JPH01240128A - Rotifer feed - Google Patents
Rotifer feedInfo
- Publication number
- JPH01240128A JPH01240128A JP63067962A JP6796288A JPH01240128A JP H01240128 A JPH01240128 A JP H01240128A JP 63067962 A JP63067962 A JP 63067962A JP 6796288 A JP6796288 A JP 6796288A JP H01240128 A JPH01240128 A JP H01240128A
- Authority
- JP
- Japan
- Prior art keywords
- chlorella
- vitamin
- rotifers
- rotifer
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000700141 Rotifera Species 0.000 title claims abstract description 67
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims abstract description 63
- 229940088594 vitamin Drugs 0.000 claims description 21
- 229930003231 vitamin Natural products 0.000 claims description 21
- 235000013343 vitamin Nutrition 0.000 claims description 21
- 239000011782 vitamin Substances 0.000 claims description 21
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 15
- 239000000725 suspension Substances 0.000 claims description 9
- 241000195493 Cryptophyta Species 0.000 claims description 2
- 229930003779 Vitamin B12 Natural products 0.000 abstract description 10
- 235000019163 vitamin B12 Nutrition 0.000 abstract description 10
- 239000011715 vitamin B12 Substances 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 239000013505 freshwater Substances 0.000 abstract description 4
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 abstract 3
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 11
- 229930003451 Vitamin B1 Natural products 0.000 description 10
- 229960003495 thiamine Drugs 0.000 description 10
- 235000010374 vitamin B1 Nutrition 0.000 description 10
- 239000011691 vitamin B1 Substances 0.000 description 10
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 7
- 229930003270 Vitamin B Natural products 0.000 description 6
- 235000019156 vitamin B Nutrition 0.000 description 6
- 239000011720 vitamin B Substances 0.000 description 6
- 241000251468 Actinopterygii Species 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 229930003316 Vitamin D Natural products 0.000 description 2
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 235000019166 vitamin D Nutrition 0.000 description 2
- 239000011710 vitamin D Substances 0.000 description 2
- 150000003710 vitamin D derivatives Chemical class 0.000 description 2
- 229940046008 vitamin d Drugs 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- -1 Ml-potassium Chemical class 0.000 description 1
- 241000224474 Nannochloropsis Species 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- 229930003776 Vitamin B4 Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 150000002823 nitrates Chemical class 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229910021654 trace metal Inorganic materials 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 235000008979 vitamin B4 Nutrition 0.000 description 1
- 239000011579 vitamin B4 Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は魚類の種苗生産に用いられるワムシを効率的に
安定して生産するためのワムシ用餌料に関するものであ
る。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to rotifer feed for efficiently and stably producing rotifers used in the production of fish seedlings.
水産種苗の初期餌料としてワムシは最も重要な餌料であ
り、ナンノクロロプシス、酵母、淡水産濃縮クロレラ等
を用いたワムシの大量培養法の開発により、種苗生産の
飛躍的な増加が可能となった。しかしながら現在でもワ
ムシ培養においては、時としてワムシの増殖不良が起こ
り、そのため種苗生産に支障をきたすことがあるので、
ワムシのより安定な、効率の良い培養法の確立が望まれ
ている。Rotifers are the most important initial feed for aquatic seedlings, and the development of a mass culture method for rotifers using Nannochloropsis, yeast, concentrated freshwater chlorella, etc. has made it possible to dramatically increase seedling production. . However, even today, when cultivating rotifers, poor growth of the rotifers sometimes occurs, which can hinder seed production.
It is desired to establish a more stable and efficient culture method for rotifers.
ワムシ培養の不安定性の一因として、ワムシ培養槽の細
菌相が変化して、ワムシに必要なビタミンＢ１２を細菌
が供給できなくなることが示唆されており、ワムシ安定
培養のために細菌相解明の試みもなされているが、まだ
これをコントロールすることは困難である。またワムシ
培養槽に直接ビタミンＢｌｌを添加する方法も考えられ
るが、ワムシ培養槽１ｏｆｔ当り１〜５ｇと大量のビタ
ミンＤ２２が必要となるうえ、添加したビタミンが他の
細菌等によって分解を受け、ワムシがビタミンを効率的
に利用できず、経済的でないので実用化には至っていな
い。It has been suggested that one of the reasons for the instability of rotifer culture is that the bacterial flora in the rotifer culture tank changes, making it impossible for the bacteria to supply the vitamin B12 necessary for rotifers. Although attempts have been made, it is still difficult to control this. Another option is to add vitamin Bll directly to the rotifer culture tank, but this requires a large amount of vitamin D22, 1 to 5 g per 1of rotifer culture tank, and the added vitamin is degraded by other bacteria, causing the rotifer to grow. However, it has not been put into practical use because vitamins cannot be used efficiently and it is not economical.
本発明の目的は、現在ワムシ餌料として広く使用されて
いる淡水産クロレラに、ワムシに必要なビタミンＢ４２
を高濃度に含有させ、ワムシ餌料として供給することに
より、効率の良い、安定したワムシ生産を可能にする・
ワムシ餌料を提供することである。The purpose of the present invention is to add vitamin B4, which is necessary for rotifers, to freshwater chlorella, which is currently widely used as feed for rotifers.
By containing a high concentration of and feeding it as rotifer feed, efficient and stable production of rotifers is possible.
Providing rotifer food.
本発明は、培地またはクロレラ懸濁液中に添加したビタ
ミンＢ□２を吸収、蓄積したクロレラ藻体を含有するこ
とを特徴とするワムシ餌料である。The present invention is a rotifer feed characterized by containing Chlorella algae that have absorbed and accumulated vitamin B□2 added to a medium or a Chlorella suspension.
クロレラの培養時の培養液、または培養後のクロレラ懸
濁液に、ビタミンＢ１□を添加してクロレラに吸収、蓄
積させることにより、ビタミンＢ□２高含有クロレラを
得ることができる。Chlorella with a high vitamin B□2 content can be obtained by adding vitamin B1□ to the culture solution during chlorella cultivation or to the chlorella suspension after culturing, and allowing the chlorella to absorb and accumulate the vitamin B1□.
クロレラは通常、窒素源として硝酸塩、アンモニウム塩
、尿素などを用い、その他Ｍｌ−カリウム、硫酸マグネ
シウム、硫酸第一鉄などの無機塩、および必要に応じて
グルコース、酢酸、アミノ酸などの有機物を添加した培
養液を用い、明条件または暗条件にて、通気あるいは撹
拌して培養される。これらの培養液を調製する時、適当
量のビタミンＢ１２を培養液中に加えてクロレラを培養
することにより、培養液に添加したビタミンＢ□２は。Chlorella usually uses nitrates, ammonium salts, urea, etc. as nitrogen sources, and other inorganic salts such as Ml-potassium, magnesium sulfate, ferrous sulfate, and organic substances such as glucose, acetic acid, and amino acids are added as necessary. Culture is carried out using a culture solution under light or dark conditions with aeration or stirring. When preparing these culture solutions, vitamin B□2 is added to the culture solution by adding an appropriate amount of vitamin B12 to the culture solution and culturing chlorella.
培養終了時にはほぼすべてがクロレラ中に吸収。At the end of culture, almost all of it is absorbed into chlorella.
蓄積され、非常に効率よくビタミンＢ１□高含有クロレ
ラを製造することができる。このときのクロレラの培養
条件は通常の培養の場合と同様である。chlorella with a high vitamin B1□ content can be produced very efficiently. The culture conditions for Chlorella at this time are the same as those for normal culture.
また−心的なりロレラの培養方法に従ってクロレラを培
養し、培養終了時のクロレラ懸濁液に適当量のビタミン
Ｂ１□を添加して保持すると、約！０時間程度で添加し
たビタミンＢ１２を１００％クロレラに吸収、蓄積させ
ることができる。この方法によれば、培養液を調製する
時にビタミンを添加する方法に比べ短時間で吸収、蓄積
させることができ、滅菌などによるビタミンの分解もな
いことから、さらに効率的なビタミンＢ□２高含有クロ
レラの製造が可能である。In addition, if chlorella is cultured according to the culturing method of Shinnaori Lorella, and an appropriate amount of vitamin B1□ is added to the chlorella suspension at the end of the culture and maintained, approximately Added vitamin B12 can be absorbed and accumulated in chlorella 100% in about 0 hours. According to this method, compared to the method of adding vitamins when preparing the culture solution, the vitamins can be absorbed and accumulated in a shorter time, and there is no decomposition of the vitamins due to sterilization, etc., so it is a more efficient way to increase vitamin B□2. It is possible to produce chlorella containing chlorella.
クロレラのビタミンＤ２２の含有量は、培養液またはク
ロレラ懸濁液中へのビタミンＢ□２の添加量を加減する
ことにより、１〜１１００μｇ／１００ｇの範囲で、任
意の１度のものを製造することができる。The content of vitamin D22 in chlorella can be produced at any one time within the range of 1 to 1100 μg/100g by adjusting the amount of vitamin B□2 added to the culture solution or chlorella suspension. be able to.
その時の培養液またはクロレラ懸濁液中へのビタミンＤ
２２の添加量は、その培養液によって生産されるクロレ
ラの濃度またはクロレラ懸濁液中のクロレラの濃度等に
よって異なる。たとえばクロレラが７ ｇＩＱ生産され
る培養液または同濃度のクロレラｌＩ！ｌｉ濁液では、
ビタミンＢ１□の培養液またはクロレラ懸濁液中への添
加量は０．５〜２００μｇ／１１の範囲で、ビタミンＢ
１□含有クロレラの製造が可能であり１種々の濃度のビ
タミンＢ４２を含有するクロレラを製造することができ
るが、特に０．５〜１００μｇＩＱの範囲が効率的であ
り、２００μｇ／Ωを超える添加では効率は低くなる。Vitamin D into the culture solution or chlorella suspension at that time
The amount of 22 added varies depending on the concentration of chlorella produced by the culture solution, the concentration of chlorella in the chlorella suspension, etc. For example, a culture solution in which 7 gIQ of chlorella is produced or the same concentration of chlorella lI! In li suspension,
The amount of vitamin B1□ added to the culture solution or chlorella suspension is in the range of 0.5 to 200 μg/11.
It is possible to produce chlorella containing 1□.1 It is possible to produce chlorella containing various concentrations of vitamin B42, but the range of 0.5 to 100 μg IQ is particularly efficient, and adding more than 200 μg/Ω Efficiency will be lower.
本発明では、このようにして高濃度にビタミンＢ１□を
吸収、蓄積させたクロレラを製造し、このクロレラをワ
ムシ用餌料として利用する。In the present invention, chlorella that absorbs and accumulates vitamin B1□ at a high concentration is produced in this way, and this chlorella is used as feed for rotifers.
ビタミンＢ１□を最低２０μｇ／１００ｇ、望ましくは
１００〜２００μｇ／１００ｇ程度含有させたクロレラ
をワムシ生産用の餌料として利用することにより、従来
工業的に生産されているワムシ餌料である酵母、淡水産
濃縮クロレラに比べ、ワムシの生育が非常に良くなり、
したがってワムシの効率的な安定生産が可能となる。こ
の場合、ビタミンＢ１□含有クロレラは生あるいは乾燥
物の使用が可能である。By using chlorella containing at least 20 μg/100 g of vitamin B1□, preferably 100 to 200 μg/100 g, as feed for rotifer production, yeast and freshwater concentrates, which are conventional industrially produced rotifer feed, can be used. Compared to chlorella, rotifer growth is much better,
Therefore, efficient and stable production of rotifers becomes possible. In this case, the vitamin B1□-containing chlorella can be used fresh or dried.
またワムシへの投餌方法は毎日、１回または数回適当量
のクロレラを与えるのが一般的であるが、少量の連続的
な投餌または他の餌料と混合して与えることも可能であ
る。In addition, the general method of feeding rotifers is to feed an appropriate amount of chlorella once or several times every day, but it is also possible to feed it continuously in small amounts or by mixing it with other foods. .
従来ワムシのビタミンＢ８２は、ワムシ培養槽内に発生
する細菌により供給されており、このような細菌を抑制
することは難しく、ワムシ培養の不安定の原因の一つで
あったが、本発明の高濃度のビタミンＢ１□を含有する
クロレラをワムシに与えることにより、安定してビタミ
ンＢ１２をワムシに供給することが可能となり、ワムシ
の増殖が良くなるとともに、ワムシ培養の安定化が可能
となる。Conventionally, vitamin B82 for rotifers was supplied by bacteria generated in rotifer culture tanks, and it was difficult to suppress such bacteria, which was one of the causes of instability in rotifer culture. By feeding rotifers with chlorella containing a high concentration of vitamin B1□, it becomes possible to stably supply vitamin B12 to rotifers, which improves rotifer proliferation and stabilizes rotifer culture.
この場合、クロレラは１００％ワムシに取り込まれるた
め、クロレラに含有しているビタミンＤ２２は非常に効
率的にワムシに利用され、ワムシ培養槽１００ｔに対し
クロレラに含有させて添加するビタミンＢＬ２は０．０
２〜０．１ｇと非常に少量で有効である。In this case, since 100% of chlorella is taken up by rotifers, the vitamin D22 contained in chlorella is utilized very efficiently by rotifers, and the amount of vitamin BL2 added to chlorella per 100 tons of rotifer culture tank is 0. 0
It is effective in a very small amount of 2 to 0.1 g.
また魚類に対しても、ビタミンＤ２２は有効であり、欠
乏すると血球数、ヘモグロビン量が低下することが知ら
れている。従ってビタミン８１□含有クロレラでワムシ
を培養することにより、魚類に対しても優れた栄養価を
有するワムシの生産が可能となる。It is also known that vitamin D22 is effective for fish, and that deficiency causes a decrease in blood cell count and hemoglobin content. Therefore, by culturing rotifers with chlorella containing vitamin 81□, it is possible to produce rotifers that have excellent nutritional value even for fish.
実施例１
種々のクロレラ株の培養液にビタミンＢ１２を添加して
培養を行い、クロレラによるビタミンＢ１２の吸収、蓄
積を調べた。培養条件は２５℃、３〜５ｋｌｕｘの条件
で、グルコース２０ｇＩＱ、尿素１．５ｇ＃！、リン酸
−カルシウム１．２ｇ／Ｌ硫酸マグネシウム０．６ｇＩ
Ｑ、鉄ＩＥＤＴ＾１５ｍｇ／ＩＩＩ、微量金属Ａ５溶液
１ ｍＱ／Ｑ、ビタミンＤ２２４０μｇ／１１を含む培
地を使用し、３日間培養した。Example 1 Vitamin B12 was added to the culture solution of various chlorella strains and cultured, and the absorption and accumulation of vitamin B12 by chlorella was investigated. The culture conditions were 25°C, 3-5klux, glucose 20gIQ, urea 1.5g#! , calcium phosphate 1.2g/L magnesium sulfate 0.6gI
A medium containing Q, iron IEDT^15 mg/III, trace metal A5 solution 1 mQ/Q, and vitamin D2240 μg/11 was used and cultured for 3 days.
こうして得られた種々の濃度のビタミンＤ２２を含むク
ロレラをワムシに与えてその餌料価値を調べた。すなわ
ち人工海水ジャマリンＳにクロレラ１５００万細胞／ｍ
Ｑを懸濁し、人工海水で良く洗ったＬ型ワムシを１個体
／ＩＩＩＱ接種して、２５℃、弱光下にて培養を行い、
ワムシ個体数の変化を調べた。Chlorella containing various concentrations of vitamin D22 thus obtained was fed to rotifers and its feed value was investigated. In other words, artificial seawater Jamarin S contains 15 million cells/m of chlorella.
Suspended Q and inoculated one L-type rotifer per IIIQ, which had been thoroughly washed with artificial seawater, and cultured at 25°C under weak light.
We investigated changes in rotifer populations.
結果を第１図に培養日数とワムシ密度のグラフとして示
す。The results are shown in Figure 1 as a graph of culture days and rotifer density.
第１図の結果により、従来の方法で製造したビタミンＤ
２．をほとんど含まないクロレラ（Ａ、Ｂ）で培養した
ワムシは生育が非常に悪く、ビタミンＢユ２含量４．３
μｇ／１００ｇのものもワムシの生育は悪かった。Based on the results shown in Figure 1, vitamin D produced by the conventional method
2. Rotifers cultured on Chlorella (A, B), which contains almost no
Growth of rotifers was also poor with μg/100g.
一方ビタミンＢ１２を２２．４５．１８０μｇ／１００
ｇ以上含むクロレラ（Ｃ，Ｄ、Ｅ）で培養したワムシの
生育は良く、ワムシ餌料として従来のクロレラより非常
に優れていた。On the other hand, vitamin B12 is 22.45.180μg/100
The growth of rotifers cultured with Chlorella (C, D, E) containing more than 1.0 g was good, and it was much better than conventional Chlorella as feed for rotifers.
実施例２
ビタミンＢ １２１００μｇ／１００ｇ含有するクロレ
ラと。Example 2 Chlorella containing 12100μg/100g of vitamin B.
通常の方法で培養したビタミンＤ２２を含まないクロレ
ラでワムシを培養し、その餌料価値を比較した。ワムシ
は１５ｆ１の人工海水中において、２２〜２４℃で通気
条件で培養した。毎日ワムシの個体数を測定し、ワムシ
個体数に見あった量のクロレラを投餌した。ビタミンを
含むクロレラを与えたワムシは４日目、５日目に卵重が
高くなり、ワムシの増殖もはるかに良かった。７日目に
はワムシ密度が６０５／ｄに達したので、ワムシの大部
分を収穫し、一部分で新たに培養を開始した。Rotifers were cultured using conventionally cultured chlorella that does not contain vitamin D22, and their feed value was compared. Rotifers were cultured in 15fl artificial seawater at 22-24°C under aeration conditions. The number of rotifers was measured every day, and chlorella was fed in an amount appropriate to the number of rotifers. The rotifers fed the vitamin-containing chlorella had higher egg weights on the fourth and fifth days, and the rotifers multiplied much better. On the 7th day, the rotifer density reached 605/d, so most of the rotifers were harvested and a new culture was started on a portion.
一方ビタミンＢ工２を含まないクロレラで培養したワム
シは、１１日後のワムシ密度は５５２／ｄに止まった。On the other hand, rotifers cultured on chlorella that does not contain vitamin B2 had a rotifer density of only 552/d after 11 days.
ビタミンを含むクロレラを与えた区では１１日間で３０
．７ｇのクロレラを与えて１５７５万のワムシが生産で
きたのに対し、ビタミンを含まない区では１５．４ｇの
クロレラしか与えることができず、８０４万のワムシ生
産であった。30 in 11 days in the district where chlorella containing vitamins was given.
．． By feeding 7g of chlorella, 15.75 million rotifers were produced, whereas in the area without vitamins, only 15.4g of chlorella could be fed and 8.04 million rotifers were produced.
結果を第２図に培養日数とワムシ密度および卵重のグラ
フとして示す。The results are shown in Figure 2 as a graph of culture days, rotifer density, and egg weight.
本発明によれば、ビタミンＢＬ□を吸収、蓄積したビタ
ミンＢ１□高含有クロレラをワムシ餌料としたため、効
率の良い安定したワムシ生産が可能になり、魚類に対す
る栄養価も高くなる。According to the present invention, since chlorella with a high vitamin B1□ content that has absorbed and accumulated vitamin BL□ is used as rotifer feed, efficient and stable production of rotifers is possible, and the nutritional value for fish is also increased.
第１図および第２図は実施例の結果を示すグラフである
。
代理人 弁理士 柳 原 成
りき蚤日子（
月ト舎日数
手続補正書
特許庁長官 小 川 邦 夫 殿
１、事件の表示
昭和６３年特許願第６７９６２号
２、発明の名称
ワムシ餌料
３、補正をする者
事件との関係 特許出願人
ミナトクシバダイモン
住 所 東京都港区芝大門二丁目４番６号トヨクニ
ηイ
豊国ビル５階
名称 クロレラ工業株式会社
イダ す漏 シュン ダ ロウ
代表者 板波俊太部FIGS. 1 and 2 are graphs showing the results of Examples. Agent: Patent attorney Nariki Fumihiko Yanagihara (Gektosha Days procedure amendment) Director General of the Patent Office Kunio Ogawa 1, Indication of the case Patent Application No. 67962 filed in 1988 2, Title of invention rotifer bait 3, Amendment Relationship with the case involving the person who filed the patent application: Minato Kushibadaimon Address: Toyokuni, 2-4-6 Shiba Daimon, Minato-ku, Tokyo
ηI Toyokuni Building 5th Floor Name Chlorella Industries Co., Ltd. Ida Suro Shun Da Law Representative Itanami Shuntabe
Claims (1)
Ｂ＿１＿２を吸収、蓄積したクロレラ藻体を含有するこ
とを特徴とするワムシ餌料。(1) A rotifer feed characterized by containing Chlorella algae that have absorbed and accumulated vitamin B_1_2 added to a medium or Chlorella suspension.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63067962A JPH0789875B2 (en) | 1988-03-22 | 1988-03-22 | Rotifer feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63067962A JPH0789875B2 (en) | 1988-03-22 | 1988-03-22 | Rotifer feed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01240128A true JPH01240128A (en) | 1989-09-25 |
| JPH0789875B2 JPH0789875B2 (en) | 1995-10-04 |
Family
ID=13360099
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63067962A Expired - Fee Related JPH0789875B2 (en) | 1988-03-22 | 1988-03-22 | Rotifer feed |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0789875B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003055330A1 (en) * | 2001-12-26 | 2003-07-10 | Kyushu Tlo Company, Limited | Feed for culturing animal planktons such as brachionus rotundiformis |
| KR100447940B1 (en) * | 2002-03-21 | 2004-09-10 | 학교법인 한양학원 | Development of profitable prey, green algae Coelastrum sp. |
| WO2010089864A1 (en) * | 2009-02-04 | 2010-08-12 | クロレラ工業株式会社 | Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same |
-
1988
- 1988-03-22 JP JP63067962A patent/JPH0789875B2/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003055330A1 (en) * | 2001-12-26 | 2003-07-10 | Kyushu Tlo Company, Limited | Feed for culturing animal planktons such as brachionus rotundiformis |
| KR100447940B1 (en) * | 2002-03-21 | 2004-09-10 | 학교법인 한양학원 | Development of profitable prey, green algae Coelastrum sp. |
| WO2010089864A1 (en) * | 2009-02-04 | 2010-08-12 | クロレラ工業株式会社 | Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same |
| JP4852662B2 (en) * | 2009-02-04 | 2012-01-11 | クロレラ工業株式会社 | Selenium-containing single-cell microalgae for zooplankton feed and methods for culturing selenium-containing zooplankton using the same |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0789875B2 (en) | 1995-10-04 |
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