JPH01155995A - Improved type activated sludge treatment for pectin-containing waste water - Google Patents

Improved type activated sludge treatment for pectin-containing waste water

Info

Publication number
JPH01155995A
JPH01155995A JP62314793A JP31479387A JPH01155995A JP H01155995 A JPH01155995 A JP H01155995A JP 62314793 A JP62314793 A JP 62314793A JP 31479387 A JP31479387 A JP 31479387A JP H01155995 A JPH01155995 A JP H01155995A
Authority
JP
Japan
Prior art keywords
pectin
activated sludge
waste water
cod
sludge treatment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP62314793A
Other languages
Japanese (ja)
Inventor
Isao Akamatsu
赤松 勲
Takamasa Kubo
久保 隆昌
Hiroyuki Tanabe
田辺 寛之
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP62314793A priority Critical patent/JPH01155995A/en
Publication of JPH01155995A publication Critical patent/JPH01155995A/en
Pending legal-status Critical Current

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Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage

Landscapes

  • Activated Sludge Processes (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

PURPOSE:To convert the pectin in pectin-contg. waste water with which an activated sludge treatment is difficult to low molecules and to lower the COD concn. in the waste water simultaneously by using flocculatable pectin decomposing bacteria groups. CONSTITUTION:The bacteria having large pectin decomposing power are selected from the pectin decomposing bacteria belonging to filamentous fungus and are planted to the medium of a pectin substrate. Aerating-settling-releasing of the supernatant-adding of the medium are then repeated to generate the flocculated and settled pectin decomposing bacteria groups. After the pectin in the waste water of orange cans is converted to the low molecules by these pectin decomposing bacteria groups, the pectin is completely decomposed by the activated sludge treatment. As a result, the adsorption or intrusion of the high-polymer pectin into the activated sludge by failure in the decomposition thereof and the inclusion of such sludge into a sludge tank are prevented. The degradation in the BOD decomposing activity of the sludge is thus prevented and the concn. of the COD in the waste water of the orange cans is sufficiently lowered.

Description

【発明の詳細な説明】 〔産業上の利用分野〕 本発明は活性汚泥処理の困難なペクチン含有廃水の新規
な微生物処理方法に関する。詳しく言えば、ペクチン質
を凝集性のペクチン分解菌群を用いて低分子化した後、
活性汚泥処理によって完全分解することを特徴とするみ
かん缶詰廃水等のペクチン含有廃水の処理方法に関する
ものである。
DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a novel microbial treatment method for pectin-containing wastewater, which is difficult to treat with activated sludge. Specifically, after pectin is reduced to a low molecular weight using aggregating pectin-degrading bacteria,
The present invention relates to a method for treating pectin-containing wastewater, such as wastewater from canned mandarin oranges, which is completely decomposed by activated sludge treatment.

〔従来の技術〕[Conventional technology]

みかん缶詰廃水の処理に直接には活性汚泥処理が困難で
あったことから、メタン発酵処理を行った後、活性汚泥
処理する方式がとられている。メタン発酵が嫌気性であ
ることから、活性汚泥処理において、多量の空気を要す
ること及び処理施設費が多大である等の欠点があり、中
小企業では採用しにくい方法であった。そのような理由
から中小企業の缶詰工場では、みかん缶詰加工を断念し
ているところが多くあり、従来の処理法は不完全な処理
法である。
Since activated sludge treatment was difficult to directly treat wastewater from canned mandarin oranges, a method has been adopted in which activated sludge treatment is performed after methane fermentation treatment. Since methane fermentation is anaerobic, activated sludge treatment has drawbacks such as requiring a large amount of air and requiring a large amount of processing facility costs, making it difficult for small and medium-sized enterprises to adopt this method. For this reason, many small and medium-sized canning factories have given up on canning mandarin oranges, and conventional processing methods are incomplete.

〔発明が解決し、ようとする問題点〕[Problems that the invention attempts to solve]

従来の活性汚泥処理によってみかん缶詰廃水を処理する
場合、みかん缶詰廃水中のモノマーの中性糖類は分解さ
れるけれども、高分子のペクチンが分解されないで活性
汚泥に吸着されたリ、混入して沈澱槽に入るため、はじ
めの5日間程度は処理が可能であるが、以後は処理が不
可能になった。これは返送汚泥にペクチンが多く混入し
、汚泥のBOD分解活性が低下するためである。
When treating mandarin orange canned wastewater using conventional activated sludge treatment, although the monomer neutral sugars in the mandarin orange canned wastewater are decomposed, the polymeric pectin is not decomposed and is adsorbed to the activated sludge, mixed in and precipitated. Because it is placed in a tank, treatment is possible for the first five days, but treatment is no longer possible after that. This is because a large amount of pectin is mixed into the returned sludge, reducing the BOD decomposition activity of the sludge.

この問題点を解決するためには、ペクチンを低分子化及
び分解し、しかも凝集・沈澱性であって、沈澱槽におい
て容易に沈澱し、処理水との分離が可能なペクチン分解
菌群を見出すことが重要な課題となる。
In order to solve this problem, we need to find a group of pectin-degrading bacteria that can reduce the molecular weight of pectin and decompose it, as well as flocculate and settle, allowing it to easily settle in a settling tank and be separated from treated water. This is an important issue.

凝集・沈澱性のペクチン分解菌群の創製法について具体
的に記述する。
We will specifically describe the method for creating a group of flocculating and precipitating pectin-degrading bacteria.

一般には糸状菌は活性汚泥処理において、BOD成分を
除去するけれどもバルキングの原因になることから、防
除することが行われている。
Although filamentous fungi are generally used to remove BOD components during activated sludge treatment, they cause bulking, so they are controlled.

本発明ではペクチンを分解する糸状菌を逆に利用しよう
とするものである。すなわちペクチンを分解する糸状菌
の中からペクチン分解活性の大きなものを選抜し、本菌
群をみかん缶詰廃水を成分とする培養基を入れた坂ロフ
ラスコ中で振とう培養した。これを次にビー力を用いて
の散気管による通気培養に切り換え14日間培養して後
、静置し、菌体を沈澱させ、沈澱した菌体のみ回収した
。次にこの菌体にみかん缶詰廃水を加え同様の通気培養
を行った。この操作を6回以上繰り返した時、沈降速度
が早くなり、静置培養したこの菌体の顕微鏡観察の結果
、この菌体は糸状菌が主体であるが3種類以上の微生物
の集合体であることが分かった。
The present invention attempts to utilize filamentous fungi that degrade pectin. That is, from among the filamentous fungi that degrade pectin, those with high pectin-degrading activity were selected, and this group of bacteria was cultured with shaking in a Sakaro flask containing a culture medium containing mandarin orange canning wastewater. This was then switched to aerated culture using an aeration tube using bee force, and after culturing for 14 days, the culture was allowed to stand to precipitate the microbial cells, and only the precipitated microbial cells were collected. Next, wastewater from canned mandarin oranges was added to the bacterial cells, and the same aeration culture was performed. When this operation was repeated six times or more, the sedimentation rate increased, and as a result of microscopic observation of the statically cultured bacteria, it was found that the bacteria were mainly filamentous fungi, but were an aggregate of three or more types of microorganisms. That's what I found out.

上述のように糸状菌は一般にバルキングの原因になるが
、この凝集・沈澱性のペクチン分解菌群は全くその傾向
がなく、沈降速度も大きく、4時間以内の静置によりほ
とんどの菌体が沈殿することが分かった。それは、寒天
平面培地上では糸状菌の構造が糸状部と中間及び先端に
玉状物の胞子嚢のある糸状菌として観察されるが、本国
は不完全菌であるので曝気状態で培養すると糸状部の隔
壁の構造が弱く、こわれて糸状菌にはならないが酵素分
泌性などの生理活性は低下しないという特徴を有するこ
とが明らかになった。またペクチン分解菌群によって前
処理した上澄液を、次に通常の活性汚泥処理法によって
浄化した場合、処理水の清澄性も良いという特徴があっ
た。
As mentioned above, filamentous fungi generally cause bulking, but this group of flocculating/sedimentating pectin-degrading bacteria does not have that tendency at all, and has a high sedimentation rate, so most of the fungi will settle within 4 hours. I found out that it does. On an agar flat medium, the structure of the filamentous fungus is observed as a filamentous fungus with a filamentous part and sporangia of balls in the middle and at the tip, but since it is a deficient fungus in its home country, when cultured in an aerated state, the filamentous structure is observed as a filamentous fungus. It has been revealed that the structure of the septum is weak, and although it does not break down and become a filamentous fungus, its physiological activities such as enzyme secretion do not decrease. Furthermore, when the supernatant liquid pretreated with the pectin-degrading bacteria group was then purified by a normal activated sludge treatment method, the treated water was characterized by good clarity.

みかん缶詰廃水(COD約40001)Ellm)を本
菌群を用いて分解した場合のCODの低下を第1図に、
ペクチン分解菌群の沈降曲線を第2図に示す。また本菌
群を用いたみかん缶詰廃水の低分子化処理及び活性汚泥
処理を実施例によって具体的に説明する。
Figure 1 shows the decrease in COD when mandarin orange canning wastewater (COD approx. 40001) was decomposed using this bacterial group.
Figure 2 shows the sedimentation curve of the pectin-degrading bacteria group. In addition, low molecular weight treatment and activated sludge treatment of canned mandarin orange wastewater using this bacterial group will be specifically explained using examples.

実施例1 糸状菌に属するペクチン分解菌の中からペクチン分解力
の大きなものを選択し、これをペクチン基質の培地に植
菌し、曝気−沈殿一上清液の放流−培地添加を繰り返し
、凝集・沈殿のペクチン分解菌群を創製した。本ペクチ
ン分解菌群の沈降速度は第2図に示した様に沈降初期に
おいて大きく、はぼ4時間の沈降時間で良いことから、
廃水処理への適用は十分可能であることが分かった。
Example 1 Among the pectin-degrading bacteria belonging to filamentous fungi, those with a large pectin-degrading ability were selected, and they were inoculated into a pectin-substrate medium, and the process of aeration, precipitation, discharging the supernatant, and addition of the medium was repeated to cause flocculation.・We created a group of bacteria that degrade pectin in the sediment. As shown in Figure 2, the sedimentation rate of this pectin-degrading bacteria group is high at the early stage of sedimentation, and the sedimentation time is only about 4 hours.
It was found that application to wastewater treatment is fully possible.

実施例2 みかん缶詰工程のアルカリ処理廃水8丈と酸処理廃水8
Lからなるみかん缶詰廃水(COD3200ppm)を
苛性シータによってpH4,5とし、これに2.7gの
尿素と1.1gのリン11!2水素1カリウムを加え、
上述の凝集・沈殿性のペクチン分解菌群を加えて曝気し
た。ペクチンの分解と共にpHが上昇するので硫酸によ
ってl)HをpH8になったときDH7に降下させた。
Example 2 8 pieces of alkali-treated wastewater and 8 pieces of acid-treated wastewater from the mandarin orange canning process
Mandarin orange canning wastewater (COD 3200 ppm) consisting of L was adjusted to pH 4.5 using caustic theta, and 2.7 g of urea and 1.1 g of phosphorus 11!2 hydrogen monopotassium were added.
The above-mentioned flocculating/sedimentating pectin-degrading bacteria group was added and aerated. Since the pH increases with the decomposition of pectin, l) H was lowered to DH7 when the pH reached 8 using sulfuric acid.

−2日間のバッチ式処理における空間負荷は1 、29
 Kg −COD / at’・日であり、CODの除
去速度は1.0,2h−COD/m1日であった。この
場合のCOD除去率は79%であり、残存するペクチン
は2〜3邑体に低分子化されていた。 ′ 実施例3 実施例2と同様のみかん缶詰廃水(COD3800pp
m)に同量の尿素とリン酸2水素1カリウムを加え、p
H5,0とし、これに凝集・沈殿性のペクチン分解菌群
を加えて曝気し、28後1)H8,8になったものを硫
酸でpH7,0とし、さらに3日曝気を継続した。この
場合はpH管理が実施例2に比べて粗調整であルノテ、
CODの除去速alfi0.64Kg−COD / m
’・日と低いが、第1図に見られるように1)H制御を
しない場合よりCOD除去速度がはるかに大きいことが
分かった。
- Space load for 2 days of batch processing is 1,29
Kg -COD/at'·day, and the COD removal rate was 1.0.2 h-COD/m1 day. In this case, the COD removal rate was 79%, and the remaining pectin was reduced to 2 to 3 molecules. 'Example 3 Mandarin canned wastewater similar to Example 2 (COD 3800pp
Add the same amount of urea and monopotassium dihydrogen phosphate to m), and add p
The pH was adjusted to 5.0, and a group of flocculating and precipitating pectin-degrading bacteria was added thereto, followed by aeration.After 28 hours, the pH became 8.8 (1).The pH was adjusted to 7.0 with sulfuric acid, and aeration was continued for an additional 3 days. In this case, the pH control is roughly adjusted compared to Example 2.
COD removal rate alfi0.64Kg-COD/m
Although the COD removal rate was as low as 1.5 days, as shown in Figure 1, it was found that 1) the COD removal rate was much higher than in the case without H control.

実施例4 実施例1によって得た分解及び低分子化された中間処理
水をバッチ式活性汚泥処理によって浄化処理した結果、
COD 400 ppmの原水が8時間の曝気処理によ
ってCOD 200 ppmとなった。みかん缶詰工場
の洗浄工程水が実施例1の原水の3倍以上あることから
、水沫によって十分に排水の排出基準は守られることが
明らかになった。
Example 4 As a result of purifying the decomposed and low molecular weight intermediate treated water obtained in Example 1 by batch activated sludge treatment,
Raw water with a COD of 400 ppm became COD 200 ppm after 8 hours of aeration treatment. Since the amount of water used in the washing process at the tangerine cannery was more than three times that of the raw water in Example 1, it became clear that the water droplets were sufficient to comply with the wastewater discharge standards.

実施例5 実施例1によって得た中間処理水を曝気槽容積9j、の
連続標準活性汚泥処理によってCOD除去を行った結果
、CODの容積負荷0.9に9−COD/V・日の場合
、処理水の残存CODは198 DI)mであり、CO
D除去率62.3%であった。洗浄工程水がこの原水の
3倍以上あることから排水の排出基準は十分守られうる
Example 5 As a result of removing COD from the intermediate treated water obtained in Example 1 by continuous standard activated sludge treatment in an aeration tank volume of 9j, when the volumetric load of COD was 0.9 and 9-COD/V day, The residual COD of the treated water is 198 DI)m, and the COD
The D removal rate was 62.3%. Since the cleaning process water is more than three times the raw water, the wastewater discharge standards can be fully complied with.

実施例6 都市下水処理の活性汚泥菌2容に対し、本発明の凝集・
沈殿性ペクチン分解菌を1容の割合に混合し、これをみ
かん缶詰廃水に馴養した。
Example 6 Two volumes of activated sludge bacteria from urban sewage treatment were treated with the flocculation and
Precipitating pectin-degrading bacteria were mixed at a ratio of 1 volume, and the mixture was acclimatized to mandarin orange canning wastewater.

バッチ法によりこの種菌に対し、みかん缶詰廃水と上述
の窒素及びリンを加え、pH6,5〜pH8の範囲で曝
気処理した結果、COD1500ppmのものが20時
間で00015000mとなり、COD除去率90%が
達成された。
Using the batch method, this inoculum was treated with canned mandarin wastewater, nitrogen and phosphorus as mentioned above, and aerated in the pH range of 6.5 to pH 8. As a result, the COD of 1,500 ppm became 0,001,5,000 m in 20 hours, achieving a COD removal rate of 90%. It was done.

これはペクチンの低分子化とCODの除去を同時に行う
方法である。
This is a method that simultaneously reduces the molecular weight of pectin and removes COD.

【図面の簡単な説明】[Brief explanation of the drawing]

第1図は凝集・沈殿性のペクチン分解菌によってペクチ
ン含有廃水を処理する場合pHを制御した場合としない
場合の処理日数の差を示したものである。第2図は凝集
・沈殿性ペクチン分解菌群の沈降速度を表わす沈降曲線
を示したものである。 特許出願人  工業技術院長 飯塚幸三処理日数 (日
 )
FIG. 1 shows the difference in the number of treatment days when pectin-containing wastewater is treated with flocculating and precipitating pectin-degrading bacteria, with and without pH control. FIG. 2 shows a sedimentation curve representing the sedimentation rate of the flocculating/sedimentating pectin-degrading bacteria group. Patent applicant: Director of the Agency of Industrial Science and Technology Kozo Iizuka Processing days (days)

Claims (3)

【特許請求の範囲】[Claims] (1)ペクチン質を凝集性ペクチン分解菌群で低分子化
した後、活性汚泥処理することを特徴とするペクチン含
有廃水の処理方法。
(1) A method for treating pectin-containing wastewater, which comprises reducing the molecular weight of pectin with a group of flocculating pectin-degrading bacteria and then treating it with activated sludge.
(2)ペクチン質廃水を凝集性ペクチン分解菌群を含ん
だ活性汚泥を用いて処理することを特徴とするペクチン
含有廃水の処理方法。
(2) A method for treating pectin-containing wastewater, which comprises treating pectin-containing wastewater using activated sludge containing flocculating pectin-degrading bacteria.
(3)凝集性ペクチン分解菌群がペクチン基質で馴養し
て得られた凝集性の糸状菌であることを特徴とする特許
請求範囲第1項及び第2項記載のペクチン含有廃水処理
方法。
(3) The pectin-containing wastewater treatment method according to claims 1 and 2, wherein the flocculating pectin-degrading bacteria group is a flocculating filamentous fungus obtained by acclimatization with a pectin substrate.
JP62314793A 1987-12-11 1987-12-11 Improved type activated sludge treatment for pectin-containing waste water Pending JPH01155995A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62314793A JPH01155995A (en) 1987-12-11 1987-12-11 Improved type activated sludge treatment for pectin-containing waste water

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62314793A JPH01155995A (en) 1987-12-11 1987-12-11 Improved type activated sludge treatment for pectin-containing waste water

Publications (1)

Publication Number Publication Date
JPH01155995A true JPH01155995A (en) 1989-06-19

Family

ID=18057663

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62314793A Pending JPH01155995A (en) 1987-12-11 1987-12-11 Improved type activated sludge treatment for pectin-containing waste water

Country Status (1)

Country Link
JP (1) JPH01155995A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010094607A (en) * 2008-10-16 2010-04-30 Kochi Univ Method of cleaning waste water containing pectin
CN103204591A (en) * 2013-04-15 2013-07-17 杭州浙大易泰环境科技有限公司 Microbiological treatment process for pectin wastewater

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS53117255A (en) * 1977-03-22 1978-10-13 Kuraray Co Ltd Method of treating waste water containing pectic substances
JPS6028893A (en) * 1983-07-26 1985-02-14 Tax Adm Agency Treatment of waste water

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS53117255A (en) * 1977-03-22 1978-10-13 Kuraray Co Ltd Method of treating waste water containing pectic substances
JPS6028893A (en) * 1983-07-26 1985-02-14 Tax Adm Agency Treatment of waste water

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010094607A (en) * 2008-10-16 2010-04-30 Kochi Univ Method of cleaning waste water containing pectin
CN103204591A (en) * 2013-04-15 2013-07-17 杭州浙大易泰环境科技有限公司 Microbiological treatment process for pectin wastewater
CN103204591B (en) * 2013-04-15 2014-10-15 杭州浙大易泰环境科技有限公司 Microbiological treatment process for pectin wastewater

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