JPH02219574A - Method for supporting microorganism - Google Patents
Method for supporting microorganismInfo
- Publication number
- JPH02219574A JPH02219574A JP1040090A JP4009089A JPH02219574A JP H02219574 A JPH02219574 A JP H02219574A JP 1040090 A JP1040090 A JP 1040090A JP 4009089 A JP4009089 A JP 4009089A JP H02219574 A JPH02219574 A JP H02219574A
- Authority
- JP
- Japan
- Prior art keywords
- carrier
- microorganisms
- water
- absorbing
- suspension
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 68
- 238000000034 method Methods 0.000 title abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 42
- 238000010521 absorption reaction Methods 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 229920006395 saturated elastomer Polymers 0.000 claims abstract description 5
- 238000011068 loading method Methods 0.000 claims description 2
- 239000000725 suspension Substances 0.000 abstract description 26
- 230000000813 microbial effect Effects 0.000 abstract description 12
- 241000894006 Bacteria Species 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 3
- 239000010802 sludge Substances 0.000 abstract description 3
- 230000001877 deodorizing effect Effects 0.000 abstract description 2
- 238000005121 nitriding Methods 0.000 abstract 1
- 239000003415 peat Substances 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 description 10
- 235000016068 Berberis vulgaris Nutrition 0.000 description 4
- 241000335053 Beta vulgaris Species 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000010800 human waste Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Treating Waste Gases (AREA)
- Biological Treatment Of Waste Water (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は微生物担持方法に係り、特に、吸水性担体に所
定量の微生物を効率良く、均一に付着固定させる微生物
担持方法に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a method for supporting microorganisms, and more particularly to a method for supporting microorganisms in which a predetermined amount of microorganisms is efficiently and uniformly adhered and immobilized on a water-absorbing carrier.
［従来の技術］
し尿、下水、その他の廃水の臭気ガスの処理装置等とし
て、ビート等の吸水性を有す虫担体を湿潤させ、これに
微生物を担持させてなる処理層を有する生物反応装置が
用いられている。[Prior Art] A biological reaction device having a treatment layer made by moistening a water-absorbing insect carrier such as beet and carrying microorganisms as a treatment device for odor gas from human waste, sewage, and other wastewater. is used.
従来、このような生物反応装置においては、保水した担
体を反応装置に充填した後、この充填層に微生物（菌体
）懸濁液（活性汚泥を含む）を散布ないし環流させるこ
とにより、微生物な担体に付着させていた。Conventionally, in such a biological reaction device, after filling the reaction device with a water-retaining carrier, microorganisms (bacterial cells) suspension (including activated sludge) is sprayed or circulated in the packed bed to remove microorganisms. It was attached to a carrier.
［発明が解決しようとする課題］
上記従来の微生物担持方法では、担体表面への微生物の
付着が十分でないために、次のような問題があった。[Problems to be Solved by the Invention] The conventional method for supporting microorganisms described above has the following problems because the adhesion of microorganisms to the surface of the carrier is insufficient.
■ 微生物の付着効率が悪いため、必要以上に多量の微
生物を散布ないし環流することが要求される。このため
、設備が大型化し、作業が煩雑となり、処理コストが高
騰する。■ Because the adhesion efficiency of microorganisms is poor, it is necessary to spray or circulate a larger amount of microorganisms than necessary. For this reason, the equipment becomes larger, the work becomes complicated, and the processing cost increases.
■ 担体の微生物担持量を把握することができない。■ It is not possible to determine the amount of microorganisms carried on the carrier.
■ 担体への微生物の付着が均一でないため、担持され
た微生物の装置内分布が不均一なものとなる。■ Since the attachment of microorganisms to the carrier is not uniform, the distribution of the supported microorganisms within the device becomes uneven.
■ 担体の表面積を有効に利用できず、効果的に装置と
しての有効処理表面積が十分に得られない。(2) The surface area of the carrier cannot be used effectively, and a sufficient effective processing surface area cannot be effectively obtained as an apparatus.
このため、従来の微生物担持方法による生物反応装置で
は、効率的な生物反応処理を行なうことができなかった
。For this reason, it has not been possible to perform efficient biological reaction treatment in biological reaction devices using conventional microbial loading methods.
本発明は上記従来の問題点を解決し、微生物を均一かつ
効率的に担体に付着固定することができる微生物担持方
法を提供することを目的とする。An object of the present invention is to solve the above conventional problems and provide a method for supporting microorganisms that can uniformly and efficiently adhere and fix microorganisms to a carrier.
［Ｈ題を解決するための手段］
本発明の微生物担持方法は、吸水量が飽和吸水量の３０
％以下である吸水性担体に、微生物を含む液を接触させ
て該液を該担体に吸収させることにより微生物を該担体
に担持させることを特徴とする。[Means for Solving Problem H] The method for supporting microorganisms of the present invention has a water absorption amount of 30% of the saturated water absorption amount.
The method is characterized in that the microorganisms are supported on the carrier by bringing a liquid containing the microorganisms into contact with a water-absorbing carrier having a water-absorbing capacity of % or less and absorbing the liquid into the carrier.
即ち、本発明者は、担体への微生物担持機構について鋭
意検討を重ねた結果、低含水率の吸水性担体の吸水作用
を利用することにより、微生物をより均一かつ効率的に
担持させることがで鎗ることを見出し、本発明を完成さ
せた。That is, as a result of intensive studies on the mechanism of supporting microorganisms on carriers, the present inventors have found that microorganisms can be supported more uniformly and efficiently by utilizing the water-absorbing action of a water-absorbing carrier with a low water content. He discovered this and completed the present invention.
以下に本発明の詳細な説明する。The present invention will be explained in detail below.
本発明の方法においては、飽和吸水量の３０％以下の吸
水量となりつる低含水率の吸水性担体（以下、「低含水
率担体」という、）を用いる。In the method of the present invention, a water-absorbing carrier with a low water content (hereinafter referred to as "low water content carrier") that absorbs water at 30% or less of the saturated water absorption amount is used.
従って、必要に応じて乾燥させて、その含水率を調整す
る。この場合、含水率は上記範囲内で特に制限はなく、
後工程の吸水工程において、必要とする吸水量に応じて
適宜含水率を選定する。Therefore, it is dried as necessary to adjust its moisture content. In this case, the moisture content is not particularly limited within the above range,
In the subsequent water absorption step, the water content is appropriately selected depending on the required amount of water absorption.
しかして、用いる吸水性担体の生物反応時の含水率と、
上記低含水率担体の含水率とから、低含水率担体を生物
反応時の含水率にするために必要な添加水量を求める。Therefore, the water content of the water-absorbing carrier used during the biological reaction,
From the water content of the low water content carrier, the amount of water required to bring the low water content carrier to the water content required for biological reaction is determined.
即ち、低含水！Ｉ！担体の含水率ａ％、生物反応時の含
水率ｂ％、担体の乾燥重量Ｗとすると、添加水量は
で求められる。In other words, low water content! I! Assuming that the water content of the carrier is a%, the water content at the time of biological reaction is b%, and the dry weight of the carrier is W, the amount of added water is determined by:
次に、このようにして算出した添加水量に、担持させる
所定量の微生物を含む液、例えば微生物を懸濁させた微
生物懸濁液を調製する。そしてこの微生物懸濁液と前記
低含水率担体とを接触させ、担体全体に微生物懸濁液の
全量を十分に吸水させる。接触方法としては、特に制限
はないが、具体的には、次の■又は■の方法を採用する
ことができる。Next, a liquid containing a predetermined amount of microorganisms to be supported, for example, a microorganism suspension, is prepared by suspending microorganisms in the amount of added water calculated in this manner. Then, this microorganism suspension is brought into contact with the low water content carrier, and the entire amount of the microorganism suspension is sufficiently absorbed into the entire carrier. There are no particular restrictions on the contact method, but specifically, the following method (1) or (2) may be employed.
■ カラムに充填した低含水率担体に微生物懸濁液を流
下させる。■ Let the microbial suspension flow down onto a low moisture content carrier packed in a column.
■ 微生物懸濁液中に低含水率担体を浸漬する。■ Immerse the low moisture content carrier in the microbial suspension.
（その後、カラムに充填す、る。）
このようにして微生物懸濁液と低含水率担体とを接触さ
せることにより、微生物懸濁液はその全量が担体に吸収
される。このため、この微生物懸濁液を吸収した後の担
体は、生物反応に必要な含水率であり、かつ、予め設定
した所定量の微生物を担持するものとなる。(Then, the column is filled.) By bringing the microbial suspension into contact with the low water content carrier in this manner, the entire amount of the microbial suspension is absorbed by the carrier. Therefore, the carrier after absorbing this microorganism suspension has a moisture content necessary for biological reaction and supports a predetermined amount of microorganisms.
なお、本発明において用いる吸水性担体としては特に制
限はなく、ビート、おがくず等を用いることができる。The water-absorbing carrier used in the present invention is not particularly limited, and beets, sawdust, etc. can be used.
また、微生物懸濁液としても特に制限はなく、脱臭菌、
硝化菌等の菌体を懸濁させたものや活性汚泥の水分量を
調整したものを用いることができる。There are also no particular restrictions on the use of microorganism suspensions, including deodorizing bacteria,
A suspension of microbial cells such as nitrifying bacteria or activated sludge with an adjusted water content can be used.
［作用］
吸水性担体を乾燥して得られる低含水率担体は、高い吸
水性を有することから、予め所定濃度に調製された所定
量の微生物懸濁液と接触させることにより、この微生物
懸濁液のほぼ全量を均一に吸収する。このため、この微
生物懸濁液の水量と微生物量を予め設定することにより
、所定量の微生物量が担持された所定含水率の担体な容
易に得ることができる。しかして、用いた微生物のほぼ
全量を担持させることができるため、必要とする微生物
を最小限におさえることができる。[Function] Since the low water content carrier obtained by drying the water-absorbing carrier has high water absorption, this microbial suspension can be removed by contacting it with a predetermined amount of microbial suspension prepared in advance to a predetermined concentration. Absorbs almost the entire amount of liquid evenly. Therefore, by presetting the amount of water and the amount of microorganisms in this microorganism suspension, it is possible to easily obtain a carrier having a predetermined water content and carrying a predetermined amount of microorganisms. Since almost the entire amount of microorganisms used can be supported, the number of microorganisms required can be kept to a minimum.
即ち、微生物懸濁液と低含水率担体とを接触させた際に
、微生物懸濁液中の微生物は、担体内には入らず、あた
かも濾過されるような形で、担体表面上に付着する。担
体の吸水は、担体全体で行なわれるため、担体の表面全
体に微生物が付着することになる。That is, when a microorganism suspension is brought into contact with a low water content carrier, the microorganisms in the microorganism suspension do not enter the carrier, but adhere to the surface of the carrier as if being filtered. . Since the carrier absorbs water throughout the carrier, microorganisms will adhere to the entire surface of the carrier.
また、この際、微生物懸濁液中の微生物は、液の吸収と
ともに殆どすべて担体に付着する。従って、微生物懸濁
液中の微生物量が担体の付着菌量となる、これにより、
懸濁液の微生物量を調整することで、担体への付着量を
決定することが可能とされる。Moreover, at this time, almost all of the microorganisms in the microorganism suspension adhere to the carrier as the liquid is absorbed. Therefore, the amount of microorganisms in the microorganism suspension becomes the amount of bacteria attached to the carrier.
By adjusting the amount of microorganisms in the suspension, it is possible to determine the amount of microorganisms attached to the carrier.
［実施例］
以下に実施例及び比較例を挙げて本発明をより具体的に
説明する。[Example] The present invention will be described in more detail with reference to Examples and Comparative Examples below.
実施例１
吸水性担体としてビート（含水率５５％：飽和吸水量の
５２％）を用い、これを乾燥させて含水率を１０％とし
た。なお、このものの生物反応時の含水率は７０％であ
る。乾燥により得られた低含水率担体１４０ｇ（乾燥重
量として）に、微生物として硫化メチル分解菌をｓ ｍ
ｇ／ｇ−乾燥ビート付着させるために、微生物０．１４
０ｇを下記の計算により求めた水量３１１ｇに懸濁させ
た微生物懸濁液を調製した。Example 1 Beet (water content 55%: 52% of saturated water absorption) was used as a water-absorbing carrier and was dried to a water content of 10%. Note that the moisture content of this product during biological reaction is 70%. Methyl sulfide-degrading bacteria as microorganisms were added to 140 g (dry weight) of the low moisture content carrier obtained by drying.
g/g - for dry beet adhesion, microorganisms 0.14
A microorganism suspension was prepared by suspending 0g in a water amount of 311g determined by the following calculation.
得られた微生物懸濁液中に低含水率担体を浸漬したとこ
ろ、その全量が担体に吸収された。When a low water content carrier was immersed in the obtained microbial suspension, the entire amount was absorbed by the carrier.
得られた微生物担持担体を走査型電子顕微鏡により調べ
たところ、微生物が均一に付着していることが判明した
。When the obtained microorganism-carrying carrier was examined using a scanning electron microscope, it was found that the microorganisms were uniformly attached.
比較例１
吸水性担体の乾燥を行なわなかったこと以外は実施例１
と同様にして微生物懸濁液中に浸漬したところ、微生物
懸濁液はその約１／３しか吸収されなかった。Comparative Example 1 Example 1 except that the water-absorbing carrier was not dried.
When immersed in a microbial suspension in the same manner as above, only about 1/3 of the microbial suspension was absorbed.
［発明の効果］
以上詳述した通り、本発明の微生物担持方法によれば、
容易かつ効率的に微生物の担持を行なうことができ、次
のような優れた効果が達成される。[Effects of the invention] As detailed above, according to the method for supporting microorganisms of the present invention,
Microorganisms can be supported easily and efficiently, and the following excellent effects can be achieved.
■ 使用する微生物量を必要最小限におさえることがで
き、微生物コストが低減される。■ The amount of microorganisms used can be kept to the minimum necessary, reducing microorganism costs.
■ 設定量の微生物量を担体に付着させることができる
。このため、担体に担持されている微生物量を把握する
ことができる。■ A predetermined amount of microorganisms can be attached to the carrier. Therefore, the amount of microorganisms supported on the carrier can be ascertained.
■ 担体全体に微生物を付着させることができるため、
担持の有効表面積が増加し、結果的に装置の有効処理表
面積が高められ、装置の処理効率の向上、装置設備の小
型化が図れる。■ Because microorganisms can be attached to the entire carrier,
The effective surface area of the support is increased, and as a result, the effective processing surface area of the device is increased, and the processing efficiency of the device can be improved and the device equipment can be downsized.
■ 担体ないし装置への微生物分布が均一となり、装置
の処理効率が向上する。■ The distribution of microorganisms on the carrier or device becomes uniform, improving the processing efficiency of the device.
Claims (1)
体に、微生物を含む液を接触させて該液を該担体に吸収
させることにより微生物を該担体に担持させることを特
徴とする微生物担持方法。(1) Microorganisms are supported on the carrier by contacting a liquid containing microorganisms with a water-absorbing carrier whose water absorption amount is 30% or less of the saturated water absorption amount and causing the liquid to be absorbed by the carrier. Microorganism loading method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1040090A JPH02219574A (en) | 1989-02-20 | 1989-02-20 | Method for supporting microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1040090A JPH02219574A (en) | 1989-02-20 | 1989-02-20 | Method for supporting microorganism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH02219574A true JPH02219574A (en) | 1990-09-03 |
Family
ID=12571190
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1040090A Pending JPH02219574A (en) | 1989-02-20 | 1989-02-20 | Method for supporting microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02219574A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006068732A (en) * | 2004-08-06 | 2006-03-16 | Hiroshima Univ | Water area environment improving material and water area environment improving method using the same |
-
1989
- 1989-02-20 JP JP1040090A patent/JPH02219574A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006068732A (en) * | 2004-08-06 | 2006-03-16 | Hiroshima Univ | Water area environment improving material and water area environment improving method using the same |
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