JPH02288818A - Cosmetic containing treated material of shikonins - Google Patents

Abstract

PURPOSE:To obtain a cosmetic containing treated material of shikonins obtained by subjecting shikonins to blending treatment with a specific metal ion in water soluble solvent solution of protein and/or peptide and free from care of allergy. CONSTITUTION:The cosmetic obtained by blending a treated material obtained by subjecting shikonins to blending treatment with a solution of one kind of metal in aluminum, iron, magnesium, zinc and copper in a water soluble solvent solution of protein or peptide or blend of protein and peptide. Shikonin treated by the above-mentioned method or derivative thereof or blend thereof exhibits red or red-purple or blue-purple and shows no contact allergic action and is most preferably used as a coloring agent or pigment for cosmetic.

Description

Detailed Description of the Invention (a) Purpose of the Invention The present invention aims to reduce the contact allergic effect of shikonins on the skin when they are used as colorants or pigments in skin cosmetics. This invention relates to highly safe cosmetics that eliminate the expression of

(Industrial Application Field) The shikonin or its derivative according to the present invention does not exhibit contact allergic effects even if it is directly attached to the skin.
Therefore, it can be frequently used as a coloring agent or pigment in cosmetics in general.

In addition, the means adopted for eliminating contact allergy caused by shikonin or shikonin derivatives according to the present invention is to use a treatment product or composition by mixing and stirring a specific metal ion together with proteins or peptides. In particular, if this method is used, various other synthetic pigments or colorants, or other drugs used as raw materials for cosmetic formulations, which are known to induce contact allergies, can be removed. It can be used as an effective pretreatment means to protect against this in the formulation of cosmetics.

"Table 1" Main shikonin derivatives (shikonins) (prior art) Shikonin or shikonin derivatives are known as one of the pigment components contained in the roots of purple grass, and have pharmacological effects such as anti-inflammatory and sedative effects. It is known for its antibacterial and granulation-promoting effects.

On the other hand, the rhizomes of purple grass have a long history of being used as dyes for clothing fibers, and furthermore,
Extracted Egis, whose true color is the pigment, is blended into cosmetics.

When using Shikonin extract in cosmetics, its purpose can be roughly divided into two types. One of them is the expectation of the pharmacological action of Shikonin, etc. mentioned above, and the other is to use it as a coloring agent or pigment. It was used as a.

(Known Publications) Known publications regarding shikonin, shikonin derivatives, or rhizomes of purple violet (hereinafter referred to as shikon in accordance with the Chinese medicine name) include, for example, the following publication published by the Japan Patent Office.

(1) Published Patent Publication 1984-49409 (*)(2
) Published Patent Publication 1987-94432 (*) (3) Published Patent Publication 1987-10507 (*) (4) Published Patent Publication 1982-58909 (5) Published Patent Publication 1982
O-58910 (6) Published Patent Publication 1986-5891
1 (7) Published Patent Publication 1982-28279 (8) Published Patent Publication 1982-28278 Among the above publications, (1 to 3)
) is by the present inventors.

Conventionally, in cosmetics, when used as a coloring agent or pigment, a liquid version of Shikonisokusu extract has been used, for example, the product shown in the above-mentioned published patent publication (1) (Shikonisox liquid, - round). (manufactured by Fudelcos), 02 to 05% is used as an appropriate amount, and at this amount, the pharmacological action is mild, and there are no problems when used as a coloring agent for cosmetics.

(Problems to be Solved by the Invention) Shikonins can now be produced by the tissue culture method of Shikonins, as shown in the publications (7-8), and based on this, they can be mass-produced (extraction and isolation). ) became possible.

The separated shikonin or shikonin derivative has strong pharmacological activity, unlike conventional shikonin extract, and when it is incorporated into cosmetics as a coloring agent or pigment,
In addition to the pharmacological action of shikonin, it is necessary to take into consideration the fact that it induces a contact allergic action on the skin, which is a contradictory side effect, but this point has not been well known in the past.

Until now, no means have been disclosed to prevent the induction of contact allergies when using shikonin as a colorant or pigment in cosmetics.

The inventors of the present invention began research to solve this problem while conducting re-evaluation tests regarding pharmacological effects and their safety, and were able to complete the present invention.

(B) Structure of the Invention The present invention provides a method for preparing shikonins (for shikonin or shikonin derivatives) in an aqueous hot medium solution of a specified metal ion and a protein, a peptide, or a mixture of a protein and a peptide. obtained by mixing,
A processed product of shikonins is used by containing it in cosmetics.

(Means for solving the problem) "Example 1" Shikonin bulk powder, shikonin derivatives (shown in Table 1), or a mixture thereof is dissolved in ethanol or a mixture of methanol and water. 2% aluminum chloride methanol solution, 1% ferric chloride ethanol solution, zinc (Zn゛) solution, magnesium (Mg
``) solution, copper (Cu '') solution, any one type of metal ion solution, and further water-soluble protein, or
There is no particular limitation as long as it is a water-soluble peptide or a mixture of a water-soluble protein and a water-soluble peptide, but examples include vegetable wheat gluten, soy milk that is soy protein, animal albumin, serum, Gasein, gelatin, placenta extract, or an aqueous solution of low-molecular water-soluble proteins or peptides obtained by hydrolysis or enzymatic decomposition of placenta, collagen, elastin, keratin, fibroin, sericin, etc. Stir.

Next, 0. A rapid precipitate is formed by adding IN sodium hydroxide solution and adjusting the pH of the mixed stirring solution to 7-8. Therefore, this precipitate is removed, thoroughly washed with water, and then incorporated into various known cosmetics. or,
The precipitate is fine (washed with water, dried and stored as a powder,
If necessary, it may be used by blending it into cosmetics, and after thoroughly washing the precipitate with water and dissolving it in a 0.1 to 0.2N sodium hydroxide solution, further using a 2N hydrochloric acid solution. It is neutralized and mixed in a polyol solvent such as PEG (polyethylene glycol), propylene glycol, butylene glycol, water, ethanol, or water and ethanol, and then blended into cosmetics. "Example 2" which may be used Powder material mainly composed of water-insoluble proteins, e.g.
Animal-derived proteins such as water-insoluble protein derived from vegetable soybeans, silk powder, fibroin, sericin collagen, elastin, and keratin are mixed with a 5% aluminum acetate aqueous solution, iron (Fe'''''''), and zinc. (
Add to one type of metal ion solution of Zn~), magnesium (Mg"), and copper (Cu-), stir and disperse, then set aside overnight, filter, wash with water, and collect the precipitate. .

Next, the precipitate obtained earlier was added to a solution in which shikonin, its derivatives (shown in Table 1), or a mixture thereof were dissolved in ethanol or a mixture of methanol and water. , in a hot bath at 60 to 70°C, with occasional stirring,
Heating is performed for about 90 minutes. After heating, let it stand, separate the precipitate, dehydrate it, wash it with water, and immerse it in ethanol.
The precipitate is collected, air-dried, and then dried under reduced pressure at 80±5°C to form a powder. This powder is blended into any cosmetic.

(c) Effects of the invention Shikonin or its derivatives treated in Example 1 or 2 of the preceding section, or a mixture thereof exhibits a red, reddish-purple, or bluish-purple color and does not exhibit a contact allergic effect on the skin; Ideal for use as a young colorant or pigment in cosmetics.

The effectiveness of the present invention is determined based on the Maximization Method (Reference: New Toxicity Test and Safety Evaluation, published by Soft Science), as well as Takase's method (Reference: Fragrance Journal N).

14 Fragrance Journal Co., Ltd., 1975), a guinea pig was used as the test animal, and the sensitization method was to use shikonin and administer it to the cervix by sarcastic injection. The precipitate after treatment was used as a specimen, and the test was carried out by blending this into a parent wood ointment and applying it. In addition, the application group was conducted with 5 animals per group.

The following table (Table 2) shows the results of the precipitates treated with shikonin according to Example 1 or 2. Second
As shown in the table, no skin contact allergic effects were observed.

Considering the effect on this treatment from the structural perspective of shikonin, the active group that has pharmacological action is found in -OH and =O from the structural formula of shikonin as shown below. This -OH and 20 are expected to be one of the triggers for the development of skin contact allergies, irritating the skin. In other words, -OH and =0 were in a state where it was easy to act on (easily bond to) the proteins that make up the skin.

Therefore, in Examples 1 and 2, by reacting with various proteins and peptides in place of biological skin constituent proteins in advance, contact allergies will not occur even if applied directly to the skin. It is estimated that this is no longer the case.

Further, in Examples 1 and 2, iron, aluminum, or the like was used as the specified metal ion solution, but even if iron or aluminum is not used, the intended contact allergy effect will not be exhibited. However, when added to cosmetics and subjected to heating treatment, such as emulsification or cream,
Or when used in large quantities, tests using the cream or emulsion can sometimes show skin contact allergies. However, when specific metal ions were used as in Examples 1 and 2, this was not observed.

If we consider this point further, perhaps (
For example, in contrast to −〇 H and 20, iron and aluminum have an effective bonding state with the histidine group present in other proteins and peptides, and form active groups such as 1OH and =0. It is presumed that the enzyme acts on the inactivation of .

It has been found that the treatment method according to the present invention can also be used as a means for suppressing skin contact allergies to other quinone pigments.

Claims (1)

[Claims] (1) Shikonins contain aluminum, iron, and metal ions as metal ions.
Contains a processed product of shikonins obtained by mixing a solution of one of magnesium, zinc, and copper with a protein or peptide, or a mixture of protein and peptide in an aqueous solvent solution. A cosmetic product characterized by: