JPH0360065B2 - - Google Patents

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a biochemical analyzer, specifically, a biochemical analyzer that supplies a test sample to the element surface of an analysis slide in which an element impregnated with a reaction reagent is sandwiched between mounts. The present invention relates to an apparatus for chemically analyzing a change in color density and the like by measuring a change in color density due to a reaction with the test sample.

[Conventional technology]

A conventional biochemical analyzer is shown in FIG. In the figure, 1 is the main body, and 2 is an intermittent rotating body. The intermittent rotating body 2 has a plurality of through holes (not shown) for fitting an analysis slide arranged at equal positions on its peripheral edge 2a, and rotates intermittently at each installation angle of the through holes. around it, there is a supply section 3 for fitting an analysis slide (not shown) into the through hole when stopped;
The dispensing unit 4 drips the test sample onto the element surface of the analysis slide after it has been fitted into each through hole, and the progress state or result of the reaction of the element due to the drop of the test sample is measured, and the color density change due to the reaction is measured. It is equipped with a photometry section 5 that performs measurement and chemical analysis. Therefore, while rotating the intermittent rotating body 2 intermittently, analysis slides are sequentially supplied to the through holes at the peripheral edge of the intermittent rotating body 2, and the test sample, which has been previously separated using a centrifugal separator or the like in a separate process, is pipetted and analyzed. Drop onto the element surface of the slide. After the test sample is dispensed onto the element surface of all analysis slides, the light is sequentially measured by the photometry section. In this photometry, a photometric light beam from a light source is irradiated onto the measurement surface, the reflected light is calculated by a calculation device such as a microcomputer via a light receiving element, and the measured value is displayed on the display window of the main unit. There is.

However, in the case of the above-mentioned apparatus, the serum used as the test sample must be separated from whole blood in a separate step using a centrifugal separator or the like, and its handling is troublesome.

[Purpose of the invention]

This invention is intended to solve the above problems, and aims to provide a biochemical analyzer that can easily perform centrifugation of a test sample from an original sample and concentration measurement using the same device. .

[Structure of the invention]

In this invention, a plurality of through holes for fitting analysis slides are provided at the peripheral edge of the apparatus main body, an intermittent rotating body is provided that rotates intermittently at each installation angle of the through holes, and the intermittent rotating body is stopped. At times, there is a supply unit that fits an analysis slide into the through hole, a dispensing unit that drops a test sample onto the element surface of each fitted analysis slide, and a dispensing unit that drips a test sample onto the element surface of each fitted analysis slide. In a biochemical analyzer equipped with a photometric unit that measures and chemically analyzes changes in color density caused by the reaction, a high-speed rotating body that can centrifuge the test sample from the original sample is disposed above the device main body. It is configured as follows.

〔Example〕

Next, the present invention will be explained based on an embodiment shown in FIGS. 1 to 3.

Reference numeral 10 denotes a biochemical analyzer main body (hereinafter simply referred to as the main body), and an intermediate floor plate 11 is provided within the main body 10. A pedestal 12 is provided on the upper surface of the central portion of the intermediate floor plate 11, and a shaft rod 13 is vertically disposed on the upper surface of the pedestal 12.

Reference numeral 14 denotes an intermittent rotating body on which a plurality of through holes 16 for fitting the analysis slide 15 are arranged in the same manner on the periphery, and the intermittent rotating body 14 is rotatably fitted to the shaft rod 13. . An annular protrusion 18 having a gear 17 on the outer peripheral surface is provided on the lower surface of the intermittent rotating body 14, and the gear 17 has a drive gear 19 fixed to a spindle shaft 19a of a motor 19 attached to the intermediate floor plate 11.
b are engaged. This motor 19 can drive the intermittent rotating body 14 at predetermined angles (angles at which the through holes 16 are arranged), and is also capable of accurately stopping it at a predetermined position by fixed position stopping means (not shown). .

Reference numeral 20 denotes a high-speed rotating body, and the high-speed rotating body 20 is fixed to the upper end of a rotating shaft 21 provided so as to pass through the center of the shaft 13 that pivotally supports the intermittent rotating body 14 and penetrate the ceiling plate 10a of the main body 10. has been done. A pulley 22 that can rotate within the pedestal 12 is fixed to the lower end of the rotating shaft 21 . The pulley 22 is connected to a spindle 23 of a motor 23 attached to the intermediate floor plate 11.
Timing belt 2 is attached to pulley 23b fixed to a.
It is connected via 4. The motor 23 is, for example
It is now possible to obtain high speed rotation of 3000 rpm or more.

Reference numeral 25 denotes a test tube holding container that swingably hangs down from a plurality of holes 26, 26... equally spaced on the periphery of the top plate 20a of the high speed rotating body 20.
The lower end thereof can swing outward as shown by the two-dot chain line due to the centrifugal force generated when the high-speed rotating body 20 rotates.

Reference numeral 27 denotes an exterior cover of the high-speed rotating body 20. The exterior cover 27 is removably mounted on the top surface of the main body 1, and an opening/closing lid 28 is provided on the top surface. The opening of the opening/closing lid 28 exposes the opening of the test tube holding container 25 that hangs down into the hole 26 at the periphery of the high-speed rotary body 20, allowing for the attachment, replacement, and installation of the test tube 29 containing blood or the like. It is possible to take out the separated sample within the test tube using a pipette.

Reference numeral 30 denotes a supply section for fitting the analysis slide 15 into the through hole 16 of the intermittent rotating body 14, and the supply section 30 is provided at an appropriate position of the main body 10 corresponding to the stop position of the through hole 16. It is also possible to provide this supply unit 30 with a pusher device (not shown) that is linked to the stoppage of rotation of the intermittent rotating body 14 so that automatic supply can be performed.

Reference numeral 31 denotes a dispensing hole for dispensing separated serum to the analysis slide 15 that revolves together with the intermittent rotating body 14, and the dispensing hole 31 is located in the main body 1 and the outer shell 47, which will be described later, corresponding to the stop position of the analysis slide 15. It is provided in a penetrating manner on the upper surface. Note that the dispensing hole 31 provided in the outer shell 47 is closed after dispensing.

Reference numeral 32 denotes a photometry section, which, as shown in FIGS. 2 and 3, has a light source 33 such as a halogen lamp.
The light rays generated by the lens 34 and the filter 3
5 to form a photometric light beam of a desired wavelength, the photometric light beam is bent through a mirror 36, and guided through an optical fiber 37 to a photometric head 38 installed close to the measurement surface (back surface of the element) of the analysis slide 15. irradiated. This irradiation light is reflected from the measurement surface and transmitted to the light receiving element 40 through the optical fiber 39, and is calculated by a calculation device (not shown) such as a microcomputer, and the measured value is displayed on a display window provided at an appropriate location on the main body 1. (not shown). This measured value may be printed on a roll of recording paper (not shown) if necessary.

When the photometric head 38 is irradiated with the photometric light beam, it moves upward and presses against the measuring surface of the analysis slide 15 as shown in the third figure.

In addition, 41 is a transparent glass inclined at 45 degrees installed in the optical path of the photometric light beam in order to eliminate as much as possible errors in measurement values due to changes in the light intensity of the photometric light beam over time. A part of the reflected light is referred to a correction circuit (not shown) via the light receiving element 42 so that the measured value of the photometric light beam reflected from the measurement surface of the analysis slide 15 can be corrected to a correct value.

43 is a hot air generator, and the hot air generator 43 is attached to the center of the lower surface of the intermediate floor plate 11.
The illustrated hot air generator 43 has a heat dissipation plate 44 embedded with an electric heating element (not shown) mounted on an intermediate floor plate 11 in a mounting frame 4.
5, and a fan 46 is provided in the space between the intermediate floor plate 11 and the heat sink 44. The intermediate floor plate 11 and the heat sink 44 have a large number of through holes 11a, 44a.
As the fan 46 rotates, heated air is discharged into the upper chamber of the main body 10 through the holes in the intermediate floor plate 11 through the holes in the heat sink 44.

Reference numeral 47 denotes an outer skin covering the outer periphery of the main body 10, and the outer skin 47 includes a ventilation hole 48 provided in the main body 10 near the periphery of the intermittent rotating body 14, and a ventilation hole provided in the side wall of the lower chamber of the main body 10. 49, and the hot air entering the upper chamber from the hot air generator 43 is connected to the intermittent rotating body 14.
It passes around the analytical slide 15 fitted in the stepped through hole 16 on the peripheral edge of the slide, is guided to the outside of the main body 10 through the ventilation hole 48, descends inside the outer skin 47, and is allowed to flow back into the lower chamber through the ventilation hole 49. It's summery. The flow state of this hot air is shown by an arrow.

Next, the effect will be explained.

First, an opening/closing lid 28 is provided on the top surface of the exterior cover 27 of the main body 10, and a test tube holding container is swingably suspended in a plurality of holes 26, 26, . A test tube 29 containing an appropriate amount of whole blood is attached to the test tube 25, and the opening/closing lid is closed.
On the other hand, the analysis slides 15 are sequentially supplied from the supply section 30 to the stepped through holes 16 at the peripheral edge of the intermittent rotating body 14 in the main body 10 .

Next, when the motor 23 is started, the high-speed rotating body 20 is rotated at high speed by the motor 23.
Under the centrifugal force, the test tube 29 moves into its holding container 2.
5, the whole blood in the test tube 29 is separated into blood cells and serum due to the difference in specific gravity. After the time required for this separation has elapsed, the motor 23
The drive of the motor stops and is braked by a braking means (not shown). Thereafter, the opening/closing lid 28 is opened again, the separated serum in the test tube 29 is aspirated with a pipette (not shown), and the motor 19 is operated to stop rotating at a predetermined angle (the angle at which the stepped through hole 16 is arranged). The solution is dropped onto the element surface of the analysis slide 15 fitted into the stepped through hole 16 at the periphery of the intermittent rotating body 14 .

After the serum is dispensed onto the element surface of all the analysis slides 15, it reaches the installation position of the photometric head 38 of the photometric optical means 32, and is sequentially photometered. In this photometry, after the photometry head moves up and comes into close contact with the measurement surface of the analysis slide, the measurement surface is irradiated with a photometry beam from the light source 33, and the reflected light is processed by a calculation device such as a microcomputer via a light receiving element. The measured values are displayed on the display window of the main unit. At the same time, the information is recorded on a roll of recording paper as required.

The above operation is repeated after the measurement of all the elements on the analysis slides on the intermittent rotating body 14 is completed.

In the above case, the inside of the main body 10 is maintained at a predetermined temperature by the hot air generator 43, but the warm air generated by the hot air generator 43, ie, the rotation of the fan 46, is heated through the through hole of the heat sink 44. The air is released into the upper chamber of the main body 10 through the through holes in the intermediate floor plate 11, rises inside the main body 10, and is guided into the outer skin 47 of the main body 10 through the ventilation holes 48 near the periphery of the intermittent rotating body 14, It descends inside the outer shell 47 and flows back into the lower chamber through the ventilation holes 49, so that the temperature distribution inside the main body 10 becomes constant.

〔Effect of the invention〕

As described above, according to the present invention, a plurality of through holes for fitting an analysis slide are provided in the peripheral portion of the apparatus main body, and the intermittent slide is rotated intermittently at each installation angle of the through holes. A supply section that includes a rotating body and fits an analysis slide into its through hole when the intermittent rotating body is stopped; a dispensing section that drops the test sample onto the element surface of each fitted analysis slide; and a dispensing section that drops the test sample onto the element surface of each fitted analysis slide. In a biochemical analyzer equipped with a photometry unit that chemically analyzes the progress state or result of a reaction in an element due to dropping by measuring a change in color density due to the reaction,
The device is characterized by a high-speed rotating body that can centrifuge the test sample from the original sample at the top of the main body of the device, so whole blood can be used as it is as a test sample, and serum can be removed in a separate process as in the past. This method not only simplifies the handling of the test sample since there is no need to prepare a sample, but also has the excellent effect that the serum after separation can be used immediately as a test sample.

[Brief explanation of drawings]

The figures show one embodiment of the present invention, in which Fig. 1 is a sectional front view of the main body, Fig. 2 is a schematic diagram showing the photometric optical means, and Fig. 3 is a sectional view showing the relationship between the photometric head and the analysis slide. , FIG. 4 is a schematic cross-sectional view of a conventional example. 10...Main body, 14...Intermittent rotating body, 15...
Analysis slide, 16... Through hole, 20... High speed rotating body, 30... Supply section, 31... Dispensing section, 38...
Photometric head, 43... Warm air generator, 47... Outer shell.

Claims (1)

[Claims] 1. A plurality of through-holes for fitting an analysis slide are arranged in the periphery of the device body, and an intermittent rotating body is provided that rotates intermittently at each installation angle of the through-holes,
A supply unit that fits an analysis slide into the through hole when the intermittent rotating body is stopped, a dispensing unit that drops a test sample onto the element surface of each fitted analysis slide, and a reaction of the element due to the dropping of the test sample. In a biochemical analyzer equipped with a photometric unit that chemically analyzes the progress state or result of a reaction by measuring changes in color density caused by the reaction, a high-speed centrifugal unit is installed at the top of the device body to allow centrifugation of the test sample from the original sample. A biochemical analyzer characterized by having a rotating body.