JPH0129578Y2 - - Google Patents

Description

[Detailed description of the invention] This invention relates to a device that collects a certain amount of specimen or sample for clinical examination, chemical test, etc. from a container, dilutes it to a certain ratio with a diluent, and dispenses it into a test container. It is something.

Dilution and dispensing devices are widely used in fields such as biochemistry, clinical medicine, chemical industry, and pollution testing. The amount of specimens or samples taken for these inspection tests is becoming smaller and smaller, and the number of inspection test points is also increasing. For this reason, it is required that the dilution and dispensing operation be performed quickly and with high precision. In addition, since a single dilution/dispensing device is required to dilute and dispense samples for various inspection tests, it is possible to easily switch the dispensing amount and dilution rate.
Moreover, it needs to be accurate.

Since the amount of sample to be dispensed in the dilution/dispensing device is very small, a syringe is used to aspirate and discharge the sample and diluent into the device by the movement of its piston, and furthermore, the sample is dispensed by the stroke of the syringe. The amount and dilution rate are adjusted. Therefore,
Conventional dilution and dispensing devices are provided with a stopper for regulating the stroke of the piston.

As described above, it is necessary to dilute and dispense specimens at various dispensing amounts and dilution rates using one diluting and dispensing device. Therefore, stoppers of various lengths are required to regulate the stroke of the piston.
10 to 20 of them are required, and their replacement is complicated and storage is also inconvenient.

The purpose of this invention is to easily change the position of the stopper that regulates the stroke of the syringe piston in order to adjust the dispensing volume and dilution rate, and to quickly and accurately set the desired dispensing volume and dilution rate. The aim is to provide a dilution/dispensing device that allows for

The dilution/dispensing device according to the present invention has two syringes that suction and discharge the sample and diluent into the device by the movement of a piston, and stoppers that respectively regulate the strokes of the pistons. In a dilution/dispensing device that adjusts the dispensing amount and dilution rate of piston rods, a plurality of piston rods are placed between the movement loci of the tip of each piston rod, parallel to the loci and facing each other, and inside each piston rod in a direction perpendicular to the locus. A pair of stopper guides each having a plurality of grooves are provided, each having a stopper whose both ends are fitted and fixed in the opposing grooves of the pair of stopper guides, and the stopper is fixed to a desired stopper guide. This dilution/dispensing device is characterized in that the stroke of the piston is regulated to a desired distance by fitting into the groove, and the amount of sample dispensed and the dilution rate can be adjusted.

Hereinafter, the diluting and dispensing device of the present invention will be explained based on FIG. 1 of the drawings of the embodiment.

A sample syringe 2 and a diluent syringe 4 are attached to the panel 1. Syringe 2 and 4
A piston is inserted into each, and liquid is sucked in when the piston moves downward, and liquid is discharged when the piston moves upward. The respective piston rods are designated 3 and 5, and their tips are designated 3A and 5A. The piston is the piston rod tip 3A
It is moved up and down by a drive mechanism connected to the panel 1 and 5A from the back side of the panel 1. The dotted portions 6 and 7 on the lower side of the piston rod in the drawing are
This is a groove provided for the movement of the connecting part between the piston rod and the drive mechanism. The positions of the grooves 6 and 7 indicate the locus of movement of the piston rod tips 3A and 5A. In the illustrated embodiment, the volumes of the sample and diluent syringes 2 and 4 are 0.05 ml and 0.5 ml, respectively, and the piston stroke is about 60-70 mm. Also, the syringe capacity can be changed.

A branch block 8 having a T-shaped flow path is attached to the upper part of the sample syringe 2, and the inside of the syringe 2 is branched and connected to a sample tube 9 and a connecting tube 13 that is connected to a switching valve 12, which will be described later. . The sample tube 9 is connected to a tube holder 10 having a sample nozzle 11 at its tip.

A switching valve 12 is attached to the upper part of the diluent syringe 4. A connecting tube 13 to the branch block 8 and a diluent tube 14 are connected to the switching valve 12, and the tip of the diluent tube 14 is inserted into a reagent (diluent) bottle 15. The switching valve 12 opens the tube 14 side and closes the tube 13 side when the syringe 4 sucks liquid, and conversely closes the tube 14 side and opens the tube 13 side when discharging the liquid.

The above is no different from the conventional device, and in the conventional device, some kind of stopper is provided to regulate the stroke of the pistons of the syringes 2 and 4, and the sample dispensing amount and dilution rate are adjusted by the position of the stopper. Ta. That is, to operate this device, first, the syringes 2, 4 and each tube 9, 13, 1
4 etc. is filled with diluent and the stopper is placed in an appropriate position. Insert the sample nozzle 11 into the sample in a sample bottle (not shown) and insert the syringe 2,
When the piston 4 is lowered until the tips 3A and 5A of the rod come into contact with the stopper, a predetermined amount of sample is sucked into the sample nozzle 11, and a predetermined amount of diluent is also sucked. At this time, the amount of sample collected is generally small, less than 0.05ml, so
The sample is merely sucked into the sample nozzle 11 or into the sample nozzle 11 and the tube holder 10, and the rest are filled with diluent.
Next, the tip of the sample nozzle 11 is wiped and inserted into a test tube (not shown), and the pistons of the syringes 2 and 4 are raised to their original positions. The sample nozzle 11 first discharges the predetermined amount of sample into the test tube, followed by a predetermined amount of diluent. Since the sample is sucked and discharged in a short time and the sample nozzle 11 is thin, the sample does not diffuse into the diluent remaining in the device and remains, and the sample attached to the inside of the sample nozzle 11 is washed out. It will be done.

This device is characterized by a stopper that restricts the stroke of the pistons of the syringes 2 and 4. That is,
In this device, the piston rod tip 3A
and 5A (corresponding to the positions of grooves 6 and 7), and a pair of stopper guides 20, 20 and 22, 22 are provided parallel to the loci and facing each other. Each stopper guide 20, 20
And inside 22, 22 there are tracks, i.e. grooves 6 and 7.
11 equally spaced grooves 20A and 22A perpendicular to
is provided. This device also includes stoppers 21 and 23 which are fixed by fitting protrusions 21A and 23A at both ends into opposing grooves 20A, 20A and 22A, 22A of the pair of stopper guides 20, 20 and 22, 22. It's ready. The position and spacing of the grooves 20A and 22A of the stopper guide are precisely designed and finished. In particular, the lower surfaces of the grooves 20A, 22A and the projections 21A, 23A are made to join accurately. Grooves 20A, 22A and protrusions 21A,
The stoppers 21 and 23 may be fixed by fitting the stoppers 23A with a spring means or the like so that they can be pulled out with a force exceeding a certain value.

Therefore, by fitting the stoppers 21, 23 into desired grooves of the stopper guides 20, 22, the movement distance of the piston rod tips 3A, 5A, that is, the stroke of the piston, is regulated at the positions of the stoppers 21, 23. The amount of sample dispensed and the dilution rate can be adjusted.

In the illustrated embodiment, the stopper guides 20, 22
There are grooves dividing the piston stroke distance into equal parts, so by fitting a stopper into each groove, the capacity of the syringes 2 and 4 can be reduced by 10,
20, ... 90, 100% of the sample and diluent can be sucked out. In addition, stopper 21, 23
The bosses 21B and 23B at the contact points with the piston rod tips 3A and 5A are made removable by screwing, etc., and when preparing multiple ones with different heights, the amount of sample or diluted liquid to be collected can be further increased. finely,
For example, 5,15...95%, or 4,14,...94%,
It is also possible to set the capacity to a fraction such as .

The dilution/dispensing device of the present invention is constructed as described above,
Since the stopper is operable, the structure of the stopper that restricts the stroke of the syringe piston is simple, the dispensed amount and dilution rate can be quickly changed, and the values of the dispensed amount and dilution rate are accurate. In addition, it is convenient to handle and store since there is no need to provide a large number of stoppers as in the conventional example.

[Brief explanation of drawings]

FIG. 1 is a schematic front view of a panel portion of an embodiment of the dilution/dispensing device of the present invention. 1... Panel, 2, 4... Syringe, 3, 5...
... Piston rod, 6, 7 ... Groove, 8 ... Branch block, 11 ... Sample nozzle, 12 ... Switching valve, 15 ... Reagent (diluent) bottle, 20, 22 ...
...stopper guide, 20A, 22A...groove, 2
1, 23... Stoppa.

Claims (1)

[Scope of utility model registration request] It has two syringes that suction and discharge the sample and diluent into the device by the movement of the piston, and a stopper that regulates the stroke of the piston, and adjusts the amount of sample dispensed and the dilution rate by the position of the stopper. In the dilution/dispensing device,
A pair of stopper guides are provided, sandwiching the locus of movement of the tip of each piston rod, parallel to the locus and facing each other, each having a plurality of grooves perpendicular to the locus inside each stopper guide. A stopper is fixed by fitting both ends into opposing grooves of a stopper guide, and by fitting the stoppers into opposing grooves of a desired stopper guide, the stroke of the piston is made a desired distance. A dilution/dispensing device characterized in that it is configured to regulate the amount of sample to be dispensed and the dilution rate.