JPH01160475A - Device for immobilizing microorganism - Google Patents
Device for immobilizing microorganismInfo
- Publication number
- JPH01160475A JPH01160475A JP31925187A JP31925187A JPH01160475A JP H01160475 A JPH01160475 A JP H01160475A JP 31925187 A JP31925187 A JP 31925187A JP 31925187 A JP31925187 A JP 31925187A JP H01160475 A JPH01160475 A JP H01160475A
- Authority
- JP
- Japan
- Prior art keywords
- aqueous solution
- microorganisms
- calcium salt
- polymerization catalyst
- salt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 42
- 230000003100 immobilizing effect Effects 0.000 title 1
- 239000007864 aqueous solution Substances 0.000 claims abstract description 31
- 159000000007 calcium salts Chemical class 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 239000002685 polymerization catalyst Substances 0.000 claims abstract description 27
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 16
- 238000006116 polymerization reaction Methods 0.000 claims abstract description 16
- 150000003839 salts Chemical class 0.000 claims abstract description 12
- 235000010443 alginic acid Nutrition 0.000 claims abstract description 10
- 239000000783 alginic acid Substances 0.000 claims abstract description 10
- 229960001126 alginic acid Drugs 0.000 claims abstract description 10
- 229920000615 alginic acid Polymers 0.000 claims abstract description 10
- 150000004781 alginic acids Chemical class 0.000 claims abstract description 9
- 239000000243 solution Substances 0.000 claims abstract description 5
- 229940079593 drug Drugs 0.000 claims description 10
- 239000003814 drug Substances 0.000 claims description 10
- 239000011259 mixed solution Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 abstract description 6
- 239000007921 spray Substances 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- 231100000331 toxic Toxicity 0.000 abstract description 3
- 230000002588 toxic effect Effects 0.000 abstract description 3
- 239000011575 calcium Substances 0.000 abstract 1
- 239000003054 catalyst Substances 0.000 abstract 1
- 239000000178 monomer Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000012266 salt solution Substances 0.000 description 3
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 2
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 description 2
- 238000005842 biochemical reaction Methods 0.000 description 2
- 239000000648 calcium alginate Substances 0.000 description 2
- 235000010410 calcium alginate Nutrition 0.000 description 2
- 229960002681 calcium alginate Drugs 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- JRKICGRDRMAZLK-UHFFFAOYSA-L persulfate group Chemical group S(=O)(=O)([O-])OOS(=O)(=O)[O-] JRKICGRDRMAZLK-UHFFFAOYSA-L 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- IMROMDMJAWUWLK-UHFFFAOYSA-N Ethenol Chemical compound OC=C IMROMDMJAWUWLK-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- XTXRWKRVRITETP-UHFFFAOYSA-N Vinyl acetate Chemical compound CC(=O)OC=C XTXRWKRVRITETP-UHFFFAOYSA-N 0.000 description 1
- -1 alginic acid salt Chemical class 0.000 description 1
- 229910001870 ammonium persulfate Inorganic materials 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229910001622 calcium bromide Inorganic materials 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- WGEFECGEFUFIQW-UHFFFAOYSA-L calcium dibromide Chemical compound [Ca+2].[Br-].[Br-] WGEFECGEFUFIQW-UHFFFAOYSA-L 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- AXZAYXJCENRGIM-UHFFFAOYSA-J dipotassium;tetrabromoplatinum(2-) Chemical compound [K+].[K+].[Br-].[Br-].[Br-].[Br-].[Pt+2] AXZAYXJCENRGIM-UHFFFAOYSA-J 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical class OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 229910001487 potassium perchlorate Inorganic materials 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- 159000000001 potassium salts Chemical class 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- BAZAXWOYCMUHIX-UHFFFAOYSA-M sodium perchlorate Chemical compound [Na+].[O-]Cl(=O)(=O)=O BAZAXWOYCMUHIX-UHFFFAOYSA-M 0.000 description 1
- 229910001488 sodium perchlorate Inorganic materials 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、種々の化学反応や廃水処理に有用な固定化微
生物を製造する微生物固定化装置に関する。DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a microorganism immobilization device for producing immobilized microorganisms useful for various chemical reactions and wastewater treatment.
〔従来技術及び発明が解決しようとする問題点〕従来、
重合性薬剤と微生物及びアルギン酸ナトリウムの混合液
を、カルシウム塩を混合した重合触媒水溶液中に滴下し
、水に不溶のアルギン酸カルシウムを生成させながら重
合性薬剤を重合させる装置が知られている。この装置で
は、簡単な操作で微生物を固定化できる。しかし、重合
触媒として一般に用いられる過硫酸塩は、微生物に対す
る毒性が強いので、微生物活性が著しく低下してしまう
。[Prior art and problems to be solved by the invention] Conventionally,
An apparatus is known in which a liquid mixture of a polymerizable drug, a microorganism, and sodium alginate is dropped into an aqueous solution of a polymerization catalyst mixed with a calcium salt, and the polymerizable drug is polymerized while producing water-insoluble calcium alginate. With this device, microorganisms can be immobilized with simple operations. However, persulfates commonly used as polymerization catalysts are highly toxic to microorganisms, resulting in a significant decrease in microbial activity.
従って、強度と微生物活性が共に高い固定化微生物を得
るには、比較的高濃度の重合触媒に重合性混合液の液滴
を短時間だけ接触させ、短時間内に重合を終了させる必
要がある。 ・しかしながら、従来の装置では
、カルシウム塩含有重合触媒水溶液中に混合液の液滴が
必要以上に長時間接触され、微生物の活性が低下してし
まうという欠点があった。Therefore, in order to obtain immobilized microorganisms with high strength and microbial activity, it is necessary to bring droplets of the polymerizable mixture into contact with a relatively highly concentrated polymerization catalyst for only a short period of time, and to complete the polymerization within a short period of time. . - However, the conventional apparatus has the disadvantage that the droplets of the mixed liquid are in contact with the calcium salt-containing polymerization catalyst aqueous solution for a longer time than necessary, resulting in a decrease in the activity of the microorganisms.
従って、本発明は、前記の従来技術の欠点を解消し、強
度及び微生物活性が共に高い固定化微生物を製造できる
微生物固定化装置を提供することを目的とする。Therefore, an object of the present invention is to provide a microorganism immobilization device that can eliminate the drawbacks of the prior art described above and can produce immobilized microorganisms with high strength and microbial activity.
本発明は、疎水性液層中にカルシウム塩含有重合触媒水
溶液の微細な液滴を浮遊させ、そこに微生物、重合性薬
剤及びアルギン酸水溶性塩の混合液の液滴を滴下し、上
記の微細な液滴と混合液の液滴とを接触させることによ
って活性の高い固定化微生物が得られることを見出し、
前記の問題点を解決したものである。In the present invention, fine droplets of a calcium salt-containing polymerization catalyst aqueous solution are suspended in a hydrophobic liquid layer, and droplets of a mixed solution of microorganisms, a polymerizable drug, and an alginic acid water-soluble salt are dropped therein. discovered that highly active immobilized microorganisms could be obtained by bringing liquid droplets into contact with droplets of a mixed liquid.
This solves the above problems.
すなわち、本発明の微生物固定化装置は、微生物、重合
性薬剤及びアルギン酸の水溶性塩を含む混合液の滴下装
置、カルシウム塩を混合した重合触媒水溶液の滴下装置
及び上層に疎水性液、下層にカルシウム塩水溶液を含む
重合槽から成ることを特徴とする。That is, the microorganism immobilization device of the present invention includes a dropping device for dropping a mixed solution containing a microorganism, a polymerizable drug, and a water-soluble salt of alginic acid, a dropping device for dropping a polymerization catalyst aqueous solution mixed with a calcium salt, a hydrophobic liquid in the upper layer, and a hydrophobic liquid in the lower layer. It is characterized by consisting of a polymerization tank containing an aqueous calcium salt solution.
本発明に使用する滴下装置は、通常、ノズルを有する装
置であるが、液体の小滴を滴下しうるちのであればよい
、また、本発明の固定化装置においては、重合触媒水溶
液を重合槽の上層の疎水性液層に微細な液滴として浮遊
させておくので、重合触媒水溶液の滴下装置としては、
スプレーノズルを使用するのが好ましい。The dropping device used in the present invention is usually a device having a nozzle, but any device capable of dropping small droplets of liquid may be used. Since it is suspended as fine droplets in the upper hydrophobic liquid layer, it can be used as a dropping device for aqueous polymerization catalyst solution.
Preferably, a spray nozzle is used.
本発明の固定化装置に使用する重合槽は、その一端から
カルシウム塩水溶液を流入させ、他端から生成固定化微
生物を排出させうる形式のものであれば、任意のもので
よい。この重合槽中で、下層を形成するカルシウム塩水
溶液は、通常、０．２〜５重量％水溶液、好ましくは０
．５〜２重量％水溶液として使用する。他方、重合槽の
上層をなす疎水性液としては、水と混和せず、比重が１
より小さく、微生物に対する毒性がほとんどない有機液
体、例えば植物油、パラフィン等を使用することができ
る。The polymerization tank used in the immobilization apparatus of the present invention may be of any type as long as it is of a type that allows the calcium salt aqueous solution to flow in from one end and discharge the produced immobilized microorganisms from the other end. In this polymerization tank, the calcium salt aqueous solution forming the lower layer is usually a 0.2 to 5% by weight aqueous solution, preferably a 0.2 to 5% by weight aqueous solution.
．． It is used as a 5-2% by weight aqueous solution. On the other hand, the hydrophobic liquid that forms the upper layer of the polymerization tank is immiscible with water and has a specific gravity of 1.
Organic liquids that are smaller and have little toxicity to microorganisms, such as vegetable oils, paraffin, etc., can be used.
本発明の固定化装置により固定化する微生物には、特別
に制限はなく、活性汚泥、硝化菌、メタン発酵菌、シュ
ードモナス属菌、その他、生化学反応及び化学反応に関
与する各種の細菌であってよい。The microorganisms to be immobilized by the immobilization device of the present invention are not particularly limited, and include activated sludge, nitrifying bacteria, methane-fermenting bacteria, Pseudomonas bacteria, and various other bacteria involved in biochemical and chemical reactions. It's fine.
重合性薬剤は、重合性モノマー及び溶媒、架橋剤等のモ
ノマーの重合に通常使用される物質（重合触媒を除く）
を含むものである。重合性モノマーとしては、アクリル
酸、メタクリル酸、アクリル酸エステル若しくはアクリ
ルアミド又はこれらに対応するメタクリル酸誘導体、酢
酸ビニル、ビニルアルコール等のビニルモノマーが挙ケ
ラレ、これらのモノマーの１種又は２種以上を使用する
ことができる。更に、これらの七ツマ−と共重合可能な
モノマーを重合性薬剤に包含することもできる。Polymerizable agents include polymerizable monomers, solvents, crosslinking agents, and other substances normally used for monomer polymerization (excluding polymerization catalysts).
This includes: Examples of polymerizable monomers include acrylic acid, methacrylic acid, acrylic esters, acrylamide, or their corresponding methacrylic acid derivatives, and vinyl monomers such as vinyl acetate and vinyl alcohol. can be used. Furthermore, monomers copolymerizable with these heptamers can also be included in the polymerizable drug.
混合液には、更にアルギン酸の水溶性塩が含まれる。水
溶性塩としては、ナトリウム塩、カリウム塩、アンモニ
ウム塩等が挙げられる。アルギン酸の水溶性塩は、混合
液中に０．１〜２重量％、好ましくは０．２〜０．５重
量％の濃度で含まれる。The liquid mixture further contains a water-soluble salt of alginic acid. Examples of water-soluble salts include sodium salts, potassium salts, ammonium salts, and the like. The water-soluble salt of alginic acid is contained in the mixed liquid at a concentration of 0.1 to 2% by weight, preferably 0.2 to 0.5% by weight.
重合触媒としては、過硫酸カリウム、過硫酸アンモニウ
ム等の過硫酸塩類、過塩素酸カリウム、過塩素酸ナトリ
ウム等の過塩素酸塩類、過酸化水素等が挙げられる。こ
れらは、０．００１〜５重量％、好ましくは０．１〜１
重量％水溶液として用いられる。Examples of the polymerization catalyst include persulfates such as potassium persulfate and ammonium persulfate, perchlorates such as potassium perchlorate and sodium perchlorate, and hydrogen peroxide. These are 0.001 to 5% by weight, preferably 0.1 to 1% by weight.
Used as a wt% aqueous solution.
重合触媒水溶液中には、混合液滴中のアルギン酸の水溶
性塩が疎水性液層内で不溶性塩を形成できるように、水
溶性のカルシウム塩を添加しておく。使用しうる水溶性
カルシウム塩としては、塩化カルシウム、臭化カルシウ
ム、硝酸カルシウム等が挙げられる。カルシウム塩は、
重合触媒水溶液中に０．２〜５重量％、好ましくは０．
５〜２重景％の濃度で添加する。A water-soluble calcium salt is added to the polymerization catalyst aqueous solution so that the water-soluble salt of alginic acid in the mixed droplets can form an insoluble salt in the hydrophobic liquid layer. Water-soluble calcium salts that can be used include calcium chloride, calcium bromide, calcium nitrate, and the like. Calcium salt is
0.2 to 5% by weight, preferably 0.2 to 5% by weight in the polymerization catalyst aqueous solution.
Add at a concentration of 5-2%.
次に、図面に示した本発明の実施例に基づいて本発明を
詳述する。Next, the present invention will be described in detail based on embodiments of the present invention shown in the drawings.
第１図は、本発明の一実施例を示す微生物固定化装置の
略示断面図である。FIG. 1 is a schematic cross-sectional view of a microorganism immobilization device showing one embodiment of the present invention.
第１図に示した固定化装置において、重合槽１は上層に
疎水性液２、下層にカルシウム塩水溶液３を貯えている
。カルシウム塩水溶液３は水路状の重合槽１の一端に設
けた流入管４から流入し、排出管５を設けた他端に向か
って流下する。In the immobilization apparatus shown in FIG. 1, a polymerization tank 1 stores a hydrophobic liquid 2 in the upper layer and an aqueous calcium salt solution 3 in the lower layer. The calcium salt aqueous solution 3 flows into the waterway-shaped polymerization tank 1 through an inflow pipe 4 provided at one end thereof, and flows down toward the other end provided with a discharge pipe 5.
重合槽１は、顆粒側が深くなるように底面が傾斜してい
る。また、重合を阻害する酸素を除去するため、重合槽
１の上部には、窒素ガス等の不活性ガスを導通ずるのが
好ましい。The bottom of the polymerization tank 1 is inclined so that the granule side is deeper. Further, in order to remove oxygen that inhibits polymerization, it is preferable to conduct an inert gas such as nitrogen gas through the upper part of the polymerization tank 1.
重合触媒槽６は、カルシウム塩を混合した重合触媒水溶
液７を収容しており、スプレーノズル８は、ポンプ９で
圧送された重合触媒水溶液７を霧化して微細液滴１０を
作り、該液滴を重合槽１の上流端付近の疎水性液２中に
落下させる。微細液滴１０は、疎水性液層内のカルシウ
ム塩水溶液３との界面に達し、浮遊しながら流下し、界
面を満たしていく。The polymerization catalyst tank 6 contains a polymerization catalyst aqueous solution 7 mixed with calcium salt, and the spray nozzle 8 atomizes the polymerization catalyst aqueous solution 7 pumped by a pump 9 to form fine droplets 10. is dropped into the hydrophobic liquid 2 near the upstream end of the polymerization tank 1. The fine droplets 10 reach the interface with the calcium salt aqueous solution 3 in the hydrophobic liquid layer, flow down while floating, and fill the interface.
混合液槽１１は、微生物、重合性薬剤及びアルギン酸水
溶性塩の混合液１２を収容しており、微生物と薬剤が均
一に混合するように攪拌機を用いて攪拌される。滴下装
置１４は、比較的細いノズル１５を備えており、ポンプ
１６により圧送された混合液１２を微細液滴１０が浮遊
している疎水性液２中に滴下し、混合液滴１７を作る。The mixed liquid tank 11 contains a mixed liquid 12 of microorganisms, a polymerizable drug, and a water-soluble alginic acid salt, and is stirred using a stirrer so that the microorganisms and the drug are uniformly mixed. The dropping device 14 is equipped with a relatively narrow nozzle 15, and drops the mixed liquid 12 pumped by the pump 16 into the hydrophobic liquid 2 in which the fine droplets 10 are suspended, thereby forming mixed droplets 17.
混合液滴１７の直径は、通常、１〜５鶴程度のものであ
る。The diameter of the mixed droplet 17 is usually about 1 to 5 cranes.
混合液滴１７は、疎水性液層内のカルシウム塩水溶液と
の界面に達し、ここに浮遊するカルシウム塩を混合した
重合触媒水溶液の微細液滴１０と接触し、まず、アルギ
ン酸の水溶性塩が水に不溶性のカルシウム塩に変わり、
微生物及び重合性薬剤を包囲するアルギン酸カルシウム
の皮膜が形成され、更に、内部の重合性薬剤が重合固化
して混合液滴１７とほぼ同じ大きさの固定化微生物１８
になる。このとき、混合液滴１７と微細液滴１０の接触
効率を高め、また、混合液滴１７同士の合体を防ぐため
、微細液滴１０の直径は、固定化微生物１８の粒径より
小さい、好ましくはｌｆ１以内であることが望ましい。The mixed droplets 17 reach the interface with the calcium salt aqueous solution in the hydrophobic liquid layer and come into contact with the fine droplets 10 of the polymerization catalyst aqueous solution mixed with the calcium salt floating there. Converts to water-insoluble calcium salts,
A film of calcium alginate surrounding the microorganisms and the polymerizable drug is formed, and the polymerizable drug inside is further polymerized and solidified to form an immobilized microorganism 18 of approximately the same size as the mixed droplet 17.
become. At this time, in order to increase the contact efficiency between the mixed droplets 17 and the fine droplets 10 and to prevent the mixed droplets 17 from coalescing, the diameter of the fine droplets 10 is preferably smaller than the particle size of the immobilized microorganisms 18. is preferably within lf1.
微細液滴１０の直径は、スプレーノズル１８の孔径とポ
ンプ９の圧力を変えることにより調節することができる
。The diameter of the fine droplets 10 can be adjusted by changing the pore size of the spray nozzle 18 and the pressure of the pump 9.
更に、滴下された混合液滴１７は、疎水性液層内のカル
シウム塩水溶液３との界面を浮遊、流下し、重合して固
定化微生物１８に変わりながら、数秒から長くとも数十
秒以内に自然に下層のカルシウム塩水溶液３中に重力沈
降する。そのため、固定化微生物１８が必要以上に重合
触媒水溶液７と接触するのを防ぎ、微生物の活性低下を
防止することができる。Furthermore, the dropped mixed droplet 17 floats and flows down the interface with the calcium salt aqueous solution 3 in the hydrophobic liquid layer, polymerizes, and transforms into the immobilized microorganism 18 within a few seconds to several tens of seconds at the most. It naturally settles by gravity into the lower calcium salt aqueous solution 3. Therefore, it is possible to prevent the immobilized microorganisms 18 from coming into contact with the polymerization catalyst aqueous solution 7 more than necessary, and to prevent a decrease in the activity of the microorganisms.
界面に広がった微細液滴１０も、やがてはカルシウム塩
水溶液３に溶は込み、そこの重合触媒濃度を高めていく
が、カルシウム塩水溶液槽１９に収容された新鮮なカル
シウム塩水溶液３をポンプ２０を用いて流入管４から供
給することにより重合触媒を微生物活性に影響しない程
度のごく低濃度に抑えることができる。The fine droplets 10 that have spread on the interface will eventually dissolve into the calcium salt aqueous solution 3 and increase the polymerization catalyst concentration there. By supplying the polymerization catalyst from the inlet pipe 4 using a polymerization catalyst, the concentration of the polymerization catalyst can be suppressed to a very low concentration that does not affect microbial activity.
カルシウム塩水溶液３中に沈降した固定化微生物１８は
、排出管５から水溶液と共に流出し、網状のベルトコン
ベア２１で回収され、貯留槽２２に貯留される。ベルト
コンベア２１を通過したカルシウム塩水溶液３は、循環
水槽２３に貯留され、一部は廃液としてオーバーフロー
し、残りはポンプ２４を用いて流入管４に循環される。The immobilized microorganisms 18 that have settled in the calcium salt aqueous solution 3 flow out from the discharge pipe 5 together with the aqueous solution, are collected by a mesh belt conveyor 21, and stored in a storage tank 22. The aqueous calcium salt solution 3 that has passed through the belt conveyor 21 is stored in a circulating water tank 23, a portion of which overflows as waste liquid, and the remainder is circulated to the inflow pipe 4 using the pump 24.
本発明の固定化装置によれば、微生物に対して毒性の高
い重合触媒と微生物が必要以上に接触することなく、微
生物を固定化することができるので、活性の著しく高い
固定化微生物を得ることができる。According to the immobilization device of the present invention, microorganisms can be immobilized without unnecessary contact between the microorganisms and the polymerization catalyst, which is highly toxic to microorganisms, so that immobilized microorganisms with extremely high activity can be obtained. Can be done.
本発明の固定化装置により製造される固定化微生物は、
廃水処理や微生物が関与する様々な生化学反応及び化学
反応に有効に利用される。The immobilized microorganism produced by the immobilization device of the present invention is
It is effectively used in wastewater treatment and various biochemical and chemical reactions involving microorganisms.
第１図は、本発明の一実施態様を示す微生物固定化装置
の略示断面図である。
符号の説明
１・・・重合槽、２・・・疎水性液、３・・・カルシウ
ム塩水溶液、６・・・重合触媒槽、８・・・スプレーノ
ズル、１１・・・混合液槽、１４・・・滴下装置、１５
・・・ノズルFIG. 1 is a schematic cross-sectional view of a microorganism immobilization device showing one embodiment of the present invention. Description of symbols 1... Polymerization tank, 2... Hydrophobic liquid, 3... Calcium salt aqueous solution, 6... Polymerization catalyst tank, 8... Spray nozzle, 11... Mixed liquid tank, 14 ...Dripping device, 15
···nozzle
Claims (2)
含む混合液の滴下装置、カルシウム塩を混合した重合触
媒水溶液の滴下装置及び上層に疎水性液、下層にカルシ
ウム塩水溶液を含む重合槽から成ることを特徴とする微
生物固定化装置。(1) From a dropping device for a mixed solution containing microorganisms, a polymerizable drug, and a water-soluble salt of alginic acid, a dropping device for a polymerization catalyst aqueous solution mixed with a calcium salt, and a polymerization tank containing a hydrophobic liquid in the upper layer and a calcium salt aqueous solution in the lower layer. A microorganism immobilization device characterized by:
れる液滴の直径が固定化微生物の粒径より小さくなるよ
うに設定した特許請求の範囲第１項記載の固定化装置。(2) The immobilization device according to claim 1, wherein the dropping device for the aqueous polymerization catalyst solution is set so that the diameter of the droplets dropped from the device is smaller than the particle size of the immobilized microorganism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP31925187A JPH01160475A (en) | 1987-12-17 | 1987-12-17 | Device for immobilizing microorganism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP31925187A JPH01160475A (en) | 1987-12-17 | 1987-12-17 | Device for immobilizing microorganism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01160475A true JPH01160475A (en) | 1989-06-23 |
| JPH0528593B2 JPH0528593B2 (en) | 1993-04-26 |
Family
ID=18108105
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP31925187A Granted JPH01160475A (en) | 1987-12-17 | 1987-12-17 | Device for immobilizing microorganism |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01160475A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8871249B2 (en) | 2008-02-27 | 2014-10-28 | Hisamitso Pharmaceutical Co., Inc. | Medicated patch |
-
1987
- 1987-12-17 JP JP31925187A patent/JPH01160475A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8871249B2 (en) | 2008-02-27 | 2014-10-28 | Hisamitso Pharmaceutical Co., Inc. | Medicated patch |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0528593B2 (en) | 1993-04-26 |
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