JPH0118885B2 - - Google Patents
Info
- Publication number
- JPH0118885B2 JPH0118885B2 JP11763680A JP11763680A JPH0118885B2 JP H0118885 B2 JPH0118885 B2 JP H0118885B2 JP 11763680 A JP11763680 A JP 11763680A JP 11763680 A JP11763680 A JP 11763680A JP H0118885 B2 JPH0118885 B2 JP H0118885B2
- Authority
- JP
- Japan
- Prior art keywords
- babesia
- parasites
- water
- antigen
- soluble
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000427 antigen Substances 0.000 claims description 88
- 102000036639 antigens Human genes 0.000 claims description 88
- 108091007433 antigens Proteins 0.000 claims description 88
- 201000008680 babesiosis Diseases 0.000 claims description 63
- 241000223836 Babesia Species 0.000 claims description 56
- 210000003936 merozoite Anatomy 0.000 claims description 36
- 244000045947 parasite Species 0.000 claims description 34
- 210000002966 serum Anatomy 0.000 claims description 33
- 241001465754 Metazoa Species 0.000 claims description 32
- 229960005486 vaccine Drugs 0.000 claims description 21
- 238000000338 in vitro Methods 0.000 claims description 18
- 241000223838 Babesia bovis Species 0.000 claims description 14
- 238000001727 in vivo Methods 0.000 claims description 14
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 230000001681 protective effect Effects 0.000 claims description 12
- 239000000126 substance Substances 0.000 claims description 12
- 150000001720 carbohydrates Chemical class 0.000 claims description 11
- 102000004169 proteins and genes Human genes 0.000 claims description 11
- 108090000623 proteins and genes Proteins 0.000 claims description 11
- 208000015181 infectious disease Diseases 0.000 claims description 9
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 8
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 7
- 239000002671 adjuvant Substances 0.000 claims description 7
- 238000000760 immunoelectrophoresis Methods 0.000 claims description 7
- 241000223846 Babesia canis Species 0.000 claims description 6
- 241001466447 Babesia gibsoni Species 0.000 claims description 6
- 241000223776 Theileria equi Species 0.000 claims description 6
- 239000004382 Amylase Substances 0.000 claims description 5
- 102000013142 Amylases Human genes 0.000 claims description 5
- 108010065511 Amylases Proteins 0.000 claims description 5
- 241000223840 Babesia bigemina Species 0.000 claims description 5
- 241000593848 Babesia major Species 0.000 claims description 5
- 241000223848 Babesia microti Species 0.000 claims description 5
- 235000019418 amylase Nutrition 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 5
- 241000223774 Babesia rodhaini Species 0.000 claims description 4
- 239000000975 dye Substances 0.000 claims description 4
- 241000223775 Babesia caballi Species 0.000 claims description 3
- 241001455947 Babesia divergens Species 0.000 claims description 3
- 239000003085 diluting agent Substances 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 238000001962 electrophoresis Methods 0.000 claims description 2
- 230000001939 inductive effect Effects 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 claims description 2
- 238000002372 labelling Methods 0.000 claims description 2
- 238000010186 staining Methods 0.000 claims description 2
- 241001314546 Microtis <orchid> Species 0.000 claims 1
- -1 n Species 0.000 claims 1
- 239000003223 protective agent Substances 0.000 claims 1
- 238000000034 method Methods 0.000 description 32
- 239000000463 material Substances 0.000 description 23
- 210000004369 blood Anatomy 0.000 description 20
- 239000008280 blood Substances 0.000 description 20
- 210000003743 erythrocyte Anatomy 0.000 description 19
- 238000002360 preparation method Methods 0.000 description 18
- 230000000890 antigenic effect Effects 0.000 description 16
- 210000004027 cell Anatomy 0.000 description 16
- 241000283690 Bos taurus Species 0.000 description 11
- 230000004071 biological effect Effects 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000010171 animal model Methods 0.000 description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 208000030852 Parasitic disease Diseases 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- 208000009182 Parasitemia Diseases 0.000 description 5
- 230000036760 body temperature Effects 0.000 description 5
- 235000014633 carbohydrates Nutrition 0.000 description 5
- 239000012531 culture fluid Substances 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- 230000002458 infectious effect Effects 0.000 description 5
- 235000018102 proteins Nutrition 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 239000012228 culture supernatant Substances 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000000704 physical effect Effects 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 241000224016 Plasmodium Species 0.000 description 3
- 244000309466 calf Species 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 238000006386 neutralization reaction Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 238000002255 vaccination Methods 0.000 description 3
- 102000009123 Fibrin Human genes 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- UZQJVUCHXGYFLQ-AYDHOLPZSA-N [(2s,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-4-[(2r,3r,4s,5r,6r)-4-[(2s,3r,4s,5r,6r)-3,5-dihydroxy-6-(hydroxymethyl)-4-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-3,5-dihydroxy-6-(hy Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O)O[C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O)O[C@H]1CC[C@]2(C)[C@H]3CC=C4[C@@]([C@@]3(CC[C@H]2[C@@]1(C=O)C)C)(C)CC(O)[C@]1(CCC(CC14)(C)C)C(=O)O[C@H]1[C@@H]([C@@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O[C@H]4[C@@H]([C@@H](O[C@H]5[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O5)O)[C@H](O)[C@@H](CO)O4)O)[C@H](O)[C@@H](CO)O3)O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O UZQJVUCHXGYFLQ-AYDHOLPZSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000004709 cell invasion Effects 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 229950003499 fibrin Drugs 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000003071 parasitic effect Effects 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 229940124856 vaccine component Drugs 0.000 description 2
- HZLHRDBTVSZCBS-UVJJDBRNSA-N 4-[(e)-(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline;hydrochloride Chemical compound Cl.C1=CC(=N)C(C)=C\C1=C(C=1C=C(C)C(N)=CC=1)/C1=CC=C(N)C=C1 HZLHRDBTVSZCBS-UVJJDBRNSA-N 0.000 description 1
- 241000238876 Acari Species 0.000 description 1
- 241000606646 Anaplasma Species 0.000 description 1
- 206010003964 Babesia infections Diseases 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 241000223834 Piroplasmida Species 0.000 description 1
- 102000007327 Protamines Human genes 0.000 description 1
- 108010007568 Protamines Proteins 0.000 description 1
- 241000238680 Rhipicephalus microplus Species 0.000 description 1
- 241000283984 Rodentia Species 0.000 description 1
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000007982 barbital buffer Substances 0.000 description 1
- 229960000796 barbital sodium Drugs 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000001085 differential centrifugation Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 201000001505 hemoglobinuria Diseases 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 210000004201 immune sera Anatomy 0.000 description 1
- 229940042743 immune sera Drugs 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 230000002163 immunogen Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000013365 molecular weight analysis method Methods 0.000 description 1
- QYSGYZVSCZSLHT-UHFFFAOYSA-N octafluoropropane Chemical compound FC(F)(F)C(F)(F)C(F)(F)F QYSGYZVSCZSLHT-UHFFFAOYSA-N 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 229950008679 protamine sulfate Drugs 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/130,481 US4596707A (en) | 1979-04-30 | 1980-03-31 | Babesia parasite antigen useful in vaccine and diagnostic reagent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS56140925A JPS56140925A (en) | 1981-11-04 |
| JPH0118885B2 true JPH0118885B2 (enrdf_load_stackoverflow) | 1989-04-07 |
Family
ID=22444889
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP11763680A Granted JPS56140925A (en) | 1980-03-31 | 1980-08-25 | Water soluble antigen idiosyncratic to babesia group parasite |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPS56140925A (enrdf_load_stackoverflow) |
| ZA (1) | ZA802384B (enrdf_load_stackoverflow) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03135097A (ja) * | 1989-10-19 | 1991-06-10 | Sanyo Electric Co Ltd | 電気機器の取付装置 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4752054B2 (ja) * | 2005-08-25 | 2011-08-17 | 国立大学法人帯広畜産大学 | バベシア・ギブソニ（Ｂａｂｅｓｉａｇｉｂｓｏｎｉ）の分泌抗原１タンパク質及びこのタンパク質をコードするＤＮＡ、並びにそれらの利用 |
-
1980
- 1980-04-21 ZA ZA00802384A patent/ZA802384B/xx unknown
- 1980-08-25 JP JP11763680A patent/JPS56140925A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03135097A (ja) * | 1989-10-19 | 1991-06-10 | Sanyo Electric Co Ltd | 電気機器の取付装置 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS56140925A (en) | 1981-11-04 |
| ZA802384B (en) | 1981-05-27 |
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