JPH0322930A - Formation and proliferation of crown of plant of genus asparagus and proliferation of seedling - Google Patents
Formation and proliferation of crown of plant of genus asparagus and proliferation of seedlingInfo
- Publication number
- JPH0322930A JPH0322930A JP1154607A JP15460789A JPH0322930A JP H0322930 A JPH0322930 A JP H0322930A JP 1154607 A JP1154607 A JP 1154607A JP 15460789 A JP15460789 A JP 15460789A JP H0322930 A JPH0322930 A JP H0322930A
- Authority
- JP
- Japan
- Prior art keywords
- crown
- plant
- asparagus
- plants
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- 235000005340 Asparagus officinalis Nutrition 0.000 title claims abstract description 31
- 230000035755 proliferation Effects 0.000 title abstract description 7
- 230000015572 biosynthetic process Effects 0.000 title description 6
- 244000003416 Asparagus officinalis Species 0.000 title description 2
- 241000234427 Asparagus Species 0.000 claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 33
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 206010020649 Hyperkeratosis Diseases 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 12
- 238000012258 culturing Methods 0.000 claims abstract description 10
- 230000001902 propagating effect Effects 0.000 claims description 8
- 230000012010 growth Effects 0.000 claims description 2
- 238000005273 aeration Methods 0.000 abstract description 8
- 230000002062 proliferating effect Effects 0.000 abstract 1
- 230000008719 thickening Effects 0.000 abstract 1
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- 239000011684 sodium molybdate Substances 0.000 description 1
- 235000015393 sodium molybdate Nutrition 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 238000004017 vitrification Methods 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明はアスパラガス属植物のクラウン形或及び増殖方
法、並びに、植物体及び種苗増殖方法に関する.
〔従来の技術〕
アスパラガス属植物の増殖は、従来、播種もしくは株分
けによって行われてきた。アスパラガス属植物は、雌雄
異株であり、雄株の方が品質や栽培管理の点で良いとさ
れている。さらに、遺伝的に変異の大きな作物であるた
め播種による増殖は、雌株の混入を招き、又、株毎の収
量及び品質の差異を招き、栽培上著しい問題となってい
る。また、株分けによる増殖は多くの人手と長い年月を
必要とし、効率が非常に悪く、ウイルス病などが伝染し
ていく危険性も非常に高い。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a crown shape and a method for propagating plants of the genus Asparagus, and a method for propagating plants and seedlings. [Prior Art] Plants of the genus Asparagus have conventionally been propagated by seeding or division. Plants of the genus Asparagus are dioecious, and male plants are considered to be better in terms of quality and cultivation management. Furthermore, since it is a crop with large genetic variations, propagation by sowing leads to the contamination of female plants and causes differences in yield and quality among plants, which poses a significant problem in cultivation. In addition, propagation by division requires a lot of manpower and a long period of time, is extremely inefficient, and has a high risk of transmitting viral diseases.
以上のような問題点を改善する目的で、近年、組織培養
による優良株クローンの大量増殖が注目されている.通
常、アスパラガスの大量増殖は、組織片を培養し、多量
の苗条(shoot)を増殖させた後、各々を発根培地
に移植し、不定根の分化を経て幼苗となす。しかし、発
根率が一般的に低いために、増殖効率が低いという問題
点がある。また、組織片を培養し、カルスを形成させた
のち、カルスより生じる不定胚を用いて大量増殖の手法
とするための試みがなされている(例えば、昭和61年
度園芸学会秋季大会研究発表要旨P210〜211、昭
和61.ll.23〜25、於琉球大学、昭和62年度
園芸学会研究発表要旨P254〜255、昭和62.1
0.7〜9、於九州大学)。不定胚を生長させることに
より、発根率が低いという問題は解決するものの、単離
した不定胚はほとんどが培養の途中でカルス化あるいは
奇形化を引き起こすことが問題となっている。In order to improve the above-mentioned problems, mass propagation of superior strain clones using tissue culture has recently attracted attention. Normally, for mass propagation of asparagus, tissue pieces are cultured, a large number of shoots are propagated, and then each is transplanted to a rooting medium, and seedlings are formed through differentiation of adventitious roots. However, since the rooting rate is generally low, there is a problem that the propagation efficiency is low. In addition, attempts have been made to culture tissue pieces to form callus, and then use somatic embryos produced from the callus to mass multiply (for example, 1985 Horticultural Society Autumn Conference Research Presentation Abstracts, page 210). ~211, 1986.ll.23-25, University of the Ryukyus, 1988 Horticultural Society Research Presentation Abstracts P254-255, 1986.1
0.7-9, Kyushu University). Although growing somatic embryos solves the problem of low rooting rate, the problem is that most isolated somatic embryos develop callus or malformation during culture.
本発明者らは従来のアスパラガス属植物の組織培養方法
には前記した問題点のあることを認知した上で、従来法
とは異なる新規な方法によって組織培養してアスパラガ
ス属植物の種苗を効率よく増殖する方法について検討し
た.
〔課題を解決するための手段〕
その結果、本発明者等はアスパラガス属植物を組織培養
して増殖するに際し、支持材に含浸した液体培地を用い
通気条件下で組織培養してクラウンを形或させ植物体と
することにより上記従来技術の問題点を良好に解消し得
ることを見出し、この新知見に基づいてさらに研究を重
ねることにより本発明を完戒するに至ったものである。The present inventors recognized that the conventional tissue culture method for plants of the genus Asparagus has the above-mentioned problems, and cultivated the seeds and seedlings of plants of the genus Asparagus by culturing tissues using a new method different from the conventional method. We investigated methods for efficient propagation. [Means for Solving the Problems] As a result, the present inventors developed a technique for tissue culture and propagation of Asparagus plants by culturing the tissue under aerated conditions using a liquid medium impregnated with a supporting material to form a crown. It was discovered that the problems of the prior art described above could be satisfactorily solved by using a plant body, and by conducting further research based on this new knowledge, the present invention was completed.
したかっ”で、本発明の方法によれば、
■ アスパラガス属植物の組織又はカルスを支持材に含
浸した液体培地を用い通気条件下で培養してクラウンを
形成させることを特徴とするアスパラガス属植物のクラ
ウン形或方法、■ ■の方法でカルスを培養して形成さ
せたクラウンを切断して得た切片をさらに支持材に含浸
した液体培地を用い通気条件下で培養してクラウンを肥
大させ、さらに必要に応じて同様のクラウン切断→切片
培養→クラウン肥大の増殖プロセスを繰り返すことを特
徴とするアスパラガス属植物のクラウン増殖方法、
■ ■又は■の方法で得られたクラウンを支持材に含浸
した液体培地を用い通気条件下で培養して植物体を生育
させることを特徴とするアスパラガス属植物の植物体の
増殖方法、
■ ■の方法で得られた植物体を馴化せしめて種苗とす
ることを特徴とするアスパラガス属植物の種苗増殖方法
、
が提供される。According to the method of the present invention, (1) Asparagus is cultured under aerated conditions using a liquid medium in which a supporting material is impregnated with tissue or callus of a plant of the genus Asparagus to form a crown. The crown shape of plants of the genus is grown by culturing the callus using the method described in (1) and cutting the crown. A method for propagating crowns of plants of the Asparagus genus, characterized by repeating the same propagation process of crown cutting → section culture → crown enlargement as necessary; A method for propagating plants of the genus Asparagus, characterized by growing the plants by culturing them under aerated conditions using a liquid medium impregnated with Provided is a method for propagating seeds and seedlings of plants of the genus Asparagus, characterized by:
本発明では、アスパラガス属に属する植物であればすべ
て使用できる。該植物として具体的には食用アスパラガ
スであるAsparagus officinalis
L.var.altilis L.を例示でき、本発明
ではこれを用いるのが好ましい。In the present invention, any plant belonging to the genus Asparagus can be used. Specifically, the plant is Asparagus officinalis, which is an edible asparagus.
L. var. altilis L. For example, it is preferable to use this in the present invention.
本発明ではアスパラガス属植物の組織又はカルス(ca
Hus)が組織培養されてクラウン(crown)が形
成される.この場合のMi織培養に用いられる組織とし
ては胚、茎頂、茎、擬葉、地下茎等のIJlwiを例示
できる。カルスについては、本発明では該組織を例えば
公知の培養方法(例えば、園芸学会雑誌 第40巻 第
4号 11頁〜17頁, 1971年)によって培養し
て得られるカルスを用いることができる。In the present invention, tissues or callus (ca) of plants of the genus Asparagus are used.
Hus) is tissue cultured to form a crown. In this case, IJlwi such as embryo, shoot tip, stem, pseudoleaf, underground stem, etc. can be exemplified as the tissue used for Mi tissue culture. Regarding callus, in the present invention, callus obtained by culturing the tissue by a known culture method (for example, Horticultural Society Journal, Vol. 40, No. 4, pp. 11-17, 1971) can be used.
本発明のクラウン形或において使用される培地は、無機
成分及び炭素源を必須戒分とし、これに植物ホルモン類
、ビタミン類を添加し、更に必要に応じてアミノ酸類を
添加した培地である。該培地の無I!!戒分としては、
窒素、リン、カリウム、ナトリウム、カルシウム、マグ
ネシウム、イオウ、鉄、マンガン、亜鉛、ホウ素、モリ
ブデン、塩素、ヨウ素、コバルト等の元素を含む無機塩
をあげることができ、具体的には、硝酸カリウム、硝酸
ナトリウム、硝酸アンモニウム、塩化カリウム、塩化カ
ルシウム、リン酸1水素カリウム、リン酸2水素ナトリ
ウム、硫酸マグネシウム、塩化マグネシウム、硫酸ナ}
IJウム、硫酸第1鉄、硫酸第2鉄、硫酸マンガン、
硫酸銅、モリブデン酸ナトリウム、三酸化モリブデン、
ヨウ化カリウム、硫酸亜鉛、ホウ酸、塩化コバルト等の
化合物を例示できる。The medium used in the crown type of the present invention is a medium containing inorganic components and a carbon source as essential ingredients, to which are added plant hormones and vitamins, and further added with amino acids as necessary. No I of the medium! ! As a precept,
Examples include inorganic salts containing elements such as nitrogen, phosphorus, potassium, sodium, calcium, magnesium, sulfur, iron, manganese, zinc, boron, molybdenum, chlorine, iodine, and cobalt. Specifically, potassium nitrate, nitric acid Sodium, ammonium nitrate, potassium chloride, calcium chloride, potassium monohydrogen phosphate, sodium dihydrogen phosphate, magnesium sulfate, magnesium chloride, sodium sulfate}
IJum, ferrous sulfate, ferric sulfate, manganese sulfate,
copper sulfate, sodium molybdate, molybdenum trioxide,
Examples include compounds such as potassium iodide, zinc sulfate, boric acid, and cobalt chloride.
該培地の炭素源としては、庶糖やグルコースなどの炭水
化物、その誘導体、脂肪酸などの有機酸及びエタノール
等の1級アルコールなどを例示できる。Examples of carbon sources for the medium include carbohydrates such as sucrose and glucose, derivatives thereof, organic acids such as fatty acids, and primary alcohols such as ethanol.
該培地の植物ホルモンとしては、例えば、ナフタレン酢
酸(NA^)、インドール酢酸(14A)、pクロロフ
ェノキシ酢酸、2,4−ジクロロフェノキシ酢酸(2.
4−[))、インドール酪酸(IB八)、及びこれら
の誘導体等のオーキシン類及びペンジルアデニン(BA
)、カイネチン、ゼアチン等のサイトカイニン類を例示
できる。Examples of the plant hormones in the medium include naphthaleneacetic acid (NA^), indoleacetic acid (14A), p-chlorophenoxyacetic acid, and 2,4-dichlorophenoxyacetic acid (2.
Auxins such as 4-[)), indolebutyric acid (IB8), and their derivatives, and penzyladenine (BA
), kinetin, zeatin, and other cytokinins.
該培地のビタ旦ン類としては、ビオチン、チアミン(ビ
タミンB,)、ビリドキシン(ビタミンB6)、ピリド
キサール、ビリドキサミン、パントテン酸カルシウム、
アスコルビン酸(ビタミンC)、イノシトール、ニコチ
ン酸、ニコチン酸アミド及びリポフラビン(ビタミンB
6)などを例示できる。The vitamins in the medium include biotin, thiamine (vitamin B), pyridoxin (vitamin B6), pyridoxal, pyridoxamine, calcium pantothenate,
Ascorbic acid (vitamin C), inositol, nicotinic acid, nicotinamide and lipoflavin (vitamin B
6) etc. can be exemplified.
該培地のアミノ酸類としては、例えばグリシン、アラニ
ン、グルタミン、システィン、フエニルアラニン及びリ
ジンなどを例示できる。Examples of amino acids in the medium include glycine, alanine, glutamine, cysteine, phenylalanine, and lysine.
本発明の前記培地は、通常は、前記無機成分を約0.
1μHないし約100lIM、前記植物ホルモン類を約
0.01mg/j!ないし約150mg/ 1及び前記
アミノ酸類を0ないし約1000■/1含ませて使用さ
れることが望ましい.
本発明に係わる組織培養に用いられる前記培地として具
体的には、従来から知られている植物の組織培養に用い
られている培地、例えば、ムラシゲ・スクーグ(’62
) [Murashige & Skooglの培地、
リンスマイヤー・スクーグ(RM−1965) [Li
nsa+aier& Skoog]の培地、ホワイト
(’63) [White]の培地、ガンボルグ[Ga
mborg]のB−5培地、三井の門9培地、ニッチ・
ニッチの培地[Nitch & Nitch]等に前記
した炭素源及び植物ホルモンを添加し、更に必要に応じ
て前記したビタ藁ン類、ア藁ノ酸類を添加して調整され
た培地を例示できるが本発明ではこの中でも特にニッチ
・エッチ、リンスマイヤー・スクーグ、ムラシゲ・スク
ーズの培地を用いて調整される培地が好ましい。上記し
た従来公知の培地のMi戒に関しては、例えば、竹内、
中嶋、古谷著の「新植物組織培養, p3B6〜p39
1、朝倉書店、1979年に記載されている。本発明の
クラウン形或方法において使用できる前記培地は、液体
培地であり、ポリエステル、ポリオレフイン、ポリビニ
ルクロライド、ポリビニリデン、ポリアクリルニトリル
アセテートアルコールなどのボリマー類、ロックウール
、バーミキュライト、バーライトなどの人工土壌、プラ
スチック網、金網等の支持体に含浸して用いる。The medium of the present invention usually contains about 0.0% of the inorganic component.
1 μH to about 100 lIM, about 0.01 mg/j of the plant hormones! Preferably, the amino acids are used in an amount of 0 to about 150 mg/1 and 0 to about 1000 mg/1. Specifically, the medium used for tissue culture according to the present invention may be a conventionally known culture medium used for plant tissue culture, such as Murashige Skoog ('62
) [Murashige &Skoogl's medium,
Linsmeyer Skoog (RM-1965) [Li
nsa+aier&Skoog] medium, white
('63) [White] medium, Gamborg [Ga
mborg] B-5 medium, Mitsui Mon 9 medium, niche
An example is a medium prepared by adding the above-mentioned carbon source and plant hormone to a Nitch medium [Nitch & Nitch], and further adding the above-mentioned bitter straws and straw acids as necessary, but this In the present invention, among these, media prepared using Niche-Hetch, Linsmeyer-Skoog, and Murashige-Skoos are particularly preferred. Regarding the Mi precepts of the conventionally known culture medium mentioned above, for example, Takeuchi,
“New Plant Tissue Culture,” by Nakajima and Furuya, p3B6-p39
1, Asakura Shoten, 1979. The medium that can be used in the crown-shaped method of the present invention is a liquid medium, and includes polymers such as polyester, polyolefin, polyvinyl chloride, polyvinylidene, and polyacrylonitrile acetate alcohol, and artificial soil such as rock wool, vermiculite, and barlite. It is used by impregnating a support such as a plastic mesh or wire mesh.
本発明では前記液体培地を用い通気条件下でアスパラガ
ス属植物の組織又はカルスを組織培養してクラウンを形
成させるものである。In the present invention, tissue or callus of a plant of the genus Asparagus is tissue cultured using the liquid medium under aerated conditions to form a crown.
この場合の本発明で言うところのクラウンとは全植物体
中の地下茎に相当し形態的には短縮茎にあたる貯蔵組織
であり、組織又はカルスを組織培養して得られる、塊状
ないしは冠状の形状をした細胞の塊であって幼芽あるい
は幼芽の基になる幼芽原基を含んでいるものをさす。In this case, the crown as used in the present invention is a storage tissue that corresponds to the underground stem in the whole plant and is morphologically a shortened stem, and has a lump-like or crown-like shape obtained by tissue culture of tissue or callus. A cluster of cells that contains buds or bud primordia that become the basis of buds.
通気の方法としては、エアーポンプ、ガスボンベ、ファ
ンなどを用いた強制通気とフィルターをつけた培養器に
風をあてる、温度変化による空気の膨張、収縮を利用す
るなどの自然換気などがあげられる。使用する気体とし
ては酸素、二酸化炭素、窒素、空気などのうち2種類以
上の気体を混合した気体を用いることができる。本発明
では特に、酸素が5〜20vo1/χ、二酸化炭素が4
00〜1000ppmを含む混合気体が望ましい。通気
速度は、培養容器の形状や大きさにより異なるが、培養
容器内気相部分の換気回数が1〜20回/hが望ましい
。Aeration methods include forced ventilation using air pumps, gas cylinders, fans, etc., blowing air through culture vessels equipped with filters, and natural ventilation using air expansion and contraction due to temperature changes. As the gas to be used, a mixture of two or more types of gases such as oxygen, carbon dioxide, nitrogen, and air can be used. In particular, in the present invention, oxygen is 5 to 20 vol/χ, carbon dioxide is 4
A mixed gas containing 00 to 1000 ppm is desirable. Although the ventilation rate varies depending on the shape and size of the culture container, it is preferable that the gas phase portion within the culture container be ventilated 1 to 20 times/h.
クラウン形成に使用する培養装置としては、液体培地を
含浸させるための支持材を有し、気相部の通気を行いう
るようにしたものであれば、何ら限定されない。具体的
には、第2図に示すような装置が使用できる。The culture device used for crown formation is not limited in any way as long as it has a support material for impregnating it with a liquid medium and can ventilate the gas phase. Specifically, an apparatus as shown in FIG. 2 can be used.
第1図は、本発明の方法によるアスパラガス属植物のク
ラウン形或、増殖、植物体の増殖等のプロセスを示す図
である.図の上部には、苗条の茎を切断した得られた切
片を組織培養してクラウンが形或される経路が示されて
おり、図の下部の左側には、前記クラウンからクラウン
を増殖する増殖サイクルが示され、図の下部の右側には
、このようにして増殖されたクラウンから発根、再生さ
せた植物体が示されている。FIG. 1 is a diagram showing the process of crown formation, proliferation, and plant growth of Asparagus plants according to the method of the present invention. The upper part of the figure shows the path through which the crown is formed by tissue culturing the cut sections obtained from the shoot stem, and the lower left part of the figure shows the route by which the crown is grown from said crown. The cycle is shown, and the bottom right side of the figure shows a plant that has been rooted and regenerated from the crown that has been propagated in this way.
本発明においてクラウンを形成させるための培地として
は前述のような培地を使用できるが、特にシーJ糖を初
朋糖濃度として3%以上含有する培地を用いた場合にク
ラウン形或率を高めることができる。In the present invention, the above-mentioned medium can be used as a medium for forming a crown, but in particular, when using a medium containing 3% or more of C.J. I can do it.
本発明では、クラウン形成の際通気条件を採用すること
により、維管束系の発達した良質のクラウンの形成でき
、かつ形或速度を向上させることができる。得られたク
ラウンは、発根しやすく、またビトリフィケーションを
避けることができる。In the present invention, by employing aeration conditions during crown formation, a high-quality crown with a developed vascular system can be formed, and the shape and speed can be improved. The resulting crowns are easy to root and avoid vitrification.
本発明では、上記のようにして形成させたクラウンを切
断し、好ましくは少なくとも1個の芽を含むように切断
し、得られた切片をさらに支持材に含浸させた液体培地
を用い通気条件下で培養しすることによりクラウンを肥
大威長させることができる.
上記クラウンの肥大、増殖を行うための液体培地はクラ
ウン形成に使用するのと同様のものが使用でき、特にシ
amを3%(初期濃度)以上含有する培地を用いた場合
に、クラウンの増殖率を向上させうる。通気条件は前述
と同様でよい。In the present invention, the crown formed as described above is cut, preferably to include at least one bud, and the obtained cut pieces are further used in a liquid medium impregnated with a supporting material under aerated conditions. The crown can be enlarged and elongated by culturing it. The same liquid medium as that used for crown formation can be used for the above-mentioned crown enlargement and proliferation, and especially when using a medium containing 3% (initial concentration) or more of siam, crown proliferation rate can be improved. Ventilation conditions may be the same as described above.
本発明では、クラウンの肥大において通気条件を採用す
ることにより、良質のクラウンを得る、即ち、発根しや
すいクラウンを得て、ビトリフィケーシジン防止するこ
とができる。In the present invention, by adopting aeration conditions during crown enlargement, a high-quality crown can be obtained, that is, a crown that is easy to root, and vitrificesidin can be prevented.
さらに必要に応じて、同様のクラウン切断一切片組織培
養一クラウン肥大の増殖プロセスを繰り返すことにより
クラウンの増殖を行うことができる。Furthermore, if necessary, crown propagation can be performed by repeating the same crown cut section tissue culture-crown enlargement propagation process.
次いで上記のようにして増殖されたクラウンの中から適
宜量のクラウンを抜き取って発根培地で培養して発根さ
せ、前記芽を適宜大きさの植物体に生育させる。この植
物体への生育工程も、前述のクラウン形成に用いたと同
様の液体通気培養によれば、貯蔵根の発根率及び発根速
度を高め、良質の苗をうることかできる。Next, an appropriate amount of crowns are extracted from the crowns propagated as described above and cultured in a rooting medium to root, and the buds are grown into plants of an appropriate size. In this process of growing the plant, liquid aeration culture similar to that used for the crown formation described above can be used to increase the rooting rate and rooting speed of storage roots and to obtain high-quality seedlings.
発根は培地中に抗ジベレリン剤を添加した場合に特に促
進される.抗ジベレリン剤としては具体的にはアンシ壽
ドールが挙げられる.
さらに本発明では、必ずしも馴化工程は必要でないが、
必要に応じて上記植物体を馴化することによって種苗と
することができ、以上の方法を繰り返すことによりアス
パラガス属植物の種苗を大量に増殖させることができる
。Rooting is particularly promoted when an anti-gibberellin agent is added to the medium. A specific example of an anti-gibberellin agent is Ansijudol. Furthermore, in the present invention, although an acclimatization step is not necessarily required,
If necessary, the above-mentioned plants can be acclimatized to produce seedlings, and by repeating the above method, seeds and seedlings of Asparagus plants can be propagated in large quantities.
以下、本発明の方法を実施例によって具体的に示す。Hereinafter, the method of the present invention will be specifically illustrated by examples.
実施例1
アスパラガスの品種゛北海100”の無菌系で維持して
いるシュートを切断して、長さ7〜10+a+aに調整
した節を材料とした.培地は、無機要素をMS培地、有
機要素をニッチ・ニッチを基本とし、NH4NO3を1
/2倍に、CaCl.を2倍とし、グルタミン104M
を添加したものを基本培地(以後、ASP培地と略記)
とした。Example 1 The shoots of the asparagus variety "Hokkai 100" maintained in a sterile system were cut and the nodes adjusted to a length of 7 to 10+a+a were used as material.The medium was an MS medium containing inorganic elements and an organic element. Based on niche/niche, NH4NO3 is 1
/2 times CaCl. 2 times, glutamine 104M
Basic medium (hereinafter abbreviated as ASP medium)
And so.
培地は、ASP培地を基本とし、ショtJ360g/l
、pH5.8の液体培地を用い、オーキシンとしてIB
A10−6?l 、サイトカイニンとしてB八を3X1
0−&M、抗ジベレリンとしてアンシミドールを10−
hMを添加した.これを市販のカルチャーボックス(容
禎約1000atg,ふたにシリコンチューブを取り付
け、ガス用ろ過フィルターをつけたもの)にポリエステ
ルウール約2.0gとともに各180d分注した後、上
記アスパラガス茎切片を100個置床して、25゜C、
30001ux ,通気速度30m/+winの条件下
で4週間培養したところ表1に示す結果を得た。The medium is based on ASP medium, shot J360g/l
, using a liquid medium with pH 5.8, and using IB as auxin.
A10-6? l, 3X1 B8 as cytokinin
0-&M, 10- and uncimidol as anti-gibberellin
hM was added. After dispensing 180 d each of this together with about 2.0 g of polyester wool into a commercially available culture box (approximately 1000 atg, with a silicone tube attached to the lid and a gas filtration filter), 100 Individually placed on the floor at 25°C.
When cultured for 4 weeks under the conditions of 30,001 ux and an aeration rate of 30 m/+win, the results shown in Table 1 were obtained.
比較例1
実施例1において、空気通気を行わないこと以外は実施
例1と同様にして行った結果、表lに示す結果を得た。Comparative Example 1 The same procedure as in Example 1 was performed except that air ventilation was not performed, and as a result, the results shown in Table 1 were obtained.
実施例2
実施例1の手法により得られたアスパラガスの品種“北
海100”のクラウンを2〜3芽を含むように切り分け
たものを材料とした。培地はASP培地を基本とし、シ
−yl!60g/J!SpH5.7とし、これにオーキ
シンとしてIBA @10−’M 、サイトカイニンと
してB^を10−’M 、抗ジベレリンとしてアンシミ
ドールを10−’M添加した。これを市販のカルチャー
ボックス(容積約1000rd,ふたにシリコンチュー
ブを取り付け、ガス用ろ過フィルターをつけたもの)に
ポリエステルウール約3.0gとともに各180d分注
した後、上記アスパラガスクラウンを25個/BOXず
つ計100個置床して、25℃、3000]tv 、空
気通気速度30d/+ginの条件下で4週間培養した
ところ表2に示す結果を得た。Example 2 The crown of the asparagus cultivar "Hokkai 100" obtained by the method of Example 1 was cut into pieces containing 2 to 3 buds as a material. The medium is basically ASP medium, and C-yl! 60g/J! The pH was adjusted to 5.7, and to this were added IBA@10-'M as auxin, 10-'M B^ as cytokinin, and 10-'M of ancymidol as anti-gibberellin. After dispensing 180 d each of this together with about 3.0 g of polyester wool into a commercially available culture box (capacity approx. 1000 rd, with a silicone tube attached to the lid and a gas filtration filter), 25 asparagus crowns/ A total of 100 boxes were placed on a bed and cultured for 4 weeks under the conditions of 25° C., 3000] tv and an air aeration rate of 30 d/+gin. The results shown in Table 2 were obtained.
比較例2
実施例2において、空気通気を行わないこと以外は実施
例2と同様にして行った結果、表2に示す結果を得た。Comparative Example 2 Example 2 was carried out in the same manner as in Example 2 except that air ventilation was not performed, and the results shown in Table 2 were obtained.
実施例3
実施例2の手法により維持しているアスパラガスの品種
“北海100”のクラウンを2〜3芽を含むように切り
分けたものを材料とした。培地はASP培地を基本とし
、シ!F 1!60g/ l , pH5. 7とし
、これにオーキシンとしてIBMを10−’M 、サイ
トカイニンとしてBAを10−’?l ,抗ジベレリン
としてアンシミドールをIO−″H添加した。これを市
販のカルチャーボックス(容積約1000d、ふたにシ
リコンチューブを取り付け、ガス用ろ過フィルターをつ
けたものに)にポリエステルウール約3. 0gととも
に各180一分注した後、上記アスパラガスクラウンを
25個/BOXずつ計100個置床して、25゜C、3
000]IJX ,空気通気速度3 0 d / m
i nの条件下で4週間培養したところ表3に示す結果
を得た。Example 3 The crown of the asparagus variety "Hokkai 100" maintained by the method of Example 2 was cut into pieces containing 2 to 3 buds and used as a material. The medium is basically ASP medium. F 1!60g/l, pH5. 7, IBM as auxin at 10-'M, and BA as cytokinin at 10-'? 1, IO-''H of uncimidol was added as an anti-gibberellin. This was placed in a commercially available culture box (capacity: approximately 1000 d, with a silicone tube attached to the lid and a gas filtration filter attached), and about 3.0 mm of polyester wool was added. After dispensing 180 pieces each with 0g, place the above asparagus crowns on a bed of 25 pieces/BOX for a total of 100 pieces, and heat at 25°C for 3 hours.
000]IJX, air ventilation rate 30 d/m
When cultured for 4 weeks under conditions of i.n., the results shown in Table 3 were obtained.
比較例3
実施例3において、空気通気を行わないこと以外は実施
例3と同様にして行った結果、表3に示す結果を得た。Comparative Example 3 Example 3 was carried out in the same manner as in Example 3 except that air ventilation was not performed, and the results shown in Table 3 were obtained.
(本頁以下余白)
表 1
実施例1
比較例1
クラウン形或率
85%
62%
表3
発根率 平均生重/発根個体
実施例3 76% 195■
比較例3 34% 180mg表2
実施例2
比較例2
クラウン増殖率
4.3倍
3.5倍
(本頁以下余白)
〔発明の効果〕
本発明の方法によれば、組織培養により、アスパラガス
属植物からクラウンを形成させ、また、これを効率的に
大量増殖することができ、さらに、得られたクラウンを
経由して植物体及び種苗を大量に効率良く増殖させるこ
とができる。(Margin below this page) Table 1 Example 1 Comparative Example 1 Crown shape coverage rate 85% 62% Table 3 Rooting rate Average fresh weight/rooted individual Example 3 76% 195■ Comparative Example 3 34% 180mgTable 2 Implementation Example 2 Comparative Example 2 Crown multiplication rate 4.3 times 3.5 times (blank space below this page) [Effects of the invention] According to the method of the present invention, crowns are formed from Asparagus plants by tissue culture, and , this can be efficiently propagated in large quantities, and furthermore, plants and seedlings can be efficiently propagated in large quantities via the obtained crowns.
第1図は、アスパラガス属植物のクラウン形戊、増殖、
植物体の増殖等のプロセスを示す図、第2図は、クラウ
ン形或に使用する培養装置である。
第
1
図Figure 1 shows the crown shape, proliferation, and growth of Asparagus plants.
FIG. 2, which is a diagram showing processes such as propagation of plants, is a culture device used for crown-shaped plants. Figure 1
Claims (1)
浸した液体培地を用い通気条件下で培養してクラウンを
形成させることを特徴とするアスパラガス属植物のクラ
ウン形成方法。 2、請求項1記載の方法で形成させたクラウンを切断し
て得た切片をさらに支持材に含浸した液体培地を用い通
気条件下で培養してクラウンを肥大させ、さらに必要に
応じて同様のクラウン切断→切片培養→クラウン肥大の
増殖プロセスを繰り返すことを特徴とするアスパラガス
属植物のクラウン増殖方法。 3、請求項1又は請求項2記載の方法で得られたクラウ
ンを支持材に含浸した液体培地を用い通気条件下で培養
して植物体を生育させることを特徴とするアスパラガス
属植物の植物体の増殖方法。 4、植物体の生育工程に発根工程を含むことを特徴とす
る請求項3記載のアスパラガス属植物の植物体の増殖方
法。 5、請求項3又は4記載の方法で得られた植物体を馴化
せしめて種苗とすることを特徴とするアスパラガス属植
物の種苗増殖方法。[Claims] 1. A method for forming a crown of a plant of the genus Asparagus, which comprises forming a crown by culturing under aerated conditions using a liquid medium in which a supporting material is impregnated with tissue or callus of a plant of the genus Asparagus. . 2. The sections obtained by cutting the crown formed by the method described in claim 1 are further cultured under aerated conditions using a liquid medium impregnated with a supporting material to enlarge the crown, and if necessary, the same A method for propagating crowns of plants of the genus Asparagus, which is characterized by repeating the propagation process of crown cutting → section culture → crown enlargement. 3. A plant of the genus Asparagus, characterized in that the plant is grown by culturing it under aerated conditions using a liquid medium in which a support material is impregnated with the crown obtained by the method according to claim 1 or claim 2. How the body multiplies. 4. The method for propagating a plant of the genus Asparagus according to claim 3, wherein the growth step of the plant includes a rooting step. 5. A method for propagating seeds and seedlings of plants of the genus Asparagus, which comprises acclimatizing the plants obtained by the method according to claim 3 or 4 to produce seedlings.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1154607A JPH0322930A (en) | 1989-06-19 | 1989-06-19 | Formation and proliferation of crown of plant of genus asparagus and proliferation of seedling |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1154607A JPH0322930A (en) | 1989-06-19 | 1989-06-19 | Formation and proliferation of crown of plant of genus asparagus and proliferation of seedling |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH0322930A true JPH0322930A (en) | 1991-01-31 |
Family
ID=15587879
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1154607A Pending JPH0322930A (en) | 1989-06-19 | 1989-06-19 | Formation and proliferation of crown of plant of genus asparagus and proliferation of seedling |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH0322930A (en) |
-
1989
- 1989-06-19 JP JP1154607A patent/JPH0322930A/en active Pending
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