JPH01203977A - Cold storage box for biochemical analysis apparatus - Google Patents

Abstract

PURPOSE:To prevent infiltration of the outdoor air into a cold storage box as far as possible and to improve the cooling efficiency of the cold storage box at the time of exchanging a test film cassette for biochemical analysis by constituting the cold storage box of a body having a heat insulating structure and a cap body which is divided to plural pieces and the respective pieces of which are independently freely openable and closable and have the heat insulating structure. CONSTITUTION:The wall parts of the cold storage box 6 except the front face thereof has the heat insulating structure. The caps 605 which cover respective through-holes 603 are provided on the upper side of a supporting plate 604 on the front face of the cold storage box 6. The caps 605 are provided respectively independently and freely openably and closably around the axes. Further, the caps 605 are so provided that the adjacent caps come into tight contact with each other. These caps are constituted in such a manner that the entire part covers integrally the front face of the cold storage box 6. In addition, the cap 605 has the insulating structure like the wall part. The infiltration of the outdoor air into the cold storage box is thereby prevented as far as possible at the time of exchanging a test film cassette for biochemical analysis with the fresh cassette. The degradation in the performance of the test films by contact of the other cassette with the atmosphere of a high temp. and high humidity is thus prevented.

Description

[Detailed description of the invention] [Industrial application field] The present invention relates to a cold storage for a biochemical analyzer.

More specifically, the present invention relates to a refrigerator for a biochemical analyzer that accommodates a plurality of cassettes for storing test films for biochemical analysis and has a high cooling effect.

[Prior art and its problems] In current medical care, biological body fluids (whole blood, plasma, serum, urine, saliva, etc.) are used for accurate diagnosis and appropriate treatment.
Clinical chemistry tests that perform quantitative analysis of various components contained in these substances are gaining importance.

For this purpose, it is necessary to quickly perform a large number of clinical chemistry tests, and the above quantitative analysis is carried out at medical institutions, laboratories, etc.
What is needed is a method and apparatus that is simple, automatic, and continuous.

For the above-mentioned quantitative analysis, there are biochemical analysis methods that use, for example, multilayer analytical test films that use spectroscopic measurements and analytical test films that measure ion activity using ion-selective electrodes. It has been proposed and in some cases put into practice.

Quantitative analysis using the above-mentioned multilayer analysis test film (hereinafter sometimes referred to as r-colorimetric test film) is as follows:
After spotting a small amount of the liquid to be tested on a colorimetric test film, this is kept at a constant temperature (incubation) for a predetermined period of time to cause a color reaction between the target component in the liquid to be tested and the reagent in the colorimetric test film. The objective component is quantitatively analyzed by measuring the color density spectrophotometrically.

In addition, in quantitative analysis using an analytical test film using an ion-selective electrode, a test liquid and a reference liquid are applied to each of the ion-selective layers of a sheet-shaped ion-selective electrode that are electrically separated from each other. The ionic activity of the test liquid is measured by measuring the potential difference between ion selective electrodes while the liquids are electrically connected to each other.

In order to automatically and continuously perform analysis using these test films for biochemical analysis, it is convenient to use long films in which these test films for biochemical analysis are constructed in a continuous manner. be. It is conceivable that such a long test film for biochemical analysis is stored in a cassette case and loaded into a biochemical analyzer.

On the other hand, test films for biochemical analysis need to be stored at low temperature and low humidity, preferably at a temperature of 5° C. or less and a humidity of 25% RH or less, from the time of manufacture until the time of use, in order not to reduce analytical accuracy.

Therefore, it is necessary for the biochemical analyzer to be equipped with a cold storage to store the test film cassettes for biochemical analysis at low temperature and low humidity, and the applicant has proposed a biochemical analyzer equipped with a cold storage. (Patent application 1986-176563)
issue).

By the way, since analytical measurements are usually performed on multiple items, not just one item, the cold storage stores a plurality of test film cassettes for biochemical analysis corresponding to multiple measurement items. Each time the test film is consumed, it is necessary to replace the test film cassette for biochemical analysis with a new one. Since the consumption of test film for biochemical analysis is not uniform for all the cassettes in the refrigerator, the lid of the refrigerator must be opened every time the cassette is replaced, and at that time, the outside air is forced into the refrigerator. It enters the refrigerator and increases the temperature and humidity of the air inside the refrigerator. When the temperature and humidity inside the cold storage increases, the test films of other test film cassettes for biochemical analysis existing in the cold storage deteriorate and the analysis accuracy decreases, and the temperature and humidity of the cold storage become low again. The energy required for this also increases.

[Object of the Invention] The object of the present invention is to prevent outside air from entering the cold storage when replacing a used cassette of a test film for biochemical analysis with a new test film cassette for biochemical analysis in the cold storage. The present invention is designed to prevent this as much as possible, to prevent other cassettes in the cold storage from coming into contact with a high temperature and high humidity atmosphere, thereby preventing the performance of the test film from deteriorating, and to improve the cooling efficiency of the cold storage. The purpose of the present invention is to provide a cold storage for chemical analysis equipment.

[Summary of the Invention] The present invention is a refrigerated box for a biochemical analyzer that stores a plurality of cassettes storing test films for biochemical analysis in parallel. The refrigerator for a biochemical analyzer is characterized by comprising a lid body which is divided into a plurality of parts in a direction, each of which can be opened and closed independently, and has a heat insulating structure.

A preferred embodiment of the present invention resides in the above-mentioned cold storage for a biochemical analyzer, characterized in that the lid is provided for each cassette.

A preferred embodiment of the present invention resides in the above-mentioned cold storage for a biochemical analyzer, characterized in that one lid is provided for an arbitrary number of cassettes.

A preferred embodiment of the present invention resides in the above-mentioned cold storage for a biochemical analyzer, characterized in that a lid is further provided on the outside of the divided lid to cover the entire upper surface of the cold storage.

A preferred embodiment of the present invention resides in the above-mentioned cold storage for a biochemical analyzer, characterized in that the lid is rotatably attached to the main body at all times.

A preferred embodiment of the present invention resides in the above-mentioned cold storage for a biochemical analyzer, characterized in that the lid is provided so as to be openable and closable by sliding in the horizontal direction with respect to the main body.

[Detailed description of the invention] The invention will now be described with reference to the accompanying drawings.

FIG. 1 is a perspective view showing an example of a colorimetric biochemical analyzer having a cold storage for a biochemical analyzer according to the present invention.

The illustrated biochemical analyzer 1 is equipped with a transparent lid 2, and when the lid 2 is opened, the test liquid described below, a long test film 31 in the form of a long tape, etc. are inserted into the device 1. It is adapted to contain and retrieve. This device 1 is equipped with a test liquid storage means 4 that stores test liquids such as serum and urine in a circular arrangement. It is taken out and spotted by the spotting means 5. The long test film 31 is made of multiple types of long films corresponding to the measurement items, such as containing a reagent that exhibits a color reaction with only the specific chemical component or tangible component to be measured in the test liquid. A test film 31 is prepared. The unused portion of the long test film 31 is wound inside the unused film cassette 30, and the portion used for the above measurement is wound inside the used film cassette 40. Also, in the center of the roll 41 in the used film cassette 40, a motor is used to rotate the long test film 31 for pulling out the film 31 from the unused film cassette 30 after storing it in the device 1, as will be described later. A hole 42 is provided for engaging the shaft. The long test film 31 is stored in the apparatus 1 while being wound around the cassette 30, 40. The unused film cassette 30 and the used film cassette 40 are separated as shown in this figure. In order to be able to measure multiple items at the same time using this device 1, a cold box 6 serving as a test film storage means is configured to store unused portions of a plurality of long test films 31 in parallel. has been done.

The spotting means 5 has a spotting nozzle 7 at its tip, is moved in the direction in which the rail 8 extends by a moving means 9 placed on the rail 8, takes out the test liquid from the test liquid storage means 4, It is spotted onto a long test film 31 pulled out from inside the cold storage 6 as described later. The moving means 9 is also configured to move the spotting means 5 in the vertical direction, and when the moving means 9 moves the spotting means 5 in the left and right direction in which the rail 8 extends, the spotting means 5 is moved vertically. The means is in an elevated position, and is lowered when taking out the liquid to be tested, spotting it, and cleaning as described later.

After the spotting nozzle 7 is spotted on the test film, it is cleaned by a nozzle cleaning unit 10 placed between the cold storage 6 and the test liquid storage means 4 in close proximity to both, and then used for the next spotting. Reused.

The spotted test film is incubated in an incubator as described below, and measured by a photometric means.

Control of the overall operation of the device 1, processing of measurement data, etc. are performed by a circuit section 11 and a computer 12 connected to this circuit section 11.
This is done by The operation/display section 13 provided on the front side of the circuit section 11 is equipped with a power switch for the device 1, an ammeter for monitoring the current consumption in the device 1, and the like. The computer 12 includes a keyboard 14 that gives instructions to the device 1, a CRT display 15 that displays auxiliary information for instructions, measurement results, etc., a printer 16 that prints out measurement results, and a computer that gives various instructions to the device 1. A floppy disk device 1 that accommodates a floppy disk for storing instructions and measurement result data, etc.
7 is provided.

FIG. 2 is a plan view of the main parts of the biochemical analyzer 1 whose perspective view is shown in FIG.

The cold storage 6 is configured such that the spotting positions 22 of all the test films pulled out from there are arranged in a straight line, and the nozzle cleaning section 10 and the inside of the test liquid storage means 4 are located on this straight line. The inspection target extraction positions 4b are arranged in such a manner that the inspection target extraction positions 4b are arranged.

The test liquid accommodating means 4 is configured to accommodate a plurality of test liquids in a substantially circularly arranged storage section 4a. In addition, this test liquid storage means 4 has a configuration in which a storage portion 4a arranged in a roughly circular shape is rotated.
Among the test liquids stored in the test liquid, the test liquid to be used for the next measurement is automatically rotated by a rotating means (not shown) so that the test liquid to be used for the next measurement is located at the take-out position 4b. In order to prevent deterioration due to evaporation of the test liquid stored in the storage part 4a, the depositing position 4 is
A lid (not shown) is placed over the accommodating portions 4a other than b.

The spotting means 5 is moved in the direction in which the rail extends by a moving means 9 mounted on the rail 8, takes out the liquid to be tested from the salt extraction position 4b, and deposits it on the long test film 31 at the spotting position 22.
Dot on.

FIG. 3 shows a main part of a cross section taken along line xx' in FIG. 2.

The long test film 31 is divided into an unused test film cassette 30 and a used test film cassette 4.
0 and loaded into the device. The unused test film cassette 30 is stored in a cold storage 6 whose interior is temperature-controlled at, for example, 4° C., and the used film cassette 40 is stored in the winding chamber 18. If the unused portion of the long test film 31 is stored in the unused test film cassette 30 in this manner, the unused long test film 31 can be stored in the cold storage 6 without being touched. The cold storage box 6 (details will be described later) is surrounded by a cold storage wall using a heat insulating material, and one side of this cold storage wall has the cold storage box 6
A cooling/dehumidifying device 19 is attached to keep the inside at a predetermined low temperature and low humidity, and a fan 20 circulates the air inside the cold storage 6.

As mentioned above, the used film cassette 4o is placed in the winding chamber 1.
8, this used film cassette 40
A rotating shaft of a winding motor 53, which is a conveying means for the long test film 31 provided in the winding chamber 18, is engaged with the hole 42 provided in the center of the inner roll 41. As the long test film 31 rotates, the long test film 31 is transferred from the unused test film cassette 30 to the drawer opening 60 of the cold storage 6.
8 and wound up into a used test film cassette 40.

If the used part of the long test film 31 is stored in the used film cassette 4o in this way, the used film that has been spotted with the test liquid and soiled can be removed from the apparatus 1 without touching it. can be disposed of, etc. Regarding this disposal, instead of winding the used film into the used test film cassette 40, the used film cassette 4o is removed and the winding chamber 1
A box that can be attached to and removed from the device 1 for storing the film is provided in the section 8, and a cutter for cutting the used film is placed near the sunrise in the winding chamber 18, and the used film is cut. The used film stored in the box can be taken out from the device 1 without touching the used film, and the used film can be disposed of. You can. In this case, the test film may be transported by providing transport rollers that sandwich and transport the test film.

An incubator 55 is disposed at the exposed portion of the long test film 31 between the unused film cassette 30 and the used film cassette 40, and the incubator 55 holds the film therein and allows the film to pass through it one after another. A side light section 57 is disposed for measuring the optical density due to the color reaction between the long test film 31 and the test liquid.

The long test film 31 is intermittently pulled out from the cold storage 6 by the rotation of the motor 53, and is intermittently fed to the left in the figure. rise in the direction. When the long test film 31 is moved, the upper lid 55a descends in the right direction of the arrow and pushes the long test film 31. Next, the shutter 54 that was blocking the nozzle insertion hole 55b of the upper lid 55a moves to the right in the figure, and then the nozzle 7 descends as shown in the figure and is placed on the long test film 31 through the nozzle insertion hole 55b. The liquid is applied. Furthermore, the shutter 54 moves to the left to open the nozzle insertion hole 55b.
The inside of the incubator is kept at a predetermined temperature (for example, 3
Keep at 7℃). The part of the film on which the test liquid has been applied and developed (the part shown with diagonal lines in FIG. 3) is kept in this incubator 55 for a predetermined period of time (for example, 4 minutes).
Maintained at constant temperature. After or during the incubation, the photometric section 57 measures the optical density of the portion of the long test film 31 where the spotting has been performed. This density measurement is performed by irradiating the film 31 with light containing a preselected wavelength emitted from the light irradiation means 57a and detecting the reflected light from the film 31 with the photodetector 57b.

When the spotting, incubation, and measurement of one liquid to be tested are completed in this way, the next liquid to be tested can be spotted. The long test film 31 remains in the incubator even after the above-mentioned measurement is completed, and the film portion to be used for the next analysis is placed at the spotting position immediately before the spotting for the next analysis. will be transferred to.

FIG. 4 is a perspective view showing an example of a cold storage box for a biochemical analyzer according to the present invention, FIG. 5 is a sectional view taken along the line nn in FIG. 4, and FIG. It is a sectional view taken along the line ■-■.

4 to 6, a plurality of unused film cassettes 30 storing test films for biochemical analysis are placed in the cold storage 6 in a horizontal direction in FIG. They are housed in parallel.

A perspective view of an example of the unused film cassette 30 is shown in FIG. In FIG. 7, an unused film cassette 30 has a test film 31 inside a thick plate-shaped rectangular parallelepiped case with a substantially square outer shape and a thickness slightly larger than the width of the test film 31. The test film 31 is stored in a rolled form, and a take-out port 33 for the test film 31 protrudes from the case body.

The cassette case as described above can be made by a method known per se. For example, the case and the lid of the cassette case are made separately, and after loading an unused test film, the case and the lid are fitted together. The case may be fixed by engagement, screwing, adhesion, or other means, or the case and the lid may be connected at one side of the lid and the connecting portion may be formed into a hinge structure.

A reel (not shown) for winding the test film 31 in a roll inside the unused film cassette 30
Wrapping the test film 31 with a radius of curvature that is too small is undesirable because there is a risk that the test film 31 will be cranked, so the diameter should not be made too small.

Generally, it is preferred that the diameter is 40 to 80 mm. Moreover, the inside of the reel has a suitable locking part to prevent the test film from being taken out more than necessary.

Cassette cases and reels are generally made of various thermoplastic resins, such as polyolefin resins such as polyethylene and polypropylene, polystyrene, high-impact polystyrene, styrene acrylonitrile resins, styrene resins such as ABS resins, vinyl chloride resins, nylon, It can be easily manufactured from polyester, polycarbonate, polyacetal, and other resins using the techniques used to manufacture audio and video cassette tapes.

An example of test film 31 is a colorimetric test film having a multilayer analytical element as shown in FIG. 8th
In the figure, a colorimetric test film 31 has a reagent layer 312 laminated on a light-transmitting support 311, and a developing layer 313 laminated thereon. In dry analysis,
A liquid to be tested is spotted on the developing layer 313, the liquid to be tested spreads in the developing layer 313, and the component to be analyzed moves to the reagent layer 312 and reacts with the reagent contained in the reagent layer 312, resulting in color density. The change is measured by reflection photometry from the support 311 side, and the component to be analyzed in the test liquid is analyzed using the principle of colorimetry. The colorimetric slide test film 31 may further include a reflective layer, a light shielding layer, a filtration layer, and a registrant layer, if necessary.
Other layers known in the art may also be included, such as cation layers, water absorption layers, subbing layers, etc. Further, the developing layer and the reagent layer may be a single layer.

The structure itself of a multilayer analytical element as shown in FIG. Examples of the substance include polymers such as polyethylene terephthalate, polycarbonate of bisphenol A, polystyrene, and cellulose esters (eg, cellulose diacetate, cellulose triacetate, cellulose acetate propionate, etc.). The thickness of the support 311 is about 50 μm to about 30 μm.
It is preferably in the range of 0 μm, especially in the range of 80 to 200 μm, and conveniently its width is in the range of about 3 to 10 mm. Further, its length can be appropriately determined depending on the number of analyzes per roll, and although the number is not particularly limited, it is generally convenient to have an analysis area for 100 to 600 times.

The spreading layer 313 spreads the liquid to be inspected that is spot-supplied on its surface at a rate of approximately constant amount per unit area in the lateral (horizontal) direction without substantially unevenly distributing the components contained therein. It has a spreading effect. The expansion layer 313 is
It is composed of paper such as filter paper, woven fabrics, non-woven fabrics, etc. made from natural fibers, synthetic fibers, etc. Also, the deployment layer 3
13 may be made from a porous material consisting of particulate polymer.

The spreading layer 313 further includes a cellulose derivative, a wood-loving polymer such as polyvinylpyrrolidone, polyvinyl alcohol, and polyacrylamide, and a nonionic, cationic, anionic, or amphoteric surfactant in order to control the spreading of the test liquid. It can be impregnated with various surfactants, suitable buffers for stable analysis, etc.

Reagent layer 312 contains a suitable reagent capable of reacting with the analyte to produce a colorimetrically detectable change in color density. The reagent layer 312 is preferably one in which one or more reagents are dispersed in a hydrophilic colloid such as gelatin, gelatin derivative, polyvinyl alcohol, polyacrylamide, or polyvinylpyrrolidone that acts as a binder.

As another example of the test film in the present invention, a test film having an ion-selective electrode can also be used.

In Figs. 4 to 6, the walls (side walls and bottom wall) other than the top surface of the cold storage box 6 have a heat insulating structure.

The heat insulating structure can employ a structure known per se, for example, a structure in which the entire structure is made of a heat insulating material, or a structure in which a double wall structure is formed and a cavity therein is filled with a heat insulating material.

The inner surface of the bottom wall 601 is provided with grooves 602 corresponding to the number of cassettes 30 accommodated in the cold storage 6, into which a part of the bottom portion of the cassettes 30 is fitted when the cassettes 30 are vertically erected. 6 years old
The width of 02 is preferably set to approximately approximate the thickness of cassette 30 so that cassette 30 does not move substantially laterally within groove 602. The length of the groove 602 is cassette 3.
The length of the side of the bottom part of cassette 3 is approximately approximated to that of cassette 3.
Preferably, the groove 602 is set such that the groove 602 does not move substantially laterally within the groove 602. There are usually 10 to 30
However, this number is not particularly limited.

A support plate 604 is provided above the cold storage 6 and has a through hole 603 that is large enough to allow the cassette 30 to pass in a direction parallel to the plane of the cassette 30 and supports the cassette on its side surface. The inside of the cold storage 6 is substantially filled with through holes 60.
The support plate 60 allows air to communicate with the outside only through 3.
It is preferable to provide 4. The width and length of the through hole 603 are preferably set so that the cassette 30 does not substantially move laterally. The upper surface of the support plate 604 is preferably provided so as to match the upper end surface of the cassette 30 when the cassette 30 is fitted into the groove 602.

Each through hole 6 is provided on the upper side of the support plate 604 on the upper surface of the cold storage 6.
A lid 605 that covers the through hole 603 is provided for each hole 603. The winter clothes 605 are provided so as to be able to be opened and closed independently using a shaft 606 as a fulcrum. The winter clothes 605 are provided so that adjacent lids are in close contact with each other, and are configured so as to cover the upper surface of the cold storage box 6 as a whole. The lid 605 has a heat insulating structure like the wall.

At the upper end of one side wall 607 of the cold storage 6, there is a cassette 30
A drawer opening 6 for inserting the film outlet 33 of
08 is provided. The test film 31 is taken out from the take-out port 33.

A cooling dehumidifier 19 is attached to the outside of the side wall 609 facing the side wall 607, and is capable of cooling and dehumidifying the air inside the cold storage box 6. Cassette 30 in cold storage 6
A space 610 is provided between the space 610 and the side wall 609, and air can freely circulate between the space 610 and the gap 611 between each cassette. By providing this, it is possible to circulate the air in the cold storage 6 and maintain all the cassettes at a uniformly low temperature and low humidity.

Since the cold storage 6 is configured as described above, the inside of the cold storage 6 is substantially airtight, and the air inside the cold storage 6 can be maintained at a low temperature and low humidity, and the cassette 30 can be replaced. When replacing the cassette, only the lid 605 of the cassette to be replaced can be opened, thereby minimizing the entry of outside air into the cold storage 6 and increasing the temperature and humidity of the air inside the cold storage 6. This can be prevented to a minimum. Further, since the cassette 30 is fitted into the groove 602 and the through hole 603 so as not to move substantially, each gap 611 is in a substantially independent state, and even if the lid 605 is opened at the Changes in the temperature and humidity of the internal air caused by outside air entering from the cassettes have almost no effect on the cassettes in the open locations, making it possible to obtain highly integrated analysis results in a stable state.

In FIGS. 4 to 6, an example is shown in which the lid 605 is provided for each cassette 30, but the lid 605 does not necessarily need to be provided for each cassette, and may be provided for any number of cassettes 30, for example, two. , may be provided for every three pieces, or may be provided for any different number of pieces.

Furthermore, a single lid (not shown) may be further provided above the lid 605 to cover the entire top surface of the cold storage 6, so that the top surface of the cold storage 6 has a double lid structure.

In addition, as shown in FIG.
5 may be attached to the upper end of the side wall 609 with a hinge so that it can be opened and closed, or the lid 605 may be slid in the left-right direction in FIG. 4 to be opened and closed.

[Effects of the Invention] The cold storage for biochemical analysis equipment of the present invention can accommodate a plurality of cassettes for storing test films for biochemical analysis, thereby saving space and improving its cooling efficiency. At the same time, when replacing used test film cassettes for biochemical analysis in the cold storage with new test film cassettes for biochemical analysis, it is possible to prevent outside air from entering the cold storage as much as possible. Preventing other cassettes in the refrigerator from coming into contact with a high temperature, high humidity atmosphere and reducing the performance of the test film, and
It is a cold storage that can achieve the remarkable effect of improving the cooling efficiency of the cold storage.

[Brief explanation of the drawing]

FIG. 1 is a perspective view showing an example of a colorimetric biochemical analyzer having a refrigerator for biochemical analyzers according to the present invention, and FIG. 2 shows the main components of the biochemical analyzer l shown in FIG. FIG. 3 is a cross-sectional view taken along the line xx' in FIG. Figure 5 is a sectional view taken along line nn in Figure 4, Figure 6 is
Fig. 4 is a sectional view taken along the line m-■, Fig. 7 is a perspective view of an example of an unused film cassette stored in a cold storage for a biochemical analyzer of the present invention, and Fig. 8 is an example of a colorimetric test film. FIG. l: biochemical analyzer, 2: transparent lid 4: test liquid storage stage, 5: test liquid spotting means, 6: cold storage, 7: spotting nozzle, 8: rail,
9: Transportation means, 10: Nozzle cleaning section, 11: Circuit section, 12: Computer, 13: Operation/display section, 14: Keyboard, 15: CRT display, 16: Printer, 17: Floppy disk device, 18: Winding Room, 19:
Cooling and dehumidifying device, 20: Fan, 22: Spotting position, 30: Unused film cassette, 31: Test film, 32: Side, 33: Output port, 311: Light-transparent support, 312
: reagent layer, 313: developing layer, 40: used film cassette 53: motor, 54: shutter, 55: incubator, 55a: upper lid, 55b: nozzle insertion hole, 57
: Photometry section, 57a: Light irradiation means, 57b: Photodetector, 6
01: Bottom wall, 602: Groove, 603: Through hole, 604: Support plate, 605: Lid,
606: Axis, 607: Side wall, 608: Hole, 609: Side wall, 610: Space, 611: Gap. Patent applicant Fuji Photo Film Co., Ltd. Agent Patent attorney Hata Yanagawa Figure 4 M6 Figure 7 Figure 8

Claims (1)

[Claims] 1. A cold storage for a biochemical analyzer that stores a plurality of cassettes for storing test films for biochemical analysis in parallel, the refrigerator having a main body having a heat insulating structure, and a plurality of cassettes divided in the parallel direction of the cassettes, each of which is independent. 1. A refrigerated box for a biochemical analyzer, comprising a lid body that can be opened and closed freely and has an insulating structure.