JPH01128933A - Hyaluronidase inhibitor - Google Patents

Hyaluronidase inhibitor

Info

Publication number
JPH01128933A
JPH01128933A JP62286187A JP28618787A JPH01128933A JP H01128933 A JPH01128933 A JP H01128933A JP 62286187 A JP62286187 A JP 62286187A JP 28618787 A JP28618787 A JP 28618787A JP H01128933 A JPH01128933 A JP H01128933A
Authority
JP
Japan
Prior art keywords
hyaluronidase
extract
inhibitor
hyaluronitase
active ingredient
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP62286187A
Other languages
Japanese (ja)
Inventor
Fusako Shimada
島田 房子
Yoshio Asaga
良雄 浅賀
Yasuharu Kawajiri
康晴 川尻
Minoru Fukuda
實 福田
Masato Hayashi
正人 林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shiseido Co Ltd
Original Assignee
Shiseido Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shiseido Co Ltd filed Critical Shiseido Co Ltd
Priority to JP62286187A priority Critical patent/JPH01128933A/en
Publication of JPH01128933A publication Critical patent/JPH01128933A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74Biological properties of particular ingredients
    • A61K2800/78Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782Enzyme inhibitors; Enzyme antagonists

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Dermatology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cosmetics (AREA)

Abstract

PURPOSE:To obtain a hyaluronidase inhibitor, containing a crude drug extract essence as an active ingredient and having high safety without any irritancy or hormonal side effects on the skin. CONSTITUTION:A hyaluronidase inhibitor obtained by containing a crude drug selected from the group consisting of Paeonia Radix, Coptidis Rhizoma, Phellodendri Cortex, Mountan Cortex, Geranium Herba, Thea sinensis L., Sophorae Radix, Clematis brevicaudata DC., Lamium album L. var. barbatum Franch. et Savat., Salvia officinalis L., Juniperus communis L., Hamamelis virginiana L. and birch or a solvent extract essence (any of extract solution, diluted solution or concentrated extract or dried powder thereof) of a plant selected from the afore-mentioned group as an active ingredient. The above- mentioned inhibitor is useful for prophylaxis and prevention of diseases caused by hyaluronidase of suppuration, inflammation and I-type allergy.

Description

【発明の詳細な説明】 〔産業上の利用分野〕 本発明は、新規なヒアルロニターゼ阻害剤に関する。さ
らに詳しくは、化膿、炎症、■型アレルギーのヒアルロ
ニターゼに起因する疾患を予防、防止するための新規か
つ安全性の高いヒアルロニターゼ阻害剤に関する。
DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a novel hyaluronidase inhibitor. More specifically, the present invention relates to a novel and highly safe hyaluronidase inhibitor for preventing and preventing diseases caused by hyaluronidase, such as suppuration, inflammation, and type II allergies.

〔従来の技術〕[Conventional technology]

従来、人体におけるヒアルロニターゼは皮膚表層に常在
するプロピオニバクテリウム アクネス(Propio
nibacterium acnes )や皮膚上の一
過性の菌であるスタフィロコッカス アウレウス(St
aphyrococcus aureus )などがそ
の活性を有していることが知られている(Appl、 
Microbiol。
Conventionally, hyaluronidase in the human body is produced by Propionibacterium acnes (Propio
nibacterium acnes) and Staphylococcus aureus (St.
aphyrococcus aureus) and others are known to have this activity (Appl,
Microbiol.

gy  q、1434〜1436.196B)。スタフ
ィロコッカスアウレウスは化膿する際の原因菌として知
られているが、それが起こる際に菌がヒアルロニターゼ
を放出して皮膚組織のヒアルロニターゼを分解し増殖を
用意にしているという報告がある(Brit。
gy q, 1434-1436.196B). Staphylococcus aureus is known as the causative bacterium when suppuration occurs, and there is a report that when this occurs, the bacterium releases hyaluronidase and decomposes the hyaluronidase in the skin tissue, preparing it for proliferation (Brit).

J、Exp、Path、26.124.1945)。J, Exp, Path, 26.124.1945).

一方菌体由来以外の人体のヒアルロニターゼは生体内の
さまざまな組織中に、通常不活性な状態で存在するが、
Na+、K+、Ca+、Mg2+などの生体内金属イオ
ンおよびスペルミンや、スペルミジンなどの生体内有機
塩基により活性化される。
On the other hand, human hyaluronitase other than bacterial cell-derived hyaluronidase normally exists in an inactive state in various tissues within the body.
It is activated by in-vivo metal ions such as Na+, K+, Ca+, Mg2+, and in-vivo organic bases such as spermine and spermidine.

人体におけるヒアルロニターゼの生理学的意義は未だ不
明な点が多いが、毛細血管の透過性を光道させる起炎酵
素であること(J、Dental Re5t、33゜1
14.1958)などから、炎症と関係深い酵素である
ことがいわれている(Arch、 Pathol、、8
5,272゜1968)。またクロモグリク酸ナトリウ
ムなどのI型抗アレルギー剤が本酵素を阻害することな
どから、■型アレルギーにおける本酵素の関与が示唆さ
れている(Immunopharm、、2.139.1
980)。
The physiological significance of hyaluronidase in the human body is still unclear, but it is an inflammatory enzyme that increases the permeability of capillaries (J, Dental Re5t, 33゜1
14.1958), it is said to be an enzyme closely related to inflammation (Arch, Pathol, 8).
5,272°1968). In addition, since type I antiallergic agents such as sodium cromoglycate inhibit this enzyme, the involvement of this enzyme in type II allergies has been suggested (Immunopharm, 2.139.1
980).

以上のように化膿時の悪化、炎症、I型アレルギーにヒ
アルロニターゼの関与が示唆されておりヒアルロニター
ゼを阻害すればこれらの疾患が軽減すると考えられる。
As described above, it has been suggested that hyaluronidase is involved in exacerbation during suppuration, inflammation, and type I allergy, and it is thought that inhibiting hyaluronidase will alleviate these diseases.

現在までにヒアルロニターゼを阻害する抗炎症剤(イン
ドメタシン、アスピリン、メフェナム酸など)や、抗ア
レルギー剤(クロモグリク酸ナトリウム、トラニラスト
、トラキサノックス、バイカレインホスフエイト、ケト
チフェン、アゼラスチン、シアニダノール、オキサトラ
イドなど)が報告されている(Physiol、 Re
v、 27.436.1947゜炎症と抗炎症療法、医
歯薬出版、7.411.1982)。
To date, there are anti-inflammatory drugs that inhibit hyaluronidase (indomethacin, aspirin, mefenamic acid, etc.) and anti-allergic drugs (sodium cromoglycate, tranilast, traxanox, baicalein phosphate, ketotifen, azelastine, cyanidanol, oxatride, etc.). has been reported (Physiol, Re
v, 27.436.1947゜Inflammation and anti-inflammatory therapy, Ishiyaku Publishing, 7.411.1982).

生薬については、甘草成分のりクイリチゲニンやイソリ
クイリチゲニン、黄募成分のパイカリン、バイカレイン
などに強いヒアルロニターゼ阻害が認められている。
Regarding herbal medicines, strong hyaluronitase inhibition has been observed in licorice ingredients such as Noriquiritigenin and Isoliquiritigenin, as well as picalin and baicalein, which are licorice ingredients.

、しかしこれらの物質はほかの配合成分との関係からヒ
アルロニターゼ阻害効果を発揮できなかったり、局所適
用における安全性有効性の点で必ずしも満足しえない。
However, these substances may not be able to exhibit hyaluronidase inhibitory effects due to their relationship with other ingredients, or may not always be satisfactory in terms of safety and effectiveness when applied topically.

〔発明が解決しようとする問題点〕[Problem that the invention seeks to solve]

本発明は、現存するヒアルロニターゼ阻害剤の満足でき
ない点を考慮し、安全性が高く真にヒアルロニターゼ阻
害効果に優れた薬剤を得るべく鋭意研究を重ねた結果、
現在地の用途に使用されている数多くの生薬抽出エキス
にヒアルロニターゼ■害作用があるという全く新規な事
実を見出しこの知見にもとづいて本発明を完成するに至
った。
The present invention was developed in consideration of the unsatisfactory points of existing hyaluronitase inhibitors, and as a result of intensive research to obtain a drug that is highly safe and truly has an excellent hyaluronitase inhibitory effect.
We have discovered a completely new fact that many extracts of herbal medicines currently in use have harmful effects on hyaluronitase, and based on this knowledge we have completed the present invention.

〔問題を解決するための手段〕[Means to solve the problem]

すなわち本発明は、シャクヤク、オオレン、オオバク、
ボタンピ、ゲンノショウコ、茶、クジン、シボタンツル
、オドリコソウ、サルビア、西洋ネズ、ハマメリスおよ
びバーチからなる群の1種または2種以上から選ばれる
生薬、または上記群の1種または2種以上から選ばれた
植物の溶媒抽出エキスを有効成分とするヒアルロニター
ゼ阻害剤を提供するものである。
That is, the present invention can be applied to peonies, orensis, orientalis,
A herbal medicine selected from one or more of the group consisting of Botanical herb, blackberry, tea, Japanese vine, white vine, dead nettle, salvia, juniper, Hamamelis, and birch, or a plant selected from one or more of the above group. The present invention provides a hyaluronidase inhibitor containing a solvent extracted extract of .

以下本発明について詳しく説明する。The present invention will be explained in detail below.

本発明における生薬とは植物体を乾燥し、粉砕したもの
であり、溶媒抽出エキスとは、特に明記しない限り次の
方法によって得られる上記植物またはその生薬抽出液、
その希釈液、その濃縮エキスあるいはその乾燥床を意味
するものとする。
In the present invention, the herbal medicine is a dried and crushed plant, and the solvent-extracted extract means the above-mentioned plant or herbal medicine extract obtained by the following method, unless otherwise specified.
It shall mean its diluted solution, its concentrated extract or its drying bed.

溶媒抽出エキスは、上記植物または生薬乾燥床を、水も
しくは有機溶媒(石油エーテル、シクロヘキサン、四塩
化炭素、トルエン、ベンゼン、ジクロルメタン、クロロ
ホルム、エーテル、酢酸エチル、ブタノール、アセトン
、n−プロパツール、エタノール、メタノール、ピリジ
ン、ポリエチレングリコール、プロピレングリコール)
あるいはそれらを一定の比率で混合した溶媒、たとえば
、水性アルコールを用い、通常3℃〜70℃で抽出処理
して得られる。以上の生薬、溶媒抽出は、そのまま、ま
たは希釈あるいは濃縮し、もしくは凍結乾燥した後、粉
末またはペースト状に調製し所望により適宜製剤化しヒ
アルロニターゼ阻害剤として用いることができる。
Solvent extraction extracts are obtained by extracting the above plants or herbal medicines from a dry bed using water or organic solvents (petroleum ether, cyclohexane, carbon tetrachloride, toluene, benzene, dichloromethane, chloroform, ether, ethyl acetate, butanol, acetone, n-propanol, ethanol). , methanol, pyridine, polyethylene glycol, propylene glycol)
Alternatively, it can be obtained by extraction using a solvent in which they are mixed at a certain ratio, such as aqueous alcohol, usually at 3°C to 70°C. The above herbal medicines and solvent extraction can be used as hyaluronidase inhibitors as they are, or after being diluted, concentrated, or lyophilized, prepared into a powder or paste form, and formulated into an appropriate formulation as desired.

〔発明の効果〕l1−tA・“〔費穂otり本発明のヒ
アルロニターゼ阻害剤は以下に説明する実施例から分か
るように、ヒアルロニターゼを強力かつ有効に抑制する
。さらに皮膚に対する刺激やホルモン様副作用を全く与
えないので皮膚に対して非常に安全である。
[Effects of the Invention] The hyaluronidase inhibitor of the present invention strongly and effectively inhibits hyaluronidase, as can be seen from the examples described below.Furthermore, it does not cause irritation to the skin or hormone-like side effects. It is extremely safe for the skin as it does not give any irritation to the skin.

(実施例1) (1)  ヒアルロニターゼの調製 ヒアルロニターゼ(牛畢丸由来シグマ社製)を最終濃度
が340 NFunit / mlとなるように0.1
M酢酸緩衝液PH3,5調製した。
(Example 1) (1) Preparation of hyaluronitase Hyaluronitase (derived from Ushibimaru, manufactured by Sigma) was added to a final concentration of 0.1 to give a final concentration of 340 NFunit/ml.
M acetate buffer PH3,5 was prepared.

(2)溶媒抽出エキス試料の調製 乾燥し粉砕した生薬1〜10gに10倍量の水、メタノ
ールまたはこれらの混液を加え、振盪抽出するかまたは
、加熱還流下に抽出した後上漬と沈渣に分離した。上清
を減圧下に濃縮乾固して生薬抽出エキスを得、必要に応
じてこれを水またはメタノールで希釈して生薬抽出エキ
ス試料とした。
(2) Preparation of solvent-extracted extract sample Add 10 times the amount of water, methanol, or a mixture thereof to 1 to 10 g of dried and ground herbal medicine, and extract with shaking or extract under heating and reflux, and then soak in the supernatant and precipitate. separated. The supernatant was concentrated to dryness under reduced pressure to obtain a crude drug extract, which was diluted with water or methanol as necessary to prepare a crude drug extract sample.

(3)  ヒアルロニターゼ活性測定法ヒアルロニター
ゼ活性測定は、モルガンーエルソン(Morgan−E
lson)法を応用する方法に従って行った(ibid
、、242,437.1967 )。
(3) Hyaluronitase activity measurement method Hyaluronitase activity measurement is carried out by Morgan-Ellson (Morgan-E
It was carried out according to the method of applying the method (ibid
, 242, 437.1967).

′酵素の緩衝溶液0.1mlに、酵素の活性化剤の緩衝
液0.2mlずつを加え、37℃で20分間放置し酵素
を活性化した。次に(2)溶媒抽出エキス試料の調製で
調製した溶媒抽出エキス試料の緩衝液あるいは炭酸ナト
リウム中和水溶液0.2mlずつを加え、37℃で20
分間放置した。対象として用いる酵素溶液には、試料の
緩衝溶液あるいは炭酸ナトリウム中和水溶液0.2ml
のかわりに、緩衝液あるいは水0.2mlずつを加えた
。その後ヒアルロン酸カリウム緩衝液0.5mlを加え
、37℃で40分間反応させた。
'To 0.1 ml of the enzyme buffer solution, 0.2 ml of the enzyme activator buffer was added, and the mixture was left at 37° C. for 20 minutes to activate the enzyme. Next, add 0.2 ml each of the buffer solution or sodium carbonate neutralized aqueous solution of the solvent extracted extract sample prepared in (2) Preparation of the solvent extracted extract sample, and
Leave it for a minute. The enzyme solution used as a target contains 0.2 ml of the sample buffer solution or sodium carbonate neutralized aqueous solution.
Instead, 0.2 ml each of buffer solution or water was added. Thereafter, 0.5 ml of potassium hyaluronate buffer was added and reacted at 37°C for 40 minutes.

0.4N水酸化ナトリウム0.2n+1を加えて反応を
停止した後、モルガンーエルフン法により発色させ58
5mmにおける吸光度を測定した。
After stopping the reaction by adding 0.2n+1 of 0.4N sodium hydroxide, color was developed by the Morgan-Elfn method.
Absorbance at 5 mm was measured.

Oモルガンーエルフン法 各反応液にホウ酸カリ溶液を加え、水溶中で3分間加熱
した後、流水中で冷却し、P−ジメチルアミノベンズア
ルデヒド試薬6.0LIllを加えた。
O. Morgan-Elfn method Potassium borate solution was added to each reaction solution, heated in water for 3 minutes, cooled in running water, and 6.0 LIll of P-dimethylaminobenzaldehyde reagent was added.

直ちに振盪した後、37℃で20分間発色させ585m
mにおける吸光度を測定した。
After shaking immediately, the color was developed for 20 minutes at 37°C.
The absorbance at m was measured.

(ホウ酸カリ溶液:  0,8Mホウ酸水溶液100+
alに水酸化カリウム2.24gを加え溶解した。
(Potassium boric acid solution: 0.8M boric acid aqueous solution 100+
2.24 g of potassium hydroxide was added to al and dissolved.

P−ジメチルアミノベンズアルデヒド:P−ジメチルア
ミノベンズアルデヒド10gを酢酸87 、5m lお
よびION塩酸12.5mlに溶解した。本試薬は通常
冷蔵保存しておき、用時酢酸で10倍希釈した。
P-dimethylaminobenzaldehyde: 10 g of P-dimethylaminobenzaldehyde was dissolved in 5 ml of acetic acid 87 and 12.5 ml of ION hydrochloric acid. This reagent is usually stored refrigerated and diluted 10 times with acetic acid before use.

阻害率は、式(1)により算出した。The inhibition rate was calculated using formula (1).

compound48/ 80 (シグマ社製)の最終
濃度が、それぞれ2.5n+M、0.15Mおよび0.
1mg/mlとなるように調製した緩衝溶液を用いた。
The final concentrations of compound 48/80 (manufactured by Sigma) were 2.5n+M, 0.15M and 0.5n+M, respectively.
A buffer solution prepared to have a concentration of 1 mg/ml was used.

その結果を表−1に示した。The results are shown in Table-1.

(比較例1) (1)  ヒアルロニターゼの調製 実施例1の(1)ヒアルロニターゼの調製と同様に調製
した。
(Comparative Example 1) (1) Preparation of hyaluronitase It was prepared in the same manner as (1) Preparation of hyaluronitase in Example 1.

(2)  ヒアルロニターゼ阻害剤の調製ヒアルロニタ
ーゼ阻害効果のある薬剤として知られているトラニラス
ト、クロルモノグリフ酸ナトリウム、シアニダノールの
試料を調製した。
(2) Preparation of hyaluronitase inhibitors Samples of tranilast, sodium chlormonoglyphate, and cyanidanol, which are known as drugs having a hyaluronitase inhibitory effect, were prepared.

(3)  ヒアルロニターゼ活性の測定法実施例1 、
(3)ヒアルロニターゼ活性の測定法に準じて操作した
(3) Example 1 of the method for measuring hyaluronidase activity,
(3) The procedure was performed according to the method for measuring hyaluronidase activity.

その結果を表−1に示した。The results are shown in Table-1.

表−1各種生薬抽出エキスのヒアルロニターゼ阻害効果
とヒアルロニターゼ表−1から明らかなように本発明に
係るヒアルロニターゼ阻害剤は、生薬抽出エキス試料が
反応系中に0.001〜0.1%存在する場合、20%
〜85%のヒアルロニターゼ阻害作用を示すことがわか
る。
Table 1: Hyaluronitase inhibitory effect of various herbal medicine extracts and hyaluronidase As is clear from Table 1, the hyaluronidase inhibitor according to the present invention is effective when the crude medicine extract sample is present in the reaction system at 0.001 to 0.1%. ,20%
It can be seen that it exhibits ~85% hyaluronidase inhibitory effect.

さらにヒアルロニターゼ阻害剤として知られているトラ
ニラスト、クロルモノグリフ酸ナトリウム、シアニダノ
ールは、反応系中に0.1%存在しても、クロルモノグ
リフ酸トリウム以外は50%以下のヒアルロニターゼ阻
害作用しかないことがわかった。
Furthermore, even if tranilast, sodium chlormonoglyphate, and cyanidanol, which are known as hyaluronitase inhibitors, are present in the reaction system at 0.1%, they have a hyaluronitase inhibitory effect of less than 50%, except for thorium chlormonoglynate. I understand.

特許出願人  株式会社 資生堂Patent applicant: Shiseido Co., Ltd.

Claims (1)

【特許請求の範囲】[Claims] シャクヤク、オオレン、オオバク、ボタンピ、ゲンノシ
ョウコ、茶、クジン、シボタンツル、オドリコソウ、サ
ルビア、西洋ネズ、ハマメリス、およびバーチからなる
群の1種または2種以上から選ばれる生薬、または上記
群の一種または2種以上から選ばれた植物の溶媒抽出エ
キスを有効成分とするヒアルロニターゼ阻害剤
Herbal medicines selected from one or more of the group consisting of peonies, oriental laurel, giant tabara, botanpi, genus shoko, tea, kujin, white vine, dead nettle, salvia, juniper, hamamelis, and birch, or one or two of the above groups. A hyaluronitase inhibitor whose active ingredient is a solvent-extracted extract of a plant selected from the above.
JP62286187A 1987-11-12 1987-11-12 Hyaluronidase inhibitor Pending JPH01128933A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP62286187A JPH01128933A (en) 1987-11-12 1987-11-12 Hyaluronidase inhibitor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP62286187A JPH01128933A (en) 1987-11-12 1987-11-12 Hyaluronidase inhibitor

Publications (1)

Publication Number Publication Date
JPH01128933A true JPH01128933A (en) 1989-05-22

Family

ID=17701074

Family Applications (1)

Application Number Title Priority Date Filing Date
JP62286187A Pending JPH01128933A (en) 1987-11-12 1987-11-12 Hyaluronidase inhibitor

Country Status (1)

Country Link
JP (1) JPH01128933A (en)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0211520A (en) * 1988-06-29 1990-01-16 Momotani Jiyuntenkan:Kk Hyaluronidase inactivation agent
JPH0377829A (en) * 1989-08-18 1991-04-03 Kiyuukiyuu Yakuhin Kogyo Kk Crude drug plaster preparation
JPH0616530A (en) * 1992-07-03 1994-01-25 Mikimoto Pharmaceut Co Ltd Cosmetic
JPH0987189A (en) * 1995-09-19 1997-03-31 Ichimaru Pharcos Co Ltd Antiallergic agent containing isodon japonicus hara, paeonia suffruticosa andrews, perilla frutescens britton var. acuta kudo, and/or arunica montana linne
JP2000297044A (en) * 1999-04-13 2000-10-24 Sunstar Inc Transdermal patch for external use for skin
US6193977B1 (en) 1999-03-18 2001-02-27 Medvill Co., Ltd. Pharmaceutical composition comprising an aqueous extract of a mixture of anemarrhena rhizoma and phellodedron bark for analgesic and anti-inflammation
JP2003012489A (en) * 2001-07-02 2003-01-15 Naris Cosmetics Co Ltd Hyaluronidase activity inhibitor and moisture-retaining cosmetic
JP2003238432A (en) * 2002-02-15 2003-08-27 Fancl Corp Hyaluronic acid acuumulation-accelerating agent
US6630176B2 (en) * 2000-03-07 2003-10-07 Mount Sinai School Of Medicine Of New York University Herbal remedies for treating allergies and asthma
JP2004002433A (en) * 2003-06-02 2004-01-08 Pola Chem Ind Inc Skin external preparation for alpha-msh suppression
KR100720670B1 (en) * 2006-02-22 2007-05-21 경희대학교 산학협력단 The composition comprising extracts of phellodendri cortex and coptis chinensis for treatment of type iv allergy and an inflammation
KR100720671B1 (en) * 2006-02-23 2007-05-21 경희대학교 산학협력단 The composition comprising extracts of gentiana scabra, citrus unshiu marcor and coptis chinensis for treatment of type iv allergy and an inflammation
JP2009067804A (en) * 2008-10-30 2009-04-02 Pola Chem Ind Inc SKIN EXTERNAL PREPARATION FOR WHITENING RELATING TO alpha-MSH
JP2010260808A (en) * 2009-05-01 2010-11-18 Ikeda Mohando:Kk Oral medicinal composition for improving skin condition
US8101211B2 (en) 2000-12-15 2012-01-24 Kabushiki Kaisha Yakult Honsha Compositions for retarding skin aging
JP2023145053A (en) * 2022-03-28 2023-10-11 株式会社ナリス化粧品 Hyaluronidase inhibitor

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS55127309A (en) * 1979-03-23 1980-10-02 Kishiyouhin Kagaku Kaihou Kenkyusho:Kk Cosmetic
JPS5838209A (en) * 1981-08-29 1983-03-05 Sunstar Inc Tannin-containing composition for skin for external application
JPS5973509A (en) * 1982-10-18 1984-04-25 Osaka Chem Lab Cosmetic composition containing ogon
JPS59216810A (en) * 1983-05-24 1984-12-06 Osaka Chem Lab Cosmetic composition containing catechin compound
JPS60146829A (en) * 1984-01-05 1985-08-02 Rooto Seiyaku Kk Testosterone 5alpha-reductase inhibitor
JPS6150922A (en) * 1984-08-20 1986-03-13 Ichimaru Fuarukosu Kk External drug for skin containing extracted component of "oubaku"
JPS62207224A (en) * 1986-03-08 1987-09-11 Nippon Haipotsukusu:Kk Side effect reducing preparation for antitumor agent
JPS6450877A (en) * 1987-08-20 1989-02-27 Ichimaru Pharcos Inc Oxygen radical catching and removing agent

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS55127309A (en) * 1979-03-23 1980-10-02 Kishiyouhin Kagaku Kaihou Kenkyusho:Kk Cosmetic
JPS5838209A (en) * 1981-08-29 1983-03-05 Sunstar Inc Tannin-containing composition for skin for external application
JPS5973509A (en) * 1982-10-18 1984-04-25 Osaka Chem Lab Cosmetic composition containing ogon
JPS59216810A (en) * 1983-05-24 1984-12-06 Osaka Chem Lab Cosmetic composition containing catechin compound
JPS60146829A (en) * 1984-01-05 1985-08-02 Rooto Seiyaku Kk Testosterone 5alpha-reductase inhibitor
JPS6150922A (en) * 1984-08-20 1986-03-13 Ichimaru Fuarukosu Kk External drug for skin containing extracted component of "oubaku"
JPS62207224A (en) * 1986-03-08 1987-09-11 Nippon Haipotsukusu:Kk Side effect reducing preparation for antitumor agent
JPS6450877A (en) * 1987-08-20 1989-02-27 Ichimaru Pharcos Inc Oxygen radical catching and removing agent

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0211520A (en) * 1988-06-29 1990-01-16 Momotani Jiyuntenkan:Kk Hyaluronidase inactivation agent
JPH0377829A (en) * 1989-08-18 1991-04-03 Kiyuukiyuu Yakuhin Kogyo Kk Crude drug plaster preparation
JPH0616530A (en) * 1992-07-03 1994-01-25 Mikimoto Pharmaceut Co Ltd Cosmetic
JPH0987189A (en) * 1995-09-19 1997-03-31 Ichimaru Pharcos Co Ltd Antiallergic agent containing isodon japonicus hara, paeonia suffruticosa andrews, perilla frutescens britton var. acuta kudo, and/or arunica montana linne
US6193977B1 (en) 1999-03-18 2001-02-27 Medvill Co., Ltd. Pharmaceutical composition comprising an aqueous extract of a mixture of anemarrhena rhizoma and phellodedron bark for analgesic and anti-inflammation
JP2000297044A (en) * 1999-04-13 2000-10-24 Sunstar Inc Transdermal patch for external use for skin
US6630176B2 (en) * 2000-03-07 2003-10-07 Mount Sinai School Of Medicine Of New York University Herbal remedies for treating allergies and asthma
US8101211B2 (en) 2000-12-15 2012-01-24 Kabushiki Kaisha Yakult Honsha Compositions for retarding skin aging
JP2003012489A (en) * 2001-07-02 2003-01-15 Naris Cosmetics Co Ltd Hyaluronidase activity inhibitor and moisture-retaining cosmetic
JP2003238432A (en) * 2002-02-15 2003-08-27 Fancl Corp Hyaluronic acid acuumulation-accelerating agent
JP4542300B2 (en) * 2002-02-15 2010-09-08 株式会社ファンケル Hyaluronic acid accumulation promoter
JP2004002433A (en) * 2003-06-02 2004-01-08 Pola Chem Ind Inc Skin external preparation for alpha-msh suppression
JP4574959B2 (en) * 2003-06-02 2010-11-04 ポーラ化成工業株式会社 External skin preparation for suppressing α-MSH
KR100720670B1 (en) * 2006-02-22 2007-05-21 경희대학교 산학협력단 The composition comprising extracts of phellodendri cortex and coptis chinensis for treatment of type iv allergy and an inflammation
KR100720671B1 (en) * 2006-02-23 2007-05-21 경희대학교 산학협력단 The composition comprising extracts of gentiana scabra, citrus unshiu marcor and coptis chinensis for treatment of type iv allergy and an inflammation
JP2009067804A (en) * 2008-10-30 2009-04-02 Pola Chem Ind Inc SKIN EXTERNAL PREPARATION FOR WHITENING RELATING TO alpha-MSH
JP2010260808A (en) * 2009-05-01 2010-11-18 Ikeda Mohando:Kk Oral medicinal composition for improving skin condition
JP2023145053A (en) * 2022-03-28 2023-10-11 株式会社ナリス化粧品 Hyaluronidase inhibitor

Similar Documents

Publication Publication Date Title
JPH01128933A (en) Hyaluronidase inhibitor
US9138451B2 (en) Plant extract hydrolysates and antibacterial product containing the same
US10967030B2 (en) Traditional Chinese medicine composition for treating psoriasis and method for preparing the same
CN102293724B (en) Cosmetic composition containing Chinese herbal compounds
HU229230B1 (en) Medical composition for the treatment of herpes simplex virus and other infectious diseases
WO2008140200A1 (en) External compositions for the skin
JPS60156618A (en) Cosmetic or medicinal composition based on plant extract
CN102579525A (en) Ilex rotunda thunb extract and preparation method and application thereof
CN102258637A (en) Callicarpa nudiflora extract, and preparation method and application thereof
CN102258746B (en) Ointment for treating surface of burns and scalds
Kamburoğlu et al. Analgesic effect of Anatolian propolis in mice
Manosroi et al. Potent hypoglycemic effect of Nigerian anti-diabetic medicinal plants
Cheesman et al. An assessment of the growth inhibition profiles of Hamamelis virginiana L. extracts against Streptococcus and Staphylococcus spp.
Nand et al. In vitro and in vivo assessment of polyherbal topical gel formulation for the treatment of acne vulgaris
CN101040957B (en) Medicine for treating skin diseases such as eczema and dermatitis and the method for preparing the same
Aina et al. In vitro investigations into the antioxidant and anti-Inflammatory potentials of the fractions and ethanolic extract of Cyclosorus afer (christ.) Ching, stalks
JPH0687731A (en) Cosmetic
JP2599152B2 (en) Agent for prevention and treatment of pasturellosis in pigs
JPH01128934A (en) Protease inhibitor
JP3292869B2 (en) Hyaluronidase inhibitor
JPH01172340A (en) Preventive and remedy for staphylococcal disease of chicken
CN111388523A (en) Preparation for treating acne and preparation method thereof
JP3487619B2 (en) 5α-reductase inhibitor
CN111358851A (en) Gel for treating pet skin diseases and preparation method thereof
Soni et al. Formulation, development and evaluation of polyherbal gel for topical infection