JPH01206259A - Apparatus for washing reaction container - Google Patents

Abstract

PURPOSE:To accurately perform water blank measurement by preventing the adhesion of an air bubble to the wall surface of a reaction container, by inserting a suction nozzle, which is equipped with an air bubble removing member rubbed with the inner wall of the reaction container or moving in the vicinity of said inner wall, in the reaction container. CONSTITUTION:An air bubble removing member 10 is mounted to the leading end of a suction nozzle 8 of a cycle before the injection of blank water and inserted in a reaction container 2 to suck washing water 20. The member 10 is constituted so as to be rubbed with the inner wall of the reaction container 2 in a contact state or moved in the vicinity of said inner wall. Since the air bubbles bonded to the inner wall of the container 2 is collapsed to disappear or pushed downwardly and sucked by the nozzle 8 to be removed. By this method, the adhesion of the air bubble to the wall surface of the container 2 is prevented and water blank measurement can be accurately performed.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a reaction container cleaning device, and particularly to a device for accurately measuring a water blank (100% T) in a reaction container of an automatic analyzer for blood, urine, etc. This invention relates to a reaction vessel cleaning device suitable for.

[Conventional technology]

As described in Japanese Utility Model Application No. 61-70771, conventional devices of this kind clean the reaction vessel by repeatedly injecting and discharging the cleaning water multiple times, and in the final cycle of cleaning, a block for removing the cleaning water is installed. It was supposed to insert a nozzle.

[Problem to be solved by the invention]

Normally, in automatic analyzers, in order to eliminate the drift of the light source of the photometer and the influence of the ambient temperature of the photometer, water plan measurement (filling the reaction container with water that does not absorb light, and measuring the amount of light at that time to the standard value) However, unless the reaction vessel is completely disposable, the reaction vessel after the reaction is sucked up and cleaned, and the reaction liquid inside is washed, and a new sample is analyzed. Reused. Water blank measurements are performed by injecting water into the reaction vessel after washing it. In addition, since the reaction solution contains a considerable amount of surfactant, the top surface of the reaction solution in the reaction container is often bubbling, so if you insert a suction nozzle into the reaction solution and suck up the reaction solution, The bubbles on the upper surface once pass through the transparent surface of the reaction container and reach the bottom of the container, so washing water is first injected. In order to thoroughly clean the reaction vessel, a multi-stage cleaning method is adopted in which the above-mentioned cleaning operation is repeated many times, but when such multi-stage cleaning is performed, the surfactant effect gradually weakens and air bubbles adhere to the transparent surface. It becomes easier to do. If the bubbles on the upper surface of the reaction liquid descend while traveling along the light-transmitting surface each time, the probability that the bubbles will adhere to the light-transmitting surface of the reaction container becomes very high when the surface active effect wears off. When water for water blank measurement is injected into the area where bubbles are attached, the surface activity is weakened.
The bubbles will firmly adhere to the wall of the reaction vessel as air bubbles.

If a water blank is measured with air bubbles still attached, it will give an error to the zero level, which is the standard value of absorbance, and this will also cause a measurement error in the absorbance of the reaction solution measured using that as a standard.

The above-mentioned conventional technology does not take into account the problem of air bubbles adhering during the water blank measurement, and has the problem of generating data porosity.

An object of the present invention is to provide a reaction vessel cleaning device that does not cause bubble adhesion.

[Means to solve the problem]

The above object is achieved by inserting into the reaction vessel a suction nozzle equipped with a bubble removing member that rubs against the inner wall of the reaction vessel before measuring water for water blank measurement or moves near the inner wall. did.

[Function] The air bubble removal member attached to the tip of the cleaning water suction nozzle descends together with the nozzle when it is inserted into the reaction vessel, and descends while scraping or contacting the inner wall of the reaction vessel. Air bubbles attached to the inner wall of the container are pushed down and sucked up by the nozzle, or are crushed and disappear.

〔Example〕

The present invention will be explained in detail below using the embodiments shown in FIGS. 1 and 2.

FIG. 1 is a block diagram showing an embodiment of the reaction vessel cleaning apparatus of the present invention. In FIG. 1, a reaction line 1 is formed by arranging a large number of reaction vessels 2 in a circular shape. As shown by arrow 3, this reaction line 1 is rotated by one rotation plus one container every cycle and then stopped. Further, the absorbance of the reaction liquid 4 in the reaction container 2 is measured by a photometer 5.

When the reaction line 1 is driven in this manner, all the reaction vessels 2 of one reaction line 1 will necessarily cross the optical axis 7 of the light emitted from the light source lamp 6 in every cycle. If the absorbance of the reaction liquid 4 in each reaction vessel 2 is measured at the instant of this crossing, it is possible to measure the reaction liquid 4 in all reaction vessels 2 in every cycle.

On the other hand, the reaction vessel 2 is cleaned by inserting the nozzle of the cleaning device into the reaction vessel 2 when the reaction line 1 is stopped, sucking up the reaction liquid 4 after the reaction, and then injecting cleaning water. be exposed. The reaction liquid 4 in the reaction container 2 is passed through the suction nozzle 8
is inserted into the reaction vessel 2, the inside of the trap bottle 15 is evacuated by the vacuum pump 16, and the solenoid valve 14 is opened to be pulled up. The cleaning water is injected from the injection nozzle 9 by feeding the cleaning water 17 with the water supply pump 18 and opening the solenoid valve 19. and.

By arranging a plurality of pulling nozzles 8 and injection nozzles 9, cleaning can be performed multiple times.

The degree of contamination of the washing water 20 injected into the reaction vessel 2 decreases as the stage progresses. Naturally, the amount of dirt in the reaction container 2 decreases accordingly. When the amount of dirt becomes less, water for water blanking is injected from the injection nozzle 11, and then the reaction line 1 rotates and when it crosses the optical axis 7, the water blank value is measured.

In this way, by measuring the water blank each time after washing the reaction container 2 and before using it for a new analysis, even if the reaction container 2 becomes scratched, cloudy, dirty, etc., it can be used as a standard. This allows data drift and level differences to be prevented. Since this water blank water serves as a standard, it is necessary to use clean water such as distilled water. Since the reaction vessel 2 is also being cleaned in the later stages, air bubbles tend to adhere to it. If the water blank water is vigorously discharged from the injection nozzle 11, air bubbles may be drawn in and adhere to the inner wall of the reaction vessel 2. As a preventive measure, the injection nozzle 11 is made a little longer to lower the discharge flow velocity to prevent air bubbles from being drawn in. However, although cleaning can be performed four times in FIG. 1, it is not possible to remove air bubbles that adhered to the inner wall of the reaction vessel 2 before the water blank water injection. Therefore, in this embodiment, in order to remove the bubbles, a bubble removing member 10 is attached to the tip of the suction nozzle in the cycle before the water blank water is injected, and the bubble removing member 10 is inserted to suck up the cleaning water 20. I made it.

FIG. 2 is a perspective view showing one embodiment of the bubble removing member 10. The bubble removing member 10 is configured to contact and rub against the inner wall of the reaction vessel 2 or to move near the inner wall,
When the suction nozzle is inserted into the reaction container 2, the reaction container 2
Air bubbles attached to the inner wall of the tube are either crushed and eliminated, or are pushed downward and sucked up into the suction nozzle and removed.

The reaction vessel 2 in which the water blank was measured is connected to the suction nozzle 1
2, the water blank water is sucked up and further dried by a suction nozzle equipped with a residual water removal member 13,
Used for analysis of new samples.

〔Effect of the invention〕

According to the present invention as described above, it is possible to prevent air bubbles from adhering to the wall surface of the reaction vessel when water blank water is injected into the reaction vessel, so water blank measurements can be performed accurately and data reliability can be improved. It has the effect of helping you improve.

[Brief explanation of the drawing]

FIG. 1 is a configuration diagram showing one embodiment of the reaction vessel cleaning device of the present invention, and FIG. 2 is a perspective view showing one embodiment of the bubble removing member of FIG. 1. DESCRIPTION OF SYMBOLS 1... Reaction line, 2... Reaction container, 8... Suction nozzle, 9... Injection nozzle, 1o... Air bubble removal member, 11... Water blank water injection nozzle.

Claims (1)

[Claims] 1. A reaction line that is repeatedly moved and stopped, a reaction container constituting the reaction line, and a suction nozzle are provided, the suction nozzle is inserted into the reaction container, and the contents of the container are sucked up,
Washing the reaction vessel while repeating injection of washing water,
In a reaction container cleaning device for an automatic analyzer that enables water blank measurement by injecting water for water blank measurement into the washed reaction container, before measuring the water for water blank measurement, A reaction vessel cleaning device characterized in that a suction nozzle equipped with a bubble removing member that rubs against an inner wall or moves near the inner wall is inserted into the reaction vessel.