JPH01273578A - Filtration method in continuous culture device and apparatus therefor - Google Patents

Abstract

PURPOSE:To prevent the clogging of filter tubes, by closing a group of filter tubes before the clogging of said filter tubes, opening the filter tubes of the other side of filter tube group and peeling the attached material of the filter tubes of the closed group by the liquid flow caused by filtration. CONSTITUTION:A plurality of filter tubes 41, 42... are divided into at least two groups. Before the outer surface of the filter tubes of a group is clogged by the deposition of floating material 17, the flow is switched to the filter tubes of the other group and the floating material attached to the outer surface of the filter tubes of the closed group is peeled by the flow of the liquid in the vessel 1 caused by the filtration with the filter tubes.

Description

[Detailed Description of the Invention] [Object of the Invention] (Field of Industrial Application) The present invention is directed to continuous culture vessels. The present invention relates to a filtration method and device.

(Prior art) Conventionally, culture solution circulation methods or perfusion methods have been used in high-concentration culture vessels and bioreactors, and in these methods, various membrane filtration methods, filtration through porous membrane materials, or centrifugation are used. There is.

However, the former two methods have the problem of clogging of the furnace material, and the latter method involves mechanical complexity, sanitation problems, and energy cost problems.

Therefore, as an alternative to these methods, there is a filtration method called the cross-flow one-type method shown in FIG. 5, which uses furnace material, has less clogging, and can be operated continuously for a long period of time.

In this cross-flow one type, the liquid to be filtered is forced to circulate inside a cylindrical furnace material a from one end to the other end by the discharge pressure of a pump b, and while it passes through the inside of the door material a, it is applied to the outer peripheral surface of the furnace material a. It has a configuration that allows it to pass through and filter it.

(Problem to be Solved by the Invention) However, even with the above-mentioned cross-flow one-way system, the furnace material a
Because floating objects inevitably adhere to the inner circumferential surface of the
There has been a problem in that the pressure loss changes over time, making it extremely difficult to set optimal operating conditions and determine the filtration area due to this pressure loss.

In view of this, it is an object of the present invention to provide a method and device for filtration in a continuous culture vessel that prevents clogging of the door material and enables continuous operation over a long period of time, even though it is a filtration method that uses furnace material. This was done as a.

[Structure of the invention]

(Means for Solving the Problems) In order to solve the problems of the above-mentioned prior art, the present invention provides the following:
At least two filter tubes are suspended and supported in the container, and the outer ends of the filter tubes and the lower part of the container are communicated with each other through a circulation flow path outside the container, so that the liquid in the container is drained from the outer surface of the filter tube. A device that prevents suspended matter from permeating through the outer surface of the filter cylinder while forcing at least part or all of the liquid to pass through the inside, and one or more of the plurality of filter cylinders are included in each group. The filter cylinders of each group are divided into at least two groups, and the outlet sides of the filter cylinders of each group are opened and closed alternately, and the filter cylinders of one group are closed before a pressure loss occurs that causes clogging of the filter cylinders of that group. The filter cylinders of the other group are then opened, and the floating matter adhering to the outer surface of the filter cylinders of the closed group is peeled off by the flow of liquid in the container that occurs during filtration by the filter cylinders of this group. A filtration method in a continuous culture vessel characterized by a container, a plurality of filtration cylinders suspended and supported within the container from the top plate of the container, and an outflow pipe connected to the outlet side of these filtration cylinders, A circulation channel provided outside the container and connecting the respective outflow pipes to a lower part of the container, and an on-off valve provided in the outflow pipe, and one or more of the plurality of filter cylinders are connected to one another. To provide a filtration device for a continuous incubator, characterized in that the on-off valves can be opened and closed alternately for each group.

(Function) When one on-off valve is opened and the other on-off valve is closed to circulate the liquid, this liquid flow causes the liquid in the container to flow out of the filtration cylinder of the group on the open on-off valve side. It permeates from the surface into the inside of the filter cylinder, thereby performing filtration. When the on-off valve on the other side is opened before the deposits attached to the outer surface of the filter tube reach the entire outer surface, and the open on-off valve is closed, the liquid is transferred through the open filter tube. After filtration, the deposits adhering to the outer surface of the filter cylinder on the side where the operation was stopped gradually move to the outer surface of the filter cylinder on the side where the operation was started, and peeling occurs. By operating the filter cylinders of each group alternately in this manner, continuous operation is possible while preventing clogging of the filter cylinders.

(Example) The present invention will be described below with reference to embodiments shown in the drawings.

In FIG. 1, reference numeral 1 denotes a container such as an incubator or a reactor, which is generally used as a suspension culture or a complete mixing tank type reactor, and may also use an attached carrier such as a microcarrier.

A plurality of (two in FIG. 1) filter cylinders 4 are attached to a top plate 3 that is sealed to the upper opening of the container 1 by a fixture 2.
0.4□ penetrates the top plate 3 and is suspended and supported. Film, sintered metal, or ceramic is used as the material for the filter tubes 4□, 42, and the material is selected depending on the type of suspended matter.

For example, the average pore size of common bacteria is 0. 3-1.0
μ, 0.8 to 3 μ for yeast E. coli, 15 for animal cells
0 to 500 μ, and approximately 0.5 to 3”/ for plant cells. Also, the flow rate per filtration area is 0.1 to 0.5 μ/−1−(0.6 to 3 μ/
Goh. 7□. ) is appropriate.

These filter cylinders4. ．． 42 were divided into two groups,
Outflow pipes 50, 5° are connected to the outer ends of each of the filter cylinders 4□, 4□, and these outflow pipes 5□, 5° merge and are connected to the circulation flow path 6. Opening/closing valves 70, 7□, which can be opened and closed manually or automatically, are inserted in 52.

In the illustrated embodiment, the minimum unit has two filter cylinders 4□ and 4°, so there is one filter cylinder in one group, but four filter cylinders are used as shown schematically in Fig. 2. If the filter cylinders 40, 4□, 43.44 are provided, the two filter cylinders 44, 43 are grouped as - group, and the other filter cylinders 42, 44
As one group, these same group of filter cylinders 4□,
43 or 4 4 outflow pipe 50, 53, 5°, 542
``4'' are merged on the way, and after this merge, the on-off valve 7.

72 may be provided for one group.

A pump 8 and an aeration device 9 as a circulation drive source are connected in the middle of the circulation flow path 6, and the end of the circulation flow path 6 is connected to the lower part of the container 1, and the filtered liquid is transferred to the container. 1
It is designed to circulate into the lower part of the tank.

The ventilation device 9 has a large number of ventilation holes 11. ．． 11... are opened, and a vent pipe 13 is installed in a casing 12 that airtightly surrounds the pipe line 10 in the range where the vent holes 1,1,11... exist.
is connected to the vent hole 11.1 by supplying air into the casing 12 through the vent pipe 13.
Air (oxygen) can be supplied into the liquid from 1....

In addition to the pump 8, an injector 14 may be used as the circulation drive source as shown in FIG. 3. That is, an injector 14 is connected in the middle of the circulation channel 6, and a nozzle 15 for feeding pressure fluid (for example, air) is installed upstream of this injector 14.
The liquid can be pumped by pressure fluid ejected from the nozzle 15.

In FIG. 1, reference numeral 16 indicates a filter for the air supply and exhaust system,
17 indicates floating matter.

Next, the operation of the above embodiment will be explained.

Now, open the on-off valve 7, close the on-off valve 7°, and turn on the pump 8.
By driving the , the liquid in the container 1 is circulated into the lower part of the container 1 via the outflow pipe 54, the circulation channel 6, and the aeration device 9 in the middle. Due to this circulating flow, the liquid in the container 1 permeates from the outer surface of the filtration cylinder 41 of the group on the on-off valve 7 side, into the inside of the filtration cylinder 4, and is sucked into the pump 8 side. During this time, suspended matter 17 gradually adheres to the outer surface of the filter cylinder 4.

Before this deposit does not reach the entire outer surface of the filter cylinder 4, open the on-off valve 72 on the other side and close the on-off valve 7, which was open. The water passes through the filter cylinder 4° and is sucked into the pump 8.

At this time, the suspended matter 17 adhering to the outer surface of the filter tube 4□ on the side where the operation was stopped gradually peels off and moves to the outer surface of the filter tube 4゜ on the side where the operation is started, and the filter tube 4□ It flakes off downward from the bottom edge.

Then, the floating matter 17 adheres to the outer surface of this filter cylinder 4° again, and before the adhesion spreads over the entire outer surface, the on-off valve 7° is closed, the above-mentioned on-off valve 7□ is restarted, and the above-mentioned filter cylinder is removed again. Continue operation using 4□.

This prevents the filter cylinder 4□ from clogging due to adhesion of floating matter 17, and allows continuous operation.

The switching timing of the above-mentioned on-off valves 70, 7° varies depending on the viscosity of the liquid, the number of living organisms such as bacteria, their size, stickiness, growth rate, etc., but the switching timing is set by a timer taking these into consideration. This can be done automatically.

FIG. 4 schematically shows an example in which a mechanism is added to completely remove deposits from the filter cylinders 44, 4□ by backwashing, and backwash pipes 18 18 are connected to the outflow pipes 5□, 5□. Connect this backwash pipe 18,.

1'2 18 By opening the backwash valves 19, 192 at any time, pressure fluid is supplied to the inside of the filter cylinder 40, 4°, and the floating matter 17 adhering to the outer surface thereof is forcibly peeled off. be able to.

In the illustrated embodiment, the case where the number of filter cylinders is two in total and the case where there are four in total have been explained, but this number is not limited, and the optimal number is selected depending on the type and properties of the suspended matter 17. Also, the total number may be an odd number instead of an even number. Further, although the illustrated embodiment shows a circulation system completely closed by the circulation channel 6, any system that continuously supplies liquid and serves seven households is within the technical scope of the present invention. Of course.

〔Effect of the invention〕

As explained above, according to the filtration method of the present invention, before the outer surface of the filter cylinders of one group becomes clogged due to adhesion of suspended matter, the filter cylinders of the other group are switched to the filter cylinders of the other group, and during filtration with this filter cylinder, Since floating substances adhering to the outer surface of the filter tube are peeled off, filtration can be continued without interrupting operation, and stable continuous operation can be performed for a long period of time.

Also of the present invention? According to the filtration device, the filtration cylinders of each group can be used alternately by simply switching the opening and closing of the on-off valves of the filtration cylinders of each group, and the opening and closing operations are automatically performed by using solenoid valves as the on-off valves. It has excellent effects such as being easy to perform.

[Brief explanation of the drawing]

FIG. 1 is a configuration diagram showing one embodiment of the present invention, FIG. 2 is a schematic horizontal cross-sectional view of the portion where the filter cylinder exists without showing a modification of the same, and FIG. 3 is an alternative means of the pump in FIG. 1. illustrative diagram,
FIG. 4 is an explanatory diagram of a case where a backwash valve is provided in the embodiment of FIG. 1, and FIG. 5 is an explanatory diagram showing a conventional technique. 1... Container, 3... Top plate, 4□, 4°, 4. ．． 44
...filter cylinder, 5□, 5°, 53. 54... Outflow pipe, 6... Circulation channel, 7. ．． 72...Opening/closing valve, 8
... Pump, 9... Ventilation device. Applicant's agent Mr. Sato Figure 1

Claims (1)

[Claims] 1. At least two filter cylinders are suspended and supported in a container, and the outer ends of the filter cylinders and the lower part of the container are communicated by a circulation channel outside the container. The liquid is permeated from the outer surface of the filter cylinder to the inside, and while at least part or all of the liquid is forcibly circulated, suspended matter is made impermeable on the outer surface of the filter cylinder,
The plurality of filter cylinders are divided into at least two groups, with one or more filter cylinders in each group, and the outlet sides of the filter cylinders in each group are alternately opened and closed, and the filter cylinders in one group are used to prevent clogging. Before a pressure loss occurs that causes a pressure loss, the filtration cylinders of that group are closed and the filtration cylinders of the other group are opened, and the filtration cylinders of this group filter the closed group by the flow of liquid in the container that occurs during filtration. A filtration method in a continuous culture vessel characterized by peeling off suspended matter adhering to the outer surface of a cylinder. 2. A container, a plurality of filtration tubes suspended from the top plate of the container into the container, outflow tubes connected to the outlet side of these filtration tubes, and each of the outflow tubes provided outside the container. It is equipped with a circulation flow path that merges and continues to the lower part of the container, and an on-off valve provided in the outflow pipe, and one or more of the plurality of filter cylinders are set as one group, and the on-off valves are alternately set for each group. A filtration device for a continuous incubator, characterized in that it can be opened and closed at different times.