回洗浄す
る。その後、蕾を１３％５ｕ−ｃｒｏｓｅを加えた修正
Ｂ５液体培地中で、注射筒を用いて押しつぶし、花粉を
遊離させる。これを４０μｍのナイロンメツシュで濾過
し、１０１００Ｘ分で３回遠心洗浄後、改変ＮＮ培地中
にＩＸ １０ ’ ／ ｍｌの密度に調整し、６ａａ径
のシャーレに１．５ｍｌずつ分注した。使用した修正Ｂ
５液体培地及び改変ＮＮ培地の組成は、次の通りである
。4. Pollen culture iI = width 1 from the inflorescence treated with the above low temperature treatment
Collect buds of 2 to 2.0, sterilize the surface with 1% bleaching powder for 5 seconds and 10 minutes with 1% bleaching powder, and wash twice with sterile water. Thereafter, the buds are crushed using a syringe in a modified B5 liquid medium supplemented with 13% 5u-crose to release pollen. This was filtered through a 40 μm nylon mesh, centrifuged and washed three times at 10,100× minutes, adjusted to a density of IX 10′/ml in a modified NN medium, and dispensed in 1.5 ml portions into 6 aa diameter petri dishes. Modification B used
The compositions of the 5 liquid medium and the modified NN medium are as follows.
修正Ｂ５培地の組成
ＮａＨ，ＰＯ４＊Ｈ，Ｏ１５０
ｆＮＨ，）２ＳＯ４１３４
ＣａＣ１２−２Ｈ，０７５０
Ｈａ、 ＥＤＴＡ ３７．３
２ｎＳＯ，＊７Ｈ２０２
ＣｏＣ１□市６Ｈ２００，０２５
Ｈ，８０゜
Ｉｎｏｓｉｔｏｌ ＴＯＯ
Ｐｙｒｉｄｏｘｉｎｅ＊Ｉ（ＣＩ １．０Ｇｌｕｔａｍ
ｉｎｅ ８０Ｏ
ＮＡＡ Ｏ，＋
５ｕＣｒＯ３ｅ １Ｇ％
ｐＨ５，７〜５８
（■／Ｊ ）
Ｎ０ｊ
ＨＱＳＯ４−Ｈ□０
ＦｅＳＯ，＊７Ｈ，Ｏ
ＨｎＳ０４ｍ）ｌ□０
ＣｕＳＯｔ＊５Ｈ２０
に＋
Ｈａ、 ）１００．８２日、 Ｏ
Ｎ＋ＣＯｔ＋ｎＩＣａＣ
ＴｈｉａＩＩＩｉｎｎｅ＊）ＩＣＩ
ｅｒｅｎｅ
４−Ｄ
Ａｇａｒｏｓｅ
（以下余白）
ＬＯ
０，８Ｘ
（以下余白）
改変ＮＮ培地の組成
ＨｇＳ Ｏ，本７Ｈ２０４２５
Ｃａ（Ｎｏ３）２ｍａＨ，０５００
ＦｅＳＯ，−７Ｈ２０２７，８５
ＨｎＳＯ，−４Ｈ，０２２，３
ＣｕＳＯ，＊５）１２０ ０．０２５
Ｈ，８０３６，２
に１０８３
Ｉｎｏｓｉｔｏｌ １００
Ｐｙｒｉｄｏｘｉｎｅ傘ＨＣｌ Ｏ，５Ｂｉｏｔｉｎ
Ｏ，０５
Ｆｏｌｉｃ ａｃｉｄ ０．５
し−ｇｌｕｔａｍｉｎｓ ８０ＯＮＡＡ
Ｏ，５
ＳＵｇａ ｒ １３％
ｐＨ６，０
（■／ｆＪ ）
ＮＯ３
Ｈ２ＰＯ４
Ｈａ２ＥＤＴ＾
１ｎｓＯｔ＊７Ｈ２０
ＣｏＣ１２傘６Ｈ２０
Ｈａ２Ｈｏｅ、 傘２Ｈ，’０
ＮＩＣＯｔｌｎｌＣａＣ
Ｔｈｉａｍｉｎｎｅ＊ＨＣＩ
Ｇｌｙｓ＋ｎｅ
ｃ ｕｔａｔ＋ｏｎｅ
Ｌ−５ｅｒｌｎｅ
Ａ
（以下余白）
花粉培養は、３５°Ｃ暗黒下で１日間高温処理し。Composition of modified B5 medium NaH, PO4*H, O150 fNH,)2SO4134 CaC12-2H, 0750 Ha, EDTA 37.3 2nSO, *7H202 CoC1□City 6H200,025 H, 80° Inositol TOO Pyridoxine* I (CI 1. 0Glutam
ine 80O NAA O,+5uCrO3e 1G% pH5,7~58 (■/J) N0j HQSO4-H□0 FeSO,*7H,O HnS04m)l□0 CuSOt*5H20 + Ha, )100.82 days, O N+COt+nICaC ThiaIIIinne*) ICI erene 4-D Agarose (blank below) LO 0,8X (blank below) Composition of modified NN medium HgSO, book 7H20425 Ca (No3) 2maH, 0500 FeSO, -7H2027,85 HnSO ,-4H, 022,3 CuSO, *5) 120 0.025 H, 8036,2 to 1083 Inositol 100 Pyridoxine HCl O,5Biotin
O,05 Folic acid 0.5 Shi-glutamins 80ONAA
O,5 SUgar 13% pH6,0 (■/fJ) NO3 H2PO4 Ha2EDT^ 1nsOt*7H20 CoC12 Umbrella 6H20 Ha2Hoe, Umbrella 2H,'0 NICOtlnlCaC Thiaminne*HCI Glys+ne cut at+one L-5erlne A (blank below) Pollen culture is , high temperature treatment at 35°C for 1 day in the dark.
以後２５℃暗黒条件にして行った。１か月後における、
１シヤーレ当たりの胚状体数を調査した。Thereafter, the test was carried out at 25° C. in the dark. After one month,
The number of embryoids per share was investigated.
その結果を第１表に示す。The results are shown in Table 1.
第１表 注 １）抽だいの低温処理を行わずに培養したもの。Table 1 Note 1) Cultured without bolting at low temperature.
２）本発明区胚状体発生数／対照区胚状体発生数
実施例２
１、供試品種、ハクサイ（Ｂ、 ｃａｍｐｅｔｒｉｓ）
のｒｓａ ｌ ａｄａ」品種。2) Number of embryoids generated in the invention area/number of embryoids generated in the control area Example 2 1. Test variety: Chinese cabbage (B, campetris)
'rsa lada' variety.
２、供試材料：実施例１と同じ。2. Test material: Same as Example 1.
３、花序の低温処理二抽だい後の花序を取り、養分を含
まない水に水さしした。これを５℃下で３日間低温処理
を行った。3. Low-temperature treatment of inflorescences After bolting, the inflorescences were taken and poured into nutrient-free water. This was subjected to low temperature treatment at 5° C. for 3 days.
４、花粉培養：実施例１と同じ。4. Pollen culture: Same as Example 1.
その結果を第２表に示す。The results are shown in Table 2.
第２表
実施例３
１、供試品種：ハクサイ（Ｂ、Ｃａ１ｐｅｔｒｉＳ ’
）の「つばめ」品種。Table 2 Example 3 1. Test variety: Chinese cabbage (B, Ca1petriS'
) 'Tsubame' variety.
２、供試材料・上記品種の種子をビニボ・ｌトに播種し
、双葉が展開した頃５℃、５００〜１，０００１ｕｘ連
続照明下で約１か月間低温処理を行い、その後２０℃に
設定した温室（夜間は、３５１ｕｘの照明を行う）に移
し、抽だいさせた。2. Test materials: Seeds of the above varieties were sown in a binibo-lto, and when the leaves had developed, low temperature treatment was performed at 5℃ under continuous lighting of 500 to 1,0001 ux for about 1 month, and then the temperature was set to 20℃. The plants were transferred to a greenhouse (lighted at 351 ux at night) and bolted.
３、花序の低温処理二抽だい後の花序を取り、５００倍
のハイボネックス水溶液に水さしする。3. Low-temperature treatment of inflorescences Take the inflorescences after second bolting and add them to a 500x Hyvonex aqueous solution.
これを５℃下で３日間低温処理を加えた。This was subjected to low temperature treatment at 5° C. for 3 days.
対照区については、切り取った花序を養液水に水さしせ
ずに、プラスチックのケースに入れて。For the control plot, the cut inflorescences were placed in a plastic case without being soaked in nutrient solution.
同様の低温処理を行った。A similar low temperature treatment was performed.
４、花粉培ｇ＃二上記低温処理を加えた花序より、幅１
２〜２．０−の蕾を集め、０．１％才スパン５秒、１％
さらし粉で１０分間表面殺菌を行い、滅菌水で２回洗浄
する。その後、蕾より豹を取り出して、修正Ｂ５培地（
組成は、実施例１と同じ。4. Pollen culture g #2 From the inflorescences subjected to the above low temperature treatment, width 1
Collect buds of 2 to 2.0-, 0.1% growth span for 5 seconds, 1%
Surface sterilize with bleaching powder for 10 minutes and wash twice with sterile water. After that, the leopard was taken out from the bud, and the modified B5 medium (
The composition is the same as in Example 1.
）に置床した。 豹培養は、３５℃暗黒下で１日間高温
処理し、以後２５℃暗黒条件にして行った。). Leopard culture was carried out at high temperature for 1 day in the dark at 35°C, and then in the dark at 25°C.
１か月後における胚状体の発生数を調査した。The number of embryoid bodies developed one month later was investigated.
その結果を第３表に示す。The results are shown in Table 3.
第３表 注 １）対照区の胚状体数を１とした指数。Table 3 Note 1) Index with the number of embryoid bodies in the control group as 1.
（発明の効果）
本発明の半数体育成方法によって、アブラナ科植物の各
品種について、花粉又は豹を培養して胚状体を得る効率
を向上させることができる。従って、アブラナ科植物に
おける半数体育成方法の実用性を増大するものである。(Effects of the Invention) The haploid breeding method of the present invention can improve the efficiency of culturing pollen or leopard to obtain embryoids for each variety of Brassicaceae plants. Therefore, it increases the practicality of the haploid cultivation method in Brassicaceae plants.
特許出願人 日本たばこ産業株式会社Patent applicant: Japan Tobacco Inc.
Claims (1)
水さしした状態で、１〜１０℃の温度範囲において１〜
３日間低温処理した後、該花序より得られた葯又は花粉
を培養することを特徴とするアブラナ科植物における半
数体の育成方法。Cut the bolted inflorescence of a Brassica plant and place the inflorescence in water for 1 to 10 minutes at a temperature of 1 to 10℃.
A method for cultivating haploid plants in Brassicaceae plants, which comprises culturing anthers or pollen obtained from the inflorescences after low-temperature treatment for 3 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2232595A JP2793896B2 (en) | 1990-09-04 | 1990-09-04 | A method for growing haploids in cruciferous plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2232595A JP2793896B2 (en) | 1990-09-04 | 1990-09-04 | A method for growing haploids in cruciferous plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04112730A true JPH04112730A (en) | 1992-04-14 |
| JP2793896B2 JP2793896B2 (en) | 1998-09-03 |
Family
ID=16941825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2232595A Expired - Lifetime JP2793896B2 (en) | 1990-09-04 | 1990-09-04 | A method for growing haploids in cruciferous plants |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2793896B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113287513A (en) * | 2021-07-01 | 2021-08-24 | 金陵科技学院 | Eggplant haploid plant sexual propagation doubling method based on improvement of pollen activity |
-
1990
- 1990-09-04 JP JP2232595A patent/JP2793896B2/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113287513A (en) * | 2021-07-01 | 2021-08-24 | 金陵科技学院 | Eggplant haploid plant sexual propagation doubling method based on improvement of pollen activity |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2793896B2 (en) | 1998-09-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109258460A (en) | Micro-stem tip culture combines the breeding method of heat treatment acquisition Zengcheng honey chrysanthemum detoxic seedling | |
| CN101548647B (en) | Tree peony callus induction method | |
| CN105993956A (en) | Fast propagating method for atractylis lancea | |
| JP2700741B2 (en) | Cultivation method of moss using cultured species | |
| CN105475139B (en) | A kind of tissue culture propagation method of mao of leaf venushair fern | |
| CN104273027B (en) | Aseptic germination method of Crassulaceae plant seeds | |
| CN108018255A (en) | A kind of suspension culture method of pomegranate cell | |
| CN104322370A (en) | Bitter gourd in vitro rapid propagation method | |
| Daneshvar-Royandezagh et al. | In Vitro micropropagation of garden thyme (Thymbra Spicata L. Var. Spicata L.) collected from southeastern turkey using cotyledon node | |
| CN110604060B (en) | Method for obtaining regeneration plant by in vitro culture of Zhejiang red camellia anther | |
| CN102232359B (en) | In-vitro rapid propagation method of double-petal Jasminum sambac | |
| CN107593446A (en) | A kind of fast breeding method of the induction and regeneration of tree peony | |
| CN105432466B (en) | Method with plant regeneration occurs for a kind of pittosporum tobira somatic embryo | |
| CN108967196B (en) | Culture method of in-vitro microspore regeneration plant of rape | |
| CN107667865B (en) | A kind of Ming River ceratostigma plumbaginoides Bunge subculture quick-breeding method | |
| CN101032224B (en) | Tissue cultivating and seedling method of fast growing anthocephalus | |
| CN114600772B (en) | Tissue culture method and rapid propagation method of michelia figo in remote mountains | |
| CN103535279B (en) | Rooting method for un-rooted tissue culture seedlings of salix matsudana var.tortuosa | |
| KR101887221B1 (en) | Method of mass propagation of bamboo by in vitro culture | |
| CN105830926B (en) | A kind of quick breeding method for tissue culture of witchweed | |
| JPH04112730A (en) | Raising method for haploid in cruciferae | |
| CN102934610A (en) | Breeding maintaining method of ricinus communist female plant | |
| CN107047317A (en) | A kind of Orychophragmus violaceus embryoid and the cultural method of plant | |
| KR101963644B1 (en) | Method of Cultivating Youngia denticulate using Plant Tissue Culture Techniques and Cosmetic Composition containing an Extract from Callus or Suspension Culture | |
| AU2021102670A4 (en) | Method for Tissue Culture and Rapid Reproduction of Tall Fescue (Tall fescue) |