JP3317315B2 - Dry analysis film storage device - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a dry analysis method having a reagent layer which causes a change in optical density due to a chemical reaction, a biochemical reaction or an immune reaction with a predetermined biochemical substance contained in a sample solution such as blood or urine. The present invention relates to a storage device for storing a film, such as a film supplier of a biochemical analyzer.

[0002]

2. Description of the Related Art In recent years, the content of specific biochemical components or chemical components contained in a sample solution or the activity value thereof, or the content of a formed component is only obtained by spotting and supplying small droplets of the sample solution. 2. Description of the Related Art A dry integrated multilayer analysis film (also referred to as a multilayer analysis element or a multilayer analysis element) capable of quantitatively analyzing an amount has been developed and put into practical use. Also, a filter paper type test piece and a single-layer or multi-layer test piece obtained by improving the same have been proposed, and some of them have been put to practical use.

In order to quantitatively analyze a biochemical component or a chemical component in a sample solution using such a dry analysis film, the sample solution is applied to a development layer when the dry analysis film has a development layer. In the case where the developing layer is not provided, the reagent layer is directly spotted on the reagent layer, and this is kept at a constant temperature (incubation) in an incubator (incubator) for a predetermined time to cause a color reaction (a dye formation reaction or a discoloration reaction of the indicator dye). Then, the chemical analysis slide is irradiated with irradiation light for measurement including a wavelength selected in advance by a combination of a predetermined biochemical substance or chemical substance in the sample solution and a reagent contained in the dry analysis film, and the optical density is measured. Measured, and from this optical density, the sample solution is measured using a calibration curve representing the correspondence between the optical density determined in advance and the substance concentration of a predetermined biochemical substance or chemical substance. And requests a predetermined material concentration biochemicals or chemicals (content) or activity value.

The integrated multilayer dry analysis film has a structure in which at least one reagent layer containing a reagent is provided on a support made of an organic polymer, and more preferably, a developing layer is provided above the reagent layer. And formed on a dry analysis film piece having a predetermined shape such as a square or a rectangle. For the purpose of automatic operation, the dry analysis film piece has been put into practical use as a chemical analysis slide in which an organic polymer mount is used to hold the dry analysis film piece. In addition, the present inventors have developed a technique of directly loading a dry analytical film piece without the above-mentioned mount into a cartridge, storing the cartridge in a storage device (film supplier) of a biochemical analyzer, and sequentially taking out the measurement. is suggesting.

[0005] This storage device includes a storage container capable of reducing the humidity of the sealed interior, and a holder stored in the storage container. The holder has a plurality of holding portions for holding a cartridge. Is formed. The holder is configured to be able to move the cartridge held in each holding portion to an outlet of the dry analysis film formed in the storage container, so that the dry analysis film can be sequentially taken out of the cartridge moved to the outlet. Has become.

[0006]

When the storage device is maintained or inspected, when it is out of order, or when the dry analysis film is not used for a certain period of time, all the cartridges stored in the storage container are replaced with another low humidity. It is necessary to evacuate to a cold place (for example, a refrigerator). An entrance for replacing the cartridge is formed in the storage container,
Only one cartridge can be taken out from this entrance. For this reason, conventionally, even when all the cartridges in the storage container are retracted, it is necessary to take out the cartridges one by one from the entrance, and the taking out work is troublesome.

[0007] It is desirable that the cartridges once retracted are returned to the same holding section that was held before each cartridge was taken out. For this purpose, it is necessary to determine which cartridge was held in which holding section. The cartridge must be restored while checking. Such a restoration work is troublesome and error-prone.

[0008] Such a problem is not limited to the storage device configured so that the dry analysis film is held on the holder via the cartridge, but a large number of dry analysis films are directly held on the holder of the holder. This also occurs in the case of such a configuration.

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and an object of the present invention is to make it possible to easily perform a retreating operation and a restoring operation of a dry analysis film held in a holding body and housed in a housing container. An object of the present invention is to provide a device for storing a dry analysis film.

[0010]

According to a first aspect of the present invention, there is provided an apparatus for accommodating a dry analysis film according to the present invention, wherein a holder for holding the dry analysis film in a plurality of holders can be taken in and out of an accommodation container. It is characterized by having.

The dry analysis film may be held directly in the holding section, or may be loaded in a cartridge and held in the holding section via the cartridge.

The holder can be configured to be movable in the container so that the dry analysis film held in the plurality of holders can be sequentially conveyed to an outlet formed in the container. In this case, a driving mechanism that is configured to be freely detachable from the holder when the holder is taken in and out, and that moves the holder is provided.

[0013] A moisture absorbent for absorbing moisture in the container can be accommodated in the container together with the holder so as to be able to be taken in and out.

The term "hygroscopic body" refers to a dehumidifier, a humidifier, a humidifier, or the like.

In the apparatus for storing a dry analytical film according to the present invention, the evacuation time calculating means for calculating the evacuation time from when the holder is taken out of the container to when it is restored in the container, and the evacuation time is calculated. Means for comparing the evacuation time calculated by the means with a preset reference time and determining whether or not the dry analysis film held on the holder is in a usable state based on the comparison. And output means for outputting the result of the determination by the comparison and determination means.

The evacuation time calculating means calculates an effective evacuation time from the evacuation time on the basis of a correction value set in advance with respect to the evacuation environment of the holding body (during evacuation, under what environment, etc.). Can be configured.
In this case, the comparison determination means is configured to compare the effective evacuation time with the reference time and make a determination based on the comparison.

In addition, the evacuation time calculating means sets the storage time in which the dry analysis film held by the holding body was stored in the storage container before the holding body was taken out of the storage container into the effective evacuation time. It can be configured to calculate a more effective evacuation time in consideration of the evacuation time. In this case, the comparison determination means is configured to compare the more effective evacuation time with the reference time and make a determination based on the comparison.

[0018]

According to the apparatus for storing a dry analysis film of the present invention, the holder holding the dry analysis film can be taken in and out of the storage container. For this reason, when the dry analysis film is to be evacuated during maintenance and inspection of the storage device, when it breaks down, or when the dry analysis film is not used for a certain period of time, remove the dry analysis film together with the holder from the storage container, and It can be evacuated to an evacuation place such as a refrigerator. Therefore, the evacuation operation can be performed very easily.

Also, when restoring the evacuated dry analysis film in the storage container, the holder may be returned to the storage container together with the holder. Since the dry analysis film is held in the same holder as before being removed from the container, it is necessary to check which dry analysis film was held in which holder of the holder as in the case of the conventional restoration work. Absent. Therefore, the restoration work can be performed very easily.

According to the apparatus for storing a dry analysis film of the present invention, the holder is configured to be movable within the storage container, and is provided with a drive mechanism configured to be detachable from the holder when the holder is taken out. When the body is taken in and out, it is possible to easily take in and out the holding body without taking any trouble to engage and disengage the driving mechanism and the holding body.

According to the apparatus for storing a dry analysis film of the present invention in which a moisture absorbent that can be taken in and out together with the holder is provided in the storage container, when the dry analysis film is retracted and restored,
It is possible to easily transfer the moisture absorbent together with the holder. For this reason, the holder and the moisture absorber can be transferred in the same container until the holder is removed from the storage container and transferred to the refrigerator, or until the holder is returned to the storage container from the refrigerator. This makes it possible to prevent deterioration of the dry analysis film during transfer.

According to the apparatus for storing a dry analysis film of the present invention provided with the evacuation time calculation means, the comparison determination means and the output means, the dry analysis film which has been changed to a state in which it cannot be used during evacuation is erroneously detected. Can be prevented from being used.

Normally, the rate at which the dry analysis film deteriorates depends on the storage environment of the dry analysis film. Therefore, the effective evacuation time calculated based on a correction value set in advance for the evacuation environment of the holder is considered. According to the dry analysis film accommodating apparatus of the present invention which makes the determination based on the determination, it can be more accurately determined whether or not the dry analysis film is in a usable state.

Usually, the dry analysis film is deteriorated while the dry analysis film is placed in the container. If the time in the storage container is shorter than the evacuation time, the deterioration of the dry analysis film in the storage container may be ignored.

According to the dry analysis film accommodating apparatus of the present invention, the judgment is made based on the more effective retreat time calculated in consideration of the accommodation time of the dry analysis film until the holder is taken out of the accommodation container. In addition, it is possible to more accurately determine whether or not the dry analysis film is in use.

[0026]

Embodiments of the present invention will be described below with reference to the accompanying drawings. First, FIG. 1 is a perspective view showing a schematic configuration of a biochemical analyzer incorporating a storage device according to one embodiment of the present invention.

The biochemical analyzer 10 includes a film storage device 11 (film supplier) that stores unused, substantially square or rectangular dry analysis film pieces 1, and a side of the film storage device 11. An incubator 12 for holding the dry analysis film piece 1 at a constant temperature for a predetermined time; a film transport means 13 for transporting the dry analysis film piece 1 from the film storage device 11 to the incubator 12; and a plurality of sample liquids such as serum and urine. Sample storage means 14 for storing
(Sampler), spotting means 15 for spotting the dry analysis film piece 1 until the sample liquid in the sample liquid storage means 14 is conveyed to the incubator 12 by the film conveying means 13, and disposed below the incubator 12. Measuring means 16 provided.

As shown in FIG. 2, the dry analytical film piece 1 is made of, for example, PET (polyethylene terephthalate).
A reagent layer 1b including a spreading layer is laminated on a light-transmitting support 1a (base film) made of a plastic film such as a plastic film. Further, an abrasion-resistant protective layer (not shown) made of a fibrous material such as cloth is laminated on the reagent layer 1b as necessary, or the protective layer is configured to also serve as a spreading layer. May be.

In the dry state before use, the dry analytical film piece 1 tends to be curved with the reagent layer 1b inside, and the degree of curvature differs depending on the type of the reagent layer 1b and the degree of drying. When the reagent layer 1b of the dry analysis film piece 1 is mixed with a predetermined component of the sample liquid dropped from the nozzle tip 88 of the spotting means 15, a color reaction (dye formation reaction) is performed after a constant temperature holding (incubation) for a predetermined time. ) Is contained (a chemical analysis reagent or an immunoanalysis reagent is included), and a reagent layer 1b set for each chemical component or material component to be measured in the sample solution corresponding to the measurement item Are prepared in plural types.

The dry analytical film piece 1 is accommodated in a cartridge 20 as shown in FIG. 3 for each measurement item. The cartridge 20 accommodates a large number of dry analytical film pieces 1 in a stacked state with the light-transmitting support 1a facing down, and is loaded into the film accommodating device 11 as shown in FIG.

The details of the film storage device 11 are shown in FIG.
A description will be given based on FIG. 4 and FIG. FIG. 4 is a sectional view showing the internal structure of the film storage device 11, and FIG. 5 is a bottom view.

As shown in FIG. 4, the film storage device 11
The container includes a storage container 24 and a nest 22 as a holding member stored in the storage container 24. The storage container 24 includes a concave storage container main body 24a and a lid 24b that opens and closes an upper portion of the storage container main body 24a. The lid 24b is rotatably mounted via a hinge 26, and the housing body 24a and the lid 24b are provided with a lock mechanism (not shown) which is disengaged when the lid 24b is opened and closed.

The nest 22 is formed in an annular shape, and is rotatably and detachably held by the container main body 24a. As shown in FIG. 1, the nest 22 is formed with a plurality of holding portions 22a into which the cartridges 20 are loaded. Each of the holding portions 22a extends from a cartridge insertion opening 33 with an opening / closing door 32 formed on a lid 24b. The loaded cartridge 20 is loaded and held.

A cylindrical dehumidifier storage part 25 is formed at the center of the storage container main body 24a.
Inside, a dehumidifying agent 27 is loaded. The dehumidifying agent 27 keeps the inside of the container 24 in a dry state at a low temperature. In this embodiment, the replacement of the dehumidifying agent 27 is performed by opening and closing the lid 24b. However, an opening for replacing the dehumidifying agent 27 may be provided in the lid 24b.

Further, an outlet 23 for taking out the dry analysis film piece 1 from each cartridge 20 is formed on the lower surface of the container main body 24a, and a shutter 28 for opening and closing the outlet 23 is provided. As shown in FIG. 5, the shutter 28 is urged in a direction to close the outlet 23 by a spring 29, and when the dry analysis film piece 1 is taken out,
It is configured to be pressed by a cam 31 rotated and driven by a shutter motor 30 to open the outlet 23.

A tooth portion 22b is formed below the outer peripheral surface of the nest 22 so as to mesh with a rotating gear 21a of a nest motor 21 as a driving mechanism. The nest 22 is driven to rotate by the nest motor 21. Predetermined cartridge 20
Is controlled to be conveyed to the position of the outlet 23.

At the time of maintenance and inspection of the film accommodating device 11 or at the time of breakdown, or when the dry analysis film piece 1 accommodated in the accommodating container 14 is not used for a certain period of time, the lid 24b is opened and the accommodating container 24a is opened. The nest 22 is taken out while holding the cartridge 20. If the nest 22 is pulled up directly, the tooth portion 22b of the nest 22 and the rotating gear 21 of the nest motor 21
The engagement with a is easily released. The removed nest 22 is a box body 61 and a cover body as shown in FIG.
It is stored in an evacuation box 60 composed of 62. As shown in FIG. 4, a spacer portion is provided on the lower surface of the nest 22 to secure a distance between the mounting surface and the lower surface of the cartridge 20 so that the cartridge 20 does not contact the mounting surface when the nest 22 is mounted. The cartridge 20 does not contact the bottom surface of the evacuation box 60 even if the nest 22 is placed in the evacuation box 60.

The evacuation box 60 also contains a dehumidifying agent 27 together with the nest 22, so that the evacuation box 60 is kept in a low humidity state. The nest 22 is transferred and stored in a low-humidity, low-temperature refrigerator while being stored in the evacuation box 60. When returning the nest 22 to the accommodation container 24 again, the nest 22 may be taken out of the evacuation box 60 taken out of the refrigerator and restored in the accommodation container 24. Nest 22
When the nest motor 21 is
a again engages with the teeth 22b of the nest 22. In the present embodiment, the cartridge held in the restored nest 22
20 (strictly speaking, the dry analysis film piece 1 loaded in the cartridge 20) is determined to be ready for use,
A determination system is provided for notifying the operator of the biochemical analyzer 10 of this. FIG. 7 shows an outline of this determination system.

As shown in FIG. 7, the determination system comprises a control means 100 comprising a computer, a switch SW1 connected to the control means 100, a keyboard 50, an output means 51 comprising a CRT and a speaker. As shown in FIG. 4, the switch SW1 is attached to the inner bottom surface of the container main body 24a, and is pressed by the nest 22 when the nest 22 is inside the container 24, and is pressed when the nest 22 is taken out. It is configured to be released and turned off. Whether the switch SW1 is on or off is detected by the signal processing means 102 in the control means 100, and the signal processing means 102 outputs the time when the switch SW1 is switched from on to off (the nest 22 is taken out). The time when the nest 22 is restored from the clock 101 and the time when the nest 22 is restored (the time when the nest 22 is restored) are read from the clock 101 and are stored in the storage means 103.

The storage means 103 comprises a RAM or the like, and is configured to store various data input via the keyboard 50 in addition to writing the time. For example, each holding portion 22a of the nest 22 has
20 is held, and the cartridge is
Data such as the time at which the cartridge 20 is loaded or a reference time (effective time during which the cartridge 20 can be used) preset for each type of the cartridge 20 are stored.
Each holding unit 22a is numbered, and the above data is stored in correspondence with the number of each holding unit 22a,
Each time 20 is replaced, it is rewritten by the signal processing means 102.

In addition, the control means 100 compares the evacuation time with the reference time with the evacuation time calculating means 104 for calculating the evacuation time from when the nest 22 is taken out to when it is restored.
Comparison determination means is provided for determining whether or not the dry analysis film piece 1 of each cartridge 20 held in the nest 22 is in a usable state based on the comparison.

Hereinafter, an example of the determination processing by this determination system will be described with reference to the flowchart shown in FIG. In this determination processing, first, the number of the holding section 22a in which the cartridge 20 is loaded and the type of the loaded cartridge 20 are input to the signal processing means 102 via the keyboard 50, and the signal processing means 102 stores these data in the storage means. At the same time, the time when each cartridge 20 is loaded is read from the clock 101 and the time is stored in the storage means 103 (step S1). The storage means 103 has a
A correction value is stored for calculating the reference time T ₀ and the effective retreat time T ₂ for each type of the cartridge 20.

Next, the signal processing means 102 sets the switch S
It is determined whether W1 has been turned off (step S2),
The time t ₂ to time i.e. nested 22 turned off when it is determined that the off is issued from the container 24 clock 10
1 and store it in the storage means 103 (step S3). Next, in step S4, the signal processing means
102 determines whether the switch SW1 is turned on, if it is determined that turned on, reads a time t ₃ when the time ie nests turned on is restored to the container 24 from the clock 101, stores it It is stored in the means 103 (step S5).

In step S6, the evacuation time calculating means 10
4 is the saving time T from the saving time t ₂ and the restoring time t ₃ read from the storage means 103 by the signal processing means 102.
₁ (= t ₃ −t ₂ ) is calculated. Further saving time calculating means 104, the effective saving period T ₂ from the saving period T ₁ based on the correction value stored in advance in the storage unit 103 for each type of the cartridge 20 with respect to saving environment cartridge
It is calculated every 20 (step S7). This correction value is obtained, for example, when the nest 22 is retracted in the refrigerator or when the nest 22 is retracted in the same environment as the storage container 24. The deterioration speed of the piece 1 may be grasped in advance, and may be determined according to the difference in the deterioration speed. In the present embodiment, based on the deterioration speed of the dry analysis film piece 1 when the nest 22 is stored in the storage container 24, the time elapsed in the retreat place is determined by the time that the nest 22 is stored in the storage container 24. The correction value is set so that, for example, how much elapsed time corresponds to it.

Next, in step S8, the evacuation time calculating means 104 determines the loading time t ₁ of the cartridge 20 and the nest 22
The storage time T ₃ (= t ₂ −t ₁ ) in the storage container 24 is calculated in the cartridge 20 from the evacuation time t _{2 of} step S 2,
9, the storage time T ₃ and the effective evacuation time T
_{From 3} , the more effective evacuation time T ₄ (= T ₂ + T ₃ )
Is calculated for each cartridge 20.

In the next step S10, the comparison judgment means 10
5 compares the reference time (effective time) T ₀ previously stored for each type of the cartridge 20 in the storage means 103 with the more effective evacuation time T ₄ calculated as described above, and In S11, it is determined whether or not there is a cartridge 20 satisfying T ₀ <T ₄ . If T ₀ <T ₄ , that is, if there is a cartridge 20 that is not in a usable state, in step S12, the comparison determination unit 105 outputs the number of the holding unit 22a holding the cartridge 20 to the output unit.
In addition to displaying on the CRT as 51, an alarm sound is generated from the speaker to call the operator's attention. When all the cartridges 20 are in a usable state, the dry analysis film pieces 1 of each cartridge 20 are used as they are for normal biochemical analysis. Further, when the remaining time of the usable state is within a predetermined time, it is possible to display the alarm on a CRT and to generate an alarm sound from a speaker.

Hereinafter, a biochemical analyzer will be described with reference to FIG.
10 Other configurations will be described.

In the incubator 12 shown in FIG. 1, a disk-shaped main body 40 is rotatably supported by a rotation drive mechanism 41 at the lower center, and the dry analysis film pieces 1 are accommodated on the circumference of the main body 40 at predetermined intervals. Cells 42 are arranged,
The dry analysis film piece 1 is incubated in the cell 42.

The main body 40 of the incubator 12 has a lower disk 45 made of a metal having a flat lower upper surface, and an upper disk (not shown) made of metal disposed on the lower disk 45. Is formed by bulging upward in an annular shape,
A gap is formed between the lower end of the outer periphery and the upper surface of the lower disk 45 to form a side opening of the cell 42. Main body 40
A heater is built in the inside, and the dry analysis film piece 1 is heated and maintained at a predetermined temperature (for example, 37 ° C.).

Although not shown, the lower disk
A photometric window is opened at a predetermined interval in 45 corresponding to the position where the cell 42 is formed, and a film press for fixing the inserted dry analytical film piece 1 at a predetermined position is disposed in the cell 42. Below the main body 40 at the position, a photometric head 95 of the measuring means 16 is provided.

The transport means 13 for transporting the dry analytical film piece 1 from the film storage device 11 to the incubator 12 comprises:
The suction cup 70 for taking out the dry analysis film piece 1 from the cartridge 20 and the dry analysis film piece 1 held by the suction cup 70 are held from below with the development layer 4 facing upward. And a horseshoe-shaped transfer member 73 inserted into the cell 42 of the incubator 12 from the side opening, and the dry analysis film piece 1 held by the transfer member 73 in the cell 42 of the incubator 12 is transferred to the cell 42. And a suction cup 76 for holding and holding from below.

The suction cup 70 for removal is provided so as to be able to move back and forth and up and down by a moving mechanism (not shown), draws the dry analysis film piece 1 out of the cartridge 20, and conveys it to the spotting position. Further, the holding suction cup 76 is provided so as to be able to move up and down by a driving mechanism (not shown), and moves into and out of the cell 42 from a photometric window opened on the bottom surface of the cell 42 of the incubator 12.

On the other hand, a film discharge means 17 is provided at a film discharge position of the incubator 12, and a suction cup 81 for sucking and lifting the dry analytical film piece 1 in the cell 42 after measurement, and a suction cup 81 for discharge. The horseshoe-shaped transfer member 82 for receiving the dry analysis film piece 1 lifted at 81 and taking it out of the incubator 12 and the dry analysis film piece 1 taken out by the transfer member 82 are received and discarded. And a disposal suction cup 83 that is disposed in a disposal box 84.

The sample liquid storage means 14 includes a rotary drive mechanism 86
The rotary table 85 is rotated by the rotary table 85. A plurality of sample containers 87 containing a sample liquid are held on the outer periphery of the rotary table 85, and the sample containers 87 are sequentially moved to the supply position. Further, a nozzle tip 88 attached to the tip of a spotting nozzle 91 described later is accommodated on the inner peripheral side.

The spotting means 15 for spotting each sample solution in the sample container 87 onto the dry analysis film piece 1 conveyed to the incubator 12 has a spotting nozzle 91 for sucking and discharging the sample solution. The nozzle tip 88 in the form of a pipette is detachably attached to the tip of the spotting nozzle 91, and has a driving mechanism.
The sample liquid is moved up and down and rotatable by 92, sucks and moves the sample liquid from the sample liquid storage means 14, and is spotted on the dry analysis film piece 1 held on the transfer member 73. Further, the nozzle tip 88 of the tip of the spotting nozzle 91 of the spotting means 15 is replaced with a change of the sample liquid.

The spotted dry analytical film piece 1 is incubated in the incubator 12 and measured by the measuring means 16 disposed below the incubator 12. The measuring means 16 includes a dry analysis film piece 1.
A photometric head 95 for measuring an optical density by a color reaction between the sample and an analyte in a sample solution. Above photometric head
Reference numeral 95 denotes a light source for irradiating the reagent layer 1b with irradiation light for measurement including light of a predetermined wavelength, which is transmitted through the light-transmitting support 1a, and detects reflected light with a light detection element. Light from 96 (lamp) enters through an interference filter 97, and the light is irradiated on the reagent layer 1b in the photometric head 95. The filter 97 includes a plurality of discs 98 corresponding to the inspection items.
The disk 98 is rotated by a motor 99 to select a filter 97 having predetermined characteristics corresponding to the measurement item.

Incidentally, as the spectral means provided in the optical system for irradiating the light measuring head 95 with the measuring light, a diffraction grating may be used instead of using the interference filter 97 as described above.

The reflected light from the reagent layer 1b is optical information (specifically, the amount of light) corresponding to the amount of dye generated in the reagent layer 1b.
The reflected light carrying the optical information is incident on the photodetector of the photometric head 95, is subjected to photoelectric conversion, and is transmitted to the determination unit via the amplifier. The determination unit determines the optical density of the dye generated in the reagent layer 1b based on the level of the input electric signal, and determines the concentration (content) or the activity value of a predetermined biochemical substance in the sample solution. It is calculated based on the principle of the colorimetric method.

The measurement by the biochemical analyzer 10 will be described. First, the dry analytical film stored at a predetermined humidity from the cartridge 20 corresponding to the measurement item from the film storage device 11 by the suction cup 70 for removal of the transport means 13. Remove piece 1. The dry analysis film piece 1 held by the suction cup 70 is transferred to the transfer member 73 with the reagent layer 1b facing upward, and the sample liquid is spotted on the reagent layer 1b.

This spotting is performed by the spotting nozzle 91 of the spotting means 15.
After attaching the nozzle tip 88 to the tip of the
The nozzle tip 88 is moved onto the predetermined sample container 87 of FIG.
Is immersed in the sample liquid, and a predetermined amount of the sample liquid is sucked into the nozzle tip 88. Then, the spotting nozzle 91 is moved to the center of the dry analysis film piece 1 on the transfer member 73, and then the spotting nozzle 91 is moved downward to make the nozzle tip.
From 88, a predetermined amount of the sample solution is dropped onto the reagent layer 1b of the dry analysis film piece 1. The dropped sample liquid is spread and diffused,
Mix with reagents.

The dry analytical film piece 1 after spotting is used as a transfer member.
73 transported to the incubator cell 42 to open the side opening
Inserted from 42a. When the dry analysis film piece 1 is heated to a predetermined temperature by a predetermined incubation in a sealed state in the incubator cell 42, the reagent layer 1b causes a color reaction (dye formation reaction). Then, at every predetermined time or after a predetermined time elapses during the color reaction, the optical density of the dye generated by the color reaction is measured by the photometric head 95 of the measuring means 16.

The dry analysis film that can be stored in the dry analysis film storage device of the present invention includes a dry analysis film piece without a mount as described in the above embodiment and a slide type dry analysis film with a mount. Films, filter paper type dry analysis films (test pieces) and the like are applicable.

[Brief description of the drawings]

FIG. 1 is a perspective view schematically showing a biochemical analyzer provided with a storage device according to one embodiment of the present invention.

FIG. 2 is a perspective view showing a spot state of a dry analysis film piece.

FIG. 3 is a perspective view showing a state in which a dry analysis film piece is removed.

FIG. 4 is a sectional view showing the internal structure of the storage device shown in FIG. 1;

FIG. 5 is a bottom view of the storage device shown in FIG. 4;

FIG. 6 is a perspective view schematically showing an evacuation box.

FIG. 7 is a block diagram of a determination system connected to the storage device.

FIG. 8 is a flowchart illustrating an example of a determination process;

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Dry analysis film piece 11 Film storage device 20 Cartridge 21 Nest motor 22 Nest (holding body) 22a Holding section 23 Outlet 24 Storage container 24a Storage container main body 24b Lid 26 Hinge 27 Desiccant 33 Insertion port 50 Keyboard 51 Output means 60 Evacuation box 100 Control means 101 Clock 102 Signal processing means 103 Storage means 104 Evacuation time calculation means 105 Comparison judgment means

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-4-357460 (JP, A) JP-A-63-215948 (JP, A) JP-A-58-156833 (JP, A) 30962 (JP, A) JP-A-2-216463 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) G01N 35/04 G01N 1/28

Claims (6)

(57) [Claims] 1. A dry analysis film incorporated in a biochemical analyzer, wherein the dry analysis film is stored in a storage container while being held by a plurality of holding portions of a holder, and can be sequentially removed from the storage container. The storage device for a dry analysis film, wherein the holding body holding the dry analysis film in the plurality of holding portions is configured to be able to be taken in and out of the storage container. 2. The holding body is configured to be movable in the storage container so that the dry analysis film held by the plurality of holding units can be sequentially transported to an outlet formed in the storage container. 2. The apparatus according to claim 1, further comprising a drive mechanism configured to be detachable from the holder when the body is taken in and out, and provided with a drive mechanism for moving the holder. 3. The dry analysis film according to claim 1, wherein a moisture absorbent for absorbing moisture in the container is accommodated in the container so as to be able to be taken in and out together with the holder. Accommodation equipment 4. An evacuation time calculating means for calculating an evacuation time from when the holding body is taken out of the storage container to when it is restored in the storage container, and an evacuation time calculated by the evacuation time calculation means. A comparison determination unit that compares the dry analysis film held by the holder with the reference time set in advance to determine whether or not the dry analysis film held in the holder is in a usable state; 4. An apparatus for accommodating a dry analysis film according to claim 1, further comprising an output unit for outputting a determination result of the unit. 5. The evacuation time calculation means is configured to calculate an effective evacuation time from the evacuation time based on a correction value set in advance with respect to the evacuation environment of the holding body. The apparatus according to claim 4, wherein the effective evacuation time is compared with the reference time, and the determination is made based on the comparison. 6. The evacuation time calculating means determines the storage time, in which the dry analysis film held in the holder is stored in the storage container, until the holder is removed from the storage container, in the effective time. The more effective evacuation time is calculated in consideration of the effective evacuation time, and the comparing / determining means compares the more effective evacuation time with the reference time, and based on the comparison, 6. The apparatus for storing a dry analysis film according to claim 5, wherein the apparatus is configured to make a determination.