JPH02280893A - Method for immobilizing nitrifying bacteria and water purifying treatment - Google Patents
Method for immobilizing nitrifying bacteria and water purifying treatmentInfo
- Publication number
- JPH02280893A JPH02280893A JP10133489A JP10133489A JPH02280893A JP H02280893 A JPH02280893 A JP H02280893A JP 10133489 A JP10133489 A JP 10133489A JP 10133489 A JP10133489 A JP 10133489A JP H02280893 A JPH02280893 A JP H02280893A
- Authority
- JP
- Japan
- Prior art keywords
- nitrifying bacteria
- beads
- water
- added
- activated carbon
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000001546 nitrifying effect Effects 0.000 title claims abstract description 48
- 241000894006 Bacteria Species 0.000 title claims abstract description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 29
- 230000003100 immobilizing effect Effects 0.000 title claims description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000011324 bead Substances 0.000 claims abstract description 16
- 239000011347 resin Substances 0.000 claims abstract description 11
- 229920005989 resin Polymers 0.000 claims abstract description 11
- 238000003756 stirring Methods 0.000 claims abstract description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 7
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 7
- 239000001110 calcium chloride Substances 0.000 claims abstract description 7
- 238000004132 cross linking Methods 0.000 claims abstract description 6
- 239000010802 sludge Substances 0.000 claims abstract description 6
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 32
- 229910021529 ammonia Inorganic materials 0.000 claims description 16
- 238000001914 filtration Methods 0.000 claims description 15
- 238000000746 purification Methods 0.000 claims description 11
- 239000002250 absorbent Substances 0.000 claims description 4
- 230000002745 absorbent Effects 0.000 claims description 4
- 239000012141 concentrate Substances 0.000 claims description 3
- UYJXRRSPUVSSMN-UHFFFAOYSA-P ammonium sulfide Chemical compound [NH4+].[NH4+].[S-2] UYJXRRSPUVSSMN-UHFFFAOYSA-P 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 abstract description 2
- 239000006228 supernatant Substances 0.000 abstract description 2
- 238000005406 washing Methods 0.000 abstract description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 8
- 239000000460 chlorine Substances 0.000 description 8
- 229910052801 chlorine Inorganic materials 0.000 description 8
- 239000000243 solution Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000005416 organic matter Substances 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 238000004062 sedimentation Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000005273 aeration Methods 0.000 description 2
- 238000005660 chlorination reaction Methods 0.000 description 2
- 230000015271 coagulation Effects 0.000 description 2
- 238000005345 coagulation Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 238000009287 sand filtration Methods 0.000 description 2
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Description
【発明の詳細な説明】
Ａ、産業上の利用分野
本発明は、浄水に用いる硝化菌固定化方法と浄水処理方
法に関する。DETAILED DESCRIPTION OF THE INVENTION A. Field of Industrial Application The present invention relates to a method for immobilizing nitrifying bacteria and a water purification treatment method for use in water purification.
３９発明の概要
請求項（１）の発明は、選択培養した硝化菌を濃縮した
液に粉末活性炭を添加し、撹拌しながら高吸性樹脂を添
加して膨潤したビーズに塩化カルシウムを添加して２次
架橋することにより硝化菌を固定するようにしたもので
ある。39 Summary of the Invention The invention of claim (1) is based on the method of adding powdered activated carbon to a concentrated solution of selectively cultured nitrifying bacteria, adding a superabsorbent resin while stirring, and adding calcium chloride to the swollen beads. Nitrifying bacteria are immobilized by secondary crosslinking.
請求項（２）の発明は、急速濾過システムの前段に請求
項（１）の方法で得た固定化硝化菌を用いた固定化硝化
処理装置を設けて、アンモニアを硝化して後、急速濾過
するものである。The invention of claim (2) provides a fixed nitrification treatment device using the fixed nitrifying bacteria obtained by the method of claim (1) upstream of the rapid filtration system, and after nitrifying ammonia, performs rapid filtration. It is something to do.
Ｃ１従来の技術
わが国においては、水道の需要は年々増加しているが、
供給能力には限界があり、地下水のような良質の水源が
得がたくなってきている。そのため、河川の表流水を水
源とすることが多くなっており、異臭味やトリハロメタ
ン生成などの問題を生じている。一方、美味な水に対す
る国民のニーズが高まっており、現在より高度な浄水処
理が要求されている。既存の浄水処理方式で主流を占め
ている急速濾過システムは水中の濁質の除去と殺菌が主
体であって溶解物質の除去にはあまり有効ではない。異
臭味やトリハロメタンは溶解性有機物に由来するもので
あり、これらを減少させるためには溶解性有機物の除去
能力を高める必要かある。C1 Conventional technology In Japan, the demand for water supply is increasing year by year.
Supply capacity is limited, and high-quality water sources such as groundwater are becoming difficult to obtain. For this reason, surface water from rivers is increasingly used as a water source, causing problems such as off-flavors and trihalomethane formation. On the other hand, the public's need for delicious water is increasing, and more advanced water purification treatment is currently required. Rapid filtration systems, which are the mainstream of existing water purification methods, mainly remove suspended matter and sterilize water, and are not very effective in removing dissolved substances. Off-flavors and trihalomethanes are derived from soluble organic substances, and in order to reduce these, it is necessary to increase the ability to remove soluble organic substances.
トリハロメタンはアンモニアの除去と殺菌を目的とした
塩素処理の過程において有機物と塩素反応により生成す
るものであるが原水中のアンモニア濃度が高い場合、必
然的に塩素注入量が多くなり、トリハロメタンの生成量
が増加する。Trihalomethane is produced by the reaction of organic matter with chlorine during the chlorination process for the purpose of removing ammonia and sterilizing it. However, if the ammonia concentration in raw water is high, the amount of chlorine injected will inevitably increase, and the amount of trihalomethane produced will increase. increases.
このため、アンモニアや有機物濃度の高い原水に対して
は塩素注入の前段階において、これらを除去するプロセ
スとして生物活性炭処理や生物酸化処理が行われている
。For this reason, biological activated carbon treatment and biological oxidation treatment are performed on raw water with high concentrations of ammonia and organic matter as a process to remove them before chlorine injection.
Ｄ０発明が解決しようとする課題
ところが、生物酸化処理は、冬季水温低下時に、アンモ
ニアを硝化する作用のある硝化菌が独立栄養菌であって
増殖速度が小さく、低温下で硝化活性が低下するため、
アンモニアを硝化する能力が低下する。Problems to be solved by the D0 invention However, in biological oxidation treatment, when the water temperature drops in winter, the nitrifying bacteria that have the effect of nitrifying ammonia are autotrophic bacteria, have a slow growth rate, and their nitrification activity decreases at low temperatures. ,
The ability to nitrify ammonia is reduced.
本発明は、従来の技術の有するこのような問題点に鑑み
てなされたものであり、その目的とするところは、アン
モニアの硝化能力が向上するように高濃度の硝化菌を固
定化する硝化菌固定化方法と、この方法で得た固定化硝
化菌を用いた固定化硝化装置によりアンモニアの硝化能
力を高め、硝化処理後、急速濾過するようにして、急速
濾過における塩素投入量を減少させることのできる浄水
処理方法を提供することにある。The present invention has been made in view of the above-mentioned problems of the conventional technology, and its purpose is to immobilize nitrifying bacteria at a high concentration so as to improve the nitrifying ability of ammonia. To increase the nitrification ability of ammonia by using an immobilization method and an immobilization nitrification device using immobilized nitrifying bacteria obtained by this method, and to perform rapid filtration after nitrification treatment, thereby reducing the amount of chlorine input in rapid filtration. The purpose of this invention is to provide a water purification method that allows for
８１課題を解決するための手段
上記目的を達成するために、本発明の硝化菌固定化方法
は、活性汚泥を選択培養した硝化菌を渦縮し、この濃縮
液に粉末活性炭を添加して撹拌しながら高吸水性樹脂を
添加して膨澗したビーズとし、このビーズに塩化カルシ
ウムを添加して２次架橋することにより硝化菌を固定化
するものである。81 Means for Solving the Problems In order to achieve the above object, the method for immobilizing nitrifying bacteria of the present invention involves vortexing activated sludge with selectively cultured nitrifying bacteria, adding powdered activated carbon to this concentrate, and stirring. Meanwhile, a super absorbent resin is added to form swollen beads, and calcium chloride is added to the beads for secondary crosslinking, thereby immobilizing nitrifying bacteria.
また、浄水処理方法は、急速濾過装置の前段にアンモニ
アを硝化する請求項（１）の硝化菌固定化方法にて得た
固定化硝化菌を用いた固定化硝化処理装置を設け、原水
を硝化処理後、急速濾過を行うにうよしたものである。In addition, the water purification treatment method includes installing an immobilized nitrification treatment device using immobilized nitrifying bacteria obtained by the nitrifying bacteria immobilization method of claim (1) for nitrifying ammonia in the front stage of the rapid filtration device, and nitrifying raw water. After treatment, rapid filtration is recommended.
Ｆ１作用
硝化菌固定化方法
活性汚泥を硝化菌培地で選択培養し、遠心分離又は自然
沈降させることにより濃縮液を得る。この濃縮液に活性
炭添加し、撹拌しながら高吸水性樹脂を添加することに
より膨澗したビーズを得る。Method for immobilizing F1-acting nitrifying bacteria Activated sludge is selectively cultured in a nitrifying bacteria medium, and a concentrated solution is obtained by centrifugation or natural sedimentation. Activated carbon is added to this concentrated solution, and a superabsorbent resin is added while stirring to obtain swollen beads.
これに塩化カルシウムを添加することにより２次架橋の
ビーズ、即ち高濃度の硝化菌を固定化した固定化硝化菌
を得ることができる。By adding calcium chloride to this, it is possible to obtain secondary crosslinked beads, that is, immobilized nitrifying bacteria in which a high concentration of nitrifying bacteria is immobilized.
浄水処理方法
急速濾過装置の前段に硝化菌固定化方法で得た高１度の
固定化硝化菌を用いた固定化硝化処理装置を設け、高濃
度の固定化硝化菌によりアンモニアの硝化処理を行うの
で、硝化能力が向上し、低温でもアンモニアを硝化処理
することができる急速濾過装置はアンモニアが硝化され
た原水を浄水処理するため、塩素の投入量を少なくする
ことができる。このため、人体に有害なトリハロメタン
の発生が減少する。Water purification treatment method A fixed nitrification treatment device using high-1 degree fixed nitrifying bacteria obtained by the nitrifying bacteria immobilization method is installed in front of the rapid filtration device, and ammonia nitrification is performed using a high concentration of immobilized nitrifying bacteria. Therefore, a rapid filtration device that has improved nitrification ability and can nitrify ammonia even at low temperatures purifies raw water in which ammonia has been nitrified, making it possible to reduce the amount of chlorine input. Therefore, the generation of trihalomethane, which is harmful to the human body, is reduced.
Ｇ、実施例 本発明の実施例について図面を参照して説明する。G. Example Embodiments of the present invention will be described with reference to the drawings.
先ず、第１図において、硝化菌の高濃化と固定化の方法
を説明する。First, referring to FIG. 1, a method for highly concentrating and immobilizing nitrifying bacteria will be explained.
上水処理場の活性汚泥１を種菌として硫化アンモニウム
を主体とする硝化菌用培地で選択培養２を行い、培養液
を遠心分離又は自然沈降して上澄液５を排除して硝化菌
濃縮液４を得る。この濃縮液１ｅに対して粉末活性炭適
量添加６して撹拌を行いつつ高吸水性樹脂（商品名アラ
ソーブ）１０〜２０ｇを添加７して膨潤したビーズとす
る。高吸水性樹脂は水を吸収して膨潤し、膨潤後に外力
を加えても水を放出しに（い性質を有しているので、硝
化菌は活性炭と共に膨潤した樹脂内に保持される。次に
塩化カルシウム２ｇを添加して約２０分撹拌を行い、２
次架橋することによりビーズの保水能力を高める。最後
に水洗９をしてから、ビーズを集めてｌＯ上水道原水の
生物処理用固定化硝化菌とする。Selective culture 2 is performed using activated sludge 1 from a water treatment plant as a seed culture in a nitrifying bacteria medium mainly containing ammonium sulfide, and the culture solution is centrifuged or allowed to settle naturally to remove the supernatant 5 to obtain a nitrifying bacteria concentrate. Get 4. To this concentrated liquid 1e, an appropriate amount of powdered activated carbon is added 6, and while stirring, 10 to 20 g of a super absorbent resin (trade name Arasorb) is added 7 to form swollen beads. Super absorbent resin absorbs water and swells, and after swelling, it does not release water even when external force is applied. Therefore, nitrifying bacteria are retained in the swollen resin together with activated carbon.Next Add 2g of calcium chloride to the solution and stir for about 20 minutes.
The water retention capacity of the beads is increased by subsequent crosslinking. Finally, after washing with water 9, the beads are collected and used as immobilized nitrifying bacteria for biological treatment of lO water supply raw water.
次に、第２図について、上水処理方法について説明する
。Next, the water treatment method will be explained with reference to FIG.
第２図において、１１はエアレージジン装置を備えた上
記固定化硝化菌を詰めた固定化硝化処理装置、１２はエ
アレージジン装置を備えた固定床式生物酸化処理装置、
１３は凝集沈澱、砂濾過。In FIG. 2, 11 is an immobilized nitrification treatment device packed with the above-mentioned immobilized nitrifying bacteria, and 12 is a fixed bed biological oxidation treatment device equipped with an aeration gin device.
13 is coagulation sedimentation and sand filtration.
塩素処理を行う急速濾過システムである。This is a rapid filtration system that performs chlorine treatment.
第２図（イ）は固定化硝化処理装置１１でエアレージジ
ンを行って原水中のアンモニアを亜硝酸性窒素及び硝酸
性窒素に変換し、タンク内の越流堰をオーバーフローし
た硝化処理した水を、既存の急速濾過システム１３で凝
集沈澱、砂濾過、塩素処理を行うものである。Figure 2 (A) shows that the immobilized nitrification treatment equipment 11 performs aeration to convert ammonia in the raw water into nitrite nitrogen and nitrate nitrogen, and the nitrified water that overflows the overflow weir in the tank is The existing rapid filtration system 13 performs coagulation sedimentation, sand filtration, and chlorination.
第２図（ロ）は原水の有機物濃度が高い場合に、固定化
処理装置１１の前段に固定床式生物酸化処理装置！２を
設けてエアレージジンを行い原水中の有機物を除去した
後に上記（イ）の固定硝化処理装置１１．急速濾過シス
テム１３による処理を行うものである。Figure 2 (b) shows a fixed-bed biological oxidation treatment device installed before the immobilization treatment device 11 when the raw water has a high organic matter concentration. After removing organic matter from the raw water by performing aerage gin using the fixed nitrification treatment equipment 11 of (a) above. Processing is performed by a rapid filtration system 13.
Ｈ８発明の効果
本発明は、上述のとおり構成されているので次に記載す
る効果を奏する。H8 Effects of the Invention Since the present invention is configured as described above, it produces the following effects.
■硝化菌固定化法においては、高濃度の硝化菌を固定化
することができる。■In the nitrifying bacteria immobilization method, high concentrations of nitrifying bacteria can be immobilized.
■浄化処理方法においては、高濃度の硝化菌を固定化し
た固定化硝化菌で原水中のアンモニアを硝化処理するの
で、硝化能力が向上し、従来不可能であった低温におけ
るアンモニアの処理が可能になる。■In the purification treatment method, ammonia in raw water is nitrified using immobilized nitrifying bacteria with a high concentration of nitrifying bacteria, improving nitrification ability and making it possible to process ammonia at low temperatures, which was previously impossible. become.
■低温下でも硝化能力があるので、硝化処理後における
急速濾過装置の塩素投入量を減少させることができる。■Since it has nitrification ability even at low temperatures, it is possible to reduce the amount of chlorine input into the rapid filtration equipment after nitrification treatment.
また塩素投入の減少により健康に有害なトリハロメタン
の生成を減少させることができる。Reducing chlorine input also reduces the production of trihalomethanes, which are harmful to health.
第１図は、本発明の硝化菌固定化方法の実施例を示す工
程図、第２図（イ）、（ロ）は夫々本発明の浄水処理方
法の実施例を示す浄水処理装置の概略説明図である。
外２名
第２図FIG. 1 is a process diagram showing an example of the method for immobilizing nitrifying bacteria of the present invention, and FIGS. 2 (a) and (b) are schematic explanations of water purification equipment showing examples of the water purification method of the present invention, respectively. It is a diagram. 2 people outside Figure 2
Claims (2)
縮液に粉末活性炭を添加して撹拌しながら高吸水性樹脂
を添加して膨潤したビーズとし、このビーズに塩化カル
シウムを添加して２次架橋することにより硝化菌を固定
化することを特徴とする硝化菌固定化方法。(1) Activated sludge is selectively cultured to concentrate nitrifying bacteria, powdered activated carbon is added to this concentrated solution, and while stirring, a super absorbent resin is added to form swollen beads, and calcium chloride is added to these beads. A method for immobilizing nitrifying bacteria, which comprises immobilizing nitrifying bacteria by secondary crosslinking.
項（１）の硝化菌固定化方法にて得た固定化硝化菌を用
いた固定化硝化処理装置を設け、原水を硝化処理後、急
速濾過することを特徴とする浄水処理方法。(2) An immobilized nitrification treatment device using the immobilized nitrifying bacteria obtained by the nitrifying bacteria immobilization method of claim (1) is installed before the rapid filtration device to nitrify ammonia, and after the raw water is nitrified, the A water purification method characterized by filtration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10133489A JPH02280893A (en) | 1989-04-20 | 1989-04-20 | Method for immobilizing nitrifying bacteria and water purifying treatment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10133489A JPH02280893A (en) | 1989-04-20 | 1989-04-20 | Method for immobilizing nitrifying bacteria and water purifying treatment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH02280893A true JPH02280893A (en) | 1990-11-16 |
Family
ID=14297938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10133489A Pending JPH02280893A (en) | 1989-04-20 | 1989-04-20 | Method for immobilizing nitrifying bacteria and water purifying treatment |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02280893A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04281894A (en) * | 1991-03-09 | 1992-10-07 | Central Res Inst Of Electric Power Ind | Sea water purifying material and sea water purifying method |
| JPH0780282A (en) * | 1993-06-24 | 1995-03-28 | Ebara Res Co Ltd | Polymeric hydrogel granular material, production thereof and immobilization of bacteria using the same |
| JP2003235554A (en) * | 2002-02-18 | 2003-08-26 | Hitachi Plant Eng & Constr Co Ltd | Microorganism-immobilized carrier and method for producing the same |
| JPWO2020004662A1 (en) * | 2018-06-29 | 2020-12-17 | 株式会社クラレ | Water treatment method |
| CN112409635A (en) * | 2020-10-30 | 2021-02-26 | 东华大学 | Method for separating, recycling and utilizing waste polyester-cotton blended fabric |
-
1989
- 1989-04-20 JP JP10133489A patent/JPH02280893A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04281894A (en) * | 1991-03-09 | 1992-10-07 | Central Res Inst Of Electric Power Ind | Sea water purifying material and sea water purifying method |
| JPH0780282A (en) * | 1993-06-24 | 1995-03-28 | Ebara Res Co Ltd | Polymeric hydrogel granular material, production thereof and immobilization of bacteria using the same |
| JP2003235554A (en) * | 2002-02-18 | 2003-08-26 | Hitachi Plant Eng & Constr Co Ltd | Microorganism-immobilized carrier and method for producing the same |
| JPWO2020004662A1 (en) * | 2018-06-29 | 2020-12-17 | 株式会社クラレ | Water treatment method |
| CN112409635A (en) * | 2020-10-30 | 2021-02-26 | 东华大学 | Method for separating, recycling and utilizing waste polyester-cotton blended fabric |
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