JPH03136649A - Mammitis preventing antibacterial cloth for dairy cow - Google Patents

Abstract

PURPOSE:To economically and safely prevent the diffusion of mammitis by using a mammitis preventing antibacterial cloth for dairy cow containing antibacterial metal atoms. CONSTITUTION:The materials for an antibacterial cloth include natural fibers such as cotton and rayon as well as synthetic fibers such as polyacrylonitrile, polyester and nylon. Metal atoms supported by these materials include silver, copper, zinc and iron, and the metal atoms may be used independently or in combination of several kinds thereof. The amount of the metal atoms contained in the antibacterial cloth is preferably 1 ppm-10 wt.% of the antibacterial cloth, and particularly, 50 ppm-1 wt.%. In the manufacture of these materials, for example, silver-containing polyacrylonitrile can be obtained by dissolving a water-soluble silver compound in water, adding a dyeable polyacrylonitrile thereto, and treating the resulting mixture at a temperature of glass transition temperature or more followed by washing and drying.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to an antibacterial cloth having a mastitis preventive effect and used for cleaning the udders and teats of milking cows.

(Conventional technology) When milking a milking cow, in order to remove and clean the udder and teats of manure, soil, dirt, germs, etc., the cows are washed with hot or cold water, and then towels and sterilizers are moistened. Wipe with towels, disinfectant-coated paper towels, disposable paper, etc.

[Problem to be solved by the invention]

When wiping the breasts and nipples with conventional towels,
Staphylococcus, streptococcus,
Corynebacterium, Escherichia coli, etc. spread to other healthy breasts through towels, causing mastitis. In addition, when wiping off disinfectant etc. with a damp towel,
It was necessary to prepare an aqueous solution containing a disinfectant in advance, and the labor involved in wiping the disinfectant off the breasts was a problem.

In addition, there is a limit to the amount of disinfectant, disinfectant, etc. that can be applied to paper towels with disinfectants.
There is a problem that there is a limit to the number of dairy cows that can be cleaned with a sheet of paper, and that running costs are too high, including disposable paper. In addition, disinfectant,
If even a small amount of disinfectant remained, there was a risk that it would migrate into the milk.

[Means to solve the problem]

The present inventors have completed the present invention as a result of intensive studies to solve the above problems.The present invention relates to an antibacterial cloth for preventing mastitis in milking cows containing anti-concave metal atoms. Wipe the udder and teats of the milking cow using a cloth,
When the udder and teat are cleaned, it has the same mastitis prevention effect as a cloth moistened with a contagious agent, and the product of the present invention has an extremely low risk of releasing substances harmful to the human body. In addition, the antibacterial cloth of the present invention can prevent the occurrence of mastitis in milking cows better than other ordinary towels, paper towels, etc., is easy to use, and is effective in preventing the spread of mastitis. It is much more economical than disposable paper towels, which are considered effective in many areas.

Note that the term "cloth" as used in the present invention refers to not only woven fabrics and non-woven fabrics, but also
Indicates textile products including knitted fabrics.

Materials for the antibacterial cloth of the present invention include synthetic fibers such as polyacrylonitrile, polyester, and nylon, as well as natural fibers such as cotton and rayon. In addition to cloths manufactured using these materials alone, it is also possible to use cloths manufactured by blending or interweaving. Examples of the metal atoms supported on these materials include silver, copper, zinc, and iron, and the metal atoms may be used alone or in combination with several elements.

Furthermore, in addition to being bonded to the material as ions, these atoms can also be supported on the material as ultrafine metal particles. The above-mentioned fiber materials can be used in the same manner even if they have pores or voids on their surface or are so-called water-absorbing fibers composed of ultra-fine fibers.

The amount of metal atoms contained in the antibacterial cloth is preferably 1 ppm to 10% by weight, particularly 50 ppm to 10% by weight of the antibacterial cloth.
1% by weight is preferred.

The antibacterial cloth of the present invention can be produced by various methods.6 For example, to obtain silver-containing polyacrylonitrile, a water-soluble silver compound is dissolved in water, dyeable polyacrylonitrile is added, and the glass transition temperature is increased. After processing at the above temperature, it can be obtained by washing with water and drying. Such treatment can be carried out in the fiber state, but it can also be carried out after the fibers are woven into a cloth. If the cloth is treated in the form of a cloth, it can be left as is, or if it is treated in the form of fibers, it can be refrothed by a normal method to make the mastitis prevention antibacterial cloth of the present invention. can.

Mastitis in Japan always ranks first among fatal accidents by disease name, accounting for around 26% of all cases.

On the other hand, according to a survey conducted by livestock health centers nationwide regarding subclinical mastitis, the average number of cows was 46.6% (24-52%) and the number of quarters was 21.9% (8-41%) )19
The incidence rate is high (80), making it a fairly serious problem.

Mastitis is a direct cause of microbial infection or various physical and chemical sensitizations caused by an unspecified number of bacteria, viruses, etc. that are distributed on the body surface or in the environment. Inflammation of the breast occurs as a result of a combination of indirect predisposing or triggering environmental conditions.

There are a wide variety of causes for mastitis, including genetics, constitution, season or climate, barn structure, environment, number of animals kept, feed, milking method, lactation period, diseases other than the udder, relationship with hormonal status, immunodeficiency, treatment, etc. It is difficult to identify the mechanism of action for the onset of mastitis, as these factors are intricately involved with microbial infections such as bacteria and viruses as direct causes.

Currently, dairy farmers use towels moistened with disinfectants or paper towels coated with disinfectants in order to prevent infections caused by bacteria, viruses, etc., which are the direct cause of infection. The penetration rate is very low.

Currently, the methods of milking in Japan are as follows: 1. Dip and wash a towel or tenugui in warm water alone or in warm water containing a disinfectant, and then wash the breasts and teats by masturbating them.

■ Squeeze the milk (residual milk in the nipple) ■ Breedip the nipple with disinfectant and wipe it with a paper towel or towel.

■ Operate the milker and express milk.

■ After milking, disinfect the teats with disinfectant. (dipping), and the product of the present invention (antibacterial cloth) can be used in the steps ① and ②.

The product of the present invention can be used alone for the purpose of wiping and cleaning after cleaning the breast and nipple.

In addition, sterilization and disinfectants (■ diluted solutions of disinfectants containing henzalkonium chloride, [mono, bis (trimethylammonium methylene chloride)] alkyl (C9-Cl5) toluene as inverse soap preparations, ■ polyamide as amphoteric soap preparations) Even better results can be obtained by using a dilute solution of a disinfectant containing octylaminoethylglycine, polyoxyethylene alkylphenol ether, etc.) to moisten the above-mentioned product of the present invention.

[Example] Next, examples will show that the antibacterial cloth according to the present invention has excellent effects.

[Preparation Example 1] Production of antibacterial cloth Distilled water of No. 61 was placed in an enamel container No. 101 and heated to 95°C. 100 mg of silver nitrate was added to this and dissolved with stirring to obtain an aqueous silver nitrate solution. 200 g of a cloth woven from dyeable acrylic fibers was soaked in water in advance and then added to the silver nitrate aqueous solution. The mixture was heated and stirred slowly while being careful not to lift the cloth, and stirring was continued for 30 minutes after the bath temperature reached 95°C. As a result of stopping heating and slowly cooling without stirring, the bath temperature reached 40° C. after 3 hours. Remove the cloth from the bath and add 10 liters of water! After washing three times with water and drying to obtain an antibacterial cloth, the silver support device of 0 cloth was analyzed by atomic absorption spectrometry and found to be 302 ppm.

The following tests were conducted using the cloth made in this production example (product of the present invention).
This was done using

Example 1 1. Test bacteria ■ Streptococcus agal
actice) ■ Staphylococcus
cus aureus) ■Corynebacterium (Corynebacterium + pyogene)
s) ■ Escherichia coli (E, coli) ■ Pseudomonas aerugi
nosa)■Others (Clostridium
perfringens) 2. Test method: As mentioned above, using typical bacteria detected in milk from mastitis, the Textile Sanitary Processing Council's "Accreditation Standards for Antibacterial and Deodorant Processed Products" Attached Table 2 Processing Effect Test Method 2. A confirmation test was conducted using a modified shake flask method.

(1) Preparation of test bacteria suspension Perform main culture in each country using an appropriate culture solution, and adjust the culture solution using Nutrient Broth (Dirco) so that the number of bacteria per ld is approximately 10". rear,
Furthermore, 11! using phosphate buffer! The number of bacteria per unit is approximately 1O
This was diluted to give a test bacterial suspension.

(2) Test procedure Pour 70I11 of phosphate buffer into an Erlenmeyer flask (200 paper capacity with screw cap), and after sterilization, add 5 d of test bacteria suspension to use as test solution.0-order finely cut test sample. (
Add 1.5g of the antibacterial cloth of the present invention and store at 25℃ for 5 minutes.
A test solution that had been shaken for a minute (horizontal shaking at 100 rpm) and that did not contain any additives was treated in the same manner as a blank.

The test was conducted by preparing three specimens and three blanks, and the average values were compared.

(3) Measurement of the number of viable bacteria The number of viable bacteria in each test solution before shaking (before addition of the sample) and after shaking was determined using a plate culture method using a bacterial count measurement medium suitable for each bacterial species ( (incubation at 37°C for 148 hours).

3. The number of viable bacteria per test solution Ld before shaking (before the product of the present invention has been left to rest) and after shaking for 5 minutes. was not detected.

This suggests that cleaning the udder with the product of the present invention can prevent mastitis from spreading and contaminating it. In addition, ■ ClosL
For S. ridium perfringens, all test operations were performed under anoxic conditions.

Example 2 When milking a dairy cow in the usual manner, the udder was washed with warm water and then wiped clean with the antibacterial cloth of the present invention. Thereafter, the dirt on the product of the present invention was removed with warm water. This was repeated one by one for each animal, and at the end of the 30th animal, the number of E. coli bacteria adhering to the antibacterial cloth was measured within 5 minutes. The comparison sample was as follows, and the same operation as above was performed.

Comparison sample No. 1 Ordinary towel (ordinary commercially available towel) No. 2 Ordinary towel soiled with benzalkonium chloride diluted hot water No. 3 Benzalkonium chloride adhesion paper No.
4 (0 from the paper 1, used antibacterial cloth (product of the present invention) and comparison sample)
．． Cut out 2g, buffered saline 20+11! The adherent E. coli was separated by shaking.

The solution was spread on a DHL agar plate medium, and the number of E. coli bacteria per gram of the test material was measured.

2. Results: Escherichia coli was not detected in the towels moistened with disinfectant (hemazalkonium chloride) and in the product of the present invention.

This confirms that it is possible to prevent the spread of bacterial infection, which is the main cause of mastitis.

More than 10 pieces of E. coli were detected on the disposable paper No. 4, but since it is disposable, there is no need to worry about it spreading.

Since the disinfectant-attached paper of No. 3 has a wiping limit of 25 animals per sheet, more than 103,378 bacteria were detected in the 30th animal.

Example 3 Five farms with frequent mastitis were selected, and the number of cows with mastitis was compared with past data in order to see the effects of the product of the present invention. In the above five cases, the breasts were cleaned using only warm water using a regular towel, and the product of the present invention was used from the start of the test.

1. Method: The feeding management of each dairy farmer was left to the individual's own method, and the product of the present invention was used only for cleaning the udders and teats. At the start of the study, thorough treatment was performed using an antibacterial agent (nafcillin sodium monohydrate) during mastitis outbreaks.

2. Test period: January 1989 to June 1989 3. Results From the above results, it is clear that by using the product of the present invention,
The number decreased during mastitis disease, indicating that the product of the present invention is effective against the spread infection of mastitis.

Example 4 An antibacterial cloth was produced in the same manner as in Production Example 1 except that copper nitrate, ferrous sulfate, or zinc nitrate was used instead of silver nitrate. When tests similar to those in Example 1 were conducted using these cloths, similar effects were obtained in all cases.

〔Effect of the invention〕

By using the antibacterial cloth of the present invention, it is possible to prevent the occurrence of mastitis during milking.

Claims (1)

[Claims] 1. Antibacterial cloth for preventing mastitis in milking cows containing antibacterial metal atoms. 2. The antibacterial cloth according to item 1, wherein the antibacterial metal atom is silver. 3. The antibacterial cloth according to item 1, wherein the antibacterial metal atom is copper.