JPH02109967A - Rotary stationary bed-type bioreactor - Google Patents
Rotary stationary bed-type bioreactorInfo
- Publication number
- JPH02109967A JPH02109967A JP26291388A JP26291388A JPH02109967A JP H02109967 A JPH02109967 A JP H02109967A JP 26291388 A JP26291388 A JP 26291388A JP 26291388 A JP26291388 A JP 26291388A JP H02109967 A JPH02109967 A JP H02109967A
- Authority
- JP
- Japan
- Prior art keywords
- tank
- culture medium
- biocatalyst
- bioreactor
- mesh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000001963 growth medium Substances 0.000 claims abstract description 32
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
- 108090000790 Enzymes Proteins 0.000 claims abstract description 26
- 239000011942 biocatalyst Substances 0.000 claims abstract description 25
- 230000002503 metabolic effect Effects 0.000 abstract description 3
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 24
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 21
- 229910052760 oxygen Inorganic materials 0.000 description 21
- 239000001301 oxygen Substances 0.000 description 21
- 238000011084 recovery Methods 0.000 description 12
- 239000000969 carrier Substances 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000003638 chemical reducing agent Substances 0.000 description 4
- 239000012510 hollow fiber Substances 0.000 description 4
- 230000003100 immobilizing effect Effects 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000005871 repellent Substances 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000011494 foam glass Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、発酵槽又は培養ｔｆｆ等のバイオリアクタ、
特に回転式の固定層型バイオリアクタに関する。Detailed Description of the Invention [Industrial Application Field] The present invention relates to a bioreactor such as a fermenter or a culture TFF,
In particular, it relates to a rotating fixed bed bioreactor.
発酵又は動植物細胞の培養を行なうバイオリアクタには
、細胞、酵素、酵母、微生物等（以下これらを総称して
生体触媒という）を固定化した担体を浮遊状態に保った
流動層型と、生体触媒を固定化した担体を塔またはカラ
ムに充填した固定層型とがある。さらに、固定層型バイ
オリアクタは、ホローファイバ等のいわゆる中空糸を担
体として用いた中空糸型と、泡ガラス等の粒状担体、セ
ラミックス多孔体等の三次元網目状担体、ハニカム状担
体あるいは多層平板担体を用いた充填層型とに識別され
る。充填層型バイオリアクタの一例として第５図に示さ
れるように、円筒形のタンク内に生体触媒を固定化した
粒状担体を充填し、タンク下端から上方へ培地を供給す
るバイオリアクタがある。この形式のバイオリアクタで
は培地の流れは押出し流れに近く、また、充填層は培地
の流れている移動相と流れのまったくない固定ト■（充
填物の相）から成り立っている。Bioreactors for fermentation or culturing of animal and plant cells include a fluidized bed type in which a carrier on which cells, enzymes, yeast, microorganisms, etc. (hereinafter collectively referred to as biocatalysts) are immobilized is maintained in a suspended state; There is a fixed bed type in which a tower or column is filled with a carrier on which is immobilized. Furthermore, fixed bed bioreactors are of the hollow fiber type using so-called hollow fibers such as hollow fibers as carriers, granular carriers such as foam glass, three-dimensional network carriers such as porous ceramics, honeycomb carriers, or multilayer flat plates. It is classified as a packed bed type using a carrier. As shown in FIG. 5, an example of a packed bed bioreactor is a bioreactor in which a cylindrical tank is filled with a granular carrier on which a biocatalyst is immobilized, and a culture medium is supplied upward from the bottom of the tank. In this type of bioreactor, the flow of the medium is close to an extrusion flow, and the packed bed consists of a mobile phase of flowing medium and a stationary phase (filling phase) with no flow at all.
しかしなから、上述の従来の固定層型バイオリアクタの
場合、第６図に示されるように生体触媒は担体の上側に
より多く付着し、担体の下側における生体触媒の付着密
度が小さい。また、バイオリアクタの出口に近づくにつ
れて培地中の栄養分濃度、溶存酸素濃度の低下と老廃物
濃度の上昇により細胞増殖が阻害される。このため、バ
イオリアクタによる生産物の生産効率が低いという問題
がある。かかる問題は、第５図に示されるバイオリアク
タを単に水平方向にした横型のバイオリアクタにおいて
も同様に生じる。However, in the case of the above-mentioned conventional fixed-bed bioreactor, as shown in FIG. 6, more biocatalysts adhere to the upper side of the support, and the adhesion density of the biocatalyst to the lower side of the support is small. Further, as the outlet of the bioreactor approaches, cell growth is inhibited due to a decrease in the concentration of nutrients and dissolved oxygen in the medium, and an increase in the concentration of waste products. For this reason, there is a problem that the production efficiency of products by the bioreactor is low. Such a problem similarly occurs in a horizontal bioreactor shown in FIG. 5, which is simply oriented horizontally.
本発明は上記の事情に鑑み創案されたものであり、生体
触媒が固定層の全域にわたって均一かつ高密度に付着さ
れ、固定層としての機能が十分に発揮され生産効率の高
い固定層型バイオリアクタを提供することを目的とする
。The present invention was devised in view of the above circumstances, and provides a fixed bed type bioreactor in which a biocatalyst is adhered uniformly and densely over the entire fixed bed, the function of the fixed bed is fully exhibited, and production efficiency is high. The purpose is to provide
本発明は軸方向を水平にした円筒形のタンク内に生体触
媒を固定化するための固定層を配設し、前記タンクをそ
の中心軸を中心として回転するような構成とした。In the present invention, a fixed layer for immobilizing a biocatalyst is disposed in a cylindrical tank whose axis is horizontal, and the tank is configured to rotate around its central axis.
本発明のバイオリアクタはタンクの中心軸を中心として
回転し、バイオリアクタ内に培地とともに流入した生体
触媒は、タンク内の固定層の表面積全域にわたって接触
して均一かつ高密度に付着し、固定層としての機能が十
分に発揮されてバイオリアクタによる生産物の生産効率
が著しく高められる。The bioreactor of the present invention rotates around the central axis of the tank, and the biocatalyst that has flowed into the bioreactor together with the medium contacts the entire surface area of the fixed layer in the tank and adheres uniformly and densely to the fixed layer. The function of bioreactor is fully demonstrated, and the production efficiency of products by the bioreactor is significantly increased.
以下、図面を参照して本発明の実施例について説明する
。Embodiments of the present invention will be described below with reference to the drawings.
第１図は固定層型のうち充填層型のバイオリアクタにお
ける本発明の一実施を示す図である。第１図において、
バイオリアクタ１は円筒形のタンク２を軸方向を水平に
して両端部をそれぞれ外側円形のフランジシール部材４
，６で閉塞したものである。フランジシール６のフラン
ジ部７の外周には歯形が形成され、フランジ部７自体が
回転用歯車７ａ（平歯車）として作用し、この回転用歯
車７ａは動力伝達部材１．５．１６を介して駆動減速機
１２により駆動される駆動歯車１４と歯合されている。FIG. 1 is a diagram showing one implementation of the present invention in a packed bed type bioreactor among fixed bed types. In Figure 1,
A bioreactor 1 has a cylindrical tank 2 with its axial direction horizontal and both ends fitted with outer circular flange seal members 4.
, 6. A tooth profile is formed on the outer periphery of the flange portion 7 of the flange seal 6, and the flange portion 7 itself acts as a rotating gear 7a (spur gear). It is meshed with a drive gear 14 driven by a drive reducer 12 .
また、フランジシール部材４，６のタンク閉塞側と反対
側の中心部には軸部４ａ、６ａがそれぞれ突設され、こ
の軸部４ａ、６ａはそれぞれ軸受部８．１０により軸支
されている。したがって、駆動減速機により駆動歯車１
４を回転することにより回転用歯車７ａがタンク２の中
心軸を中心に回転し、バイオリアクタ１が回転される。In addition, shaft portions 4a and 6a are respectively protruded from the center portions of the flange seal members 4 and 6 on the side opposite to the tank closing side, and these shaft portions 4a and 6a are each supported pivotally by a bearing portion 8.10. . Therefore, the drive gear 1 is driven by the drive reducer.
4 rotates the rotation gear 7a around the central axis of the tank 2, and the bioreactor 1 is rotated.
第２図は第１図に示されているバイオリアクタ１の■−
■線断面図であり、第３図は同じ＜ｍ−■線断面図であ
る。第２図、第３図においてタンク２の内側には円筒形
のメツシュ２２がタンク２と同心となるように配設され
、メツシュ２２の内側には軸方向を同一とした複数の酸
素補給用バイブ２４、培地回収用バイブ２６が配設され
ている。Figure 2 shows the bioreactor 1 shown in Figure 1.
This is a cross-sectional view taken along the line (2), and FIG. 3 is a cross-sectional view taken along the same <m--2 line. In FIGS. 2 and 3, a cylindrical mesh 22 is disposed inside the tank 2 so as to be concentric with the tank 2, and inside the mesh 22 are a plurality of oxygen supplementation vibes arranged in the same axial direction. 24, a medium recovery vibrator 26 is provided.
また、メツシュ２２の内側には生体触媒を固定化するた
めの担体が充填されている。さらに、メツシュ２２の外
側のタンク２との間隙部分には培地供給用ノズル２８が
複数配設されている。Further, the inside of the mesh 22 is filled with a carrier for immobilizing the biocatalyst. Furthermore, a plurality of culture medium supply nozzles 28 are arranged in the gap between the mesh 22 and the tank 2 on the outside.
タンク２はガラス、金属等通常のバイオリアクタ用の材
質からなっていてよく、タンク２の直径および長さはバ
イオリアクタの規模に応じて適宜決定することができる
。The tank 2 may be made of a material such as glass or metal that is commonly used for bioreactors, and the diameter and length of the tank 2 can be determined as appropriate depending on the scale of the bioreactor.
タンク２の両端部を閉塞するフランジシール部材４の内
部には、軸部４ａの内部を通って培地供給用ノズル２８
に連通ずるように培地供給用流路４０が形成されている
。また、フランジシール部材６の内部には、軸部６ａの
内部を通って酸素補給用バイブ２４に酸素を送るための
酸素供給用流路４２と、培地回収用バイブ２６により回
収された培地、生産物、老廃物等を次工程へ送るための
培地回収用流路４４とが形成されている。Inside the flange seal member 4 that closes both ends of the tank 2, a culture medium supply nozzle 28 is inserted through the inside of the shaft portion 4a.
A medium supply channel 40 is formed so as to communicate with the medium. Further, inside the flange seal member 6, there is an oxygen supply flow path 42 for sending oxygen to the oxygen supplying vibe 24 through the inside of the shaft portion 6a, and a culture medium collected by the culture medium recovery vibe 26, A culture medium recovery flow path 44 is formed for sending materials, wastes, etc. to the next process.
第２図に示されるように、フランジシール部４の軸部４
ａは、ロータリシール３０を介して軸受部８に液密状に
軸支されている。この軸受部８には培地供給用流路４０
と連通するように培地供給管９がシール部材３１を介し
て液密状に接続されている。また、フランジシール部６
の軸部６ａは、ロータリシール３２ａを介して軸受部１
０に気密状に軸支されている。そして、軸受部１０には
、酸素供給用流路５２および培地回収用流路５４が形成
され、それぞれ軸部６ａ内の酸素供給用流路４２および
培地回収用流路４４と連通ずるように培地回収管１１が
ロータリシール３２ｂおよびシール部材３３を介してそ
れぞれ気密状、液密状に接続されている。そして、前述
したように、駆動減速機１２によって駆動歯車１４が回
転すると、タンク２、フランジシール部材４（軸部４ａ
）、フランジシール部材６（軸部６ａ）が一体的に回転
する。As shown in FIG. 2, the shaft portion 4 of the flange seal portion 4
a is rotatably supported by a bearing portion 8 via a rotary seal 30 in a liquid-tight manner. This bearing part 8 has a medium supply channel 40.
A culture medium supply pipe 9 is fluid-tightly connected via a seal member 31 so as to communicate with the medium supply pipe 9 . In addition, the flange seal portion 6
The shaft portion 6a is connected to the bearing portion 1 via the rotary seal 32a.
0 in an airtight manner. An oxygen supply channel 52 and a culture medium recovery channel 54 are formed in the bearing portion 10, and are in communication with the oxygen supply channel 42 and the culture medium recovery channel 44 in the shaft portion 6a, respectively. The recovery pipe 11 is connected in an air-tight manner and a liquid-tight manner through a rotary seal 32b and a seal member 33, respectively. As described above, when the drive gear 14 is rotated by the drive reducer 12, the tank 2 and the flange seal member 4 (shaft portion 4a
), the flange seal member 6 (shaft portion 6a) rotates integrally.
メツシュ２２は、例えば同一円周上に等間隔で配設した
支持棒材の外側にワイヤーを一定の間隔でスパイラル状
に巻回して形成したワイヤーメツシュ、あるいはパンチ
ングメタル等の金網状のメツシュ等でよい。このメツシ
ュ２２はタンク２と同心状態で両開口端をフランジシー
ル部材４，６により閉塞されているため、タンク２の内
部はメツシュ２２によって内側と外側に２分割され、メ
ツシュ２２の内側には生体触媒を固定化するための担体
が充填されている。The mesh 22 may be, for example, a wire mesh formed by spirally winding a wire at regular intervals on the outside of a support rod arranged at equal intervals on the same circumference, or a wire mesh made of punched metal, etc. That's fine. Since this mesh 22 is concentric with the tank 2 and both open ends are closed by flange seal members 4 and 6, the inside of the tank 2 is divided into two by the mesh 22 into an inside and an outside, and there is no living body inside the mesh 22. It is filled with a carrier for immobilizing the catalyst.
酸素補給用パイプ２４は生体触媒により消費された培地
中の酸素を補給して培地の溶存酸素濃度を一定に保つも
のであり、酸素供給用流路５２゜４２を通って送られて
きた酸素が酸素補給用バイブ２４の壁面を通過して培地
中に補給される。この場合、動植物細胞のような細胞膜
を持たない生体触媒を酸素の気泡が消滅する際に生じる
剪断力から守るために、酸素補給用バイブ２４は酸素を
発泡しない状態で培地中に供給するものが好ましい。こ
のような酸素補給用バイブ２４として、例えば多孔性焼
結ＳＵＳ支持パイプの周囲をはっ水性を有するテフロン
多孔質膜で覆い密封したもの、あるいは、多孔性焼結Ｓ
ＵＳ支持バイブの外周壁に、はっ水性を有するシリコン
薄膜を形成したもの等がある。The oxygen replenishment pipe 24 replenishes the oxygen consumed in the medium by the biocatalyst to keep the dissolved oxygen concentration of the medium constant. It passes through the wall of the oxygen supplying vibe 24 and is replenished into the culture medium. In this case, in order to protect biocatalysts that do not have cell membranes, such as animal and plant cells, from the shearing force that occurs when oxygen bubbles disappear, the oxygen replenishing vibe 24 supplies oxygen into the culture medium in a non-bubbly state. preferable. Such an oxygen replenishment vibe 24 may be, for example, a porous sintered SUS support pipe covered and sealed with a water-repellent Teflon porous membrane, or a porous sintered SUS support pipe.
There are US support vibes in which a water-repellent silicon thin film is formed on the outer peripheral wall.
このような酸素補給用バイブ２４は第３図に示される例
では、メツシュ２２の内側にメツシュ２２と同心の円周
上に位置するように等間隔に６本配設されている。酸素
補給用バイブの配設本数および配設位置はバイオリアク
タの規模に応じて適宜決定することができる。In the example shown in FIG. 3, six such oxygen supplementing vibes 24 are arranged at equal intervals inside the mesh 22 so as to be located on a circumference concentric with the mesh 22. The number and location of the oxygen supplying vibes can be determined as appropriate depending on the scale of the bioreactor.
培地回収用パイプ２６はバイオリアクタ中で生産された
生産物、使用済培地および老廃物を回収し次工程（精製
工程等）へ送るためのものであり、片側開口の多孔性焼
結ＳＵＳパイプの開口側をフランジシール部材６の培地
回収用流路４４に接続したものである。第３図に示され
る例では、培地回収用パイプ２６はメツシュ２２の内側
のメツシュ２２と同心の円周上に位置するように等間隔
に６本および中心部に１本の計７本配設されている。The medium recovery pipe 26 is for recovering products, used culture medium, and waste products produced in the bioreactor and sending them to the next process (purification process, etc.), and is a porous sintered SUS pipe with an opening on one side. The opening side is connected to the medium recovery channel 44 of the flange seal member 6. In the example shown in FIG. 3, a total of seven culture medium recovery pipes 26 are arranged, six at equal intervals and one in the center so as to be located on the circumference concentric with the mesh 22 inside the mesh 22. has been done.
培地供給用ノズル２８はタンク２の軸方向に新鮮な培地
を均一に供給するためにタンク２とメツシュ２２との間
隙部分に配設されるものである。The culture medium supply nozzle 28 is disposed in the gap between the tank 2 and the mesh 22 in order to uniformly supply fresh culture medium in the axial direction of the tank 2.
第２図に示される例では、注射針状のノズルがフランジ
シール部材４の培地供給用流路４０に連通ずるように配
設されている。通常、培地供給用ノズル２８は同一円周
上に等間隔をなすようにタンク２の軸方向に配設され、
各ノズルの先端までの長さは同一でもよく、あるいは周
期的に変化させたもの（例えば、各ノズルの先端がサイ
ンカーブを形成するもの）であってもよい。培地供給用
ノズル２８の配設本数および配設位置はバイオリアクタ
の規模に応じて適宜決定することができる。In the example shown in FIG. 2, a needle-shaped nozzle is arranged so as to communicate with the culture medium supply channel 40 of the flange seal member 4. In the example shown in FIG. Normally, the culture medium supply nozzles 28 are arranged in the axial direction of the tank 2 so as to be equally spaced on the same circumference.
The length to the tip of each nozzle may be the same, or may be changed periodically (for example, the tip of each nozzle forms a sine curve). The number and position of the medium supply nozzles 28 can be determined as appropriate depending on the scale of the bioreactor.
このような本発明に係るバイオリアクタにおいて、培地
供給管９から供給された培地は、フランジシール部材４
内の培地供給用流路４０を通って培地供給用ノズル２８
からタンク２とメツシュ２２との間隙部分に供給される
。バイオリアクタ１のタンク２内では、供給された培地
はメツシュ２２を通過してメツシュ２２内に充填されて
いる担体間をタンク半径方向内側に向って流れ、培地回
収用パイプ２６によって回収され培地回収用流路４４．
５４を通って次工程へ送られる。そして、本発明ではバ
イオリアクタ１は回転しているため、培地とともにタン
ク内に流入した生体触媒は、担体く固定層）の表面積全
域にわたって接触し、第４図に示されるように担体全体
に均一かつ高密度に付着することになる。また、生体触
媒の付着（固定）後の生体触媒による有用物質の生産段
階においても、バイオリアクタ１が回転しているため、
培地が攪拌されて培地の栄養分濃度、溶存酸素濃度がタ
ンク２内部の全域にわたって均一化され、この均一化さ
れた培地が固定層全域に供給される。したがって、生体
触媒の代謝活性が高くなり生産物の生産効率が向上する
。本発明におけるバイオリアクタの回転速度は、生体触
媒の種類、バイオリアクタの規模等から最適な条件を設
定することができ、例えば動物細胞の場合、１〜１６回
転／回転度が好ましい。In such a bioreactor according to the present invention, the culture medium supplied from the culture medium supply pipe 9 is transferred to the flange seal member 4.
The medium supply nozzle 28 passes through the medium supply flow path 40 in the
It is supplied from the tank 2 to the gap between the mesh 22 and the tank 2. In the tank 2 of the bioreactor 1, the supplied medium passes through the mesh 22, flows between the carriers filled in the mesh 22 in the radial direction of the tank, and is recovered by the medium recovery pipe 26 for medium recovery. Flow path 44.
54 and sent to the next process. In the present invention, since the bioreactor 1 is rotating, the biocatalyst that has flowed into the tank together with the culture medium comes into contact with the entire surface area of the carrier (fixed layer), and is uniformly spread over the entire carrier as shown in FIG. And it will adhere with high density. In addition, since the bioreactor 1 is rotating even during the production stage of useful substances using the biocatalyst after attachment (fixation) of the biocatalyst,
The medium is stirred to equalize the nutrient concentration and dissolved oxygen concentration of the medium over the entire area inside the tank 2, and this homogenized medium is supplied to the entire fixed bed. Therefore, the metabolic activity of the biocatalyst is increased and the production efficiency of the product is improved. The rotational speed of the bioreactor in the present invention can be set to an optimal condition based on the type of biocatalyst, the scale of the bioreactor, etc. For example, in the case of animal cells, 1 to 16 rotations/rotation degree is preferable.
本発明のパイオリ−アクタの構造は、上記のタンク半径
方向に培地が流れる実施例の他、タンク軸方向に沿って
培地が流れる構造のものでもよい。In addition to the above embodiment in which the culture medium flows in the radial direction of the tank, the structure of the pie re-actor of the present invention may be such that the culture medium flows in the axial direction of the tank.
本発明において適用可能な生体触媒は、付着性動物細胞
、酵母および微生物等柱々の生体触媒が挙げられる。Biocatalysts that can be used in the present invention include those based on adherent animal cells, yeast, microorganisms, and the like.
また、生体触媒を固定化する固定層は、生体親和性に優
れた公知の粒状担体、三次元網目状担体、ハニカム状担
体および多層平板担体あるいは中空糸等の種々の担体を
用いることができる。Further, as the fixed layer for immobilizing the biocatalyst, various known carriers having excellent biocompatibility such as granular carriers, three-dimensional network carriers, honeycomb-shaped carriers, multilayer flat carriers, or hollow fibers can be used.
本発明によれば、生体触媒が固定層の表面積全域にわた
って均一かつ高密度に付着され、固定層の機能が十分に
発揮されるとともに、培地中の栄養分濃度、溶存酸素濃
度が均一化されることにより生体触媒の代謝活性が維持
され、バイオリアクタによる生産物の生産効率が著しく
向上する。According to the present invention, the biocatalyst is adhered uniformly and densely over the entire surface area of the fixed layer, the function of the fixed layer is fully exhibited, and the nutrient concentration and dissolved oxygen concentration in the medium are made uniform. This maintains the metabolic activity of the biocatalyst and significantly improves the production efficiency of the bioreactor.
第１図は本発明の一実施例の側面図、第２図は第１図の
■−■線断面図、第３図は第１図の■−■線断面図、第
４図は本発明における担体への生物触媒の付着状態を示
す説明図、第５図は従来の充填層型バイオリアクタを示
す図、第６図は従来のバイオリアクタにおける担体への
生体触媒の付着状態を示す説明図である。
１・・・バイオリアクタ、２・・・タンク、４，６・・
・フランジシール部材、４ａ、６ａ・・・軸部、７・・
・フランジ部、７ａ・・・回転用歯車、８，１０・・・
軸受部、９・・・培地供給管、１１・・・培地回収管、
１２・・・駆動減速機、１４・・・駆動歯車、１５．１
６・・・動力伝達部材、２２・・・メツシュ、２４・・
・酸素補給用バイブ、２６・・・培地回収用パイプ、２
８・・・培地供給用ノズル、３０．３２・・・ロータリ
シール、３１．３３・・・シール部材、４０・・・培地
供給用流路、４２．５２・・・酸素供給用流路、４４．
５４・・・培地回収用流路。FIG. 1 is a side view of an embodiment of the present invention, FIG. 2 is a sectional view taken along the line ■-■ in FIG. 1, FIG. 3 is a sectional view taken along the line ■-■ in FIG. FIG. 5 is a diagram showing a conventional packed bed bioreactor, and FIG. 6 is an explanatory diagram showing a biocatalyst attached to a support in a conventional bioreactor. It is. 1... Bioreactor, 2... Tank, 4, 6...
・Flange seal member, 4a, 6a...Shaft portion, 7...
・Flange part, 7a... Rotating gear, 8, 10...
Bearing part, 9... Culture medium supply pipe, 11... Culture medium collection pipe,
12... Drive reducer, 14... Drive gear, 15.1
6... Power transmission member, 22... Mesh, 24...
・Oxygen supply vibrator, 26... Culture medium collection pipe, 2
8... Culture medium supply nozzle, 30.32... Rotary seal, 31.33... Seal member, 40... Culture medium supply channel, 42.52... Oxygen supply channel, 44 ．．
54...Medium recovery channel.
Claims (1)
固定化するための固定層を配設し、前記タンクをその中
心軸を中心として回転することを特徴とする回転式固定
層型バイオリアクタ。 ２、培地が前記タンク内を半径方向外側から内側に向っ
て流れることを特徴とする請求項１記載の回転式固定層
型バイオリアクタ。 ３、培地が前記タンク内を半径方向内側から外側に向っ
て流れることを特徴とする請求項１記載の回転式固定層
型バイオリアクタ。[Claims] 1. A fixing layer for fixing a biocatalyst is provided in a cylindrical tank with a horizontal axis, and the tank is rotated about its central axis. Rotating fixed bed bioreactor. 2. The rotating fixed bed bioreactor according to claim 1, wherein the culture medium flows in the tank from the outside in the radial direction to the inside. 3. The rotating fixed bed bioreactor according to claim 1, wherein the culture medium flows in the tank from the inside in the radial direction to the outside.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63262913A JPH0687768B2 (en) | 1988-10-20 | 1988-10-20 | Rotary fixed bed bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63262913A JPH0687768B2 (en) | 1988-10-20 | 1988-10-20 | Rotary fixed bed bioreactor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02109967A true JPH02109967A (en) | 1990-04-23 |
| JPH0687768B2 JPH0687768B2 (en) | 1994-11-09 |
Family
ID=17382348
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63262913A Expired - Lifetime JPH0687768B2 (en) | 1988-10-20 | 1988-10-20 | Rotary fixed bed bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0687768B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518693A (en) * | 2020-04-30 | 2020-08-11 | 王家文 | Rotary radial spreading axial multi-sheet adherent cell culture device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6158581A (en) * | 1984-08-31 | 1986-03-25 | Kirin Brewery Co Ltd | Bioreactor |
| JPS6188876A (en) * | 1984-10-09 | 1986-05-07 | Japan Synthetic Rubber Co Ltd | Method and apparatus for culture of plant tissue |
-
1988
- 1988-10-20 JP JP63262913A patent/JPH0687768B2/en not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6158581A (en) * | 1984-08-31 | 1986-03-25 | Kirin Brewery Co Ltd | Bioreactor |
| JPS6188876A (en) * | 1984-10-09 | 1986-05-07 | Japan Synthetic Rubber Co Ltd | Method and apparatus for culture of plant tissue |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111518693A (en) * | 2020-04-30 | 2020-08-11 | 王家文 | Rotary radial spreading axial multi-sheet adherent cell culture device |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0687768B2 (en) | 1994-11-09 |
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