JPH01187082A - Production of astaxanthin-rich chlorophyceae - Google Patents
Production of astaxanthin-rich chlorophyceaeInfo
- Publication number
- JPH01187082A JPH01187082A JP63012328A JP1232888A JPH01187082A JP H01187082 A JPH01187082 A JP H01187082A JP 63012328 A JP63012328 A JP 63012328A JP 1232888 A JP1232888 A JP 1232888A JP H01187082 A JPH01187082 A JP H01187082A
- Authority
- JP
- Japan
- Prior art keywords
- astaxanthin
- culture
- chlorophyceae
- chlorella
- salts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 title claims abstract description 45
- 235000013793 astaxanthin Nutrition 0.000 title claims abstract description 45
- 239000001168 astaxanthin Substances 0.000 title claims abstract description 45
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 title claims abstract description 45
- 229940022405 astaxanthin Drugs 0.000 title claims abstract description 45
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 7
- 241000196319 Chlorophyceae Species 0.000 title abstract 6
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims abstract description 14
- 241000195663 Scenedesmus Species 0.000 claims abstract description 14
- 159000000000 sodium salts Chemical class 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 5
- 241000195585 Chlamydomonas Species 0.000 claims abstract 2
- 241000790338 Dictyococcus Species 0.000 claims abstract 2
- 241000180182 Protosiphon Species 0.000 claims abstract 2
- 241000195628 Chlorophyta Species 0.000 claims description 23
- 241000195493 Cryptophyta Species 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 6
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 claims description 4
- 150000003297 rubidium Chemical class 0.000 claims description 3
- 241000894007 species Species 0.000 claims description 2
- 241000168525 Haematococcus Species 0.000 claims 1
- 235000013305 food Nutrition 0.000 abstract description 5
- 230000015572 biosynthetic process Effects 0.000 abstract description 4
- 150000003839 salts Chemical class 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 239000001963 growth medium Substances 0.000 abstract description 3
- 159000000001 potassium salts Chemical class 0.000 abstract description 3
- 241000196169 Ankistrodesmus Species 0.000 abstract 1
- 241000180279 Chlorococcum Species 0.000 abstract 1
- 241000542911 Coelastrum Species 0.000 abstract 1
- 241000163983 Crucigenia Species 0.000 abstract 1
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 abstract 1
- 239000007858 starting material Substances 0.000 abstract 1
- 241000238557 Decapoda Species 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 230000002950 deficient Effects 0.000 description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 238000012136 culture method Methods 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 241000473391 Archosargus rhomboidalis Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 244000249214 Chlorella pyrenoidosa Species 0.000 description 2
- 235000007091 Chlorella pyrenoidosa Nutrition 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 238000013019 agitation Methods 0.000 description 2
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 2
- 235000013734 beta-carotene Nutrition 0.000 description 2
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 2
- 239000011648 beta-carotene Substances 0.000 description 2
- 229960002747 betacarotene Drugs 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 239000013028 medium composition Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 159000000005 rubidium salts Chemical class 0.000 description 2
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 235000016425 Arthrospira platensis Nutrition 0.000 description 1
- 240000002900 Arthrospira platensis Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000192700 Cyanobacteria Species 0.000 description 1
- 241000239366 Euphausiacea Species 0.000 description 1
- 206010016717 Fistula Diseases 0.000 description 1
- 241001268946 Homospora Species 0.000 description 1
- 208000008167 Magnesium Deficiency Diseases 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 241001442243 Mychonastes homosphaera Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 241000195647 [Chlorella] fusca Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000003570 biosynthesizing effect Effects 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 150000001805 chlorine compounds Chemical class 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000003890 fistula Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000003308 immunostimulating effect Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 235000004764 magnesium deficiency Nutrition 0.000 description 1
- 150000002823 nitrates Chemical class 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000001054 red pigment Substances 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 229910021654 trace metal Inorganic materials 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
この発明はアスタキサンチンを高濃度で含有する緑藻類
の生産方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] This invention relates to a method for producing green algae containing astaxanthin at a high concentration.
赤色色素のアスタキサンチンはカロチノイド系色素の一
種で、動物界に広く分布している。カニやエビ等の甲殻
類の赤味はこのアスタキサンチンであり、水産業界では
、カニやエビの殻を鯛、錦鯉、エビの色（赤色）あげに
用いている。食品業界では古くより、センベイ等にエビ
やカニをそのままか、粉末として添加し、風味付け、色
付けなどに利用しており、最近ではめん類、パン等にも
使われ始めている。いずれも色付け１色あげに効果のあ
るのはアスタキサンチンである。またアスタキサンチン
は、β−カロチンのようなビタミンＡ効力を持たないが
、最近ではβ−カロチンより強い免疫賦活作用および腫
瘍排除（縮少）作用も確認されており、有用色素として
の価値が高まりつつある。The red pigment astaxanthin is a type of carotenoid pigment that is widely distributed in the animal kingdom. Astaxanthin is responsible for the red color of crustaceans such as crabs and shrimp, and in the seafood industry, crab and shrimp shells are used to give the red color to sea bream, Nishikigoi, and shrimp. For a long time in the food industry, shrimp and crab have been added to foods such as Senbei, either as they are or as a powder, to add flavor and color, and recently they have also begun to be used in noodles, bread, etc. In both cases, astaxanthin is effective in increasing color by one color. Furthermore, although astaxanthin does not have the same vitamin A efficacy as β-carotene, it has recently been confirmed that it has a stronger immunostimulatory effect and tumor elimination (reduction) effect than β-carotene, and its value as a useful pigment is increasing. be.
しかし、アスタキサンチンの天然資源として現在利用さ
れているのは、主としてカニ、エビ、オキアミ等の殻で
あるが、これらに含まれるアスタキサンチン含量は低く
、乾燥物１ｇあたり０．１〜０．５醜ｇでしかなく、ま
た動物や人が利用（食）しやすいものではない。However, the shells of crabs, shrimp, krill, etc. are currently used as natural resources for astaxanthin, but the astaxanthin content contained in these is low, with 0.1 to 0.5 g of astaxanthin per 1 g of dry matter. Moreover, it is not something that animals or people can easily use (eat).
一方、緑藻類は通常アスタキサンチンは含有しないか、
含有しても極めて微量であることが定説になっている。On the other hand, green algae usually do not contain astaxanthin or
It is a well-established theory that even if it is contained, it is in extremely small amounts.
しかしいくつかの緑藻類は異常な生存環境下１例えば窒
素等の栄養欠乏環境下ではアスタキサンチンを生合成す
る。例えば緑藻へマドコツカス（Ｈａｅ＋５ａｔｏｃｏ
ｃｃｕｓ）はＮ欠乏培養で多量に、アスタキサンチンを
含有（２ｍｇ／ｇ）することが知られており、実験的に
培養され、エビや鯛の色あげ飼料として有効であると報
告されている。また現在、大量培養され、食品として利
用されている緑藻のクロレラやセネデスムスも、Ｎ欠乏
またはマグネシウム欠乏培養すればアスタキサンチンを
生合成することが知られており、このときのアスタキサ
ンチンの含有量はおおよそ０．５〜１．５ｍｇ／ｇであ
る。However, some green algae biosynthesize astaxanthin under abnormal survival conditions, such as environments lacking nutrients such as nitrogen. For example, Hae+5atococus
Ccus) is known to contain a large amount of astaxanthin (2 mg/g) in N-deficient culture, and has been experimentally cultured and reported to be effective as color-enhancing feed for shrimp and sea bream. In addition, the green algae Chlorella and Scenedesmus, which are currently cultivated in large quantities and used as food, are known to biosynthesize astaxanthin if cultured in N- or magnesium-deficient cultures, and the astaxanthin content at this time is approximately 0. .5 to 1.5 mg/g.
しかしながら上記の方法によって培養される緑藻類に含
まれるアスタキサンチンの含有量は必ずしも多いという
ことはできず、工業的な利用には適していないという問
題点があった。However, the content of astaxanthin contained in green algae cultured by the above method cannot necessarily be said to be high, and there is a problem that it is not suitable for industrial use.
この発明の目的は、上記問題点を解決するため、簡単な
培養方法により、アスタキサンチンを高濃度に含有する
緑藻類の生産方法を提供することである。An object of the present invention is to provide a method for producing green algae containing astaxanthin at a high concentration by a simple culture method in order to solve the above-mentioned problems.
この発明は、アスタキサンチンを生合成する緑藻類を、
ナトリウム塩、カリウム塩およびルビジウム塩から選ば
れる１種以上を０．２〜ＩＭ含む培地中で培養すること
を特徴とするアスタキサンチン高含有緑藻類の生産方法
である。This invention uses green algae that biosynthesize astaxanthin.
This is a method for producing green algae with a high content of astaxanthin, which is characterized by culturing in a medium containing 0.2 to IM of one or more selected from sodium salts, potassium salts, and rubidium salts.
本発明において培養の対象となる緑藻類は、アスタキサ
ンチンを生合成する緑藻類であって、表１に属するもの
があげられる。The green algae to be cultured in the present invention are green algae that biosynthesize astaxanthin, and include those listed in Table 1.
表１
緑藻類以外の藻類、例えば大量培養生産されている食用
藻のスピルリナ（藍藻類）やいわゆる海産クロレラ（分
類学上、緑藻類ではなく、クロレラとは別な藻類である
）では、いずれもＮ欠乏培養や本発明の培養法によって
もアスタキサンチンは生合成されない。Table 1 Algae other than green algae, such as Spirulina (blue-green algae), an edible algae that is produced in large quantities, and so-called marine chlorella (taxonomically speaking, it is not a green algae but a different algae from chlorella) are both N-deficient. Astaxanthin is not biosynthesized by culture or the culture method of the present invention.
上記緑藻類の中では、クロレラ、セネデスムスなどはす
でに食品として利用されているため、安全性の面から問
題はなく、しかも大量培養技術も確立しているので、本
発明に適用するのが好ましい。Among the above-mentioned green algae, Chlorella, Scenedesmus, etc. are already used as foods, so there is no problem from a safety perspective, and mass culture technology has been established, so it is preferable to apply them to the present invention.
このうちクロレラについては、クロレラ・フスカ（＝ク
ロレラピレノイドーサ）（Ｃｈｌｏｒａｌｌａ ｆｕｓ
ｃａ（＝Ｃｈｌｏｒｅｌｌａ ｐｙｒｅｎｏｉｄｏｓａ
））、クロレラ拳ゾーフインゲンシス（Ｃｈｌｏｒｅｌ
ｌａ ｚｏｆｉｎｇｉｅｎｓｉｓ）、クロレラ・ホモス
ポーラ（Ｃｈｌｏｒｅｌｌａ ｈｏｍｏｓｐｈａｅｒａ
）等がアスタキサンチンを生合成する。セネデスムスそ
の他の属についてはほとんどの種がアスタキサンチンを
生合成する。Of these, Chlorella fusca (= Chlorella pyrenoidosa)
ca (= Chlorella pyrenoidosa
)), Chlorella fistula
la zofingiensis), Chlorella homospora (Chlorella homosphaera)
) etc. biosynthesize astaxanthin. Most species of Scenedesmus and other genera biosynthesize astaxanthin.
本発明では上記のようなアスタキサンチンを生合成する
緑藻類を、ナトリウム塩、カリウム塩およびルビジウム
塩から選ばれる１種以上の塩を０．２〜ＩＭ含む培地中
で培養することにより、アスタキサンチンを生合成させ
、高濃度にアスタキサンチンを含有する緑藻類を生産す
ることができる。In the present invention, astaxanthin can be biosynthesized by culturing the green algae that biosynthesize astaxanthin as described above in a medium containing 0.2 to IM of one or more salts selected from sodium salt, potassium salt, and rubidium salt. It is possible to produce green algae containing astaxanthin at a high concentration.
上記の塩としては、塩化物、硝酸塩、硫酸塩などがあげ
られる。Examples of the above salts include chlorides, nitrates, and sulfates.
緑藻類の培養方法は、従来よりクロレラ、セネデスムス
等の培養に採用されている方法が適用可能である。As a method for culturing green algae, methods conventionally employed for culturing Chlorella, Scenedesmus, etc. can be applied.
クロレラやセネデスムスの大量培養は光（太陽光）照射
下で行われているのが大部分であるが。Most of the mass cultivation of Chlorella and Scenedesmus is carried out under light (sunlight) irradiation.
全く光を必要としない従属栄養培養も可能である。Heterotrophic culture, which requires no light at all, is also possible.
前者は開放攪拌培養、後者はタンク培養と一般に呼ばれ
ているが、いずれの培養においても本発明を適用するこ
とができる。これらの培養に際してナトリウム塩、カリ
ウム塩、ルビジウム塩等を０．２〜ＩＭ培地に添加すれ
ば１通常の培養に比べてクロレラやセネデスムス等の増
殖速度ははるかに低下する。このためアスタキサンチン
の生合成は促進されるが、生産量では緑藻の増加は期待
できない。したがってまず通常使用される培地（窒素源
、リン酸源、カリウム、マグネシウム、鉄および他の微
量金属元素を含む公知の培地）を使用し、炭酸ガス、酢
酸、ブドウ糖等を炭素源として開放攪拌培養またはタン
ク培養により大量培養し、その後ナトリウム塩、カリウ
ム塩、ルビジウム塩等を０．２〜ＩＭ濃度で添加し、培
養を続けるのがよい。The former is generally called open agitation culture, and the latter is generally called tank culture, but the present invention can be applied to either type of culture. If sodium salts, potassium salts, rubidium salts, etc. are added to the 0.2 to IM medium during these cultures, the growth rate of Chlorella, Scenedesmus, etc. will be much lower than in normal culture. As a result, astaxanthin biosynthesis is promoted, but an increase in green algae cannot be expected in terms of production. Therefore, first, a commonly used medium (known medium containing nitrogen source, phosphoric acid source, potassium, magnesium, iron, and other trace metal elements) is used, and open agitation culture is performed using carbon dioxide gas, acetic acid, glucose, etc. as a carbon source. Alternatively, it is preferable to carry out mass culture by tank culture, and then add sodium salt, potassium salt, rubidium salt, etc. at a concentration of 0.2 to IM, and continue culturing.
はぼ４日間でクロレラや、セネデスムス等の緑藻類はア
スタキサンチンを高濃度に含有するようになり、培養液
は赤色となる。この時、窒素欠乏あるいはマグネシウム
欠乏等の栄養欠乏状態を組合せることも可能であり、こ
れによりアスタキサンチンの生合成は若干促進され、培
養日数の短縮がみられる。Within four days, green algae such as Chlorella and Scenedesmus begin to contain astaxanthin at a high concentration, and the culture solution turns red. At this time, it is also possible to combine a nutritional deficiency state such as nitrogen deficiency or magnesium deficiency, which slightly accelerates astaxanthin biosynthesis and shortens the number of culture days.
こうして生産されたアスタキサンチン高含有緑藻類はア
スタキサンチンを４〜ｌｏ＋ｓｇ／ｇ含有し、その含有
量は栄養欠乏培養の４〜１０倍の高含有承である。得ら
れた藻体は従来の藻体処理と同様に処理され、アスタキ
サンチン高含有藻体としてそのまま利用されるか、ある
いは藻体からアスタキサンチンを抽出して利用される。The astaxanthin-rich green algae produced in this way contains astaxanthin from 4 to lo+sg/g, which is 4 to 10 times higher than that of the nutrient-deficient culture. The obtained algae is treated in the same manner as conventional algae treatment and used as is as algae containing high astaxanthin content, or astaxanthin is extracted from the algae and used.
以上の通り、本発明によれば、アスタキサンチンを生合
成する緑藻類を特定の塩類を含む培地で培養するように
したので、簡単な培養法により。As described above, according to the present invention, green algae that biosynthesize astaxanthin are cultured in a medium containing specific salts, so that a simple culture method can be used.
高濃度にアスタキサンチンを含有する緑藻類を生産する
ことができる。Green algae containing astaxanthin at high concentrations can be produced.
以下、本発明の実施例について説明する。 Examples of the present invention will be described below.
実施例１
セネデスムス・クロレロイズ（Ｓｃａｎｅｄｅｓｍｕｓ
ｃｈｌｏｒｅｌｌｏｉｄａｓ）を通常の培養液を用いて
、２Ｉ２容量の扁平フラスコにより予備培養した。培地
組成は表２の通りであり、培養条件は温度２８℃、蛍光
燈による連続照明（約１５０００Ｌｕｘ）、３容量％の
炭酸ガスを含む空気によってバブリングを行った。培養
開始濃度はＰＣＶ（ｌＱ中の生細胞量） ３ ｃｃ前後
であったが、６日間培養後ＰＣＶ ３２ｃｃとなった。Example 1 Scenedesmus chlorellois
chlorelloidas) was precultured in a 2I2 capacity flat flask using a normal culture medium. The medium composition was as shown in Table 2, and the culture conditions were a temperature of 28° C., continuous illumination with a fluorescent light (approximately 15,000 Lux), and bubbling with air containing 3% by volume of carbon dioxide gas. The starting concentration of the culture was around 3 cc of PCV (amount of living cells in 1Q), but after 6 days of culture, the PCV was 32 cc.
ついで遠心分離によりセネデスムスを収穫し、一部を乾
燥してアスタキサンチンの含有量を測定した。残りを２
分し、一方をＮ欠乏培養（表２の組成よりＫＮＯ，を除
いたもの）、もう一方を本発明によるナトリウム塩培地
（表２の組成にＮａＣＱｏ、５Ｍ［約３重量％濃度］を
加えたもの）によって４〜６日間培養した。培養開始時
の濃度はＰＣＶ １４ｃｃであった。培養温度、照明、
炭酸ガス濃度等は予備培養と同様である。培養を続ける
ことによって、いずれも培養液は赤色を示し、セネデス
ムスがアスタキサンチンを生合成していくのが確認され
た。Scenedesmus was then harvested by centrifugation, a portion was dried, and the astaxanthin content was measured. 2 remaining
One part was cultured with N deficiency (KNO was removed from the composition in Table 2), and the other was a sodium salt medium according to the present invention (NaCQo, 5M [approximately 3% by weight concentration] was added to the composition in Table 2). cultured for 4 to 6 days. The concentration at the start of culture was 14 cc of PCV. Culture temperature, lighting,
Carbon dioxide concentration, etc. are the same as in the preliminary culture. As the culture continued, the culture solution in both cases turned red, confirming that Scenedesmus was biosynthesizing astaxanthin.
培養終了時のセネデスムスの濃度、およびアスタキサン
チンの含有量は表３のとおりであり、本発明によってア
スタキサンチンの含有量はＮ欠乏培養と比べて、大なる
ものであった。The concentration of Scenedesmus and the content of astaxanthin at the end of the culture are shown in Table 3, and the content of astaxanthin according to the present invention was greater than that in the N-deficient culture.
表２ 培地組成
表３ アスタキサンチン含有量
Ｄｖ＝乾燥重量
実施例２
クロレラ・ゾーフインゲンシＸ’ （Ｃｈｌｏｒａｌｌ
ａｚｏｆｉｎｇｉｅｎｓｉｓ）を表４の培養液で予備培
養し、遠心分離により収穫したのち２分し、一方をＮ欠
乏培養（表４より（ＮＨ，）、ＣＯを除いたもの）、も
う一方を本発明によるカリウム塩培養（表４にＫｌ、Ｇ
Ｏ，５Ｍ〔約３．７重量％濃度〕を加えたもの）とし
、グルコースを炭素源としてタンク（暗所）培養を行っ
た。温度は２８℃、培養日数は４〜６日間であった。Table 2 Medium Composition Table 3 Astaxanthin Content Dv = Dry Weight Example 2
azofingiensis) was precultured with the culture solution shown in Table 4, harvested by centrifugation, and then divided into two halves, one of which was cultured with N deficiency (from Table 4, (NH,), CO was removed), and the other was cultured according to the present invention. Potassium salt culture (Table 4 shows Kl, G
O.5M (approximately 3.7% by weight concentration) was added), and tank (dark) culture was performed using glucose as a carbon source. The temperature was 28°C, and the number of culture days was 4 to 6 days.
培養開始濃度はＰＣＶ １８ｃｃ、終了時のｐｃｖは２
２〜２６ｅｅであった。The concentration at the start of the culture was 18cc PCV, and the PCV at the end was 2.
It was 2-26ee.
それぞれの培養で得られたクロレラ中のアスタキサンチ
ンの含有量を表５に示した。Table 5 shows the astaxanthin content in Chlorella obtained in each culture.
表４ 培地 表５ アスタキサンチン含有量 代理人 弁理士 柳 原 成Table 4 Medium Table 5 Astaxanthin content Agent: Patent attorney Sei Yanagi Hara
Claims (2)
ウム塩、カリウム塩およびルビジウム塩から選ばれる１
種以上を０．２〜１Ｍ含む培地中で培養することを特徴
とするアスタキサンチン高含有緑藻類の生産方法。(1) Green algae that biosynthesize astaxanthin are combined with 1 selected from sodium salt, potassium salt, and rubidium salt.
1. A method for producing green algae with a high content of astaxanthin, which comprises culturing the algae in a medium containing 0.2 to 1M of the above species.
マトコッカス、アンキストロデスムス、クロレラ、クロ
ロコックム、ケラストルム、クルシゲニア、ディクティ
オコッカス、セネデスムス、スコティエラまたはプロト
シフォンである方法。(2) The method according to claim 1, wherein the green algae is Chlamydomonas, Haematococcus, Anchistrodesmus, Chlorella, Chlorococcus, Cerastrum, Cursigenia, Dictyococcus, Scenedesmus, Scotiera, or Protosiphon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63012328A JP2707572B2 (en) | 1988-01-22 | 1988-01-22 | Method for producing astaxanthin-rich green algae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63012328A JP2707572B2 (en) | 1988-01-22 | 1988-01-22 | Method for producing astaxanthin-rich green algae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01187082A true JPH01187082A (en) | 1989-07-26 |
| JP2707572B2 JP2707572B2 (en) | 1998-01-28 |
Family
ID=11802241
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63012328A Expired - Fee Related JP2707572B2 (en) | 1988-01-22 | 1988-01-22 | Method for producing astaxanthin-rich green algae |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2707572B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100447940B1 (en) * | 2002-03-21 | 2004-09-10 | 학교법인 한양학원 | Development of profitable prey, green algae Coelastrum sp. |
| WO2016104487A1 (en) * | 2014-12-22 | 2016-06-30 | 国立大学法人東京大学 | Method for mass production of carotenoids |
| JP2018070569A (en) * | 2016-11-04 | 2018-05-10 | 株式会社ユーグレナ | Hepatic stellate cell activation inhibitor and food composition for inhibiting hepatic stellate cell activation |
-
1988
- 1988-01-22 JP JP63012328A patent/JP2707572B2/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100447940B1 (en) * | 2002-03-21 | 2004-09-10 | 학교법인 한양학원 | Development of profitable prey, green algae Coelastrum sp. |
| WO2016104487A1 (en) * | 2014-12-22 | 2016-06-30 | 国立大学法人東京大学 | Method for mass production of carotenoids |
| JPWO2016104487A1 (en) * | 2014-12-22 | 2017-09-28 | 国立大学法人 東京大学 | Carotenoid mass production method |
| JP2018070569A (en) * | 2016-11-04 | 2018-05-10 | 株式会社ユーグレナ | Hepatic stellate cell activation inhibitor and food composition for inhibiting hepatic stellate cell activation |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2707572B2 (en) | 1998-01-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA1333345C (en) | Pigmentation supplements for animal feed compositions | |
| Kobayashi et al. | Astaxanthin production by a green alga, Haematococcus pluvialis accompanied with morphological changes in acetate media | |
| Hemaiswarya et al. | Microalgae: a sustainable feed source for aquaculture | |
| Cysewski et al. | Industrial Production of Microalgal Cell‐Mass and Secondary Products‐Species of High Potential: Haematococcus | |
| US20040077036A1 (en) | Process to produce astaxanthin from haematococcus biomass | |
| US8173391B2 (en) | Golden yellow algae and method of producing the same | |
| JPWO2005085465A1 (en) | Method for producing astaxanthin-containing lipid | |
| CN105452442A (en) | Methods of producing algal cell cultures and biomass, lipid compounds and compositions, and related products | |
| US12058984B2 (en) | Aquaculture shrimps and methods for culturing same | |
| JPH01187082A (en) | Production of astaxanthin-rich chlorophyceae | |
| JP3665084B2 (en) | Carotenoid pigment stabilization method, carotenoid pigment stabilization composition, and feed containing carotenoid pigment stabilized by the method | |
| JP2018515117A (en) | Fermentation method for decolorizing the biomass of Chlorella Protothecoides | |
| JPH0870848A (en) | Euglena algal body highly containing vitamin e and beta-carotene and production | |
| AU2004322412B2 (en) | An economical and efficient method for mass production of spirulina | |
| Stunda-Zujeva et al. | Growing and drying Spirulina/Arthrospira for producing food and nutraceuticals: A Review | |
| JPH09327268A (en) | Feed additive for cultured fish and feed for cultured fish | |
| CN109527202B (en) | Method for preparing feed rich in docosahexaenoic acid and beta carotene and feed | |
| Santos Ribeiro et al. | Production of Chlorella protothecoides biomass, chlorophyll and carotenoids using the dairy industry by-product scotta as a substrate | |
| CN107969575A (en) | A kind of red cream crab feed containing natural pigment raw material | |
| CN118026302A (en) | Water quality improver for aquaculture and preparation method and use method thereof | |
| CN111642704A (en) | Method for inhibiting oxidation of fat and protein in sausage by using crude extract of rhodobacter sphaeroides | |
| KR20120000405A (en) | Mass propagation method of cyanobacteria | |
| JP3337745B2 (en) | Feed composition | |
| TH14556A (en) | How to color crayfish | |
| CN104839500A (en) | Feed capable of improving red fish flavor and preparation method of feed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |