JPH0333650A - Fine tube type uniform velocity electrophoretic apparatus - Google Patents

Abstract

PURPOSE:To control a system wherein a specimen is present in large quantities without using a large-scaled system such as a robot by earthing the terminal electrode provided to a terminal electrode cell to which a specimen introducing pipeline is connected directly. CONSTITUTION:An apparatus is constituted of a leading electrode cell 2 having a leading electrode 21, a terminal electrode cell 3 having a terminal electrode 32, the migration fine tube 4 connecting both electrode cells 2, 3, the detector 5 provided on the fine tube 4, the specimen introducing part 6 provided on the side of the electrode cell 3 from the detector 5 on the fine tube 4 and a high voltage constant current source 7 capable of applying voltage between the electrodes 21, 31. A specimen introducing pipeline 35 is connected to the electrode cell 3 through a peristaltic pump 33 and a stopper valve 34 and the electrode 31 is earthed. The specimen introducing pipe 6 has two discharge pipelines 61, 62 and a mode wherein the electrode cell 3 is allowed to communicate the one discharge pipeline to allow the migration fine tube 4 to communicate with the other discharge pipeline and a mode allowing the electrode cell 3 to communicate with the migration fine tube 4 are constituted so as to be capable of being changed over each other.

Description

[Detailed description of the invention] (b) Industrial application fields The present invention relates to a capillary isotachophoresis device.

More specifically, the present invention relates to a capillary isotachophoresis device suitable for managing systems in which a large amount of sample liquid exists, such as river water or wastewater.

(B) Conventional technology Conventionally, a capillary type isotachophoresis device consists of an electrode wave tank that stores a leading liquid, an electrode wave tank that stores a terminal group, and a capillary tube for electrophoresis that communicates between these electrode acid tanks. A device is known which is mainly composed of a high voltage constant current power supply device capable of applying a predetermined voltage between the electrode acid bath. A sample injection part is provided at the connection part of the electrophoresis thin tube with the internal electrode liquid tank.

The above-mentioned device is intended for analyzing a small amount of sample, and therefore, the sample is usually injected from the sample injection part to the interface between the electrophoresis capillary sliding liquid and the terminal group using a micro-nolino, etc. After that, it is configured to perform electrophoresis by applying a constant current.

(c) Problems to be Solved by the Invention However, with the above-mentioned conventional apparatus, even when used for the management of systems where a large amount of samples exist, such as the management of river water, wastewater, and bath management of bathtubs, it is difficult to apply high voltage. Therefore, it was necessary to draw a sample and inject it into the device each time a measurement was performed.

The present invention was made in view of the above situation, and aims to provide a capillary isotachophoresis device suitable for managing systems containing a large amount of samples without using a large-scale system such as a robot. be.

(d) Means for Solving the Problems According to the present invention, there is provided a leading electrode tank having a leading electrode and an electrode solution introduction path, a terminal electrode tank having a terminal electrode and an electrode solution introduction path, and these electrodes. A capillary for electrophoresis = 3 that communicates between the tanks, a detector provided near the leading electrode tank on the capillary for migration, and a sample provided closer to the terminal electrode tank than the detector setting position on the capillary for driving. A capillary type constant velocity electrolysis device comprising an introduction part and a high voltage constant current source capable of applying a predetermined voltage between the electrodes,
) A sample introduction flow path is connected to the terminal electrode tank via a liquid feeding means and a flow path opening/closing valve, (b) the terminal electrode tank is grounded, and (c) the sample introduction section is
It has two discharge channels, one mode in which the terminal electrode tank is connected to one discharge channel and the electrophoresis thin tube is connected to the other discharge channel, and the other mode is in which the terminal electrode bath is connected to the electrophoresis thin tube. A capillary isokinetic electrophoresis device is provided which includes a sample switching introduction valve configured to be switchable between modes.

For the apparatus of this invention, structures known in the art can be used, except that the terminal electrode tank is provided with a sample introduction channel, the terminal electrode side is grounded, and the electrophoresis thin tube is provided with a sample switching introduction valve. .

In the apparatus of this invention, the sample introduction flow path provided in the terminal electrode tank is connected to the electrode tank via the liquid feeding means and the flow path opening/closing valve in this order. As the liquid feeding means, for example, a peristaltic pump or the like is preferably used.

The sample switching introduction valve provided in the electrophoresis thin tube of this invention is as follows:
Two independent communication paths (A) that constitute the trap section (
B) and two discharge channels (C) and (D), the terminal electrode tank is connected to one of the discharge channels (C) via the communication channel (A), and the electrophoresis capillary is connected to the terminal electrode tank. A first mode connected to the other discharge flow path (D) via the passage (B);
Any structure may be used as long as it can be switched to the second mode in which the terminal electrode tank is connected to the electrophoresis capillary via the communication path (B). The sample is introduced into the sample switching introduction valve from the sample introduction channel through the terminal electrode tank.

In the apparatus of the present invention configured as described above, high voltage is applied so that the leading electrode has a positive potential when anions are to be analyzed, and the leading electrode has a negative potential when cations are to be analyzed. The polarity of the constant current source is set.

In addition, as a modification within the scope of the technical concept of the present invention, it is also possible to provide a sample introduction channel similar to the above in the leading electrode tank, and to ground the leading electrode side. In this case, the polarity of the high-voltage constant current source is reversed when the analyte is an anion or a cation, respectively.

Furthermore, in a configuration example in which a sample introduction channel is provided in each of the leading electrode tank and the terminal electrode tank, a switchable configuration is used in which the high voltage constant current source can be grounded to either the leading electrode side or the terminal electrode side. (+) -(0) or (-) depending on the analysis target
-(0) is configured to be used.

(E) Function According to the present invention, the terminal electrode side is grounded, and the sample introduction channel communicating with the terminal electrode tank has the same potential as the sample source on the ground directly connected.

Further, since the sample introduction section is composed of a valve that can temporarily hold the sample and switch it into the electrophoresis thin tube, the sample from the sample source can be analyzed directly and continuously.

The present invention will be described in detail below with reference to Examples, but the present invention is not limited thereby.

(F) Embodiment FIG. 1 is an explanatory diagram of the configuration of an example of a capillary type isotachophoresis apparatus of the present invention. In this figure, the device (1) includes a leading electrode (21) and a leading liquid introduction channel (22).
and a leading electrode tl! (2), a terminal electrode tank (3) having a terminal electrode (31) and a terminal liquid introduction path (32), a migration capillary (4) communicating between these electrode tanks, and an electrophoresis capillary (4) on the migration capillary. a potential gradient detector (5) provided near the leading electrode tank;
It mainly consists of a sample introduction part (6) provided on the terminal electrode tank side with respect to the detector setting position on the electrophoresis capillary, and a high voltage constant current source (7) capable of applying a predetermined voltage between the electrodes. has been done.

The terminal electrode side of the high voltage constant current source (7) of the device (1) is grounded (A).

1. The terminal electrode tank (3) has a peristaltic pump (33
) and a stop valve (34) in this order to connect to the electrode tank.

As shown in FIG. 2, the sample introduction section (6) includes two discharge pipes (61) (62) extending to the electrophoresis thin tube 5 (4), the terminal electrode tank communication channel (41), and the drain. )
The flow path switching valve is composed of a stator (63) to which are connected to each other, and a rotor (64) having two independent communication paths (A) and (B) that function as trap sections. The communication paths (A) and (B) above have the same capacity (
It is designed to trap approximately 5 μ0 sample limbs.

Note that the leading liquid introduction path (22) and the terminal liquid introduction path (32) are both provided with a liquid feeding pump and a stop valve (not shown).

Next, the operation of the above device (1) will be explained.

First, as shown in FIG.
1) and the discharge pipe (61) and a communication passage (A).
The rotor is set so that the electrophoresis thin tube (4) and the discharge conduit (62) are connected.

Next, the leading group is introduced into the leading liquid introduction channel (22
), the reducing liquid is passed through the leading electrode tank (2) - the thin tube for electrophoresis (4) - the continuous passageway (A) - the discharge pipe (62), and is discharged into the train.

On the other hand, after closing the terminal liquid inlet passage (32) with a stop valve (not shown), the stop valve (34) is opened and the peristaltic pump (33) is driven. ) to the sample introduction tube (
35), terminal electrode tank (3) - terminal electrode tank side communication path (41) → communication path (B) - discharge pipe (
61) and discharge it to the drain. By the above operations, the communicating path (A) is filled with the loading group, and the communicating path (B) is filled with the sample liquid.

Next, as shown in Fig. 3, the flow path switching valve of the sample switching introduction part (6) is connected so that the communication path (A) connects the terminal electrode tank side communication path (41) and the discharge pipe (61). And communication passage (
B) is set by rotating the rotor to connect the electrophoresis thin tube (4) and the discharge conduit (62).

In this state, the stop valve (34) is closed, and the liquid feeding pump (not shown) is driven to feed the terminal group from the terminal liquid introduction path (32) to the terminal electrode tank (3) - terminal electrode side communication path (41) continuous passage. (A) - Discharge pipe (
61) and discharged to the drain.

By the above operations, the communication path (B) is filled with the sample liquid, and the communication path (A) and the terminal electrode tank (3) are filled with the terminal liquid.

Furthermore, as shown in Fig. 4, the flow path switching valve of the sample switching introduction part (6) is connected so that the communication path (B) connects the terminal electrode tank side communication path (41) and the electrophoresis thin tube (4). In addition, the rotor is rotated to switch and set the communication path (A) to connect the discharge pipes (61) and (62). Thereby, the communication path (B) is inserted between the electrophoresis thin tube (4) and the terminal electrode side communication path (41). That is, the sample liquid filled in the communication path (B) is inserted into the interface between the terminal group and the loading liquid, and becomes ready for analysis.

In the above state, the polarity of the high voltage constant current source (7) whose terminal electrode (3() side is grounded) is set so that the leading electrode (2I) side is (+) when anions are to be analyzed. In addition, when cations are to be analyzed, the polarity of the power supply is set so that the leading electrode (2I) side is (-).

With the above configuration, a high voltage (several kV to lO several kV) is applied for electrophoresis, but since the terminal electrode (31) side is always grounded, the sample source (a) is also kept at OV and is safe.

(G) Effects of the Invention According to this invention, since the electrode on the side of the electrode tank directly connected to the sample introduction path is grounded, migration analysis can be performed by directly connecting the sample introduction path to a sample source such as a river, drainage, or bathtub. be able to. Therefore, a convenient continuous method for regularly monitoring these sample sources is available.
Unmanned systems can be provided.

[Brief explanation of drawings]

FIG. 1 is an explanatory diagram of the configuration of an example of the capillary isotachophoresis apparatus of the present invention, FIG. 2 is an explanatory diagram of the planar configuration of an example of the sample introduction section of the apparatus of FIG. FIG. 2 is a planar configuration explanatory diagram illustrating the flow path switching state of the sample introduction section, respectively. (2)... Leading electrode tank, (3)... Terminal electrode tank, (4)...
...Thin tube for electrophoresis, (5) ...Potential gradient detector, (6) ...Sample introduction section, (7) ...High voltage constant current source, (21) ...Reading Electrode, (2
2)... Leading liquid introduction path, (31)... Terminal electrode, (32)... Terminal liquid introduction path, (33)...
... Peristaltic pump, (34) ... Stop valve, (35) ... Sample introduction pipe, (41) ... Terminal electrode tank connection pipe, (61
), (62)...Discharge pipe, (63)...
...Stealth, (64) ...Rotor, (A
), (B)...Communication path.

Claims (1)

[Claims] 1. A leading electrode tank having a leading electrode and an electrode solution introduction path, a terminal electrode tank having a terminal electrode and an electrode solution introduction path, and a capillary for electrophoresis that communicates between these electrode tanks. A predetermined voltage is applied between a detector provided near the leading electrode tank on the electrophoresis capillary, a sample introduction part provided on the terminal electrode tank side from the detector setting position on the migration capillary, and the electrodes. A capillary isokinetic electrophoresis apparatus comprising: a high-pressure constant current source capable of applying a constant current; (b) the terminal electrode side is grounded; (c) the sample introduction section has two discharge channels, the terminal electrode tank is connected to one discharge channel, and the electrophoresis thin tube is connected to the other A capillary isokinetic electrophoresis device comprising a sample switching inlet valve configured to be switchable between a mode in which the terminal electrode tank communicates with the discharge flow path and a mode in which the terminal electrode tank communicates with the capillary tube for electrophoresis.