JPH0216430A - Apparatus for maintaining sample support at specified temperature - Google Patents
Apparatus for maintaining sample support at specified temperatureInfo
- Publication number
- JPH0216430A JPH0216430A JP1111924A JP11192489A JPH0216430A JP H0216430 A JPH0216430 A JP H0216430A JP 1111924 A JP1111924 A JP 1111924A JP 11192489 A JP11192489 A JP 11192489A JP H0216430 A JPH0216430 A JP H0216430A
- Authority
- JP
- Japan
- Prior art keywords
- conduit
- valve
- container
- sample support
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 claims abstract description 38
- 230000007246 mechanism Effects 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000007789 sealing Methods 0.000 claims abstract description 10
- 238000005192 partition Methods 0.000 claims abstract description 8
- 239000012530 fluid Substances 0.000 claims description 6
- 239000003566 sealing material Substances 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 238000010438 heat treatment Methods 0.000 description 6
- 238000004873 anchoring Methods 0.000 description 4
- 230000002906 microbiologic effect Effects 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 239000006263 elastomeric foam Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/02—Water baths; Sand baths; Air baths
Landscapes
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Fats And Perfumes (AREA)
- Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
- Supplying Of Containers To The Packaging Station (AREA)
- Investigating Strength Of Materials By Application Of Mechanical Stress (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Optical Measuring Cells (AREA)
- Signal Processing For Digital Recording And Reproducing (AREA)
Abstract
Description
【発明の詳細な説明】
本発明はミクロタイタープレート（微小滴定量プレート
）のような複数の凹所を有するプレートから成るサンプ
ル支持体を収容するようにした１つ又はそれ以上の容器
を備えているサンプル支持体を所定の温度に維持する装
置に関するものであり、該装置は制御された温度で液体
貯水槽中に熱を帯びている液体を維持する温度制御機構
と、貯水槽と容器との間に接続させた導管機構とを備え
ている。DETAILED DESCRIPTION OF THE INVENTION The present invention comprises one or more containers adapted to receive a sample support consisting of a plate with multiple wells, such as a microtiter plate. The apparatus relates to a device for maintaining a heated liquid in a liquid reservoir at a controlled temperature, and a temperature control mechanism for maintaining a heated liquid in a liquid reservoir at a controlled temperature, and a temperature control mechanism for maintaining a heated liquid in a liquid reservoir at a controlled temperature; and a conduit mechanism connected between the two.
かかる装置はオランダ特許明細書第１７３０６６号から
公知である。Such a device is known from Dutch Patent Specification No. 173066.
この公知の装置によれば、ミクロタイタープレートの形
式のサンプル支持体と協働するプレートが予め定めた温
度に加熱される。液体がサンプル支持体上に熱を転移さ
せるために液体によって加熱されたプレートからサンプ
ル支持体までの転移体が必要である。実際には、これは
サンプル支持体内のサンプルを加熱することに遅れを生
じさせる。この理由のために、サンプルが所望の温度を
達成する時点は余り正確には決定できない。これに関連
して、動力学的研究のような多くの用途を有する微小生
物学、酵素学および免疫学においては時間と温度に依存
する現象を研究することが非常に重要である。According to this known device, a plate cooperating with a sample support in the form of a microtiter plate is heated to a predetermined temperature. A transfer body from the plate heated by the liquid to the sample support is required for the liquid to transfer heat onto the sample support. In practice, this causes a delay in heating the sample within the sample support. For this reason, the point at which the sample achieves the desired temperature cannot be determined with much precision. In this context, it is of great importance to study time- and temperature-dependent phenomena in microbiology, enzymology and immunology, which have many applications such as kinetic studies.
本発明の目的はサンプルをかなり速い方法で所望温度に
加熱させることができ、その結果、所望温度が達成され
る時点がより正確に決定することができる装置を提供す
ることにある。The object of the invention is to provide a device which allows a sample to be heated to a desired temperature in a considerably faster manner, so that the point at which the desired temperature is achieved can be determined more precisely.
上記目的は本発明によれば、容器又はサンプル支持体と
に容器とサンプル支持体のプレートとの間に密封仕切り
室を形成するための密封機構を設け、液体貯水槽からの
仕切り室までの接続導管と仕切り室から液体貯水槽まで
の排出導管とを含んだ導管機構を備え、接続導管と排出
導管の少なくとも１つには閉鎖可能な弁機構を備えるこ
とによって達成される。According to the invention, the above object is achieved by providing a container or a sample support with a sealing mechanism for forming a sealed partition between the container and the plate of the sample support, and connecting a liquid reservoir to the partition. This is achieved by providing a conduit system including a conduit and a discharge conduit from the compartment to the liquid reservoir, and providing at least one of the connecting conduit and the discharge conduit with a closable valve mechanism.
これに関連して、サンプルのまわりに流れる温度制御媒
体を備えることは、例えばオランダ特許出願第７３０８
７８５号又はヨーロッパ特許出願第２３６９５７号明細
書からそれ自体公知である。しかし、そこでは温度制御
媒体は空気であり、空気は所望温度への招来はサーモス
タット付き液体によっての温度制御よりゆっくり生じ、
更に、同一温度変化に関してかなり大きな容量が搬送さ
れねばならない。In this connection, providing a temperature-controlled medium flowing around the sample can be used, for example, in Dutch patent application no. 7308
785 or from European Patent Application No. 236,957. However, there the temperature control medium is air, which brings about the desired temperature more slowly than temperature control by a thermostatic liquid;
Furthermore, for the same temperature change a considerably larger volume has to be transported.
更に、サンプル支持体を密封空間に配置させ。Furthermore, the sample support is placed in a sealed space.
加熱より空気循環を維持させる恒温器の加熱はヨーロッ
パ特許出願第２３８３１３号明細書から公知である。A heating incubator which maintains air circulation rather than heating is known from European Patent Application No. 238,313.
米国特許第３，５０５，０２３号明細書には、サンプル
を受容するために下方に延在する凹部を備えた上面と、
該上面の下側に設けた流体入口部と出口部とを備えてい
る温度調節流体室とを有するサンプル支持体が示されて
いる。これらの入口部と出口部とをホース又は同様の導
管に接続することはむしろ煩わしく、かなりの数のサン
プル支持体の場合には複雑な接続構造となる。更に、サ
ンプルを凹部に挿入することは入口部と出口部が凹部へ
の自由な接近を幾らか妨害する位置において行われねば
ならず、所望の温度に迅速に加熱する必要がある場合に
流体室を空にすることが望ましい。U.S. Pat. No. 3,505,023 discloses a top surface with a downwardly extending recess for receiving a sample;
A sample support is shown having a temperature controlled fluid chamber with a fluid inlet and outlet located below the top surface. Connecting these inlets and outlets to hoses or similar conduits is rather cumbersome and, in the case of a considerable number of sample supports, results in complex connection structures. Furthermore, the insertion of the sample into the recess must be carried out in a position where the inlet and outlet parts somewhat obstruct free access to the recess, and the fluid chamber must be closed if rapid heating to the desired temperature is required. It is preferable to leave it empty.
日本の特許アブストラクト第７巻、第２８７号（第２４
２頁’）（１４２３）には自動生化学的分析器に備えた
サーモスタット付き室が示されており、該室においてサ
ンプル収納管が下方に突出する反応カセットを側板上で
移動させ、サーモスタット付き空気の流れをカセットの
移動に対して垂直方向に流動させる。ガス流によって行
われる比較的遅い温度変化は別にして、この分析器は、
サンプルを作動速度に一致して付加されることができ、
すべてのサンプルが同一処理時間を受けねばならないと
いる欠点を有する。Japanese Patent Abstracts Volume 7, No. 287 (No. 24
2') (1423) shows a thermostatic chamber for an automatic biochemical analyzer, in which a reaction cassette with a sample storage tube protruding downward is moved on a side plate, and a thermostatic air flow perpendicular to the movement of the cassette. Apart from the relatively slow temperature changes made by the gas flow, this analyzer
The sample can be added in accordance with the operating speed,
It has the disadvantage that all samples must undergo the same processing time.
最後に、微生物学的目的用のサンプルを加熱する装置に
おけるミクロタイタープレートの使用はヨーロッパ特許
出願においてミクロタイタープレートは液体によって放
出される熱は送風器によって冷却されるプレート上に配
置させ、電動機によって放出される熱は送風器によって
消散される。Finally, the use of microtiter plates in devices for heating samples for microbiological purposes is described in European patent applications. The heat released is dissipated by the blower.
本発明の実施例により１本発明のさらに他の特徴によっ
て容器がサンプル支持体の縁部により密封体を形成する
縁部を備えた表面を有する場合に取扱い易い装置が得ら
れる。その好適な実施例は縁部が弾性密封材料から作ら
れることにある。Embodiments of the invention provide an easy-to-handle device when the container has a surface with an edge that forms a seal with the edge of the sample support, according to a further feature of the invention. A preferred embodiment thereof consists in that the edges are made of an elastic sealing material.
本発明のさらに他の実施例によれば、サンプル支持体か
存在しない限りにおいてサーモスタット付き液体循環が
維持されず、このような循環は容器がサンプル支持体用
の固着機構を有し、該固着機構は該固着機構がサンプル
支持体を固着するとき弁機構を開放すべく該弁機構と連
結される作動部材を備えることによりサンプル支持体を
取付けた直後に活性化されることが簡単な方法において
備えることができる。According to yet another embodiment of the invention, thermostatic liquid circulation is not maintained insofar as a sample support is present, such circulation being such that the container has an anchoring mechanism for the sample support and the anchoring mechanism is provides in a simple manner that the anchoring mechanism is activated immediately after attaching the sample support by comprising an actuation member coupled to the valve mechanism to open the valve mechanism when the anchoring mechanism anchors the sample support. be able to.
ミクロタイタープレートの異なるセルが等しい温度制御
条件にさらされる高い度合において達成される規則的な
流れは仕切り室への接続がサンプル支持体の中央領域に
おいてなされるとき高められる。それによれば、サンプ
ル支持体の縁部近傍に複数の排出管を有するのが好まし
い。等しい測定において仕切り室のセルを作用させる規
則的な流れを維持するとき、空気を仕切り室から排出さ
せることができるようにするために１本発明のさらに他
の実施例によれば、仕切り室の底部を接続する下方排出
管および底部の上方でかつ関連のサンプル支持体のプレ
ートの下面近傍にそれらの開口を有する上方排出管があ
る。それによれば、サンプル支持体がこれらの下に配置
された仕切り室内で僅かに傾斜するならば特に好都合で
ある。The regular flow achieved to a high degree that the different cells of the microtiter plate are exposed to equal temperature control conditions is enhanced when the connection to the compartment is made in the central region of the sample support. According to this, it is preferable to have a plurality of discharge tubes near the edge of the sample support. According to yet another embodiment of the present invention, in order to allow air to be discharged from the compartment when maintaining a regular flow acting on the cells of the compartment in equal measurements, There is a lower outlet tube connecting the bottom and an upper outlet tube having their opening above the bottom and near the underside of the plate of the associated sample support. Accordingly, it is particularly advantageous if the sample supports are slightly inclined in the compartment arranged below them.
本発明は弁を備えている複数の接続導管に予め定めた温
度の液体を送給するための液体送給装置を含んでおり、
該液体送給装置はサーモスタット付き液体貯水槽と循環
ポンプとを備えている。サンプル支持体を正しい温度に
加熱させるために好都合に使用されるこのような装置は
オランダ特許第１７３，０６６号明細書から公知である
。本発明はかかる装置を簡単化し、それにより複数の接
続点において簡単な手段により適宜な圧力下でかつ正確
な温度によりサーモスタット付き液体が利用し得るよう
にしたことにある。これは本発明によれば、貯水槽内に
循環導管を配設すると共に閉鎖可能な弁機構に接続し、
該導管が貯水槽内に流れ抵抗によって液体を流出するよ
うにすることによって達成される。The invention includes a liquid delivery device for delivering a liquid at a predetermined temperature to a plurality of connecting conduits that are provided with valves;
The liquid supply device includes a thermostatic liquid reservoir and a circulation pump. Such a device, which is advantageously used for heating the sample support to the correct temperature, is known from Dutch Patent No. 173,066. The invention consists in simplifying such a device, thereby making thermostatic liquid available at a plurality of connection points, by simple means, under appropriate pressures and at precise temperatures. According to the invention, this is achieved by arranging a circulation conduit in the water reservoir and connecting it to a closable valve mechanism,
This is accomplished by allowing the conduit to drain liquid through flow resistance into a reservoir.
かかる装置によれば、一方で加熱液体の圧力の影響下で
完全に閉鎖し、他方で比較的小さな力で容易に制御し得
る弁を使用することが重要である。従って、本発明のさ
らに他の実施例において、弁が弁部材を有し、該弁部材
は両端で弁座と協働する密封機構を備え、スリーブ内に
自由に配置され、該スリーブは側部の排出導管を有し、
密封機構間の位置とスリーブの側部の排出導管の位置と
に開口部を有する液体通路を備え、該スリーブは一方の
弁座の一端で液体接続導入管に接続され、他端で制御部
材と協働する。According to such a device, it is important to use a valve that, on the one hand, closes completely under the influence of the pressure of the heated liquid and, on the other hand, can be easily controlled with relatively small forces. Accordingly, in a further embodiment of the invention, the valve has a valve member provided at both ends with a sealing mechanism cooperating with the valve seat and freely disposed within a sleeve, the sleeve having a lateral has a discharge conduit of
a liquid passage having an opening between the sealing mechanism and at the position of the discharge conduit on the side of the sleeve, the sleeve being connected at one end of one valve seat to a liquid connection inlet pipe and at the other end connected to a control member; Collaborate.
以下に本発明を添付図面に示した実施例について説明す
る。The present invention will be described below with reference to embodiments shown in the accompanying drawings.
第１図において符号１は貯水槽を示す。この貯水槽の左
側の上方には６つの容器２が付設され、小さい右側の上
方にはプレート３が設けられ、該プレート３は下方に垂
下する貯水槽１の底部５（第２図参照）に達しない中間
壁４を有している。貯水槽１の左側においてプレート７
は２つの支持ビーム６上に横たわり、プレート７は側部
から中心に向って約１５°の角度で上方に向って傾斜し
ている。In FIG. 1, reference numeral 1 indicates a water tank. Six containers 2 are attached above the left side of this water tank, and a plate 3 is provided above the small right side, which plate 3 is attached to the bottom 5 of the water tank 1 (see FIG. 2) which hangs downward. It has an intermediate wall 4 which does not reach as far. Plate 7 on the left side of water tank 1
lies on two support beams 6, the plates 7 being inclined upwardly from the sides towards the center at an angle of approximately 15°.
このプレート７上には６つのクツション８が取付けられ
、これらのクツション８は容器用の基部としての作用を
なす。これらのクツション８の中心にはパイプ１０が貫
通する孔９が形成されており、パイプ１０は同様にプレ
ート７をも通過する。ホース１１を介してパイプ１０が
弁１３の出力に接続され、弁１３は供給管１４によって
貯水槽１の液体レベル１６の下に配置された主導管は１
５に接続されている。第１図に破線で示したように、主
導管１５はＵ形状であり、ポンプ及びサーモスタット制
御の温度調整装置又はサーモスタット装置１８からの送
り導管１７を有し、ポンプ及びサーモスタット装置１８
はそれ自体公知の型式のものからなっている。Ｕ形状の
主導管１５の端部には流れ抵抗１９が取付けられている
。Mounted on this plate 7 are six cushions 8, which act as bases for the containers. A hole 9 is formed in the center of these cushions 8, through which a pipe 10 passes, and the pipe 10 also passes through the plate 7. A pipe 10 is connected via a hose 11 to the output of a valve 13, which is connected by a supply pipe 14 to a main pipe placed below the liquid level 16 in the reservoir 1.
5. As shown in broken lines in FIG. 1, the main conduit 15 is U-shaped and has a feed conduit 17 from a pump and thermostatically controlled temperature regulating or thermostatic device 18.
are of a type known per se. A flow resistor 19 is attached to the end of the U-shaped main pipe 15.
プレート７には支柱２０が取付けられており。A support 20 is attached to the plate 7.
該支柱２０はそれ自体公知の方法で高さを調整すること
ができ、締付は具２２用の枢軸２１を支持する。The strut 20 can be adjusted in height in a manner known per se, and the clamp supports a pivot 21 for the tool 22.
弾性材料、好ましくは密封気泡を有するエラストマ発泡
材料からなる各クツション８は各クツションの下側に配
置された排出管２３及び高い側に配置された排出管２４
を備えている。排出管２３．２４は上方に出ている小さ
な管を有し、その結果それらの口部はクツション８の表
面上に配置されている。Each cushion 8 made of an elastic material, preferably an elastomeric foam material with sealed cells, has a discharge pipe 23 located on the lower side and a discharge pipe 24 located on the high side of each cushion.
It is equipped with The discharge pipes 23 , 24 have small pipes extending upwardly, so that their mouths are arranged on the surface of the cushion 8 .
各クツション８上には、ミクロタイター（微小滴定量）
プレート２５が配置されており、該ミクロタイタープレ
ート２５は下方に押し下げられた時にその縁部２６が関
連のクツション８上で密封する。On each cushion 8, there is a microtiter (micro titer amount).
A plate 25 is arranged, the microtiter plate 25 sealing with its edge 26 on the associated cushion 8 when pushed downwards.
各ミクロタイタープレート２５上にはカバー２７が配置
され、該カバー２７はその縁部２８によりミクロタイタ
ープレート２５の端縁２６′に対して押圧している。A cover 27 is arranged over each microtiter plate 25, which presses with its edge 28 against the edge 26' of the microtiter plate 25.
締付は具２２は枢軸２１上に幾らか偏心して取付けられ
、それによって締付は具２２をそれらの枢軸上で回転さ
せると、死点を通るので。The clamping tools 22 are mounted somewhat eccentrically on the pivots 21 so that the clamping passes through dead center when rotating the tools 22 on their pivots.
スナップによりミクロタイタープレート２５とカバー２
７を固着する。Micro titer plate 25 and cover 2 are snapped together.
Fix 7.
各ミクロタイタープレート用の２つの締付は具２２の一
方とハンドル２９が接続され、該ハンドル２９は弁１３
の制御ピン３０’ に作用することができる。The two clamps for each microtiter plate are connected to one of the fittings 22 by a handle 29, which is connected to the valve 13.
control pin 30'.
第２図からより明確にされるように、弁１３のピン３０
’は弁部材３０に堅固に接続され、該弁部材３０はそれ
らの間に中央孔を有する閉鎖端面３１および３２を有し
、前記中央孔は４つの開口部３４．３５及び３６によっ
て弁部材３０の外側に接続されている。弁部材３０の下
側には０−リング３７が取付けられ、上側には０−リン
グ３８が取付けられ、それらの０−リング３７．３８は
座３９および４０にそれぞれ協働することができる。As is made clearer from FIG. 2, the pin 30 of the valve 13
' is rigidly connected to a valve member 30, which has closed end faces 31 and 32 with a central hole between them, said central hole being connected to the valve member 30 by four openings 34, 35 and 36. connected to the outside. An O-ring 37 is mounted on the lower side of the valve member 30 and an O-ring 38 is mounted on the upper side, which O-rings 37, 38 can cooperate with seats 39 and 40, respectively.
通常、Ｏ−リング３７は供給管１４内の液圧の影響下で
座３９に係合している。しかし、もし圧力が制御ピン３
０′に働かされた場合に弁部材３０は下方に移動し、開
口部３４は管１４に接続することになり、それにより開
口部３５は排出管１２に接続され、開口部３６は一時的
に弁の上室４１に接続される。この上室４１は３つの幅
広の半径方向通路４２を介してスリーブ４３の外側に接
続されているので、弁部材３０が下方に移動して液体が
上方に代って側方に運ばれる。Normally, the O-ring 37 engages the seat 39 under the influence of hydraulic pressure in the supply tube 14. But if the pressure is on control pin 3
0', the valve member 30 moves downwardly and the opening 34 connects to the pipe 14, thereby connecting the opening 35 to the discharge pipe 12 and opening 36 temporarily It is connected to the upper chamber 41 of the valve. This upper chamber 41 is connected to the outside of the sleeve 43 via three wide radial passages 42, so that the valve member 30 moves downwards and liquid is conveyed to the sides instead of upwards.
液体供給の遮断によりミクロタイタブレートの下方の空
間が開口部３６、上室４１と、排出管１２．ホース１１
及びパイプ１０を介して開口部３５を介して通気され、
その結果液体が容易に排出される。By shutting off the liquid supply, the space below the microtiter plate becomes the opening 36, the upper chamber 41, the discharge pipe 12. Hose 11
and vented through the opening 35 through the pipe 10;
As a result, liquid can be easily drained.
図示の弁は液体の逆圧が克服されるとき実際にはもはや
抵抗力に遭遇せず、その結果酸る型式のスナッナ作用が
得られるという利点がある。The illustrated valve has the advantage that when the liquid counterpressure is overcome, no resistance is actually encountered any longer, resulting in a snapping-type snapping action.
更に弁部材３０はスリーブ４３内の開口部４４内に取付
けられているので、摩擦が発生しない。Furthermore, because the valve member 30 is mounted within the opening 44 in the sleeve 43, no friction occurs.
本発明は、ミクロタイタープレート２５がクツション８
に取付けられ、そして締付は具２２が固着された時に、
正確な温度での液体供給が即座に開始され、それにより
凹部に配置されたサンプルはサーモスタットで調温され
る液体のまわりの流れに従わされ、かつ迅速に正確な温
度に達するという利点がある。In the present invention, the microtiter plate 25 is attached to the cushion 8.
and is tightened when the fixture 22 is secured.
The advantage is that the liquid supply at the correct temperature starts immediately, so that the sample placed in the recess is forced to follow the flow around the thermostatically controlled liquid and quickly reaches the correct temperature.
本発明は、本装置内にミクロタイタープレートを容易に
適用させることができ、カバーによってサンプルを遮蔽
でき、研究するために発生されるべき微生物学的、酵素
学的又は免疫学的方法に損害を与える局部的な高温を発
生させないでサンプルを迅速に所望の温度にさせること
ができる。The present invention allows a microtiter plate to be easily applied within the device, shielding the sample by a cover and preventing damage to the microbiological, enzymatic or immunological methods to be generated for research. The sample can be quickly brought to the desired temperature without causing localized high temperatures.
本発明は仕切り室内の液体が僅かに加圧されているので
、ミクロタイタープレートが機械的手段によって固着さ
れねばならない装置に基づいて上述したけれども、吸上
げによって仕切り室を通る液体流を生じさせることによ
り、サンプル支持体を副圧力によってクツション上に押
し下げることもできる。Although the invention has been described above on the basis of a device in which the microtiter plate has to be secured by mechanical means, since the liquid in the compartment is slightly pressurized, it is also possible to create a liquid flow through the compartment by suction. This also allows the sample support to be pressed down onto the cushion by means of secondary pressure.
図は本発明のサンプル支持体を所定の温度に維持する装
置の実施例を示したもので、第１図はその平面図、第２
図は第１図の■−■線の断面図、第３図はサンプル支持
体の詳細図である。
図中、符号１は貯水槽、２は容器、１０はパイプ、１１
はホース、１２は排出管、１３は弁。
３０は弁部材、１４は供給管、１５は主導管、１８はポ
ンプ、１９は流れ抵抗、２３．２４は排出管、２５はサ
ンプル支持体、２６は縁部、２９は作動部材、３４．３
５．３６は開口部、３７．３８は密封部材、３９．４０
は弁座である。The figures show an embodiment of the apparatus for maintaining a sample support at a predetermined temperature according to the present invention.
The figure is a sectional view taken along the line ■--■ in FIG. 1, and FIG. 3 is a detailed view of the sample support. In the figure, 1 is a water tank, 2 is a container, 10 is a pipe, 11
is a hose, 12 is a discharge pipe, and 13 is a valve. 30 is a valve member, 14 is a supply pipe, 15 is a main pipe, 18 is a pump, 19 is a flow resistance, 23.24 is a discharge pipe, 25 is a sample support, 26 is an edge, 29 is an actuation member, 34.3
5.36 is the opening, 37.38 is the sealing member, 39.40
is the valve seat.
Claims (13)
するプレートから成るサンプル支持体を収容するための
１つ又はそれ以上の容器と、貯水槽中において熱支持液
体を所定の温度に維持する温度制御装置と、貯水槽と容
器との間を接続させた導管機構とを備え、容器には容器
とサンプル支持体を形成するプレートとの間に密封仕切
り室を形成するための密封機構を備え、導管機構には、
貯水槽から仕切り室までの接続導管と、仕切り室から貯
水槽への排出管とを設け、該接続導管と排出管の少なく
とも１つに密閉可能な弁機構を備えたことを特徴とする
サンプル支持体を所定の温度に維持する装置。(1) one or more containers for containing a sample support consisting of a plate with multiple recesses, such as a microtiter plate, and a temperature for maintaining a thermal support liquid at a predetermined temperature in a water reservoir; a control device and a conduit mechanism connecting between the reservoir and the container, the container having a sealing mechanism for forming a sealed compartment between the container and the plate forming the sample support; The conduit mechanism includes
A sample support comprising a connecting conduit from the water storage tank to the partition chamber and a discharge pipe from the partition chamber to the water storage tank, and at least one of the connection conduit and the discharge tube is equipped with a sealable valve mechanism. A device that maintains the body at a specified temperature.
る縁部を備えた表面を設けたことを特徴とする特許請求
の範囲第１項に記載の装置。2. Device according to claim 1, characterized in that the container is provided with a surface with an edge forming a seal with the edge of the sample support.
とを特徴とする特許請求の範囲第２項に記載の装置。3. A device according to claim 2, characterized in that the surface with the edges is formed from an elastic sealing material.
固着機構には該固着機構がサンプル支持体を固着すると
き、弁機構を開放すべく該弁機構と連結させた作動部材
を備えたことを特徴とする特許請求の範囲第１項乃至第
３項のいずれか１項に記載の装置。(4) The container is provided with a fixing mechanism for the sample support, and the fixing mechanism includes an actuating member connected to the valve mechanism to open the valve mechanism when the fixing mechanism fixes the sample support. An apparatus according to any one of claims 1 to 3, characterized in that:
徴とする特許請求の範囲第１項乃至第４項のいずれか１
項に記載の装置。(5) Any one of claims 1 to 4, characterized in that the connecting conduit is connected to the central area of the container.
Equipment described in Section.
を特徴とする特許請求の範囲第１項乃至第５項のいずれ
か１項に記載の装置。(6) The device according to any one of claims 1 to 5, characterized in that a plurality of discharge pipes are arranged near the edge of the container.
近傍に配置させたことを特徴とする特許請求の範囲第６
項に記載の装置。(7) Claim 6, characterized in that the partition chamber is rectangular and the discharge pipe is arranged near the corner of the partition chamber.
Equipment described in Section.
２３）と該底部の上方で且つ関連のサンプル支持体のプ
レートの下面近傍にそれらの開口部有する上方排出管と
から構成したことを特徴とする特許請求の範囲第６項又
は第７項に記載の装置。(8) Lower discharge pipe connecting the bottom of the partition chamber with the discharge pipe (
23) and an upper discharge tube having an opening thereof above the bottom and near the underside of the plate of the associated sample support. equipment.
る特許請求の範囲第１項乃至第８項のいずれか１項に記
載の装置。(9) The device according to any one of claims 1 to 8, characterized in that the container is inclined at an angle of about 5 to 20 degrees.
を特徴とする特許請求の範囲第８項または第９項に記載
の装置。(10) The device according to claim 8 or 9, wherein the upper discharge pipe is arranged higher than the container.
を備え、該貯水槽には弁機構を備えている複数の接続導
管に予め定めた温度の液体を送給するための液体送給装
置を設け、貯水槽内に主導管を配置されると共に閉鎖可
能な接続導管と接続させ、該主導管が流れ抵抗によって
貯水槽内に液体を流出することを特徴とする特許請求の
範囲第１項に記載の装置。(11) a water storage tank having a thermostat and a circulation pump; the water storage tank is provided with a liquid feeding device for feeding a liquid at a predetermined temperature to a plurality of connecting conduits each having a valve mechanism; 2. A main conduit according to claim 1, characterized in that a main conduit is arranged in the water reservoir and connected to a closable connecting conduit, which main conduit drains the liquid into the water reservoir due to flow resistance. Device.
弁座と協働する密封機構を備え、側部の排出導管を有す
るスリーブ内に弁部材を自由に配置させ、弁部材には密
封機構間の位置とスリーブの側部の排出導管の位置とに
開口部を有する液体通路を備え、一方の弁座の一端でス
リーブを液体接続導管に接続させ、他端においては制御
部材と協働させたことを特徴とする特許請求の範囲第１
１項に記載の装置。(12) The valve mechanism is provided with a valve member, the valve member being provided with a sealing mechanism cooperating with the valve seat at both ends, the valve member being freely disposed within a sleeve having side discharge conduits; is provided with a fluid passageway having an opening between the sealing mechanism and at the location of the discharge conduit on the side of the sleeve, connecting the sleeve to the fluid connection conduit at one end of the valve seat and at the other end of the control member. Claim 1 characterized in that the first claim is made in cooperation with
The device according to item 1.
する密封機構を備え、側部の排出導管を有するスリーブ
内に弁部材を自由に配置させ、弁部材には密封機構間の
位置とスリーブの側部の排出導管の位置に開口部を有す
る液体通路を備え、スリーブは一方の弁座の一端で液体
接続に接続され、他端で制御部材と協働することを特徴
とする弁。(13) having a valve member, the valve member having a sealing mechanism cooperating with the valve seat at each end, the valve member being freely disposed within a sleeve having a side discharge conduit; The sleeve is provided with a liquid passage having an opening at the location between the mechanisms and at the location of the discharge conduit on the side of the sleeve, the sleeve being connected to a fluid connection at one end of one valve seat and cooperating with a control member at the other end. Features a valve.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL8801127 | 1988-04-29 | ||
| NL8801127A NL8801127A (en) | 1988-04-29 | 1988-04-29 | DEVICE FOR BRINGING AND HOLDING A SAMPLE HOLDER TO A PARTICULAR TEMPERATURE. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0216430A true JPH0216430A (en) | 1990-01-19 |
Family
ID=19852229
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1111924A Pending JPH0216430A (en) | 1988-04-29 | 1989-04-28 | Apparatus for maintaining sample support at specified temperature |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0339710B1 (en) |
| JP (1) | JPH0216430A (en) |
| AT (1) | ATE79307T1 (en) |
| DE (1) | DE68902414T2 (en) |
| DK (1) | DK208589A (en) |
| ES (1) | ES2035523T3 (en) |
| FI (1) | FI891976A (en) |
| NL (1) | NL8801127A (en) |
| NO (1) | NO891796L (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2678782B1 (en) * | 1991-07-04 | 1995-06-30 | Jaeger | CONNECTION DEVICE BETWEEN A FLEXIBLE ELECTRIC CIRCUIT AND A SECOND CIRCUIT. |
| DE19521947C1 (en) * | 1995-06-16 | 1996-08-22 | Medipro Medizinische Diagnosti | Vibrating water bath assembly with at least two individually temp.-controlled positions |
| DE29714206U1 (en) * | 1997-08-08 | 1997-12-11 | Hoechst Marion Roussel Deutschland GmbH, 65929 Frankfurt | Pipetting robot with improved temperature control device |
| ATE270151T1 (en) | 1997-08-08 | 2004-07-15 | Aventis Pharma Gmbh | PIPETTING ROBOT WITH TEMPERATURE DEVICE |
| DE10046224A1 (en) * | 2000-09-19 | 2002-03-28 | Merck Patent Gmbh | Unit for parallel incubation of solutions includes frame holding titration plate closely against cover plate, forming tight seal |
| DE102009015869B4 (en) | 2009-04-01 | 2011-03-03 | Schneckenburger, Herbert, Prof. Dr. | Microtiter plate with heating device |
| CN101632948B (en) * | 2009-09-08 | 2011-06-15 | 苏州培英实验设备有限公司 | TSHY-10 gradient water-bathing constant temperature oscillator |
| CN110115963B (en) * | 2019-04-18 | 2020-12-22 | 通用生物系统（安徽）有限公司 | Preparation method of coupling activator for primer synthesis |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3505023A (en) * | 1967-11-13 | 1970-04-07 | Research Corp | Thermally conditioned spot plate |
| US3634651A (en) * | 1970-12-04 | 1972-01-11 | Becton Dickinson Co | Serological incubator |
| DE3115745C2 (en) * | 1981-04-18 | 1983-03-17 | Kernforschungsanlage Jülich GmbH, 5170 Jülich | Method and device for the serial cultivation of microorganisms |
| JPS58154661A (en) * | 1981-10-20 | 1983-09-14 | Toshiba Corp | Thermostatic chamber of automatic biochemical analizer |
| JPS5930038A (en) * | 1982-08-13 | 1984-02-17 | Toshiba Corp | Thermoregulator |
| US4775003A (en) * | 1987-03-27 | 1988-10-04 | Koh John K | Laboratory materials and apparatus support and laminar flow thermal transfer device |
-
1988
- 1988-04-29 NL NL8801127A patent/NL8801127A/en not_active Application Discontinuation
-
1989
- 1989-04-14 AT AT89200936T patent/ATE79307T1/en not_active IP Right Cessation
- 1989-04-14 ES ES198989200936T patent/ES2035523T3/en not_active Expired - Lifetime
- 1989-04-14 EP EP89200936A patent/EP0339710B1/en not_active Expired - Lifetime
- 1989-04-14 DE DE8989200936T patent/DE68902414T2/en not_active Expired - Fee Related
- 1989-04-26 FI FI891976A patent/FI891976A/en not_active IP Right Cessation
- 1989-04-28 NO NO89891796A patent/NO891796L/en unknown
- 1989-04-28 JP JP1111924A patent/JPH0216430A/en active Pending
- 1989-04-28 DK DK208589A patent/DK208589A/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| ES2035523T3 (en) | 1993-04-16 |
| DE68902414D1 (en) | 1992-09-17 |
| NO891796L (en) | 1989-10-30 |
| EP0339710A3 (en) | 1990-05-30 |
| ATE79307T1 (en) | 1992-08-15 |
| FI891976A0 (en) | 1989-04-26 |
| DK208589D0 (en) | 1989-04-28 |
| FI891976A (en) | 1989-10-30 |
| DE68902414T2 (en) | 1993-03-25 |
| EP0339710B1 (en) | 1992-08-12 |
| EP0339710A2 (en) | 1989-11-02 |
| NO891796D0 (en) | 1989-04-28 |
| NL8801127A (en) | 1989-11-16 |
| DK208589A (en) | 1989-10-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US3801077A (en) | Humidifying apparatus | |
| JPH0216430A (en) | Apparatus for maintaining sample support at specified temperature | |
| FI84854B (en) | KOPPLANDE MINI-ELVENTIL. | |
| KR101019723B1 (en) | Flow adjusting valve | |
| US4995419A (en) | Mixing valve for single-lever faucet | |
| DE69534321D1 (en) | VALVE FOR A HEAT TRANSFER SYSTEM | |
| KR920004953B1 (en) | Method and apparatus for evacuating and filling heat pipes andsimilar closed vessels | |
| US20010026776A1 (en) | Methods and apparatus for improved fluid control utilizing a U-valve employing a bidirectional check valve | |
| US2940474A (en) | Multi-port valves | |
| US5121642A (en) | Liquid sampling method and apparatus | |
| JPH09506932A (en) | Equipment for heat treatment of thin film wafers | |
| US3884260A (en) | Diaphragm valve apparatus and control systems employing such valve apparatus | |
| US3145926A (en) | High pressure colk air-hot air mixing box for a ventilating, heating and air conditioning system | |
| JPH0442913Y2 (en) | ||
| US2043074A (en) | Steam trap | |
| US3782335A (en) | Heat exchanger for liquid at overpressure | |
| US5621163A (en) | Apparatus for testing resin flow | |
| US5733775A (en) | Temperature control device | |
| US2868219A (en) | Valve construction | |
| JP3024066B2 (en) | Method and apparatus for selecting and distributing fluid | |
| US634547A (en) | Steam heating apparatus and device for controlling action thereof. | |
| SU1182317A1 (en) | Device for sampling microbiological media | |
| JPH11143551A (en) | Pipet robot having improved temperature controller | |
| US3795256A (en) | Tank with integral remotely controlled power actuated bottom valve | |
| JP2506699B2 (en) | Air trap valve for liquids |