JPH03201977A - Culture of microorganism in blood and culture medium used for method thereof - Google Patents
Culture of microorganism in blood and culture medium used for method thereofInfo
- Publication number
- JPH03201977A JPH03201977A JP34076789A JP34076789A JPH03201977A JP H03201977 A JPH03201977 A JP H03201977A JP 34076789 A JP34076789 A JP 34076789A JP 34076789 A JP34076789 A JP 34076789A JP H03201977 A JPH03201977 A JP H03201977A
- Authority
- JP
- Japan
- Prior art keywords
- zeta potential
- blood
- potential filter
- microorganisms
- filter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000008280 blood Substances 0.000 title claims abstract description 31
- 210000004369 blood Anatomy 0.000 title claims abstract description 31
- 244000005700 microbiome Species 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims description 14
- 239000001963 growth medium Substances 0.000 title claims description 3
- 229940079593 drug Drugs 0.000 claims abstract description 22
- 239000003814 drug Substances 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000011358 absorbing material Substances 0.000 claims abstract description 9
- 239000012503 blood component Substances 0.000 claims abstract description 8
- 238000012258 culturing Methods 0.000 claims description 11
- 230000002745 absorbent Effects 0.000 claims description 8
- 239000002250 absorbent Substances 0.000 claims description 8
- 239000002609 medium Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 6
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 239000006161 blood agar Substances 0.000 description 3
- XIURVHNZVLADCM-IUODEOHRSA-N cefalotin Chemical compound N([C@H]1[C@@H]2N(C1=O)C(=C(CS2)COC(=O)C)C(O)=O)C(=O)CC1=CC=CS1 XIURVHNZVLADCM-IUODEOHRSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 2
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 2
- 241000588722 Escherichia Species 0.000 description 2
- 229930182566 Gentamicin Natural products 0.000 description 2
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 2
- 241000607720 Serratia Species 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- 229960000603 cefalotin Drugs 0.000 description 2
- 229940106164 cephalexin Drugs 0.000 description 2
- ZAIPMKNFIOOWCQ-UEKVPHQBSA-N cephalexin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@@H]3N(C2=O)C(=C(CS3)C)C(O)=O)=CC=CC=C1 ZAIPMKNFIOOWCQ-UEKVPHQBSA-N 0.000 description 2
- 239000007330 chocolate agar Substances 0.000 description 2
- 229960002518 gentamicin Drugs 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- -1 Cephalozin Chemical compound 0.000 description 1
- GSDSWSVVBLHKDQ-UHFFFAOYSA-N 9-fluoro-3-methyl-10-(4-methylpiperazin-1-yl)-7-oxo-2,3-dihydro-7H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid Chemical compound FC1=CC(C(C(C(O)=O)=C2)=O)=C3N2C(C)COC3=C1N1CCN(C)CC1 GSDSWSVVBLHKDQ-UHFFFAOYSA-N 0.000 description 1
- 241000607534 Aeromonas Species 0.000 description 1
- 241001148536 Bacteroides sp. Species 0.000 description 1
- 241000193403 Clostridium Species 0.000 description 1
- 108010078777 Colistin Proteins 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000588914 Enterobacter Species 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 241000606790 Haemophilus Species 0.000 description 1
- 241000186781 Listeria Species 0.000 description 1
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 241000186429 Propionibacterium Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- WKDDRNSBRWANNC-UHFFFAOYSA-N Thienamycin Natural products C1C(SCCN)=C(C(O)=O)N2C(=O)C(C(O)C)C21 WKDDRNSBRWANNC-UHFFFAOYSA-N 0.000 description 1
- 229960004821 amikacin Drugs 0.000 description 1
- LKCWBDHBTVXHDL-RMDFUYIESA-N amikacin Chemical compound O([C@@H]1[C@@H](N)C[C@H]([C@@H]([C@H]1O)O[C@@H]1[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O1)O)NC(=O)[C@@H](O)CCN)[C@H]1O[C@H](CN)[C@@H](O)[C@H](O)[C@H]1O LKCWBDHBTVXHDL-RMDFUYIESA-N 0.000 description 1
- 229960003644 aztreonam Drugs 0.000 description 1
- WZPBZJONDBGPKJ-VEHQQRBSSA-N aztreonam Chemical compound O=C1N(S([O-])(=O)=O)[C@@H](C)[C@@H]1NC(=O)C(=N/OC(C)(C)C(O)=O)\C1=CSC([NH3+])=N1 WZPBZJONDBGPKJ-VEHQQRBSSA-N 0.000 description 1
- 229960003669 carbenicillin Drugs 0.000 description 1
- FPPNZSSZRUTDAP-UWFZAAFLSA-N carbenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)C(C(O)=O)C1=CC=CC=C1 FPPNZSSZRUTDAP-UWFZAAFLSA-N 0.000 description 1
- 229960002682 cefoxitin Drugs 0.000 description 1
- WZOZEZRFJCJXNZ-ZBFHGGJFSA-N cefoxitin Chemical compound N([C@]1(OC)C(N2C(=C(COC(N)=O)CS[C@@H]21)C(O)=O)=O)C(=O)CC1=CC=CS1 WZOZEZRFJCJXNZ-ZBFHGGJFSA-N 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229960002227 clindamycin Drugs 0.000 description 1
- KDLRVYVGXIQJDK-AWPVFWJPSA-N clindamycin Chemical compound CN1C[C@H](CCC)C[C@H]1C(=O)N[C@H]([C@H](C)Cl)[C@@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](SC)O1 KDLRVYVGXIQJDK-AWPVFWJPSA-N 0.000 description 1
- 229960003346 colistin Drugs 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229960003276 erythromycin Drugs 0.000 description 1
- 229960002182 imipenem Drugs 0.000 description 1
- ZSKVGTPCRGIANV-ZXFLCMHBSA-N imipenem Chemical compound C1C(SCC\N=C\N)=C(C(O)=O)N2C(=O)[C@H]([C@H](O)C)[C@H]21 ZSKVGTPCRGIANV-ZXFLCMHBSA-N 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 229960003085 meticillin Drugs 0.000 description 1
- JORAUNFTUVJTNG-BSTBCYLQSA-N n-[(2s)-4-amino-1-[[(2s,3r)-1-[[(2s)-4-amino-1-oxo-1-[[(3s,6s,9s,12s,15r,18s,21s)-6,9,18-tris(2-aminoethyl)-3-[(1r)-1-hydroxyethyl]-12,15-bis(2-methylpropyl)-2,5,8,11,14,17,20-heptaoxo-1,4,7,10,13,16,19-heptazacyclotricos-21-yl]amino]butan-2-yl]amino]-3-h Chemical compound CC(C)CCCCC(=O)N[C@@H](CCN)C(=O)N[C@H]([C@@H](C)O)CN[C@@H](CCN)C(=O)N[C@H]1CCNC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCN)NC(=O)[C@H](CCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CCN)NC1=O.CCC(C)CCCCC(=O)N[C@@H](CCN)C(=O)N[C@H]([C@@H](C)O)CN[C@@H](CCN)C(=O)N[C@H]1CCNC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCN)NC(=O)[C@H](CCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](CC(C)C)NC(=O)[C@H](CCN)NC1=O JORAUNFTUVJTNG-BSTBCYLQSA-N 0.000 description 1
- 229960001699 ofloxacin Drugs 0.000 description 1
- 229960001019 oxacillin Drugs 0.000 description 1
- UWYHMGVUTGAWSP-JKIFEVAISA-N oxacillin Chemical compound N([C@@H]1C(N2[C@H](C(C)(C)S[C@@H]21)C(O)=O)=O)C(=O)C1=C(C)ON=C1C1=CC=CC=C1 UWYHMGVUTGAWSP-JKIFEVAISA-N 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 108700018363 polymyxin B(1) Proteins 0.000 description 1
- WQVJHHACXVLGBL-GOVYWFKWSA-N polymyxin B1 Polymers N1C(=O)[C@H](CCN)NC(=O)[C@@H](NC(=O)[C@H](CCN)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCN)NC(=O)CCCC[C@H](C)CC)CCNC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCN)NC(=O)[C@H](CCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1CC1=CC=CC=C1 WQVJHHACXVLGBL-GOVYWFKWSA-N 0.000 description 1
- XDJYMJULXQKGMM-UHFFFAOYSA-N polymyxin E1 Natural products CCC(C)CCCCC(=O)NC(CCN)C(=O)NC(C(C)O)C(=O)NC(CCN)C(=O)NC1CCNC(=O)C(C(C)O)NC(=O)C(CCN)NC(=O)C(CCN)NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)C(CCN)NC1=O XDJYMJULXQKGMM-UHFFFAOYSA-N 0.000 description 1
- KNIWPHSUTGNZST-UHFFFAOYSA-N polymyxin E2 Natural products CC(C)CCCCC(=O)NC(CCN)C(=O)NC(C(C)O)C(=O)NC(CCN)C(=O)NC1CCNC(=O)C(C(C)O)NC(=O)C(CCN)NC(=O)C(CCN)NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)C(CCN)NC1=O KNIWPHSUTGNZST-UHFFFAOYSA-N 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229910052594 sapphire Inorganic materials 0.000 description 1
- 239000010980 sapphire Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 229960004659 ticarcillin Drugs 0.000 description 1
- OHKOGUYZJXTSFX-KZFFXBSXSA-N ticarcillin Chemical compound C=1([C@@H](C(O)=O)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)C=CSC=1 OHKOGUYZJXTSFX-KZFFXBSXSA-N 0.000 description 1
- 229960000707 tobramycin Drugs 0.000 description 1
- NLVFBUXFDBBNBW-PBSUHMDJSA-N tobramycin Chemical compound N[C@@H]1C[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N NLVFBUXFDBBNBW-PBSUHMDJSA-N 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
この出願の発明は、血液中の微生物を培養する方法、及
びその方法に用いる培養器に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The invention of this application relates to a method for culturing microorganisms in blood and an incubator used in the method.
従来、血液中の微生物を培養及び分離するには、採取し
た血液を液体培地と混合して培養し、この培地中で増殖
した微生物を血液寒天やチョコレート寒天等の分離培地
により分離培養をしていた［発明が解決しようとする課
題］
しかしながら、血液中に抗菌剤等の薬剤が含まれる場合
には、採取した血液を液体培地と混合しても、その薬剤
の影響により血液中の微生物が増殖するのが非常に困難
であるという課題を有していた。Conventionally, in order to culture and isolate microorganisms in blood, collected blood is mixed with a liquid medium and cultured, and the microorganisms grown in this medium are isolated and cultured using a separation medium such as blood agar or chocolate agar. [Problems to be Solved by the Invention] However, if blood contains drugs such as antibacterial agents, even if the collected blood is mixed with a liquid medium, microorganisms in the blood may proliferate due to the effects of the drugs. The problem was that it was extremely difficult to do so.
そこで、この出願の発明は、血液中に抗菌剤等の薬剤が
含まれていても、採取した血液中の微生物の増殖を容易
にする血中微生物培養方法及びその方法に用いる培養器
を提供することを目的とする。Therefore, the invention of this application provides a method for culturing microorganisms in blood that facilitates the growth of microorganisms in collected blood even if the blood contains drugs such as antibacterial agents, and an incubator used in the method. The purpose is to
〔課題を解決するための手段］
そのため、この出願の発明の血中微生物培養方法は、薬
剤を含む血液中の微生物をゼータ電位フィルターに吸着
させると共に、ゼータ電位フィルターから薬剤及び血液
成分を除去したのち、ゼータ電位フィルターに吸着させ
た微生物を培養するようにしている。[Means for Solving the Problems] Therefore, the method for culturing blood microorganisms of the invention of this application adsorbs microorganisms in blood containing drugs to a zeta potential filter, and removes drugs and blood components from the zeta potential filter. Later, the microorganisms adsorbed to the zeta potential filter are cultured.
さらに、この出願の発明の血中微生物培養方法に用いる
培養器は、吸水体を一部に接触させたゼータ電位フィル
ターを備えると共に、このゼータ電位フィルターの吸水
体を接触させていない部分を切り落とす切刃を備え、さ
らにこのゼータ電位フィルターの下方に培地を備えたも
のとしているこの出願の発明において、血液中に含まれ
る薬剤としては、アンピシリン、カルベニシリン、チカ
ルシリン、ペニシリン６１メチシリン、オキサシリン、
セファロジン、セファロチン、セファレキシン、セフオ
キシチン、セファレキシン、アズスレオナム、カナマイ
シン、ゲンタマイシン、アミカシン、ストレプトマイシ
ン、トブラマイシン、イミペネム、ポリミキシンＢ１コ
リスチン、クロラムフェニコール、エリスロマイシン、
テトラサイクリン、ごノサイクリン、クリンダマイシン
、オフロキサシン、アンピシリン８１等を例示すること
ができる。Further, the incubator used in the method for culturing blood microorganisms of the invention of this application is equipped with a zeta potential filter that is partially in contact with the water absorbing material, and a portion of the zeta potential filter that is not in contact with the water absorbing material is cut off. In the invention of this application, which is provided with a blade and further provided with a medium below the zeta potential filter, the drugs contained in the blood include ampicillin, carbenicillin, ticarcillin, penicillin 61 methicillin, oxacillin,
Cephalozin, cephalothin, cephalexin, cefoxitin, cephalexin, azthreonam, kanamycin, gentamicin, amikacin, streptomycin, tobramycin, imipenem, polymyxin B1 colistin, chloramphenicol, erythromycin,
Examples include tetracycline, gonocycline, clindamycin, ofloxacin, ampicillin 81, and the like.
この出願の発明において、培養可能な微生物としては、
スタフィロコッカス（鉦鮭賎旦並匹ＵＳ Ｓｐｐ、）、
ストレプトコッカス（Ｂμ叫必遼匹並ｓｐｐ。In the invention of this application, culturable microorganisms include:
Staphylococcus (Sapphire US Spp,),
Streptococcus (Bμ shouting spp.
）、エンテロコツカス（Ｅｎｔｅｒｏｃｏｃｃｕｓ ｓ
ｐｐ、）、コリネバクテリウム（蝕すｌ山上ｔｅｒｉｕ
ｍ ｓｐｐ、） 、リステリア（Ｌｉｓｔｅｒｉａ ｓ
ｐｐ、）、ヘモフィルス（ハ悲迎ｙ吐月ｓｐｐ、） 、
サルモネラ（Ｓａｌｍｏｎｅｌｌａ ｓｐｐ、）、ニジ
エリチア（Ｅｓｃｈｅｒｉｃｈｉａ ｓｐｐ、） 、タ
レブシエラ（に１ｅｂｓｉｅｌｌａ ｓｐｐ、）、エン
テロバクタ−（ｈｔｅｒｏｂａｃｔｅｒ ｓｐｐ、）、
セラチア（Ｓｅｒｒａｔｉａ ｓｐ９．）、アエロモナ
ス（Ａｅｒｏｍｏｎａｓ ｓｐｐ、） 、シュードモナ
ス（Ｐｓｅｕｄｏｍｏｎａｓ ｓｐｐ、） 、プロピオ
ニバクテリウム（ハコおｎ）狙抜Ｕ肪ｓｐｐ、） 、ク
ロストリジウム（Ｃｌｏｓｔｒｉｄｔｕｍ ｓｐｐ、）
、バクテロイデス（Ｂａｃｔｅｒｏｉｄｅｓ ｓｐｐ
、） 、カンジダ（Ｃａｎｄｉｄａ ｓｐｐ、３等を例
示することができる。), Enterococcus s
pp, ), Corynebacterium
m spp, ), Listeria spp.
pp,), Haemophilus (Haseiyoyutsukispp,),
Salmonella spp, Escherichia spp, Talebsiella spp, Enterobacter spp,
Serratia sp9., Aeromonas spp, Pseudomonas spp, Propionibacterium spp, Clostridium spp.
, Bacteroides spp.
, ), Candida (Candida spp. 3, etc.).
この出願の発明に用いられるゼータ電位フィルターとし
ては、プラスゼータ電位フィルターまたはマイナスゼー
タ電位フィルターが使用されるが、通常、微生物細胞表
面はマイナスに荷電されているので、プラスゼータ電位
フィルターを使用すると、フィルター〇孔径より小さい
微生物がこのフィルターに吸着されることになる。また
、この発明に用いられるゼータ電位フィルターの孔径は
１〜ｌＯμｍとするのが、上記した各種の微生物を吸着
するのに好ましい。As the zeta potential filter used in the invention of this application, a positive zeta potential filter or a negative zeta potential filter is used, but since the microbial cell surface is usually negatively charged, when a positive zeta potential filter is used, Microorganisms smaller than the pore size of the filter will be adsorbed by this filter. Further, the pore diameter of the zeta potential filter used in the present invention is preferably 1 to 10 μm in order to adsorb the various microorganisms described above.
この出願の発明において、ゼータ電位フィルターから薬
剤及び血液成分を除去するには、ゼータ電位フィルター
に吸水体を接触させることにより行っており、ゼータ電
位フィルターから薬剤及び血液成分を完全に除去するの
が好ましいが（ゼータ電位フィルターに吸水体を接触さ
せるだけでは、ゼータ電位フィルターエレメント内に保
持された薬剤及び血液成分は残存する）、必ずしも完全
に除去されていなくてもよい。吸水体としては、濾紙、
脱脂綿、吸水性高分子材料等の任意のものが使用される
。In the invention of this application, drugs and blood components are removed from the zeta potential filter by bringing a water absorbent into contact with the zeta potential filter, and it is possible to completely remove drugs and blood components from the zeta potential filter. Although it is preferable (the drug and blood components retained in the zeta potential filter element remain by simply bringing the water absorbent into contact with the zeta potential filter), they do not necessarily have to be completely removed. As a water absorbent, filter paper,
Any material such as absorbent cotton or water-absorbing polymeric material can be used.
この出願の発明において、ゼータ電位フィルターに吸着
させた微生物を培養するには、通常はこのゼータ電位フ
ィルターを発育ブロス等の液体培地中に落とし込むが、
このゼータ電位フィルターを血液寒天やチョコレート寒
天等の固体培地の上に直装置いてもよい。In the invention of this application, in order to culture microorganisms adsorbed on a zeta potential filter, the zeta potential filter is usually dropped into a liquid medium such as growth broth.
This zeta potential filter may be placed directly on a solid medium such as blood agar or chocolate agar.
この出願の発明において、ゼータ電位フィルターの下面
を覆う非吸水体は、ゼータ電位フィルターが発育ブロス
等の水分を吸収しないようにしたものであり、合成樹脂
フィルム等の任意のものが使用される。In the invention of this application, the non-water absorbing material covering the lower surface of the zeta potential filter is one that prevents the zeta potential filter from absorbing moisture from growth broth, etc., and any material such as a synthetic resin film can be used.
この出願の発明の培養器を用いて、この出願の発明の血
中微生物培養方法を実施すれば、血液中に抗菌剤等の薬
剤が含まれていても、薬剤は吸水体に吸収され、微生物
はゼータ電位フィルターに吸着されるので、このゼータ
電位フィルターを液体培地等に落とし込めば、薬剤の影
響を受けず増殖することが容易にできる。If the blood microorganism culture method of the invention of this application is carried out using the incubator of the invention of this application, even if the blood contains drugs such as antibacterial agents, the drugs will be absorbed by the water absorbent and the microorganisms will be absorbed. is adsorbed by the zeta potential filter, so if this zeta potential filter is dropped into a liquid medium etc., it is possible to easily proliferate without being affected by the drug.
さらに、この出願の発明の培養器で、ゼータ電位フィル
ターの下面を非吸水体で覆ったものは、ゼータ電位フィ
ルターの下方に備えた培地が液体である場合にも、ゼー
タ電位フィルターに培地の水分が付着して、吸水体に水
分が吸収されることがないので、非常に取り扱い易くな
る。Furthermore, in the incubator of the invention of this application, in which the lower surface of the zeta potential filter is covered with a non-water-absorbing material, even if the culture medium provided below the zeta potential filter is liquid, the zeta potential filter does not contain any moisture in the medium. This prevents water from adhering to the water absorbent and being absorbed by the water absorbent body, making it extremely easy to handle.
以下、この出願の発明を実施例に基づき、具体的に説明
する。Hereinafter, the invention of this application will be specifically explained based on Examples.
試験Ｉ
ゼータ電位フィルターが、血液中の微生物を吸着できる
かどうかについて試験した。Test I The ability of the zeta potential filter to adsorb microorganisms in blood was tested.
（供試菌株）
Ｓｔａ ｈ １ｏｃｏｃｃｕｓ Ｓ、 ａｕｒｅｕｓ
（ＡＴＣＣ２５９２２）Ｓｅｒｒａｔｉａ（Ｓ、
） ｗ＋ａｒｃｅｓｃｅｎｓ （ＡＴＣＣ８１０
０）（試験方法）
（１）菌液を調製後、溶血液中に接種し、この溶血液を
ゼータ電位フィルターに通過させる。(Test strain) Stah 1ococcus S, aureus
(ATCC25922) Serratia (S,
) w+arcescens (ATCC810
0) (Test method) (1) After preparing a bacterial solution, it is inoculated into hemolysate, and the hemolysate is passed through a zeta potential filter.
（２） （１）のゼータ電位フィルターを発育ブロス中
及び５％羊血液寒天培地上に置き、３７℃で培養する。(2) Place the zeta potential filter from (1) in growth broth and on a 5% sheep blood agar medium and culture at 37°C.
（３） （２）のゼータ電位フィルター上に発育したコ
ロニーを観察する。(3) Observe the colonies that have grown on the zeta potential filter in (2).
（試験結果）
上記の表に示した通り、ゼータ電位フィルターには、血
液中の微生物を吸着することができた。(Test Results) As shown in the table above, the zeta potential filter was able to adsorb microorganisms in the blood.
試験■
ゼータ電位フィルターエレメント内に残存した薬剤が、
発育ブロス中での微生物の発育に与える影響について試
験した。Test■ The drug remaining in the zeta potential filter element is
The effect on microbial growth in growth broth was tested.
（供試菌株）
Ｓｔａ ｈ １ｏｃｏｃｃｕｓ（Ｓ、） ａｕｒｅｕｓ
（ＡＴＣＣ２５９２３）Ｅｓｃｈｅｒｉｃｈｉａ（
Ｅ、） ｃｏ￥ｉ （ＡＴＣＣ２５９２２）（
使用薬剤）
アミノベンジルペニシリン（ＡＢＰＣ）セファロチン（
ＣＥＴ）
ゲンタマイシン（ＣＭ）
（試験方法）
（１）接種用菌液（１０” ＣＦＩＩ／ｍｌ）を調製し
、この接種用菌液５鵬１を発育ブロスに接種する。(Test strain) Sta h 1ococcus (S,) aureus
(ATCC25923) Escherichia (
E,) co\i (ATCC25922) (
Drugs used) Aminobenzylpenicillin (ABPC) Cephalothin (
CET) Gentamicin (CM) (Test method) (1) Prepare a bacterial solution for inoculation (10'' CFII/ml), and inoculate 5 1 of this bacterial solution for inoculation into the growth broth.
（２）試験用薬液（１００ｕ ｇ／ｍｌ ）を調製し、
コノ試験用薬液５ａ＋１をゼータ電位フィルターに通過
させる。(2) Prepare a test drug solution (100ug/ml),
The Kono test chemical solution 5a+1 is passed through a zeta potential filter.
（３） （２）のゼータ電位フィルターを（１〉の発育
ブロスに入れ、３７°Ｃで培養する。また、菌液のみを
接種した発育ブロスも同時に３７°Ｃで培養し、これを
コントロールとする。(3) Place the zeta potential filter from (2) into the growth broth from (1) and culture at 37°C.Also, culture the growth broth inoculated with only the bacterial solution at 37°C at the same time, and use this as a control. do.
（４）コントロール、ゼータ電位フィルターを入れた発
育ブロス中の菌量を測定する。(4) Measure the amount of bacteria in the growth broth containing the control and zeta potential filter.
（試験結果）
上記濃度のＡＢＰＣ，ＣＥＴＳＣＭを通過させたゼータ
電位フィルターは、コントロールと同様の発育を示し、
発育プロス中の菌の発育には影響を与えなかった。(Test results) The zeta potential filter through which ABPC and CETSCM of the above concentration were passed showed the same growth as the control.
It did not affect the growth of bacteria during the growth process.
次に、この出願の発明の血中微生物培養方法に用いる培
養器の具体例について説明する。Next, a specific example of an incubator used in the blood microorganism culturing method of the invention of this application will be described.
図は、その培養器の一例を示しており、培地（１）とし
ての発育ブロスの入った培養ボトル（２）に、ゼータ電
位フィルター（３）を備えた蓋体（４）を捩じ込んで構
成している。The figure shows an example of such an incubator, in which a lid (4) equipped with a zeta potential filter (3) is screwed into a culture bottle (2) containing growth broth as a medium (1). It consists of
前記蓋体（４）は、中央に血液注入路（５）を有してお
り、前記ゼータ電位フィルター（３）がこの蓋体（４）
の側壁（６）に支持され、血液注入路（５）に配されて
いる。このゼータ電位フィルター（３）の周囲には、蓋
体（４）の側壁（６）内部に設けられた吸水体（７）を
接触させている。The lid (4) has a blood injection path (5) in the center, and the zeta potential filter (3) is connected to the lid (4).
is supported on the side wall (6) of the blood injection channel (5). A water absorbent body (7) provided inside the side wall (6) of the lid (4) is brought into contact with the periphery of the zeta potential filter (3).
また、血液注入路（５）には、前記ゼータ電位フィルタ
ー（３）の中央部を切り落とす切刃（８）を先端に備え
た筒体（９〉を上下動可能に捩じ込んでいる。この筒体
（９）には、密閉蓋（１０）を嵌め込んでいる。さらに
、前記ゼータ電位フィルター（３）の下面は、非吸水体
（１１）により覆われている。Further, a cylinder body (9>) equipped with a cutting blade (8) at the tip for cutting off the central part of the zeta potential filter (3) is screwed into the blood injection path (5) so as to be movable up and down. A sealing lid (10) is fitted into the cylindrical body (9).Furthermore, the lower surface of the zeta potential filter (3) is covered with a non-water absorbing material (11).
なお、前記蓋体（４）は、ゼータ電位フィルター（３）
の上下で分離できるようにした二分割体として構成して
おり、ゼータ電位フィルター（３）、及びこのゼータ電
位フィルター（３）の周囲に設けた吸水体（７）を交換
できるようにしている。Note that the lid (4) is a zeta potential filter (3).
The zeta potential filter (3) and the water absorbing body (7) provided around the zeta potential filter (3) can be replaced.
図は、この出願の発明の血中微生物培養方法に用いる培
養器の断面図。
（１）・・・培地
（３）・・・ゼータ電位フィルターThe figure is a sectional view of an incubator used in the blood microorganism culturing method of the invention of this application. (1)...Medium (3)...Zeta potential filter
Claims (1)
に吸着させると共に、ゼータ電位フィルターから薬剤及
び血液成分を除去したのち、ゼータ電位フィルターに吸
着させた微生物を培養することを特徴とする血中微生物
培養方法。 ２、ゼータ電位フィルターに吸水体を接触させることに
より、ゼータ電位フィルターから薬剤及び血液成分を除
去することを特徴とする請求項１記載の血中微生物培養
方法。 ３、吸水体を一部に接触させたゼータ電位フィルターを
備えると共に、このゼータ電位フィルターの吸水体を接
触させていない部分を切り落とす切刃を備え、さらにこ
のゼータ電位フィルターの下方に培地を備えたことを特
徴とする血中微生物培養方法に用いる培養器。 ４、ゼータ電位フィルターの下面を非吸水体で覆ったこ
とを特徴とする請求項３記載の血中微生物培養方法に用
いる培養器。[Scope of Claims] 1. Adsorbing microorganisms in blood containing drugs to a zeta potential filter, removing drugs and blood components from the zeta potential filter, and then culturing the microorganisms adsorbed to the zeta potential filter. Characteristic method for culturing blood microorganisms. 2. The method for culturing blood microorganisms according to claim 1, characterized in that drugs and blood components are removed from the zeta potential filter by bringing a water absorbent into contact with the zeta potential filter. 3. Equipped with a zeta potential filter that partially contacts the water absorbing material, a cutting blade that cuts off a portion of the zeta potential filter that is not in contact with the water absorbing material, and further provided with a culture medium below the zeta potential filter. An incubator used in a method for culturing blood microorganisms, characterized by: 4. The incubator used in the method for culturing blood microorganisms according to claim 3, wherein the lower surface of the zeta potential filter is covered with a non-water absorbing material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP34076789A JP2866973B2 (en) | 1989-12-28 | 1989-12-28 | Blood microbial culture method and incubator used for the method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP34076789A JP2866973B2 (en) | 1989-12-28 | 1989-12-28 | Blood microbial culture method and incubator used for the method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03201977A true JPH03201977A (en) | 1991-09-03 |
| JP2866973B2 JP2866973B2 (en) | 1999-03-08 |
Family
ID=18340116
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP34076789A Expired - Fee Related JP2866973B2 (en) | 1989-12-28 | 1989-12-28 | Blood microbial culture method and incubator used for the method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2866973B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001005995A1 (en) * | 1999-07-15 | 2001-01-25 | Mitsui Chemicals, Inc. | Method of preparing cell cultivation supernatant |
| JP2012198169A (en) * | 2011-03-23 | 2012-10-18 | Mitsui Eng & Shipbuild Co Ltd | Fluorescence detection device and fluorescence detection method |
-
1989
- 1989-12-28 JP JP34076789A patent/JP2866973B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001005995A1 (en) * | 1999-07-15 | 2001-01-25 | Mitsui Chemicals, Inc. | Method of preparing cell cultivation supernatant |
| JP2012198169A (en) * | 2011-03-23 | 2012-10-18 | Mitsui Eng & Shipbuild Co Ltd | Fluorescence detection device and fluorescence detection method |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2866973B2 (en) | 1999-03-08 |
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