JPH03165826A - Method of agitating solution - Google Patents

Abstract

PURPOSE:To agitate small amount of solution by receiving small amount of solution in a sample bottle, setting the sample bottle in a cylindrical member so that the side wall is attached to the outer wall of the bottom of the cylindrical member, and rotating the cylindrical member on a concave saucer. CONSTITUTION:A swinging means 2, the saucer 3, the cylindrical member 4 and the sample bottle 5 constitute a shaking device 1. The saucer 3 is made to have a concave curved surface having a same cross-sectional shape in the longitudinal direction to which the swinging mean 2 is fixed so as to be swung in the longitudinal direction, and the sample bottle 5 is detachably set on the bottom outer wall of the cylindrical member 4. Liquid 6 is received in the cylindrical member 4 so that a space is remained, and the cylindrical member is hermetically plugged, and the solution 7 to be agitated is received in the sample bottle 5 so that a space is also remained. The cylindrical member 4 is laid on the saucer 3, and, when the saucer is swung in the longitudinal direction by the swinging means 2, the cylindrical member 4 is rotated on the saucer 3. Thus, the small amount of solution is gently agitated for a long period.

Description

[Detailed Description of the Invention] [Summary] Regarding a method for stirring a solution, the purpose is to efficiently stir a small amount of solution in particular, and a shaking device and a shaking device including a shaking means, a saucer, a cylindrical member, and a sample bottle are provided. None, the tray has a concave curved surface with an equal cross-sectional shape in the longitudinal direction, and is fixed to a swinging means so as to swing in the longitudinal direction, and the side wall of the sample bottle is at the bottom of the cylindrical member. The cylindrical member is removably held on the outer wall, and then a liquid is poured into the cylindrical member so as to leave a gap and the sample bottle is sealed tightly, and then a solution to be stirred is poured into the sample bottle so as to leave a gap. The cylindrical member is sealed tightly, and then placed on a receiving tray, and then when the receiving tray is swung in the longitudinal direction by the swinging means, the cylindrical member is configured to roll on the concave curved surface of the receiving tray. .

[Industrial application field]

The present invention relates to a method for stirring a solution, and particularly to a method for gently and efficiently stirring a small amount of solution over a long period of time.

In recent years, with the advancement of science and technology, we are increasingly handling very expensive substances such as bioorganic materials, which are difficult to obtain in large quantities or do not exist naturally.

These substances cannot be handled in large amounts, such as hot water, and the amount used in various experiments is, for example, 0.5 at most.
It has to be in very small quantities such as yd.

Generally, such substances are often treated as a solution by dissolving them in an appropriate solvent.

How to efficiently stir such a small amount of solution has become an important technique, and an effective method is required.

[Conventional technology]

Whether it's creating a solution by dissolving a very small amount of a substance in a small amount of solvent, or stirring a small amount of solution to cause a chemical reaction, stirring techniques for small amounts of solutions are important in handling solutions. This is an important technology.

The stirring method depends on the conditions such as the amount of solute and solvent to be treated, the amount of solution and the size of the corresponding container, for example, whether it is an aqueous solution or an organic solvent solution, and if a chemical reaction is to be carried out, the temperature and viscosity of the solution. , various stirring devices are used.

Commonly known stirring devices include a magnetic stirrer (trademark) that rotates a magnetic piece inside a container by the guidance of a rotating magnet, a propeller-type stirring device, a shaking device such as a shaker, and a shaking device such as a shaker that rotates a magnetic piece inside the container by the guidance of a rotating magnet. There is a Vortech mixer (trademark) that shakes the bottom of the container by touching it.

These various stirring devices are either suitable for relatively large containers, such as 50 ml or more, or are not suitable for stirring a small amount of solution that is less than ITL.

On the other hand, in analyses, such as clinical tests and environmental analyses, or in tests and experiments that use special reagents such as antibodies or enzymes, gentle stirring is desired, and expensive reagents are used. Since Case 4 is common, the amount of samples handled is generally very small, for example, several dozen.

A vortex mixer, in which a container is vibrated by hand, can be easily applied to stir small amounts of such solutions, but it is not suitable for long-term continuous use, and has disadvantages such as not being able to perform gentle stirring. There is.

In addition, a rotating roller type stirring device, for example, 10 ml'
Although it can be applied to a few containers, it is relatively expensive and ineffective for a few containers.

By the way, speaking of whether it is possible or not, a top-pan type shaking device can handle even very small containers.

FIG. 6 is a diagram showing a stirring method using a conventional shaking device.

5 This shaking device 1 is connected to a swinging means 2 and is provided with a tray 3, on which a sample bottle 5 containing a solution 7 to be stirred is appropriately held. The advantage of this shaking device 1 is that any small container can be placed on the tray 3 as long as it is held properly.

The tray 3 is configured to swing in the horizontal direction by an electric swinging means 2 using, for example, a rotary cam. As a result, the sample bottle 5 held on the tray 3 is shaken, and the agitated solution 7 in the sample bottle 5 is shaken.

The receiving tray 3 is designed to be able to vary the amplitude and period of its oscillation, so that optimal conditions can be set depending on the size of the sample bottle 5 and the type of the solution 7 to be stirred.

[Invention or problem to be solved]

The conventional method of holding a container containing a solution on a support tray of a shaking device and stirring without shaking involves holding a small container on the support tray.

6 However, the advantage of a rocking type shaking device is that it stirs the solution contained in a large container by shaking it.

Therefore, as the container becomes smaller, the amount of solution stored therein decreases, and the space for the solution to play within the container decreases, causing the problem that the efficiency of stirring the solution becomes extremely poor. .

However, if the repetition period is shortened by increasing the rocking speed, only the container will move due to the inertia of the solution and the solution will not move, so this will not improve the stirring efficiency.

Furthermore, if you want to stir the solution gently without giving it a sudden shock, for example because you don't want the solution to bubble, you need to slow down the rocking speed and lengthen the repetition period.
There was a problem in that the solution was hardly stirred.

The present invention adds simple accessories to the conventional shaking device, and
The purpose is to provide a method that allows a small amount of solution to be stirred gently and efficiently over a long period of time.

The problem described above is that the shaking device is made up of a swinging means, a receiving tray, a cylindrical member, and a sample bottle, and the receiving tray has a concave curved surface with an equal cross-sectional shape in the longitudinal direction, and that the receiving tray swings in the longitudinal direction. The sample bottle is fixed to the swinging means as shown in FIG. Fill the sample bottle with a liquid and seal it tightly. Next, pour the solution to be stirred into the sample bottle and seal it tightly, leaving a gap in the sample bottle. Next, place the cylindrical member on a receiving tray. This is achieved by a method of stirring the solution, in which the cylindrical member rolls on the concave curved surface of the saucer as it swings in the longitudinal direction.

[For production]

Various types of stirring methods have traditionally been used to stir solutions that have a certain amount of liquid.
While there was no stirring method suitable for efficiently stirring a small amount of solution, the stirring method of the present invention allows for efficient stirring of a small amount of solution.

That is, in the present invention, a small amount of the solution to be stirred is placed in a sample bottle, the sample bottle is attached so that its side wall sticks to the bottom outer wall of a cylindrical member, and the cylindrical member is attached to a concave curved surface that swings. It is rolled on a shaped tray.

FIG. 4 is a diagram showing the rotation of the sample bottle, and FIG. 5 is a diagram showing the revolution of the sample bottle.

First, in FIG. 4, the sample bottle 5 is
It rotates around the center. Due to the rotation of the sample bottle 5, the agitated solution 7 is stirred while falling back and forth from the top to the bottom inside the sample bottle.

However, considering the purpose of the present invention, which is to stir a small amount of the solution 7 to be stirred 9 - , the size of the sample bottle 5 is small, and its length is only a few degrees at most.

Therefore, this method of stirring using only the rotation of the sample bottle 5 is not efficient, and the solution to be stirred 7 inside cannot be sufficiently stirred.

Next, in FIG. 5, the sample bottle 5 is held by the outer wall of the bottom of the cylindrical member 4 and is rotated.

This revolving motion is caused by the cylindrical member 4 rolling back and forth like a pendulum while rolling along the concave curved surface of the receiving tray 3.
The locus of movement of the cylindrical member 4 will be drawn.

Moreover, this cylindrical member 4 has liquid 6, such as water, for example.
Put an appropriate amount of water in it, get up and do it like a small priest, so that it rolls stably on the saucer 3 without shaking.

Now, let us consider a case in which when the agitated solution 7 falls downward due to the rotational movement of the sample bottle 5, the cylindrical member 4 rotates upward while rolling on the concave curved surface of the receiving tray 3.

-10 At this time, the stirred solution 7 is about to hit the bottom of the sample bottle 5 downward, while the sample bottle 5 is about to be lifted upward. As a result, the stirred solution 7 hits the bottom of the sample bottle 5 without being subjected to a stronger impact force.

In this way, according to the present invention, compared to the conventional movement in which the stirred solution 7 simply moves back and forth in the sample bottle 5, the stirred solution 7, for example, which does not like vigorous stirring due to foaming, can be easily moved. However, a relatively slow rocking motion allows for more efficient stirring motion.

〔Example〕

FIG. 1 is a perspective view showing an embodiment of the present invention, FIG. 2 is a side view of FIG. 1 during operation, and FIG. 3 is a partially enlarged perspective view of a cylindrical member.

In the figure, l is a shaking device, 2 is a shaking means, 3 is a saucer, 4 is a cylindrical member, 5 is a sample bottle, 6 is a liquid, and 7 is a solution to be stirred.

The shaking device 1 is composed of a mechanism in which a tray 3 is fixed to a swinging means 2, which is an electric shaking mechanism using a cam, for example, a cylindrical member 4, and a sample bottle 5.

It is preferable that the swinging means 2 has a structure in which the amplitude of swinging can be varied by about 50 mm and the period can be varied up to about 3 times per second. This swinging means 2 includes, for example, MARYSOL
A rotary shaker manufactured by the company is suitable.

For example, the length of the receiving tray 3 in the longitudinal direction of swinging is 300 m.
It is made of an Al plate with a depth of 200 mm and has a concave curved shape that rises toward both longitudinal ends with smooth curved surfaces that look like something a cylindrical object could roll over. Then, to prevent the cylindrical member 4 rolling on the concave curved surface from slipping,
For example, it may be covered with rubber.

The cylindrical member 4 may be, for example, a polyethylene bottle, and may have various sizes, for example, an outer diameter of 60 mm.
It is a cylindrical container with a diameter of 100 mm and a capacity of 250 mm.

One end of the cylindrical member 4 has a bottom and is liquid-tight 1 2 , and a support 42 is provided on the outer wall 41 of the bottom so that the sample bottle 5 can be held therein.

This supporter 42 may be simply attached using adhesive tape, for example, but in order to stably hold the sample bottle 5 for a long time or multiple times, for example, a band that holds the sample bottle 5 is provided, and the band is engaged. It's good to be prepared to stop.

Further, a lid 43 is attached to the other end of the cylindrical member 4, so that an appropriate amount of liquid 6, such as water, can be put into the cylindrical member 4 and the lid 43 can be sealed tightly.

The sample bottle 5 only needs to have a length smaller than the diameter of the cylindrical member 4, and can be appropriately selected depending on the amount of the solution 7 to be stirred, or a container called a mini centrifuge tube made of polypropylene manufactured by EPPENDORF is suitable.

This mini centrifuge tube has an outer diameter of 10 mmφ and a length of 30 mm.
mm, and one end is open so that it can be sealed tightly.

Using the shaking device 1 configured in this way, the solution 13 is The results of a stirring experiment are described below.

Example; 1 A stirred solution 7 consisting of 0.2 g of sodium chloride is placed in a sample bottle 5 consisting of 21 mini-centrifuge tubes used as the sample bottle 5, and lmj of deionized water is gently added.

This sample bottle 5 is held by the cylindrical member 4 using a support 42 . Inside the cylindrical member 4, about 70-
It is filled with water and sealed tightly.

The amplitude of the swinging means 2 is set to 50 mm, and the cylindrical member 4 is placed on the container 3 and stirred while changing the repetition period, that is, the swinging speed.

When the stirring method according to the present invention is compared with a method using a conventional shaking device, the results are shown in Table 1.

Table 1 l Sodium chloride dissolution time (seconds) l 4 As can be seen from this Table 1, according to the conventional method, 4
At a rocking speed of 6 times/minute, it will not dissolve even if it takes more than 1 hour (h), but according to the method of the present invention, only 40
It can be seen that it can be dissolved in seconds and has good stirring efficiency.

Example=2 0. 5rId! A sample bottle 5 consisting of a mini centrifuge tube containing 2 rnl containing a stirred solution 7 consisting of 1 rnl bovine serum albumin aqueous solution containing agar of
Shake gently all day and night at the same rocking speed of 46 times/min.

After completion, it can be seen that the agar in the sample bottle 5 has not precipitated, indicating that sufficient stirring has been carried out, and that the stirred solution 7 has not bubbled, indicating that stirring has been carried out gently. .

The stirring conditions mentioned above, such as the rocking speed and amplitude, can be varied in various ways depending on the shape and size of the cylindrical member and sample bottle.

In addition, various modifications can be made to the formation of the concave curved surface of the saucer and the amount of liquid put into the cylindrical member for smooth rotation.

〔Effect of the invention〕

In contrast to the conventional stirring method using a shaking device in which it was difficult to stir a small amount of solution gently for a long time, the stirring method of the present invention has the following advantages:
A simple method that adds a simple mechanism to a conventional shaking device allows for gentle stirring over a long period of time.

Therefore, the present invention contributes to fields such as bioorganic chemistry, which are expected to further advance in the future, such as clinical tests where only a small amount of specimen is used and experiments performed using small amounts of expensive reagents. It's quite large.

[Brief explanation of the drawing]

Fig. 1 is a perspective view showing an embodiment of the present invention, Fig. 2 is a side view of Fig. 1 during operation, Fig. 3 is a perspective view of the cylindrical member, and Fig. 4 is a diagram showing the rotation of the sample bottle. , l 6 Figure 5 is a diagram showing the revolution of a sample bottle, and Figure 6 is a diagram showing a stirring method using a conventional shaking device. In the figure, 1 is a shaking device, 3 is a saucer, 5 is a sample bottle, and 7 is a stirred solution. 2 is a swinging means, 4 is a cylindrical member, 6 is a liquid, jA1 7

Claims (1)

[Claims] A shaking device (1) is constituted by a swinging means (2), a tray (3), a cylindrical member (4), and a sample bottle (5), and the tray (3) is arranged in a longitudinal direction. The rocking means (2) has a concave curved surface having an equal cross-sectional shape and swings in the longitudinal direction.
The sample bottle (5) has its side wall removably held by the outer wall of the bottom of the cylindrical member (4), and then the sample bottle (5) is attached to the cylindrical member (4). Pour the liquid (6) into the sample bottle (5) and seal it tightly so that a void remains, then pour the solution to be stirred (7) into the sample bottle (5) and seal it tightly so that a void remains. ) is placed on the tray (3), and then the tray (3) is moved by the swinging means (2).
A method for stirring a solution, characterized in that when the cylindrical member (4) is swung in the longitudinal direction, the cylindrical member (4) rolls on the concave curved surface of the receiving tray (3).