JPH01206924A - Method for acclimating plant body regenerated by plant tissue cultivation - Google Patents

Abstract

PURPOSE:To acclimatize a plant body in high yield without damaging individual roots, by acclimatizing a regenerated individual of a plant formed from cultivated cells in the same culture medium without transplantation thereof. CONSTITUTION:An adventitious embryo bud is initially induced by a conventional method and then bedded on a culture medium prepared by adding an MS liquid culture medium to vermiculite, etc., as a support. A vessel is then capped with an aluminum foil. A juvenile plant body, grown to 3-5cm after the passage of 3-4 weeks from the bedding and placed in the vessel is subsequently transferred to an environment at a sufficiently kept humidity. The cap is slowly opened after 2-3 days. The plant body is then acclimatized to natural environment in a state of the opened can for 3-4 days and planted in soil, such as 'AKADAMA TSUCHI' (horticultural soil).

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for acclimating plants regenerated by tissue culture.

In the conventional technology, adventitious buds formed from cultured cells were transplanted into a medium that promotes rooting and allowed to root.

Rooting media usually consist of various inorganic and organic nutrients added with plant hormones and the like, and gelled with agar, agarose, Gelrite, etc. Plants that have sufficiently rooted in the rooting medium are removed from the container, thoroughly washed off with agar, etc., and then planted in an acclimatization medium. At that time, cover the culture medium and plants with plastic, vinyl, plastic wrap, etc. to maintain the temperature. When agar adheres to plants, mold often develops and hinders growth. Gradually remove the covering, such as plastic or vinyl, to adapt to the natural environment. Horticultural soils such as vermiculite, perlite, peat moss, and Akadama soil have been mainly used as acclimatization media.

In addition, in the case of somatic embryos formed from cultured cells, they were transplanted into a regeneration medium to regenerate plants. Regeneration medium usually consists of various inorganic and organic nutrients, plant hormones, etc. added and gelled with agar, agarose, Gelrite, etc. The following method is similar to that used for adventitious bud formation. This is a way to get used to it.

In the conventional technology, as mentioned above, a different medium from the rooting medium and the conditioning medium is used, and when a somatic embryo is formed, a regeneration medium is used prior to these stages.
Mechanical damage to the plant is likely to occur each time it is replanted, and there has been a strong demand for the development of efficient methods, as well as for labor savings.

The present invention has been developed in view of the above-mentioned needs, and by acclimatizing regenerated individuals formed from cultured cells in the same medium without transplanting them, damage to the roots etc. can be avoided. We have found that it is possible to acclimatize efficiently without having to do so.

Therefore, an object of the present invention is to provide a method for efficiently acclimating the regenerated individuals without transplanting them.

The present invention will be explained in detail below.

Means to solve problems In the present invention, somatic embryos are first induced by conventional methods.

Next, the formed somatic embryos are placed in MS medium (Murashige-5) using vermulite or the like as a support.
The cells were placed on a culture medium supplemented with a liquid medium prepared by Koog.

In the present invention, the material used to form the container is one that satisfies heat resistance and pressure resistance, such as glass or polycarbonate. Heat resistance and pressure resistance are determined based on whether steam sterilization or dry heat sterilization is applicable. Since there are no restrictions on size or shape, test tubes, Erlenmeyer flasks, etc. are also suitable for use. Further, aluminum foil, Saran wrap, cotton plug, metal, plastic, etc. can be used for the cap of the container.

Materials preferably used as the support include vermiculite, perlite, peat moss, Akadama soil, etc., and may also include materials used for solution cultivation such as rock wool, rock fiber, and soil sterilized with steam or chemicals. Can be done.

In addition to these materials, materials similar to the above-mentioned materials that can hold a liquid medium, withstand sterilization, and mechanically support a grown plant can be used.

The liquid medium used in the present invention is a mixture of various medium components (inorganic salts, vitamins, amino acids, sugars, etc.) used for plant tissue culture, to which plant hormones, plant growth regulators, etc. are added as necessary. be.

To prevent the growth of mold, fungicides such as Oatscide and Tachigaren can be used together.

In the practice of the present invention, a liquid medium is held on a support, placed in a container, and subjected to steam sterilization or dry heat sterilization for cultivation. Somatic embryos will start regenerating on a medium such as vermiculite, so if you gradually remove the cap or other covering material after 2 to 3 weeks and let them acclimate to the natural environment, you can pot them up after 1 week. Can be done.

In the present invention, unlike conventional techniques, the formed regenerated individuals are not transplanted, so there is no need to wash the gelling agent, and on the other hand, a bactericide can be used, so the growth of mold can be prevented.

EXAMPLES The present invention will be further explained clearly with reference to Examples below.

Example In this example, we will explain the acclimatization of carrots.

Somatic embryos are induced according to a conventional method, and the formed somatic embryos are transferred to Murashi using vermiculite or the like as a support.
The cells were placed on a culture medium supplemented with a liquid medium prescribed by Ge and Skoog. Aluminum foil was used for the cap of the container.

Plantlets are regenerated from somatic embryos for 1 week after being placed on the bed, and 3 to 4
It grew to about 3-5 cm in about a week. The seedlings in this container were transferred to an environment where sufficient humidity was maintained, and after 2 to 3 days, the cap was gradually opened. Thereafter, the plants were allowed to acclimatize to the natural environment for 3 to 4 days with the caps opened, and then planted in soil such as Akadama soil. At that time, there is no need to wash the vermiculite or other soil that has adhered to the roots of the young plants. By employing this method, the acclimatization rate was improved from about 10 to 30% in the conventional method to 80 to 100%. Also, since it only takes about a week from opening the cap to raising the pot, the potting time was much shorter than the conventional method.

According to the present invention, the regenerated individuals can be acclimatized using the same medium without being transplanted, so that the roots of the individuals are not damaged and the acclimatization can be achieved at a high rate.

Claims (1)

[Claims] (1) A method for acclimating a regenerated plant, which is characterized by acclimating a regenerated plant formed from cultured cells by tissue culture in the same medium without transplanting the regenerated individual.