JPH0286767A - Production of powdery lactobacillus-plantarum - Google Patents
Production of powdery lactobacillus-plantarumInfo
- Publication number
- JPH0286767A JPH0286767A JP63239290A JP23929088A JPH0286767A JP H0286767 A JPH0286767 A JP H0286767A JP 63239290 A JP63239290 A JP 63239290A JP 23929088 A JP23929088 A JP 23929088A JP H0286767 A JPH0286767 A JP H0286767A
- Authority
- JP
- Japan
- Prior art keywords
- lactobacillus plantarum
- powder
- plantarum
- drying
- lactobacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 10
- 240000006024 Lactobacillus plantarum Species 0.000 title claims description 27
- 235000013965 Lactobacillus plantarum Nutrition 0.000 title claims description 26
- 229940072205 lactobacillus plantarum Drugs 0.000 title claims description 26
- 239000005862 Whey Substances 0.000 claims abstract description 15
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 15
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 15
- 238000001035 drying Methods 0.000 claims abstract description 13
- 239000003223 protective agent Substances 0.000 claims abstract description 10
- 239000000843 powder Substances 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 14
- 239000004927 clay Substances 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 16
- 241000186660 Lactobacillus Species 0.000 abstract 3
- 229940039696 lactobacillus Drugs 0.000 abstract 3
- 238000012258 culturing Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 description 12
- 241000894006 Bacteria Species 0.000 description 11
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 7
- 230000007423 decrease Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000011084 recovery Methods 0.000 description 4
- 229910052814 silicon oxide Inorganic materials 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- 238000001694 spray drying Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229910052593 corundum Inorganic materials 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- TWWDDFFHABKNMQ-UHFFFAOYSA-N oxosilicon;hydrate Chemical compound O.[Si]=O TWWDDFFHABKNMQ-UHFFFAOYSA-N 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 229910001845 yogo sapphire Inorganic materials 0.000 description 1
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
生きた粉末ラクトバチルス・プランタラムを多く得るた
めに、ラクトバチルス・プランタラムを培養した処理液
に、前記ラクトバチルス・プランタラムに対する保護剤
を混入した後、前記処理液を乾燥して粉末状の製品を造
る粉末ラクトバチルス・プランタラムの製法に関する。[Detailed Description of the Invention] [Industrial Application Field] In order to obtain a large amount of live powder Lactobacillus plantarum, a protective agent against Lactobacillus plantarum is added to the treatment solution in which Lactobacillus plantarum is cultured. The present invention relates to a method for producing powdered Lactobacillus plantarum, which comprises mixing and drying the treatment liquid to produce a powdered product.
従来、保護剤として脱脂粉乳（ＭＳＮＦ）を使用するこ
とが考えられていた。Conventionally, it has been considered to use skimmed milk powder (MSNF) as a protective agent.
しかし、脱脂粉乳を混入した処理液を乾燥した場合の製
品中にふけるラクトバチルス・プランタラムの生残率は
、乾燥直後で約３．７〜１２％と非常に低いという欠点
があった。However, when a treated solution mixed with skim milk powder is dried, the survival rate of Lactobacillus plantarum in the product immediately after drying is very low, about 3.7 to 12%.
本発明の目的は、製品中におけるラクトバチルス・プラ
ンタラムの生残率を高める点にある。An object of the present invention is to increase the survival rate of Lactobacillus plantarum in a product.
本発明における粉末ラクトバチルス・プランタラムの製
法の特徴手段は、処理液に対して８〜３０重量％のホエ
ーを、保護剤に含有させておくことにあり、その作用・
効果は次の通りである。The characteristic means of the method for producing powdered Lactobacillus plantarum in the present invention is that the protective agent contains 8 to 30% by weight of whey based on the processing liquid,
The effects are as follows.
つまり、乾燥直後の製品中のラクトバチルス・プランタ
ラムの生残率は、図に示すように、例えば処理液に対す
るホエーの重量％が８％で約７０％の生残率を示し、ホ
エーが１３重重量以上では、はぼ１００％の生存率を示
して、従来よりも製品中におけるラクトバチルス・プラ
ンタラムの生残率を高められる。ただし、いろいろ実験
した結果ホエーが処理液に対して８重量％以下の場合は
、保存性が悪くて日数経過に伴って生残率の低下が大き
くて、実用的ではなく、また、ホエーが処理液に対して
３０重重量以上になると、高価になると共に、処理液の
乾燥効率が低下する。In other words, as shown in the figure, the survival rate of Lactobacillus plantarum in the product immediately after drying is approximately 70% when the weight percentage of whey in the treatment liquid is 8%, and the survival rate of Lactobacillus plantarum in the product immediately after drying is approximately 70%. When the weight is over 100%, the survival rate of Lactobacillus plantarum in the product can be increased compared to the conventional method. However, as a result of various experiments, if the whey content is less than 8% by weight based on the processing solution, the storage stability is poor and the survival rate decreases significantly as days pass, making it impractical. When the weight exceeds 30% by weight relative to the liquid, it becomes expensive and the drying efficiency of the processing liquid decreases.
従って、製品中のラクトバチルス・プランタラムの生残
率を簡単に高めることができるために、生きた粉末ラク
トバチルス・プランタラムの生産効率を高めてコストを
下げることもてきるようになった。Therefore, since the survival rate of Lactobacillus plantarum in a product can be easily increased, it has become possible to increase the production efficiency of live powdered Lactobacillus plantarum and reduce costs.
次に、本発明の実施例を示す。 Next, examples of the present invention will be shown.
ラクトバチルス・プランタラム（Ｌａｃｔｏｂａｃｉｌ
ｌｕｓｐｌａｎｔａｒｕｍ）をＬＣＭ培地で２４時間培
養した処理液に、チーズ又はカゼイン製造の副産物とし
て得られるホエー（ｗｈｅｙ）を、ラクトバチルス・プ
ランタラムの保護剤として、処理液に対して８〜３０重
量％（望ましくは１６重量％）の割合で混入し、その後
、処理液をスプレードライヤーで噴霧乾燥するか、又は
、真空凍結乾燥を行って、粉末状の製品を造る。Lactobacillus plantarum
Lactobacillus plantarum) was cultured in LCM medium for 24 hours, and whey obtained as a by-product of cheese or casein production was added to the treatment solution as a protective agent for Lactobacillus plantarum at 8 to 30% by weight ( The treatment solution is then spray-dried using a spray dryer or vacuum freeze-dried to produce a powdered product.
次に、前記製品中におけるラクトバチルス・プランタラ
ムの生残率を、確認するための実験例を示す。Next, an experimental example for confirming the survival rate of Lactobacillus plantarum in the above product will be shown.
実験例１
ラクトバチルス・プランタラムに対する保護剤としてホ
エーを、処理液に対して１５ｗｔ、％混入し、次の表１
の条件のスプレードライヤー（東京理化器機製、５Ｏ−
１型）で噴霧乾燥して粉末を回収し、その粉末中の生菌
数を測定した。Experimental Example 1 Whey was mixed as a protective agent against Lactobacillus plantarum at 15 wt% to the treatment liquid, and the following Table 1 was added.
Spray dryer (manufactured by Tokyo Rikakiki, 5O-
The powder was collected by spray drying using Type 1), and the number of viable bacteria in the powder was measured.
表１
次に、乾燥して得られた粉末を、３７℃の空気中で１０
日間保存した後の生菌数を測定して、保存性を確認した
。Table 1 Next, the powder obtained by drying was dried in air at 37°C for 10 minutes.
Preservability was confirmed by measuring the number of viable bacteria after storage for days.
上記結果は、表２に示す。The above results are shown in Table 2.
尚、表２において、ラクトバチルス・プランタラムの生
残率を下記の式から求めた。In Table 2, the survival rate of Lactobacillus plantarum was determined from the following formula.
尚、前記ホエーはパウダー状で、その成分は、表３に示
す。Note that the whey is in powder form, and its components are shown in Table 3.
表３
脂 肪 １．０％
水 分 ３．０％
蛋白質 １２．０％
炭水化物 ７６．０％（乳糖が主成分）灰 分
８．０％
一般生菌数 １０００個７ｇ以下
大腸菌群 陰性
実験例２
ラクトバチルス・プランタラムの処理液に、前記ホエー
を１５ｗｔ、％、酸化ケイ素人和物の微小粉末を、１．
Ｑｗｔ、％混入させた後、実験例１と同様にスプレード
ライヤーで噴霧乾燥して粉末を造り、その粉末中の生菌
数を測定した。Table 3 Fat 1.0% Water 3.0% Protein 12.0% Carbohydrate 76.0% (lactose is the main component) Ash 8.0% General viable bacteria count 1000 pieces 7g or less Coliform bacteria Negative experiment example 2 1. 15 wt% of the above whey and fine powder of silicon oxide human salt were added to the Lactobacillus plantarum treatment solution.
After mixing Qwt%, powder was prepared by spray drying with a spray dryer in the same manner as in Experimental Example 1, and the number of viable bacteria in the powder was measured.
次に、前記粉末を３７℃の空気中で１０日間保存した後
の生菌数を測定して、保存性を確認した。Next, the powder was stored in the air at 37° C. for 10 days, and the number of viable bacteria was measured to confirm the shelf life.
上記結果は、表４に示す。The above results are shown in Table 4.
表 ５
つまり、上記実験例１、実験例２で示すように、乾燥直
後の菌の生残率は良好で、特に酸化ケイ集水和物を混入
した場合は、菌の回収率が向上して、乾燥後の生存率が
高くなったと見られる。Table 5 In other words, as shown in Experimental Examples 1 and 2 above, the survival rate of bacteria immediately after drying was good, and the recovery rate of bacteria was particularly improved when hydrated silicon oxide was mixed. , the survival rate after drying seems to have increased.
そこで、次に酸化ケイ集水和物の混入率（ｗｔ、％）の
差による乾燥後の回収率の変化を、次の表５に示す。Therefore, the following Table 5 shows the change in the recovery rate after drying due to the difference in the mixing rate (wt, %) of the silicon oxide condensate hydrate.
ただし、スプレードライヤーは、アシザワ・二ロアトマ
イザー鞠社製のＰＭ型を使用する。However, the spray dryer used is a PM type manufactured by Ashizawa Niro Atomizer Mari Co., Ltd.
上記表５から酸化ケイ素水和物の混入率は、高いほど乾
燥生成物の回収率が良いことが判る。From Table 5 above, it can be seen that the higher the mixing rate of silicon oxide hydrate, the better the recovery rate of the dry product.
前記処理液の乾燥は、噴霧乾燥による例を示したが、凍
結乾燥を行っても良く、ただし噴霧乾燥（スプレードラ
イ）の方が、凍結乾燥よりも乾燥粉末の生産効率が良く
、経済的であるばかりか、ラクトバチルス・プランタラ
ムの生残率も良いために実用的である。Although an example of drying the treatment liquid is performed by spray drying, freeze drying may also be performed, however, spray drying is more efficient and economical in producing dry powder than freeze drying. Not only that, but the survival rate of Lactobacillus plantarum is also high, making it practical.
前記処理液に混入させるホエーは、図のグラフに示すよ
うに、処理液に対して３ｗｔ、％混入させると、ラクト
バチルス・プランタラムが約７０％の生残率を示すが、
数日後の生菌数の減少が大きいために、処理液に対する
混入量としては３ｗｔ、％以上が良く、また、約１３ｗ
ｔ、％のホエー混入で生残率が１００％近くなるものの
、確実性を求めるために、ホエーを１５ｗｔ、％以上処
理液に混入させるのが望ましい。As shown in the graph in the figure, when the whey mixed into the treatment liquid is mixed at 3wt% to the treatment liquid, Lactobacillus plantarum shows a survival rate of about 70%.
Since the number of viable bacteria decreases significantly after a few days, it is best to mix the amount of bacteria into the treatment solution at 3 wt.% or more, and about 13 w.
Although the survival rate approaches 100% when whey is mixed in at a concentration of 15 wt.%, it is desirable to mix 15 wt.% or more of whey into the treatment liquid in order to obtain reliability.
また、ホエーが３Ｑｗｔ、％以上処理液に対して混入さ
せる場合には、乾燥効率が低下するばかりかコストが高
くなって、粉末ラクトバチルス・プランタラムの製造コ
ストが、製品単価に対して大きな比率を占めやすく、実
用性が低くなる。In addition, when whey is mixed into the processing liquid by 3Qwt% or more, not only the drying efficiency decreases but also the cost increases, and the manufacturing cost of powdered Lactobacillus plantarum becomes a large proportion of the product unit price. This makes it less practical.
前記処理液中に、酸化ケイ集水和物の微小粉末に代えて
、Ｆｅ２Ｏ３粉末、Ａｌ２Ｏ３粉末、ガ−）ス粉、珪砂
微粉末、及び、クレー粉末のうちから選ばれた少なくと
も一種を、ｌ ｗｔ、％〜５ｗｔ１％混入させても、粉
末製品の回収率は向上する。In place of the fine powder of hydrated silicon oxide, at least one selected from Fe2O3 powder, Al2O3 powder, gas powder, silica sand fine powder, and clay powder is added to the treatment liquid. Even if it is mixed in wt,% to 5wt1%, the recovery rate of the powder product is improved.
前記保護剤にはホエー以外の成分が含まれていても良い
。The protective agent may contain components other than whey.
図面は菌の生残率を示すグラフである。 The figure is a graph showing the survival rate of bacteria.
Claims (1)
、前記ラクトバチルス・プランタラムに対する保護剤を
混入した後、前記処理液を乾燥して粉末状の製品を造る
粉末ラクトバチルス・プランタラムの製法であって、前
記処理液に対して８〜３０重量％のホエーを、前記保護
剤に含有させておく粉末ラクトバチルス・プランタラム
の製法。 ２、ＳｉＯ＿２粉末、Ｆｅ＿２Ｏ＿３粉末、Ａｌ＿２Ｏ
＿３粉末、ガラス粉末、及び、クレー粉末の中から選ば
れた少なくとも一種を、前記処理液に対して１〜５重量
％の割合で混入する請求項１記載の粉末ラクトバチルス
・プランタラムの製法。[Scope of Claims] 1. Powdered Lactobacillus plantarum produced by mixing a protective agent against Lactobacillus plantarum into a treatment solution in which Lactobacillus plantarum is cultured, and then drying the treatment solution to produce a powdered product. - A method for producing powdered Lactobacillus plantarum, in which the protective agent contains whey in an amount of 8 to 30% by weight based on the treatment liquid. 2. SiO_2 powder, Fe_2O_3 powder, Al_2O
The method for producing powdered Lactobacillus plantarum according to claim 1, wherein at least one selected from _3 powder, glass powder, and clay powder is mixed in the treatment liquid at a ratio of 1 to 5% by weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63239290A JPH0286767A (en) | 1988-09-24 | 1988-09-24 | Production of powdery lactobacillus-plantarum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63239290A JPH0286767A (en) | 1988-09-24 | 1988-09-24 | Production of powdery lactobacillus-plantarum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0286767A true JPH0286767A (en) | 1990-03-27 |
Family
ID=17042535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63239290A Pending JPH0286767A (en) | 1988-09-24 | 1988-09-24 | Production of powdery lactobacillus-plantarum |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0286767A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6010725A (en) * | 1996-07-09 | 2000-01-04 | Nestec S.A. | Spray-drying process |
| JP2016537017A (en) * | 2013-10-28 | 2016-12-01 | セーホーエル．ハンセン アクティーゼルスカブ | Microbial drying |
-
1988
- 1988-09-24 JP JP63239290A patent/JPH0286767A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6010725A (en) * | 1996-07-09 | 2000-01-04 | Nestec S.A. | Spray-drying process |
| JP2016537017A (en) * | 2013-10-28 | 2016-12-01 | セーホーエル．ハンセン アクティーゼルスカブ | Microbial drying |
| US10745661B2 (en) | 2013-10-28 | 2020-08-18 | Chr. Hansen A/S | Drying of microorganisms |
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