JPH02247563A - Analysis - Google Patents

Abstract

PURPOSE:To eliminate the influence of the specimen liquid previously spotted by adding a stage for treating multilayered analyzing elements in such a manner that the influence occurring in a gaseous product is substantially eliminated in the coloration reaction in the next spotting position. CONSTITUTION:A multilayered analyzing tape 1 is transported from a right side to a left side. The latest specimen liquid alpha0 is spotted to the spotting part A0 and the specimen liquids (alpha-1) to (alpha-n) are spotted to the spotting parts A-1 to A-n on the left side thereof. Photometry is already ended. The tape 1 is transported by one frame to bring the next spotting part A1 to the spotting position (a) when urine nitrogen is low in concn. as a result of the photometry of the specimen liquid alpha0. The specimen liquid alpha1 is then subjected to the photometry. The tape 1 is transported by 2 frames, i.e. in such a manner that the next spotting part A3 comes to the position (a) when the specimen liquid alpha1 contains the urine nitrogen of a high concn. as a result of the above- mentioned photometry. The high-concn. gaseous NH3 generated from the specimen liquid alpha1 is diffused and is lowered in the concn. before the tape comes to the spotting part A3 and, therefore, the exact photometry is executed with substantially no influence exerted on the specimen liquid alpha2.

Description

Detailed Description of the Invention [Industrial Application Field] The present invention uses a long tape-shaped multilayer analysis element (sometimes referred to as a multilayer analysis film) (hereinafter referred to as a multilayer analysis tape) to analyze biological body fluids. The present invention relates to a chemical and/or biochemical analysis method for quantifying specific components present in a sample.

[Conventional technology]

Dry multilayer analysis is used for quantitative analysis of various metabolic components present in body fluids, such as glucose, bilirubin, urea nitrogen (BUN), creatine, ammonia, uric acid, cholesterol, lactate dehydrogenase, talleatin kinase, GOT, and GPT. Increasingly, chemical analysis is carried out by a dry method using a chemical analysis slide in which the element is cut into small pieces and placed in a frame.

However, chemical analysis methods using chemical analysis slides require time and expense to manufacture the chemical analysis slides, and are not economically efficient.

Therefore, a chemical analysis method has been proposed in which a long tape-shaped multilayer analysis element is used instead of a chemical analysis slide, thereby saving the labor and cost of manufacturing a chemical analysis slide (U.S. Pat. No. 3,260,413). Specification of No. 3,526
．． 480 specification, Japanese Patent Publication No. 53-21677 (US Pat. No. 3,992.158), etc.).

As a chemical analyzer used in such a chemical analysis method, for example, the one shown in JP-A-63-313063 shown in FIG.
There is an apparatus described in the specification of No.

In FIG. 8, reference numeral 71 denotes a multilayer analytical tape, and this multilayer analytical tape 71 has a reel 7 for feeding out on one side.
2, and the receiver is stored in a box 74 via a winding roller 73 on the other hand.

An outer frame 75 is provided on the outer periphery of the second take-up roller 73, and a spotting hole 76 is provided at the tip of the outer frame 75 so that it can be opened and closed by a shutter 77 that can be opened and closed. This shutter 77
Below and above the multilayer analysis tape 71, there is provided an incubator 78 that can be raised and lowered vertically and moved forward and backward in the direction of movement of the multilayer analysis tape 71. optical measurement probe 79
The optical measuring probe 79 is provided with a photometric means 8.
0 and a light source 81 are connected by optical fibers 82 and 83.

Further, at the top, a spotting means 85 for spotting the test liquid onto the multilayer analysis tape 71 is moved in the horizontal direction so as to be freely positioned directly above the shutter 77.

Next, a process of analyzing a test liquid using the above analyzer will be explained.

First, with the multilayer analysis tape 71 stopped, the incubator 78 is moved to the winding side, and the shutter 7 is moved.
7 to expose the spotting part of the multilayer analysis tape 71.
(hole opening process).

Next, the spotting means 85 is moved horizontally to be positioned above the spotting hole 76 and then lowered to approach above the spotting section. Thereafter, a predetermined amount of the test liquid 86, for example 5J11, is applied (spotting step).

After removing the spotting means 85 from the outer frame 75, the shutter 77 is returned to close the spotting hole 76, and the incubator 78 is also returned and lowered to cover the spotting portion and perform incubation (incubation step). ).

After heating the spotting part in this state with the incubator 78,
Incident light is emitted from the light source 81, and the reflected light is measured by the photometry means 80 (photometry step).

Furthermore, after raising the incubator 78, the multilayer analysis tape 71 is fed one frame, and the next spotting part is placed in the spotting hole 76.
Position it below (conveyance process).

Thereafter, the same procedure is repeated, and the test liquid is sequentially applied to the application area and photometry is performed.

[Problem to be solved by the invention]

By the way, urea nitrogen (BUN) is among the test components of the test liquid measured with the multilayer analysis tape, and this urea nitrogen reacts with the reagent containing urase on the multilayer analysis tape to produce ammonia as a gaseous product. If the concentration of urea nitrogen is high, it will affect the next measurement of the test liquid, making it impossible to conduct an accurate test.

That is, the ammonia generated from the spotting section remains at the spotting position, and the next spotting section is where the test liquid is spotted and photometered in a high-concentration ammonia atmosphere.

In addition, the photometered spotting section is sent one frame toward the take-up roller 73, and a new spotting section is sent to the spotting position.
Even at this time, the reaction at the spotting part has not stopped and ammonia is generated, and this generated ammonia may affect the next spotting part.

Furthermore, since ammonia generated during incubation is once adsorbed to the incubator and then desorbed, the desorbed ammonia affects the photometry of the next spotting section.

However, the conventional analytical method using a multilayer analytical tape cannot be said to fully solve the above-mentioned problems.

The present invention aims to substantially eliminate the above-mentioned problems and to provide an analysis method that is hardly affected by the previously applied test liquid.

[Means to solve the problem]

The present invention has been made to achieve the above-mentioned object, and a first aspect of the present invention includes a conveying step for intermittently conveying a long integrated multilayer analytical element by a predetermined length, A spotting step in which the test liquid is spotted at a predetermined spotting site on the developed layer of the multilayer analytical element, and the spotting site on which the test liquid was spotted in the spotting process is ink-based. an ink washing step, direct or indirect interaction between the test component contained in the spotted test liquid after ink washing in the ink washing step and the reagent composition contained in the multilayer analysis element; In an analytical method that repeats a photometric process of measuring the optical density value of the color of the multilayer analytical element based on a color reaction, a step of determining the amount of gaseous products produced that affects the color reaction during the photometric process. includes a reaction that generates a gaseous product that affects the coloring reaction during the reaction between the components in the test liquid and the reagent composition, and the gaseous product When it is determined in the determination step that the test component is contained in the applied test liquid in an amount that produces a large amount of characterized by the addition of a step of treating the multilayer analytical element so that the influence caused by the gaseous product is substantially eliminated in the color reaction at the next predetermined spotting site of the element. It is configured.

The step of treating the multilayer analytical element so as to substantially eliminate the effects caused by gaseous products in the first aspect of the invention includes, for example, the following embodiments.

The first embodiment is a method of transporting a multilayer analytical element longer than a predetermined transport length.

In a second embodiment, before applying a different test liquid, a liquid capable of absorbing gaseous products generated from the test component is applied.
This is a method in which the test liquid is spotted on a predetermined site between the site where the test liquid was spotted and the next predetermined spot on the multilayer analysis element.

The third embodiment is a method in which a liquid capable of stopping the generation of gaseous products is applied to a region of a multilayer analysis element where the test liquid is applied.

A fourth embodiment is a method of substantially removing the gaseous products near the part of the multilayer analytical element where the test liquid is spotted using a gas flow that does not substantially contain the gaseous products. It is.

The fifth embodiment reduces the concentration of gaseous products in the atmosphere by improving ventilation near the part of the multilayer analysis element where the test liquid is spotted and diffusing the gaseous products. It's a method.

The sixth embodiment is a method for quickly sending out the site where the test liquid has been spotted after photometry.

A second invention provides a transport step of intermittently transporting a long integrated multilayer analytical element by predetermined lengths, A spotting step in which a test liquid is spotted on a spotting site; an incubation step in which the spotting site where the test liquid was spotted in the spotting step is placed inside an ink beta; Later, the optical density value of the color of the multilayer analysis element is measured based on a direct or indirect color reaction between the test component contained in the spotted test liquid and the reagent composition contained in the multilayer analysis element. In an analytical method that repeats a photometric process, when the multilayer analytical element contains a reagent composition that generates a gaseous product that affects the color reaction by reaction with the test component, the following The multilayer analysis element is designed such that the influence caused by the gaseous products is substantially eliminated in the color reaction at the next predetermined spotting site of the multilayer analytical element on which different test liquids are spotted and photometered. The method is characterized in that a step of processing analysis elements is added.

The process of treating the multilayer analytical element so as to substantially eliminate the influence caused by gaseous products in the second aspect of the invention includes, for example, the following embodiments.

The first embodiment is a method of transporting a multilayer analytical element longer than a predetermined transport length.

In a second embodiment, before applying a different test liquid, a liquid capable of absorbing gaseous products generated from the test component is applied.
This is a method in which the test liquid is spotted on a predetermined site between the site where the test liquid was spotted and the next predetermined spot on the multilayer analysis element.

The third embodiment is a method in which a liquid capable of stopping the generation of gaseous products is applied to a region of a multilayer analysis element where the test liquid is applied.

A fourth embodiment is a method of substantially removing the gaseous products near the part of the multilayer analytical element where the test liquid is spotted using a gas flow that does not substantially contain the gaseous products. It is.

The fifth embodiment is a method of diffusing gaseous products by improving the ventilation near the part of the multilayer analysis element where the test liquid is spotted.

The sixth embodiment is a method for quickly sending out the site where the test liquid has been spotted after photometry.

In the first aspect, the length of transporting the multilayer analysis tape longer than usual varies depending on the concentration of the test liquid, the amount of spotting, etc. When the length is 15 m, a length of approximately 20 mm or more is required.

In the second embodiment, the liquid capable of adsorbing gaseous products may be any solution that can be removed from the air by reacting with the gaseous products in the air. For example, in the case of ammonia gas, hydrochloric acid, sulfuric acid, phosphoric acid, etc. etc. or water. The concentration and amount of the gaseous product adsorption liquid are appropriately selected depending on the amount of gaseous products generated. For example, if 5111 points of the test liquid are applied, approximately 5μ of aqueous hydrochloric acid solution with a concentration of IN is applied. do.

In the third embodiment, the liquid capable of stopping the generation of gaseous products may be any liquid capable of stopping the generation of gaseous products, for example, in the case of ammonia gas, it is an acidic solution such as hydrochloric acid, sulfuric acid, phosphoric acid, etc., or water. The concentration and amount of this liquid are appropriately selected depending on the amount of gaseous products generated. For example, BUN
In the multilayer analytical tape for measurement, BNU concentration is 100 mg.
If one spot of 5Il of /di test liquid is applied, approximately 7Ii of aqueous hydrochloric acid solution of concentration IN is applied.

In the fourth embodiment, the flow of gas for removing gaseous products is small (at least, if the air around the spotting part can be replaced, the wind speed, air volume, air blowing time, and air blowing direction may be changed as appropriate). The air can be blown before the next test liquid is applied (or it can be blown continuously. Air is usually used as the gas to be flowed.

In the fifth aspect, ventilation around the spotting section can be improved by keeping the shutter open for a long time or by providing a ventilation door or the like and opening it.

In the sixth aspect, promptly sending out the site where the test liquid has been applied after photometry is completed without regard to preparations for applying the next different test liquid. Immediately after that, the part of the multilayer analysis tape where the test liquid was spotted is sent out from inside the incubator to the outside.

The first to sixth embodiments of the second invention are also similar to the first invention. .

In the present invention, gaseous bioacids that affect photometry and test components that generate gaseous products include ammonia gas and urea nitrogen, ammonia gas and ammonia, ammonia gas and taleatinin, and the like.

The integrated multilayer analytical element used in the present invention can be any conventionally proposed or usable multilayer analytical element cut into a long tape shape.
For example, JP-A-55-164356, JP-A-59
This is a multilayer analytical element described in JP-A-102388, JP-A-60-222769, JP-A-62-27006, and the like.

[Effect]

In the first embodiment of the first and second inventions, the multilayer analytical tape is conveyed longer than usual, so that the gaseous products generated from the photometered spotting part reach the next spotting part. The time is increased and the density is reduced to the extent that it does not affect the next photometry.

In the second embodiment, by spotting the gaseous product adsorbing liquid, the gaseous product adsorbing liquid adsorbs and removes the high concentration gaseous product generated from the photometered spotting part.

In the third embodiment, the reaction stop solution is applied to prevent the generation of gaseous products and to adsorb and remove the gaseous products that have already been generated.

In a fourth embodiment, the gas flow blows away the gaseous products around the spot.

In a fifth embodiment, the area around the spotting part is made permeable to improve the air flow around the spotting part, and the gaseous products are quickly diffused.

In the sixth embodiment, by immediately sending out the part where the test liquid has been spotted after photometry from inside the ink beta to the outside, gaseous products generated from the spotting part where photometry has been completed are removed from the spotting part. The concentration of the gaseous product is diluted to the extent that it does not affect the next photometry by preventing the gaseous product from stagnation in the vicinity of the sample and prolonging the time until the next different test liquid is applied. There is.

〔Example〕

An embodiment of the analysis method of the present invention will be described based on FIGS. 1 to 7.

First, an analysis apparatus for carrying out the analysis method of the present invention and a normal analysis method in which high concentration gaseous products are not generated will be explained based on FIGS. 1 and 2.

FIG. 1A is a perspective view of the analyzer, FIG. 1B is a plan view of the main parts of the analyzer 1 whose perspective view is shown in FIG. 1A, and FIG. 1C is a cross-sectional view taken along line X-X in FIG. FIG.

The illustrated chemical analysis device 1 is equipped with a transparent lid 2, and by opening this M2, a test liquid, a multilayer analysis tape 3, etc., which will be described below, are stored in and taken out from the device 1. There is. This device 1 is equipped with a test liquid storage means 4 that stores test liquids such as serum and urine in a circular arrangement. It is taken out by the attaching means 5 and spotted on the multilayer analysis tape 3. The multilayer analysis tape 3 is a plurality of types of analysis tapes each containing a reagent that selectively exhibits a color reaction with each specific chemical component or formed component that is the test component to be measured in the test solution. be. The unused portion of this multilayer analysis tape 3 is wound in the tape supply cassette 18, and the portion used for the above measurement is wound in the tape winding cassette 19.
It is wrapped inside and taken in.

The multilayer analytical tape 3 is wound into a cassette 18, 19 and placed in the device 1. The tape supply cassette 18 and tape winding cassette 19 are separated as shown in the figure. Multiple test items (test components) can be tested simultaneously using this device.
The analysis tape storage means 6 is constructed so that a plurality of tape supply cassettes 18 can be stored in parallel so that measurements can be performed.

The spotting means 5 has a spotting nozzle 7 at its tip, and is moved in a direction parallel to the rail 8 by a moving means 9 placed on the rail 8 to take out the test liquid from the test liquid storage means 4. , onto the multilayer analytical tape 3 pulled out from within the analytical tape storage means 6. Further, the spotting means 5 is configured to be movable in the vertical direction, and when the spotting means 5 is moved along the rail 8 by the moving means 9, the spotting means 5 is in an elevated position, and the spotting means 5 is in an elevated position. It is lowered during liquid removal, spotting, and cleaning, which will be described later.

The analysis tape on which the test liquid has been spotted is incubated (warmed) in an incubator as described later, and color development, discoloration, etc. are measured by a photometric means.

Control of the overall operation of the device 1, processing of measurement data, etc. are performed by a controller 11 and a computer 12 connected to the controller 11.

The computer 12 includes the device 1 and the computer 12.
A keyboard 14 for giving instructions to the device 1, a CRT display 15 for displaying auxiliary information for instructions, measurement results, etc., a printer 16 for printing out the measurement results, and a computer program for giving various instructions to the device 1 and commands to be input. A floppy disk device 17 is provided for storing and storing data such as measurements and measurement results.

The analytical tape storage means 6 is configured such that the spotting positions 22 of all the analytical tapes pulled out from therein are arranged in a straight line, and the spotting nozzle cleaning section 1 is arranged on this straight line.
0, and sample liquid extraction positions 4b in the storage means 4 having storage portions 4a arranged on the circumference are arranged.

The spotting means 5 moves in the direction in which the rail extends by means of a moving means 9 mounted on the rail 8, takes out the test liquid from the take-out position 4b, and spots it on the multilayer analysis tape 3 at the spotting position 22. .

The multilayer analysis tape 3 is stored in a tape supply cassette and loaded into the apparatus, and as it is used in the apparatus, it is sequentially wound into a tape winding cassette 19. The tape supply cassette 18 has an almost uniform temperature inside (for example, 1
The tape winding cassette 19 is stored in a winding chamber 51, and the tape winding cassette 19 is stored in a winding chamber 51.

An incubator 55 is arranged in the exposed part of the analysis tape 3 between the tape supply cassette 18 and the tape winding force cell 1-19, and is capable of holding the film therein and allowing it to pass therethrough one after another. A photometer 57 is disposed within the incubator 55 for reflective photometry of optical density due to a color reaction occurring on the analysis tape due to a specific component in the test liquid.

The multilayer analysis tape 3 is moved to the cold storage 50 by the rotation of the motor 53.
The incubator 5
The upper lid 55a of No. 5 rises in the direction of arrow B. When the multilayer analysis tape 3 has finished moving, the upper lid 55a descends in the direction of arrow C and holds the analysis tape 3. Next, the shutter 54 moves rightward in the figure to open the nozzle insertion hole 55b, and the nozzle 7 descends as shown in the figure through the nozzle insertion hole 55b, and the liquid is spotted from the nozzle 7 onto the analysis tape 3. . After that, the shutter 54 moves to the left to open the nozzle insertion hole 55.
b, and the inside of the incubator is maintained at a predetermined temperature (for example, 37° C.). The area on the multilayer analysis tape 3 where the test liquid is spotted and spread (the area indicated by diagonal lines in FIG. 3) is kept at a constant temperature in the incubator 55 for a predetermined period of time (for example, 4 minutes).

After or during the incubation, the optical density of the spotted portion of the multilayer analysis tape 3 is measured by the photometer 57. This density measurement is performed by irradiating the tape 3 with light containing a preselected wavelength emitted from the light irradiation means 57a and detecting the reflected light from the tape 3 with the photodetector 57b.

In this manner, when the spotting, incubation, and measurement of one test liquid are completed and a new portion of the analysis tape is pulled out from the tape supply cassette, the next test liquid can be placed. The analysis tape 3 remains in the incubator even after the measurement of one sample liquid is completed, and just before the spotting for the next analysis is performed, the tape part to be used for the next analysis moves to the spotting position. and will be transferred.

Next, a description will be given of a normal analysis process when the sample liquid generates a small amount of gaseous products using the above-mentioned analyzer.

First, as shown in FIG. 2(a), with the multilayer analysis tape 3 stopped, the shutter 54 is opened to expose the spotting part Ao of the multilayer analysis tape 3 (opening step).

Next, as shown in Fig. 2, etc.), the spotting means (nozzle 7)
is horizontally moved to a position above the spotting hole (nozzle insertion hole 55b) and then lowered to approach above the spotting portion Ao. Thereafter, a predetermined amount of the test liquid, for example 5I, is applied (spotting step).

Then, as shown in FIG. 2(C), after removing the nozzle 7 from the nozzle insertion hole 55b, the shutter 54 is returned to close the nozzle insertion hole 55b and cover the spotting area Ao for incubation. process).

After heating the spotting part Ao inside the incubator 55 in this state, as shown in FIG.
7b (photometric process).

Furthermore, as shown in FIG. 2(e), the multilayer analysis tape 3
Feed one frame and insert the next spotting part A1 into the nozzle insertion hole 5.
Position it below 5b (1g! feeding process).

Thereafter, the same procedure is repeated to apply spots A2 and A in turn.

... Spot the test liquid on An and measure the light.

An embodiment of the analysis method according to the first aspect of the first invention will be described based on FIGS. 3(a) to 3(d).

FIGS. 3(a) to 3(d) are plan views showing how the test liquid is applied to the multilayer analysis tape. In these figures, the right side is the multilayer analysis tape supply (feeding) cassette 18 side,
The left side is the winding cassette 19 side, and the multilayer analysis tape 3 is conveyed from the right side to the left side. Further, arrow A indicates the position where the test liquid is spotted by the spotting means (nozzle) 7.

First, as shown in FIG. 3(a), the latest test liquid α0 is spotted on the spotting part Ao, and the spotting part A-
Test liquids α-1 to α-n are spotted on lA-n, and photometry has been completed.

If this spotted test liquid α0 has a low concentration of urea nitrogen as a result of photometry, the multilayer analysis tape 3 is conveyed one frame as shown in FIG. Position A1 at spotting position A. Then, as in the conventional method, the test liquid α1 is subjected to an incubation step and then photometrically measured in a photometry step. As a result of this photometry, if the test liquid α contains a high concentration of urea nitrogen, as shown in FIG. The multilayer analysis tape 3 is conveyed so that , are located at the spotting position. and,
As in the past, photometry is performed in the photometry process after the opening process, spotting process, and incubation process.
The high-concentration ammonia gas generated from the sample is diffused to a low concentration by the time it reaches the spot A, so the sample liquid α2
Accurate photometry is achieved with almost no effect on

If this test liquid α2 does not contain a high concentration of urea nitrogen, as shown in FIG. 3 (6), it is transported one frame as usual and the same process is repeated.

An embodiment of the chemical analysis method according to the second aspect of the first invention will be described based on FIGS. 4(a) to 4(d).

FIGS. 4(a) to 4(d) are plan views showing how the test liquid is applied to the multilayer analysis tape. In these figures, the multilayer analytical tape 3 is conveyed from the right side to the left side. Further, arrow A indicates the position where the test liquid A is spotted by the spotting means 7.

First, as shown in FIG. 4(a), the latest test liquid α0 is spotted on the spotting part Ao, and the spotting part 11 on the left side of the spotting part 11.
- Test liquids α-8...α-n have been applied to A-n, and photometry has been completed.

If this spotted test liquid α0 does not contain a high concentration of urea nitrogen as a result of photometry, the multilayer analysis tape 3 is conveyed one frame as shown in FIG. Place part A at spotting position A. Then, as in the conventional case, the test liquid α is spotted in the spotting step after the opening step, and then the light is measured in the photometry step after passing through the incubation step.

As a result of this photometry, if the test liquid α1 contains a high concentration of urea nitrogen, as shown in FIG. Place part ^8 at dot position A. Then, instead of spotting the test liquid α2 in the spotting process after the opening process, a hydrochloric acid solution (IN, 5 m) β0 as an ammonia adsorption liquid is spotted on the spotting portion A2.

Thereafter, the incubation step and the photometry step are omitted, and the process moves to the transport step, where the multilayer analysis tape 3 is transported one frame again, and the spotting section ^ is positioned at the spotting position. Then, through the opening process, the test liquid α2 is spotted in the spotting process, and further,
After the incubation process, photometry is performed in the photometry process.

At this time, the highly concentrated ammonia gas generated from the test liquid α is adsorbed by the hydrochloric acid solution β0 and diffused to the spotting area, so the area around the test liquid α2 is almost normal air. covered with Therefore, photometry of the test liquid α2 is performed accurately.

An embodiment of the chemical analysis method according to the third aspect of the first invention will be described based on FIGS. 5(a) to 5(d).

FIGS. 5(a) to 5(d) are plan views showing how the test liquid is applied to the multilayer analysis tape. In these figures, the multilayer analytical tape 3 is conveyed from the right side to the left side. Further, arrow A indicates the position where the test liquid A is spotted by the spotting means 7.

First, as shown in FIG. 5(a), the latest test liquid α0 is spotted in the spotting part Ao, and the spotting part A-I on the left side
. . . Test liquids α-1 . . . α-n are applied to A-n, and photometry has been completed.

If this spotted test liquid α0 does not contain a high concentration of urea nitrogen as a result of photometry, the multilayer analysis tape 3 is conveyed one frame as shown in FIG. Place the dot on point A at point A. Then, as in the conventional case, the test liquid α1 is spotted in the spotting process through the opening process, and further, the light is measured in the photometry process after passing through the incubation process.

As a result of this photometry, if the test liquid α1 contains a high concentration of urea nitrogen, the transportation step is omitted, and the hydrochloric acid solution (IN,
Spot 5jl of γ0 again on spotting section A+.

After this, as in the conventional case, the multilayer analysis tape 3 is conveyed one frame, the spotting part A2 is positioned at the spotting position A, and as shown in FIG. Test solution α2
is applied, followed by an incubation process and a photometry process.

At this time, in the spotting part A1, the test liquid α is
1 and the reagent in the multilayer analysis tape 3 has stopped, and no ammonia gas is generated, and the ammonia gas generated during the incubation process is adsorbed by the hydrochloric acid solution TO, so there is no ammonia around the spot A2. Almost no gas remains, so photometry of the test liquid α2 is performed accurately.

An embodiment of the chemical analysis method according to the fourth aspect of the first invention will be described based on FIG. 6.

FIG. 6 is an enlarged sectional view of the spotting position portion of the analyzer.

To perform a chemical analysis using the analyzer as described above, when the urea nitrogen concentration of the test liquid is low, the opening step, spotting step, incubation step, photometry step, and transport step are repeated as in the conventional method.

Next, when the urea nitrogen concentration of the test liquid is high, after measuring the urea nitrogen concentration of the test liquid α1 spotted on the spotting portion A1 in the photometry step, the multilayer analysis tape 3 is fed one frame in the transport step. Thereafter, without proceeding to the push-open process, a determining means for determining whether or not the urea nitrogen concentration is sequentially connected to the photometric means 57b, a control means for operating the blower means based on a signal from the determining means, and a control means for operating the blowing means based on a signal from the determining means. A gas flow (flow of air) is blown from the blast pipe 84 by a blowing means (none of which is shown) that sends air to the blast pipe in response to a signal from the means. Due to this gas flow, the ammonia gas ordered from the test liquid α is
It is removed from around the next spotting portion At. After this, the process moves to the opening process and spotting process similar to the conventional process.

An example of the analysis method according to the fifth aspect of the first invention will be described.

With this analysis method, if the urea nitrogen concentration in the test solution is low,
The opening process, spotting process, incubation process, photometry process, and conveyance process are repeated in the same manner as before.

Next, when the urea nitrogen concentration of the test liquid is high, the opening step, the spotting step, the incubation step, the photometric step, and the conveying step are repeated as in the conventional method, but the urea nitrogen concentration sequentially connected to the photometric means 57b is high. A determining means for determining whether or not the upper lid 55a
A driving means (none of which is shown) for opening and closing the lid 55a is used to keep the top lid 55a open for about 10 seconds or longer than usual. This improves the air permeability around the spotting part At, the ammonia gas generated from the test liquid α1 flows out to the outside, and the ammonia gas concentration around the spotting part A2 becomes extremely small.

An example of the analysis method according to the sixth aspect of the first invention will be described.

With this analysis method, if the urea nitrogen concentration in the test solution is low,
Repeat the opening process, . . . transport process as in the past.

Next, when the urea nitrogen concentration of the test liquid is high, the opening step, the spotting step, the incubation step, and the photometry step are performed as in the conventional method. In response to a signal from the photometer, the photometry means sends out a signal to finish photometry, regardless of the preparation operation for the next spotting of a different test liquid, and this signal immediately causes one or two frames, that is, the next one. The multilayer analysis tape 3 is conveyed so that either the spotting section or the next spotting section is located at the spotting position. As a result, the time when the spotting part of the test liquid containing high concentration of urea nitrogen is away from the next spotting part or the next spotting part becomes longer, and the ammonia gas generated from the spotting part is transferred from the spotting part to the outside. The ammonia gas concentration around the spotting site becomes extremely small.

Each aspect of the second invention is also similar to that of the first invention described above.

In the above explanation, in the incubator part, the upper 1155
In this example, a spotting aperture (nozzle insertion hole) 55b is provided in a, and a shutter 54 is provided on the upper surface of the spotting aperture so as to be movable in the horizontal direction in the figure. Ta. The configuration shown in FIG. 1D can also be used as the incubator part of the measuring device. The ink beta part shown in FIG. 1D is provided with a cover 59 that covers the top 1i55a of the ink beta, a spotting opening (nozzle insertion hole) 55b is provided in the cover 59, and a spotting opening (nozzle insertion hole) 55b is provided below the spotting opening 55b of the cover. A shank 54 that can be slid laterally is provided on the side surface. When placing a spot on the multilayer analysis tape 3, the shutter 54 is moved to the left to open the opening 55b, and the upper ll5 is synchronously moved.
5a first moves upward so as not to contact the top layer (unfolded M) of the multilayer analytical tape 3, and then moves to the left to expose the spotting site of the multilayer analytical tape 3. Next, the nozzle is inserted through the opening 55b, and the test liquid is spotted on the spotting site of the multilayer analysis tape.

After the spotting is completed, the upper lid 55a covers the part of the multilayer analysis tape where the test liquid has been spotted, and the shutter 54 closes the opening 55b of the cover 59 in the reverse operation to the above.

A device in which the cover 59 is provided with a shutter 54 is preferred because the location of the shutter is far from the multilayer analytical tape.

Next, experimental results comparing a practical example according to the present invention and a comparative example will be explained.

Using the sample (1) containing high density urea nitrogen and the normal sample (2), the measured diameter of sample (2) of sample I was measured.

Comparative example: In a normal analysis method where the test liquid is normal, the transport distance of one frame (distance between the centers of the spotting parts, the same applies hereinafter) is 18 m.
m, and the shutter was open for 2 seconds.

Example 1 In the analysis method according to the first aspect of the first invention, the transport distance of one frame is 18 mm, and the transport distance of two frames is 36 mm.
mm.

Example 2 In the analysis method according to the second aspect of the first invention, 5j11 spots of a hydrochloric acid solution with a concentration of IN were deposited as an ammonia adsorption liquid. Further, the conveyance processing for one frame was set to 18M.

Example 3 In the analysis method according to the third aspect of the first invention, 74 points of hydrochloric acid solution with a concentration of IN were deposited as a reaction stop solution.

Example 4 In the analysis method according to the fourth aspect of the first invention, a gas flow was blown for 5 seconds at an air flow rate of 1 m/sec.

Example 5 In the analysis method according to the fifth aspect of the first invention, the shutter 6 was kept open for 10 seconds.

Table 1 shows the measurement results for sample ①.

Note that the multilayer analysis tape 71, incubation process, photometry process, etc. were all the same.

The measurement results for Sample H are shown in Table 1.

From the results shown in Table 1 and above, it can be seen that the analytical method of the present invention has significantly improved measurement accuracy.

〔Effect of the invention〕

By configuring the present invention as described above, it is possible to minimize the influence of gaseous products generated from the previous sample liquid, so that accurate measurements can always be made.

[Brief explanation of drawings]

Fig. 1A is a perspective view of an analyzer that carries out the analysis method according to the present invention, Fig. 1B is a plan view of the main parts of the same, Fig. 1C is a sectional view taken along the line x-X in Fig. 1B, and Fig. 1D is according to the present invention. FIG. 3 is a cross-sectional view of an incubator portion of another configuration of an analysis apparatus for carrying out an analysis method. FIG. 2 is a diagram showing a normal analysis process when the sample liquid is normal, and FIGS. 3(a) to (d) are samples of an embodiment of the analysis method according to the first aspect of the first invention. 4(a) to 4(d) are plan views showing the state of spotting of the test liquid in an embodiment of the analysis method according to the second aspect of the first invention. Figures 5(a) to 5(d) are plan views showing the test liquid spotting state in an embodiment of the analysis method according to the third aspect of the first invention, and FIG. 7 is an enlarged partial view of the analytical device used for carrying out the analytical method according to the fourth aspect of the invention.
FIG. 8 is an engraving of a schematic cross-sectional drawing of a conventional analytical device, FIG. 1A, FIG.・Outer frame 77
...Shutter 55a...Top lid 55.7B...
・Incubator 59...Cover 57b, 79
...Optical measurement probe 76... Spotting hole 7... Nozzle 84... Blower pipe 85... Spotting means 87
・・・Opening/closing door

Claims (2)

[Claims] (1) A transport step in which a long integrated multilayer analysis element is intermittently transported by predetermined lengths, and a predetermined spotting site of the developed layer of the multilayer analysis element transported in the transport step. a spotting step in which the test liquid is spotted on the spot, an incubation step in which the spotting site where the test liquid is spotted in the spotting step is incubated; Repeating the photometry step of measuring the optical density value of the color of the multilayer analysis element based on a direct or indirect color reaction between the test component contained in the test liquid and the reagent composition contained in the multilayer analysis element. The analytical method includes a step of determining the amount of a gaseous product that affects the color reaction during the photometry step, and the reaction between the components in the test liquid and the reagent composition is included. includes a reaction that produces a gaseous product that affects the coloring reaction, and the test component in an amount that produces a large amount of the gaseous product is contained in the spotted test liquid. When it is determined in the determination step that the gaseous product An analysis method characterized by adding a step of processing a multilayer analysis element so as to substantially eliminate the influence caused by (2) A transport step in which a long integrated multilayer analysis element is intermittently transported by predetermined lengths, and a predetermined spotting site of the developed layer of the multilayer analysis element transported in the transport step. a spotting step in which the test liquid is spotted on the spot, an incubation step in which the spotting site where the test liquid is spotted in the spotting step is incubated; Repeating the photometry step of measuring the optical density value of the color of the multilayer analysis element based on a direct or indirect color reaction between the test component contained in the test liquid and the reagent composition contained in the multilayer analysis element. In the analytical method, when the multilayer analytical element contains a reagent composition that generates a gaseous product that affects the color reaction by reaction with the test component, the following different test liquids are used: treating the multilayer analytical element in such a way that the effects due to said gaseous products are substantially eliminated in the color reaction at the next predetermined spotting site of the multilayer analytical element where is spotted and photometered; Analysis method characterized by the addition of a process