JPH02159549A - Density measuring apparatus - Google Patents

Abstract

PURPOSE:To enable elimination of a danger of infection by germ even when measurement of a body liquor is performed by arranging a casing, a density measuring electrode and an opening for spotting. CONSTITUTION:A long-sized sheet 1 is provided with openings 20 for spotting at a specified interval and a diffusion limiting film holder provided with a diffusion limiting film covering the openings is housed at a specified position of a casing 2 in such a manner as to be movable in a specified direction to spot a liquid to be measured at the openings 20. Then, the holder is moved by a specified distance toward the openings 20 to bring a density measuring electrode 22 into contact with the diffusion limiting film thereby enabling measurement of a density of material to be measured. This not only eliminates work of detaching the diffusion limiting film but also separates a location where a solution to be measured is spotted from a location where measurement of a density is performed with the electrode 22 thereby enabling the elimination of a danger of infection by germ.

Description

[Detailed Description of the Invention] <Industrial Application Field> The present invention relates to a concentration measuring device, and more specifically,
When diffusing a target substance through a diffusion-limiting membrane and measuring the concentration based on the diffused target substance,
The present invention relates to a novel concentration measuring device in which a spotting position of a solution to be measured is different from a measurement position based on the spotted solution to be measured.

<Conventional technology> Focusing on the characteristic of being able to selectively detect extremely complex organic compounds, proteins, etc. with extremely high sensitivity, bioactive substances are immobilized on the surface of an electrode. Research and development is being carried out to determine the apj of the above-mentioned organic compounds, proteins, etc. using enzyme electrodes.

When measuring a target substance using the enzyme electrode, the target substance is usually oxidized, reduced, etc. in the presence of the physiologically active substance to reduce the amount of produced substances or disappeared substances. Since the concentration of the substance to be measured is determined by measurement, the upper limit of the measurable concentration is limited by the amount of a substance that causes oxidation, reduction, etc., such as oxygen, to exist.

Focusing on this point, attempts have been made to increase the measurement limit concentration by attaching a diffusion-limiting membrane to the surface side of the immobilized enzyme membrane on which the physiologically active substance is immobilized, and by limiting the permeation rate of the substance to be measured. .

Specifically, a diffusion-limiting membrane is attached to a cap that is screwed onto the base of a rod-shaped enzyme electrode, and screwing in the cap automatically brings the diffusion-limiting membrane into close contact with the immobilized enzyme membrane. There is.

By employing such a configuration, the diffusion-limiting membrane can limit the permeation rate of the nj target substance, making it possible to measure up to a considerably high concentration. In addition, the diffusion-limiting membrane is made removable in order to eliminate the influence of interfering substances contained in the solution to be measured (for example, an increase in the diffusion-limiting effect due to adhesion of interfering substances) and to perform a good measurement. Therefore, a screw-in mechanism using a cap is adopted.

<Problems to be Solved by the Invention> When the diffusion-limiting membrane holder having the above structure is adopted, the diffusion-limiting membrane can be replaced relatively easily, but clogging due to use may occur. In order to eliminate this problem, the diffusion-limiting membrane must be replaced more frequently, which makes the concentration measurement work considerably more complicated, especially when measuring the concentration of substances contained in body fluids. However, there is a problem in that there is a risk of disease infection due to pathogenic bacteria remaining on the surface of the concentration measuring electrode.

Furthermore, if the enzyme electrode base becomes small in diameter and the cap becomes small as a result, manual operation becomes extremely difficult, and the problem of complicating regular operations becomes even more pronounced.

Taking these problems into consideration, we have adopted a diffusion-limiting membrane holder that has an opening formed at a predetermined position in a thin plate material and a diffusion-limiting membrane pasted to cover one side of the opening. A solution containing the target substance to be measured (hereinafter referred to as the target solution) is dropped into the holder in advance, and the concentration of the test solution can be measured with the diffusion-limiting membrane pressed against the immobilized enzyme membrane. However, thin plate materials usually have no stiffness, which makes it difficult to drip the solution to be measured and attach it to the measuring device itself, and it is necessary to replace the diffusion limiting membrane holder quite often. Therefore, there is a problem that the concentration measurement work becomes complicated as a whole. In addition, since the above-mentioned diffusion-limiting membrane may become clogged with use, it is preferable to
Each time the concentration ρj is determined, it must be discarded and a new thin plate with a diffusion-limiting membrane attached must be used.
More thin plates than necessary will be discarded, which is uneconomical, and it will also be necessary to dispose of the thin plates and take out new ones. There is a problem in that the operation becomes extremely complicated.

To explain in more detail how the operation becomes complicated, ■ Turn on the 1lPI constant water device power switch, and
Open the cover of the measuring device, ■ Unseal and take out the thin plate material to which the diffusion-limiting membrane is attached, ■ Drop the solution to be measured onto the thin plate material. If the solution to be measured is blood, collect the blood, and then ■ Insert the thin plate into the measuring device. ■ Bring the diffusion-limiting membrane attached to the inserted thin plate into close contact with the surface of the electrode for concentration measurement. In this state, measure the substance to be measured. Measure concentration.

■ After that, release the tightness of the diffusion limiting membrane against the electrode surface, ■ Remove the thin plate material from the Δ-j constant device, ■ Close the cover of the Δ-1 constant device, and discard the extracted thin plate material. ■ Open the power switch of the measuring device.

This requires a series of operations, and since there are some parts that are difficult to operate, the concentration measurement work as a whole becomes extremely complicated.

Furthermore, since the above does not mention at all the calibration work based on standard solutions with known concentrations, the necessary work becomes even more complicated as the calibration work is actually required. Become.

In addition, in the above, the concentration of the substance to be measured is measured using an enzyme electrode.
Although only the case where P1 is determined has been described, a similar problem occurs when measuring the concentration of the substance to be measured using an electrode other than the enzyme electrode.

<Object of the invention> This invention has been made in view of the above problems.
It is an object of the present invention to provide a concentration measuring device that can significantly reduce the frequency of mounting and discarding a diffusion limiting membrane, and can also significantly simplify concentration measuring work.

Means for Solving the Problems> In order to achieve the above object, the concentration measuring device of the present invention has a thin plate having openings for passing the solution to be measured at predetermined intervals, and each opening A concentration measuring electrode is provided at a predetermined position of a casing that accommodates a diffusion-limiting membrane holder, which is movably in a predetermined direction, and is provided with a diffusion-limiting membrane so as to cover it, and a solution point corresponding to the opening is provided on the upper surface of the casing. The contact portion and the δ1j constant portion that brings the concentration measuring electrode into contact with the diffusion limiting film are arranged at a predetermined distance apart in the sheet moving direction. However, the above-mentioned thin sheet may be a long sheet or a disc-shaped sheet, but in the case of a long sheet, it is movable in the longitudinal direction, and a disc-shaped sheet is used. In the case of a sheet, it is sufficient if it is movable in the direction of rotation.

Although a sheet drive mechanism for moving the thin sheet in only one direction may be provided, a sheet drive mechanism for reciprocating the thin sheet in a predetermined direction may also be provided.

Further, the solution spotting section has a large diameter opening that is larger than the opening for passing the solution to be measured, and the solution spotting section and the measurement section are separated by the distance between the openings for passing the solution to be measured. Preferably.

However, it may be a simple structure in which no opening is specifically formed and the diffusion-limiting membrane holder is exposed over a predetermined range.

Furthermore, when the solution spotting part is moved a predetermined distance MM, 2t!
! It is preferable that a cover is provided at location f and selectively covers any of the solution spotting portions. In this case, it is more preferable that a small diameter opening is formed at a predetermined position of the cover so as to be able to directly face the opening for passage of the ΔN constant target solution exposed through one of the large diameter openings.

Furthermore, the solution spotting section is placed at a distance corresponding to the distance between the openings for transmitting the substance to be measured.
It is preferable that a light-emitting element is provided at a location below the elongated sheet so as to directly face one of the solution spotting parts.

In the concentration measuring device with the above configuration, openings are formed in the thin sheet at predetermined intervals for passage of the solution to be measured, and a diffusion limiting film is provided to cover each opening. A restriction membrane holder is housed in a predetermined position in the casing of the concentration measuring device so as to be movable in a predetermined direction, and the solution to be measured is applied to the opening through the solution spotting part, and then the diffusion restriction membrane holder is opened. By moving the concentration measuring electrode a predetermined distance in the formation direction and bringing it into contact with the diffusion limiting film,
The concentration of the substance to be measured contained in the solution to be measured can be measured.

Therefore, while performing measurements equal to the number of diffusion-limiting membranes installed in the diffusion-limiting membrane holder, not only is it unnecessary to attach and detach the diffusion-limiting membranes, but also the concentration measuring electrode is Since the location where the concentration measurement is performed is far away, the risk of disease infection can be eliminated even when measuring body fluids.

If the thin sheet is a long sheet, the above effect can be achieved by moving the long sheet in the elongated direction, and if the thin sheet is a disc-shaped sheet, the above effect can be achieved by moving the long sheet in the elongated direction. The above effect can be achieved by rotating the disk-shaped sheet and moving the diffusion-limiting membrane.

Furthermore, if a sheet drive mechanism for moving the thin sheet in only one direction is provided, the thin sheet can be moved in one direction without touching the thin sheet. On the other hand, if a sheet drive mechanism is provided that reciprocates the thin sheet in a predetermined direction, the solution is applied in advance to two openings in the thin sheet, and then the backward and forward movements are performed. By performing the steps in this order, the order in which the density n1 determination is performed can be reversed.

Therefore, for example, first, a DJ constant target solution of unknown concentration is applied, and then a DJ target solution of unknown concentration is applied. It is possible to spot the #1 constant target solution and measure the concentrations of the DI constant target solution whose concentration is known and the iDJ constant target solution whose concentration is unknown in this order.

Furthermore, the solution spotting section has a large diameter opening that is larger than the opening for passing the solution to be measured, and the solution spotting section and the measurement section are separated by the distance between the openings for passing the solution to be measured. If the solution is spotted, the solution can be easily spotted through the solution spotting section, and the concentration can be measured by simply moving the diffusion limiting membrane holder by the distance between the openings for passing the solution to be measured after spotting the solution. In addition to being able to perform measurement operations, it is also possible to perform the next solution spotting operation.

Furthermore, when the solution spotting part is separated by a predetermined distance,
If a cover is provided to selectively cover either solution spotting section, the solution spotting section for dispensing a solution of unknown concentration and the solution spotting section for dispensing a solution of known concentration are provided. The solution spotting part to be spotted can be separated in advance,
A calibration operation based on a solution with a known concentration and a concentration DJ constant operation based on a solution with an unknown concentration can be reliably performed. In this case, if a small-diameter opening is formed at a predetermined position of the cover so that it can directly face the opening for passage of the n1 constant target solution exposed through one large-diameter opening, the concentration is unknown depending on the state of the cover. You can choose between a state in which only a solution of 100% is applied, and a state in which a solution with a known concentration and a solution with an unknown concentration can be applied. If the latter state is selected, both hot liquids can be applied At the same time, by moving the thin sheet back and forth, the calibration operation and concentration A
The e1 constant operations can be performed in this order.

Further, the solution spotting portions are provided at two locations separated by a distance corresponding to the distance between the openings for transmitting the substance to be measured, and are placed in a long length directly facing one of the solution spotting portions. When a light emitting element is provided below the sheet, it is possible to apply solutions to the openings for transmitting each target substance through both hot liquid spotting parts, and also to apply a target solution with a known concentration. It is possible to clearly identify the solution spotting part to which the solution should be applied and the solution spotting part to which the solution to be measured whose concentration is unknown should be dropped.

<Example> Hereinafter, embodiments will be described in detail with reference to the accompanying drawings showing examples.

FIG. 1 is a partially cutaway perspective view showing an embodiment of the concentration measuring device of the present invention, and FIG. 2 is a vertical sectional view, in which a long sheet ( A space for accommodating 1) is formed. A spotting opening (20) is formed at a predetermined position upstream of the running path of the long sheet (1), and a storage solution is stored above a measurement position (21a) formed at a predetermined position downstream. A tank (21) is arranged. An electrode lifting mechanism (3) is arranged to selectively raise the concentration measuring electrode (22) to a position where it comes into close contact with the long sheet (1) and a position where it comes into close contact with the storage solution storage tank (21). . Furthermore, holes for positioning the long sheet (1) (
13) (13a) A pair of sensors (24) and (25) are arranged at predetermined positions that can directly face the end detection hole (14). The pair of sensors (24) and (25) are
This is a reflective photosensor arranged so that it can be operated simultaneously only when a hole (14) for end detection is present, and cannot be operated simultaneously in other cases. In addition, (26) is a motor for raising and lowering the concentration 813 constant electrode (22) and feeding the long sheet (1). It has a processing section (not shown) that performs processing and generates concentration data, and a control section (not shown) that drives the motor (26).

FIG. 3 is a perspective view of the diffusion restriction holder, in which openings (15) for passing the solution to be measured and holes for passing the concentration measuring electrodes are provided at predetermined intervals in the center of a long sheet (1) of a predetermined width. A large diameter opening (15a) is formed, and each opening (1
A diffusion-limiting film (16) is attached to the lower surface of the long sheet (1) so as to cover the film (5). Then, the terminal opening (I5
) and the large-diameter opening (15a), position detection holes (13) (13a) are formed in a predetermined positional relationship with respect to the opening (15) at the end. A hole (14) for end detection is formed depending on the position. Note that the long sheet (1) may be made of any material as long as it has resistance to the test solution. In addition, the above diffusion limiting film (1G)
The main function is to separate interfering substances such as blood cells, for example, a polycarbonate membrane with many micropores, and the concentration measuring electrode (22), which will be described later, is equipped with a diffusion-limiting membrane that restricts the permeation of the substance to be measured. In this case, clogging of the diffusion limiting film attached to the concentration measuring electrode (22) can be prevented. However, it is also possible to adopt a configuration in which a film capable of restricting the permeation of the substance to be measured is used as the diffusion-limiting membrane (lG), and no diffusion-limiting membrane is attached to the concentration measuring electrode (22). In order to eliminate the decrease in measurement accuracy caused by clogging, a diffusion-limiting membrane (1B) whose main function is to separate interfering substances is used as a long sheet (1).
It is preferable to attach a diffusion-limiting membrane to the concentration n1 constant electrode (22) to limit the permeation of the target substance. Furthermore, it is preferable that numbers for displaying the remaining amount of the diffusion limiting film (1B) are printed near the opening (15).

FIG. 4 is a partially cutaway perspective view showing the state in which the long sheet (1) is stored in the cartridge, and shows the supply section (7) in which the long sheet (1) is wound in advance, and the long sheet (1). A winding section (18) that winds up the material into a roll, and a bridge section (1) that integrally connects the supply section (17) and the winding section (18).
9). An opening 00) is formed in the center of the bridge portion (19). Further, the winding shaft (18a) provided on the winding section (18) is made to protrude outside the cartridge (11), and the winding shaft (18a) is provided on the winding section (18).
8), the rotational force is transmitted.

The electrode lifting mechanism (3) for lifting and lowering the concentration 71111 constant electrode (22) is as shown in FIGS. 5A to 5D.
It also serves as a mechanism for feeding the long sheet (1), and has a two-system rotational force transmission mechanism (31) (41) with a one-way clutch interposed in order to synchronize the lifting and lowering operations and the feeding operation. )have.

Rotational force transmission mechanism (31) for feeding the long sheet (1)
is a disk (32) that is rotated forward and backward by a motor (2B).
) is provided with an engagement pin (33) at a predetermined position, and a cam (34) that engages with the engagement pin (33) and rotates as the disk (32) rotates by a predetermined angle.
is provided. The shaft of the cam (34) and the winding shaft (Ilia) are arranged concentrically, and the long sheet (1) is wound around both wheels so as to transmit rotational force only in the winding direction. A one-way clutch (35) made of a coil spring is provided.

The configuration of the rotational force transmission mechanism (41) for raising and lowering the concentration measuring electrode (22) is as explained below.

A cylindrical protruding shaft (43) is provided in the center of a disk (42) that is rotated forward and backward by a motor (26), and a cylindrical protrusion (44) concentric with the protruding shaft (43) is provided. It is being Then, a protruding shaft (45) having the same diameter as the above-mentioned protruding shaft (43) is provided.
) and a cylindrical protrusion (46) having the same diameter as the cylindrical protrusion (44) are arranged integrally with each other and directly opposite the protruding shaft (43) and the cylindrical protrusion (44). At the same time, the outer periphery of the double-protruding shaft (43) (45) and the cylindrical protrusion (44)
One-way clutches (47) and (48) each made of a coil spring are provided on the outer periphery of (4G).

The one-way clutches (47) and (48) transmit rotational force in different directions, and a regulating cylinder (49) is disposed around the outer periphery of the one-way clutch (48). This regulating cylinder (49) has a notch (49a) formed at a predetermined position that is connected to the one-way clutch (48).
A flange (49b) formed in a predetermined range on the outer peripheral surface of the regulating cylinder (49) rotates following the one-way clutch (48) by being engaged with one end of a coil spring constituting the regulating cylinder (49). engages with a regulating protrusion (49c) provided at a predetermined position on the main body of the measuring device, thereby canceling rotational force transmission by the one-way clutch (48).

Furthermore, a rotary cam (50
) are connected. This rotary cam (50) has a small diameter cam portion (50b) and a gradually increasing radius cam portion (50c) in the range determined based on the formation range of the flange (49b), and the remaining range is a large diameter cam portion (50d). and,
The rotating cam (50) operates a pantograph mechanism (51) that raises and lowers the concentration measuring electrode (22). The pantograph mechanism (51) includes two pairs of units (51b) (51c) rotatably connected in an X-shape to a pair of bases (51a) separated by a predetermined distance.
and two pairs of single units (51b) (51c
) has a stand (51d), and also has an engaging protrusion (51e) at a predetermined position of one unit (51b) that engages with the rotating cam (50). There is.

Furthermore, the single body (51b) is rotatably and slidably engaged with the support base (51d), and
The unit (51c) is rotatably and slidably engaged with the base (51a), and both units (51c) are rotatably and slidably engaged with the base (51a).
1b) A tension spring (51f) is provided between the lower parts of (51c).

The long sheet (1) with the above configuration is placed in a cartridge (11).
and the casing of the concentration 71111 constant device (
2, the concentration of the target substance in the test solution can be measured as follows. However, FIG. 6 is a timing chart showing the operation of each part.

In a state where no work necessary for concentration measurement is performed, both sensors (24) (25) are connected to the position detection holes (13) (13a) (
Since the sensor 14) is directly facing a location where the sensor 14) is not formed, an ON signal is output from both sensors (24) and (25), and the leading edge of the long sheet (1) is detected. However, in this state, the concentration measuring electrode (22) has an oval large diameter opening (
15a) and is brought into contact with the opening of the storage solution storage tank (21).

In this state, the control unit controls the motor (2G
) to lower the concentration measuring electrode (22), and then feed the long sheet (1). That is, the motor (26) is rotated in the normal direction, and the one-way clutch (48) is operated until the flange (49b) of the regulating cylinder (49) engages with the regulating protrusion (49c).
The concentration measuring electrode (22) is retracted by transmitting rotational force and rotating the rotary cam (50).

Then, when the retracting operation is completed, the engagement pin (33) and the cam (34) engage, so that the one-way clutch (35) then transmits the rotational force to the winding shaft (18a). , a long sheet (1) can be sent.

In addition, while the long sheet (1) is being fed, the regulating cylinder (4
9) is prevented from rotating, so the rotary cam (50) remains stopped.

Then, on condition that one sensor (24) outputs an ON signal and the other sensor (25) outputs an OFF signal, the motor (26) generates a long sheet (
1) Stop feeding. At this point, the opening (15) of the long sheet (1) directly faces the spotting opening (20), and the large diameter opening (15a) directly faces the concentration measuring electrode (22). Therefore, in this state, by reversing the motor (2B), the other one-way clutch (47)
The rotational force is transmitted to the rotating cam (50) through the
1 constant electrode (22) can be raised.

Next, an opening (15) located above the spotting opening (20) is opened.
) After dropping the test solution, when the first n1 constant switch operation is performed, the motor (28) first rotates forward and lowers the concentration measuring electrode (22), then lowers the long sheet (1). By sending the membrane a predetermined distance, the diffusion limiting membrane (16) is brought directly opposite the concentration measuring electrode (22). In this state, the sensor (24) outputs an OFF signal, and the sensor (24) outputs an OFF signal.
25) outputs an ON signal.

Next, on condition that the concentration measuring electrode (22) has been refreshed and is ready for measurement, the motor (
2B) is reversed for a short period of time, the concentration measuring electrode (22) is slightly raised and brought into contact with the diffusion limiting membrane (IB), thereby measuring the concentration of the target substance contained in the test solution. Thereafter, the motor (2B) is rotated in the normal direction to lower the concentration measuring electrode (22), and then the long sheet (1) is sent a predetermined distance, and then the motor (26) is rotated in the reverse direction to lower the concentration measuring electrode (22). ) to rise. In this state, the sensor (24) is outputting an ON signal again, and the sensor (25) is outputting an OFF signal.

Thereafter, by performing the series of operations described above, the concentration of the target substance contained in the test solution or the concentration of the target substance contained in the standard solution can be measured.

As a result of feeding the long sheet (1), the sensor (24
) If the holes (13a) and (14) are detected by (25), it is the last diffusion-limiting membrane (1B), so 1
The operation after performing the concentration measurement operation once is blocked, and if necessary, it is displayed that the concentration measurement has been performed by all the diffusion restriction membranes (16). Therefore, from the casing C) of the concentration JI11 constant device to the cartridge (
By taking out and discarding the elongated sheet (1) together with 11) and storing a new elongated sheet (1), the concentration measurement operation can be performed a plurality of times again.

As is clear from the above explanation, the spotting position of the measurement target solution and the concentration measurement position with the concentration measuring electrode are different, so even when the measurement target solution is a body fluid, infection by diseases caused by pathogenic bacteria etc. can be prevented. It can definitely be prevented.

<Example 2> Fig. 7 is a partially cutaway perspective view showing another example of the concentration measuring device, and Fig. 8 is a longitudinal cross-sectional view. Two dotting openings in the direction of movement (2
0a> (20b) and the long sheet (
The only difference is that a motor (27) for rewinding 1) and a drive force transmission mechanism (not shown) are further provided.

The spacing of the spotting openings (20a) (2 (lb)) is set so that the openings (15) for passing the solution to be measured in the long sheet (1) can face each other at the same time.

Therefore, in the case of this embodiment, the measurement target solution is spotted through the downstream spotting opening (20a), and the calibration standard solution is spotted through the upstream spotting opening (20b). It only needs to be determined in advance, and even when an inexperienced person performs the spotting work, it is possible to eliminate the inconvenience of making a mistake in the spotting location.

To explain in more detail, when simply measuring the concentration based on the solution to be measured, the downstream spotting opening (20a
), and then the motor (2B) can be driven in the same way as in the above embodiment, and by feeding the long sheet (1) by the minimum necessary distance, the diffusion-limiting membrane (16) can be applied. By bringing the electrode into close contact with the concentration measuring electrode (22), a constant concentration 1-3 operation can be performed.

In addition, in order to prevent the measurement error of the concentration measuring device from increasing over time by measuring the concentration based on a calibration standard solution with a known concentration before performing the concentration measurement operation based on the iIN constant target solution, As shown in the timing chart in FIG.
b) Spot the calibration standard solution through. Next, the motor (2B) is driven in the same manner as in the above embodiment to rotate the long sheet (1).
), and a diffusion-limiting membrane (1G
) can be brought into close contact with the concentration measuring electrode (22) to perform a calibration operation. After that, the motor (2
7) to rewind the long sheet (1), and bring the diffusion limiting membrane (IG) on which the solution to be measured is deposited into close contact with the concentration 111J constant electrode (22) to perform a concentration Δ-j constant operation. I can make you do it. And finally the motor again (
26) to feed the long sheet (1), and simultaneously align the opening (15) for passage of the unused solution to be measured with the openings (20a) and (20b) for both point application, thereby performing the next concentration measurement. Prepare for action.

In order to improve the positioning accuracy when rewinding the long sheet (1), as shown in FIG. 9B, an ON signal is output from the sensor (24), and an OFF signal is output from the sensor (25). The motor (27) until the signal is output.
) is rotated forward to rewind the long sheet (1), and then the motor (2) is rotated until the sensor (24) outputs an OFF signal and the sensor (25) outputs an ON signal.
6) in the normal direction to feed the long sheet (1), it is possible to reliably eliminate the decrease in positioning accuracy caused by the size of the position detection hole (13), etc.

As is clear from the above explanation, even if the calibration operation and concentration measurement operation are performed consecutively in this order, there is no need to change the spotting position of the solution to be measured, and spotting errors can be avoided. This can be reliably prevented.

In addition, in this embodiment, the upstream dotting opening (20b
) If the light emitting element (29) is provided directly facing the light emitting element (29), the light can be recognized through the diffusion limiting film (lB) and the opening (15), so that the effect of preventing point W mistakes can be further enhanced.

<Example 3> Fig. 1O is a partially cutaway perspective view showing still another embodiment of the concentration Δ1j fixing device, and Fig. 11 is a longitudinal sectional view, which is different from the embodiment shown in Figs. 7 and 8. The points are long sheets (1
) A storage solution storage tank (21) is located away from the travel path.
are arranged, only the concentration measuring electrode (22) is reciprocated by the motor (2B), the long sheet (1) is reciprocated by the motor (27), and the opening for passing the AI constant target solution. The only difference is that the long sheet (1) on which only (15) is formed is used. Note that (18b) is a cleaning member for wiping off the solution to be measured, etc. (see FIG. 11A).

The electrode drive mechanism (6) for reciprocating the concentration flP + constant electrode (22) by the motor (26) is shown in Fig. 12A-
As shown in C, there is a screw shaft (61) that is rotated forward and backward by a motor (26), a base (62) for supporting the concentration measurement electrode screwed onto the screw shaft (61), and a base (B2). ) has a groove (64) that guides a protrusion (63) that is integrally provided with the protrusion (63), and a restriction part (65) that restricts movement of the protrusion. The groove (64) has a horizontal portion (64a) extending in the horizontal direction.
and vertical parts (84b) (64c) extending upward at both ends of the horizontal part ([14a)]. Therefore, by simply switching the direction of rotation of the screw shaft (61) between the horizontal part (14a) and the vertical part (84b), the protrusion (63) can move along the horizontal part (64a). A state of movement along the vertical portion (84b) can be obtained. Further, the regulating portion (65) has a protrusion (B3
) moves along the horizontal part (84a) and the vertical part (84a).
84 (G5a) as a fulcrum, and can be rotated by a predetermined angle using the axis (G5a) as a fulcrum, and when the dead point is exceeded, the spring (85b) moves the It has a swinging member (85c) that is rotationally biased, is rotatable by a predetermined angle about a shaft (65d) as a fulcrum, and has a spring (65c).
spring (05e) adjusted between the point of application by 5b)
It has a stopper arm (651') that is selectively biased to rotate to either side. A part of the swinging member (85c) is arranged in the horizontal part (64a) or in the vertical part (84) depending on the direction of the rotation biasing force by the spring (85b).
c), and the amount of protrusion is set so that the dead point can be exceeded by being forcibly pushed in by the protrusion (63). Further, the tip of the stopper arm (65r) is arranged in a horizontal portion (
04a) or the end of the vertical portion (84c). Therefore, the protrusion (63) is horizontal [(64a
) and pushing the swinging member (85c),
The stopper arm (85f') is in a state where it can prevent the protrusion (63) from moving backwards, and the protrusion (83) is in the straight position (84c).
By moving and pushing the swinging member (65c), -
A state is reached in which the protrusion (63) can be prevented from moving back after it has been lowered. Note that in order to achieve the same effect, a solenoid plunger or the like may be used instead of the above configuration.

The sheet drive mechanism (7) for reciprocating the long sheet (1) by the motor (27) has a driving gear (7■) on the rotating shaft of the motor (27), as schematically shown in Fig. 13. A switching gear (72) that meshes with the drive gear (71) is supported by a support plate (73) that is rotatable about the rotation axis. Then, support plate (7
The first transmission mechanism (74a) meshes with the gear (72) at the bidirectional rotating position of 3) and transmits the rotational force in the feeding direction to the long sheet (1), and the first transmission mechanism (74a) transmits the rotational force in the rewinding direction. It has a second transmission mechanism (74b). therefore,
The gear (72) rotates in the rotation direction of the driving gear (71) and meshes with the corresponding transmission mechanism, so that the motor (27)
Simply select the rotation direction of the long sheet (1),
Rewinding can be easily controlled.

In addition, (17a) is a supply shaft, and (18a) is a winding shaft.

The operation of the concentration allJ constant device having the above configuration is as follows.

When performing only concentration a-j constant operation based on all constant target solutions, after spotting the target solution to the opening (15) for passing the target solution through the downstream spotting opening (20a), the motor (27) to feed the long sheet (1) a predetermined distance. Next, the motor (26
), the concentration measuring electrode (22) is lowered and separated from the storage solution storage tank (21), rotated horizontally, and then raised to bring it into close contact with the diffusion restriction membrane (16) for concentration measurement. . Thereafter, by driving the motor (26) in the reverse direction, the concentration measuring electrode (22) is brought into contact with the storage solution storage tank (21) in preparation for the next constant operation at a concentration of 71p. Thereafter, by repeating the above series of operations, concentration measurement electrodes can be measured multiple times without mounting or discarding the diffusion limiting membrane. In this case, concentration? In conjunction with the opening operation for J#j determination, the long sheet (1) is rewound by the distance between the openings (15), and in this state, the concentration δ-1 constant operation is performed in the same manner as above, and finally It is preferable to send the long sheath H1) by a distance twice the distance between the openings (15), so that the spotted n1 constant target solution can be wiped off immediately after the concentration measurement operation is completed.

When performing a calibration operation based on the calibration standard solution before performing a concentration measurement operation based on the n1 constant target solution, the concentration is passed through the upstream spotting opening (20b) to the opening (15) for passing the target solution. After spotting a known calibration standard solution and spotting the solution to be measured through the spotting opening (20a) to the opening (15) for passage of the solution to be measured, the motor (27) is driven. Feed the sheet (1) by a predetermined distance. However, in this case, the predetermined distance is the aperture (1
5) Since the distance is about twice the distance between the two, the diffusion-limiting membrane (1B) covers the opening (15) where the calibration standard solution is spotted.
comes into close contact with the concentration measuring electrode (22). Next, by driving the motor (2B), the concentration measuring electrode (22) is lowered and separated from the storage solution storage tank (21), rotated horizontally, and then raised to bring it into close contact with the diffusion restriction membrane (16). Then, the concentration n1 of the calibration standard solution is determined, and a calibration operation is performed. Thereafter, by driving the motor (26) in the opposite direction, the concentration lpj constant electrode 2) is separated from the diffusion limiting membrane (1G), and by driving the motor (27) in the opposite direction, the openings (15) are separated from each other. By rewinding the long sheet (1) by the interval and driving the motor (26) in the forward direction to bring the concentration measuring electrode (22) into close contact with the diffusion limiting membrane (1B), concentration measurement based on the 4Ilj constant target solution is performed. can be done. And finally, the motor (2B
) by driving the concentration measuring electrode (22) in the opposite direction.
The long sheet (
1) by the distance between the openings (15) to prepare for the next concentration measurement operation (see the timing chart shown in FIG. 14A).

Thereafter, by repeating the above series of operations, the calibration operation and concentration measurement operation can be performed multiple times without mounting or discarding the diffusion limiting membrane. In this case, the long sheet (1) is first fed so that a calibration operation based on the calibration standard solution is performed, and then the long sheet (1) is rolled so that a concentration ΔIIJ constant operation is performed based on the solution to be measured. It is preferable to return the long sheet (1) and finally send the long sheet (1) by twice the distance between the openings (15) to wipe off the spotted calibration standard solution and measurement target solution immediately after the operation is completed. Can be done.

In addition, in order to improve the positioning accuracy when rewinding the long sheet (1), as shown in FIG. 14B, the motor (2
7) is reversed to rewind the long sheet (1), and then
It is sufficient to send the long sheet (1) by rotating the motor (27) in the normal direction until an OFF signal is output from the sensor (24).
Deterioration in positioning accuracy caused by the size of the position detection hole (13), etc. can be reliably eliminated.

In this embodiment as well, the upstream dotting opening (20
If a light emitting element (not shown) is provided directly facing b),
Since light can be recognized through the diffusion limiting film (16) and the opening (15), the effect of preventing spotting errors can be further enhanced.

<Example 4> FIG. 15 is a schematic perspective view showing still another example,
The difference from the above embodiment is that there is a pair of dotting openings (20a).
The only difference is that a cover (28) for selectively covering (20b) is provided so as to be horizontally rotatable.

The cover (28) is rotated in conjunction with the lid of the concentration 81j fixing device, and which spotting opening is selected by a measurement mode selection switch (not shown) attached to the concentration n1 fixing device. We are trying to control whether it should be covered or not. However, the concentration measurement electrode (
The cover (28) is automatically detected based on the state of 22) or set by manual operation, and corresponding to the detected state or set state, the cover (28) is It may be made to rotate. A small diameter hole (28a) for spotting the calibration standard solution is formed at a predetermined position on the side that covers the downstream spotting opening (20a).

FIG. 16 is a diagram showing details of the mechanism for rotating the cover (28), in which the first arm (2C
) are rotatably connected via a second arm (2d), and the first
The movement of the tip of the arm (2c) is restricted. A regulating bin (2g) for regulating the movement of the cover (28) is provided at the tip of the first arm (2C). Furthermore, a spring (28b) is provided which urges the cover (28) to rotate toward the regulation bin (2g), and a spring (28b) is provided at a predetermined position of the cover (28) to engage with the regulation bin (2g). A joint (28c) is provided.

Then, place the cover (28) at a predetermined position on the main body of the concentration measuring device.
A repulsion type electromagnet (28d) that attracts and holds the is provided.

Therefore, when performing only the concentration measurement operation, the power supply to the repulsion type electromagnet (28d) is stopped.
The cover (28) continues to be suctioned and held against the spring (28b), and the cover (28) continues to be rotated so as to cover the dotting opening (20b) on the upstream side (16th
(see Figures A and C). As a result, the downstream dotting opening (
The opening (15) for passage of the solution to be measured through only the
) is exposed, it is only necessary to apply the solution to be measured in the exposed opening (15), and then simply perform the feeding operation of the long sheet (1) and the movement operation of the concentration measuring electrode (22). Concentration measurement operation can be performed with .

Furthermore, when performing a calibration operation prior to the concentration measurement operation, power is supplied to the repulsion type electromagnet (28d), so the cover (28) is rotated in the opposite direction by the spring (28b). (See Figure 16B). As a result, the opening ([5) for passage of the measurement target solution is largely exposed through only the upstream spotting opening (20b), and the measurement target solution is passed through the downstream spotting opening (20a) and the small diameter hole (28a). Since the passage opening (15) is slightly exposed,
It is sufficient to spot the solution to be measured in the widely exposed opening (15) and the calibration standard solution in the slightly exposed opening (15), and then feed the long sheet (1). The calibration operation and the concentration measurement operation can be sequentially performed by simply repeating the movement operation of the concentration measurement electrode (22) twice.

As is clear from the above explanation, in this example,
Since only the opening (15) that allows spotting of the solution to be measured is largely exposed, the occurrence of spotting mistakes can be effectively prevented.

The mechanism for rotating the cover (28) is as follows:
The structure is not limited to the above structure, and it is possible to rotate it independently using a solenoid or the like, for example.

<Example 5> Fig. 17 is a schematic perspective view showing still another example of the concentration measuring device, and Fig. 18 is a longitudinal cross-sectional view. The only difference is that a disc-shaped shea) (Ia) is accommodated instead of.

More specifically, a space is formed in a predetermined position of the casing (2a) to accommodate a disc-shaped sheet (1a) housed in a thin large-sized cartridge (11a). An opening (20) for spotting is formed at a predetermined position on the running path of the disc-shaped sheet (1a), and
A preservation liquid storage tank (21) is arranged at a position downstream of the opening (20) and slightly away from a predetermined position. Then, the concentration side constant voltage 1M (22) is attached to the disk-shaped sheet (1a).
1lPJ fixed position (21b) (opening (20
) and the storage solution storage tank (
An electrode reciprocating mechanism (similar to the mechanism shown in FIGS. 11 to 13, and detailed explanation thereof will be omitted) is arranged for reciprocating the electrode between storage positions in close contact with the electrode 21). Note that (23) is a rotation shaft for rotating the disc-shaped seat (1a), and is driven by a motor (not shown).

In addition, the disk-shaped sheet (1a) has a plurality of openings (15) for passage of the solution to be measured at predetermined positions near the outer periphery, and a diffusion-limiting film (15) so as to cover each opening (I5). 16).

Therefore, after spotting all the target solutions into the opening (15) for passing the measurement target solution exposed through the spotting opening (20), the disc-shaped sheet (1a) is rotated by a predetermined angle. Then, by moving the concentration API constant electrode 2) and bringing it into close contact with the diffusion limiting film (16), a concentration, IFJ constant operation can be performed. After that, concentration? The JPJ constant electrode (22) is moved in the opposite direction and brought into close contact with the storage solution storage tank (21) in preparation for the next concentration measurement.

That is, also in the case of this embodiment, the number of times the diffusion restriction membrane (16) is attached or discarded can be significantly reduced, and the risk of disease infection can be significantly reduced.

Further, in this embodiment, the opening (20) for dotting is formed in only one place, but similarly to the above embodiment, the opening for dotting dotting is formed in two places, and the opening (20) for dotting is formed in two places. It is also possible to provide a cover that selectively covers the opening for dotting.

In the embodiment of the present invention, a long sheet (1) in which holes (13) (13a) (14) are formed is used to detect the end of the sheet. In addition, since the distance between the opening (15) and the large-diameter opening (15a) is predetermined, the positioning hole can be omitted and the operating amount of the motor can be reduced. By controlling it, it is possible to position the opening (15) and the large-diameter opening (15a), and it is also possible to form a hole for tip detection.

<Effects of the Invention> As described above, the first invention not only eliminates the need for attaching and detaching the diffusion limiting membranes while performing the 4p1 constant equal to the number of diffusion limiting membranes provided in the diffusion limiting membrane holder. , 4p1 constantThe point where the target solution is applied and the point where the concentration 1 constant is performed by the concentration measuring electrode are far apart, so the 71Pj of the body fluid is
It has the unique effect of eliminating the risk of disease infection even when the virus is infected.

The second invention has the unique effect of being able to move the thin sheet in one direction without touching the thin sheet.

The third invention reverses the order in which concentration measurements are performed based on each solution by depositing solutions in advance in two openings of a thin plate sheet and then performing backward and forward movements in this order. It has the unique effect of being able to

The fourth invention allows the solution spotting operation to be easily performed through the solution spotting part, and after the solution spotting, the diffusion limiting membrane holder is simply moved by the same distance as the rjR port for passing the target solution of 71111. It has the unique effect of being able to perform the concentration measurement operation as it is, and also being able to perform the next solution spotting operation.

The fifth invention is such that a solution spotting section for spotting a solution with an unknown concentration and a solution spotting section for spotting a solution with a known concentration can be separated in advance, and a solution spotting section for spotting a solution with a known concentration can be separated in advance. The unique effect is that it is possible to reliably perform a configuration operation based on the concentration DI and a concentration DI constant operation based on a solution whose concentration is unknown.

The sixth invention is capable of selecting between a state in which only a solution of unknown concentration can be applied and a state in which a solution with a known concentration and a solution with an unknown concentration can be applied, depending on the state of the cover. When the latter condition is selected, the unique effect is that both solutions are applied at the same time and the calibration operation and concentration measurement operation can be performed in this order by moving the thin sheet back and forth. play.

In the seventh invention, the solution can be spotted into the opening for permeation of each substance to be measured through both solution spotting parts, and the solution spotting part to which the solution to be measured whose concentration is known is to be spotted and the concentration are different from each other. This has the unique effect of being able to clearly identify the solution black stone portion into which the unknown 71p1 constant target solution is to be dropped.

[Brief explanation of the drawing]

Fig. 1 is a partially cutaway perspective view showing an embodiment of the concentration Apl determining device of the present invention, Fig. 2 is a vertical sectional view, Fig. 3 is a perspective view of a diffusion limiting membrane holder, and Fig. 4 is a diffusion limiting device. A partially cutaway perspective view showing the membrane holder housed in the cartridge, Figure 5 is a diagram showing the electrode lifting and lowering mechanism, Figure 6 is a timing chart showing an example of the operation of each part, and Figure 7 is a diagram of the concentration measuring device. A partially cutaway perspective view showing another example, Figure 8 is a vertical cross-sectional view, Figure 9 is a measurement of the concentration of the standard solution for calibration and the concentration Δ of the test solution.
10 is a partially cutaway perspective view showing still another embodiment of the concentration 71111 constant device; FIG. 11 is a vertical sectional view; FIG. 12 13 is a schematic diagram showing the long sheet reciprocating mechanism, and FIG. 14 is a diagram showing the electrode reciprocating mechanism.
The figure is a timing chart when measuring the concentration of a standard solution for calibration and the concentration of a test solution in this order. FIG. 15 is a schematic perspective view showing still another embodiment.
17 is a schematic perspective view showing still another embodiment of the concentration measuring device, and FIG. 18 is a longitudinal cross-sectional view. (31)...Rotational force transmission mechanism agent

Claims (1)

[Claims] 1. Openings (15) for passage of the solution to be measured are formed at predetermined intervals in the thin sheet (1) (1a), and
Concentration measuring electrodes (22 ) is provided, and the opening (15
) and the measuring part (21a) for bringing the concentration measuring electrode (22) into contact with the diffusion limiting membrane (16) at a predetermined distance in the sheet moving direction. A concentration measuring device characterized in that: 2. The concentration measuring device according to claim 1, which is provided with a sheet drive mechanism (31) that moves the thin sheet (1) (1a) only in one direction. 3. The concentration measuring device according to claim 1, further comprising a sheet drive mechanism (7) for reciprocating the thin sheet (1) (1a) in a predetermined direction. 4. The solution spotting parts (20) (20a) (20b) have large diameter openings that are larger than the opening (15) for passage of the solution to be measured, and the solution spotting parts (20) (20a) and the measuring part (21) are separated by the distance between the openings (15) for passage of the solution to be measured. 5. The solution spotting portions (20a) and (20b) are provided at two locations separated by a predetermined distance, and a cover (28) is provided to selectively cover either of the solution spotting portions. A concentration measuring device according to any one of claims 1 to 4. 6. A small-diameter opening (28a) is formed at a predetermined position of the cover (28) to directly face the opening (15) for passage of the solution to be measured exposed through one of the large-diameter openings. The concentration measuring device according to item 5. 7. The solution spotting portions (20a) and (20b) are provided at two locations separated by a distance corresponding to the distance between the openings (15) for passage of the solution to be measured, and one solution spotting portion is provided at two locations. The concentration measurement according to any one of claims 1 to 4 above, wherein the light emitting element (29) is provided below the thin sheet (1) (1a) so as to directly face the part (20b). Device.