JPH01304824A - Rooting of cultured shoot - Google Patents
Rooting of cultured shootInfo
- Publication number
- JPH01304824A JPH01304824A JP13144988A JP13144988A JPH01304824A JP H01304824 A JPH01304824 A JP H01304824A JP 13144988 A JP13144988 A JP 13144988A JP 13144988 A JP13144988 A JP 13144988A JP H01304824 A JPH01304824 A JP H01304824A
- Authority
- JP
- Japan
- Prior art keywords
- rooting
- cultured
- shoots
- medium
- auxin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 22
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 17
- 229930192334 Auxin Natural products 0.000 claims abstract description 16
- 239000002363 auxin Substances 0.000 claims abstract description 16
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000012882 rooting medium Substances 0.000 claims abstract description 13
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 11
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 9
- 241000196324 Embryophyta Species 0.000 claims description 9
- 238000005273 aeration Methods 0.000 claims description 4
- 230000029553 photosynthesis Effects 0.000 claims description 4
- 238000010672 photosynthesis Methods 0.000 claims description 4
- 235000011449 Rosa Nutrition 0.000 claims description 3
- 239000002609 medium Substances 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 4
- 229930006000 Sucrose Natural products 0.000 abstract description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract description 3
- 239000005720 sucrose Substances 0.000 abstract description 3
- 239000000463 material Substances 0.000 abstract description 2
- 239000007789 gas Substances 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 4
- 241000220317 Rosa Species 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 235000010295 Rosa x kordesii Nutrition 0.000 description 2
- 235000004789 Rosa xanthina Nutrition 0.000 description 2
- 241000109329 Rosa xanthina Species 0.000 description 2
- 230000035425 carbon utilization Effects 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000011490 mineral wool Substances 0.000 description 2
- 239000010451 perlite Substances 0.000 description 2
- 235000019362 perlite Nutrition 0.000 description 2
- 229920000728 polyester Polymers 0.000 description 2
- 238000004382 potting Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 241000967859 Rosa setigera Species 0.000 description 1
- 235000003609 Rosa setigera var setigera Nutrition 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 235000011725 climbing rose Nutrition 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、培養苗条の発根方法に関する。[Detailed description of the invention] [Industrial application field] The present invention relates to a method for rooting cultured shoots.
植物の組織片または培養細胞をＭｉ職培養することによ
って得られた培養苗条の発根は、通常、組織培養に用い
られる基本培地に炭素源及び必要に応じてオーキシンや
サイトカイニン等の植物ホルモンを含有せしめた培地を
用い、培養苗条の培養を従属栄養的、或いは従属栄養と
光独立栄養との混合栄養的条件下で行って発根せしめて
いる。Rooting of cultured shoots obtained by culturing plant tissue pieces or cultured cells is usually done by adding a carbon source and, if necessary, plant hormones such as auxin and cytokinin to the basic medium used for tissue culture. The cultured shoots are cultured under conditions of heterotrophy or mixotrophy of heterotrophy and photoautotrophy using a conditioned medium to cause rooting.
しかしながら、上記従来の発根方法では、発根率が未だ
十分ではなく、ひいては、鉢上げ後の培養菌の活着率や
栽培初期の生育が相対的に低い等の問題があった。However, in the conventional rooting method described above, the rooting rate is still not sufficient, and furthermore, there are problems such as relatively low survival rate of cultured bacteria after potting and relatively low growth at the initial stage of cultivation.
本発明の目的は、上記従来技術の問題点を解消した、新
規な培養苗条の発根方法を提供することにある。An object of the present invention is to provide a novel method for rooting cultured shoots that solves the problems of the prior art described above.
本発明者は、驚くべきことに、培養苗条を、炭素源及び
オーキシンを含まないか、または濃度１％以下の炭素源
と極微量のオーキシンを含む発根培地において、専ら光
独立栄養的に培養するという従来技術と著しく異なる培
養条件を採用することにより、培養苗条の発根率が著し
く増大することを見出し、この新知見に基づいて鋭意研
究を重ねた結果、本発明を完成するに至ったものである
。Surprisingly, the inventors have successfully grown cultured shoots exclusively photoautotrophically in a rooting medium containing no carbon source and auxin or containing a carbon source and trace amounts of auxin at a concentration of less than 1%. They discovered that by adopting culture conditions that are significantly different from those of the conventional technology, the rooting rate of cultured shoots was significantly increased, and as a result of extensive research based on this new knowledge, they were able to complete the present invention. It is something.
したがって、本発明の培養苗条の発根方法の基本的特徴
は、培養苗条を、炭素源及びオーキシンを含まない発根
培地において、所要強度の光照射、所要の炭酸ガス通気
条件下で光合成を行わしめることにより光独立栄養的に
培養しつつ発根させることにあり、或いは、培養苗条を
、微量の炭素源を含み、かつ、苗条の発根時期を揃える
に足る程度の極微量のオーキシンを含む発根培地におい
て、光照射、炭酸ガス供給条件下で光合成を行わしめる
ことにより光独立栄養的に培養しつつ発根させることに
ある。Therefore, the basic feature of the method for rooting cultured shoots of the present invention is that the cultured shoots are photosynthesized in a rooting medium that does not contain a carbon source and auxin, under the conditions of irradiation with light of the required intensity and aeration of carbon dioxide gas. The purpose is to allow the cultured shoots to grow photoautotrophically and to root by soaking the seedlings, or to grow the cultured shoots using a method that contains a trace amount of carbon source and a trace amount of auxin that is sufficient to align the rooting timing of the shoots. The aim is to allow photosynthesis to occur in a rooting medium under light irradiation and carbon dioxide gas supply conditions, thereby culturing photoautotrophically and rooting.
本発明の培養苗条としては、植物の組繊片または培養細
胞から通常の組繊培養によって得られたものを用いるこ
とができ、その−例として、バラ属植物の側芽を組織培
養することによって得られたバラ属植物の培養苗条を挙
げることができる。The cultured shoots of the present invention can be those obtained from plant fiber fragments or cultured cells by conventional tissue culture. Examples include cultured shoots of plants of the genus Rosa.
発根培地としては、水に不溶性の吸水性支持体に培養液
を含浸したものを用いることができる。As the rooting medium, a water-insoluble water-absorbing support impregnated with a culture solution can be used.
前記水に不溶性の吸水性支持体としては、滅菌処理した
パーライト、バーミキュライト、ロックウール、ポリエ
ステル、土、吸水性ポリマ粒子、セラミックウール等の
粒状あるいは繊維状担体を挙げることができる。Examples of the water-insoluble water-absorbing support include granular or fibrous supports such as sterilized perlite, vermiculite, rock wool, polyester, earth, water-absorbing polymer particles, and ceramic wool.
前記培養液としては、ムラシゲとスクーグ、ホワイト、
エッチとニッチ等の基本培養基とし、これに炭素源及び
オーキシンを含まないか、微量の炭素源を含み、かつ、
苗条の発根時期を揃えるに足る程度の極微量のオーキシ
ンを含むものを用いる。The culture solution includes Murashige and Skoog, White,
A basic culture medium such as etch and niche, which does not contain a carbon source and auxin, or contains a trace amount of carbon source, and
Use a material that contains a trace amount of auxin that is sufficient to align the rooting timing of the shoots.
前記苗条の発根時期を揃えるに足る程度の極微量のオー
キシン含量は、具体的には、１Ｏ−９〜ｌＯ−？Ｍの範
囲であり、１０−’Ｍ未満では発根時期がそろわないの
で、また１０−’Ｍを超えると苗条が枯れる−のでいず
れも好ましくない。Specifically, the extremely small amount of auxin content sufficient to align the rooting timing of the shoots is 1O-9 to 1O-? If it is less than 10-'M, the rooting period will not be consistent, and if it exceeds 10-'M, the shoots will wither, so both are not preferred.
本発明の態様によっては＠量の炭素源を加える理由は、
苗条によっては完全に光独立栄養となっていない場合が
あり、その場合でも炭素源と光、炭素ガスによって発根
しうるためである。この場合の微ｌの炭素源の培地にお
ける濃度としては本発明では１％以下である。The reason for adding the amount of carbon source in some embodiments of the present invention is as follows:
This is because some shoots may not be completely photoautotrophic, and even in that case, they can still root using carbon sources, light, and carbon gas. In this case, the concentration of a minute carbon source in the medium is 1% or less in the present invention.
光照射の強度は、光合成を活発に行わせる点から、３
、０００〜２０，０００ルクスの範囲が好ましい。また
、炭酸ガス通気の条件は、光合成の点から、濃度３００
〜１，２００ｐｐｍ、ガス交換率０．１〜３０回／時で
あることが好ましい。The intensity of light irradiation is set at 3 to activate photosynthesis.
,000 to 20,000 lux is preferred. In addition, from the viewpoint of photosynthesis, the conditions for carbon dioxide aeration are a concentration of 300
~1,200 ppm and a gas exchange rate of 0.1 to 30 times/hour are preferred.
本発明の培養苗条の発根方法は、任意の植物に適用する
ことができるが、例えば、バラ属植物の種苗の増殖に極
めて好適である。Although the method for rooting cultured shoots of the present invention can be applied to any plant, it is extremely suitable for propagating seedlings of plants of the genus Rosa, for example.
前記バラ属植物としては、具体的には、ハイブリッドテ
ィーローズ、グランシフローラ、フロリプンダなどの大
、中軸バラやクライミングハイブリッドティーローズ、
ランブラ−などのツルバラおよびマリーアントワネット
、ハイジー、シンブレラなどのミニバラに属する植物を
例示できる。Specifically, the rose genus plants include large and central roses such as hybrid tea roses, grandiflora, and floripunda, and climbing hybrid tea roses;
Examples include climbing roses such as Rambler and plants belonging to mini roses such as Marie Antoinette, Hygiene, and Simbrella.
本発明の発根方法によれば、培養苗条を、炭素源及びオ
ーキシンを含まない発根培地において、光独立栄養的に
培養して発根させることにより、培養苗条の発根率が促
進され、また、発根培地に、極微量のオーキシンを含ま
せることによって、培養苗条の発根時期が揃う作用があ
る。According to the rooting method of the present invention, the rooting rate of the cultured shoots is promoted by photoautotrophically culturing and rooting the cultured shoots in a rooting medium that does not contain a carbon source and auxin, Furthermore, by including a very small amount of auxin in the rooting medium, there is an effect of aligning the rooting timing of the cultured shoots.
実施例１〜３
ミニバラのマリーアントワネットの茎を滅菌して側芽を
採取し、これをショ糖１％およびベンジルアデニン０．
１μＭを含むｐＨ５，７の無菌ムラシゲ・スクーグの液
体培地を含浸させたパーライト、ロックウールもしくは
ポリエステル繊維上に置床し、２５°Ｃで１６時間明朗
（１０，０Ｏ０１ｕｘ） ８時間晴朗の光条件下で培
養した。培養開始後１０日１に、培地中のショ糖が消費
された時点で５００ｐｐｍの炭酸ガスを含む空気をガス
交換率１０回／時の速度で通気した。培養開始後１５日
ロー０．０１μＭのナフクレン酢酸を添加し、合計５週
間培養した後に得られた培養［省を培養容器から取り出
し土に移植した。これらの実施例における発根率および
発根時期に関する結果を表１に示す。Examples 1 to 3 Stems of miniature rose Marie Antoinette were sterilized and lateral buds were collected, which were treated with 1% sucrose and 0.0% benzyladenine.
Place on perlite, rock wool or polyester fibers impregnated with a sterile Murashige-Skoog liquid medium containing 1 μM at pH 5.7 under light conditions of 16 hours at 25°C (10,000 ux) and 8 hours of clear light. Cultured. On the 10th day after the start of the culture, when the sucrose in the medium was consumed, air containing 500 ppm carbon dioxide gas was aerated at a gas exchange rate of 10 times/hour. Fifteen days after the start of culture, 0.01 μM of nafculene acetic acid was added, and after culturing for a total of 5 weeks, the resulting culture was taken out of the culture container and transplanted to soil. Table 1 shows the results regarding the rooting rate and rooting time in these Examples.
比較例１
実施例１において、寒天で固形化した培地を用い、明朗
の照度を１，０ＯＯ１ｕｘとし、また炭酸ガス通気を行
わないこと以外は該実施例と同様にしてミニバラのマリ
ーアントワネソトの側芽を培養した後得られた培養菌を
移植した。本比較例における発根率および発根時期に関
する結果を表１に示す。Comparative Example 1 Side buds of miniature rose Marie Antoine Soto were cultivated in the same manner as in Example 1, except that a medium solidified with agar was used, the illuminance was set to 1,0OO1ux, and carbon dioxide gas was not aerated. After culturing, the resulting cultured bacteria was transplanted. Table 1 shows the results regarding the rooting rate and rooting time in this comparative example.
比較例２
実施例１において、液体培地を用いて酸素を通気し、明
朗の照度を１．５００１ｕｘとし、また炭酸ガス通気を
行わないこと以外は該実施例と同様にしてミニバラのマ
リーアントワネントの側芽を培養した後得られた培養菌
を移植した。本比較例における発根率および発根時期に
関する結果を表１に示す。Comparative Example 2 Miniature rose Marie Antoinent was grown in the same manner as in Example 1, except that a liquid medium was used, oxygen was aerated, the bright illuminance was set to 1.5001 ux, and carbon dioxide gas was not aerated. After culturing the lateral buds, the resulting culture was transplanted. Table 1 shows the results regarding the rooting rate and rooting time in this comparative example.
（木口以下余白）
〔発明の効果〕
本発明の発根方法によれば、培養苗条を、炭素源及びオ
ーホシンを含まない発根培地において、光独立栄養的に
培養して発根させることにより、培養菌の発根率が促進
され、ひいては、鉢上げ後の培養菌の活着率や栽培初期
の生育が相対的に向上する等の顕著な効゛果を奏する。(Margin below the end of the wood) [Effects of the Invention] According to the rooting method of the present invention, by photoautotrophically culturing and rooting cultured shoots in a rooting medium that does not contain a carbon source and orfosine, The rooting rate of the cultured bacteria is promoted, which in turn produces remarkable effects such as a relative improvement in the survival rate of the cultured bacteria after potting and the growth in the early stages of cultivation.
また、発根培地に、極微量のオーキシンを含ませること
によって、培養苗条の発根時期が揃い、事後の作業を効
率化することができる。Furthermore, by including a very small amount of auxin in the rooting medium, the rooting timing of the cultured shoots is aligned, and subsequent work can be made more efficient.
出願人 三井石油化学工業株式会社Applicant: Mitsui Petrochemical Industries, Ltd.
Claims (1)
培地において、所要強度の光照射、所要の炭酸ガス通気
条件下で光合成を行わしめることにより光独立栄養的に
培養しつつ発根させることを特徴とする培養苗条の発根
方法。 ２、光照射の強度が、３，０００〜２０，０００ルクス
の範囲であることを特徴とする請求項１記載の培養苗条
の発根方法。 ３、炭酸ガスの通気の条件が、濃度３００〜１，２００
ｐｐｍ、ガス交換率０．１〜３０回／時であることを特
徴とする請求項１又は請求項３記載の培養苗条の発根方
法。 ４、培養苗条を、濃度１％以下の炭素源を含み、かつ、
苗条の発根時期を揃えるに足る程度の極微量のオーキシ
ンを含む発根培地において、光照射、炭酸ガス供給条件
下で光合成を行わしめることにより光独立栄養的に培養
しつつ発根させることを特徴とする培養苗条の発根方法
。 ５、オーキシンの含量が１０＾−＾９〜１０＾−＾７Ｍ
の範囲であることを特徴とする請求項４記載の培養苗条
の発根方法。 ６、発根培地が、水に不溶性の吸水性支持体に培養液を
含浸させたものからなることを特徴とする請求項１乃至
請求項５記載の培養苗条の発根方法。 ７、植物がバラ属植物であることを特徴とする請求項１
乃至請求項６のいずれかの項記載の培養苗条の発根方法
。[Scope of Claims] 1. Cultured shoots are photoautotrophically cultivated in a rooting medium that does not contain a carbon source or auxin by photosynthesizing under the conditions of irradiation with light of the required intensity and aeration of carbon dioxide gas. A method for rooting cultured shoots, which is characterized by rooting the shoots. 2. The method for rooting cultured shoots according to claim 1, wherein the intensity of light irradiation is in the range of 3,000 to 20,000 lux. 3. The conditions for aeration of carbon dioxide gas is a concentration of 300 to 1,200.
4. The method for rooting cultured shoots according to claim 1 or claim 3, characterized in that the gas exchange rate is 0.1 to 30 times/hour. 4. Cultured shoots containing a carbon source with a concentration of 1% or less, and
In a rooting medium containing a trace amount of auxin that is sufficient to align the rooting timing of shoots, photosynthesis is carried out under light irradiation and carbon dioxide gas supply conditions, allowing rooting to occur while culturing photoautotrophically. Characteristic rooting method of cultured shoots. 5. Auxin content is 10^-^9~10^-^7M
5. The method for rooting cultured shoots according to claim 4, wherein the rooting method is within the range of . 6. The method for rooting cultured shoots according to claims 1 to 5, wherein the rooting medium is made of a water-insoluble water-absorbing support impregnated with a culture solution. 7. Claim 1, wherein the plant is a plant of the genus Rosa.
7. The method for rooting cultured shoots according to any one of claims 6 to 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13144988A JPH01304824A (en) | 1988-05-31 | 1988-05-31 | Rooting of cultured shoot |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13144988A JPH01304824A (en) | 1988-05-31 | 1988-05-31 | Rooting of cultured shoot |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH01304824A true JPH01304824A (en) | 1989-12-08 |
Family
ID=15058220
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13144988A Pending JPH01304824A (en) | 1988-05-31 | 1988-05-31 | Rooting of cultured shoot |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01304824A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05219853A (en) * | 1992-02-13 | 1993-08-31 | Hokko Chem Ind Co Ltd | Method for rooting adventitious embryo of cyclamen |
| JP2016136904A (en) * | 2015-01-28 | 2016-08-04 | 日本製紙株式会社 | Method for evaluating light intensity suitable for rooting |
-
1988
- 1988-05-31 JP JP13144988A patent/JPH01304824A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05219853A (en) * | 1992-02-13 | 1993-08-31 | Hokko Chem Ind Co Ltd | Method for rooting adventitious embryo of cyclamen |
| JP2016136904A (en) * | 2015-01-28 | 2016-08-04 | 日本製紙株式会社 | Method for evaluating light intensity suitable for rooting |
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