JPH0320770Y2 - - Google Patents

Description

[Detailed description of the invention] (a) Industrial application field This invention is used to remove air bubbles that adhere to the inner wall of the electrophoresis tube and the inner side of the connection between the electrophoresis tube and other members in a capillary isokinetic electrophoresis device. Regarding structure.

(B) Prior Art Generally, it is known that during electrophoresis, if air bubbles adhere to the above-mentioned areas, they will interfere with analysis.

These bubbles cannot be removed simply by feeding the electrolyte into the migration tube, so conventionally, a shock was applied to the migration tube by hitting the migration tube and a detector, which is one of the other components installed in the migration tube. to remove air bubbles.

(c) Problems to be solved by the invention However, with the above method, it was not possible to confirm that the air bubbles had been reliably removed, so analysis was sometimes performed with the air bubbles still present. In this case,
Since the analysis had to be repeated, the analysis time became longer and the electrolyte solution was wasted.

This idea was made in view of the above circumstances,
The object of this invention is to enable confirmation that air bubbles have been reliably removed from the electrophoresis section, thereby preventing waste of analysis time and electrolyte solution.

(d) Means to solve the problem This device is a capillary type isotachophoresis device,
A constant current electrophoresis power supply circuit, a terminal electrode tank and a leading electrode tank connected to both ends of this migration power supply circuit, an electrophoresis tube that connects these two electrode tanks, and a terminal electrode tank side of this migration tube and a leading electrode. a sample injection section and a detector respectively interposed on the tank side; a drain path connected to a portion of the migration tube near the sample injection section via a channel opening/closing valve; and a channel opening/closing valve operating means; A supply path for supplying a terminal liquid and a leading liquid into each electrode tank, a liquid pump provided in each of these supply paths, a voltage monitor means for monitoring the electrophoresis voltage from the electrophoresis power supply circuit, and a voltage monitoring means for monitoring the electrophoresis voltage from the electrophoresis power supply circuit. exceeds a preset set voltage value, outputs a energization stop signal to the electrophoresis power supply circuit, outputs an activation signal to either one of the liquid pumps, and further outputs a flow path open signal to the flow path opening/closing valve operating means. and a control means for alternately outputting a channel blockage signal for a predetermined period of time.

(E) Effect This device stops energizing the migration tube and activates one of the liquid pumps when the monitor voltage exceeds a preset voltage value.
Further, the flow path opening/closing valve is alternately opened and closed for a predetermined period of time.

(f) Examples This invention will be described in detail below based on examples shown in the figures. Note that this invention is not limited to this.

In FIG. 1, a capillary electrophoresis device 1 includes a migration power supply circuit 2, a terminal electrode tank 3 and a leading electrode tank 4 connected to both ends of this circuit 2.
and a migration tube 5 that connects these two electrode tanks 3 and 4.
The electrophoresis tube is mainly composed of a sample injection section 6 and a detector 7, which are interposed on the terminal electrode tank 3 side and the leading electrode tank 4 side, respectively.

A drain path 8 is connected to a portion of the migration tube 5 near the sample injection portion 6, and a flow path opening/closing valve 9 is interposed in the drain path 8. On the other hand, supply paths 10 and 11 are connected to each of the electrode tanks 3 and 4, respectively, for supplying a terminal liquid and a leading liquid to each of these electrode tanks 3 and 4. are liquid sending pumps 12 and 13, respectively.
is provided. In addition, the liquid sending pumps 12, 13
For example, a straining pump is used.

A voltage monitor means 14 for monitoring the electrophoresis voltage is electrically connected to the migration power supply circuit 2, and a pulse motor 15 as a flow path opening/closing valve operating means is electrically connected to the flow path opening/closing valve 9. The on-off valve 9 is equipped with a pulse motor 15 as a means for operating the flow path on-off valve.
is electrically connected. This pulse motor 15,
A control means 16 is electrically connected to the voltage monitor means 14, the electrophoresis power supply circuit 2, and both liquid feeding pumps 12 and 13. This control means 16 is composed of, for example, a microcomputer. In addition, 17, 18
is an electrode.

Next, the operation of the device 1 will be explained.

When a constant current is applied to the electrophoresis tube 5 to start electrophoresis, the electrophoresis voltage increases with the passage of time. This voltage change is monitored by voltage monitoring means 14.
At this time, if bubbles are present in the electrophoresis tube 5, the electrophoresis voltage increases more rapidly than a normal voltage increase.
Therefore, the monitor voltage is set to a preset voltage value,
For example, when the voltage exceeds 15~20KV, control means 1
6 outputs an energization stop signal to the electrophoresis power supply circuit 2, outputs an activation signal to either one of the liquid pumps 12, 13, and alternately outputs a flow path open signal and a flow path blockage signal to the pulse motor 15. The channel opening/closing valve 9 is opened and closed. In this case, when the channel opening/closing valve 9 is closed, the inside of the migration tube 5 becomes pressurized and pressure is applied to the bubbles. Next, when the channel opening/closing valve 9 is opened, the pressure inside the migration tube 5 returns to its original state, and at the same time, the bubbles are moved. By repeating this for about one minute, the bubbles are discharged from the migration tube 5 to the drain path 8. Thereafter, a terminal liquid and a leading liquid are fed to the electrophoresis tube 5 as in the conventional case, and analysis is started. Therefore, analysis can be performed accurately without wasting the electrolyte solution.

(g) Effects of the device This device sends the leading liquid or terminal liquid to each electrode tank and alternately opens and closes the channel opening/closing valve only when the monitored voltage exceeds a preset voltage value. Since air bubbles are removed in this manner, it is possible to prevent wastage of leading liquid or terminal liquid and analysis time.

[Brief explanation of the drawing]

FIG. 1 is a configuration explanatory diagram showing an embodiment of a capillary isokinetic electrophoresis device according to this invention. DESCRIPTION OF SYMBOLS 1... Capillary isotachophoresis device, 2... Electrophoresis power supply circuit, 3... Terminal electrode tank, 4... Leading electrode tank, 5... Electrophoresis tube, 6... Sample injection part, 7... Detector , 8...Drainage path, 9...Flow path opening/closing valve, 10, 11...Liquid feeding path, 12, 13...Liquid feeding pump, 14...Voltage monitoring means, 15...Pulse motor (flow path opening/closing) valve actuation means), 16... control means.

Claims (1)

[Scope of utility model registration request] A constant current electrophoresis power supply circuit, a terminal electrode tank and a leading electrode tank connected to both ends of this migration power supply circuit, an electrophoresis tube that connects these two electrode tanks, and a terminal electrode tank side of this migration tube and a leading electrode. a sample injection section and a detector respectively interposed on the tank side; a drain path connected to a portion of the migration tube near the sample injection section via a channel opening/closing valve; and a channel opening/closing valve operating means; A supply path for supplying a terminal liquid and a leading liquid into each electrode tank, a liquid pump provided in each of these supply paths, a voltage monitor means for monitoring the electrophoresis voltage from the electrophoresis power supply circuit, and a voltage monitoring means for monitoring the electrophoresis voltage from the electrophoresis power supply circuit. exceeds a preset set voltage value, outputs a energization stop signal to the electrophoresis power supply circuit, outputs an activation signal to either one of the liquid pumps, and further outputs a flow path open signal to the flow path opening/closing valve operating means. and a control means for alternately outputting a channel occlusion signal for a predetermined period of time.