CN101032224B - Tissue cultivating and seedling method of fast growing anthocephalus - Google Patents
Tissue cultivating and seedling method of fast growing anthocephalus Download PDFInfo
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- CN101032224B CN101032224B CN2006100373411A CN200610037341A CN101032224B CN 101032224 B CN101032224 B CN 101032224B CN 2006100373411 A CN2006100373411 A CN 2006100373411A CN 200610037341 A CN200610037341 A CN 200610037341A CN 101032224 B CN101032224 B CN 101032224B
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Abstract
The present invention discloses a tissue culture process for raising seedling of fast growing Anthocephalus chinensis. The tissue culture process includes the following steps: taking bud stick as explant, sterilizing and cleaning; inducing culturing in inducing culture medium comprising 1/2MS, 6-BA 0.8-1.0 mg/L and NAA 0.2-0.3 mg/L at illumination intensity of 1000+/-500 Lux; secondary culturing in secondary culture medium comprising MS, 6-BA 0.3-0.5 mg/L and NAA 0.1-0.2 mg/L at illumination intensity of 2500-3000 Lux; rooting culturing in rooting culture medium comprising MS and NAA 0.1-0.3 mg/L at illumination intensity of 2500-3500 Lux for about 25 days to obtain bottle seedling of 3-4 cm height and 0.5-1.0 cm root length; and transplanting to mixed medium comprising vermiculite and turfin the volume ratio of 2 to 3. The process has culture period of 120 days, survival rate over 96 % and low production cost, and is suitable for large scale production.
Description
Technical field
The invention belongs to biological technical field, specifically refer to the tissue cultivating and seedling method of fast growing anthocephalus.
Background technology
Group's flower (Anthocephalus chinensis) is Rubiaceae a variety of millet fogfruit, is the economic forest seeds that a kind of speed is given birth to, wood property is good, purposes is wide.It is distributed in Sri Lanka, India along the equator through Philippine, Indonesia's one band, China mainly is distributed in ground such as Yunnan, Guangxi, the south of Fujian Province, Fujian, because of its growth is fast, can becomes a useful person in 10 years and liked by the self-employed tree cultivator.
But employing seminal propagation, individual differentiation is big, is difficult to keep a proterties of flower parent; And adopting conventional asexual reproduction methods such as cuttage, press strip or grafting, its survival rate is low, the speed of breeding is slow, is difficult to satisfy the growing market demand.
Plant Tissue Breeding (Tissue culture) is meant the totipotency of utilizing plant cell, (part), tissue and cell exteriorize in the plant corpus, physiological environment in the analogue body, under aseptic, proper temperature and certain condition of culture, make it existence, growth, keeping the method for its normal configuration and function, is a kind of fast asexual propagation technology.
The present invention finds that after deliberation the group's of selection flower choiceness material adopts method for tissue culture, both can keep the merit of parent, can effectively save production cost again, accelerates industrialization and grows seedlings, and this case is arisen spontaneously.
Summary of the invention
The objective of the invention is to provide the tissue cultivating and seedling method of fast growing anthocephalus for the industrially scalable fast breeding.
In order to realize the foregoing invention purpose, technical scheme of the present invention is:
The tissue cultivating and seedling method of fast growing anthocephalus, its step comprise explant sterilization, inducing culture, successive transfer culture, culture of rootage and bottle transplantation of seedlings; Select the rudiment bar as explant, after sterilization, cleaning, insert inducing culture and carry out inducing culture, the inducing culture based formulas is 1/2MS, 6-BA (6-Bian Ji purine) 0.8-1.0mg/L, NAA (methyl) 0.2-0.3mg/L, and intensity of illumination is 1000 ± 500Lux; Insert the medium of successive transfer culture and expanding propagation again, the successive transfer culture based formulas is MS, 6-BA0.3-0.5mg/L, NAA0.1-0.2mg/L, and intensity of illumination is 2500～3000Lux; Enter the culture of rootage stage then, the culture of rootage based formulas is MS, NAA0.1-0.3mg/L, and intensity of illumination is 2500～3500Lux; The bottle seedling was cultivated on root media about 25 days, treated height of seedling 3-4CM, was transplanted to during the long 0.5-1.0CM of root on the mixed-matrix of vermiculite and peat to cultivate, and the ratio of the volume of vermiculite and peat is 2: 3.
When heights of seedling such as above-mentioned bottle transplantation of seedlings are 6-8CM, are transplanted on the matrix of red heart soil, cedar sawdust and baked wheaten cake soil and cultivate, cedar sawdust, red heart soil and to burn the long-pending ratio of the soil body be 4: 5: 1.
The transplantation of seedlings of above-mentioned bottle will irrigate with clear water to mixed-matrix, and obscurity is 70%, and humidity is controlled at 85～90%, and temperature is controlled at 25～30 ℃.
The sterilization of above-mentioned explant, cleaning step are: soaked 20 minutes flushing with clean water half an hour earlier with washing powder solution; With 75% alcohol-pickled 30 seconds, use 0.1%HgCl again
2Sterilized sterile water wash 5-6 time 4 minutes.
After adopting said method, the invention solves vegetative propagations such as seminal propagation and cuttage, press strip or grafting and go out long, problems such as survival rate is low, production cost height of garden cycle, utilization the present invention carries out the going out 120 days garden cycles of tissue cultivating and seedling of fast growing anthocephalus, survival rate is more than 96%, production cost is low, can realize high-quality, speed gives birth to, the fast growing anthocephalus nursery stock industrially scalable fast breeding of resistance.
Embodiment
Be that the specific embodiment that uses the present invention to carry out the tissue cultivating and seedling of fast growing anthocephalus describes in detail below.
A, explant are handled and inducing culture
Select the rudiment bar footpath section of fast growing anthocephalus fine individual plant to do explant, during sterilization, soaked 20 minutes with washing powder solution earlier, flushing with clean water half an hour; With 75% alcohol-pickled 30 seconds, use again
0.1%HgCl
2Sterilized sterile water wash 5-6 time 4 minutes.Induce differentiation culture on the inducing culture being inoculated on the superclean bench, the inducing culture based formulas is: 1/2MS, BA0.8-1.0mg/L, NAA 0.2-0.3mg/L, intensity of illumination is 1000 ± 500Lux.
B, successive transfer culture
At inducing culture about about 30 days, when treating the high 2-3cm of axillalry bud, can enter successive transfer culture and expanding propagation, the successive transfer culture based formulas is MS, 6-BA0.3-0.5mg/L, NAA0.1-0.2mg/L, intensity of illumination is 2500～3000Lux, and the moon growth coefficient of bottle seedling is about 2.5.
C, culture of rootage
Bottle seedling proliferation to be bred is chosen the bottle seedling with certain altitude and is carried out culture of rootage after some, and the culture of rootage based formulas is MS, NAA0.1-0.3mg/L, and intensity of illumination is 2500～3500Lux.
D, take root transplantation of seedlings and management
When the bottle seedling was cultivated on root media about 25 days, treat height of seedling 3-4CM, the long 0.5-1.0CM of root, rooting rate reaches 80% when above, promptly can move on to the booth hardening, and hardening is the transition before transplanting, through hardening about one week, the bottle transplantation of seedlings is cultivated in the mixed-matrix that adds peat through the vermiculite of sterilizing, and the ratio of the volume of vermiculite and peat is 2: 3, covers with insulation, preserves moisture with film.For reducing production costs, when height of seedling is 6-8CM, is transplanted on the matrix of red heart soil, cedar sawdust and baked wheaten cake soil through sterilization and cultivates, cedar sawdust, red heart soil and to burn the long-pending ratio of the soil body be 4: 5: 1.The bottle transplantation of seedlings will irrigate with clear water to mixed-matrix, and obscurity is 70%, and humidity is controlled at 85～90%, temperature is controlled at 25～30 ℃, and spray medicine week about once, take place, use in turn with flolimat, dichlorvos, three kinds of medicines of chlorophos to prevent damage by disease and insect.Begin growth after bottle one week of transplantation of seedlings, the management in later stage is identical with general nursery stock.Transplant after 60 days, height of seedling is 10-30CM, and survival rate reaches the requirement in garden greater than 96%.
Claims (4)
1. the tissue cultivating and seedling method of fast growing anthocephalus is characterized in that: step comprises explant sterilization, inducing culture, successive transfer culture, culture of rootage and bottle transplantation of seedlings;
Select the rudiment bar as explant, after sterilization, cleaning, insert inducing culture and carry out inducing culture, the inducing culture based formulas is 1/2MS, 6-BA0.8-1.0mg/L and NAA0.2-0.3mg/L, and intensity of illumination is 1000 ± 500Lux;
Insert the medium of successive transfer culture and expanding propagation again, the successive transfer culture based formulas is MS, 6-BA0.3-0.5mg/L and NAA0.1-0.2mg/L, and intensity of illumination is 2500～3000Lux;
Enter the culture of rootage stage then, the culture of rootage based formulas is MS and NAA0.1-0.3mg/L, and intensity of illumination is 2500～3500Lux;
The bottle seedling was cultivated on root media 25 days, treated height of seedling 3-4CM, was transplanted to during the long 0.5-1.0CM of root on the mixed-matrix of vermiculite and peat to cultivate, and the ratio of the volume of vermiculite and peat is 2: 3.
2. the tissue cultivating and seedling method of fast growing anthocephalus according to claim 1, it is characterized in that: when heights of seedling such as bottle transplantation of seedlings are 6-8CM, be transplanted on the matrix of red heart soil, cedar sawdust and baked wheaten cake soil and cultivate, cedar sawdust, red heart soil and to burn the long-pending ratio of the soil body be 4: 5: 1.
3. the tissue cultivating and seedling method of fast growing anthocephalus according to claim 1, it is characterized in that: a bottle transplantation of seedlings will irrigate with clear water to mixed-matrix, and obscurity is 70%, and humidity is controlled at 85～90%, and temperature is controlled at 25～30 ℃.
4. the tissue cultivating and seedling method of fast growing anthocephalus according to claim 1, it is characterized in that: explant sterilization, cleaning step are: soaked 20 minutes flushing with clean water half an hour earlier with washing powder solution; With 75% alcohol-pickled 30 seconds, use 0.1%HgCl again
2Sterilized sterile water wash 5-6 time 4 minutes.
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| CN2006100373411A CN101032224B (en) | 2006-08-24 | 2006-08-24 | Tissue cultivating and seedling method of fast growing anthocephalus |
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| CN2006100373411A CN101032224B (en) | 2006-08-24 | 2006-08-24 | Tissue cultivating and seedling method of fast growing anthocephalus |
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| CN101032224A CN101032224A (en) | 2007-09-12 |
| CN101032224B true CN101032224B (en) | 2011-04-20 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104429947B (en) * | 2014-11-13 | 2017-01-11 | 郑州师范学院 | Tissue culture medium composition for adina rubella and rapid tissue culture method of adina rubella |
| CN105638025B (en) * | 2016-01-07 | 2018-12-11 | 广西壮族自治区药用植物园 | A method of rolling into a ball flower nursery |
| CN106171993B (en) * | 2016-07-15 | 2019-01-08 | 华南农业大学 | It is a kind of using a variety of millet wood blade as the highly efficient regeneration method of explant |
| CN108243959B (en) * | 2018-01-30 | 2021-08-24 | 华南农业大学 | Efficient regeneration method taking stem section of sorghum as explant |
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2006
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Non-Patent Citations (4)
| Title |
|---|
| 任盘宇等.热带速生树种团花的造林技术.林业实用技术 6.2004,(6),6-8. |
| 任盘宇等.热带速生树种团花的造林技术.林业实用技术 6.2004,(6),6-8. * |
| 陶永强.团花育苗及造林技术.云南林业26 4.2005,26(4),25-26. |
| 陶永强.团花育苗及造林技术.云南林业26 4.2005,26(4),25-26. * |
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