JP2818827B2 - Iron supplement drink - Google Patents

Description

Description: FIELD OF THE INVENTION The present invention relates to iron supplemented beverages, and more particularly to novel beverages useful for preventing and ameliorating iron deficiency anemia.

Conventional technology and its problems Conventionally, it has been necessary to replenish iron in some way with the aim of preventing and ameliorating iron deficiency anemia, especially in developing men and women and in fertile and pregnant women. Various non-heme iron salts which are recognized as food additives such as ferrous sulfate are used. However, non-heme iron is mainly absorbed in the form of inorganic salts in the duodenum and upper jejunum, so it is susceptible to stomach acid and various dietary factors, and its absorption rate is as low as 5-10% (generally gastric acid). Accelerated in the acidity of the content and diminished in the alkaline).

On the other hand, since heme iron has a special absorption path, it is said that the absorption of the above-mentioned non-heme iron is not hindered by the above factors. That is, in experiments with humans such as Adam Turnbull, the absorption of hemoglobin (complex of heme and globin) in healthy subjects was more than twice that of ferrous sulfate (Journal).
Of Clinical Investigation (J. Cli.
Invest.), 41 (10), 1897, 1962).

Therefore, heme iron is the best for iron supplementation, but it is not preferable to ingest a large amount of meat, liver and the like containing a large amount of heme iron. Therefore, it is considered optimal to take heme iron in the form of a drink from the viewpoints of freshness, ease of drinking, ease of use, and nutrition. However, although heme iron is soluble in dilute alkali, it is insoluble in the acidic region, which is the general liquidity of drinks, so that heme iron is blended in a usual manner to produce a drink. , Heme iron precipitates as an insoluble matter. Therefore, it has been difficult to produce a drink that is dissolved in a stable state without precipitation of heme iron.

Means for Solving the Problems In view of the above-mentioned current situation, the inventors have made intensive studies for the purpose of providing a beverage in which heme iron is dissolved in a stable state. That is, the present inventor utilizes the dilute alkali solubility of heme iron, first dissolves heme iron in a dilute alkaline aqueous solution, then adds various substances, and adds an organic acid or a mineral acid to the obtained mixture. It was made acidic and it was observed whether or not heme iron was precipitated. As a result, it was found that by adding a protein or a positive surfactant to a dilute aqueous solution of heme iron containing a protein or a positive surfactant, the heme iron was dissolved in a stable state without precipitation even when the solution was acidified, and the present invention was completed. I came to.

The present invention relates to an iron supplement beverage in which heme iron is dissolved in a stable state without precipitation by adding a protein or a surfactant to a dilute aqueous solution of heme iron.

Heme iron in the present invention, heme, hematin, hemin, hemoglobin and other heme proteins and heme proteins are decomposed using an acid or an enzyme to reduce the molecular weight of the heme peptide, or any of these mixtures good.

In the preparation of the iron supplemented beverage of the present invention, first, heme iron is dissolved in a dilute alkaline aqueous solution, and then a protein or a surfactant is directly dissolved in the aqueous solution, or a solution prepared as a protein aqueous solution or a surfactant aqueous solution is mixed. Let it.

In this case, as a diluted alkaline aqueous solution for dissolving heme iron, an aqueous solution of sodium hydroxide or potassium hydroxide having a pH of 10 to 13, preferably 11 to 12 is used.

Proteins or surfactants may be used singly or as a mixture of two or more proteins or surfactants, or as a mixture of one or more proteins and one or more surfactants Can be done.

Proteins include serum albumin, egg albumin,
Serum β- and γ-globulins, globin, casein, gelatin, lactoglobulin, elastase, kallikrein, trypsin, amylase, lipase, and oxygen such as cytochrome C can be used.

As the surfactant, a positive surfactant such as cetylpyridinium chloride, cetyltrimethylammonium bromide, dodecyltrimethylammonium bromide or dodecyltrimethylammonium chloride can be used.

 The concentration of heme iron is preferably 0.001 to 1 w / v%.

The concentration of the protein or surfactant is 0.0001-10 w / v%,
Preferably it is 0.001-0.1 w / v%.

The protein or surfactant-containing heme iron diluted alkaline aqueous solution obtained above is blended with various known additives that are acceptable as beverage additives, and even as an acidic solution, without precipitation of heme iron, stable. Iron-supplemented beverages that are dissolved in a state can be produced. Additives are not particularly limited, for example, sugars, glucose, fructose, stevia, aspartame, etc. as sweeteners; vitamins, vitamin C, vitamin A, vitamin B1, vitamins
B2, vitamin E, etc .; vanillin, linalool, natural flavors, etc. as flavoring agents; citric acid, malic acid, fumaric acid, succinic acid, tartaric acid, lactic acid, etc., as acidulants; chloride as an electrolyte salt to enhance sourness Sodium, potassium chloride, magnesium chloride, calcium chloride and the like; and other natural product extracts such as coffee, black tea, green tea, guarana, apple juice, orange juice, and grape juice for enhancing the flavor.

The above additives can be added within the range of the compounding weight usually added to foods and drinks.

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to Test Examples and Examples, but the present invention is not limited thereto.

Test Example 1 The substances shown in Table 1 below were added to a 0.04 w / v% sodium hydroxide aqueous solution (pH 11) in which 0.0117 w / v% hemin was dissolved.
After dissolving at 0.05 w / v%, 1N hydrochloric acid was added to adjust to pH3. 1
After hours, one day and one week, the presence or absence of the precipitate was observed. As a result, proteins such as bovine serum albumin, egg albumin, bovine serum β- and γ-globulin, gelatin, globin, elastase, kallikrein, and trypsin, and positive surfactants cetylpyridinium chloride and cetyltrimethylammonium bromide were used. In the solution added, no precipitate was formed even after one week.

Test Example 2 Each substance shown in Table 2 below was added to a 0.04 w / v% sodium hydroxide aqueous solution (pH 11) in which 0.0117 w / v% hemin was dissolved.
After dissolving at 0.01 w / v%, 1N citric acid was added to adjust to pH3. After 1 hour, 1 day, and 1 week, the presence or absence of the precipitate was observed. As a result, proteins such as bovine serum albumin, egg albumin, bovine serum β- and γ-globulin,
In the solution containing gelatin, globin, elastase, kallikrein and trypsin, and the positive surfactants cetylpyridinium chloride and cetyltrimethylammonium bromide, no precipitate was formed after one week.

Test Example 3 Each of the substances shown in Table 3 below was dissolved in a 0.04 w / v% sodium hydroxide aqueous solution (pH 11) in which 0.0234 w / v% hechiman was dissolved, and 0.01 N citric acid was added thereto. The pH was adjusted to 3.5. After 1 hour, 1 day, and 1 week, the presence or absence of the precipitate was observed. As a result, proteins such as bovine serum albumin, egg albumin, bovine serum β- and γ-globulin, gelatin, globin, elastase, kallikrein, and trypsin, and positive surfactants cetylpyridinium chloride and cetyltrimethylammonium bromide were used. In the solution added, no precipitate was formed even after one week.

Example 1 A 0.04 w / v% aqueous solution of sodium hydroxide (pH 11) was adjusted to 0.9.
After dissolving 0.117 g of hemin with stirring, 100 ml of a 0.5 w / v% aqueous gelatin solution was added, and the mixture was further stirred. A predetermined amount of each component shown in the following table was dissolved in this mixed solution to prepare a beverage of the present invention.

No precipitate was observed even one week after the addition of the above components.

Example 2 A 0.04 w / v% aqueous solution of sodium hydroxide (pH 11) was adjusted to 0.9.
After dissolving 0.113 g of hematin with stirring, 0.1 w / v%
Was added and further stirred.
Dissolve a predetermined amount of each component shown in the following table in this mixture,
A beverage according to the invention was prepared.

No precipitate was observed even one week after the addition of the above components.

Example 3 0.08 w / v% aqueous solution of sodium hydroxide (pH 11) was adjusted to 0.9.
After dissolving 2.34 g of hemin while stirring, a 0.5 w / v% cetylpyridinium chloride aqueous solution 1 was added, and the mixture was further stirred. A predetermined amount of each component shown in the following table was dissolved in this mixture.

No precipitate was observed even one week after the addition of the above components.

──────────────────────────────────────────────────続 き Continued on the front page (58) Fields surveyed (Int. Cl. ⁶ , DB name) A23L 2/00 A23L 2/38 A61K 31/555 A61K 33/26 WPI (DIALOG)

Claims (1)

(57) [Claims] (1) serum albumin, oval albumin, serum β
-And γ-globulin, globin, casein, gelatin, lactoglobulin, elastase, kallikrein,
Trypsin, amylase, lipase and cytochrome C
At least one selected from the group consisting of cetylpyridinium chloride, cetyltrimethylammonium bromide, dodecyltrimethylammonium bromide, and a surfactant consisting of dodecyltrimethylammonium chloride
Iron supplement drink consisting of acidic heme iron aqueous solution containing seeds.