JPH03144357A - Liquid separator for lc/ms interface - Google Patents
Liquid separator for lc/ms interfaceInfo
- Publication number
- JPH03144357A JPH03144357A JP1284392A JP28439289A JPH03144357A JP H03144357 A JPH03144357 A JP H03144357A JP 1284392 A JP1284392 A JP 1284392A JP 28439289 A JP28439289 A JP 28439289A JP H03144357 A JPH03144357 A JP H03144357A
- Authority
- JP
- Japan
- Prior art keywords
- liquid
- interface
- vacuum pump
- liquid sample
- atomized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 27
- 238000000926 separation method Methods 0.000 claims abstract description 16
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 claims abstract description 11
- 238000000889 atomisation Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 abstract description 6
- 239000007789 gas Substances 0.000 description 4
- 239000001307 helium Substances 0.000 description 4
- 229910052734 helium Inorganic materials 0.000 description 4
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000003209 petroleum derivative Substances 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B17/00—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups
- B05B17/04—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods
- B05B17/06—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations
- B05B17/0607—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers
- B05B17/0623—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers coupled with a vibrating horn
- B05B17/063—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers coupled with a vibrating horn having an internal channel for supplying the liquid or other fluent material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
- B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
- B05B17/00—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups
- B05B17/04—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods
- B05B17/06—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations
- B05B17/0607—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers
- B05B17/0623—Apparatus for spraying or atomising liquids or other fluent materials, not covered by the preceding groups operating with special methods using ultrasonic or other kinds of vibrations generated by electrical means, e.g. piezoelectric transducers coupled with a vibrating horn
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Special Spraying Apparatus (AREA)
- Vaporization, Distillation, Condensation, Sublimation, And Cold Traps (AREA)
- Electron Tubes For Measurement (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、液体クロマトグラフ装置（ＬＣ）と質量分析
装置（ＭＳ）との間に接続されるＬＣ／ＭＳインターフ
ェース用液体分離装置に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a liquid separation device for an LC/MS interface connected between a liquid chromatograph (LC) and a mass spectrometer (MS).
液体クロマトグラフ装置（ＬＣ）・は、液体溶液の成分
分離に優れた威力を有するが、成分の構造に関する情報
は得られず、これと質量分析装置（ＭＳ）を組み合わせ
て、ＬＣで分離した各留分をＭＳで分析するＬＣ／ＭＳ
分析法は、極めて僅かの試料で行えるので、石油製品に
含まれる複雑な炭化水素類、蛋白質の加水分解物等の分
析に欠かせぬ手段となっている。この、ＬＣ／ＭＳ分析
法においては、ＬＣのカラムから出てくる液体試料をＭ
Ｓの高真空のイオン化室に導入しなければならないので
、液体試料から溶媒を除去するための分離装置が必要で
ある。Although liquid chromatography (LC) has excellent ability to separate components of liquid solutions, it cannot obtain information about the structure of the components. LC/MS to analyze fractions by MS
Since the analytical method can be performed with an extremely small amount of sample, it has become an indispensable means for analyzing complex hydrocarbons, protein hydrolysates, etc. contained in petroleum products. In this LC/MS analysis method, the liquid sample coming out of the LC column is
A separation device is required to remove the solvent from the liquid sample since it must be introduced into the high vacuum ionization chamber of S.
従来、この分離装置としては、液体試料をヘリウムガス
を用いてノズルから噴霧させ、霧化された液滴をヘリウ
ムガスにより搬送させる過程で蒸発成分を蒸発、分離さ
せ、重質留分をＭＳに導入している。Conventionally, this separation device atomizes a liquid sample from a nozzle using helium gas, evaporates and separates the evaporated components in the process of transporting the atomized droplets with helium gas, and converts the heavy fraction into an MS. It has been introduced.
［発明が解決しようとする課題］
しかしながら、上記従来の分離装置においては、液体試
料を霧化、搬送するために多量のヘリウムガスを用いる
ため、装置が大型化すると共に霧化効率が悪（、また、
分離される不蒸発成分が希釈されるため、ＭＳにおける
測定感度が低下するという問題を有している。[Problems to be Solved by the Invention] However, in the conventional separation device described above, a large amount of helium gas is used to atomize and transport the liquid sample, resulting in an increase in the size of the device and poor atomization efficiency ( Also,
Since the non-evaporable components to be separated are diluted, there is a problem in that the measurement sensitivity in MS is reduced.
本発明は、上記問題を解決するものであって、コンパク
トでかつ分離能力の高いＬＣ／ＭＳインターフェース用
液体分離装置を提供することを目的とする。The present invention solves the above problems, and aims to provide a liquid separation device for an LC/MS interface that is compact and has high separation ability.
そのために本発明のＬＣ／ＭＳインターフェース用液体
分離装置は、分離器本体１と、分離本体１の一端に固定
される超音波霧化装置２と、分離器本体１の他端に設け
られる試料供給管３と、濃縮器本体１に接続される真空
ポンプ４とからなり、超音波霧化装置２をＬＣに接続し
、試料供給管３をＭＳに接続することを特徴とする。To this end, the liquid separation device for LC/MS interface of the present invention includes a separator body 1, an ultrasonic atomizer 2 fixed to one end of the separation body 1, and a sample supply provided at the other end of the separator body 1. It consists of a tube 3 and a vacuum pump 4 connected to the concentrator main body 1, and is characterized in that the ultrasonic atomizer 2 is connected to the LC, and the sample supply tube 3 is connected to the MS.
なお、上記構成に付加した番号は、理解を容易にするた
めに図面と対比させるためのもので、これにより本発明
の構成が限定されるものではない。Note that the numbers added to the above configurations are for comparison with the drawings to facilitate understanding, and the configurations of the present invention are not limited thereby.
本発明においては、例えば、第１図に示すように、ＬＣ
のカラムを出た液体試料は、振動子ホーン２ｂの霧化部
２ｃに噴霧され、霧化部２Ｃにおいて超音波振動により
霧化され、霧化され表面積が増大した液滴は、真空ポン
プ４により開口１ｂに向かって搬送される過程で効率良
く蒸発され、開口１ｂから噴霧される。そして、蒸発成
分は、真空ポンプ４により吸引され、不蒸発成分である
重質留分は、試料供給管３を経てＭＳのイオン化室に導
入される。In the present invention, for example, as shown in FIG.
The liquid sample exiting the column is atomized to the atomization section 2c of the vibrator horn 2b, where it is atomized by ultrasonic vibration, and the atomized droplets with increased surface area are atomized by the vacuum pump 4. In the process of being conveyed toward the opening 1b, it is efficiently evaporated and sprayed from the opening 1b. Then, the evaporated components are sucked by the vacuum pump 4, and the heavy fraction, which is the non-evaporated components, is introduced into the ionization chamber of the MS via the sample supply tube 3.
以下本発明の実施例を図面を参照しつつ説明する。第１
図は本発明のＬ Ｃ／Ｍ Ｓインターフェース用液体分
離装置の一実施例を示す断面図である。Embodiments of the present invention will be described below with reference to the drawings. 1st
The figure is a sectional view showing an embodiment of the liquid separation device for LC/MS interface of the present invention.
図において本発明のＬＣ／ＭＳインターフェース用液体
分離装置は、分離器本体１と、分離本体１の一端に固定
される超音波霧化装置２と、分離器本体１の他端に設け
られる試料供給管３と、分離器本体１に接続される真空
ポンプ４とからなる。In the figure, the liquid separation device for LC/MS interface of the present invention includes a separator body 1, an ultrasonic atomizer 2 fixed to one end of the separation body 1, and a sample supply provided at the other end of the separator body 1. It consists of a pipe 3 and a vacuum pump 4 connected to the separator body 1.
超音波霧化装ｆ２は、基部に超音波振動を発生させるた
めの超音波振動発生部２ａを備え、この超音波振動発生
部２ａには、振動子ホーン２ｂが連結されており、この
振動子ホーン２ｂの先端部には、先端が中空の霧化部２
Ｃが形成され、また、振動子ホーン２ｂの内部には、液
体試料供給路２ｄが形成されている。なお、霧化部２Ｃ
の形状および液供給の方法は、上記実施例に限定される
ものではなく、従来周知の形状、方法を採用してもよい
。The ultrasonic atomizer f2 is equipped with an ultrasonic vibration generator 2a for generating ultrasonic vibrations at the base, and a vibrator horn 2b is connected to the ultrasonic vibration generator 2a. At the tip of 2b, there is an atomizing section 2 with a hollow tip.
A liquid sample supply path 2d is formed inside the vibrator horn 2b. In addition, the atomization part 2C
The shape and method of supplying liquid are not limited to those in the above embodiments, and conventionally known shapes and methods may be adopted.
分離器本体１の中央部には、開口１ｂを有するノズル体
１ａが形成されると共に、試料供給管３の近傍に排出管
１ｃが形成され、該排出管ＩＣが真空ポンプ４に接続さ
れている。そして、前記液体試料供給路２ｄがＬＣのカ
ラムに接続され、試料供給管３がＭＳのイオン化室に接
続されている。A nozzle body 1a having an opening 1b is formed in the center of the separator body 1, and a discharge pipe 1c is formed near the sample supply pipe 3, and the discharge pipe IC is connected to a vacuum pump 4. . The liquid sample supply path 2d is connected to the LC column, and the sample supply pipe 3 is connected to the ionization chamber of the MS.
その作用について説明すると、ＬＣのカラムを出た液体
試料は、矢印Ａから振動子ホーン２ｂの内部に形成され
た液体試料供給路２ｄを経て霧化部２ｃに噴霧され、霧
化部２ｃにおいて超音波振動により霧化される。To explain its operation, the liquid sample that has exited the LC column is atomized from the arrow A through the liquid sample supply path 2d formed inside the vibrator horn 2b to the atomizing section 2c, where it is superimposed. Atomized by sonic vibration.
霧化部２Ｃにおいて霧化され表面積が増大した液滴は、
真空ポンプ４により開口１ｂに向かって搬送される過程
で効率良く蒸発され、開口１ｂから噴霧される。そして
、蒸発成分は、真空ポンプ４により吸引され、不蒸発成
分である重質留分は、矢印Ｂで示すように試料供給管３
を経てＭＳのイオン化室に導入される。The droplets that are atomized in the atomization section 2C and have an increased surface area are
In the process of being conveyed toward the opening 1b by the vacuum pump 4, it is efficiently evaporated and sprayed from the opening 1b. The evaporated components are sucked by the vacuum pump 4, and the heavy fraction, which is the non-evaporated components, is transferred to the sample supply tube 3 as shown by arrow B.
The sample is introduced into the ionization chamber of the MS.
なお、本発明においては、分離器本体の長さが１０ｃ■
程度にまで小型にすることができる。In addition, in the present invention, the length of the separator body is 10 cm
It can be downsized to a certain degree.
以上、説明したように本発明によれば、従来のように液
体試料を霧化、搬送するために多量のヘリウムガスを用
いず、超音波霧化装置のみにより霧化するため、コンパ
クトでかつ分離能力の高いＬＣ／ＭＳインターフェース
用液体分離装置を提供することができる。As explained above, according to the present invention, a large amount of helium gas is not used to atomize and transport a liquid sample as in the past, and atomization is performed only by an ultrasonic atomizer, so that the liquid sample can be compact and separated. A liquid separation device for an LC/MS interface with high performance can be provided.
【図面の簡単な説明】
第１図は本発明のＬＣ／ＭＳインターフェース用液体分
離装置の一実施例を示す断面図である。
１・・・分離器本体、２・・・超音波霧化装置、４・・
・真空ポンプ。BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a sectional view showing an embodiment of the liquid separation device for LC/MS interface of the present invention. 1... Separator main body, 2... Ultrasonic atomization device, 4...
·Vacuum pump.
Claims (1)
音波霧化装置と、分離器本体の他端に設けられる試料供
給管と、濃縮器本体に接続される真空ポンプとからなり
、前記超音波霧化装置をＬＣに接続し、前記試料供給管
をＭＳに接続することを特徴とするＬＣ／ＭＳインター
フェース用液体分離装置。(1) Consists of a separator body, an ultrasonic atomizer fixed to one end of the separation body 1, a sample supply pipe provided at the other end of the separator body, and a vacuum pump connected to the concentrator body. , a liquid separation device for an LC/MS interface, characterized in that the ultrasonic atomization device is connected to an LC, and the sample supply tube is connected to an MS.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1284392A JPH03144357A (en) | 1989-10-30 | 1989-10-30 | Liquid separator for lc/ms interface |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1284392A JPH03144357A (en) | 1989-10-30 | 1989-10-30 | Liquid separator for lc/ms interface |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03144357A true JPH03144357A (en) | 1991-06-19 |
Family
ID=17677983
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1284392A Pending JPH03144357A (en) | 1989-10-30 | 1989-10-30 | Liquid separator for lc/ms interface |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03144357A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103239877A (en) * | 2013-05-27 | 2013-08-14 | 上海兴全电力技术有限公司 | High-efficiency hot air source evaporator |
| CN109839421A (en) * | 2017-11-27 | 2019-06-04 | 中国科学院大连化学物理研究所 | The method quickly detected for the direct mass spectrography of semi-volatile organic matter in liquid |
-
1989
- 1989-10-30 JP JP1284392A patent/JPH03144357A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103239877A (en) * | 2013-05-27 | 2013-08-14 | 上海兴全电力技术有限公司 | High-efficiency hot air source evaporator |
| CN109839421A (en) * | 2017-11-27 | 2019-06-04 | 中国科学院大连化学物理研究所 | The method quickly detected for the direct mass spectrography of semi-volatile organic matter in liquid |
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