JPH03215569A - Coloring agent and aromatizing agent comprising extract derived from leaf of eucommia ulmoides - Google Patents

Abstract

PURPOSE:To obtain a pitch-black coloring agent or an aromatizing agent having a mellow aroma comprising an extract derived from the leaves of Eucommia ulmoides by treating a liquid prepared by extracting dry leaves of Eucommia ulmoides with hot water. CONSTITUTION:The leaves of Eucommia ulmoides picked before being shed are dried in air and water is added thereto to conduct extraction for, e.g. about 20min at 100 deg.C. The extraction residue is removed and washed with water several times in order to completely extract the ingredient. The extract is combined with the washings and passed through an adsorption column to allow the adsorbent to adsorb the ingredient contained in the solution, and a desorption solvent is passed through the column to desorb the ingredients from the adsorbent. An ion exchange resin can be used as the adsorbent. A dilute acid solution or a dilute alkali solution is used as the desorption solvent; a dilute alkali solution is desirable for obtaining a pitch-black extract, and a dilute acid solution is desirable for obtaining an aromatic extract. The eluate is concentrated. The extract thus obtained from the leaves of Eucommia ulmoides contains an ingredient having a mellow aroma and a pitch-black color characteristic of Eucommia ulmoides.

Description

[Detailed description of the invention] [Industrial application field] This invention is based on Eucommia ulmoide.
The present invention relates to a new use of the extracted extract obtained from s), and more specifically, to a coloring agent and aromatic agent made from an extract derived from Duchu leaf.

[Prior Art and Problems to be Solved] The bark of Eucommia, a deciduous plant native to China, is listed in the Elements of the Shennong Bencaojing as a herbal medicine. Currently, most of the shazhong trees used as herbal medicines are grown naturally or cultivated in mainland China and other countries, and the bark is obtained by cutting down mature trees that are about 20 years old and peeling off their bark. is used as a medicinal raw material.

According to the Encyclopedia of Traditional Chinese Medicine and the Pharmacy Dictionary of the People's Republic of China, the medicinal properties of Shazhong are as follows: ``It has the effect of replenishing the kidneys and liver, strengthening the muscles and bones, and calming the womb. It is said to cure urine, abnormal bleeding in women, premature miscarriage, and high blood pressure, and numerous medicinal properties have been reported. however,
It takes a lot of effort to cut down trees and peel the bark.
Also, collecting large amounts of bark has a negative impact on plant survival and growth.

On the other hand, leaves have the advantage that they can be harvested repeatedly with simple operations, and tea prepared from dried leaves has a luster close to gold and a mellow aroma.

As a result of intensive research, the present inventors previously discovered that the medicinal components contained in the bark of Morifolia are also contained in the leaves of Morifolia (
During the process of preparing medicinal ingredients, the inventors discovered a new extract fraction that exhibits a jet-black evening color and an extract fraction that produces a fragrance, leading to the completion of the present invention.

The present invention uses leaves, which are materials that can be repeatedly collected with simple operations, as an extraction source, and extracts them by extracting them. The purpose is to provide.

The extract provided by the present invention can impart the rich aroma and jet black color unique to Shachu to various foods and articles.
Becomes Noh.

[Means for Solving the Problems 1] The extract derived from Moriflora leaves according to the present invention is characterized in that it is prepared from the extract obtained by extracting leaves of the Moriflora plant.

Here, as the raw material for extraction, it is necessary that it can be easily and repeatedly obtained in large quantities, so current year's leaves are suitable. However, the raw materials are not limited to these. In addition, shachu is a deciduous plant, and collecting autumn leaves is
Unlike collecting bark, this method is advantageous in that it does not cause damage to the trees and it is easy to secure a large amount of material.

However, this is not limited either. Shachu leaves are naturally dried before extraction.

A typical example of a solvent for extraction is water, especially heated water.

Other extraction solvents include lower alcohols such as methanol and ethanol, ketones such as acetone and methyl ethyl ketone, aromatic hydrocarbons such as benzene and toluene, ethers such as diethyl ether and dioxane, chloroform and Examples include organic solvents such as halogenated hydrocarbons such as carbon tetrachloride and heterocyclic compounds such as pyridine. These solvents may be used alone or in combination in two or more cases. In order to make the obtained extract drinkable, water is preferable as the extraction solvent.

Extraction is usually carried out under heat, but may also be carried out at room temperature. The more times the extraction is performed, the better, but usually it is several times.

To prepare an extract from an extract, the components contained in the extract are adsorbed onto an adsorbent by passing the extract through an adsorption column, and then the same components are desorbed from the adsorbent by passing a desorption solvent through the same column. let Various ion exchange resins are used as the adsorbent. Further, a typical example of the desorption solvent is a dilute acid or dilute alkaline solution. As other desorption solvents, the above extraction solvents are used. Dilute alkali is preferred to obtain a jet black extract, and dilute acid is preferred to obtain an aromatic extract.

Furthermore, when the obtained extract is to be used for drinking, dilute acids or dilute alkalis are preferred, and these are neutralized after extraction.

The desorption liquid is then concentrated and the concentrate is dried.

Thus, the extract according to the present invention obtained from the leaves of Shachu contains the rich aroma derived from Shachu and components that give it a jet black color.

Analysis such as isolation and identification of the above components can be performed using gas chromatography, high performance liquid chromatography, solvent extraction, thin layer chromatography (TLC), nuclear magnetic resonance spectroscopy (N
MR), ultraviolet absorption spectrum (UV), infrared absorption spectrum (IR), mass spectrometry (M
This is done using a method such as S).

These components are naturally contained in the raw material leaf, and it goes without saying that even if the powder obtained by pulverizing the raw material leaf is used without going through the above extraction operation, it still functions as a coloring agent.

[Example] The present invention will now be specifically explained with reference to Examples.

Example 1 a) Extract production method Morifolia leaves, which were picked before falling, were naturally dried until the moisture content became 10% or less. Distilled water or 10 parts of boiled distilled water was added to 1 kg of this Mori leaves, and extraction was performed at 100° C. for 20 minutes.

The extraction residue (from the tea) was removed with a stainless steel mesh (mesh size 250 μm), and the residue was washed three times with 10% distilled water to completely extract the components.

The extract solution and washing solution were combined and filtered using an ultrafiltration device (Nitto Denko Corporation).
Membrane Master RUW-4 manufactured by the same company, NTU- manufactured by the same company
3508-pl8LP (tubular type)) and reverse osmosis (RO) membrane: NTR.
Using -7250-S4 (spiral type),
The extract was concentrated to obtain 5 liters of concentrated extract.

Next, the mixture was passed through a weakly basic ion exchange resin Amberly I RA-9 3 resin, which had been previously activated and packed in a glass column with an inner diameter of 3 cm and a length of 30 cm, to adsorb the aromatic components. After washing the resin with distilled water, dilute alkali (0
．． Extract 20% by elution with 2N caustic soda)
0 ml was obtained.

b) Properties of the extract Regarding the obtained extract, from 400 nm to 800 nm
Absorption to nm light was investigated. The results are shown in FIG. Also, for reference, the absorption pattern of Shachu extract (tea) without undergoing fractionation is shown in Figure 2. Through the fractionation operation, substances having absorption at 500 nm were concentrated. FIG. 3 is an enlarged version of FIG. 70 derived from red
0-618 nm, 686-571 nm from yellow color
, there is an absorption of 482 to 435 nm derived from blue,
A jet-black sample, which is rare for biological materials, was obtained. FIG. 4 shows the absorption pattern of commercially available black fountain pen ink for comparison. In FIG. 1, absorption is observed at 400 nm or less, but these are derived from lignans and iridoids, which are contained in large quantities in the company. Also,
A high performance liquid chromatograph of the obtained extract is shown in FIG.

There is no jet black pigment as a food additive, and by adding this extract, it is possible to produce black ice cream, jelly pudding, etc. By implementing the present invention, we will provide materials that will stimulate new consumer demand. Is possible.

Example 2 200 ml of aromatic extract was obtained by using dilute acid (0.2N hydrochloric acid) instead of alkali as a desorption solvent and performing other operations in the same manner as in Example 1.

The obtained aromatic extract was analyzed by thin layer chromatography using a developing solvent having a composition of chloroformmethanol:water=70:30:5. The results of this analysis are shown in FIG.

As is clear from the figure, this extract contains 0.1,
Multiple components are included with Rf values of 0.25, 0.37, 0.42, 0.58, 0.77 and 0.83. Furthermore, a high performance liquid chromatograph of the obtained extract is shown in FIG.

Sensory Test The obtained aromatic extract was subjected to a sensory test by five panel members. The test results are shown in Table 1.

(Left below) Table 1 As is clear from the table, all five panel members reported a refreshing feeling similar to that of camphor.

[Brief explanation of drawings]

FIG. 1 shows an absorption pattern of a jet black extract obtained in an example of the present invention. FIG. 2 shows the absorption pattern of Mori extract (tea) without undergoing fractionation. FIG. 3 is an enlarged view of FIG. 1. FIG. 4 shows the absorption pattern of commercially available black ink for fountain pens. Figure 5 is a high performance liquid chromatograph of an extract containing jet black components. FIG. 6 is a development view of the aromatic extract obtained according to the example of the present invention, obtained by thin layer chromatography analysis. FIG. 7 is a high performance liquid chromatograph of the aroma extract. that's all

Claims (2)

[Claims] (1) A coloring agent made of an extract prepared from a hot water extract of dried Duchu leaves. (2) A fragrance made from an extract prepared from a hot water extract of dried Mori leaves.