JPH0286770A - Production of powdery lactobacillus-plantarum - Google Patents
Production of powdery lactobacillus-plantarumInfo
- Publication number
- JPH0286770A JPH0286770A JP24009588A JP24009588A JPH0286770A JP H0286770 A JPH0286770 A JP H0286770A JP 24009588 A JP24009588 A JP 24009588A JP 24009588 A JP24009588 A JP 24009588A JP H0286770 A JPH0286770 A JP H0286770A
- Authority
- JP
- Japan
- Prior art keywords
- plantarum
- powder
- lactobacillus plantarum
- drying
- lactobacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 9
- 240000006024 Lactobacillus plantarum Species 0.000 title claims description 21
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims description 21
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims description 21
- 238000001035 drying Methods 0.000 claims abstract description 13
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims abstract description 12
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims abstract description 12
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims abstract description 12
- 239000003223 protective agent Substances 0.000 claims abstract description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 11
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract description 7
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229920002774 Maltodextrin Polymers 0.000 claims abstract description 6
- 239000005913 Maltodextrin Substances 0.000 claims abstract description 6
- 229940035034 maltodextrin Drugs 0.000 claims abstract description 6
- 241000186660 Lactobacillus Species 0.000 claims abstract 3
- 229940039696 lactobacillus Drugs 0.000 claims abstract 3
- 239000000843 powder Substances 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 6
- 239000004927 clay Substances 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 14
- 238000012258 culturing Methods 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract 4
- 229920001353 Dextrin Polymers 0.000 abstract 1
- 239000004375 Dextrin Substances 0.000 abstract 1
- 235000019425 dextrin Nutrition 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 238000003860 storage Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- TWWDDFFHABKNMQ-UHFFFAOYSA-N oxosilicon;hydrate Chemical compound O.[Si]=O TWWDDFFHABKNMQ-UHFFFAOYSA-N 0.000 description 3
- 238000001694 spray drying Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- BYFGZMCJNACEKR-UHFFFAOYSA-N aluminium(i) oxide Chemical compound [Al]O[Al] BYFGZMCJNACEKR-UHFFFAOYSA-N 0.000 description 2
- 230000030833 cell death Effects 0.000 description 2
- 229910001873 dinitrogen Inorganic materials 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 229910052814 silicon oxide Inorganic materials 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、生きた粉末ラクトバチルス・プランタラムを
多（得るために、ラクトバチルス・プランタラムを培養
した処理液に、前記ラクトバチルス・プランタラムに対
する保護剤を混入した後、前記処理液を乾燥して粉末状
の製品を造る粉末ラクトバチルス・プランタラムの製法
に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] In order to obtain a large amount of live powdered Lactobacillus plantarum, the present invention is directed to adding the Lactobacillus plantarum to a treatment solution in which Lactobacillus plantarum has been cultured. The present invention relates to a method for producing powdered Lactobacillus plantarum, which comprises mixing a protective agent against talum and then drying the treatment solution to produce a powdered product.
従来、保護剤として脱脂粉乳（ＭＳＮＦ）を使用するこ
とが考えられていた。Conventionally, it has been considered to use skimmed milk powder (MSNF) as a protective agent.
しかし、脱脂粉乳を混入した処理液を乾燥した場合の製
品中におけるラクトバチルス・プランタラムの生残率は
、乾燥直後で約３．７〜１２％と非常に低いという欠点
があった。However, when a treated solution mixed with skim milk powder is dried, the survival rate of Lactobacillus plantarum in the product immediately after drying is very low at about 3.7 to 12%.
本発明の目的は、製品中に右けるラクトバチルス・プラ
ンタラムの生残率を高める点にある。An object of the present invention is to increase the survival rate of Lactobacillus plantarum in a product.
本発明における粉末ラクトバチルス・プランタラムの製
法の特徴手段は、処理液に対する重量％として、
ポリビニルピロリドン（ＰＶＰ） ０．１〜２％グ
ルコース ３〜２０％マルトデキ
ストリンを８０重量％
とサイクロデキストリンを２０重
量％から成る混合物 ８〜３０％を、保護剤
に含有させておくことにあり、その作用・効果は次の通
りである。The characteristic means of the method for producing powdered Lactobacillus plantarum in the present invention is as follows: polyvinylpyrrolidone (PVP) 0.1-2% glucose 3-20% maltodextrin 80% by weight and cyclodextrin 20% by weight based on the treatment liquid The protective agent contains a mixture of 8 to 30% by weight, and its functions and effects are as follows.
つまり、乾燥直後の製品中のラクトバチルス・プランタ
ラムの生残率は、５０％以上というように従来の方法よ
りも高くなるばかりか、乾燥後の保存性においても１０
日目で２０％以上というように高く、活性の高い製品が
得られ、しかも、各種実験の結果、処理液に対して混入
する重量％として、
ポリビニルピロリドン（ＰＶＰ） ０．１％以下グ
ルコース ３％以下マルトデキスト
リンを８０重量％
とサイクロデキストリンを２０重
量％から成る混合物 ８％以下では、あまり
生残率を向上させる作用がなく、また、
ポリビニルピロリドン（ＰＶＰ） ２％以上グル
コース ２０％以上マルトデキスト
リンを８０重壷％
とサイクロデキストリンを２０重
量％から成る混合物 ３０％以上では、保護
剤自体が高価になると共に、乾燥に悪影響を及ぼす。In other words, the survival rate of Lactobacillus plantarum in the product immediately after drying is not only higher than that of the conventional method, at over 50%, but also the shelf life after drying is 10%.
A product with a high activity of 20% or more on the first day was obtained, and as a result of various experiments, the percentage by weight mixed in the treatment solution was as follows: Polyvinylpyrrolidone (PVP): 0.1% or less Glucose: 3% Below is a mixture consisting of 80% by weight of maltodextrin and 20% by weight of cyclodextrin.If it is less than 8%, it will not have much of an effect on improving the survival rate. A mixture consisting of 80% by weight and 20% by weight of cyclodextrin.If the content exceeds 30%, the protective agent itself becomes expensive and has a negative effect on drying.
従って、製品中のラクトバチルス・プランタラムの生残
率及び保存性を簡単に高めることができるために、生き
た粉末ラクトバチルス・プランタラムの生産効率を高め
てコストを下げることもできると共に、長期にわたる使
用が可能になった。Therefore, since the survival rate and shelf life of Lactobacillus plantarum in products can be easily increased, the production efficiency of live powder Lactobacillus plantarum can be increased and costs can be lowered, and the cost can be reduced over a long period of time. It has become possible to use it for a wide range of purposes.
次に、本発明の実施例を示す。 Next, examples of the present invention will be shown.
ラクトバチルス・プランタラム（Ｌａｃｔｏｂａｃｉ
Ｉｌｕｓｐｌａｎｔａｒｕｍ）をＬＣＭ培地で２４時間
培養した処理液に、処理液に対する重量％として、
ポリビニルピロリドン（ＰＶＰ） ０．１〜２％グ
ルコース ３〜２０％マルトデキ
ストリンを８０重量％
とサイクロデキストリンを２０重
量％から成る混合物 ８〜３０％を、ラクト
バチルス・プランタラムに対する保護剤として混入した
後、処理液をスプレードライヤーで噴霧乾燥するか、又
は、真空凍結乾燥を行って、粉末状の製品を造る。Lactobacillus plantarum
Polyvinylpyrrolidone (PVP) 0.1-2% Glucose 3-20% Maltodextrin 80% by weight and Cyclodextrin 20% by weight to the treatment solution obtained by culturing Ilusplantarum) in LCM medium for 24 hours. After incorporating 8-30% of a mixture consisting of the following as a protective agent against Lactobacillus plantarum, the treatment solution is spray-dried with a spray dryer or vacuum freeze-dried to produce a powdered product.
次に、前記製品中におけるラクトバチルス・プランタラ
ムの生残率を確認するための実験例を示す。Next, an experimental example for confirming the survival rate of Lactobacillus plantarum in the above product will be shown.
実験例
ポリビニルピロリドン（ＰＶＰ） ０．５ｗｔ１％
グルコース ｌＱｗｔ、％マルトデ
キストリンを８０重量％
とサイクロデキストリンを２０重
１％から成る混合物 １３ｗｔ１％を混入す
ると共に、回収率向上のために、更に、パウダー状の酸
化ケイ素（Ｓ１０２）水和物を１ｗｔ１％混入し、東京
理化器機製、５Ｏ−１型のスプレードライヤーで、表１
の条件下で噴霧乾燥して粉末状の製品を回収し、その粉
末中の生菌数を測定した。Experimental example Polyvinylpyrrolidone (PVP) 0.5wt1%
13wt1% of a mixture consisting of 80wt% maltodextrin and 20wt1% cyclodextrin is mixed in, and in order to improve the recovery rate, 1wt1 of powdered silicon oxide (S102) hydrate is added. Table 1
A powdered product was collected by spray drying under the following conditions, and the number of viable bacteria in the powder was measured.
次に、乾燥して得られた粉末を、３７℃の空気中で１０
日間保存した後の生菌数を測定して、保存性を確δ忍し
た。Next, the powder obtained by drying was dried in air at 37°C for 10 minutes.
The number of viable bacteria was measured after storage for several days to ensure shelf life.
上記結果は、表２に示す。The above results are shown in Table 2.
表２
尚、表２において、ラクトバチルス・プランタラムの生
残率を下記の式から求めた。Table 2 In Table 2, the survival rate of Lactobacillus plantarum was determined from the following formula.
つまり、上記実験より、乾燥直後の菌の生残率は良好で
あるばかりか、１０日保存後の生残率からも判るように
、乾燥後の保存性が優れている。In other words, from the above experiment, not only the survival rate of bacteria immediately after drying is good, but also the storage stability after drying is excellent, as can be seen from the survival rate after 10 days of storage.
前記保護剤中の、グルコースに関し、処理液に対して混
入した時の生残率又は菌体死滅速度の変化傾向を示すグ
ラフを、次に示す。Regarding glucose in the protective agent, a graph showing a change trend in the survival rate or bacterial cell death rate when it is mixed into the treatment liquid is shown below.
グルコースの濃度変化に対する乾燥器内での菌体死滅速
度の変化傾向は、乾燥器の人口温度が１８０℃の時（第
１図）と１５０℃の時（第２図）とでは異なり、乾燥後
の保存日数の変化に伴う生菌数の変化は、グルコースの
濃度変化（例えば２％、５％、１０％）に伴って異なる
ばかりか、乾燥器の人口温度が、１８０℃の時（第３図
）と、１５０℃の時（第４図）とでは異なる。前記第１
図乃至第４図より乾燥器の入口温度が高い方が保存性が
悪くなると共に、菌体死滅速度は、乾燥器人口温度が１
５０℃の場合は、グルコースの濃度の増大に伴って遅く
なり、１８０℃の場合は、グルコース濃度５ｗｔ、％の
時が一番遅く、また、空気中よりも窒素ガス中の方が菌
体は死滅しにくいことが判る。The change trend in the killing rate of bacterial cells in the dryer with respect to changes in glucose concentration differs when the population temperature of the dryer is 180°C (Fig. 1) and 150°C (Fig. 2). The change in the number of viable bacteria due to the change in the number of days of storage of the (Fig. 4) is different from that at 150°C (Fig. 4). Said first
From Figures 4 to 4, it can be seen that the higher the dryer inlet temperature is, the worse the storage stability is, and the bacterial cell death rate is lower than the dryer population temperature.
At 50°C, the rate slows down as the glucose concentration increases, and at 180°C, the slowest rate is when the glucose concentration is 5wt%, and bacterial cells grow faster in nitrogen gas than in air. It turns out that it is hard to die.
前記処理液の乾燥は、噴霧乾燥による例を示したが、凍
結乾燥を行っても良く、表６に凍結乾燥を行った時の生
残率を示す。Although spray drying was used as an example of drying the treatment liquid, freeze-drying may also be used, and Table 6 shows the survival rate when freeze-drying was performed.
表３
ただし、乾燥条件は真空度２ Ｘ １０− ’Ｔｏｒｒ
、温度室温（約２０℃）、サンプル凍結温度は、−８０
℃で２４時間、保存条件は３７℃の窒素ガス中で保存し
た。ただし、噴霧乾燥（スプレードライ）の方が、凍結
乾燥よりも乾燥粉末の生産効率が良く、経済的であるば
かりか、ラクトバチルス・プランタラムの生残率も良い
ために実用的である。Table 3 However, the drying conditions are vacuum degree 2 x 10-'Torr.
, temperature room temperature (approximately 20℃), sample freezing temperature is -80℃
It was stored at 37°C in nitrogen gas for 24 hours. However, spray drying is not only more efficient and economical in producing dry powder than freeze drying, but also has a good survival rate for Lactobacillus plantarum, so it is practical.
前記処理液に混入させる保護剤の個々の成分比率は、い
ろいろ実験した結果、前記範囲が適し、夫々が最低割合
（ｗｔ０％）以下では、生残率を高める効果が少なくな
り、また、最高割合（ｗｔ、％）以上では、高価になっ
て経済的に不利になるばかりか、乾燥効率の低下を招く
虞があり、望むべくは、前記実験例で示したように、Ｐ
Ｖ Ｐ ０．５ｗｔ、％グルコー
ス ｌＱｗｔ６％前記混合物
１３ｗｔ、％酸化ケイ素水和物 １ｗｔ、
％が良いが、上記以外の成分が保護剤中に含まれていて
も良い。As a result of various experiments, the ratio of each component of the protective agent to be mixed into the treatment solution was found to be within the range described above.If each component is below the minimum ratio (wt 0%), the effect of increasing the survival rate will be small; (wt, %) or more, it not only becomes expensive and economically disadvantageous, but also may lead to a decrease in drying efficiency.
V P 0.5wt,% glucose lQwt 6% of the above mixture
13wt, % silicon oxide hydrate 1wt,
%, but components other than those listed above may be included in the protective agent.
前記処理液中に、酸化ケイ素水和物の微小粉末に代えて
、ｐｅ２０：＋粉末、Ａｌ２Ｏ＊粉末、ガラス粉、珪砂
微粉末、及び、クレー粉末のうちから選ばれた少なくと
も一種を、１〜５ｉｖｔ０％混入させても、粉末製品の
回収率は向上する。In place of the fine powder of silicon oxide hydrate, at least one selected from pe20:+ powder, Al2O* powder, glass powder, silica sand fine powder, and clay powder is added to the treatment liquid in place of the fine powder of silicon oxide hydrate. Even when 5ivt is mixed in at 0%, the recovery rate of the powdered product is improved.
図面は本発明に係る粉末ラクトバチルス・プランタラム
の製法に関し、第１図及び第２図は夫々菌体死減速度を
示すグラフ、第３図及び第４図は夫々生菌数の変化を示
すグラフである。The drawings relate to the method for producing powdered Lactobacillus plantarum according to the present invention, and FIGS. 1 and 2 are graphs showing the bacterial death rate, respectively, and FIGS. 3 and 4 are graphs showing changes in the number of viable bacteria, respectively. It is a graph.
Claims (1)
、前記ラクトバチルス・プランタラムに対する保護剤を
混入した後、前記処理液を乾燥して粉末状の製品を造る
粉末ラクトバチルス・プランタラムの製法であって、前
記処理液に対する重量％として、 ポリビニルピロリドン（ＰＶＰ）０．１〜２％グルコー
ス３〜２０％ マルトデキストリンを８０重量％ とサイクロデキストリンを２０重 量％から成る混合物８〜３０％ を、前記保護剤に含有させておく粉末ラクトバチルス・
プランタラムの製法。 ２、ＳｉＯ＿２粉末、Ｆｅ＿２Ｏ＿３粉末、Ａｌ＿２Ｏ
＿３粉末、ガラス粉末、及び、クレー粉末の中から選ば
れた少なくとも一種を、前記処理液に対して１〜５重量
％の割合で混入する請求項１記載の粉末ラクトバチルス
・プランタラムの製法。[Scope of Claims] 1. Powdered Lactobacillus plantarum produced by mixing a protective agent against Lactobacillus plantarum into a treatment solution in which Lactobacillus plantarum is cultured, and then drying the treatment solution to produce a powdered product.・A method for producing plantarum, in which the weight percent of the treatment liquid is: polyvinylpyrrolidone (PVP) 0.1-2% glucose 3-20% maltodextrin 80% by weight and cyclodextrin 20% by weight a mixture 8 ~30% of the powdered Lactobacillus powder contained in the protective agent.
How to make plantarum. 2. SiO_2 powder, Fe_2O_3 powder, Al_2O
The method for producing powdered Lactobacillus plantarum according to claim 1, wherein at least one selected from _3 powder, glass powder, and clay powder is mixed in the treatment liquid at a ratio of 1 to 5% by weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24009588A JPH0286770A (en) | 1988-09-26 | 1988-09-26 | Production of powdery lactobacillus-plantarum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24009588A JPH0286770A (en) | 1988-09-26 | 1988-09-26 | Production of powdery lactobacillus-plantarum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0286770A true JPH0286770A (en) | 1990-03-27 |
Family
ID=17054417
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24009588A Pending JPH0286770A (en) | 1988-09-26 | 1988-09-26 | Production of powdery lactobacillus-plantarum |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0286770A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6010725A (en) * | 1996-07-09 | 2000-01-04 | Nestec S.A. | Spray-drying process |
| JP2005052100A (en) * | 2003-08-06 | 2005-03-03 | Shuichi Shiomi | Dried material of lactic acid bacterium and method for producing the same |
| IT201700062260A1 (en) * | 2017-06-07 | 2018-12-07 | Proge Farm Srl | ASSOCIATION AND COMPOSITIONS TOPICAL SKIN INCLUDING LACTOBACILLI AND METALLIC AND / OR SEMI-METAL OXIDES |
-
1988
- 1988-09-26 JP JP24009588A patent/JPH0286770A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6010725A (en) * | 1996-07-09 | 2000-01-04 | Nestec S.A. | Spray-drying process |
| CN1074255C (en) * | 1996-07-09 | 2001-11-07 | 雀巢制品公司 | Spray-drying process |
| JP2005052100A (en) * | 2003-08-06 | 2005-03-03 | Shuichi Shiomi | Dried material of lactic acid bacterium and method for producing the same |
| IT201700062260A1 (en) * | 2017-06-07 | 2018-12-07 | Proge Farm Srl | ASSOCIATION AND COMPOSITIONS TOPICAL SKIN INCLUDING LACTOBACILLI AND METALLIC AND / OR SEMI-METAL OXIDES |
| WO2018224995A1 (en) * | 2017-06-07 | 2018-12-13 | Proge Farm S.R.L. | Combination and topical compositions comprising lactic bacteria and metal and/or semimetal oxides |
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