JPH01115499A - Method for removing dimethyl amines - Google Patents

Abstract

PURPOSE:To make dimethyl amine harmless by bringing a liquid contg. dimethyl amine and the salt thereof and the bacterial soma of the bacterium which can metabolize and decompose the dimethyl amine of Methylobacillus genus, etc., or the salt thereof or the treated matter of the bacterial soma of the bacterium into contact with each other. CONSTITUTION:The liquid contg. the dimethyl amine and/or the dimethyl amine salt and the bacteria soma of the bacterium which belongs to any of the Methylobacillus geneus, Protomonas genus, Methylofargas geneus, Pseudomonas geneus, Azotobacter geneus, and Mycobacterium geneus and can metabolize and decompose the dimethyl amine and/or the dimethyl amine salt and/or the treated matter of the bacterial soma of the bacterium are brought into contact with each other. The dimethyl amine and/or the dimethyl amine salt in the liquid is thereby decomposed and removed. The soln. contg. the dimethyl amine is adequately prepd. into a nearly neutral soln. at the time of the bacterial soma treatment thereof.

Description

[Detailed Description of the Invention] C. Industrial Application Field] The present invention is directed to the use of dimethyl The present invention relates to a method for removing amines, and more particularly to a method for removing dimethylamines from a liquid containing dimethylamines using bacteria.

[Prior Art and Problems to be Solved by the Invention] Dimethylamine is contained in wastewater from dimethylamine manufacturing plants, and is also used in various products such as tetramethylammonium hydroxide, dimethylformamide, and trimethylamine. As a decomposition product, it is contained in wastewater from chemical factories. However, these effluents containing dimethylamine are harmful and must be treated to render them harmless before being discharged from the viewpoint of environmental hygiene.However, conventional activated sludge treatment makes such effluents harmless. could not be converted into

Dimethylamines are stable and difficult to decompose substances, but if we can find microorganisms that can efficiently assimilate or decompose these substances, we can use these microorganisms to efficiently remove wastewater containing dimethylamines. It becomes possible to make it harmless.

[Means and effects for solving the problem] The present inventors,
As a result of extensive exploration of the natural world, we discovered microorganisms that can actively assimilate or strongly decompose dimethylamines, and completed the present invention using this microorganism.

That is, the present invention provides a liquid containing dimethylamine and/or a dimethylamine salt, and Methylobacillus, Protomonas, Methylophaga 5, Pseudomonas,
Belongs to either the genus Arthrohactor or Mycobacterium and assimilates dimethylamine and/or dimethylamine salts.

Removal of dimethylamines, characterized by decomposing and removing dimethylamine and/or dimethylamine salts in the liquid by contacting with decomposable bacterial cells and/or a bacterial cell-treated product of the bacteria. It's a method.

The bacteria used in the present invention belong to any of the genera Methylobacillus, Protomonas, Methylophaga, Pseudomonas, Arthrobacter and Mycobacterium, and have the ability to assimilate and decompose dimethylamines. There are no particular restrictions as long as it is a bacterial strain.

A representative species of bacteria belonging to the genus Methylobacillus is, for example, Methylobacillus glycogenes. This genus and species were identified in the 8TCC by Yordy and Weaver in 1977.
29475 (・JCM 2850・NCIB 1137
5) was established for one strain (J, R, Yordy
& T, L, Weaver: “Int, J, 5yst
, Bacteriol,” 27.247-2.55
(1977)), but in 1986, Aburakami and Komagata corrected the genus Methylobacillus and Methylobacillus glycogenes (Teizi Ur.
akami & Kazuo Komagata: “In
t, J, 5yst, Bacteriol, 36+ 5
02-511 (1986)), several bacterial species belonging to the genera Achromobacter, Methyromonas, Methylomonas, Protaminobacter, and Pseudomonas are now included in Methylobacillus glycogenes.

Furthermore, the classification of oil and Komagata ["J, Gen.

8pp1. Microbio1. ”32.317-34
1 (1986) and J.

Gen, 8pp I, M 1crob io I
, "3yoa' 135-165 (1986))
According to , several members of each of the genera Aeromonas, Corynebacterium, Methylomonas, and Pseudomonas have been identified as Methylobacillus glycogenes.

Representative examples of strains that assimilate and decompose dimethylamine include Achromobacter methanomonas ATCC21452,
Same as TCC21591, Methanomonas methanolica NR
RL B-5458, Protaminobacter candidus ΔTCC 21372, Protaminobacter thiaminophagus ATCC 21371, ATCC 21957゜Pseudomonas methylotropha NCTB 10508゜Same NCIB 10514. Same NCIB 10515
．． Methylomonas metatsukataralesrica, Microtechnical Research Institute No. 4037.

Methylomonas Methanofractolica
No. 42, Methylomonas sp.
No. 661, FEIKER No. 2662, Aeromonas methanocola FEI No. 2809, and Aeromonas methanophilum FEI No. 2808.

Pseudomonas inaudita Microtechnical Research Institute No. 169
2, Fiber Technology Research Institute No. 1693, and Fiber Technology Research Institute No. 1694.

Currently, all of these strains are considered to belong to the genus Methylobacillus according to the above-mentioned classification of oil and Komagata.

Protomonas extroquens is a representative example of bacteria belonging to the genus Protomonas. This genus and species were established in 1984 by Aburagami and Komagata (
Teizi Urakami & Kazuo Kom
agata.

“Int, J, 5yst, Bacteriol,” 3
4.188~20! (1984)), several species of the genera Mycoplana, Protaminobacter, Pseudomonas and Thiobacillus are associated with this Protomonas.
It is considered to belong to Extroquence.

Representative strains that assimilate and decompose dimethylamines include, for example, Protomonas extroquens NC.
IB 10598. Same NCIB 10599.
Same NCIB10600, Same NCIB 10601. Same N
Cl310602. NCIB10605 and NC
There are r310611, etc.

In addition, there is Protomonas extroquens TH-15 (Feikoken Bibori No. 9466), which the present inventors isolated from soil.

The mycological properties of Protomonas extroquens TH-15 are shown below.

1. Cultured at 30°C for 3 days in a methanol-containing liquid medium and a methanol-containing agar medium, respectively.

■ Straight rod Width 0.9~1.2 JIrR Length 1, 5~
4 jpm■ Population, unicellular or bicellular.

■ Has motility. It has polar flagella.

■ Presence or absence of spores Not produced.

■ Durham staining Negative.

■ Anti-acidity Negative.

■ Extracellular adhesive is not produced.

2. Growth status in each of the following media (culture at 30°C for 3 days unless otherwise specified) Morphology and properties of colonies on broth agar plate: External shape - circular, size - 2 to 3 mm.

Ridges - hemispherical, structure - homogeneous, surface - smooth.

Margin: Smooth with golden edge, Single color: Red or pink, Transparency: Opaque, Hardness: Grainy.

■ Methanol-containing agar plate medium Same as in broth agar plates.

■ Broth agar slant medium Grows vigorously and uniformly along the inoculation line.

Colony morphology and properties: Ridges - Moderate 1 Surface - Smooth 9 Edges - Smooth.

Color - red or pink, transparency - opaque.

Hardness - buttery.

■ Methanol-containing agar slant medium Same as in broth agar slants.

■ Broth liquid medium Grows weakly throughout. There is precipitation. Does not form bacterial rings.

■ Peptone water liquid medium Grows weakly throughout. There is precipitation. Does not form bacterial rings.

■ Methanol-containing liquid medium Grows all over. There is precipitation. Does not form bacterial rings.

■ Meat juice agar puncture culture Grows uniformly in the shape of small papillae. On the surface of the medium, the diameter is 2-4
Grows in a circular shape on the trunk.

■ Puncture culture on agar containing methanol Grows uniformly in the shape of small papillae. On the surface of the medium, the diameter is 2-4
Grows in a circular shape.

[Phase] Gravy gelatin high-rise culture Culture at 20°C for 10 days.

Bacterial growth is observed, but gelatin is not liquefied.

■ Ridmus milk Cultured at 30″C for 4 weeks.

Although it is allowed to grow in the country, it does not produce alkali.

3. Physiological properties ■ Reduction of nitrate Weakly reduces nitrate to nitrite.

■ MR test Negative.

■ VP test Negative.

■ Indole production Negative.

■ Generation of hydrogen sulfide Negative.

■ Starch hydrolysis Negative.

■ Use of citric acid (combined Koser medium and Chris Tensen medium) Do not use.

■ Use of nitrogen sources Ammonium salts, nitrates, urea and peptone are used as nitrogen sources.

■ Production of pigment Produces a red water-insoluble pigment in the bacterial cells.

[Phase] Urease positive.

■ Kafrase positive.

@Generation of ammonia　　　　　　　　　　Generate.

■ Growth range p] Grows in the range of 15 to 9. The range of p116 to 8 is preferable.

Grows at temperatures between 5 and 35"C. Preferably between 25 and 32"C.

■　Attitude towards oxygen　　　Aerobic.

■ Oxidase Negative.

■ O-F test (Hyu Lifeson FlughL
(by Eifson method) Decomposes sugar oxidatively, but not fermentatively.
(Left below) ■ Assimilation of sugars, production of acids, and production of gas Assimilation: Assimilates and decomposes.

■ Assimilation of carbon sources other than sugars □ [Phase] Salt resistance 3wtχNaC1-containing medium does not grow.

[Phase] Vitamin requirement Absolutely does not require vitamins.

0 Denitrification reaction Negative.

@CC (guanine + cytosine) content 67.3mo1% 0 Main bacterial fatty acid composition Monounsaturated fatty acids C+a:+ ■ Major hydroxy acids 3-hydroxy acid C34.

@ Quinone type Ubiquinone QIO [phase]
Separation source soil.

Among its mycological properties, this strain has polar flagella, is a Durham-negative methanol-assimilating bacterium that forms red colonies, and has a CC content of 67.3 mol! , monounsaturated fatty acid CI8. 1 is the main bacterial fatty acid composition, and 3-hydroxy acid CI4+. is the main hydroxy acid, and the quinone type is ubiquinone Q.

Therefore, oil-based and Komagata classification (TeiziU
rakami & Kazuo Komagata,”
rnt, J, 5yst, Bacteriol, ``En, 1
88-201 (1984)), and is identified as a microorganism belonging to Protomonas extroquens.

In addition, in 1985, Bousfield
1eld) and Green, this bacterial species is Methylobacterium Methylobacterium.
Methylobacterium ex is a bacterial species belonging to the genus m.
It is proposed that it should be called “I
ntj, 5yst, Bacteriol,” 35+
209 (1985)).

As described above, Protomonas extroquens is also called Methylobacterium extroquens, and its taxonomic name is currently in flux and has not been finalized. I'll just call it Extroquence.

Of the strains described in the paper by Urakami and Komagata, Protomonas extroquens TK 0
001 (=NCIB 9399. Pseudomonas extroquence), same TK 0003 (=ATC
C8457 = DS Gate 1340 = I-Shin 1081 = IFO
3708=NCIB 2879. Protaminobacter Louver, same TK 0004 (JTCC1471B=D
SM 1338=NCIB 9133= r'eel
& Quale AM 1. Bacterial strains such as Pseudomonas sp. actively assimilate dimethylamines1.
Can be disassembled.

A representative example of a bacterial strain succumbing to the genus Methylophaga is Methylophaga salicica NCMB 2162. Same ATCC
33145, and Methylophaga sp. Microtechnical Research Institute No. 3169 and Methylophaga Sp. Microtechnology Research Institute No. 3622. Methylophaga sp. 3169
Pseudomonas sarummetanolica and the same number are 3622, respectively.
No. 9 and Methylomonas Sarasica Phys. No. 36
It was deposited as No. 22, but now it is used as Urakami and Komagata part R ("J, Gen, Appl, Microbio+
, “32, 317-341 (1986) and “
J, Gen, Appl, Micro-biol,”33
．． 135-165 (1987)], Methi o7
It is said to be a bacterial strain belonging to the genus Faga.

A representative example of a strain belonging to the genus Pseudomonas is Pseudomonas aminovorans Tl-3
467), and DM-81 (Feikoken Bibori No. 9498).

These strains were isolated from soil by the present inventors.

The mycological properties of these strains are shown below.

1. At 30°C for both the liquid broth medium and agar agar medium.
The cells were cultured for 1 day.

■ Direct rod width 0.9~1.2- length 1.5~4μ■
Can be clustered, unicellular or bicellular.

■ Has motility. It has polar flagella.

■ Presence or absence of spores Not produced.

Q Gram staining Negative.

■ Anti-acidity Negative.

■ Extracellular adhesive is not produced.

2. Growth status on each of the following media (culture at 30°C for 3 days unless otherwise specified) Morphology and properties of colonies on broth agar medium: External shape - round, size - 2 to 3 mm.

Ridges - hemispherical, structure - homogeneous, surface - smooth.

Edges: Smooth with gold edges, 3 colors: white or yellowish white, transparency.
Opaque, hardness - buttery.

■ Monomethylamine-containing agar plates Same as in broth agar plates.

■ Broth agar slant medium Grows uniformly and vigorously along the tangent '4 rfi line.

Colony morphology and properties: Raised - Moderate 5 Surface - Smooth 2 Margin - Smooth.

Color - white or yellow-white, transparency - opaque.

Hardness: Hardness.

■ Monomethylamine-containing agar slants Same as in broth agar slants.

■ Grows throughout the broth liquid medium. There is precipitation. Does not form a closed ring. Tl1
-3 exhibits brown color.

■ Peptone water liquid medium Grows weakly throughout. There is precipitation. Does not form a closed ring.

■ Monomethylamine-containing liquid medium Grows all over. There is precipitation. Does not form a closed ring.

■ Meat juice agar puncture culture Grows uniformly in the shape of small papillae. On the surface of the medium, the diameter is 2-4
Grows in a circular shape about mm in diameter.

■ Monomethylamine-containing agar puncture culture Grows uniformly in the shape of small papillae. On the surface of the medium, it grows in a circular shape with a diameter of 2 to 4 inches.

[Phase] Gravy gelatin high-rise culture Culture at 20°C for 10 days.

Bacterial growth is observed, but gelatin is not liquefied.

■ Ridmus milk Cultured at 30°C for 4 weeks.

Although bacterial growth is observed, alkali is not produced.

3. Physiological properties ■ Reduction of nitrate Reducing nitrate to nitrite.

■ MR test Negative.

■ VP test Negative.

■ Indole production Negative.

■ Generation of hydrogen sulfide Negative.

■ Starch hydrolysis Negative.

■ Use of citric acid (combined Koser medium and Chris Tensen medium) Do not use.

■ Use of nitrogen sources Ammonium salts, nitrates, urea and peptone are used as nitrogen sources.

■ Pigment generation　　　　　　　　No generation.

[Phase] Urease positive.

■ Catalase positive.

@Generation of ammonia　　　　　　　　　　Generate.

■Growth range: Grows in the range of ρ116 to 8. pl+ 6.5-7.
A range of 5 is preferred.

Grows at temperatures between 5 and 35°C. 10-32°C is preferred.

■　Attitude towards oxygen　　　Aerobic.

■ Oxidase Negative.

■ O-F test (Hyu Lifeson II u g
hLeifson method) Decomposes sugar oxidatively, but not fermentatively.
(Left below) o jIH Cervical assimilation, acid production, and gas production D 81 @ Assimilation of carbon sources other than sugar ■ Salt tolerance 3wtχ Does not grow in NaC1-containing medium.

[Phase] Vitamin requirement Absolutely does not require vitamins.

■ Denitrification reaction Negative.

@GC (guanine + cytosine) content Tl1-3 63.5 molχDM
-8163,2mol! O Main bacterial fatty acid composition Monounsaturated fatty acids C, a.

■ Major hydroxy acids 3-Hydroxy acid CIZ+.

[Phase] Quinone type Ubiquinone QI.

[Phase] Separation source Soil.

Among the mycological properties, these seedlings have polar flagella, form white or yellow-white colonies, are Durham-negative monomethylamine-assimilating bacteria, and have a GC content of 63.
5mol! or 63.2 molχ, with monounsaturated fatty acid Cl811 as the main bacterial fatty acid composition, and 3-hydroxy acid CIti. is the main hydroxy acid, and the quinone type is ubiquinone Q1°, so it is classified as oil-based and komagata J, Gen, Micro-biol,
'32.317-341 (1986)), a group 1 monomethylamine-assimilating bacterium, Pseudomonas aminovorans.
The strain was identified as belonging to S. orans.

Note that NIJB 9039 is a strain that succumbs to Pseudomonas aminovorans, but this seedling strain did not actively assimilate or degrade dimethylamines.

In addition, the paper by Yugami and Komagata [“J, Gen, Appl.
, Micro-biol”32.317-341 (1
986) and C"J, Gen, Appl-Mic
robiol,” 33.135~I65 (19B?
)) is a close relative of Pseudomonas aminovorans, and Pseudomonas sp. TK 3002 (JT
CC2389=NCII311509), same TK 30
03 (=ATCC25262), but these two strains were also unable to actively assimilate or degrade dimethylamines.

As a representative example of a bacterial strain belonging to the genus Arthrobacter, Arthrobacter globiformis JCM 1332 (=AT
CC8010,, NCIB 8907=IF01213
7) and 11"012438.

As a strain belonging to the genus Mycobacterium, Mycobacterium methanolica P-2, which the present inventors isolated from soil,
3 (FEI Research Institute No. 8825), P-70 (FEI Research Institute No. 9496), Tl+-30 (FEI Research Institute No. 9465 and T11-35 (FEI Research Institute No. 9496), No. 94
No. 97).

The orchidological properties of these strains are shown below.

1. At 30°C for both the liquid broth medium and agar agar medium.
The cells were cultured for 3 days.

■ Normally short rod width 0.5-0.8I! m length 1~3 yuan. ■ type of dividing cells are observed.

■ Motility None.

■ Presence or absence of spores Not produced.

■ Durham staining positive.

■ Anti-acidity positive.

2. Growth status in each of the following media (cultured at 37°C for 3 days unless otherwise specified) ■ Medium growth on broth agar plate medium.

Colony morphology and properties: External shape - circular, size 12-3+r++n.

Ridges - hemispherical, structure - homogeneous, surface - rough.

Margin: Wavy 9 colors: Yellowish white, no gloss.

Transparency - opaque, hardness - buttery.

■ Methanol-containing agar plate medium Same as in broth agar plates.

■ Broth agar slant medium Grows vigorously and uniformly along the inoculation line.

Colony morphology and properties: Ridges - Moderate 1 Surface - Rough 1 Margin - Wavy.

Color: Yellow-white, non-glossy; Transparency: Opaque.

Hardness - buttery.

■ Methanol-containing agar slant medium Same as in broth agar slants.

■ Gravy liquid medium forms a white cream-colored closed ring. It also forms a film.

■ Peptone water liquid medium Same as in broth liquid medium.

■ Grows vigorously in liquid medium containing methanol. Forms a white cream-colored closed ring. It also forms a film.

■ Meat juice gelatin puncture culture Cultured at 20°C for 4 weeks.

Grow. However, it does not liquefy gelatin.

■ Ridmus milk Cultured at 37°C for 4 weeks.

The culture medium grows alkaline (pl+ 6.8 →
pl+ 6.8) but without peptonization.

[Phase] 1χ Ogawa medium Grows vigorously. The colony is smooth in appearance.

■ HA medium (hydroxylamine hydrochloride 500 parts/mβ
Supplemented with 1χ Ogawa medium) Cultured at 37°C for 5 days.

P-23 and P-70 grow vigorously.

TH-30 and TH-35 grow weakly.

@ PAS medium (sodium aminosalicylate 2
1χ Ogawa medium supplemented with mg/ml) and cultured at 37°C for 7 days.

It grows vigorously and the medium turns black.

However, only P-23 does not turn the medium black.

■ Picric acid medium (modified method 5a with 0.2χ picric acid addition)
Uton agar medium) Cultured at 37°C for 2 weeks.

It grows vigorously and the medium turns reddish brown.

However, only with P-23, the medium does not turn reddish brown.

@ PNB medium (500 μg paranitrobenzoic acid /
Cultured at 37°C for 7 days (added 1ml Ogawa medium).

Grows vigorously.

■ EB medium (5 H/ml of ethambutol! added 1
2 Ogawa medium) Cultured at 37°C for 7 days.

Grows vigorously.

3. Physiological properties ■ Reduction of nitrate Reducing nitrate to nitrite.

■ MR test Negative.

■ VP test Negative.

■ Indole production Negative.

■ Production of hydrogen sulfide Positive.

■ Starch hydrolysis Negative.

■ Use of nitrogen sources Ammonium salts, nitrates, urea and peptone are used as nitrogen sources.

■ Pigment generation　　　　　　　　No generation.

■ Urease positive.

[Phase] Catalase Positive.

■ Production of ammonia Produce.

@ Denitrification reaction Negative.

■ Oxidase Negative.

00-F Test (HughLei
fson method) Negative.

■Growth range: Grows in a pH range of 5 to 9. A pH range of 6 to 8 is preferred.

It does not grow at temperatures of 5°C and 143°C. Temperature lO~40
"C is preferred.

■　Attitude towards oxygen　　　Aerobic.

@it, assimilation and acid production (a) f1
(b) Acid production + (w): It is weak, but it is produced.

■　Il! Assimilation of carbon sources other than carbon B: Peijiaoyan turns black.

■ Salt resistance Grows vigorously in a medium containing 3wtχNacl.

It does not grow vigorously in a medium containing 6wL% NaCl.

[Phase] Vitamin requirement Absolutely does not require vitamins.

■ Photochromic test Negative.

■ Dark color test Negative.

@ Tween 80 hydrolysis test P-23, P-10 negative.

Tl1-30, Tl-35 slightly positive.

[Phase] Contains mycolic acid. Positive.

@GC (guanine + cytosine) money P-2367, 2 molχ r'-70, 67.2 molχ
Tit-3066,0mol! Tl-3566', 4 molX @ Major bacterial fatty acid composition Direct fatty acid C36.

Monounsaturated fatty acid Cl6: IIcI□110 Methyl fatty acid IQ-methyl C+q+.

0 Quinone type Menaquinone MK-9 (H
□) [Phase] Contains cell wall structure meso-diaminopimelic acid.

@ Separation source Soil.

``Birshi's Manual Op Systematic''
Bacteriology Bergey's Manual
of Systematic Bacteriology
'''Volume 2 1 Editor Sneath, Maxr, Sharpe and float 'Williams & Wilkins
(1986)), these strains are bacilli, non-motile, Durham positive, acid-fast, contain mycolic acid, and are aerobic.
It was determined that the bacteria belonged to the genus Mycobacterium.

This is further supported by various aspects such as GC content, cell fatty acid composition, quinone type, and cell wall structure.

Furthermore, these strains have been disclosed in a patent application (Japanese Patent Application No. 61-15156) based on the invention made by one of the present inventors.
It is very similar to Mycobacterium methanolica disclosed in 5) and was identified as Mycobacterium methanolica.

In the present invention, the experimental method for investigating mycological properties is described in Bergey's Manual of Bacteriology.
of 5yste+++atic Bacterio
Krieg and Holt (1984)) J, 'Birshi's Manual of Systematic Bacteriology'
ergey's Manual of Systema
tic Bact-eriology' Volume 2'', edited by the Institute of Medical Science Alumni Association, ``Bacteriology Practical Summary J (1958),'' and ``Classification and Identification of Microorganisms J'' (1958) by Niji Akebono Hase.
975).

A methanol-containing agar plate medium and a methanol-containing agar slant medium were prepared as follows.

That is, (NH4)2S043g, KIl, r'0
41.4g, Nazllr'Oa2.1g, MgS
O4'711□00.2g+ CaC1z'2tLzO
30mg.

FeC6H507'XIIzO30mg, MnCl2
'4Hz05mg, ZnSO4°7+1.05mg,
After dissolving 0.51 ng of CuSO4・5HzO and 0.2 g of yeast extract in 12 parts of water and adjusting pl+ to 7.1, 15 g/7 of agar was further added, and this was dissolved by heating, followed by 8 g/7 of methanol. ! and then 1
Sterilized at kg/ci G for 20 minutes.

As the methanol-containing liquid medium, a medium with the same composition as the methanol-containing agar plate medium and the methanol-containing agar slant medium without adding agar was used.

Furthermore, a monomethylamine-containing agar plate medium and a monomethylamine-containing agar slant medium were prepared as follows. That is, (NH4) 2S043 g.

KIIZPO41.4g, NaztlPO42.1g
+ MgSO4'7Hz00.2gt CaC1z'2
tLzO30mg, FeC6H5O, °X1(zo
30mg, MnC1z'4tlzO5mg1ZnSO
i°7H205mg, CuSO4'5Hz00.5m
g.

Yeast extract 0.2g and monomethylamine hydrochloride 5g
After dissolving in 1 liter of pure water and adjusting the pH to 7.1,
Furthermore, 15 g/l of agar was added and dissolved by heating.
It was sterilized with 1 kg/c+flG for 20 minutes.

As the monomethylamine-containing liquid medium, a medium with the composition of the monomethylamine-containing agar slant medium and the monomethylamine-containing agar slant medium without adding agar was used.

Note that an aqueous solution containing dimethylamine is alkaline, so in order for bacteria to efficiently assimilate and decompose dimethylamine, the pH of this aqueous solution must be adjusted to approximately neutral level using an acidic substance such as hydrochloric acid or sulfuric acid. It is preferable to make it a gender. By adjusting the PL+ of this aqueous solution to neutrality, dimethylamine is changed into dimethylamine salts such as dimethylamine hydrochloride and dimethylamine sulfate, and is more easily assimilated and decomposed by bacteria. It is presumed that dimethylamine in this aqueous solution is more easily removed.

In order to detoxify a liquid containing dimethylamines (hereinafter sometimes referred to as the liquid to be treated), various nutritional components are added to the liquid to be treated, and this is used as a culture medium to treat liquids belonging to the above-mentioned genera. There is a method of culturing bacteria (hereinafter referred to as "the bacteria") that can assimilate and decompose dimethylamines.

That is, the nutritional components added to the liquid to be treated are not particularly limited as long as they can be assimilated by the bacteria used, and include carbon sources, nitrogen sources, inorganic components, and other components.

Dimethylamines alone in the liquid to be treated may be used as the carbon source, but other carbon sources that can be used by the present bacteria may also be used.
For example, tin such as glucose, fructose and galactose, sugar alcohols such as D-sorbitol and D-mannitol, and alkylamines such as monomethylamine can also be used in combination.

As nitrogen sources, use is made of inorganic nitrogen compounds, such as, for example, ammonium salts and nitrates, and/or organic nitrogen-containing substances, such as, for example, urea, corn steep liquor, casein, peptone and meat extract.

Note that dimethylamines are nitrogen compounds, so no other nitrogen sources are added (but these bacteria can grow and proliferate sufficiently and are able to assimilate and decompose dimethynthylamines). .

In addition, examples of inorganic components include calcium salts, magnesium salts, potassium salts, sodium salts, phosphates,
Manganese salts, zinc salts, iron salts, copper salts, molybdenum salts, cobalt salts, boromine compounds, iodine compounds, and the like are used.

Furthermore, when using a bacterial strain that requires nutritional substances such as vitamins, the nutritional substances required by the strain are added.

The concentration of dimethylamines in the liquid to be treated is not particularly limited as long as it can be assimilated and decomposed by the bacteria used, but it is usually 1 wt% as dimethylamine.
The following is preferable, and 0.5wtX or less is particularly preferable.

Among the strains belonging to Mycobacterium methanolica, there are strains that are particularly resistant to dimethylamines, but when using such strains, the upper limit of the concentration of dimethylamines in the liquid to be treated should be The amine may be about 2wtZ.

The culture conditions may be any conditions that allow the bacteria used to grow and multiply, but generally the temperature is, for example, 5 to 45 degrees.
C1 Preferably, the temperature is 10-40°C, and the pH is 6-8
, preferably 6.5 to 7.5.

Culture is carried out aerobically under these conditions.

In addition, the dissolved oxygen concentration of the culture solution is low enough for this bacterium to grow.

There is no particular restriction on the dissolved oxygen concentration as long as it allows proliferation, but usually about 0.5 to 20 ppm is preferable. In order to achieve such a dissolved oxygen concentration, it is necessary to adjust the amount of aeration gas, stir it, use oxygen gas or a mixed gas of oxygen and air as the aeration gas, and adjust the pressure inside the culture tank. Measures such as increasing the

Although the growth and proliferation of this bacterium will be relatively poor and the efficiency of dimethylamine removal and decomposition will be relatively reduced, this does not preclude culturing under these conditions.

Furthermore, the culture method may be batch culture, continuous culture, or semi-continuous culture.

When an ammonium salt or an alkylamine salt is used as a nitrogen source, ammonia is consumed for bacterial cell production during the culture period, resulting in a decrease in pl+ of the culture solution. In this case, in order to maintain the pl+ of the culture solution at a predetermined value,
Alkali such as ammonia, caustic potash and caustic soda are added, preferably ammonia.

In addition to the method of making the liquid to be treated harmless by culturing the bacteria using the liquid to be treated as a medium as described above,
Pre-cultured cells of this bacterium and processed materials of this bacterium - for example, culture solution containing cells of this bacterium, crushed cells of this bacterium, and cells of this bacterium are prepared using synthetic resin, etc. Immobilized microbial cells (hereinafter sometimes referred to as "microbial cells") can also be brought into contact with the liquid to be treated.

For example, (a) adding microbial cells to the liquid to be treated; (c) bringing the liquid to be treated into contact with activated sludge mixed with the bacterial cells, culture solution, and crushed bacterial cells; and (c) There are methods such as passing the liquid to be treated through a column filled with immobilized bacterial cells.

In addition, since wastewater containing dimethylamines often also contains other substances, when treating such wastewater, a strain of bacteria that can decompose other substances should be used together with this bacterium. They can be used together and are preferred.

After the treatment is completed, the treated liquid in which the concentration of dimethylamine in the liquid to be treated has become below the permissible concentration may be discharged as is or after removing the bacterial cells and/or the bacterial treated material as necessary. Ru.

[Example] The present invention will be explained in more detail with reference to Examples. Note that the present invention is not limited to the examples.

Example 1 Per 1j2 of pure water, ffH4) zsO43g, KH
Hot PO4t, 4g+NaJPO42, 1g9MgSO4
'7HgO0,2g, CaC1z'2tlzO30
mg + FeCbHs (H, 030 mg to Jro,
MnC1z'4Hz05mg, Zn5Q4"711z
05mg, Cu5Oa'511tOO, 5■, yeast extract 0.2g and dimethylamine hydrochloride 2.8g were added pi! Various strains were inoculated into a medium adjusted to 7.1, cultured at 30°C for 14 days, and the ability of each strain to assimilate dimethylamines was examined. The results are shown in Table 1.

As shown in Table 1, each strain assimilated and degraded dimethylamine hydrochloride. (Margin below) Table 1 (Part 1) Table 1 (Part 2) Table 1 (Part 3) Example 2 Per 11 pure water, (NHs)zsO43g, KHzP
o, 1.4g.

NazHPO42,1g 1MgSO4・711zOO
,2g, CaC1z・2HJ30mg, PeCJ
507・XH2O30mg, MnC1z'4Hz05
mg, Zn5On.

IHzo 5mg, CuSO4'5HzOo, smg
A medium containing 0.2 g of yeast extract and adjusted to pH 7.1 was used as a basal medium, and a solution adjusted to pH 7.0 by adding 10 wt χ dimethyldimethylamine concentration was used as a basal medium, and a dimethylamine concentration of 0. .15wLX, 0.
3wtχ, 0.5wtX, 1wtL1.5wtχ,
A medium was prepared by adding 2wjχ, 3wtχ and 5wtχ. For each of these media,
Each strain was inoculated and cultured at 30°C for 7 days.

The results are shown in Table 2. According to this result, Protomonas extroquens has a dimethylamine concentration of 1.0 i
vt% or less, and both Pseudomonas aminovorans and Arthrobata globiformis assimilated and decomposed dimethylamines at a dimethylamine concentration of 0.5 wL2 or less.

In addition, four strains belonging to Mycobacterium mekunorichi assimilated and degraded dimethylamines even at a dimethylamine concentration of 3wtχ. Particularly, when it was less than 2 wt%, it was actively assimilated and degraded. (Margin below) Table 2 (Part 1) Table 2 (Part 2) Example 3 Per 11 pure water, (NI+4) zsOa 3 g,
KH2PO41.4g.

NazHPOa 2.1 g, Mg5Oa°7)1
zo 0.2 g, CaC1z' 211z030
mg, FeCa1lsO7・XHzO30mg, M
nC1z'4Hz05mL ZIISO4'7Hz05
200 mN of medium adjusted to ptt'y, i by adding 50 ml of a solution adjusted to pH 7.0 by adding hydrochloric acid to a 10-tχ dimethylamine aqueous solution, 200.5 mg of CLISO4°5H, 0.2 g of yeast extract, and 10-tχ dimethylamine aqueous solution. 1
! The mixture was placed in an Erlenmeyer flask and sterilized at 120°C for 20 minutes.

Into this medium, a pre-culture solution of Mycobacterium methanolica T11-30 (bacterial cell concentration OD610., -2.0) obtained by culturing in advance in the same medium as above was inoculated to 1 volχ. Cultured at 30°C for 40 hours, resulting in bacterial cell concentration (expressed as OD,...)
and the pn of the culture solution are 5.8. and 5.
It was 1. Furthermore, no dimethylamines were detected in the culture supernatant.

Note that the generation time was approximately 4.5 hours.

Example 4 From the composition of the basal medium in Example 3, (NI +
4) Example 3 was added to the solution, which had been adjusted to pH 7.0 by adding nutritional components so as to have a medium composition excluding zsOa.
1 g of Mycobacterium methanolica Tl+-30 cells isolated from a preculture solution obtained by culturing in a medium similar to the above was suspended, and the pH and temperature of the culture solution were adjusted while aerating and stirring. , 7.0 and 30°C, respectively
I kept it.

It took about 20 hours until dimethylamines were no longer detected in the factory effluent.

In each of the above Examples, dimethylamine in the liquid was analyzed using Toyo 5oda ion chromatography.

Pump: Toyo 5oda CCPD detector: T
oyo 5oda CM-8000 column: TSKg
el IC-Cation (Toyo 5oda)
[Effects of the Invention] The present invention makes it possible to efficiently decompose and remove dimethylamines, which are stable, difficult to decompose, and harmful substances, and thereby efficiently remove harmful wastewater containing dimethylamines. It can be rendered harmless and has extremely high value in terms of preserving environmental health.

Patent applicant: Mitsubishi Gas Chemical Co., Ltd. Representative Nagano Japanese book

Claims (1)

[Claims] A liquid containing dimethylamine and/or a dimethylamine salt, belonging to any of the genera Methylobacillus, Protomonas, Methylophaga, Pseudomonas, Arthrobacter and Mycobacterium, and dimethylamine and/or dimethylamine. Dimethylamine and/or dimethylamine salts in the liquid are decomposed and removed by contacting with bacterial cells that can assimilate and decompose salts and/or a bacterial cell-treated product of the bacteria. How to remove dimethylamines.