JPH03980Y2 - - Google Patents

Description

[Detailed Description of the Invention] Background of the Invention [Technical Field] The present invention relates to an animal blood sampling device that can aseptically collect blood from animals, particularly large animals such as cows and horses.

[Prior Art] Blood is collected from animals in order to obtain serum in the blood for the purpose of manufacturing various vaccines, etc.

Conventional methods for collecting blood and obtaining serum from animals are as follows.

A trocar is inserted into the animal's blood vessel, and blood is drawn into a graduated cylinder through a silicone tube connected to the rear end of the needle.

From the top opening of the graduated cylinder containing blood,
If you put a disc-shaped weight with many holes (approximately equal to the inner diameter of the measuring cylinder) wrapped in a cloth filter and leave it for a day or night, the weight will gradually fall due to gravity, and blood clots will form on the underside of the weight. (a combination of fibrin in plasma and blood cell components) settles out,
Serum floats to the top of the weight. In this way, blood clots and serum are separated and only the necessary serum is obtained.

However, in these blood collection methods and serum separation methods, the work is performed while the blood is in contact with the outside air, and therefore cannot be performed aseptically.

Originally, the collection of blood from a human body and the acquisition of serum from that blood are carried out under strict hygiene and safety controls, as the targets for which vaccines, etc. obtained from the serum will be used are humans. In the production of animal vaccines, when blood is collected from animals and serum is collected from the blood, there is little consideration given to hygiene and safety, as the vaccines, etc. obtained from the serum are used on animals. Therefore, it was thought that there was little importance in performing the work aseptically.

However, if dust, bacteria, etc. are mixed into the collected blood or serum, various adverse effects will occur when vaccines obtained from the serum are administered to animals, just as they are to humans.

Therefore, it is necessary to perform the blood collection and serum separation from animals aseptically.

Furthermore, the conventional serum separation method described above also has the disadvantage that it takes a long time to separate serum and blood clots.

Purpose of the invention The purpose of the present invention is to provide an animal blood sampling device that overcomes the above-mentioned drawbacks of the prior art and allows blood sampling from animals to be performed simply, quickly, and aseptically.

DISCLOSURE OF THE INVENTION These objects are achieved by the present invention as described below.

That is, the present invention is an animal blood collection device capable of collecting blood from an animal, which comprises: a flexible tube forming a flow path for the blood collected from the animal to be collected; and a tube at one end of the flexible tube. a connecting device that is attached to the blood collection needle and can be connected to the blood collection needle for fluid communication; and at least one blood ejection means provided on the other end side of the flexible tube, the blood ejection means being pierceable. The present invention provides an animal blood sampling instrument characterized in that the end portion of the tube, which is closed by a partition wall, is covered and sealed with a covering member having an opening means.

The fitting preferably has a hollow male tapered portion and a contamination protection cap overlying the tapered portion.

Further, it is preferable that the connecting device has a fixing means for fixing the connected blood collection needle.

Preferably, the fixing means and the blood collection needle are fixed by screwing.

Preferably, the unsealing means is configured to expose the end portion of the tube by breaking a breakable member that is breakable from the envelope member.

Preferably, a plurality of blood discharge means are connected to the other end of the flexible tube via branch pipes.

Also, the flexible tube is preferably made of soft vinyl chloride.

Specific Structure of the Invention Hereinafter, preferred embodiments of the animal blood sampling device of the present invention shown in the accompanying drawings will be described in detail.

FIG. 1 is a partial sectional view of an animal blood sampling device 1 of the present invention. As shown in the figure, a connector 4 is attached to one end (lower side of the drawing) of the flexible tube 2 via a connecting pipe 3. This connector 4 is connected to a blood collection needle 11, which will be described later, so as to be able to communicate fluidly therewith. Therefore, to ensure that the connection is made,
The tip end 5 of the connector 4 is preferably shaped to have a male hollow tapered portion, a so-called luer taper (hereinafter referred to as a luer arch shape).

This arch-shaped distal end 5 is covered with a contamination prevention cap 10 except when blood is being collected to prevent contamination of the distal end 5 and to maintain the inside of the blood flow path of the blood sampling instrument 1 in a sterile state.

The connecting device 4 also has a blood sampling needle 11 at its tip 5.
It is preferable to have a fixing member 6 as a means for fixing the hub 12 when it is connected.

As shown in FIGS. 1 and 3, this fixing member 6 is an annular member having an outer circumference 7 on the outside of the tip 5, and a spiral thread 8 on the inside of the outer circumference 7. It is locked to the connecting tool 4 at the locking part 9, and is loosely fitted rotatably to the connecting tool 4 without coming off from the connecting tool 4.

As shown in FIG. 2, for example, the blood collection needle 11 includes a transparent or translucent hub 15, an inner needle 16 fixed to the distal end of the hub 15 so as to communicate with the inner lumen of the hub 15, and a hub 12 that is attached to the hub and has a flange 13 at the base end;
The indwelling needle 14 is fixed to the distal end of the hub 12 so as to communicate with the inner cavity of the hub 12, and an inner needle 16 is inserted therethrough in close contact with the indwelling needle 14. The hub 15 to which the inner needle 16 is attached and the hub 12 to which the indwelling needle 14 is attached are detachable.

The indwelling needle 14 is preferably made of a biocompatible material (eg, fluororesin) that has enough strength to be pinched and closed with fingers or the like.

A ventilation filter 17 is provided at the base end of the hub 15.
is installed. As the air-permeable filter 17, it is preferable to use a filter that allows air to pass through but not liquid, that is, blood.
For example, a membrane filter made of a polyester resin base material coated with polyvinyl chloride can be used. By using such a breathable filter, puncture into an animal's blood vessel can be confirmed without leakage of blood from the filter.

To connect the blood sampling needle 11 having such a configuration to the blood sampling device of the present invention, the hub 15 is separated from the hub 12 (the inner needle 16 is removed from the indwelling needle 14), and the tip of the connecting device is connected as shown in FIG. The portion 5 is fitted into the inner cavity of the hub 12 for connection. Since the inner peripheral surface of the hub 12 has a taper approximately equal to the Luer taper of the outer periphery of the tip 5, the fitting connection between the tip 5 and the hub 12 is completed, and no blood leaks from the connection. do not have.

Furthermore, in the present invention, in order to prevent an accident in which the hub 12 connected to the distal end 5 comes off during blood collection, it is preferable to provide an arbitrary fixing means, and as a preferred example thereof, as shown in FIG. The hub 12 can be completely fixed by providing the fixing member 6 and screwing the thread 8 of the fixing member 6 into a flange 13 formed at the base end of the hub 12.

Further, another example of the configuration of the blood collection needle is shown in FIG. 4. As shown in the figure, the blood collection needle 11' consists of a catheter side assembly and an inner needle side assembly, and the catheter side assembly has a flexible catheter tube 18 and a catheter hub 19. Catheter tube 18
At least one side hole 26 communicating with the front end of the annular passage 23 in the catheter tube 18 is bored near the tip of the catheter tube 18 .

A branch pipe 24 is obliquely bored in the catheter hub 19 at approximately the center thereof, and the annular passage 23 communicates with the inner lumen of the branch pipe 24 .
A connector portion 25 is provided at the open end of this branch pipe 24.
is formed, and one end of a transparent or translucent soft tube 27 is connected to this. A connector 28 is connected to the other end of the soft tube 27, and an air-permeable leak-proof thrombus 30 equipped with an air-permeable filter 29 similar to that described above is detachably attached to the open end of the connector 28.

A sealing cap 33 made of an elastic material such as rubber is removably fitted into the proximal opening of the catheter hub 19 . This sealing cap 33 is provided with an insertion hole 35 through which the outer peripheral surface of the inner needle 34 can be inserted in close contact with the inner needle 34.
When the inner needle 34 is removed, the cap closes due to its own elastic force.

On the other hand, the inner needle side assembly consists of a transparent or translucent hub 38 attached to the rear end of the inner needle 34, and the lumen of the hub 38 communicates with the inner needle 34, and the rear end opening is A breathable anti-leakage clot 39 with a breathable filter 29 similar to that described above is attached.

In order to connect the blood sampling needle 11' having such a configuration to the blood sampling device of the present invention, the air permeable leakage-proof thrombus 30 attached to the connector 28 at the end of the branch tube 24 is removed from the connector 28, and the distal end 5 of the connecting device 4 is connected. is connected by fitting into the inner cavity of the connector 28.

Although two examples of blood sampling needles 11 and 11' have been described above, the configuration of the blood sampling needle to be connected to the animal blood sampling device 1 of the present invention is not limited to these, and any configuration may be used.

As shown in FIG. 1, the animal blood sampling device 1 has at least one blood discharge means 41 on the other end side (upper side in the drawing) of the flexible tube 2. As shown in FIG. below,
The configuration of the blood discharge means 41 will be explained.

The tube 42 is connected to the tube 2 via a branch pipe 45, and the distal end portion 43 of the tube 2 is connected to the partition wall 4, as shown in partial cross section in FIG.
4 is occluded. When blood is collected, a puncture needle (not shown) attached to the end of a tube communicating with the blood bag is inserted into the septum 44 to guide blood into the blood bag, so the septum 44 can be pierced by the puncture needle. It should be made of suitable materials. For example, the tube 42 and the partition wall 44 may be integrally molded from resin such as vinyl chloride.

The end portion 43 of the tube is covered and sealed with a covering member 46 to prevent contamination. As the enveloping member 46, it is preferable to use something that can be easily opened, for example, a bag-shaped one made of vinyl chloride resin or the like. Note that the formation position of the partition 44 that closes the tube 42 is within the covered part of the covering member 46 in the example shown in FIG. 1, but in the present invention, the partition 44 and the covered part of the covering member 46 are The positional relationship is not particularly limited.

Such enveloping member 46 has an unsealing means for unsealing it during use. A preferred example of the unsealing means is one in which a breaking member 47 called a peel tab is bonded to the breaking part (hereinafter referred to as a breaking line) of the envelope member 46 by thermocompression bonding, as shown in FIG. This breakable member 47 has a thermocompression bonded portion 49 formed along the break line, so if you pinch the grip portion 48 with your fingers and peel off the breakage member 47 from the envelope member 46, the thermocompression bonded portion 49 Stress concentration occurs along the thermocompression bonding portion 49, and the envelope member 46 breaks along the thermocompression bonding portion 49.

Therefore, the thermocompression bonding portion 49 may be formed at a position where the tube end portion 43 is exposed when the envelope member 46 is broken.

As another example of the configuration of the unsealing means, as shown in FIG.
It may be configured such that the envelope member is broken from zero. As a result, the enveloping member 4 can be
It is convenient to be able to perform 6 breaks. This cut 50 may be formed at an appropriate position such that the distal end 43 of the tube 42 is exposed when the envelope member 46 is broken.

Note that it is also possible to unseal the envelope member 46 by cutting the distal end of the envelope member 46 with a tool such as scissors without providing such a cut 50.

When the amount of blood to be collected is small, such as when the blood collection device of the present invention is used to collect blood from a relatively small animal, the above-mentioned blood discharge means 41 is attached to the other end of the flexible tube 2.
However, when used to collect blood from medium-sized or large-sized animals, since the amount of blood collected is large, a plurality of (for example, about 2 to 20) blood evacuation means are preferably connected via a branch pipe 45. It is better to set

The flexible tube 2 is preferably made of soft resin, particularly soft vinyl chloride.

The concrete effect of the idea Hereinafter, specific effects of the present invention will be explained.

When the blood collection needle 11 shown in FIG. 2 is integrated, that is, the inner needle 16 is inserted into the indwelling needle 14, and these needles 14 and 16 are punctured into the blood vessel of the animal,
The tip of the inner needle 16 communicates with the inside of the blood vessel, and inside the inner needle 16,
Blood then flows into the hub 15. At this time, the air inside the inner needle 16 and the hub 15 is discharged to the outside by the action of the air-permeable filter 17, and the hub 15 is quickly filled with blood, so that no leakage from the filter 17 occurs. Here, since the hub 15 is transparent or translucent, the inflow of blood into the hub 15 can be visually observed from the outside, and the inner needle 16 and the indwelling needle 1 can be visually observed from the outside.
It can be confirmed that the tube 4 was reliably punctured into the blood vessel.

Next, the hub 15 is detached from the hub 12, that is, the inner needle 16 is pulled out from the indwelling needle 14, and the proximal end of the indwelling needle 14 is pinched with fingers or the like to stop the blood from flowing out. The contamination prevention cap 10 attached to the hub 12 is removed, and the tip 5 of the connector 4 is inserted into the inner cavity of the hub 12. Since the inner circumferential surface of the hub 12 and the outer circumferential surface of the distal end portion 5 have approximately the same taper, both surfaces are in close contact with each other, ensuring a secure connection.
No blood leakage occurs during blood collection.

Furthermore, in order to securely fix the connection between the hub 12 and the connector 4, the fixing member 6 is rotated to thread the thread 8 of the fixing member 6 into the flange 13 of the hub 12.

When the fingers holding the indwelling needle 14 are released in this state, blood is guided from the blood vessel into the tube 2 via the indwelling needle 14, the hub 12, the connector 4, and the connecting tube 3.

On the other end side of the tube 2, the point A shown in FIG.
Allow blood to be directed only to.

Next, the enveloping member 4 of the first blood discharge means 41
Open 6. To open the package, use the grip part 4 of the breaking member 47.
When the breakable member 47 is peeled off from the enveloping member 46 by pinching the breakable member 47 with fingers, stress concentration occurs along the thermocompression bonded portion 49, so the enveloping member 46 ruptures along the thermocompression bonding portion 49, and the breakage portion The tube end portion 43 located nearby is exposed.

Blood bags (e.g. Telflex [Terumo Corporation)
When a puncture needle (not shown) attached to the end of the tube that communicates with the tube (registered trademark) is inserted through the opening of the exposed end portion 43 of the tube and pierces the septum 44, the tube 42 and the inside of the blood bag are communicated with each other. The blood flows in the flexible tube 2 toward the blood discharge means 41 side, and is guided into the blood bag through the first branch pipe 45, point B, the tube 42, the puncture needle and tube on the blood bag side.

When the blood bag (for example, capacity is 600 ml) is filled with blood, blood is collected from the second blood discharge means 41 into another blood bag. The operation is stopped by sandwiching point B and point C with a clamp, and removes the puncture needle on the blood bag side from the first blood discharge means 41 (the puncture needle may remain inserted).
At the same time as removing the clamp that pinches point A, remove the second blood draining means 41 using the same procedure as above.
The envelope member 46 is opened and the puncture needle on the second blood bag side is inserted into the septum 44. As a result, blood is collected into the second blood bag in the same manner as described above.

As many times as necessary by such a procedure, i.e.
Repeat this process until the required amount of blood is collected.

For example, if a total of 2400 ml of blood is to be collected using an animal blood collection device equipped with 10 blood ejection means 41, four blood bags each having a capacity of 600 ml are used and sequentially used up to the fourth blood ejection means. blood sample. In this case, the maximum possible blood collection volume is 6000 ml. It goes without saying that a blood bag with a smaller or larger capacity can also be used.

The blood bag 12 containing the blood thus collected is placed in a centrifuge, and the blood is separated into supernatant serum and precipitated blood clots. In addition, if sodium citrate, anticoagulant, etc. are added to the blood in the blood bag and centrifugation is performed, the liquid component (plasma),
It can be separated into white blood cells, platelets and red blood cells.

Various vaccines (for animals and humans) and the like can be produced from the serum thus obtained.

In the animal blood sampling device 1 of the present invention having the above-described function, when the hub 12 of the blood sampling needle 11 and the tip 5 of the connector 4 are connected, the inner cavity of the hub 12 and the tip 5 are kept in the atmosphere until just before the connection. The structure is such that no blood leaks after connection. Furthermore, in the blood discharging means 41, the end portion 43 of the tube for discharging blood is connected to the enclosing member 4.
6 to prevent contamination, and even if the tube is opened immediately before use, it can be opened quickly by simple means without touching the tube end portion 43 or the like.

Therefore, by using the blood sampling device of the present invention, blood can be collected in a substantially sterile manner, and various harmful effects caused by contamination of the collected blood with various bacteria can be prevented.

Effects of the invention The animal blood sampling device of the invention has the following effects.

(1) When the tip of the connector is made into a male taper (luer taper), the connection with the blood sampling needle is made easy and securely, so that blood leakage does not occur during blood sampling. Furthermore, when the blood sampling needle is provided with a fixing means, particularly a screwing fixing means, the connection between the connecting tool and the blood sampling needle is securely fixed, and accidents such as their coming off during blood sampling are prevented.

Furthermore, if the Luer taper portion of the connector is covered with a contamination prevention cap, the blood flow path of the device of the present invention can be kept sterile until the cap is removed immediately before use.

(2) In the blood discharging means, the end portion of the tube that discharges blood to the blood bag side is covered and sealed with an enveloping member, so that contamination of this portion can be prevented. In addition, if a breakable member is bonded to a predetermined part of the envelope member by thermocompression bonding as an opening means, and the distal end of the tube is exposed by breaking the breakable member, it is possible to easily remove the blood without touching the part from which the blood is drained. Moreover, the envelope member can be opened quickly.

Further, since a pierceable septum is provided at the end of the tube, blood is discharged by piercing the septum with a puncture needle on the blood bag side, so that the work can be performed aseptically and no blood leakage occurs.

(3) When a large number of blood discharging means are arranged in series, it is convenient because the necessary number of blood discharging means can be sequentially used according to the required amount of blood to be collected.

As described above, according to the animal blood sampling device of the present invention, blood can be sampled easily and quickly in a sterile manner, and various harmful effects caused by contamination of the collected blood with various bacteria can be prevented.

Therefore, the sanitary properties and safety of vaccines obtained from animal serum have been improved, and the possibility of using the vaccines not only for animals but also for humans has increased.

[Brief explanation of the drawing]

FIG. 1 is a partially sectional plan view of the animal blood sampling device of the present invention. FIG. 2 is a longitudinal sectional view showing an example of the configuration of a blood collection needle. FIG. 3 is a longitudinal sectional view showing a state in which the hub with an indwelling needle of the blood collection needle is connected and fixed to the connector of the blood collection device of the present invention. FIG. 4 is a longitudinal sectional view showing another example of the configuration of the blood collection needle. FIG. 5 is a partial plan view showing another example of the structure of the blood discharge means of the blood sampling device of the present invention. Explanation of symbols, 1...Animal blood sampling device, 2...Flexible tube, 3...Connecting tube, 4...Connecting tool, 5
... Tip part, 6 ... Fixing member, 7 ... Outer circumference part, 8
...screw thread, 9 ... locking part, 10 ... contamination prevention cap, 11, 11' ... blood collection needle, 12 ... hub,
13...flange, 14...indwelling needle, 15...hub, 16...inner needle, 17...breathable filter,
18... Catheter tube, 19... Catheter hub, 23... Annular passage, 24... Branch pipe, 2
5... Connector portion, 26... Side hole, 27... Soft tube, 28... Connector, 29... Breathable filter, 30... Breathable leak-proof thrombus, 33...
... sealing cap, 34 ... inner needle, 35 ... insertion hole, 38 ... hub, 39 ... breathable leak-proof thrombus,
41...Blood evacuation means, 42...Tube, 43
...Tube end portion, 44...Bulkhead, 45...
Branch pipe, 46... Enveloping member, 47... Breaking member,
48...Gripping part, 49...Thermocompression bonding part, 50...Notch.

Claims (1)

[Scope of Claim for Utility Model Registration] (1) An animal blood sampling device capable of collecting blood from an animal, comprising a flexible tube constituting a flow path for the blood collected from the animal to be collected, and the flexible tube. a connector attached to one end of the flexible tube and capable of fluid communication when connected to a blood collection needle; and at least one blood discharge means provided at the other end of the flexible tube, the blood discharge means An animal blood collection device characterized in that the end portion of a tube closed by a penetrable septum is covered and sealed with a covering member having an opening means. (2) The animal blood sampling device according to claim 1, wherein the connector has a hollow male tapered portion and a contamination prevention cap that covers the tapered portion. (3) The animal blood sampling device according to claim 1 or 2, wherein the connecting device has a fixing means for fixing the connected blood sampling needle. (4) The animal blood sampling device according to claim 3, wherein the fixing means and the blood sampling needle are fixed by screwing. (5) According to any one of claims 1 to 4, the unsealing means is configured to expose the end portion of the tube by breaking a breakable member that is breakable from the envelope member. Veterinary blood collection equipment as described. (6) The animal blood sampling device according to any one of claims 1 to 5, wherein a plurality of the blood discharge means are connected via branch pipes. (7) The animal blood sampling device according to any one of claims 1 to 6, wherein the flexible tube is made of soft vinyl chloride.