JPH02209900A - Water soluble hem iron powder, production thereof and iron supplying drink containing same powder - Google Patents

Abstract

PURPOSE:To obtain the title powder capable of dissolving in the range of wide pH range and processing into drink, candy, chocolate, jury cake, biscuit, bread, UDON (Japanese noodle), etc. CONSTITUTION:At least one kind of protein and surfactant is added to an aqueous alkali solution of hem iron and the blend solution is dried to provide the aimed hem iron powder capable of dissolving in neutral and acidic region.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to a water-soluble heme iron powder, a process for its production, and an iron-supplementing food or drink containing the powder. The water-soluble heme iron powder of the present invention is a novel substance useful for preventing and improving iron deficiency anemia.

Prior Art and its Problems Conventionally, it has been necessary to supplement iron by some method for the purpose of preventing and improving iron deficiency anemia, especially in growing men and women, and women of childbearing age and during pregnancy. Various non-heme iron salts, such as ferrous sulfate, are used as food additives. However, since non-heme iron is absorbed mainly in the duodenum and upper jejunum in the form of inorganic salts, it is easily affected by gastric acid and various dietary factors, and the absorption rate is as low as 5-10% (in general, the absorption rate is low at 5-10%). (promotes in acidic conditions and decreases in alkaline conditions).

On the other hand, since heme iron has a special absorption route, its absorption is not hindered by the factors that inhibit absorption of non-heme iron. That is, Adam's turn (
In human experiments conducted by Adam Turnbull and others, the absorption of hemoglobin (a complex of heme and globin) in healthy subjects was more than twice that of ferrous sulfate (
Journal of Clinical Investigation (J, CIi.

Invest, )-4010), 1897.1962
).

For the reasons mentioned above, heme iron is the best way to replenish iron, but it is not desirable to consume large amounts of meat, liver, etc. that contain large amounts of heme iron.

Therefore, it is preferable to easily ingest heme iron extracted and purified from the blood of food animals (particularly cows) in the form of suitable foods and drinks. For this reason, heme iron must be soluble not only in the alkaline region where it is originally soluble, but also in the acidic and neutral regions, and in a form that can be easily processed into foods and drinks using conventional methods. However, heme iron in such a form has not yet been obtained.

Means for Solving the Problems In view of the current situation as described above, the inventor provides heme iron in a form that is easy to process into foods and drinks that are soluble in neutral and acidic regions, and foods and drinks containing the same. For this purpose, we conducted extensive research. That is, the present inventor took advantage of the dilute alkali solubility of heme iron, first dissolved heme iron in a dilute alkali aqueous solution, then added various substances, and added an organic acid or mineral acid to the resulting mixture. It was observed whether heme iron precipitated under neutral or acidic conditions. As a result, it was found that by incorporating protein or a surfactant into a dilute alkaline aqueous solution of heme iron, heme iron does not precipitate (and dissolves in a stable state) even when the solution is neutral or acidic. Furthermore, it was discovered that heme iron powder obtained by drying this heme iron solution as it is or after making it neutral or acidic by freeze-drying etc. can be easily dissolved in the neutral and acidic region, and the present invention was completed. reached.

The present invention relates to a water-soluble heme iron powder comprising at least one of a protein and a surfactant and heme iron, which is characterized by being soluble in a neutral and acidic region, an iron-supplementing food and drink containing the same, and an alkaline heme iron powder. The present invention provides a method for producing water-soluble heme iron powder by including at least one of a protein and a surfactant in an aqueous solution and then drying the solution.

The heme iron in the present invention includes heme, heme proteins such as hematin, hemin, and hemoglobin, heme peptides obtained by decomposing heme proteins using acids or enzymes, or mixtures thereof. But it's okay.

In preparing the water-soluble heme iron powder of the present invention, first, heme iron is dissolved in a dilute alkaline aqueous solution, and then the protein and/or surfactant is directly dissolved in the aqueous solution, or the protein aqueous solution and/or surfactant The aqueous solution is mixed with a dilute alkaline aqueous solution of heme iron.

In this case, the dilute alkaline aqueous solution for dissolving heme iron has a pH of about 10 to about 13, preferably a pH of about 11 to about 1.
The sodium hydroxide aqueous solution, potassium hydroxide aqueous solution, etc. described in No. 2 are used.

The protein or surfactant may be used alone, in combination with two or more proteins or surfactants, or in combination with one or more proteins and one or more surfactants. I can do it.

Examples of proteins include serum albumin, ready-made albumin, serum β- and γ-globulin, globin, casein, gelatin, lactoglobulin, animal proteins such as lactoferrin, vegetable proteins such as soybean protein and wheat protein, elastase, kallikrein, Enzymes such as trypsin, amylase, lipase, peroxidase, catalase, and cytochrome C can be used.

In addition, as surfactants, sodium n-dodecyl sulfate, sodium cholate, sodium taurocholate,
Cetylpyridinium chloride, cetyltrimethylammonium bromide, dodecyltolumethylammonium bromide, dodecyltrimethylammonium chloride, lecithin, isooctynyl phenyl polyethoxy alcohol, polyoxyethylene sorbitan monolaurate, sorbitan laurate, etc. Negative, positive, amphoteric and nonionic surfactants can be used.

Preferably, the concentration of heme iron is from about 0.001 to about 1% w/v.

The concentration of protein or surfactant is about 0.0001 to about 10% w/v, preferably about 0.00%.
1 to about 0.1 w/v%.

The protein or surfactant-containing heme iron dilute alkaline aqueous solution obtained above is used as it is, or after it is made into a neutral or acidic solution, various known additives that are acceptable as food and drink additives are added thereto, if desired. A water-soluble heme iron powder can be produced by drying it by a method such as freeze drying or spray drying.

At this time, as the substance added to make the heme iron dilute alkaline aqueous solution neutral or acidic, for example, a mineral acid such as hydrochloric acid, an organic acid such as citric acid, etc. can be used. The concentration of addition varies depending on the type of protein, surfactant, and acid used.

Additives are not particularly limited; for example, carriers include wheat flour, rice flour, starch, cornstarch, soybeans, lactose, dextrin, etc.; sweeteners include sugar, glucose, fructose, stevia, aspartame, etc.; vitamins. Types include vitamin C1, vitamin A1, vitamin B1, vitamin B2, vitamin E, etc.; flavoring agents include vanillin, linalool, natural flavorings, etc.; acidulants include citric acid, malic acid, fumaric acid, succinic acid, tartaric acid, lactic acid, etc. ; Sodium chloride, potassium chloride, magnesium chloride, calcium chloride, etc. as electrolyte salts that enhance acidity; Others,
Mention may be made of natural product extracts such as coffee, black tea, green tea, guarana, apple juice, orange juice, gleb juice, etc. to enhance the flavor.

The above-mentioned additives can be added within the range of weights normally added to foods and drinks.

The water-soluble heme iron powder obtained as described above has a pH of 12 to 1.
It dissolves easily not only in alkaline regions but also in neutral and acidic regions. Therefore, due to this solubility, it can be easily processed into various food forms such as beverages, candies, drops, chocolates, jelly sweets, tablets, biscuits, kutsky, snacks, bread, buckwheat, radon, spaghetti, pasta, etc., as well as sauces. It can also be processed into seasonings such as , ketchup, and soy sauce.

Further, the heme iron powder of the present invention can be used as a powder, for example, as it is or dissolved in water or juice before use, or in the process of cooking various side dishes such as tsukudani, fried foods, and stir-fried foods. It can also be used in

EXAMPLES The present invention will be explained in more detail with reference to Test Examples and Examples, but the present invention is not limited thereto.

Test Example 1 0.04w/dissolved 0.0117w/v% hemin
After dissolving 0.05 w/v% of each of the substances shown in Table 1 below in a v% sodium hydroxide aqueous solution (pH 11), I
The pH was adjusted to 3 by adding N hydrochloric acid. The sample was left as it was, and the presence or absence of precipitates was observed. The results showed that the proteins bovine serum albumin, ready-made albumin, bovine serum β- and γ-globulins, gelatin, globin, lactoferrin, soy protein, wheat protein, elastase, kallikrein, trypsin, peroxidase and catalase as well as positive surfactants Certain solutions containing cetylpyridinium chloride and cetyltrimethylammonium bromide did not produce precipitates even after one week.

Glycine Phenylalanine Cystine Lysine Peptone Insulin Bradykinin Bovine serum albumin Ready-made albumin Bovine serum β-globulin Bovine serum γ-globulin Gelatin Globin Lactoferrin Soy protein Wheat protein Elastase Kallikrein Trypsin Peroxidase Catalase Glucose Sucrose +: Precipitate present -: Precipitate None Test Example 2 0.04w/v with 0.117w/v% hemin dissolved
% sodium hydroxide aqueous solution (pH 11), Test Example 1
Additives for which no precipitate was observed after 1 hour were added to 1.
After dissolving Ow/v%, IN citric acid was added to adjust the pH to 3.

Next, lactose 5. After adding Ow/v% and dissolving, freeze-dry,
Each powder was obtained. 1 w powder in tap water (pH 7)
/v% at room temperature, solubility and after 1 hour, 1
The presence or absence of precipitates was observed after 1 day and 1 week. The results showed that the proteins bovine serum albumin, ready-made albumin, bovine serum β- and γ-globulins, gelatin, globin, lactoferrin, soy protein, wheat protein, elastase, kallikrein, trypsin, peroxidase and catalase as well as positive surfactants A powder obtained by adding certain cetylpyridinium chloride and cetyltrimethylammonium bromide showed particularly good results, with no precipitate formed even after one week.

glycine phenylalanine cystine lysine peptone insulin bradykinin bovine serum albumin ready-made albumin bovine serum β-globulin bovine serum γ-globulin gelatin globin lactoferrin soy protein wheat protein elastase kallikrein trypsin peroxidase catalase glucose maltose starch cold sodium chloride sodium carbonate sulfate Potassium citrate-sodium oxalate Sodium n-dodecyl sulfate Sodium cholate Sodium taurocholate Lecithin Cetylpyridinium chloride Cetyltrimethylammonium bromide Isooctynyl phenylboethoxy alcohol (trade name "Trlton X-100" manufactured by Rohm & Haas) Poly Oxyethylene sorbitan monolaurate (trade name "Tween 20" manufactured by Atlas Powder Co., Ltd.) Sorbitan laurate +: Precipitates present - No precipitates Test Example 3 0.04 w/v in which 0.117 w/v% hemin was dissolved
% sodium hydroxide aqueous solution (pH 11), Test Example 1
Additives for which no precipitate was observed after 1 hour were added to 1.
After dissolving 0 w/v%, 5.0 w/v% of lactose was added and dissolved, followed by freeze-drying to obtain respective powders. 1 w/v% of the powder was added to a 0.2% aqueous citric acid solution (pH 3), and the solubility at room temperature and the presence or absence of precipitates after 1 hour, 1 day, and 1 week were observed. The results showed that the proteins bovine serum albumin, ready-made albumin, bovine serum β- and γ-globulin, gelatin, globin, lactoferrin, soy protein, wheat protein, elastase, kallikrein, trypsin, peroxidase and catalase, as well as positive surfactants. In the powder obtained by adding certain cetylpyridinium chloride and cetyltrimethylammonium bromide, no precipitates were formed even after one week, showing particularly good results.

Table 3: Isooctynyl phenylboethoxy alcohol (trade name: Tri ton+) 10 Bovine serum albumin Ready-to-use albumin Bovine serum β-globulin Bovine serum γ-globulin Gelatin Globin Lactoferrin Soy protein Wheat protein Elastase Kallikrein Trypsin Peroxidase Catalase Sodium n-dodecyl sulfate Sodium cholate Sodium taurocholate Lecithin Cetylpyridinium chloride Cetyltrimethylammonium monolaurate (trade name) Tween +: Precipitate present 1: Precipitate absent Example 1 0.04 w/v% sodium hydroxide aqueous solution (pH 11
) After dissolving 0.117 g of hemin in 0.9Ω with stirring, 1. Ow/v% bovine serum albumin aqueous solution 10
0- was added and further stirred. Add IN hydrochloric acid to pH 3,
After adjusting to 0, lactose of 5.0% Ow/v was added and freeze-dried to prepare the water-soluble heme iron powder of the present invention. Next, a drink (each component was dissolved in water to give 1j2) was prepared using the predetermined amounts of each component shown in the table below.

Ingredients Water-soluble hemin powder Sugar Sugar citrate Sodium citrate Sodium chloride Potassium chloride additionffi (g) 0.5 0, 8 0, 3 Flavoring total 1Ω This drink did not contain any insoluble substances, and even after one week of preparation. No precipitate was observed.

Example 2 0.04 w/v% sodium hydroxide aqueous solution (pH 11
) After dissolving 0.113 g of hematin in 0.9Ω with stirring, 1.0 w/v% egg albumin aqueous solution 100
- was added and further stirred.

After adding IN citric acid and adjusting the pH to 7.0,
/v% lactose was added and freeze-dried to prepare the water-soluble heme iron powder of the present invention. Next, predetermined amounts of each component shown in the table below were mixed to prepare a food in the form of a jelly confectionery.

Ingredients Added amount Water-soluble heme iron powder 400mg fructose
50g pectin 500mg
50% citric acid solution 0.4-20% sodium citrate solution 0.4aQ fragrance
Appropriate amount of water
20aQ This food showed no insoluble precipitates, and no change was observed even after one week of preparation.

Example 3 0.04 w/v% sodium hydroxide aqueous solution (pH 11
) 0.99 with stirring, 10 w/v% soybean protein (trade name: Fuji Pro CL, Fuji Essential Oil Co., Ltd.) aqueous solution 100- was added and further stirred. After adjusting the pH to 7 by adding IN hydrochloric acid, the mixture was freeze-dried to prepare the water-soluble heme iron powder of the present invention. Next, predetermined amounts of each component shown in the table below were mixed to prepare a powder mix for white bread.

component water soluble hemin powder strong flour sugar salt skim milk butter Addition amount (g) This bread powder mix is I didn't notice any changes.

One week after preparation

Claims (3)

[Claims] (1) A water-soluble heme iron powder that is composed of heme iron and at least one of a protein and a surfactant and can be dissolved in a neutral and acidic region. (2) An iron-supplementing food or drink containing the water-soluble heme iron powder according to claim 1. (3) A method for producing water-soluble heme iron powder by adding at least one of a protein and a surfactant to an alkaline aqueous solution of heme iron and then drying the solution.