JPH0328759A - Wick for optical train - Google Patents
Wick for optical trainInfo
- Publication number
- JPH0328759A JPH0328759A JP16485989A JP16485989A JPH0328759A JP H0328759 A JPH0328759 A JP H0328759A JP 16485989 A JP16485989 A JP 16485989A JP 16485989 A JP16485989 A JP 16485989A JP H0328759 A JPH0328759 A JP H0328759A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- wick
- cup
- area
- optical train
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000003287 optical effect Effects 0.000 title abstract 3
- 238000004809 thin layer chromatography Methods 0.000 abstract description 13
- 230000035945 sensitivity Effects 0.000 abstract description 9
- 238000001514 detection method Methods 0.000 abstract description 6
- 238000004458 analytical method Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 239000012141 concentrate Substances 0.000 abstract description 2
- 238000004451 qualitative analysis Methods 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 239000000843 powder Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野コ
本発明は、ンンレイヤクロマトグラフィー（ＴｈＩｎ
Ｌａｙｅｒ Ｃｈｒｏｍａｔｏｇｒａｐｈｙ：以下、Ｔ
ＬＣと称す）と赤外線分光計とを組み合わせて定性分析
を行う場合に使用するオブチトレインに係り、特に、Ｔ
ＬＣプレートと試料カップとの間に介在するウィックの
形状に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention is directed to thin layer chromatography (ThIn
Layer Chromatography: Hereafter, T
In particular, T
This relates to the shape of the wick interposed between the LC plate and the sample cup.
［従来の技術コ
従来、ＴＬＣと赤外線分光計とを組み合わせて定性分析
を行うことが知られている。その概略を第２図、第３図
、第４図および第５図を参照して説明する。[Prior Art] Conventionally, it has been known to perform qualitative analysis by combining TLC and an infrared spectrometer. The outline thereof will be explained with reference to FIGS. 2, 3, 4, and 5.
まず、第２図に示すように、２．５ｃｍＸ４０ｃｍ程度
の大きさのＴＬＣプレート１１にサンプル１３を付着さ
せ、展開槽１０の中で適当な有機溶媒からなる展開溶媒
１２に浸す。これにより、サンプル１３中の各成分（以
下、これを試料と称す）は図中の矢印の方向に移動し、
１４、１５、１８で示すように各試料毎に分離される。First, as shown in FIG. 2, a sample 13 is attached to a TLC plate 11 having a size of about 2.5 cm x 40 cm, and immersed in a developing solvent 12 made of a suitable organic solvent in a developing tank 10. As a result, each component in the sample 13 (hereinafter referred to as the sample) moves in the direction of the arrow in the figure.
Each sample is separated as shown by 14, 15, and 18.
これが１回目の展開である。なお、ＴＬＣプレート１１
は、通常、ガラス基板にンリカゲルを塗布して形成され
る。This is the first development. In addition, TLC plate 11
is usually formed by coating a glass substrate with linica gel.
試料の展開が終了すると、ＴＬＣプレー｝１１は展開槽
１０から取り出され、第３図に示すようにオプチトレイ
ン１７にネジ１９により取り付けられ、固定される。オ
プチトレイン１７の上面には、直径が約１ ｍｍの開口
が約１．６１Ｉｍｌのピッチで複数個形成されている。When the development of the sample is completed, the TLC plate 11 is taken out from the development tank 10, and as shown in FIG. 3, it is attached and fixed to the Optitrain 17 with screws 19. A plurality of openings each having a diameter of approximately 1 mm are formed on the upper surface of the OptiTrain 17 at a pitch of approximately 1.61 Iml.
これが試料カップ１８である。This is the sample cup 18.
第３図のＡ−Ａにおける断面を第４図に示すが、試料カ
ップ１８の内部には、例えばＴＩガラス（Ｇ ｅ２ｅｓ
ｂ＋２ｓ ｅａｅ）等の赤外透過性粉体が充填されて
いる。また、試料カップ１８の底部には、その一端が赤
外透過性粉体に接するように、多孔質のステンレスから
なる円柱状のウィック２２が配置されている。ＴＬＣプ
レート１１の試料が展開された側はネジｌ９により当該
ウィック２２の側壁に接触するように固定されている。FIG. 4 shows a cross section taken along line A-A in FIG.
It is filled with infrared transparent powder such as b+2s eae). Further, a cylindrical wick 22 made of porous stainless steel is arranged at the bottom of the sample cup 18 so that one end thereof is in contact with the infrared transparent powder. The side of the TLC plate 11 on which the sample was developed is fixed by screws 19 so as to be in contact with the side wall of the wick 22.
さて、第３図に示す状態で２回目の展開を行う。Now, the second expansion is performed in the state shown in FIG.
即ち、ＴＬＣプレート１１をオプチトレイン１７に取り
付けた状態で、第５図に示すように、展開槽２０の中に
ＴＬＣプレート１１を横倒の状態で配置し、適当な展開
溶媒２１に浸すことによって試料を１回目の展開の方向
と直角方向に展開するのである。このことにより、ＴＬ
Ｃプレート１１に展開された各試料は展開溶媒と共に第
５図の矢印の方向に移動し、最終的には、第４図のウィ
ック２２を通って試料カップ１８に充増された粉体に浸
透する。なお、試料の展開速度は展開溶媒２■を適宜選
択することで制御することができる。That is, with the TLC plate 11 attached to the Optitrain 17, as shown in FIG. The sample is developed in a direction perpendicular to the direction of the first development. By this, T.L.
Each sample developed on the C plate 11 moves in the direction of the arrow in FIG. 5 together with the developing solvent, and finally penetrates into the powder filled in the sample cup 18 through the wick 22 in FIG. do. Incidentally, the developing speed of the sample can be controlled by appropriately selecting the developing solvent 2.
２回目の展開が終了した後、試料カップ１８の表面に乾
燥空気あるいは窒素ガス等を吹き付けて展開溶媒を除去
し、試料だけを残した状態で各試料カップ１８に順次赤
外線を照射し、その反射光を検出、分析することで各試
料の定性分析を行うことができる。After the second development is completed, dry air or nitrogen gas is blown onto the surface of the sample cup 18 to remove the developing solvent, and each sample cup 18 is sequentially irradiated with infrared rays, leaving only the sample, and its reflection is measured. Qualitative analysis of each sample can be performed by detecting and analyzing light.
［発明が解決しようとする課題コ
以上のようにして試料の定性分析を行うことができるの
であるが、微量な試料に対しては感度よく分析できない
という問題があり、実際、上述した方法においては、試
料の検出限界は１００〜２００ｎｇ程度である。[Problems to be Solved by the Invention] Qualitative analysis of a sample can be performed in the above manner, but there is a problem that it is not possible to analyze a minute amount of sample with high sensitivity. , the detection limit of the sample is about 100 to 200 ng.
本発明は、上記の課題を解決するものであって、感度よ
く定性分析を行うことができるオプチトレイン用ウィッ
クを提供することを目的とするものである。The present invention solves the above-mentioned problems, and aims to provide a wick for OptiTrain that allows qualitative analysis to be performed with high sensitivity.
［課題を解決するための手段］
」二記の目的を達成するために、本発明のオプチトレイ
ン用ウィックは、オプチトレイン内部に配置され、試料
が展開されたプレートと試料力・ノブとの間に介在する
オプチトレイン用ウィックにおいて、当該ウィソクの試
料カップに当接する先端部の面積が他端部の面積より小
さくなされていることを特徴とする。[Means for Solving the Problems] In order to achieve the above two objects, the wick for OptiTrain of the present invention is arranged inside the OptiTrain, and is arranged between the plate on which the sample is developed and the sample force/knob. A wick for OptiTrain interposed therein is characterized in that the area of the tip of the wick that comes into contact with the sample cup of the wick is smaller than the area of the other end.
［作用および発明の効果コ
本発明は、ウイックの試料力・ンプに当接する側の面積
が小さくなされているので、試料を狭い範囲に濃縮する
ことができ、従って試料力・ンプ内の試料の密度を上げ
ることができるので、検出感度を向上させることができ
る。[Operation and Effects of the Invention] In the present invention, the area of the side of the wick that comes into contact with the sample force/pump is made small, so the sample can be concentrated in a narrow range. Since the density can be increased, detection sensitivity can be improved.
［実施例］ 以下、図面を参照しつつ実施例を説明する。[Example] Examples will be described below with reference to the drawings.
第１図は、本発明に係るオプチトレイン用ウイックの１
実施例の構成を示す断面図であり、図中、１はウィック
、２はオプチトレイン、３は試料カップを示す。FIG. 1 shows one of the wicks for OptiTrain according to the present invention.
1 is a cross-sectional view showing the configuration of an example, in which 1 indicates a wick, 2 indicates an optitrain, and 3 indicates a sample cup.
さて、従来例の定性分析方法において、検出感度がよく
ない原因を考察してみると、試料力・ンプ１８の面積が
大きいことが最犬の原因であることが判明した。つまり
、各試料はほぼ均一に試料カップ１８の表面に広がるか
ら、試料が微量の場合は試料の密度、即ち単位面積当り
の試料量が非常に少なくなり、その結果検出感度が悪い
ものになってしまうのである。Now, when we considered the reason why the detection sensitivity was not good in the conventional qualitative analysis method, it was found that the main reason was that the sample force and the area of the pump 18 were large. In other words, since each sample spreads almost uniformly on the surface of the sample cup 18, when the sample is in a small amount, the density of the sample, that is, the amount of sample per unit area, becomes very small, resulting in poor detection sensitivity. It's put away.
そこで、本発明においては、第１図に示すように、ウィ
ソク１の試料カップ３側の先端部の形状を円鉛．状とし
た。Therefore, in the present invention, as shown in FIG. 1, the tip of the sample cup 3 side of the Wisok 1 is shaped like a circular lead. It was made into a state.
これによって、試料カップ３の面積を、第４図に示す従
来の試料カップ１８に比して小さくすることができ、従
って、第４図に示すウィック２２に比較して試料の密度
を上げることができる。As a result, the area of the sample cup 3 can be made smaller compared to the conventional sample cup 18 shown in FIG. 4, and therefore the density of the sample can be increased compared to the wick 22 shown in FIG. can.
つまり、本発明は、試料を狭い範囲に濃縮するものであ
るから、試料が微量な場合にも感度よく分析を行うこと
ができるのである。In other words, since the present invention concentrates the sample in a narrow range, it is possible to perform analysis with high sensitivity even when the sample is in a trace amount.
以上、本発明の１実施例を説明したが、本発明は上記実
施例に限定されるものではなく、種々の変形が可能であ
る。即ち、上記実施例ではウイ・ンク１の先端部を円錐
状としたが、これは発明の木質ではなく、要するに、試
料力・ソプに当接する先端部の面積が他端部の面積より
小さくなる形状ならどのようなものでもよいことは当業
者に明らかである。Although one embodiment of the present invention has been described above, the present invention is not limited to the above embodiment, and various modifications are possible. That is, in the above embodiment, the tip of wink 1 is conical, but this is not the wood of the invention, and in short, the area of the tip that comes into contact with the sample force is smaller than the area of the other end. It will be clear to those skilled in the art that any shape may be used.
第１図は本発明の１実施例の構成を示す断面図、第２図
、第３図、第４図、第５図はＴＬＣと赤外分光計を組み
合わせた定性分析法を説明するための図であり、第２図
は１回目の展開を説明する図、第３図はＴＬＣプレート
をオプチトレインに固定した状態を示す図、第４図は第
３図のＡ’−Ａにおける断面図、第５図は２回目の展開
を説明する図である。
１・・・ウィック、２・・・オプチトレイン、３・・・
試料カップ。
出 願 人 日本電子株式会社FIG. 1 is a sectional view showing the configuration of one embodiment of the present invention, and FIGS. 2, 3, 4, and 5 are diagrams for explaining a qualitative analysis method combining TLC and an infrared spectrometer. FIG. 2 is a diagram explaining the first deployment, FIG. 3 is a diagram showing a state in which the TLC plate is fixed to Optitrain, and FIG. 4 is a cross-sectional view taken along A'-A in FIG. 3. FIG. 5 is a diagram illustrating the second development. 1...Wick, 2...Optitrain, 3...
sample cup. Applicant: JEOL Ltd.
Claims (1)
たプレートと試料カップとの間に介在するオプチトレイ
ン用ウィックにおいて、当該ウィックの試料カップに当
接する先端部の面積が他端部の面積より小さくなされて
いることを特徴とするオプチトレイン用ウィック。(1) In the OptiTrain wick that is placed inside the OptiTrain and interposed between the plate on which the sample is spread and the sample cup, the area of the tip of the wick that comes into contact with the sample cup is larger than the area of the other end. A wick for OptiTrain that is characterized by its small size.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16485989A JPH0328759A (en) | 1989-06-26 | 1989-06-26 | Wick for optical train |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16485989A JPH0328759A (en) | 1989-06-26 | 1989-06-26 | Wick for optical train |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0328759A true JPH0328759A (en) | 1991-02-06 |
Family
ID=15801275
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16485989A Pending JPH0328759A (en) | 1989-06-26 | 1989-06-26 | Wick for optical train |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0328759A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8491148B2 (en) | 2007-10-27 | 2013-07-23 | Osram Sylvania Inc. | Chambered waterproof lamp assembly having a transparent cover switch activator |
-
1989
- 1989-06-26 JP JP16485989A patent/JPH0328759A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8491148B2 (en) | 2007-10-27 | 2013-07-23 | Osram Sylvania Inc. | Chambered waterproof lamp assembly having a transparent cover switch activator |
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