JPH02254346A - Reagent injector for emission measuring apparatus - Google Patents
Reagent injector for emission measuring apparatusInfo
- Publication number
- JPH02254346A JPH02254346A JP7753089A JP7753089A JPH02254346A JP H02254346 A JPH02254346 A JP H02254346A JP 7753089 A JP7753089 A JP 7753089A JP 7753089 A JP7753089 A JP 7753089A JP H02254346 A JPH02254346 A JP H02254346A
- Authority
- JP
- Japan
- Prior art keywords
- reagent
- injection tube
- injection
- injection port
- luminescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 52
- 238000002347 injection Methods 0.000 claims abstract description 81
- 239000007924 injection Substances 0.000 claims abstract description 81
- 238000005259 measurement Methods 0.000 claims abstract description 22
- 238000004020 luminiscence type Methods 0.000 claims description 36
- 239000000758 substrate Substances 0.000 claims description 5
- 230000000903 blocking effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 4
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- 239000007788 liquid Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 7
- 239000000427 antigen Substances 0.000 description 6
- 102000036639 antigens Human genes 0.000 description 6
- 108091007433 antigens Proteins 0.000 description 6
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000003018 immunoassay Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 238000005415 bioluminescence Methods 0.000 description 2
- 230000029918 bioluminescence Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は生物発光、化学発光の測定を行なう発光測定装
置用試薬注入器に関するものである。DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention relates to a reagent injector for a luminescence measuring device that measures bioluminescence and chemiluminescence.
（従来の技術）
医学、薬学、生化学等の分野においては、超微量物質の
分析に生物発光や化学発光による発光測定装置が用いら
れている。特に、酵素免疫測定法（エンザイム・イムノ
アッセイ）における酵素標識抗体の検出には、高感度で
取扱いが容易であるこの発光測定装置が最適である。(Prior Art) In the fields of medicine, pharmacy, biochemistry, etc., luminescence measurement devices using bioluminescence or chemiluminescence are used to analyze ultratrace substances. In particular, this luminescence measuring device, which is highly sensitive and easy to handle, is optimal for detecting enzyme-labeled antibodies in enzyme immunoassays.
前記酵素免疫測定法は、予め試料台である酵素免疫測定
用プレートの有底試料孔に、被測定対象物に対する特異
抗体を結合させておき（抗体の固相化）、これに被測定
対象物である抗原を含む血液等の試料液を加えて免疫反
応（抗原抗体反応）を行なわせ、抗原を固相化抗体に結
合させる。次に、未反応物を洗浄して除去した後、固相
化抗体に結合した抗原に、さらに酵素標識抗体を加えて
結合させ、この抗原に結合した酵素標識抗体の量を測定
することにより、被測定対象物である抗原の量を求める
方法である。In the enzyme immunoassay method, a specific antibody against the object to be measured is bound in advance to the bottomed sample hole of the enzyme immunoassay plate, which is a sample stage (immobilization of the antibody), and then the object to be measured is attached to this. A sample liquid such as blood containing a certain antigen is added to cause an immune reaction (antigen-antibody reaction), and the antigen is bound to the immobilized antibody. Next, after washing and removing unreacted substances, an enzyme-labeled antibody is further added and bound to the antigen bound to the immobilized antibody, and the amount of enzyme-labeled antibody bound to this antigen is measured. This is a method for determining the amount of antigen, which is the object to be measured.
このような酵素免疫測定法における酵素標識抗体の検出
に最適な発光測定装置は、一般に試薬注大器と計測器と
を備えている。試薬注入器は、第９図に示すように、測
定用プレートｌの有底試料孔２内で固相化抗体に結合し
た被測定対象物、すなわち抗原に、さらに、特異的な酵
素標識抗体を結合させた被測定試料３に、発光基質であ
るルミノールや酸化剤である過酸化水素等の発光試薬４
を針状の注入管５を介して注入するものである。A luminescence measuring device most suitable for detecting an enzyme-labeled antibody in such an enzyme immunoassay method is generally equipped with a reagent pouring device and a measuring device. As shown in FIG. 9, the reagent injector further injects a specific enzyme-labeled antibody into the analyte, that is, the antigen, bound to the immobilized antibody in the bottomed sample hole 2 of the measurement plate L. A luminescent reagent 4 such as luminol, which is a luminescent substrate, and hydrogen peroxide, which is an oxidizing agent, is added to the bound sample to be measured 3.
is injected through a needle-shaped injection tube 5.
また、計測器は、測定用プレートｌの試料孔２の下方に
配置した受光部６にて、試料孔２内で化学発光反応によ
り発する光を受光し、その発光量を光子計数法で測定す
るものである。In addition, the measuring instrument receives light emitted by a chemiluminescent reaction within the sample hole 2 with a light receiving section 6 placed below the sample hole 2 of the measurement plate l, and measures the amount of light emitted by a photon counting method. It is something.
この発光測定装置による発光測定は、外部光があると正
確な測定が行なえないため、通常遮光を十分に施した暗
箱の中で行なわれる。また、発光反応は極めて短時間に
行なわれ、試薬を注入すると同時に終了するものである
ため、測定スイッチと連動した試薬の自動注入器が使用
されている。Luminescence measurements using this luminescence measuring device are usually carried out in a dark box that is sufficiently shielded from light, since accurate measurements cannot be made in the presence of external light. Furthermore, since the luminescent reaction occurs in an extremely short time and ends at the same time as the reagent is injected, an automatic reagent injector that is linked to a measurement switch is used.
（発明が解決しようとする課題）
しかしながら、ルミノールと過酸化水素からなる試薬４
はそれ自体幾分か発光を帯びていることから、計測器の
受光部６は、本来測定すべき試料孔２内の被測定試料３
の発光以外に、注入管５の内部の残存試薬４が帯びる発
光を計測してしまい、測定精度に悪影響を及ぼすという
問題があった。(Problem to be solved by the invention) However, reagent 4 consisting of luminol and hydrogen peroxide
Since the light emitting element itself has some luminescence, the light receiving part 6 of the measuring instrument detects the sample to be measured 3 in the sample hole 2 to be measured.
In addition to the luminescence of the residual reagent 4 inside the injection tube 5, the luminescence of the remaining reagent 4 inside the injection tube 5 is measured, which has a negative effect on measurement accuracy.
また、従来の発光測定装置の試薬注入器の注入管５は、
第９図に示すように、先端が斜め若しくは垂直に切断さ
れ１こ針状等に形成されているため、液ぎれが悪く、試
薬注入後に注入ポンプの残圧等の原因によって先端に残
漬４ａが生成される。この結果、この残溜４ａ自体の発
光らまた受光部６で計測されることになり、さらに測定
精度が悪くなるという問題があった。In addition, the injection tube 5 of the reagent injector of the conventional luminescence measuring device is
As shown in Fig. 9, since the tip is cut diagonally or vertically and formed into a single needle shape, the liquid does not easily drip, and after injecting the reagent, residual pressure in the injection pump may cause residual pressure (4a) at the tip. is generated. As a result, the light emission of the residual reservoir 4a itself is also measured by the light receiving section 6, which further deteriorates the measurement accuracy.
本発明は斯かる問題点に鑑みてなされたもので、注入管
の残存試薬又は残漬の発光が試料の発光量測定に影響を
与えることのない発光測定装置用試薬注入器を提供する
ことを目的とする。The present invention has been made in view of such problems, and it is an object of the present invention to provide a reagent injector for a luminescence measuring device in which residual reagent in the injection tube or residual luminescence does not affect the measurement of the luminescence amount of the sample. purpose.
（課題を解決するための手段）
前記目的を達成するため、本第１発明は、注入管を介し
て被測定試料に発光基質からなる試薬を注入する発光測
定装置用試薬注入器において、注入管の内径をその先端
の注入口より拡径するとともに、注入管の注入口近傍に
屈曲部を形成したものである。(Means for Solving the Problems) In order to achieve the above object, the first invention provides a reagent injector for a luminescence measuring device that injects a reagent made of a luminescent substrate into a sample to be measured through an injection tube. The inner diameter of the injection tube is increased from the injection port at its tip, and a bent portion is formed near the injection port of the injection tube.
また、第２発明は、注入管を介して被測定試料に発光基
質からなる試薬を注入する発光測定装置用試薬注入器に
おいて、注入管の内径をその先端の注入口より拡径する
とともに、注入口より漏出しようとする試薬の光を遮光
する遮光板を注入管内部に装着したものである。A second aspect of the invention is a reagent injector for a luminescence measuring device that injects a reagent made of a luminescent substrate into a sample to be measured through an injection tube, in which the inner diameter of the injection tube is expanded from the injection port at its tip, and A light-shielding plate is installed inside the injection tube to block light from the reagent leaking from the inlet.
（作用）
前記第１発明の構成によれば、注入管の注入口近傍に形
成した屈曲部により、注入口から軸方向の直線部分が短
くなり、注入管内の残存試薬の発光の漏出量が減少する
。また、拡径した注入管より注入口に至るまで断面積が
縮小して圧力損失が生じることにより、注入器の残圧が
減圧されるうえ、注入口の開口面積が小さいことにより
、残圧と重力とによる滴下しようとする力が減少する結
果、液ぎれがよく、残漬は生成されない。(Function) According to the configuration of the first invention, the bent portion formed near the injection port of the injection tube shortens the straight portion in the axial direction from the injection port, reducing the amount of leakage of light emitted from the remaining reagent in the injection tube. do. In addition, the cross-sectional area from the expanded diameter injection pipe to the injection port decreases, causing pressure loss, which reduces the residual pressure in the injection device, and the small opening area of the injection port reduces the residual pressure. As a result of the reduction in the force exerted by gravity to cause the liquid to drip, the liquid drains easily and no residual liquid is generated.
一方、第２発明に係る構成によれば、遮光板により当該
遮光板より上流側の試薬の発光が遮光されるため、注入
口からの試薬の発光の漏出量が減少する。また、この第
２発明においても注出管内径が注入口より拡径されてい
るため、前記第１発明と同様、残漬は生成されない。On the other hand, according to the configuration according to the second aspect of the invention, since the light shielding plate blocks the light emitted from the reagent upstream of the light shielding plate, the amount of light emitted from the reagent leaking from the injection port is reduced. Further, in the second invention as well, since the inner diameter of the pouring pipe is larger than the inlet, no residual liquid is generated as in the first invention.
（実施例） 次に、本発明の実施例を添付図面に従って説明する。(Example) Next, embodiments of the present invention will be described with reference to the accompanying drawings.
ｉ）第１発明の実施例
第１図は本第１発明に係る試薬注入器の注入管１０を示
し、この注入管１０は、酸、アルカリに対する耐食性が
良く、しかも液ぎれのよい疎水性を宵するうえ、光の漏
出のない高密度の材料、具体的にはステンレス鋼、チタ
ン等の材料で形成されている。i) Embodiment of the first invention FIG. 1 shows an injection tube 10 of a reagent injector according to the first invention. They are made of high-density materials, specifically materials such as stainless steel and titanium, that are light-emitting and do not allow light to leak through.
そして、注入管ｌＯの先端部は、円錐状に形成され、注
入口１１の近傍の内径ｄ、が注入口の内径ｄはり拡径さ
れている。また、注入口１１の近傍には、屈曲部１２が
形成され、さらに該屈曲部１２より上方は、第２図に示
すように、コの字形に屈曲されている。なお、屈曲部１
２より上方の形状は、第３図、第４図に示す注入管１０
ａ、１０ｂのように、ループ形、あるいはダブルループ
形等にしてもよい。The tip of the injection tube IO is formed into a conical shape, and the inner diameter d near the injection port 11 is larger than the inner diameter d of the injection port. Further, a bent portion 12 is formed near the injection port 11, and the portion above the bent portion 12 is bent into a U-shape as shown in FIG. In addition, the bent part 1
The shape above 2 is the injection pipe 10 shown in FIGS. 3 and 4.
It may be a loop shape or a double loop shape as shown in a and 10b.
注入管１０に屈曲部１２が形成されることにより、注入
口１１から軸方向の直線部の長さしが従来の注入管より
短くなる。したがって、注入管１０内の発光を帯びた試
薬４から注入口１１を介して漏出する光は、第１図中−
点鎖線で囲む範囲内のものとなり、漏出量が減少する結
果、受光部６での被測定試料３の発光量の測定（第９図
参照）に与える影響が少なくなる。By forming the bent portion 12 in the injection tube 10, the length of the straight portion in the axial direction from the injection port 11 becomes shorter than that of a conventional injection tube. Therefore, the light leaking from the luminescent reagent 4 in the injection tube 10 through the injection port 11 is -
As a result, the amount of leakage is reduced and the influence on the measurement of the amount of light emitted from the sample 3 to be measured (see FIG. 9) at the light receiving section 6 is reduced.
また、拡径した注入管１０より注入口１１へは断面積が
縮小して圧力損失が生じるため、試薬４の残圧は注入口
１１の位置で減圧され、当該試薬４を流出させるのに必
要な圧力には達しない。In addition, since the cross-sectional area from the enlarged injection pipe 10 to the injection port 11 is reduced and a pressure loss occurs, the residual pressure of the reagent 4 is reduced at the injection port 11, which is necessary for the reagent 4 to flow out. pressure cannot be reached.
さらに、注入口１１の開口面積が小さく、試薬４の残圧
と重力とにより滴下しようとする力が減少する。そのう
え、注入管１０は疎水性のある材料で形成されているこ
とから、液ぎれが良く、第９図に示すような残層は生成
されない。Furthermore, the opening area of the injection port 11 is small, and the force of dripping due to the residual pressure of the reagent 4 and gravity is reduced. Moreover, since the injection tube 10 is made of a hydrophobic material, the liquid drains easily and no residual layer as shown in FIG. 9 is generated.
）第２発明の実施例
第５図は第２発明に係る試薬注入器の注入管２０を示し
、この注入管２０は、その内部に遮光板２１を装着した
以外は前記第１発明に係る注入管ＩＯと実質的に同一で
あり、対応する部分には同一符号を付して説明を省略す
る。) Embodiment of the second invention FIG. 5 shows an injection tube 20 of a reagent injector according to the second invention. It is substantially the same as the pipe IO, and corresponding parts are denoted by the same reference numerals and description thereof will be omitted.
遮光板２１は、注入管２０の内面に接する円板で、その
中心を囲むように複数の流通孔２２を穿設したものであ
る。なお、本第２発明は遮光板２１が注入管２０内の試
薬４の発光の漏出量を制限するため、屈曲部１２は必ず
しも必要ない。The light shielding plate 21 is a circular plate that is in contact with the inner surface of the injection tube 20, and has a plurality of communication holes 22 formed around the center thereof. In addition, in the second invention, since the light shielding plate 21 limits the amount of light emitted from the reagent 4 inside the injection tube 20, the bent portion 12 is not necessarily required.
この遮光板２１を装着した注入管２０では、当該遮光板
２１より上流側の試薬４の発光が遮光される。したがっ
て、注入管２０内の発光を帯びた試薬４より注入口１１
を介して漏出する光は、遮光板２１より下流側の第５図
中−点鎖線で囲む範囲内のものとなり、漏出量が減少す
る結果、受光部６での被測定試料３の発光量の測定（第
９図参照）に与える影響が少なくなる。In the injection tube 20 equipped with this light shielding plate 21, light emission from the reagent 4 on the upstream side of the light shielding plate 21 is blocked. Therefore, the luminescent reagent 4 in the injection tube 20 is transferred to the injection port 11.
The light leaking through the light shielding plate 21 falls within the range surrounded by the dotted chain line in FIG. The influence on measurements (see Figure 9) is reduced.
また、本第２発明においても、注入管２０の内径が注入
口１１より拡径されているため、液ぎれが良く、残層が
生成されないことは、前記第１発明と同様である。Also, in the second invention, since the inner diameter of the injection tube 20 is larger than the injection port 11, liquid drips well and no residual layer is generated, as in the first invention.
ところで、遮光板２１には流通孔２２が穿設されている
ため、試薬４の注入時に試薬４の流れが妨げられること
はない。By the way, since the light shielding plate 21 is provided with the flow holes 22, the flow of the reagent 4 is not obstructed when the reagent 4 is injected.
１１１）確認実験
本発明者らは、本発明に係る試薬注入器の有効性を確認
するために以下の実験を行なった。111) Confirmation Experiment The present inventors conducted the following experiment to confirm the effectiveness of the reagent injector according to the present invention.
実験１．拡径の有効性
拡径した注入管を有する試薬注入器（ただし、第１図に
示すような屈曲部分は有していない。）を備えた発光測
定装置Ａと、第９図に示す従来の発光測定装置Ｂとを用
いである被測定試料の発光測定を行ない、それぞれの発
光量を光電子パルス数の時間的変化で計測した。Experiment 1. Effectiveness of Diameter Expansion A luminescence measuring device A equipped with a reagent injector having an expanded diameter injection tube (however, it does not have a bent part as shown in FIG. 1) and a conventional luminescence measurement device A shown in FIG. Luminescence measurement of a sample to be measured was performed using luminescence measuring device B, and the amount of each luminescence was measured by the temporal change in the number of photoelectron pulses.
第７図はこの実験結果を示し、これによれば、試薬を被
測定試料に注入すると発光反応が行なわれて発光量がピ
ークに達し、その後従来の装置Ｂでは発光量が時間とと
もに増大し、装置Ａでは横ばいになっている。これは、
従来の装置Ｂでは、試薬注入後に注入管の先端に試薬の
残層が生成され、これが時間とともに大きく成長してゆ
く結果、その残層の発光が受光部に計測されたことを示
している。これに対し、装置Ａでは、この上うな残層の
生成がないため、発光量が変化しないことがわかる。FIG. 7 shows the results of this experiment, which show that when the reagent is injected into the sample to be measured, a luminescence reaction occurs and the luminescence amount reaches a peak, and then in the conventional device B, the luminescence amount increases with time. In device A, it remains flat. this is,
In conventional device B, a residual layer of the reagent is generated at the tip of the injection tube after the reagent is injected, and as a result, this layer grows larger over time, indicating that the light emission of the residual layer is measured by the light receiving section. On the other hand, it can be seen that in device A, since no residual layer is generated, the amount of light emission does not change.
実験２．屈曲部、遮光板の有効性
第１図に示す本発明に係る試薬注入器を備えた発光測定
装置Ａ１と、第５図に示す第２発明に係る試薬注入器を
備えた発光測定装置Ａ、と、拡径したままで屈曲部を有
しない注入管を備えた発光測定装置ＢＩとを用いである
被測定試料の発光測定を行ない、前記実験１と同様、発
光量の時間的変化を計測した。Experiment 2. Effectiveness of bending portion and light shielding plate A luminescence measurement device A1 equipped with a reagent injector according to the present invention shown in FIG. 1, a luminescence measurement device A equipped with a reagent injector according to the second invention shown in FIG. and a luminescence measurement device BI equipped with an injection tube with an enlarged diameter and no bent part.The luminescence measurement of a sample was performed, and as in Experiment 1, the temporal change in luminescence amount was measured. .
第８図はこの実験結果を示し、これによれば、ピークに
達した後の発光量は装置Ｂ３、装置Ａ１、装置Ａ、の順
で少なくなっている。これは、注入管内の発光を帯びた
試薬からの受光部への光の入射が、屈曲部を設けた装置
ＡＩの方が装置Ｂ、より少なく、また遮光板を設けた装
置Ａ、の方が装置Ｂ１及び装置Ａ、より少なく、注入管
内の試薬の発光の影響が少ないことを示している。FIG. 8 shows the results of this experiment, which show that the amount of light emitted after reaching the peak decreases in the order of device B3, device A1, and device A. This is because the incidence of light from the luminescent reagent in the injection tube into the light receiving section is lower in device AI, which has a bent section, than in device B, and is also lower in device A, which has a light shielding plate. Device B1 and device A are less, indicating less influence of the luminescence of the reagent in the injection tube.
（発明の効果）
以上の説明から明らかなように、本第１発明及び第２発
明によれば、いずれも、注入管内の試薬の発光の漏出最
が少なく、また残置の生成がないため、発光測定に与え
る影響が少なく正確な測定を行なうことができるという
効果を有している。(Effects of the Invention) As is clear from the above description, according to the first invention and the second invention, the leakage of luminescence from the reagent in the injection tube is minimal, and no residue is generated, so that luminescence is reduced. This has the effect that accurate measurements can be performed with little influence on measurements.
第１図〜第４図は本第１発明に係る注入器の注入管を示
し、第１図は注入管先端部の断面図、第２図は注入管の
屈曲形状を示す正面図、第３図。
第４図は他の屈曲形状を有する注入管の正面図、第５図
は本第２発明に係る注入器の注入管先端部の断面図、第
６図は遮光板の平面図、第７図は拡径の有効性に関する
実験結果を示す図、第８図は屈曲部、遮光板の有効性に
関する実験結果を示す図、第９図は従来の発光測定装置
の断面図である。
３・・・被測定試料、
１０・・・注入管、
１２・・・屈曲部、
２１・・遮光板。
４・・試薬、
１１・・・注入口、
・注入管、
特 許 出 願 人 帝國製薬株式会社代 理 人 弁
理士 青白 葆 ほか１名第１図
第５図
第７図
第６図
第８図
第２図
第３図
第４図1 to 4 show the injection tube of the syringe according to the first invention, FIG. 1 is a sectional view of the tip of the injection tube, FIG. 2 is a front view showing the bent shape of the injection tube, and FIG. figure. FIG. 4 is a front view of an injection tube having another bent shape, FIG. 5 is a sectional view of the tip of the injection tube of the syringe according to the second invention, FIG. 6 is a plan view of the light shielding plate, and FIG. 7 8 is a diagram showing the experimental results regarding the effectiveness of diameter expansion, FIG. 8 is a diagram showing the experimental results regarding the effectiveness of the bent portion and the light shielding plate, and FIG. 9 is a cross-sectional view of a conventional luminescence measuring device. 3... Sample to be measured, 10... Injection tube, 12... Bent part, 21... Light shielding plate. 4. Reagent, 11. Inlet port, ・Injection tube, Patent applicant: Teikoku Seiyaku Co., Ltd. Representative, Patent attorney: Aobai Ao and 1 other person Figure 1 Figure 5 Figure 7 Figure 6 Figure 8 Figure 2 Figure 3 Figure 4
Claims (2)
薬を注入する発光測定装置用試薬注入器において、注入
管の内径をその先端の注入口より拡径するとともに、注
入管の注入口近傍に屈曲部を形成したことを特徴とする
発光測定装置用試薬注入器。(1) In a reagent injector for a luminescence measuring device that injects a reagent consisting of a luminescent substrate into a sample to be measured through an injection tube, the inner diameter of the injection tube is expanded from the injection port at its tip, and A reagent injector for a luminescence measuring device, characterized in that a bent portion is formed in the vicinity.
薬を注入する発光測定装置用試薬注入器において、注入
管の内径をその先端の注入口より拡径するとともに、注
入口より漏出しようとする試薬の光を遮光する遮光板を
注入管内部に装着したことを特徴とする発光測定装置用
試薬注入器。(2) In a reagent injector for a luminescence measurement device that injects a reagent consisting of a luminescent substrate into a sample to be measured through an injection tube, the inner diameter of the injection tube is expanded from the injection port at its tip, and the leakage from the injection port is prevented. 1. A reagent injector for a luminescence measuring device, characterized in that a light shielding plate for blocking light from a reagent is installed inside the injection tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1077530A JP2786470B2 (en) | 1989-03-29 | 1989-03-29 | Reagent injector for luminescence measurement device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1077530A JP2786470B2 (en) | 1989-03-29 | 1989-03-29 | Reagent injector for luminescence measurement device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02254346A true JPH02254346A (en) | 1990-10-15 |
| JP2786470B2 JP2786470B2 (en) | 1998-08-13 |
Family
ID=13636538
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1077530A Expired - Fee Related JP2786470B2 (en) | 1989-03-29 | 1989-03-29 | Reagent injector for luminescence measurement device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2786470B2 (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63122960A (en) * | 1986-11-12 | 1988-05-26 | Toshiba Corp | Liquid dispending device |
| JPS63286770A (en) * | 1987-05-20 | 1988-11-24 | Toshiba Corp | Dispensing nozzle and fixed volume dispensing pump for liquid analyzer for medical inspection |
-
1989
- 1989-03-29 JP JP1077530A patent/JP2786470B2/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63122960A (en) * | 1986-11-12 | 1988-05-26 | Toshiba Corp | Liquid dispending device |
| JPS63286770A (en) * | 1987-05-20 | 1988-11-24 | Toshiba Corp | Dispensing nozzle and fixed volume dispensing pump for liquid analyzer for medical inspection |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2786470B2 (en) | 1998-08-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11931734B2 (en) | Lateral-flow assay device having flow constrictions | |
| US5156976A (en) | Evanescent wave sensor shell and apparatus | |
| AU639703B2 (en) | Automated method and device for performing solid-phase diagnostic assay | |
| US4585623A (en) | Device for performing quantitative chemical and immunochemical assays | |
| AU2004312893B2 (en) | Assay device and method | |
| US5006309A (en) | Immunoassay device with liquid transfer between wells by washing | |
| CA1231893A (en) | Binding assay system and method of making and using same | |
| CN1161602C (en) | Minimally invasive sensor | |
| WO2003100469A3 (en) | Internal biochemical sensing device | |
| JPH03154853A (en) | Chemical method and device for identifying dissimilar luminescence | |
| WO1994000761A1 (en) | Optical measurement instrument | |
| US8570518B2 (en) | Methods and materials for detection of target species | |
| JPH02236453A (en) | Detection of quantity of emitted light of immunoassay device | |
| CA2294020C (en) | Dual injector for chemiluminescence immunoanalyzing system | |
| JPH02254346A (en) | Reagent injector for emission measuring apparatus | |
| JP3121406B2 (en) | Luminescent sample test equipment | |
| JP4829460B2 (en) | Sealed container and screening method | |
| EP1965195A1 (en) | Luminescence measuring apparatus | |
| US4501497A (en) | Cell for measurement | |
| CN109917122B (en) | Chemiluminescence detection device | |
| JPH02254364A (en) | Plate for measuring immunity of biological/chemical luminescent enzyme | |
| KR102416982B1 (en) | Rapid kit comprising icrofluidic device with intermediate venthole | |
| JP3484180B2 (en) | Reaction vessel | |
| WO2022209396A1 (en) | Test device and cartridge | |
| JP3787302B2 (en) | Measuring chip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |